Pol polyprotein-like protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033557 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Pol polyprotein-like protein vs. Ensembl Zebrafish
Match: CABZ01030006.1 (pep scaffold:GRCz11:KN150258.1:33006:36502:-1 gene:ENSDARG00000114113.1 transcript:ENSDART00000182402.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01030006.1) HSP 1 Score: 75.0998 bits (183), Expect = 3.239e-15 Identity = 51/161 (31.68%), Postives = 75/161 (46.58%), Query Frame = 2 Query: 47 CKNCHVSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELP 526 C C V+K N K L++ R ++ +DIMGP+P + +Y+ +D + W E P+ ++ + E +TR GV I+ QF+SS F ELC K+NI TT Q N ER +RTLKS + ED ++ LP Sbjct: 12 CAKCQVTKWDNRKP------AGKLQQVTTSRPNEMWRVDIMGPMPKSGKQNEYLLVFVDYFSKWVELFPMRHATAQTIATILRQEMLTRWGVPDFILSDRGAQFVSSLFTELCGKWNITPKLTTAYHPQTN-MTERVNRTLKSMIAGFVEDNHKTWDTYLP 165
BLAST of Pol polyprotein-like protein vs. Ensembl Xenopus
Match: mybl2 (MYB proto-oncogene like 2 [Source:Xenbase;Acc:XB-GENE-982419]) HSP 1 Score: 63.929 bits (154), Expect = 3.920e-11 Identity = 40/135 (29.63%), Postives = 68/135 (50.37%), Query Frame = 2 Query: 143 FKVIHMDIMGPL-PLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ I MD++GP+ ++I I+D T + EAIP+ SS R + + + +R G+ K I+ F+S ELC + + + T+ Q + VERF++TLKS L+ + +N LP ++ Sbjct: 146 FERIAMDLVGPIVKSARGHQHILVIMDYATRYPEAIPLRNTSSKSIARELVHVF-SRVGIPKEILTDQGTPFMSRVMKELCRLFKVVQLRTSVYHPQTDGLVERFNKTLKSMLKKVVDKDGKNWDYLLPYLMFAI 279
BLAST of Pol polyprotein-like protein vs. Ensembl Xenopus
Match: nmbr (neuromedin B receptor [Source:Xenbase;Acc:XB-GENE-6074611]) HSP 1 Score: 62.003 bits (149), Expect = 1.573e-10 Identity = 37/114 (32.46%), Postives = 61/114 (53.51%), Query Frame = 2 Query: 143 FKVIHMDIMGPL-PLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLR 481 F+ I MD++GP+ ++I I+D T + EAIP+ SS R + + + +R G+ K I+ F+S ELC + + + T+ Q + VERF++TLKS L+ Sbjct: 213 FERIAMDLVGPIVKSARGHQHILVIMDYATRYPEAIPLRNTSSKSIARELVHVF-SRVGIPKEILTDQGTPFMSRVMKELCRLFKVVQLRTSVYHPQTDGLVERFNKTLKSMLK 325
BLAST of Pol polyprotein-like protein vs. Ensembl Xenopus
Match: ENSXETT00000023941.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV463742.1:21:4282:-1 gene:ENSXETG00000015734.1 transcript:ENSXETT00000023941.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 56.225 bits (134), Expect = 2.468e-8 Identity = 38/136 (27.94%), Postives = 66/136 (48.53%), Query Frame = 2 Query: 143 FKVIHMDIMGPL--PLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ + +D++GPL P S K+YI ++D T + EA+ + ++ + T+ + +R G I+ QF S L + + IH++ Q N ERF+ TLK+ LR E ++ LP +L + Sbjct: 708 FQRVAVDLIGPLSRPSRSGKQYILTVMDYATRYPEAVALRKIDAP-TVADALIQIFSRVGFPSEILSDQGPQFTSQLLQCLWQRCGVRAIHSSPYHPQTNGLCERFNGTLKTMLRTFVESGEKDWERYLPHLLFAY 842
BLAST of Pol polyprotein-like protein vs. Ensembl Xenopus
Match: ENSXETT00000023851.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV461115.1:7997:9051:-1 gene:ENSXETG00000015824.1 transcript:ENSXETT00000023851.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 3.992e-7 Identity = 34/136 (25.00%), Postives = 63/136 (46.32%), Query Frame = 2 Query: 143 FKVIHMDIMGPL--PLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ + +D++GPL P + K+YI ++D T + EA+ + + + + + +R G I+ QF+S L + + + ++ Q N ERF+ TLK+ LR E + LP +L + Sbjct: 155 FQRVAVDLIGPLSRPSRTGKQYILTMVDYATRYPEAVALRRIDAPAVADAL-IQIFSRVGFPSEILSDQGPQFMSQLLQCLWQRCGVTSLRSSPYHPQTNGLCERFNGTLKNMLRTFVEAGEGDWEKFLPCLLFAY 289
BLAST of Pol polyprotein-like protein vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1) HSP 1 Score: 53.5286 bits (127), Expect = 9.218e-7 Identity = 36/116 (31.03%), Postives = 56/116 (48.28%), Query Frame = 2 Query: 143 FKVIHMDIMGPLPLVSN-KKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAH 487 F + +D +GPLP N +Y +I T + AIPI S+ + I +I + G KT I ++ +S +LC I++I +T Q VER HRTL +R++ Sbjct: 965 FDRVVVDTIGPLPKSENGNEYAVTLICDLTKYLVAIPIANKSAKTVAKAIFESFILKYGPMKTFITDMGTEYKNSIITDLCKYLKIKNITSTAHHHQTVGVVERSHRTLNEYIRSY 1080
BLAST of Pol polyprotein-like protein vs. UniProt/SwissProt
Match: sp|Q09575|YRD6_CAEEL (Uncharacterized protein K02A2.6 OS=Caenorhabditis elegans OX=6239 GN=K02A2.6 PE=4 SV=1) HSP 1 Score: 52.7582 bits (125), Expect = 1.607e-6 Identity = 36/109 (33.03%), Postives = 52/109 (47.71%), Query Frame = 2 Query: 143 FKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLK 469 +K IH+D GPL N Y+ ++D T + E +S+ TI +E E + G +TII N Q S F ++C + IEH + + N ERF TLK Sbjct: 849 WKRIHIDFAGPL----NGCYLLVVVDAKTKYAEVKLTRSISAVTTIDLLE-EIFSIHGYPETIISDNGTQLTSHLFAQMCQSHGIEHKTSAVYYPRSNGAAERFVDTLK 952
BLAST of Pol polyprotein-like protein vs. TrEMBL
Match: A0A182GF01 (Integrase catalytic domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG008772 PE=4 SV=1) HSP 1 Score: 115.546 bits (288), Expect = 2.519e-26 Identity = 59/135 (43.70%), Postives = 80/135 (59.26%), Query Frame = 2 Query: 140 KFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 +F+ IH+D++GPLP +Y+ IIDR T W EA+P+ EM++ + + WI+R G +TI QF S F EL + HIHTT Q N VERFHRTLK++LR+ S+ N + LPLVLLG Sbjct: 64 RFRNIHIDLVGPLPPSRGHRYLLTIIDRFTRWAEAVPLPEMTAPTVAKALLVGWISRFGTPETITSDQGRQFESEVFRELTSLLGVHHIHTTAYHPQSNGIVERFHRTLKASLRSASDS---NWYDRLPLVLLGL 195
BLAST of Pol polyprotein-like protein vs. TrEMBL
Match: A0A0A9YG17 (Pro-Pol polyprotein (Fragment) OS=Lygus hesperus OX=30085 GN=pol_21 PE=4 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 9.140e-25 Identity = 54/147 (36.73%), Postives = 78/147 (53.06%), Query Frame = 2 Query: 104 IKSNLKEFLECRKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 +K+ L+ KF+ IH+D++GPLP Y +IDR+T WTE P+ + + T+ EWI+R GV K I QF S+ F +C+ I T + Q N +ERFHR LK++L+ + N N N LP V+LG Sbjct: 8 VKAPLEPIYTGNKFETIHIDLIGPLPASQGYNYCLTVIDRNTKWTECYPVRNIEANTITETLYKEWISRFGVPKIITTDQGRQFESAIFKSMCVTLGINRHRTNSFHPQSNGMIERFHRNLKTSLKPQIQ--NSNWVNNLPTVMLGL 152
BLAST of Pol polyprotein-like protein vs. TrEMBL
Match: A0A2L2YVP0 (Integrase catalytic domain-containing protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1) HSP 1 Score: 113.62 bits (283), Expect = 1.048e-24 Identity = 70/175 (40.00%), Postives = 88/175 (50.29%), Query Frame = 2 Query: 23 MTALIRNWCKNCHVSKK*NNKT*NLYDIKSNLKEFLEC-RKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 MT IR W K C +K KS E+ E +F ++H+D++GPLP K Y IDR T W EAIPI ++++ + R WITR GV II QF S F L + + HTT Q N KVERFHRTLK+ ++AHS N LP VLLG Sbjct: 265 MTKEIREWAKTCIQCQKVKIHR----HTKSQFSEYQEPDARFSMVHIDLIGPLPPSEGKMYCLTCIDRYTCWMEAIPIPDITANIVSRAFYENWITRFGVPTQIITDQGRQFESQLFRSLAVICGAKVQHTTPYHPQSNGKVERFHRTLKTAIKAHS---NIRWTESLPTVLLGL 432
BLAST of Pol polyprotein-like protein vs. TrEMBL
Match: A0A182GDC4 (Integrase catalytic domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG007977 PE=4 SV=1) HSP 1 Score: 109.383 bits (272), Expect = 2.906e-24 Identity = 61/166 (36.75%), Postives = 87/166 (52.41%), Query Frame = 2 Query: 47 CKNCHVSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 C +C +SK N + + + + +F+ IH+D++GPLP +Y+ IIDR T W EAIP+ +M++ + + WI+R G +TI QF S F EL HIHTT Q N VERFHRT+K+ L + + + ELPLVLLG Sbjct: 12 CLDCQLSKV------NRHTVSPYVPFDMPKSRFRHIHIDLVGPLPPSDGNRYVLTIIDRFTRWPEAIPLSDMTAPTVAKALTLGWISRFGTPETITSDQGRQFESDLFRELTYILGTHHIHTTAYHPQANGMVERFHRTMKAALMCKNV---KRWSEELPLVLLGL 168
BLAST of Pol polyprotein-like protein vs. TrEMBL
Match: A0A182GJA0 (Integrase catalytic domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG010915 PE=4 SV=1) HSP 1 Score: 109.383 bits (272), Expect = 3.092e-24 Identity = 67/168 (39.88%), Postives = 91/168 (54.17%), Query Frame = 2 Query: 47 CKNCHVSKK*NNKT*NLYDIKSNLKEF--LECRKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 C+ C +SK + T S L F +CR F+ IH+D++GPLP +Y+ I+DR T W EAIP+ +M++ R + W++R G +TI QF S F EL + HI TT Q N VERFHRTLK+ L ++ NR +ELPLVLLG Sbjct: 12 CQECQLSKVNRHTT-------SPLGSFDPPKCR-FRNIHVDLVGPLPPSKGNRYLLTILDRFTRWPEAIPLSDMTAPTVARALTEHWVSRFGTPETITTYQGRQFESDLFRELTSILGVHHIRTTAYHPQSNGMVERFHRTLKAALMC--KNTNR-WCDELPLVLLGL 168
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000058127.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:35454045:35455481:1 gene:ENSAMXG00000038477.1 transcript:ENSAMXT00000058127.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 75.0998 bits (183), Expect = 4.481e-15 Identity = 48/173 (27.75%), Postives = 80/173 (46.24%), Query Frame = 2 Query: 35 IRNWCKNCH--VSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 + WC+ C S+K + ++ L + R ++ I +DI+GPLP K +YI + D + WTEA + + + + EWI R G ++I F S F ELC N+ T+ + Q + +ERF+RTL S L + ++ LP V++ + Sbjct: 40 VERWCRECTDCASRKTSGPA-----PRAPLLPSVTSRPYERIALDILGPLPETPQKNRYILVVGDYFSKWTEAFSLPNQEAKTVAKVLTEEWICRYGAPRSIHTDQGRNFESHLFSELCRLLNMHKSRTSPYRPQSDGLIERFNRTLLSMLSLFVDANQQDWDALLPFVMMAY 207
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000031920.1 (pep primary_assembly:Astyanax_mexicanus-2.0:12:11094903:11101818:1 gene:ENSAMXG00000039354.1 transcript:ENSAMXT00000031920.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.9442 bits (180), Expect = 7.297e-15 Identity = 48/170 (28.24%), Postives = 79/170 (46.47%), Query Frame = 2 Query: 44 WCKNCH--VSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 WC+ C S+K + ++ L + R ++ I +DI+GPLP K +YI + D + WTEA + + + + EWI R G ++I F S F ELC N+ T+ + Q + +ERF+RTL S L + ++ LP V++ + Sbjct: 7 WCRECTDCASRKTSGPA-----PRAPLLPSVTSRPYERIALDILGPLPETPQKNRYILVVGDYFSKWTEAFSLPNQEAKTVAKVLTEEWICRYGAPRSIHTDQGRNFESHLFSELCRLLNMHKSRTSPYRPQSDGLIERFNRTLLSMLSLFVDANQQDWDALLPFVMMAY 171
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000037741.1 (pep primary_assembly:Astyanax_mexicanus-2.0:6:27788674:27792885:-1 gene:ENSAMXG00000029895.1 transcript:ENSAMXT00000037741.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 68.5514 bits (166), Expect = 6.663e-13 Identity = 45/171 (26.32%), Postives = 75/171 (43.86%), Query Frame = 2 Query: 35 IRNWCKNCHVSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVS-NKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 + +WCK C + + ++ L+ F+ + +DI+GPLP+ S KYI I D T W EA + + RT+ ++I R G ++I F S+ F +C +I TT + VER +RT+ + L + N LP V++ + Sbjct: 124 VMDWCKKCELCAVFKTSG---AEPRAPLRPIQAGYPFEKLGIDIVGPLPVTSQGNKYIIVIADYFTKWCEAFAMRAQDADTVARTVVEQFILRWGTPRSIHTDQGKNFDSALFRAICELLDIHKTRTTPYHPSSDGLVERLNRTITTMLSMFVDANQGNWDELLPYVMMAY 291
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000044431.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000182.1:892613:897532:1 gene:ENSAMXG00000038169.1 transcript:ENSAMXT00000044431.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.781 bits (164), Expect = 1.990e-12 Identity = 48/177 (27.12%), Postives = 89/177 (50.28%), Query Frame = 2 Query: 23 MTALIRNWCKNCHVSKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVS-NKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWI---TR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLG 541 M A +RN+C+ C V + + + + + F+ + MD++GPLP + +Y+ ++D T + EA+ + + ++ R+I E + +R G+ K I+ F+S+ E+C I+H+HT+ Q + VER+++TLK LR ++ RN LP +L Sbjct: 389 MEAEVRNFCQRCAVCQSTSPRK---PAPAPLIPLPIIGVPFERLGMDLVGPLPKSARGHEYLLVLVDYATRYPEAVALRKANT----RSIAKELVLLFSRVGLPKEILSDQGTPFVSNLMKEVCRLLKIKHLHTSIYHPQTDGLVERYNQTLKRMLRRLIDEDGRNWDLLLPYILFA 558
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000039384.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:21146036:21149703:-1 gene:ENSAMXG00000035834.1 transcript:ENSAMXT00000039384.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.781 bits (164), Expect = 2.310e-12 Identity = 49/173 (28.32%), Postives = 80/173 (46.24%), Query Frame = 2 Query: 35 IRNWCKNCHV--SKK*NNKT*NLYDIKSNLKEFLECRKFKVIHMDIMGPLPLVSN-KKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 ++ WC+ C V + K KT ++ L + R F+ I +DI+GPLP+ + KYI I D + W E+ I + R + +++ R G+ + I F SS F ELC I+ T+ Q + VER +RT+ L + E + LP V++ + Sbjct: 463 VKEWCRVCEVCATHKIVGKT-----PRAPLMPSVATRPFQRIALDIVGPLPVTATGNKYILVIADYFSKWAESYAIPNQEATTVARAVV-DFLLRWGMPQVIHTDQGRTFESSLFKELCRLLEIDKTRTSPYSPQSDGLVERLNRTIIGMLASFVEANQNSWDTLLPFVMMAY 629
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Match: ENSORLT00000045127.1 (pep primary_assembly:ASM223467v1:10:587855:593081:1 gene:ENSORLG00000029202.1 transcript:ENSORLT00000045127.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.8037 bits (203), Expect = 6.134e-18 Identity = 49/144 (34.03%), Postives = 72/144 (50.00%), Query Frame = 2 Query: 116 LKEFLECRKFKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 ++ + R F+ + ++I+GPLP NK KYI I D + WTEA P+ + + + EW+ R G ++I F S+ F ELC +I T+ Q + VERF+RTL S L ED N LP V+L + Sbjct: 29 MQTLVPSRPFERVALEILGPLPETPNKNKYILVISDYFSKWTEAFPLPNQEAQSIAKVLTEEWVCRFGAPRSIHSDQGRNFESTLFKELCSLLSIHKSRTSPYHPQSDGLVERFNRTLLSLLALFVEDNQLNWDKLLPYVMLAY 172
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Match: ENSORLT00000030461.1 (pep primary_assembly:ASM223467v1:5:4384046:4385262:1 gene:ENSORLG00000026861.1 transcript:ENSORLT00000030461.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 69.3218 bits (168), Expect = 2.909e-13 Identity = 47/135 (34.81%), Postives = 66/135 (48.89%), Query Frame = 2 Query: 143 FKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ I MD++GPL S+ KYI I D T + EA P+ + + I + + +R GV II F S T +L + I+ I TT Q + VERF++TLK LR D R+ LP VL + Sbjct: 49 FRRIAMDVVGPLEKSSSGYKYILVICDYATRFPEAFPLRSIKTPKIISAL-VQLFSRVGVPDEIITDQATNFTSGTMKQLQKELGIKGIRTTPYHPQTDGLVERFNQTLKQMLRKFVADTGRDWDKWLPFVLFAY 182
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Match: ENSORLT00000033056.1 (pep primary_assembly:ASM223467v1:23:165526:166795:1 gene:ENSORLG00000025483.1 transcript:ENSORLT00000033056.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 69.3218 bits (168), Expect = 3.451e-13 Identity = 47/135 (34.81%), Postives = 66/135 (48.89%), Query Frame = 2 Query: 143 FKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ I MD++GPL S+ KYI I D T + EA P+ + + I + + +R GV II F S T +L + I+ I TT Q + VERF++TLK LR D R+ LP VL + Sbjct: 121 FRRIAMDVVGPLEKSSSGYKYILVICDYATRFPEAFPLRSIKTPKIISAL-VQLFSRVGVPDEIITDQATNFTSGTMKQLQKELGIKGIRTTPYHPQTDGLVERFNQTLKQMLRKFVADTGRDWDKWLPFVLFAY 254
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Match: ENSORLT00000043660.1 (pep primary_assembly:ASM223467v1:15:27996403:27998990:-1 gene:ENSORLG00000029791.1 transcript:ENSORLT00000043660.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 69.3218 bits (168), Expect = 5.928e-13 Identity = 47/135 (34.81%), Postives = 66/135 (48.89%), Query Frame = 2 Query: 143 FKVIHMDIMGPLPLVSNK-KYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 F+ I MD++GPL S+ KYI I D T + EA P+ + + I + + +R GV II F S T +L + I+ I TT Q + VERF++TLK LR D R+ LP VL + Sbjct: 197 FRRIAMDVVGPLEKSSSGYKYILVICDYATRFPEAFPLRSIKTPKIISAL-VQLFSRVGVPDEIITDQATNFTSGTMKQLQKELGIKGIRTTPYHPQTDGLVERFNQTLKQMLRKFVADTGRDWDKWLPFVLFAY 330
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Match: ENSORLT00000042487.1 (pep primary_assembly:ASM223467v1:4:23584510:23586585:1 gene:ENSORLG00000030583.1 transcript:ENSORLT00000042487.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.3958 bits (163), Expect = 2.119e-12 Identity = 39/147 (26.53%), Postives = 66/147 (44.90%), Query Frame = 2 Query: 104 IKSNLKEFLECRKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGF 544 I++ +K FL R +V+ +D P ++ + + D T +T+A P + + T + + EW + GV + + F S ELC Y ++ TT Q N + ERF+RTL LR + R LP ++ + Sbjct: 283 IRTPMKHFLATRPLEVVAVDFTLLEPASDGRENVLVVTDVFTKFTQAFPTRDQKADTTAKVLLREWFMKYGVPERLHSDQGRNFESEVIQELCKLYGVKKTRTTPHHPQGNAQCERFNRTLHDLLRTLPPEKKRRWPEYLPELIYAY 429
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Match: SMESG000055306.1 (SMESG000055306.1) HSP 1 Score: 304.294 bits (778), Expect = 4.453e-106 Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 2 Query: 335 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTNCSPNLKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP 778 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTN SP+LKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP Sbjct: 1 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTNRSPDLKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP 148
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Match: SMESG000054366.1 (SMESG000054366.1) HSP 1 Score: 304.294 bits (778), Expect = 4.453e-106 Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 2 Query: 335 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTNCSPNLKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP 778 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTN SP+LKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP Sbjct: 1 MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYNDKPCLELTVAYLGTSDVWFKEETRTAPKPMLDGLFSVTNRSPDLKTFEIKQPTRTIRVSVGKLKVAYFRDDSLVIP 148
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Match: SMESG000000071.1 (SMESG000000071.1) HSP 1 Score: 73.1738 bits (178), Expect = 1.702e-14 Identity = 51/142 (35.92%), Postives = 68/142 (47.89%), Query Frame = 2 Query: 125 FLECRKFKVIHMDIMGPLPLVSNKKYIFRIIDRDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYN 550 F + F++IH++I+GPL +N Y + L +R WI R G KTI+ QF S F LC YNI+ TT Q N K+ER H+TLK +LR +ED RN N+ P +LLG N Sbjct: 232 FPSRQPFELIHVNIVGPLAPNNN--------------------YRQENKL-VRKFN--WIGRYGQPKTIMTDQVRQFESQLFEMLCKIYNIKRNRTTAFNPQANGKIERLHKTLKESLRTKAED--RNWLNQPPTILLGLRN 348
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Match: SMESG000027384.1 (SMESG000027384.1) HSP 1 Score: 69.707 bits (169), Expect = 5.088e-14 Identity = 39/99 (39.39%), Postives = 58/99 (58.59%), Query Frame = 2 Query: 146 KVIHMDIMGPLPLVSNKKYIFRIID----RDTSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQ 430 K+ +++ G + N+K I+ + TSW EAIPI ++ S +T+E EWI++ + II N QF+SS+F +LC KYNIEH+HTT Q Sbjct: 122 KLAQIEVNGKTNKIRNEKTFMSILLVHYLQSTSWPEAIPIGDIRSETVYKTMEREWISKFESPEIIITDNGKQFISSSFKKLCNKYNIEHLHTTPYHPQ 220
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Match: SMESG000007179.1 (SMESG000007179.1) HSP 1 Score: 69.3218 bits (168), Expect = 3.254e-13 Identity = 43/108 (39.81%), Postives = 55/108 (50.93%), Query Frame = 2 Query: 227 TSWTEAIPIYEMSSGLTIRTIENEWITR*GVFKTIIMGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAHSEDPNRNLANELPLVLLGFYN 550 T+W E I + E+S+ +E WI R KTII + QF LC YN + T Q N K+ER HRTLK +LR +ED RN N+LP +L G N Sbjct: 210 TNWFEVISLKEISAKYICNNLEFNWIARYDQPKTIITDQERQF-----EMLCKNYNFKRNRITAYNPQANGKIERLHRTLKESLRTKAED--RNWLNQLPTILPGQRN 310 The following BLAST results are available for this feature:
BLAST of Pol polyprotein-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Pol polyprotein-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Pol polyprotein-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Pol polyprotein-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of Pol polyprotein-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Pol polyprotein-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Pol polyprotein-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Pol polyprotein-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Pol polyprotein-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033557 ID=SMED30033557|Name=Pol polyprotein-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=816bpback to top protein sequence of SMED30033557-orf-1 >SMED30033557-orf-1 ID=SMED30033557-orf-1|Name=SMED30033557-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp MGNDIQFLSSTFMELCLKYNIEHIHTTTCQSQCNDKVERFHRTLKSTLRAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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