SMED30033552

Overview
NameSMED30033552
Smed IDSMED30033552
Length (bp)837
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033552 (SMED30033552) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033552 (SMED30033552) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033552 (SMED30033552) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033552 (SMED30033552) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033552

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30033552SMESG000046292.1 dd_Smed_v4_16612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30033552SMESG000046292.1 dd_Smed_v4_16612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033552SMESG000046292.1 dd_Smed_v4_16612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30033552SMESG000046292.1 dd_Smed_v4_16612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30033552SMESG000046292.1 dd_Smed_v4_16612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30033552SMESG000046292.1 dd_Smed_v6_16612_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30033552 vs. TrEMBL
Match: A0A504Z6W3 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_10074 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.704e-8
Identity = 49/180 (27.22%), Postives = 79/180 (43.89%), Query Frame = 1 
Query:  124 KLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQT-KCQ--FSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRFCQKH-SQDFTGALVDAGI 651
            KL PT  PC  +  Q+  QC+  E   L   +     +F+ GIF+G+LL IC + +   +C   IN T  CQ   S   +  D  V  I+ +C+  ++YE+Y  +++C+         CY +                  + N C     + DCV  + + FC  + S      LV+AG+
Sbjct:   33 KLPPTPAPCLPQALQLAAQCQNVELSTLIRLLPQDPRAFLFGIFLGQLLQICEAVDHLLQC-DSINLTDSCQRPASWTTQPWDTSVRPIIDLCQDPHLYEKYTVVLNCVKANDDRFRLCYQSPP----------------RPNDCNSVEHIRDCVERQWRMFCLSNPSVQLVPYLVNAGL 195
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BLAST of SMED30033552 vs. TrEMBL
Match: A0A4E0RZY0 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_000728 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.126e-8
Identity = 48/180 (26.67%), Postives = 78/180 (43.33%), Query Frame = 1 
Query:  124 KLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQT-KCQ--FSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRFC-QKHSQDFTGALVDAGI 651
            KL PT  PC  +  Q+  QC+  E   L   +     +F+ GIF+G LL IC + +   +C   IN T +CQ   S      D  V  I+ +C+  ++YE+Y  +++C+         CY +                  + N C +   + DCV  + + FC    S      L++AG+
Sbjct:   33 KLPPTPAPCLPQALQLAAQCQNVELSALIRLLPQDPSAFLFGIFLGHLLQICEAVDHLLQC-DSINLTDRCQRPASWTTPPWDTSVRPIIDLCQDPHLYEKYTVVLNCVKANDDRFRLCYHSPP----------------RPNDCNNVEHIRDCVERQWRMFCLLNPSVQLVPYLINAGL 195
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BLAST of SMED30033552 vs. Planmine SMEST
Match: SMESG000046292.1 (SMESG000046292.1)

HSP 1 Score: 485.723 bits (1249), Expect = 6.474e-176
Identity = 252/254 (99.21%), Postives = 253/254 (99.61%), Query Frame = 1 
Query:   37 MTEIGILYKVXXXXXXXXXXXXYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAKELKQVFSEADSSSNYITHYKHNILSIICFAAIVL 798
            MTEIGILYKVSTLL FLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSR+RILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAKELKQVFSEADSSSNYITHYKHNILSIICFAAIVL
Sbjct:    1 MTEIGILYKVSTLLFFLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRQRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAKELKQVFSEADSSSNYITHYKHNILSIICFAAIVL 254
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BLAST of SMED30033552 vs. Planmine SMEST
Match: SMESG000046292.1 (SMESG000046292.1)

HSP 1 Score: 420.239 bits (1079), Expect = 2.222e-150
Identity = 218/220 (99.09%), Postives = 219/220 (99.55%), Query Frame = 1 
Query:   37 MTEIGILYKVXXXXXXXXXXXXYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAK 696
            MTEIGILYKVSTLL FLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSR+RILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAK
Sbjct:    1 MTEIGILYKVSTLLFFLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRQRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAK 220
[Close]
BLAST of SMED30033552 vs. Planmine SMEST
Match: SMESG000046292.1 (SMESG000046292.1)

HSP 1 Score: 420.239 bits (1079), Expect = 2.610e-150
Identity = 223/247 (90.28%), Postives = 230/247 (93.12%), Query Frame = 1 
Query:   37 MTEIGILYKVXXXXXXXXXXXXYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAKELKQVFSEADSSSNYITHYKHNILSII 777
            MTEIGILYKVSTLL FLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSR+RILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAK    VF          T+++H++  +I
Sbjct:    1 MTEIGILYKVSTLLFFLFLSFTYFEGFKCKLIPTQPPCSTEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYPECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKFSPPIVSCYSTYSNAIMQYVNISSRQRILKSNACKHYFDLYDCVVMESKRFCQKHSQDFTGALVDAGIHFSQICKLNLSSEAK----VF----------TNFRHSLFQVI 233
The following BLAST results are available for this feature:
BLAST of SMED30033552 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A504Z6W32.704e-827.22Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A4E0RZY04.126e-826.67Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
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BLAST of SMED30033552 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033552 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000046292.16.474e-17699.21SMESG000046292.1[more]
SMESG000046292.12.222e-15099.09SMESG000046292.1[more]
SMESG000046292.12.610e-15090.28SMESG000046292.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033552 ID=SMED30033552|Name=SMED30033552|organism=Schmidtea mediterranea sexual|type=transcript|length=837bp
GATATTATTTTAAATAATTTTTCTTTTAAAATTTCAATGACAGAAATAGG
CATTCTGTATAAAGTTTCTACATTGCTATTATTCTTGTTTTTATCTTTCA
CATATTTTGAAGGCTTTAAATGTAAACTCATTCCAACGCAGCCCCCTTGT
TCAACAGAAGATAGGCAAATAGTATATCAGTGCAGACAAGACGAACGACA
TAAATTGAGTTTAAAGATTTCACCAAAAATAGATTCTTTTATCAAAGGCA
TTTTCATAGGGAAATTACTTGACATATGCAATTCAGCCGAAAAATATCCT
GAATGTTTAAAACAAATTAACCAAACCAAGTGCCAATTTTCTGAAATAAT
TGAAGAAATTGATTTCGATGTTGTTTCAATTCTGTCAATGTGCAGATACA
AATATATTTATGAAGAATATAATAGCATAGTAAGTTGTTTGACGAAGTTT
TCACCGCCAATTGTGTCCTGTTACTCAACATATTCAAATGCGATTATGCA
ATACGTTAACATCTCATCAAGGAAACGCATTCTCAAATCCAACGCTTGCA
AGCATTACTTTGATCTCTATGACTGTGTAGTGATGGAATCTAAAAGATTT
TGTCAGAAGCATTCACAAGACTTTACTGGAGCATTAGTAGACGCAGGAAT
ACATTTTTCCCAGATCTGTAAATTAAATCTTTCTTCGGAAGCAAAGGAAT
TAAAGCAAGTTTTTTCTGAAGCCGATTCATCTTCCAATTACATAACTCAT
TATAAACATAATATATTATCAATTATATGTTTCGCAGCAATTGTTTTATA
CAAAATAAAATATTAATAGATTTTTTTCAAAAAAAAA
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protein sequence of SMED30033552-orf-1

>SMED30033552-orf-1 ID=SMED30033552-orf-1|Name=SMED30033552-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=272bp
DIILNNFSFKISMTEIGILYKVSTLLLFLFLSFTYFEGFKCKLIPTQPPC
STEDRQIVYQCRQDERHKLSLKISPKIDSFIKGIFIGKLLDICNSAEKYP
ECLKQINQTKCQFSEIIEEIDFDVVSILSMCRYKYIYEEYNSIVSCLTKF
SPPIVSCYSTYSNAIMQYVNISSRKRILKSNACKHYFDLYDCVVMESKRF
CQKHSQDFTGALVDAGIHFSQICKLNLSSEAKELKQVFSEADSSSNYITH
YKHNILSIICFAAIVLYKIKY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 17..36