SMED30033550

Overview
NameSMED30033550
Smed IDSMED30033550
Length (bp)711
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033550 (SMED30033550) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033550 (SMED30033550) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033550 (SMED30033550) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033550 (SMED30033550) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30033550

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30033550SMESG000052297.1 SMESG000012644.1 SmedASXL_010899SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30033550SMESG000052297.1 SMESG000012644.1 SmedASXL_010899SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
reproductive organSMED30033550SMESG000052297.1 SMESG000012644.1 Contig44866newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30033550SMESG000052297.1 SMESG000012644.1 Contig44866uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033550 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003294:82950..84194 -3418
v31.004003:35098..36339 +3400
Homology
[Close]
BLAST of SMED30033550 vs. Planmine SMEST
Match: SMESG000052297.1 (SMESG000052297.1)

HSP 1 Score: 408.683 bits (1049), Expect = 1.159e-146
Identity = 207/224 (92.41%), Postives = 207/224 (92.41%), Query Frame = 2 
Query:   53 MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLPKKPCVIIEMPVQVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSK--------------VLMNLKMSPGVAKRDFIQLSTKKY 682
            MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLL KKPCVIIEMPVQVNN EDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKSQLKF AMKKFLKPSISSLKCLQDRQLSSK              VLMNLKMSPGVAKRDFIQLSTKKY
Sbjct:    1 MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLHKKPCVIIEMPVQVNNEEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKSQLKFLAMKKFLKPSISSLKCLQDRQLSSKGCLLFSFYSLISFPVLMNLKMSPGVAKRDFIQLSTKKY 224
[Close]
BLAST of SMED30033550 vs. Planmine SMEST
Match: SMESG000012644.1 (SMESG000012644.1)

HSP 1 Score: 407.912 bits (1047), Expect = 2.050e-146
Identity = 205/224 (91.52%), Postives = 207/224 (92.41%), Query Frame = 2 
Query:   53 MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLPKKPCVIIEMPVQVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSK--------------VLMNLKMSPGVAKRDFIQLSTKKY 682
            MCGINNCL+ACISSIL HSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLPKKPCVIIEMPVQV+N EDHDLLVDREKYGILSNVPAIPEGFQDVF LKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSK              VLMNLKMSPGVAKRDFIQLSTKKY
Sbjct:    1 MCGINNCLEACISSILFHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLPKKPCVIIEMPVQVSNEEDHDLLVDREKYGILSNVPAIPEGFQDVFTLKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSKGCLLFSFYSLISFPVLMNLKMSPGVAKRDFIQLSTKKY 224
[Close]
BLAST of SMED30033550 vs. Planmine SMEST
Match: SMESG000012644.1 (SMESG000012644.1)

HSP 1 Score: 214.157 bits (544), Expect = 2.370e-71
Identity = 110/127 (86.61%), Postives = 111/127 (87.40%), Query Frame = 2 
Query:  344 MPVQVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSK--------------VLMNLKMSPGVAKRDFIQLSTKKY 682
            MPVQV+N EDHDLLVDREKYGILSNVPAIPEGFQDVF LKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSK              VLMNLKMSPGVAKRDFIQLSTKKY
Sbjct:    1 MPVQVSNEEDHDLLVDREKYGILSNVPAIPEGFQDVFTLKSVSQNELKRSEYKQAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSKGCLLFSFYSLISFPVLMNLKMSPGVAKRDFIQLSTKKY 127
[Close]
BLAST of SMED30033550 vs. Planmine SMEST
Match: SMESG000067305.1 (SMESG000067305.1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.151e-9
Identity = 47/160 (29.38%), Postives = 73/160 (45.62%), Query Frame = 2 
Query:   53 MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGI-NV--NEITKLLFQDYLLPKKPCVIIEMPVQVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKS 523
            M G + CL    + IL  +G+  +   +L  L+N I +FL  IGEE   ++  NNQ  +N +H K  L+D  + NV  ++ TK+  +  L P        MP+     +   +   RE +      P IP      F +K +S  E K  E KQ +  K+
Sbjct:    1 MNGFDECLLKSFAIILQSNGFSSIESSALSNLKNVITNFLLQIGEETMTLSVLNNQTKTNFIHLKCALLDTPVANVIRSQATKVESKIDLTPTAIKWTENMPLL----DSEAIQETREFFLFPKAAPPIPMQLHKEFTMKPLSIKEKKLKERKQNVNFKT 156
[Close]
BLAST of SMED30033550 vs. Planmine SMEST
Match: SMESG000067310.1 (SMESG000067310.1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.202e-9
Identity = 47/160 (29.38%), Postives = 73/160 (45.62%), Query Frame = 2 
Query:   53 MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFVTESNNQRISNLLHAKAVLIDYGI-NV--NEITKLLFQDYLLPKKPCVIIEMPVQVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYKQAIAHKS 523
            M G + CL    + IL  +G+  +   +L  L+N I +FL  IGEE   ++  NNQ  +N +H K  L+D  + NV  ++ TK+  +  L P        MP+     +   +   RE +      P IP      F +K +S  E K  E KQ +  K+
Sbjct:    1 MNGFDECLLKSFAIILQSNGFSSIESSALSNLKNVITNFLLQIGEETMTLSVLNNQTKTNFIHLKCALLDTPVANVIRSQATKVESKIDLTPTAIKWTENMPLL----DSEAIQETREFFLFPKAAPPIPMQLHKEFTMKPLSIKEKKLKERKQNVNFKT 156
The following BLAST results are available for this feature:
BLAST of SMED30033550 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033550 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052297.11.159e-14692.41SMESG000052297.1[more]
SMESG000012644.12.050e-14691.52SMESG000012644.1[more]
SMESG000012644.12.370e-7186.61SMESG000012644.1[more]
SMESG000067305.14.151e-929.38SMESG000067305.1[more]
SMESG000067310.15.202e-929.38SMESG000067310.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033550 ID=SMED30033550|Name=SMED30033550|organism=Schmidtea mediterranea sexual|type=transcript|length=711bp
CACGACTTTTTAAATTTTAAATCATTAGTTTGATTTTATTTATTTATCAT
TAATGTGTGGCATTAATAATTGTTTGGATGCTTGTATTTCGAGCATTTTA
CTTCATTCTGGTTATGAATTTGTTACTGATTTATCTTTAGAACTATTACG
AAATTTTATTGTAAGCTTTTTGAAAACTATTGGTGAAGAAATGAAATTTG
TTACAGAAAGTAACAATCAAAGAATATCTAATCTTTTACACGCAAAAGCA
GTTTTAATAGATTATGGAATCAATGTAAATGAAATCACTAAGTTGCTGTT
TCAAGATTATTTGTTACCCAAGAAACCTTGTGTTATTATCGAAATGCCTG
TTCAAGTAAACAATGGGGAAGATCATGATTTATTGGTTGACCGTGAAAAA
TATGGTATTCTAAGTAATGTTCCAGCCATACCTGAAGGCTTTCAAGATGT
TTTTCGATTAAAATCTGTATCTCAAAATGAATTGAAAAGATCTGAATACA
AACAAGCGATTGCTCATAAAAGCCAATTAAAATTCACAGCTATGAAAAAG
TTTTTAAAGCCAAGTATAAGCTCACTAAAATGTCTTCAAGACCGCCAGCT
GTCTAGTAAAGTATTAATGAATCTCAAAATGTCTCCTGGTGTGGCTAAAA
GAGATTTTATTCAATTATCAACAAAAAAATATTAGCATATCCTATTGCTC
CATATTAATTA
back to top

protein sequence of SMED30033550-orf-1

>SMED30033550-orf-1 ID=SMED30033550-orf-1|Name=SMED30033550-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp
MCGINNCLDACISSILLHSGYEFVTDLSLELLRNFIVSFLKTIGEEMKFV
TESNNQRISNLLHAKAVLIDYGINVNEITKLLFQDYLLPKKPCVIIEMPV
QVNNGEDHDLLVDREKYGILSNVPAIPEGFQDVFRLKSVSQNELKRSEYK
QAIAHKSQLKFTAMKKFLKPSISSLKCLQDRQLSSKVLMNLKMSPGVAKR
DFIQLSTKKY*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR009072Histone-fold
IPR006565BTP
Vocabulary: molecular function
TermDefinition
GO:0046982protein heterodimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006565Bromodomain associated domainPFAMPF07524Bromo_TPcoord: 6..77
e-value: 4.9E-8
score: 32.9
IPR009072Histone-foldGENE3DG3DSA:1.10.20.10coord: 4..86
e-value: 6.0E-7
score: 31.1