DNA topoisomerase 2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033538 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16Note: Hover over icons to view figure legend
Homology
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1391.71 bits (3601), Expect = 0.000e+0 Identity = 701/1201 (58.37%), Postives = 890/1201 (74.10%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK A ++ E +NQ D S+ PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++ V +Q+ + + +K K ET PS + RRI P+I
Sbjct: 50 YQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1241
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1390.94 bits (3599), Expect = 0.000e+0 Identity = 701/1201 (58.37%), Postives = 890/1201 (74.10%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK A ++ E +NQ D S+ PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++ V +Q+ + + +K K ET PS + RRI P+I
Sbjct: 55 YQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1246
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]) HSP 1 Score: 1382.47 bits (3577), Expect = 0.000e+0 Identity = 685/1154 (59.36%), Postives = 874/1154 (75.74%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL-------LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3575
Y KKTQLEHILLRPD+Y+GS++ + +W++D E + +++T+ PGLYKI+DEILVNA+DNK RD KMSCIR+ I+ N+I IWNNGKGIPVV H+ ++VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+++EY K FKQTW NMG A + + ED+TC+TF PDL +F M LD + +AL RRAYDIA T + V+LNG ++PVK F+ YV++Y K+ LD LKVI+E+ RWEV +T S F Q+SFVNSIAT+KGG HV+++ DQ KL+++ KKK K +V +MW+FV LIENP+FDSQTKENMTL K FGSTC L++KFI CGIVE I W FK Q L+KKCS KH ++K IPKLDDAN AG +NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ TP +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ LK L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ +++++N++R+++G P+ M P Y+NF GTI E+ N+YV++GE+ L+ TT+EI+ELP+R WT +YKE V+E +G +KTP ITDY+EY+TD TV+F+V+M K AEAE VG HK FKLQT LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A+++KL NQARFILEKI+GK+I EN LI L+ YDSDPVKAW+++ +K+ + ++ E E + + DS P F YL M + LT EKKD L + R+EK +ELD ++ KSP +W +DL +E+ V
Sbjct: 34 YQKKTQLEHILLRPDTYIGSVELVTQQMWVYD-EDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKN---HMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSG-PTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAV 1181
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1365.52 bits (3533), Expect = 0.000e+0 Identity = 673/1110 (60.63%), Postives = 842/1110 (75.86%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLT 3443
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK A ++ E +NQ D S+ PDF Y+ M + +LT
Sbjct: 50 YQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLT 1150
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]) HSP 1 Score: 164.081 bits (414), Expect = 5.187e-46 Identity = 76/127 (59.84%), Postives = 103/127 (81.10%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVT 515
Y KKTQLEHILLRPD+Y+GS++ + +W++D E + +++T+ PGLYKI+DEILVNA+DNK RD KMSCIR+ I+ N+I IWNNGKGIPVV H+ ++VP +IFGQLLTS+N++DD+K+VT
Sbjct: 22 YQKKTQLEHILLRPDTYIGSVELVTQQMWVYD-EDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVT 147
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: top-2 (DNA topoisomerase 2 top-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23670]) HSP 1 Score: 1298.49 bits (3359), Expect = 0.000e+0 Identity = 662/1220 (54.26%), Postives = 873/1220 (71.56%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKA-LWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-DKGEEENQED------------IEDSNKP------DFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDL-----EILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIE 3716
Y KK+QLEHILLRPD+Y+GS++ EK +W+++ E++ + ++ I+Y PGLYKIYDEILVNA+DNK RD KM+ I+I IN KN I ++NNGKGIPV H+ V+VP +IFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS +F LETS+++Y +FKQTW KNM +P I EDFT +TF PDL +F M LDD+ L +RRAYD+A + G+ V+LNG++IP+K F+DYV++YT ++ E LK+ YE+ GDRW+VA+ S F QVSFVNSIATTKGG HV+++ DQ K ++ K+K+ ++ +K Q+K +MWVFV CLIENP+FDSQTKE MTL K FGSTC L++KF + GI + + W FK L+KKCSKTK +KLK IPKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFPLRGKLLNVRE +KQ+ +NAE+ +IKI+GLQY +KYE + KTLRYGKLM+M DQDQDGSHIKGL+INFIH WP+L++ NFVE+FITPIVKA K + +SFYS+PE EW+ T +++KYYKGLGTSTSKEAKEYF DM RH I+F+Y+G +D ++ +AFSK K +ERK+WL+ WM + K R++ G+ E YLY K T+++TF++F+N+EL+LFSN DNERSIPCL+DGFKPGQRKVLFAC KR +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGSNNINLL+P GQFGTRL GGKDSAS RYI TQLS +T+ +F D+ VL+ L ++NQ IEPEWY PIIPMVL+NG GIGTGWS+ IPN+NP++++ N+KR+I G + PWY+NF G I+++ +++ GE+ LD T+EITELPI+ WT YKE V+E +DK I DYKEY+TD TV+F+V+++P K E E H+ FKLQ + + M+LFD G +R Y + I + F+ R E Y++RK YLLG+L AQ+ +L NQARFIL KIN +++ EN K A ++D L+ K+D+DPVK W K +KL + + GE E ED I + K D+ YL M ++ L+ E+K+ L+KE +EK+ E+ +I K+ Q +W +DL E+ ++E +QD S + G + E PSK +RIEP ++
Sbjct: 67 YQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQ-FNNEGEPLKIAYEQVGDRWQVALALS-EKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTK--TSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKW-KEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDAS-IKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLD 1280
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 805.438 bits (2079), Expect = 0.000e+0 Identity = 450/1135 (39.65%), Postives = 685/1135 (60.35%), Query Frame = 3
Query: 132 RYLKKTQLEHILLRPDSYVGSIK-PEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKE---DFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAAC-TNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQ--------VSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXM--NMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVA-KCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKR-NLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMING-GTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQL-------DGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMN---PAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTA 3446
+Y KK+ EH+LLRPD+Y+G + E++ +W+ DSE M+ K++TY PGL KI+DEILVNA+DNK RD M+ + + ++ I +WNNG G+PV +H ++VPT++FG L TS+N++D + + GGRNGYGAKLCNIFS EFI+ET + + F+Q W NM ++ + E D+T V F PDL+RF + L D+ I L RR +++AA + VYLNGQ+ V F+DYV+++ + +++ RW V + N+ F + VSFVN+I T KGG+HV+++ D K++ I K + S V + N+ +F+ CLIENPSF+SQTKE +T AK+FGS K ++ A + G++E I + N + + ++DI KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W V G +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +RSIP L+DG KP QRK+L+ L + E+KV+QLAG+VA +YHHGE SL+ TII + Q F GS+N+ LL P GQFGTR GG D+AS RYI T L+ T+L+F D+ +L+ +++ +EP W PI+P++LING +GIGTGWS+KI N NP D+ID ++R I+ T ++ P+Y F G + V K++ +G+I + ++EI ELPI +WT+ YKE + +I + P + ++ E +T+K V F + ++ +++ + +FKL+T LT N +LFD G ++++ N EI FF+VR ++Y KR A+ ++NQ FI NG + ++ L +KL + DP+ K +S K ++GYL +M + LT+
Sbjct: 46 KYEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFNDSS--------SLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD----KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDAKKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRSIPSLIDGLKPSQRKILWTLLNNMDESTEIKVSQLAGAVAHRQSYHHGEESLVRTIIRMGQTFCGSSNLPLLQPIGQFGTRHEGGNDAASARYIFTALAPTTRLLFPQADDDLLQKNVEEGMVVEPTWLCPIVPLILINGTEGIGTGWSTKIANRNPIDIIDMIRRKIDSISTEYEIPPFYEEFRGKLEVVTPTKFISSGKIQLIRPERKNASTFSIEIVELPIGIWTSKYKEKLSKIV----ETLP--VLEFSERHTEKRVHFRITLDRKKSSRFLQKSNSDLLNYFKLRTSLT-ENRVLFDRNGELKEFGNISEIAAEFFEVRRDLYEKRLKIQKEECEAKLIYVENQLNFIEMVTNGTIEIRSMGRNQLEEKLQEMGFRVDPMATIAK--------------------NSKKANYGYLLEMPVSRLTS 1124
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: cin-4 (DNA topoisomerase-like protein cin-4 [Source:UniProtKB/Swiss-Prot;Acc:G5ECQ8]) HSP 1 Score: 768.074 bits (1982), Expect = 0.000e+0 Identity = 386/755 (51.13%), Postives = 524/755 (69.40%), Query Frame = 3
Query: 1362 IPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEE-------------ENQEDIEDSNKP------DFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEI 3566
IPKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFP R KLLNV + V Q+ ++AE+ IIKI+GLQY++KYE + KTLRYGKL+IM + D DGS KGLL+NF H WP L K +FVE FITPI KA + + +SFYS+PE EW+ T + +KYY GLGT TSKEAK+ FSDM RH I+F+Y+G +D K++ +AFSK K + R ++L M+D +R++ G+ E LY K T+++T ++F N E++ + N SIPCL+DG KPGQRKVLFAC KR +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGS+NINLL+P GQFGTRL GGKDSAS R I QLS++T+ +F D+ VL+ L ++NQ IEPEWY PIIPMVL+NG G GTGWS+ IPN+NP++++ N+KR+I G + PWY+NF G I+++ +++ GE++ LD T+EITELPI+ WT YKE V+E +DK I DYKEY+TD TV+F+V+++P K E E H+ FKLQ + + M+LFD G +R Y + I + F+ R E Y++RK YLLG+L AQ+ +L NQARFIL IN K++ EN K ++D L+ K+D+DPVK W++ +KL + + E E EDI + K D+ YL + ++ L+ E+K+ L+KE +EK+ E+ ++ K+ Q +W+ DL+ E+
Sbjct: 40 IPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPFRRKLLNVCDLNVNQIADSAEVNAIIKILGLQYTKKYETEDDFKTLRYGKLLIMANHDSDGSQFKGLLLNFFHRFWPALFKRDFVEDFITPIAKATEGKEEVSFYSLPEYSEWRMNTDNWKSYTIKYYNGLGTLTSKEAKKCFSDMVRHRIRFKYNGADDDKAVNMAFSKKKIEARTDYLMKLMQDKNQRKQQGLAEECLYNKETRFVTLKDFFNYEIVC---SWNLHSIPCLVDGLKPGQRKVLFACFKRANKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSHNINLLLPIGQFGTRLQGGKDSASARSIFAQLSQVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGTGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVSVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGWLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILATINNKIVLENKKKTAIVDVLIKMKFDADPVKKWKED-QKLKELRESGEIELDEDDLAAVAVEEGEDISSAAKAVETKLSDYDYLVGLALIKLSEEEKNKLIKESEEKMAEVRVLEKKTWQDLWITDLDNFMSEL 790
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 537.339 bits (1383), Expect = 4.747e-173 Identity = 293/705 (41.56%), Postives = 444/705 (62.98%), Query Frame = 3
Query: 132 RYLKKTQLEHILLRPDSYVGSIK-PEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKE---DFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAAC-TNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQ--------VSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXM--NMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVA-KCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2192
+Y KK+ EH+LLRPD+Y+G + E++ +W+ DSE M+ K++TY PGL KI+DEILVNA+DNK RD M+ + + ++ I +WNNG G+PV +H ++VPT++FG L TS+N++D + + GGRNGYGAKLCNIFS EFI+ET + + F+Q W NM ++ + E D+T V F PDL+RF + L D+ I L RR +++AA + VYLNGQ+ V F+DYV+++ + +++ RW V + N+ F + VSFVN+I T KGG+HV+++ D K++ I K + S V + N+ +F+ CLIENPSF+SQTKE +T AK+FGS K ++ A + G++E I + N + + ++DI KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W V G +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +R+
Sbjct: 46 KYEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFNDSS--------SLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD----KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDAKKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 537.339 bits (1383), Expect = 4.747e-173 Identity = 293/705 (41.56%), Postives = 444/705 (62.98%), Query Frame = 3
Query: 132 RYLKKTQLEHILLRPDSYVGSIK-PEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKE---DFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAAC-TNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQ--------VSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXM--NMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVA-KCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2192
+Y KK+ EH+LLRPD+Y+G + E++ +W+ DSE M+ K++TY PGL KI+DEILVNA+DNK RD M+ + + ++ I +WNNG G+PV +H ++VPT++FG L TS+N++D + + GGRNGYGAKLCNIFS EFI+ET + + F+Q W NM ++ + E D+T V F PDL+RF + L D+ I L RR +++AA + VYLNGQ+ V F+DYV+++ + +++ RW V + N+ F + VSFVN+I T KGG+HV+++ D K++ I K + S V + N+ +F+ CLIENPSF+SQTKE +T AK+FGS K ++ A + G++E I + N + + ++DI KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W V G +W++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +R+
Sbjct: 46 KYEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFNDSS--------SLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD----KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDAKKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWADVEEGG-KWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0332320) HSP 1 Score: 1319.29 bits (3413), Expect = 0.000e+0 Identity = 660/1169 (56.46%), Postives = 856/1169 (73.22%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-----------------DKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3569
Y KK+QLEHILLRPDSY+GS++ ++ +W++D+ +N MV+K+I++ PGLYKI+DEILVNA+DNK RD+ M+ I+IDI+ +NM+ +WNNG+GIPV MH++ ++VPTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS F +ET+ +EY KSFKQTW NMG ASD I D+T +TF PDL +F M LD++ +AL SRRAYD+AA + G+ V+LNG ++ V+NFKDY++L+ KN D + +K+++E +RWEVA S + F QVSFVNSIAT KGG HV+H+ D +LLE+ KKK K +V ++WVFV CLIENP+FDSQTKENMTL K FGS C L++KFI+ ++K GIVE + WA FK Q K K +K+K IPKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA KQL+ENAEI N+ KIIGLQY +KY + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+ F+E+FITPIVKA K N+ LSFYS+PE EW+ T + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G D +SIV+AFSK + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN +E+KVAQL+GSVAE+SAYHHGE SL TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H D+ +L +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING P MHPWY+NF G + V + +Y+ G I L G LEI+ELP+ VWT +YKE+V+E +G +K I++Y+EY+TD TVRF++ P ++ AE GF++ FKL T L+ + M FD+ +R++ I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK K++ EN + + D+L+ Y DPVK WQ+ ++ A K ++E + D E + K F YL M + LT EKK+ LLK+RD KL EL+ +R K+P+ +WLDDL+ LE ++N
Sbjct: 14 YQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRN---HLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0081287) HSP 1 Score: 1319.29 bits (3413), Expect = 0.000e+0 Identity = 660/1169 (56.46%), Postives = 856/1169 (73.22%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAI-----------------DKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3569
Y KK+QLEHILLRPDSY+GS++ ++ +W++D+ +N MV+K+I++ PGLYKI+DEILVNA+DNK RD+ M+ I+IDI+ +NM+ +WNNG+GIPV MH++ ++VPTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS F +ET+ +EY KSFKQTW NMG ASD I D+T +TF PDL +F M LD++ +AL SRRAYD+AA + G+ V+LNG ++ V+NFKDY++L+ KN D + +K+++E +RWEVA S + F QVSFVNSIAT KGG HV+H+ D +LLE+ KKK K +V ++WVFV CLIENP+FDSQTKENMTL K FGS C L++KFI+ ++K GIVE + WA FK Q K K +K+K IPKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA KQL+ENAEI N+ KIIGLQY +KY + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+ F+E+FITPIVKA K N+ LSFYS+PE EW+ T + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G D +SIV+AFSK + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN +E+KVAQL+GSVAE+SAYHHGE SL TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H D+ +L +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING P MHPWY+NF G + V + +Y+ G I L G LEI+ELP+ VWT +YKE+V+E +G +K I++Y+EY+TD TVRF++ P ++ AE GF++ FKL T L+ + M FD+ +R++ I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK K++ EN + + D+L+ Y DPVK WQ+ ++ A K ++E + D E + K F YL M + LT EKK+ LLK+RD KL EL+ +R K+P+ +WLDDL+ LE ++N
Sbjct: 14 YQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRN---HLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 1380.93 bits (3573), Expect = 0.000e+0 Identity = 698/1198 (58.26%), Postives = 893/1198 (74.54%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKG---EEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP--GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD E M ++I++ PGLYKI+DEILVNA+DNK RD+ M+ I+I I+ N I +WNNGKGIPVV H+D ++VP +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KEY +SFKQTWT+NMG +++ I EDFTCVTF PDL +FNM LD + +AL +RRAYD+A G+ V LNG+++PV +F+ YV+ Y K+ LD LKV+ E +RWEV +T S F Q+SFVNSIATTKGG H+++I DQ KL+E+ KKK K+ +V ++WVFV LIENP+FDSQTKENMTL K FGS CAL++KFI CGIVE I W FK Q L+KKCS KH+K+K IPKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYG++MIMTDQDQDGSHIKGLLINF H WP+LLKH F+E+FITPIVKA KN Q +SFYSIPE EW+K T W +KYYKGLGTSTSKEAKEYFSDMERH I F+Y+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F D ++LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF GTI E+ +N+Y+V+GE++ LD T+EI+ELP+R WT +YKESV+E G DKTP I+DYKEY+TD TV+F+V M K A+AEA G HK FKLQ+ LT ++M+LFD G +++Y + EIL+ FF++RL Y RK++L+G L A+++KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K+ EK L + G + ++ D + P+F Y+ M + LT EK + LLK+RD K EL+ +++K+ + +W +DL + +E++ V Q+ G+ V +VK K ET PS F RR+EP+I
Sbjct: 54 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDNEKKVTGGRNGYGAKLCNIFSLKFTVETACKEYKRSFKQTWTENMGKSNEAKIKYFDGEDFTCVTFQPDLAKFNMEKLDKDIVALLTRRAYDVAGSCRGVKVILNGKKLPVNSFRSYVDFYVKDKLDETGVALKVVNETVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYIVDQIVGKLIEVVKKKNKAGVSVKPFQVKN---HLWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKILGLQYKKSYDDPESLKSLRYGRIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEVSFYSIPEFEEWKKHTESYKTWHIKYYKGLGTSTSKEAKEYFSDMERHRIMFKYNGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTSTRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCVKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSSLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGTIHELGQNQYLVSGEVSVLDKNTIEISELPVRTWTQAYKESVLEPMLQGTDKTPPLISDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQEILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKLSIENKSKRDLIRMLVQRGYESDPVAAWNKAQEKSLEEEDGGGNDSDSSVDSGSFSGPNFNYILNMPLWCLTKEKVEELLKQRDLKKAELNELQSKTSENLWREDLAVFIEELDRVEEQEKESVNLGKAVK-LVKGKVGKPKVKKMQLEETLPSPFGRRVEPQI 1245
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1355.12 bits (3506), Expect = 0.000e+0 Identity = 685/1195 (57.32%), Postives = 885/1195 (74.06%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD + NC + IT+ PGLYKI+DEILVNA+DNK RD+ M+CI+++I+ N I +WNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ +E + FKQTW NMG A + I +D+TC+TF PDL +F M LD +T+AL +RRAYDIA T G+ V LNG+++P+ NF+ YV+LY K+ +D L V++E +RWEV +T S F QVSFVNSIATTKGG H +++ DQ KL+E+ KKK K+ +V +MW+FV CLIENPSFDSQTKENMTL K+FGS C+L+DKF+ Q CGIVE I W FK QA L+KKCS KHTK+K +PKLDDAN AG KNS CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y PESLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+KA Q +SFYSIPE EW++ W++KYYKGLGTSTSKEAKEYFSDM RH I F+YSG D ++I LAFSK +ERKEWLT++M + ++RRE + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPC++DG KPGQRKV+F C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LK DDN +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG P+ M P Y+ F GTI E+ +N+YV NGE+ +D TT+EITELP++ WT +YKE+V+E+ +G +K PA ITDYKEY+TD TVRF+V+M + EAEA G HK FKLQ LT ++M+LFD G ++KY + +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVKAW+ + EK D +++ +E + S PD+ YL M + LT EKKD L K+RD K+ EL+ ++ K+P +W +DL +E+ V ++ + + K K +K+ ET P+ RR+ P+I
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDTGMNC---RDITFVPGLYKIFDEILVNAADNKQRDKTMNCIKVNIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSLKFTVETACRESKRCFKQTWFDNMGRAGESKIKPFDGDDYTCITFQPDLAKFKMQALDKDTVALLTRRAYDIAGSTKGVRVLLNGKRLPINNFRTYVDLYVKDKVDETGSPLTVVHEMVNERWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKKNKAGVAVKPFQVKN---HMWLFVNCLIENPSFDSQTKENMTLQQKNFGSACSLSDKFVKQANGCGIVESIMNWVKFKAQAQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSNSCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKILGLQYKKNYSDPESLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKQEISFYSIPEFTEWKEKQSSIKSWKIKYYKGLGTSTSKEAKEYFSDMTRHRIPFKYSGPADDEAITLAFSKKMIEERKEWLTSFMINRRQRREHNLPEEYLYGQETKSLSYHDFINKELVLFSNSDNERSIPCMVDGLKPGQRKVVFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKEEEEDAEDDDEKEKEDTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKEKESQNAM---PLVKGKVGKPKVVKVKNETMPTPQGRRVIPRI 1216
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 416.387 bits (1069), Expect = 7.632e-127 Identity = 226/459 (49.24%), Postives = 316/459 (68.85%), Query Frame = 3
Query: 2337 SLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LK DDN +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG P+ M P Y+ F GTI E+ +N+YV NGE+ +D TT+EITELP++ WT +YKE+V+E+ +G +K PA ITDYKEY+TD TVRF+V+M + EAEA G HK FKLQ LT ++M+LFD G ++KY + +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVKAW+ + EK D +++ +E + S PD+ YL M + LT EKKD L K+RD K+ EL+ ++ K+P +W +DL +E+ V ++ + + K K +K+ ET P+ RR+ P+I
Sbjct: 2 SLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKEEEEDAEDDDEKEKEDTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKEKESQNAM---PLVKGKVGKPKVVKVKNETMPTPQGRRVIPRI 456
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 119.398 bits (298), Expect = 1.157e-30 Identity = 54/92 (58.70%), Postives = 73/92 (79.35%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKG 410
Y KKTQLEHILLRPD+Y+GS++P + +W+FD E+ M ++I++ PGLYKI+DEILVNA+DNK RD+ M+ I+I I+ N I +WNNGKG
Sbjct: 49 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFD-EEIGMNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKG 139
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1405.96 bits (3638), Expect = 0.000e+0 Identity = 707/1195 (59.16%), Postives = 884/1195 (73.97%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD ++ NC + +T+ PGLYKI+DEILVNA+DNK RD MSCI+I IN N + +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFSN+F +ET+ +EY K FKQTWT NM A+D I ED+TC+TF PDL +F M LD + +AL SRRAYD+A + G+ V+LNG+++P+ F+ YV+LY KN LD LKV++E DRWE+ +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K +V +MWVFV LIENP+FDSQTKENMTL +K FGS C L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ T W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEA G HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W+++ EK A + EEE + ++ + PDF YL M + L+ EKKD L K+RD K EL ++ KSP +W +DL +E++ V ++ E VVK + E PS RR+ PK+
Sbjct: 31 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVKPFQVKN---HMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEVNTAG-PDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1402.88 bits (3630), Expect = 0.000e+0 Identity = 707/1195 (59.16%), Postives = 885/1195 (74.06%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD ++ NC + +T+ PGLYKI+DEILVNA+DNK RD MSCI+I IN N + +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFSN+F +ET+ +EY K FKQTWT NM A+D I ED+TC+TF PDL +F M LD + +AL SRRAYD+A + G+ V+LNG+++P+ F+ YV+LY KN LD LKV++E DRWE+ +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K +V +MWVFV LIENP+FDSQTKENMTL +K FGS C L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ T W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEA G HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W+++ EK A + EEE + ++ ++ PDF YL M + L+ EKKD L K+RD K EL ++ KSP +W +DL +E++ V ++ E VVK + E PS RR+ PK+
Sbjct: 31 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVKPFQVKN---HMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEV-NTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1402.5 bits (3629), Expect = 0.000e+0 Identity = 707/1195 (59.16%), Postives = 884/1195 (73.97%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD ++ NC + +T+ PGLYKI+DEILVNA+DNK RD MSCI+I IN N + +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFSN+F +ET+ +EY K FKQTWT NM A+D I ED+TC+TF PDL +F M LD + +AL SRRAYD+A + G+ V+LNG+++P+ F+ YV+LY KN LD LKV++E DRWE+ +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K +V +MWVFV LIENP+FDSQTKENMTL +K FGS C L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ T W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEA G HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W+++ EK A + EEE + ++ + PDF YL M + L+ EKKD L K+RD K EL ++ KSP +W +DL +E++ V ++ E VVK + E PS RR+ PK+
Sbjct: 31 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVKPFQVKN---HMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEVNTAG-PDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1402.5 bits (3629), Expect = 0.000e+0 Identity = 707/1195 (59.16%), Postives = 884/1195 (73.97%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD ++ NC + +T+ PGLYKI+DEILVNA+DNK RD MSCI+I IN N + +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFSN+F +ET+ +EY K FKQTWT NM A+D I ED+TC+TF PDL +F M LD + +AL SRRAYD+A + G+ V+LNG+++P+ F+ YV+LY KN LD LKV++E DRWE+ +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K +V +MWVFV LIENP+FDSQTKENMTL +K FGS C L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ T W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEA G HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W+++ EK A + EEE + ++ + PDF YL M + L+ EKKD L K+RD K EL ++ KSP +W +DL +E++ V ++ E VVK + E PS RR+ PK+
Sbjct: 31 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVKPFQVKN---HMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKEEAGELLEEEEETEVNTAG-PDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1217
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1399.03 bits (3620), Expect = 0.000e+0 Identity = 704/1195 (58.91%), Postives = 880/1195 (73.64%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD ++ NC + +T+ PGLYKI+DEILVNA+DNK RD MSCI+I IN N + +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFSN+F +ET+ +EY K FKQTWT NM A+D I ED+TC+TF PDL +F M LD + +AL SRRAYD+A + G+ V+LNG+++P+ F+ YV+LY KN LD LKV++E DRWE+ +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K +V +MWVFV LIENP+FDSQTKENMTL +K FGS C L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ T W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK + DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEA G HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W+++ EK+ E ++ + PDF YL M + L+ EKKD L K+RD K EL ++ KSP +W +DL +E++ V ++ E VVK + E PS RR+ PK+
Sbjct: 31 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVKPFQVKN---HMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQTQEKVFIC----VEFRDRSLLPSGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVKGKSGRPQVKKMQLQEIMPSPHGRRVVPKV 1214
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2a (topoisomerase (DNA) II alpha [Source:MGI Symbol;Acc:MGI:98790]) HSP 1 Score: 1392.87 bits (3604), Expect = 0.000e+0 Identity = 689/1151 (59.86%), Postives = 875/1151 (76.02%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL----LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3575
Y KKTQLEHILLRPD+Y+GS++ + +W++D E + +++T+ PGLYKI+DEILVNA+DNK RD KMSCIR+ I+ N+I IWNNGKGIPVV H+ ++VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+++EY K FKQTW NMG A D + ED+TC+TF PDL +F M LD + +AL RRAYDIA T + V+LNG +PVK F+ YV+LY K+ +D LKVI+E+ RWEV +T S F Q+SFVNSIAT+KGG HV+++ DQ KL+++ KKK K +V +MW+FV LIENP+FDSQTKENMTL AK FGSTC L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ TP +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ L+ L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ LD TT+EI+ELPIR WT +YKE V+E +G +KTP+ ITDY+EY+TD TV+F+++M K AEAE VG HK FKLQ+ LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A++SKL NQARFILEKI+GK++ EN LI L+ YDSDPVKAW+++ +K+ + E + D P F YL M + LT EKKD L K+R+EK +EL+ ++ KSP +W +DL + +E+ VV
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVELVTQQMWVYD-EDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKN---HMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 1383.62 bits (3580), Expect = 0.000e+0 Identity = 697/1199 (58.13%), Postives = 886/1199 (73.89%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE-PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK + + ++ + DS + PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++ V Q+ + + +K K ET PS + RRI P+I
Sbjct: 43 YQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEI 1234
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 184.111 bits (466), Expect = 4.773e-52 Identity = 84/156 (53.85%), Postives = 116/156 (74.36%), Query Frame = 3
Query: 2598 INGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDE 3065
ING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M +E
Sbjct: 2 INGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMAAEEE 157
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 66.6254 bits (161), Expect = 8.645e-11 Identity = 49/124 (39.52%), Postives = 76/124 (61.29%), Query Frame = 3
Query: 3207 RFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEIN 3569
RFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK + + ++ + DS + PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++
Sbjct: 1 RFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELD 124
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P41515|TOP2A_CRIGR (DNA topoisomerase 2-alpha OS=Cricetulus griseus OX=10029 GN=TOP2A PE=1 SV=1) HSP 1 Score: 1394.79 bits (3609), Expect = 0.000e+0 Identity = 691/1150 (60.09%), Postives = 881/1150 (76.61%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL---LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3575
Y KKTQLEHILLRPD+Y+GS++ + +W++D E + +++T+ PGLYKI+DEILVNA+DNK RD KMSCIR+ I+ N+I IWNNGKGIPVV H+ ++VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS F +ET++KEY K FKQTW NMG A D + ED+TC+TF PDL +F M LD + +AL RRAYDIA T + V+LNG ++PVK F+ YV++Y K+ LD LKV++E+ RWEV +T S F Q+SFVNSIAT+KGG HV+++ DQ KL+++ KKK K +V +MW+FV LIENP+FDSQTKENMTL AK FGSTC L++KFI CGIVE I W FK Q L+KKCS KH ++K IPKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q L+FYS+PE EW+ TP +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLT++MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS LT+L+F D+ LK L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ L+ TT+EI+ELPIR WT +YKE V+E +G +KTP ITDY+EY+TD TV+F+++M K AEAE VG HK FKLQT LT ++M+LFD G ++KY+ ++IL+ FF++RL+ Y RK +LLG+L A+++KL NQARFILEKI+GK+I EN LI L+ YDSDPVKAW+++ +K+ ++ + EN + + +S P F YL M + LT EKKD L K+R+EK +EL+ ++NKSP +W +DL + +E+ VV
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVELVTQQMWVYD-EDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTRFTVETASKEYKKMFKQTWMDNMGRAGDMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNALKVVHEQVNPRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKN---HMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILKDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENSDSVAESG-PTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKNKSPSDLWKEDLAVFIEELEVV 1176
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q01320|TOP2A_MOUSE (DNA topoisomerase 2-alpha OS=Mus musculus OX=10090 GN=Top2a PE=1 SV=2) HSP 1 Score: 1392.87 bits (3604), Expect = 0.000e+0 Identity = 689/1151 (59.86%), Postives = 875/1151 (76.02%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKL----LAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3575
Y KKTQLEHILLRPD+Y+GS++ + +W++D E + +++T+ PGLYKI+DEILVNA+DNK RD KMSCIR+ I+ N+I IWNNGKGIPVV H+ ++VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+++EY K FKQTW NMG A D + ED+TC+TF PDL +F M LD + +AL RRAYDIA T + V+LNG +PVK F+ YV+LY K+ +D LKVI+E+ RWEV +T S F Q+SFVNSIAT+KGG HV+++ DQ KL+++ KKK K +V +MW+FV LIENP+FDSQTKENMTL AK FGSTC L++KFI CGIVE I W FK Q L+KKCS KHTK+K IPKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ TP +W++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ L+ L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ LD TT+EI+ELPIR WT +YKE V+E +G +KTP+ ITDY+EY+TD TV+F+++M K AEAE VG HK FKLQ+ LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A++SKL NQARFILEKI+GK++ EN LI L+ YDSDPVKAW+++ +K+ + E + D P F YL M + LT EKKD L K+R+EK +EL+ ++ KSP +W +DL + +E+ VV
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVELVTQQMWVYD-EDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKN---HMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q02880|TOP2B_HUMAN (DNA topoisomerase 2-beta OS=Homo sapiens OX=9606 GN=TOP2B PE=1 SV=3) HSP 1 Score: 1390.94 bits (3599), Expect = 0.000e+0 Identity = 701/1201 (58.37%), Postives = 890/1201 (74.10%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK-----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEP-GEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK A ++ E +NQ D S+ PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++ V +Q+ + + +K K ET PS + RRI P+I
Sbjct: 55 YQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEK--AAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEI 1246
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|O42130|TOP2A_CHICK (DNA topoisomerase 2-alpha OS=Gallus gallus OX=9031 GN=TOP2A PE=2 SV=2) HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0 Identity = 716/1267 (56.51%), Postives = 913/1267 (72.06%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE-----PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSE 3914
Y KKTQLEHILLRPD+Y+GS++ + +W+FD + NC + +T+ PGLYKI+DEILVNA+DNK RD+ MSCI++ I+ N I +WNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ +EY K FKQTWT NMG A + + ED+TCVTF PDL +F M+ LD + +AL SRRAYDIA T + V+LNG+++PVK F+ YV+LY K+ +D LKVI+E+ RWEV +T S F QVSFVNSIATTKGG HV+++ DQ KL+++ KKK K+ +V +MW+FV LIENP+FDSQTKENMTL AK FGSTC L++KFI CGIVE I W FK Q L+KKCS KHTK+K +PKLDDAN AG+KNS+ CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE ESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+K KN + + FYSIPE EW+ T N W++KYYKGLGTSTSKEAKEYF+DM RH I F+YSG ED +I LAFSK K +ERKEWLTN+MED ++R G+ E YLYGK T Y+T+ +FIN+EL+LFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+K AQLAGSVAE+S+YHHGEASLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ VL+ L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++ ++N++ +++G P+ M P Y+NF GTI E+ N+YV++GE++ LD TT+EITELP+R WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V+M+ K AEAEAVG HK FKLQT LT ++M+LFD G ++KY + +IL+ FF++RL Y RK +L+G+L A+++KL NQARFILEKI+GK++ EN LI L+ Y+SDPVKAW++ K +E+ E+ + PDF YL M + LT EKKD L K+RD K KEL+ +++KSP +W +DL +E++ V + + G+ + K G K AE PS R+ P++ E + I+ + E ++ + N+S + E +L QRLA+ +E
Sbjct: 35 YQKKTQLEHILLRPDTYIGSVETVTQQMWVFDEDVGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVTIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACREYKKLFKQTWTDNMGKAGEMTLKHFDGEDYTCVTFQPDLSKFKMTILDKDIVALMSRRAYDIAGSTKDVKVFLNGKRLPVKGFRSYVDLYLKDKVDETGNALKVIHEEVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHVDYVADQIVTKLIDVVKKKNKNGVGVKPFQVKN---HMWIFVNSLIENPTFDSQTKENMTLQAKSFGSTCKLSEKFIKGAVGCGIVESILNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGSKNSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDRESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSKNKEEIPFYSIPEFEEWKSSTQNYNSWKIKYYKGLGTSTSKEAKEYFADMARHRIGFKYSGPEDDAAITLAFSKKKVEERKEWLTNFMEDRRQRNVHGLPEDYLYGKDTNYLTYNDFINKELVLFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKGAQLAGSVAEMSSYHHGEASLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPVDDNVLRFLYDDNQRVEPEWYMPIIPMVLINGAEGIGTGWSCKIPNFDIRETVNNIRCLLDGKEPLPMLPSYKNFKGTIDELGPNQYVISGEVSILDSTTIEITELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKMSEEKLAEAEAVGLHKVFKLQTNLTCNSMVLFDHVGFLKKYESPQDILKEFFELRLRYYGLRKEWLIGMLGAESAKLNNQARFILEKIDGKIVIENKPKKELIQVLIQRGYESDPVKAWKELQNKEEEE---GDESGEESAAATGPDFNYLLNMPLWYLTKEKKDELCKQRDNKDKELEDLKHKSPSDLWKEDLAAFVEELDAVEAKQMQDEMAGITGKPLKVKGGKQGGKQKVTKAQLAEVMPSPHGIRVVPRVTAEMKAEAEKR----------------IKKKIKSEKNESDEKQEGNSSGDKEPSSLKQRLAQKRKAE 1275
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q64511|TOP2B_MOUSE (DNA topoisomerase 2-beta OS=Mus musculus OX=10090 GN=Top2b PE=1 SV=2) HSP 1 Score: 1383.62 bits (3580), Expect = 0.000e+0 Identity = 697/1199 (58.13%), Postives = 886/1199 (73.89%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSE--KNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDS---NKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE-PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W++D + NC +++T+ PGLYKI+DEILVNA+DNK RD+ M+CI++ I+ N+I IWNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+ I ED+TC+TF PDL +F M LD + +AL +RRAYD+A G+ V NG+++PV F+ YV+LY K+ LD LKVI+E +RW+V +T S F Q+SFVNSIATTKGG HV+++ DQ KL+E+ KKK K+ +V ++WVF+ CLIENP+FDSQTKENMTL K FGS C L++KF + CGIVE I W FK Q L+KKCS K++K+K IPKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+K W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELP+R WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEA G HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW+++ EK + + ++ + DS + PDF Y+ M + +LT EK + L+K+RD K +E++ ++ KSP +W +DL +E++ V Q+ + + +K K ET PS + RRI P+I
Sbjct: 43 YQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNC---REVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKN---HIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEI 1234
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A094ZNL1 (DNA topoisomerase 2 OS=Schistosoma haematobium OX=6185 GN=MS3_03959 PE=3 SV=1) HSP 1 Score: 1450.26 bits (3753), Expect = 0.000e+0 Identity = 760/1353 (56.17%), Postives = 982/1353 (72.58%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEEN------QEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTV---------VXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGD-NNSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMS 4115
Y KKTQLEHILLRPDSY+GS + + +W+ D + MV + IT++PGLYKI+DEILVNA+DNK+RD MSCI+IDIN +NM+RIWN+GKGIPVV H+ N +VPT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +FI+ETS+KEY KSF+Q W +NM +DP I + + +DFTC+TFYPDL RF M+ L+ +T++LF+RRAYD+AA NG+ V+LNG+++P+K+F DYV L+ K D + +KV+YE RW+VA+ S N F QVSFVNSIATTKGGTH+N+++DQ KL+EI KKK S K V++K Q+K +MW+FV CLIENP+FDSQTKENMTL K FGSTC L++KFI Q +K GIVE + W FK +DK+C K+KH+KLK IPKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H WPNLLKHN VE+FITPIVK FK Q SFYS+PE EWQK TP + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED SI LAF K+K +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N ++I+NL RM++G P +M P +R FTGTI ++ N+YV GE+ LD TLEITELPIRVWT +YKESV+E +G++K PA ITDYKEY+TD TVRF+V+M+P K EAE+ G HKFFKLQT ++ +M+ FD G ++ Y N + I+ F+++RL Y KRK YL G+LSA+A KL+NQARF+LEKI ++ EN LI L A YDSDPVKAW++S++K A+ + EE Q + ++ +PD+ Y+ M + +LT E+KD LL +RD K KEL I+++KSP +W +DL+ LE+E V + ++ + V K+ K+A ET P RRI P ++ + +KK+ +K+ K + + ++N + EA D N NDE LAQRL + ++ K +G + T++ +L + +SP GN +++ S + S +D GDS ++Q + ++ +S
Sbjct: 36 YQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVSRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVENMTKTTDPKILADKGDDFTCITFYPDLARFGMNELEQDTVSLFTRRAYDMAATCNGVKVFLNGKRLPIKSFMDYVNLFIKPNKDDGDS-VKVVYEAVNPRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMEIVKKK--SGKSGVQIKPFQIKNHMWLFVNCLIENPTFDSQTKENMTLQTKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESVDTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEYSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGTEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGNNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVVKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEMVIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAAVQEQEESRTDEGAPQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAVEKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADMMKKVEGNKKKRNKTENDLNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGASEKTDVQKTATTRGRGKGRTVPTSRPKLLKPSSPNISPIKRRNVGGNAKRKRSSRMPWESGSDTGDS-ESQVSIDSDNDHIS 1374
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A3Q0KH18 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 PE=3 SV=1) HSP 1 Score: 1446.03 bits (3742), Expect = 0.000e+0 Identity = 759/1338 (56.73%), Postives = 974/1338 (72.80%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEE------NQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---------DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDS 4070
Y KKTQLEHILLRPDSY+GS + + +W+ D + MV + IT++PGLYKI+DEILVNA+DNK+RD MSCI+IDIN +NM+RIWN+GKGIPVV H+ N +VPT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +FI+ETS+KEY KSF+Q W NM SDP I + + EDFTC+TFYPDL RF M+ L+ +T++LF+RRAYD+AA NGI V+LNG+++P+KNF DYV L+ K D + + +KV+YE RW+VA+ S N F QVSFVNSIATTKGGTH+N+++DQ KL++I KKK S K V++K Q+K +MW+FV CLIENP+FDSQTKENMTL K FGSTC L++KFI Q +K GIVE + W FK +DK+C K+KH+KLK IPKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H WPNLLKHN VE+FITPIVK FK Q SFYS+PE EWQK TP + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED SI LAF K+K +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N ++I+NL RM++G P +M P +R FTGTI ++ N+YV GE+ LD TLEITELPIRVWT +YKESV+E +G++K PA ITDYKEY+TD TVRF+++M+P K EAE+ G HKFFKLQT ++ +M+ FD G ++ Y N I I+ F+++RL Y KRK YL G+LSA+A KL+NQARF+LEKI ++ EN LI L A YDSDPVKAW++S++K A+ + EE +Q + ++ +PD+ Y+ M + +LT E+KD LL +RD K KEL I+++KSP +W +DL+ LE+E V + + ++ + + K+ K+A ET P RRI P ++ + +KK+ +K+ K + + ++N + EA D N NDE LAQRL + ++ K +G + T++ + + SP GN +++ S + S +D GDS
Sbjct: 36 YQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKK--SGKSGVQIKPFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADIMKKVEGNKKKRNKTENDPNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGVSEKTDIQKTATTRGRGKGRTVPTSRPKLPKPSSPNNSPIKRKNMGGNAKRKRSSRMPWESGSDTGDS 1360
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A0X3PUD6 (DNA topoisomerase 2 OS=Schistocephalus solidus OX=70667 GN=TR96187 PE=3 SV=1) HSP 1 Score: 1444.1 bits (3737), Expect = 0.000e+0 Identity = 715/1156 (61.85%), Postives = 889/1156 (76.90%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQ---------------EDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILE 3557
Y KKTQLEHIL+RPD+Y+GS++ +W++D EK M++K+ITY PGLYKIYDEILVNA+DNK+RD M+CI+IDI N+IRIWNNG GIPVV HR + VP++IFG LLTS+N++D +K+VTGGRNG+GAKLCNIFS +F++ETS+KE+ K FKQTW NM S+P I++ EDFTC++FYPDL RF M+ L+ +T+ALF RRAYDIAA T G+ V LNG ++P+KNFKDYV+LY K+ + E + V+YE RWEVA+ S N F QVSFVNSIATTKGG H+++I+DQ A KL+++ KKK S K V +K Q++ +MWVFV CLIENPSFDSQTKE MTL +K FGSTC L++KFI+QV++ GIVE + W FK Q +DK+C K+KH KLK IPKLDDAN AGTKNS CTLILTEGDSAK+LAV+GL+V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI N+IKI+GLQY KYE PES+ TLRYGK+MIMTDQDQDGSHIKGLLI+FIH WP LL HNF+E+FITPIVK FK Q L+FYSIPE EWQK T + WR+KYYKGLGTSTSKEAK+YFSDM RH I+FRYSG ED SI LAF+K K+D+RK WLT WM + KRR ELG+ E YLYG GT IT+ +F+++ELILFSN DNERSIP L+DG KPGQRKVL+ CLKRNLVKE+KVAQL+GSVAE+S+YHHGEASLM TII LAQDFVGSNN+NLLMP+GQFGTRL+GGKD+ASPRYI T LS T+ IF D+A+L L +DNQ IEP WY P+IPMVLING DGIGTGW +KIPN++ +VI NL+RMI+G P+ M P +R+F G I E+ +N+YVV GEI LD T+EITELP+RVWT SYKE V+E+ +G++K PA I DYKEY+TD TVRF+V M P K EAEA+G HKFFKL T +T ++M+LFD G +++Y + EIL+ FF++RLE Y KRK+YL G+LSA+A +L+NQARF+LEKI GK+ EN LI L Y+ DPV AW++ ++KL AI+ ++ + S D+ Y+ M + +LT E+KD LL +RD+K EL + +KS +++W DDL+ LE
Sbjct: 30 YQKKTQLEHILIRPDTYIGSVERATTLMWLYDKEKQAMIQKEITYTPGLYKIYDEILVNAADNKVRDPSMNCIKIDIKAEDNLIRIWNNGAGIPVVHHRVEKMFVPSLIFGHLLTSSNYDDTEKKVTGGRNGFGAKLCNIFSRKFVVETSSKEHKKCFKQTWIDNMTKTSEPRITASAGEDFTCISFYPDLARFGMTELEADTVALFERRAYDIAASTRGVKVILNGVRVPIKNFKDYVDLYLKDKGEDGEA-VPVVYECVNPRWEVAVAAS-NLGFQQVSFVNSIATTKGGKHIDYISDQVANKLVDVVKKK--SGKSGVTVKSFQIRSHMWVFVNCLIENPSFDSQTKEFMTLQSKSFGSTCVLSEKFINQVSRSGIVENVLSWVRFKAQEKMDKQCHKSKHVKLKGIPKLDDANDAGTKNSAACTLILTEGDSAKTLAVAGLSVVGRDRYGVFPLRGKLLNVREASSKQIMENAEINNLIKILGLQYKNKYETPESVATLRYGKIMIMTDQDQDGSHIKGLLISFIHHNWPKLLHHNFLEEFITPIVKVFKGKQELAFYSIPEFEEWQKTTSNWHTWRVKYYKGLGTSTSKEAKDYFSDMTRHRIRFRYSGPEDDASITLAFNKNKSDDRKSWLTTWMNERKRRVELGLPEDYLYGSGTHAITYSDFVHKELILFSNMDNERSIPSLVDGLKPGQRKVLYTCLKRNLVKEIKVAQLSGSVAEMSSYHHGEASLMGTIIGLAQDFVGSNNLNLLMPHGQFGTRLSGGKDAASPRYIFTSLSPFTRKIFSERDDAILHYLFEDNQRIEPYWYIPVIPMVLINGADGIGTGWGTKIPNYDVMEVISNLRRMIDGADPLPMLPSFRDFRGVIQELGDNRYVVFGEIAILDEQTVEITELPVRVWTQSYKEGVLEVMLNGSEKVPASILDYKEYHTDATVRFVVRMTPEKLREAEAIGLHKFFKLSTAMTTNSMVLFDHMGCVKRYASVSEILKEFFELRLEWYNKRKSYLEGMLSAEARRLENQARFVLEKIQGKISIENRAKRDLIRLLKEHNYEPDPVHAWKECIDKLAAIEDARAASRAAGEGEEEDSEEQGGDASTSSAAADYNYILGMPLWSLTKERKDDLLAQRDKKQAELTELLSKSARRLWRDDLDELE 1181
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: G4VPA9 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 GN=Smp_065840 PE=3 SV=1) HSP 1 Score: 1443.71 bits (3736), Expect = 0.000e+0 Identity = 759/1338 (56.73%), Postives = 974/1338 (72.80%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEE------NQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---------DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKG---DKPTKTNKTNILIEKNAKVSP-------GNKRKRDSGEIIELSDNDDGDS 4070
Y KKTQLEHILLRPDSY+GS + + +W+ D + MV + IT++PGLYKI+DEILVNA+DNK+RD MSCI+IDIN +NM+RIWN+GKGIPVV H+ N +VPT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +FI+ETS+KEY KSF+Q W NM SDP I + + EDFTC+TFYPDL RF M+ L+ +T++LF+RRAYD+AA NGI V+LNG+++P+KNF DYV L+ K D + + +KV+YE RW+VA+ S N F QVSFVNSIATTKGGTH+N+++DQ KL++I KKK S K V++K Q+K +MW+FV CLIENP+FDSQTKENMTL K FGSTC L++KFI Q +K GIVE + W FK +DK+C K+KH+KLK IPKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H WPNLLKHN VE+FITPIVK FK Q SFYS+PE EWQK TP + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED SI LAF K+K +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L L DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N ++I+NL RM++G P +M P +R FTGTI ++ N+YV GE+ LD TLEITELPIRVWT +YKESV+E +G++K PA ITDYKEY+TD TVRF+++M+P K EAE+ G HKFFKLQT ++ +M+ FD G ++ Y N I I+ F+++RL Y KRK YL G+LSA+A KL+NQARF+LEKI ++ EN LI L A YDSDPVKAW++S++K A+ + EE +Q + ++ +PD+ Y+ M + +LT E+KD LL +RD K KEL I+++KSP +W +DL+ LE+E V + + ++ + + K+ K+A ET P RRI P ++ + +KK+ +K+ K + + ++N + EA D N NDE LAQRL + ++ K +G + T++ + + SP GN +++ S + S +D GDS
Sbjct: 36 YQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKK--SGKSGVQIKPFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAWKESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVPTVDADIMKKVEGNKKKRNKTENDPNEGNELDN---------SFEANDEENDGNDEPKPLAQRLGVSEKTDIQKTATTRGRGKGRTVPTSRPKLPKPSSPNNSPIKRKNMGGNAKRKRSSRMPWESGSDTGDS 1360
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A5J4NZ25 (DNA topoisomerase 2 OS=Paragonimus westermani OX=34504 GN=DEA37_0000844 PE=3 SV=1) HSP 1 Score: 1434.85 bits (3713), Expect = 0.000e+0 Identity = 714/1209 (59.06%), Postives = 914/1209 (75.60%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDK----GEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEK---------EINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEE 3722
Y KKTQLEHILLRPDSY+GS +++W+ D EK CMV + IT++PGLYKI+DEILVNA+DNK+RD MSCI++DI+ +NM++IWNNGKGIPVV H+ N++VPT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +FI+ETS+KEY KSF+Q W +NM SDP +S + EDFTCVTFYPDL+RF M+ L+ +T++LF RRAYD+AA TN + VYLNG+++P+K+F DYVELY K D E K++YE RW+VA+ S N F QVSFVNSIATTKGG HV+++ +Q KL+EI KKK S K V++K Q++ +MW+FV CLIENP+FDSQTKENMTL +K FGSTC+L++KFI+Q AKCGIVE + W FK +DK+C K KH KLK IPKLDDAN AGT++S CTLILTEGDSAKSLAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE P+SL+ LRYGK+MIMTDQDQDGSHIKGL+INFIH WP+LLKHN +E+FITPIVK FK + +SFYS+PE WQK TP + WR+KYYKGLGTS+ KEAKEYFSDM RH I+FRY+G ED SI LAF ++K+ +RK WLTNW + KRRRELG+ E YLYGK T+ I++ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNLVKE+KVAQLAGSV+ELSAYHHGE SL+ TI+ LAQ+FVGSNN+NLL+P GQFGTRL+GGKD+AS RYI T LS LT+LIFH D+A+L L DDNQ IEPEWYAPI+PMVL+NG +GIGTG++++IPN++ ++I NL R+++G P+ M P +R F GTI E+ N+YVV+G I LD T+EITELP+R WT +YKESV+E+ +G DK ITDYKEY+TD TVRF+++M P K +AE VG HKFFKL T LT ++M++FD G ++ Y + +L FF +RL+ Y KRK+YL G+LSA+A KL+NQARF+LEKI ++ EN LI L AA YDSDPV+AW++ ++KL +++ G + E +E + PD+ Y+ M + +L+ E+KD LL +RD K EL +R KS +W +DL+ILE+ ++ D +E + K++K A ET P RR+ P ++ E
Sbjct: 41 YQKKTQLEHILLRPDSYIGSTIKTTQSMWVMDEEKQCMVYRDITFSPGLYKIFDEILVNAADNKVRDATMSCIKVDIDPEQNMVKIWNNGKGIPVVHHKVENIYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWIENMTKTSDPKVSVDKGEDFTCVTFYPDLQRFGMNELEADTVSLFVRRAYDLAATTNNVKVYLNGKRLPIKSFMDYVELYVKANKD-EESAPKIVYESNNARWQVAVAAS-NSGFQQVSFVNSIATTKGGKHVDYVAEQLNNKLIEIVKKK--SGKSGVQIKPFQIRNHMWLFVNCLIENPTFDSQTKENMTLQSKSFGSTCSLSEKFITQAAKCGIVESVLSWVRFKAMEKMDKQCHKAKHVKLKGIPKLDDANNAGTRSSSNCTLILTEGDSAKSLAVAGLGVVGRDNYGVFPLRGKLLNVREASSKQIMENAEINALIKILGLQYKLKYETPDSLRDLRYGKVMIMTDQDQDGSHIKGLIINFIHCNWPHLLKHNIIEEFITPIVKVFKGKEEISFYSLPEFEGWQKSTPNWHTWRVKYYKGLGTSSGKEAKEYFSDMVRHRIRFRYTGHEDDDSIQLAFDRSKSSDRKNWLTNWTAERKRRRELGLPEPYLYGKDTRAISYHDFVHRELVLFSNLDNERSIPSMVDGLKPGQRKVLFTCLKRNLVKEIKVAQLAGSVSELSAYHHGEQSLLGTIVGLAQNFVGSNNLNLLLPIGQFGTRLSGGKDAASARYIFTALSPLTRLIFHPADDALLNYLRDDNQRIEPEWYAPIVPMVLVNGAEGIGTGYATRIPNYDVLEIIANLNRLLDGEDPLPMLPSFRGFLGTIQELEANRYVVHGNIAVLDDETVEITELPVRTWTQTYKESVLEVMLNGTDKIQPCITDYKEYHTDTTVRFVIKMTPEKLRDAENVGLHKFFKLSTQLTTNSMVVFDHLGCLKHYQSVSSLLRDFFDLRLQWYEKRKSYLEGMLSAEARKLENQARFVLEKIQSQISIENKPRKELIRMLQAAHYDSDPVRAWKECLDKLATLEEELESGSADRSETVE-TGGPDYNYILGMPLWSLSKERKDDLLAQRDTKQNELRSLRAKSASMLWREDLKILEEAYKKFESELAVDEKVASDAAEKKVLKAATTGTGRTAAQKSLKRAQETKPDPNGRRVLPTVDPE 1244
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0 Identity = 704/1203 (58.52%), Postives = 887/1203 (73.73%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIE--------DSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ--DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD E M ++ITY PGLYKI+DEILVNA+DNK RD+ MS I+I I+ N + IWNNGKGIPVV H+D ++VP +IFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM SDP I +DFTCVTF PDL +F M LD + +AL +RRAYD+A G+ V+LNG+++PV F+ YV+LY K+ LD LKV+ E +RWEV +T S F Q+SFVNSIATTKGG H++++ DQ KL+E+ KKK K+ +V ++WVFV LIENP+FDSQTKENMTL K FGS CAL++KFI CGIVE I W FK Q L+KKCS KH+K+K IPKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+E+FITPIVKA KN Q + FYSIPE EW+K T W +KYYKGLGTSTSKEAKEYF+DMERH I F+Y G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F D +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM++ P+ M P Y+NF G I E+ +N+YVV+GEI+ LD T+EITELPIR WT +YKESV+E G +KTPA I DYKEY+TD TV+F+V M K A+AEA G HK FKLQ+ LT ++M+LFD G +++Y + +IL+ FF++RL Y RK++L+G L A+++KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW ++ EK L EEE+++ E S+ P+F Y+ M + LT EK D LLK+RD K EL+ ++ K + +W +DL + +E++ V Q +++ G+ V +VK K ET PS F RR+EP+I
Sbjct: 54 YQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREITYVPGLYKIFDEILVNAADNKQRDKNMSSIKITIDPESNTVSIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWQNNMTKTSDPKIKYFDGDDFTCVTFQPDLSKFKMEKLDKDIVALLTRRAYDVAGSCKGVKVFLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVVNESVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVSKLIEVVKKKNKAGVTVKPFQVKN---HIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEIPFYSIPEFDEWKKQTENYKTWHIKYYKGLGTSTSKEAKEYFADMERHRIMFKYGGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTATRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCMKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLDHQDPLPMLPSYKNFKGVIHELGQNQYVVSGEISVLDKNTIEITELPIRTWTQAYKESVLEPMLQGTEKTPALINDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQDILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQRGYESDPVAAWNRAQEKAL-----EEEDRDGNESDSSIDSGSSSGPNFNYILNMPLWCLTKEKVDELLKQRDLKKAELNELQRKCSEDLWREDLAVFVEELDRVEEQERESASQGKGVK-LVKGKVGKPKVKKIQLEETLPSPFGRRVEPQI 1245
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1335.47 bits (3455), Expect = 0.000e+0 Identity = 676/1195 (56.57%), Postives = 865/1195 (72.38%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD + NC + IT+ PGLYKI+DEILVNA+DNK RD M+CI+I+I+ N I +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ E SFKQTW NM A + I +D+TC+TF PDL +F M LD +T+AL +RRAYDIA + G+ V LNG ++ V F+ YV+LY K+ +D L V++E RWEV +T S F QVSFVNSIATTKGG H +++ DQ KL+E+ KK+ K +V +MW+FV CLIENPSFDSQTKENMTL K FGSTC L++KFI Q CGIV+ I W FK Q L+KKCS KHTK+K +PKLDDAN AG K+S CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+K NQ SFYSIPE EW++ W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG D ++I LAFS+ K +ERK+WLT++M + ++RRE + E YLYGK T +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L P GQFGTRL GGKDSASPRYI T LS L++L+F D+ +LK DDN +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG P+ M P Y+ F GTI E+ N Y+++GE++ +D TT+EI+ELP++ WT SYKE+V+E+ +G+DK PA ITDY+EY+TD TVRF+V+M + EAEA G HK FKLQ+PLT ++M+LFD G ++KY +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVK W+++ EK + ID GEE+ Q++ E ++ PD+ YL M + LT EKKD L K+RD KL EL+++ K+P +W DL +E+ V + ++ K K +K+ ET P+ RR+ P+I
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVGLNC---RDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVKPFQVKN---HMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVWKQAQEKEVEIDAGEEDEQKE-EQTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKVTQV---KGKGGRTKVVKMKDETLPTPQGRRVIPRI 1202
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1335.47 bits (3455), Expect = 0.000e+0 Identity = 676/1195 (56.57%), Postives = 865/1195 (72.38%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
Y KKTQLEHILLRPD+Y+GS++P + +W+FD + NC + IT+ PGLYKI+DEILVNA+DNK RD M+CI+I+I+ N I +WNNGKGIPVV H+ V VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ E SFKQTW NM A + I +D+TC+TF PDL +F M LD +T+AL +RRAYDIA + G+ V LNG ++ V F+ YV+LY K+ +D L V++E RWEV +T S F QVSFVNSIATTKGG H +++ DQ KL+E+ KK+ K +V +MW+FV CLIENPSFDSQTKENMTL K FGSTC L++KFI Q CGIV+ I W FK Q L+KKCS KHTK+K +PKLDDAN AG K+S CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+K NQ SFYSIPE EW++ W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG D ++I LAFS+ K +ERK+WLT++M + ++RRE + E YLYGK T +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L P GQFGTRL GGKDSASPRYI T LS L++L+F D+ +LK DDN +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG P+ M P Y+ F GTI E+ N Y+++GE++ +D TT+EI+ELP++ WT SYKE+V+E+ +G+DK PA ITDY+EY+TD TVRF+V+M + EAEA G HK FKLQ+PLT ++M+LFD G ++KY +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVK W+++ EK + ID GEE+ Q++ E ++ PD+ YL M + LT EKKD L K+RD KL EL+++ K+P +W DL +E+ V + ++ K K +K+ ET P+ RR+ P+I
Sbjct: 33 YQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVGLNC---RDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVKPFQVKN---HMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVWKQAQEKEVEIDAGEEDEQKE-EQTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKVTQV---KGKGGRTKVVKMKDETLPTPQGRRVIPRI 1202
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1023.85 bits (2646), Expect = 0.000e+0 Identity = 514/876 (58.68%), Postives = 660/876 (75.34%), Query Frame = 3
Query: 1125 NMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYK---GLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKS-VEKLLAIDKGEEENQEDIEDSNK---------PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKI 3713
+MW+FV CL+ENP+FDSQTKENMTL K FGSTC+L++KFI CGIVE I W FK Q+ L++ CS K +KL+ IPKLDDAN AG KNS +CTLILTEGDSAK+LAVSGL V+GRD+YG+FPLRGK+LNVREA KQ+ ENAEI N+IKI+GLQY +KY+ PESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q L FYS+PE EW+ T W++KYYK GLGTSTSKEAKEYF+DM+RH I+FRYSG ED +I LAFS+ K ++RKEWLTNWM D ++RRE G+ E +LYGK T Y+T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI++LAQ+FVGSNNIN+L P GQFGTRL GGKD+ASPRYI T +S + +L+F D+ +LK L DDNQ +EPEWY PIIP+VL+NG DGIGTGW+S+IPNFNP+++I+N++R+++G P+ M PWY+NF G+I E+ N+++ +GEI+ LD T+EI+ELP+R WT +YKESV+E G DKT ITDYKEY+TD TVRF+++++ K EAEA G HK FKLQ PLT ++M+LFD G ++KY + +IL F++VRL+ Y RK +L GIL A+++KL NQARFILEKI G V+ EN LI L A YDSDP++AW+++ V+ + +E+ +D E+S PD+ Y+ M + LT E+KD LL++RD K++EL+ ++ KSP +W DDL + +E++ V ++ + + K G K++ + AE PS RRI P I
Sbjct: 4 HMWIFVNCLVENPTFDSQTKENMTLQHKKFGSTCSLSEKFIKTAMGCGIVESILNWVKFKAQSQLNQNCSAKKISKLRGIPKLDDANDAGGKNSQKCTLILTEGDSAKTLAVSGLGVVGRDHYGIFPLRGKMLNVREASYKQIMENAEINNLIKIVGLQYKKKYDDPESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLQHRFLEEFITPIVKVSKNKQELPFYSMPEFDEWKNETSNYKTWKIKYYKDMPGLGTSTSKEAKEYFADMKRHRIEFRYSGPEDDNAITLAFSRKKIEDRKEWLTNWMVDRRQRREQGLPESFLYGKQTAYLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQSLMMTIVSLAQNFVGSNNINVLQPIGQFGTRLNGGKDAASPRYIFTMISTVARLLFPPVDDNLLKFLWDDNQRVEPEWYCPIIPLVLVNGADGIGTGWASRIPNFNPREIINNIRRLLDGEEPLPMLPWYKNFKGSIDELGPNQFMFSGEISILDSETIEISELPVRTWTQTYKESVLEPMLSGTDKTTPLITDYKEYHTDTTVRFIIKISREKLLEAEAAGLHKVFKLQAPLTCNSMVLFDHLGCLKKYESVADILREFYEVRLKYYSLRKEWLNGILGAESAKLSNQARFILEKIEGTVVIENKPKKELIRMLEARGYDSDPIRAWKEAQVKSNFEEEANSQEDSDDGEESGSATSSTPSSGPDYNYILNMALWCLTKERKDELLRQRDLKIQELEDLKCKSPCDLWKDDLAVFLEELDRVEAKERKDDTADMARGKGGQKKAGSKSLAMKAEVVPSPHGRRIVPVI 879
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007762.1 (pep scaffold:Pmarinus_7.0:GL492656:1017:4077:-1 gene:ENSPMAG00000007010.1 transcript:ENSPMAT00000007762.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 368.622 bits (945), Expect = 3.290e-116 Identity = 176/316 (55.70%), Postives = 230/316 (72.78%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKS 1082
Y KKTQLEHILLRPD+Y+GS++P + +W+FD E M + +TY PGL+KI+DEILVNA+DNK RD M+CI++ I+ N I +WNNGKGIPVV H+ V+VP +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ETS E K FKQ W NM D +I EDFTC+TF PDL +FNM+ LD + +A+ +RRAYDIA G+ V+LNGQ++P+ F+ YV+ Y K+ +D + L V++E +RWEV +T S F QVSFVNSIATTKGG HV+++ DQ KL+E KKK K+
Sbjct: 32 YQKKTQLEHILLRPDTYIGSVEPVTQNMWVFD-EGIGMNCRDVTYVPGLFKIFDEILVNAADNKQRDPNMTCIKVTIDPENNKISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTQFTVETSCSESKKMFKQKWMNNMSKECDSVIKPSSGEDFTCITFQPDLTKFNMTILDKDIMAIMTRRAYDIAGICKGVKVFLNGQKLPLTGFRKYVDFYLKDRVDESGMPLHVVHEVVSNRWEVCLTIS-EKGFQQVSFVNSIATTKGGRHVDYVVDQVTSKLIETVKKKNKA 345
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Match: TOP2 (Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant [Source:SGD;Acc:S000005032]) HSP 1 Score: 1005.36 bits (2598), Expect = 0.000e+0 Identity = 526/1066 (49.34%), Postives = 724/1066 (67.92%), Query Frame = 3
Query: 132 RYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQK-EDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNC-----LDSNEEELK------VIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLK-HNFVEQFITPIVKAF---KNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKL 3281
+Y K +QLEHIL RPD+Y+GS++ +E+ WI+D E +CM+EK +T PGL+KI+DEILVNA+DNK+RD M I ++I+ ++ I + N+GKGIP+ +H N+++P MIFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS EFILET++ G+ + Q W NM + P I+S +K +T VTF PDL RF M LD++ + + RR YDI I VYLNG+ + ++NFK+YVELY K+ LD+ E+ ++YE+ +RWEVA S + +F Q+SFVNSIATT GGTHVN+ITDQ K+ EI KKK K ++ ++K NM++F+ CLIENP+F SQTKE +T KDFGS C + ++I+++ K + ++ A N+ KK T+ +++ + PKL+DAN AGTK +CTL+LTEGDSA SLAV+GLAV+GRDYYG +PLRGK+LNVREA Q+ +NAEI I KI+GLQ+ +KYE K+LRYG LMIMTDQD DGSHIKGL+INF+ S +P LL F+ +FITPI+K ++FY++P+ +W++ W+ KYYKGLGTS ++E +EYFS+++RHL F D I LAFSK KAD+RKEWL R+ E G + K I +FIN+ELILFS DN RSIP ++DGFKPGQRKVL+ C K+NL ELKVAQLA V+E +AYHHGE SL TII LAQ+FVGSNNI LL+PNG FGTR GGKD+A+ RYI T+L+KLT+ IFH D+ + K + +D +++EPEWY PI+PM+L+NG +GIGTGWS+ IP FNP ++I N++ ++N +MHPW+R +TGTI E+ +Y + G I Q+ LEITELP R WT++ KE ++ + GNDK +I D +E + D ++F++ ++P + A+ +GF++ FKL +P++L NM+ FD G I+KYN+ EIL F+ VRLE Y KRK+++ L + K Q +FI I ++ N +I +L
Sbjct: 11 KYQKISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCMIEKNVTIVPGLFKIFDEILVNAADNKVRDPSMKRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFKPDLTRFGMKELDNDILGVMRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLDNGEDGAAKSDIPTILYERINNRWEVAFAVS-DISFQQISFVNSIATTMGGTHVNYITDQIVKKISEILKKKKKKSVKSFQIKN-----NMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIMKTDLATRMFEIAD-ANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYE---DTKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWL--------RQYEPGT----VLDPTLKEIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKEYLL-LGLSGNDKIKPWIKDMEEQHDD-NIKFIITLSPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQEL 1052
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44813 (DNA topoisomerase 2 [Source:UniProtKB/TrEMBL;Acc:A7RUU5]) HSP 1 Score: 546.199 bits (1406), Expect = 7.505e-180 Identity = 279/480 (58.13%), Postives = 361/480 (75.21%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISS-CQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELK------VIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQL 1553
Y KKTQLEHILLRPD+Y+GS++P ++ ++++D +V ++ITY PGL+KI+DEILVNA+DNK RD MS I+I+IN KN I +WN+G+GIP+ MH++ V+VPTMIFGQLLTS+N+NDD+K+VTGGRNGYGAKLCNIFS +F +ET+ E K FKQ WT NMG A++P+I +++TC+TF PDL++F M LD +T+AL +RRAYDIAA G+ V LNG+++ VK FKDYV+L K EE+ + V++E +RWEV +T S + F Q SFVNSIATTKGGTHVN++TDQ KL+ + KKK K ++ ++WVF+ CLIENP+FDSQTKENMTL AKDFGS C L+DKFI V G++E + W FK +A L+KKCS +KH+K++ IPKLDDAN AG KNS CTLILTEGDSAK+LA+SGL+++GRD YGVFPLRGKLLNVREA KQ+
Sbjct: 41 YTKKTQLEHILLRPDTYIGSVEPNKQNMFVYDDILEAIVNREITYVPGLFKIFDEILVNAADNKQRDPAMSTIKIEINPEKNFISVWNDGRGIPIEMHKEHQVYVPTMIFGQLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSKKFSVETACLENKKRFKQVWTNNMGKAAEPVIKEFTSNKEYTCITFQPDLEKFKMETLDRDTVALLTRRAYDIAASVRGVSVILNGKKLKVKTFKDYVQLCLKGATKDKEEDDEEEKKPTVVHEVVNNRWEVCVTVS-DKGFQQASFVNSIATTKGGTHVNYVTDQIVDKLMTVIKKKNKGGLAIKPFQIKS---HLWVFINCLIENPAFDSQTKENMTLRAKDFGSKCELSDKFIKAVQNSGVMENVMNWMRFKEKAQLNKKCSGSKHSKIRGIPKLDDANDAGGKNSRHCTLILTEGDSAKTLAISGLSIVGRDRYGVFPLRGKLLNVREASHKQV 516
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU3]) HSP 1 Score: 349.747 bits (896), Expect = 1.536e-108 Identity = 185/370 (50.00%), Postives = 252/370 (68.11%), Query Frame = 3
Query: 2478 LSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNK----PDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVV 3575
LS ++K++F DE LK L +DNQ IEPEWY PIIP +L+NG +GIGTG+S+K+PN++P+ + +N+++M+NG P +M PWY+NF GTI +V + KY V G I +D TTLEITELPI WT SYKESV+E HG DK P ITDYKEY+T+ VRF++ M K EAE VG HK FKL+ + SNM+LFD G ++KY+ EIL+ FF +R+E YI RK Y+ G+L+A++ KL +QARFILEKI G ++ EN LI LV Y SDPVKAW+ S + L +D +++ + + PDF YL M + +L+ EKKD LLK+RD+K EL+ +R KSP+ +W +DL+I E+ V
Sbjct: 2 LSTVSKMLFPPSDEPNLKFLNEDNQRIEPEWYCPIIPTILVNGAEGIGTGYSTKVPNYDPRAIAENIRKMMNGEEPEEMLPWYKNFIGTITKVDDTKYSVTGNIEVIDDTTLEITELPIGTWTQSYKESVLEPMLHGTDKVPPCITDYKEYHTETRVRFVITMAENKMREAEQVGLHKKFKLEATINTSNMVLFDAMGCLKKYDTTREILKEFFTLRMERYIMRKAYMEGMLTAESGKLNSQARFILEKIEGTIVIENKPKRDLITMLVQRGYPSDPVKAWKDSQDTTLGVDDSDDDTMSTVSSTASGAGGPDFNYLLNMSMWSLSQEKKDELLKQRDQKAAELEDLRKKSPKDLWNEDLDIFLAELEAV 371
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO29547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3B9]) HSP 1 Score: 231.876 bits (590), Expect = 4.711e-70 Identity = 109/170 (64.12%), Postives = 133/170 (78.24%), Query Frame = 3
Query: 1833 LVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL 2342
+ EW+ T W++KYYKGLGTST KEAKEYFSDMERH I F+Y G D ++IV+AFSK K ERK+WLT +E+ K RRE G+ E+YLY K TK +++ +F+N+ELILFSNTDNERSIP L+DG KPGQRKV+F C KRN +E+KVAQLAGS+AELSAYHHGE
Sbjct: 1 MEEWKAGTANHKSWKVKYYKGLGTSTPKEAKEYFSDMERHKINFKYEGPHDDEAIVMAFSKKKIQERKDWLTRGLEERKWRREQGLSELYLYEKDTKRVSYCDFVNKELILFSNTDNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLAGSIAELSAYHHGEVGF 170
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU4]) HSP 1 Score: 159.844 bits (403), Expect = 1.150e-45 Identity = 76/113 (67.26%), Postives = 87/113 (76.99%), Query Frame = 3
Query: 1557 ENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKG 1895
+NAEI NIIKI GLQY++KY+ + LK+LRYG LMIMTDQDQDGSHIKGL+INFIHS WP LLK FVEQFITPIVK K + SFYSIPE EW+ W++KYYKG
Sbjct: 2 DNAEINNIIKITGLQYNKKYKSEDDLKSLRYGHLMIMTDQDQDGSHIKGLVINFIHSNWPGLLKLGFVEQFITPIVKVSKGKEEHSFYSIPEYEEWKAGNANHKSWKVKYYKG 114
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO25698 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVR1]) HSP 1 Score: 79.7221 bits (195), Expect = 5.191e-16 Identity = 57/178 (32.02%), Postives = 91/178 (51.12%), Query Frame = 3
Query: 1401 NSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPES--LKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFY---SIPELVEWQKVTPGANQWRLKYYKGLGTSTSKE 1919
+ QC + L EGDSA A G R++ + PLRGK+LNV +A ++ EN EI N+ +G+ + E P + L +RY K++IM D D DGSHI L++ F +++ +V TP + K Q + + +L+ QK+ G ++ YKGLG +++
Sbjct: 59 DPAQCEIFLVEGDSAGGTAKQGR---DRNFQAILPLRGKILNVEKAMQHKVFENEEIKNMFTALGVSIGTE-EDPRALNLSKVRYHKVVIMCDADVDGSHIATLILTFFFRYMREMIEQGYV-YIATPPLYLVKKGQKREYAWDDNQRDLIA-QKMGGGVT---IQRYKGLGEMNAEQ 227
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 1390.17 bits (3597), Expect = 0.000e+0 Identity = 708/1201 (58.95%), Postives = 886/1201 (73.77%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIE 3716
Y KKT LEHILLRPD+YVGS++P + +W+FD E M +++ITY PGLYKI+DEILVNA+DNK RD+ MS I+I I+ N I IWNNGKGIPVV H+D ++VP +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KEY SFKQTW NM S+P I +DFTCVTF PDL +F M LD +T+AL +RRAYD+A G+ V LNG+++PV F+ YV+LY K LD LKV+ E DRWEV +T S F Q+SFVNSIATTKGG H++++ DQ K++E+ KKK K+ +V ++WVFV LIENP+FDSQTKENMTL K FGS C L++KFI CGIVE I W FK Q L+KKCS KH+K+K IPKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE EW+K T W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F D +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELP+R WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEAVG HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K+ EK A ++GE + + D S+ P+F Y+ M + LT EK + LLK+RD K EL ++ KS + +W +DL + +E++ V Q+ E G + +VK K ET PS F RR++P +
Sbjct: 44 YQKKTPLEHILLRPDTYVGSVEPVSQPMWVFD-EDVGMNQREITYVPGLYKIFDEILVNAADNKQRDKNMSAIKITIDPESNTITIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYRHSFKQTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVKPFQVKN---HIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIK-LVKGKVGKPKAKKMNLEETMPSPFGRRVDPPTQ 1236
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729]) HSP 1 Score: 1370.91 bits (3547), Expect = 0.000e+0 Identity = 714/1307 (54.63%), Postives = 932/1307 (71.31%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW---QKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQ---DTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSG 4031
Y KKTQLEHILLRPDSY+GS++P + +W++D + NC + +T+ PGLYKI+DEILVNA+DNK RD+ MSCI+++I+ N I +WNNGKGIPVV H+ V+VP +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KE K+FKQTW NMG A D I + E+FTC+TF PDL +F MS LD +T+AL +RRAYD+A + G+ V+ NG+++P+ F+ YV++Y K+ +D L VI+E +RWEV +T S F QVSFVNSIATTKGG H++++ DQ +L+E+ KKK K+ +V +MW+FV CL+ENP+FDSQTKENMTL K FGSTC+L+DKF Q CGIVE I W FK Q L+KKCS KHTK+K +PKLDDAN AG KNS+ CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI N+IKI+GLQY + Y PESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+KA LSFYSIPE W++ W++KYYKGLGTSTS+EAKEYFSDMERH I F+YSG ED ++I LAFSK K +ERKEWLTN+M + ++RRE + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPCL+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LK DDNQ +EPEWY PIIP VL+NG +GIGTGW+SKIPN++ ++++ N+ RM++G P+ M P Y+ F GTI +V N+Y+ +GE+ +D TT+EI+ELP++ WT +YKE+V+E +G DK PA ITDYKEY+TD TVRF+V+M K EAEA G HK FKLQ+PLT ++M+LFD G ++KY + +IL+ FF +R++ Y+ RK +L+G+L A++SKL NQARFILEKI G ++ EN LI L YDSDPV+AW QK E + ++ +EE E + S+ D+ YL M + LT EKK+ L K+RD K+ EL+ ++ KSP +W +DL +E+ + + D S P + KG K +K+ ET P+ RR+ P++ + + + E KK K+ + E+ V + G E N E EE LA RL K ++ + L KG + T T + K K +P + + ++G
Sbjct: 30 YQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLSKFKMSTLDKDTVALMTRRAYDVAGSSKGVKVFFNGKRLPITGFRSYVDMYVKDKVDELGGPLTVIHEAVNERWEVCLTMS-EKGFQQVSFVNSIATTKGGRHIDYVADQIVGRLIEVVKKKNKAGVAVKPFQVKN---HMWLFVNCLVENPTFDSQTKENMTLQQKSFGSTCSLSDKFFKQATSCGIVESIMNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSIGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNVIKILGLQYKKNYSDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKNQLSFYSIPEFNAWKETQSNHKSWKIKYYKGLGTSTSQEAKEYFSDMERHRIPFKYSGPEDDEAISLAFSKKKVEERKEWLTNFMVNRRQRREHNLPEDYLYGQSTKSLSYNDFINKELVLFSNSDNERSIPCLVDGFKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSPLARLVFPPVDDNLLKYNYDDNQRVEPEWYIPIIPTVLVNGAEGIGTGWASKIPNYDIREIVSNIHRMLDGEEPLPMLPNYKGFRGTIDQVMSNQYMNSGEVAIIDSTTIEISELPVKTWTQAYKENVLEPMLNGTDKVPALITDYKEYHTDATVRFVVKMTEEKLMEAEAAGLHKVFKLQSPLTCNSMVLFDYAGSLKKYESVQDILKDFFDLRMKYYVLRKEWLVGMLGAESSKLSNQARFILEKIQGTLVIENKPKKELIRMLQEMGYDSDPVRAWKEAQKKDEDDMEAEEEQEEGAEREDQSSGADYNYLLSMPMWFLTKEKKEDLCKQRDAKMTELNTLKGKSPADLWKEDLAAFSEELERLEQKEKDDASAPAK--------RPAKGKKPMKVKQETLPTPQGRRVIPRVTSTMKAEAHKKAELKKGEGRRGKKVKS-EDVVVKMEFGEECE----NLEQIEETGLAARLGKKTKTQTKRALSSKGKQTTLQFST---VSKKPKKNPWSDEESEAG 1313
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 1176 bits (3041), Expect = 0.000e+0 Identity = 606/1042 (58.16%), Postives = 757/1042 (72.65%), Query Frame = 3
Query: 612 QTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIE 3716
QTW NM S+P I +DFTCVTF PDL +F M LD +T+AL +RRAYD+A G+ V LNG+++PV F+ YV+LY K LD LKV+ E DRWEV +T S F Q+SFVNSIATTKGG H++++ DQ K++E+ KKK K+ +V ++WVFV LIENP+FDSQTKENMTL K FGS C L++KFI CGIVE I W FK Q L+KKCS KH+K+K IPKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE EW+K T W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F D +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELP+R WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEAVG HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K+ EK A ++GE + + D S+ P+F Y+ M + LT EK + LLK+RD K EL ++ KS + +W +DL + +E++ V Q+ E G + +VK K ET PS F RR++P +
Sbjct: 123 QTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVKPFQVKN---HIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIK-LVKGKVGKPKAKKMNLEETMPSPFGRRVDPPTQ 1157
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 351.288 bits (900), Expect = 7.900e-109 Identity = 186/372 (50.00%), Postives = 261/372 (70.16%), Query Frame = 3
Query: 2481 SKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQE-----DIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYN 3581
S LTKL+F D +LK L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELP+R WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEAVG HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K+ EK A ++GE + + D S+ P+F Y+ M + LT EK + LLK+RD K EL ++ KS + +W +DL + +E++V ++
Sbjct: 5 SPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEK--AQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDVSFS 374
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729]) HSP 1 Score: 253.447 bits (646), Expect = 3.003e-76 Identity = 118/194 (60.82%), Postives = 153/194 (78.87%), Query Frame = 3
Query: 135 YLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEK--NCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLK 710
Y KKTQLEHILLRPDSY+GS++P + +W++D + NC + +T+ PGLYKI+DEILVNA+DNK RD+ MSCI+++I+ N I +WNNGKGIPVV H+ V+VP +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KE K+FKQTW NMG A D I + E+FTC+TF PDLK
Sbjct: 24 YQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVGLNC---RDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLK 214
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1) HSP 1 Score: 2337.38 bits (6056), Expect = 0.000e+0 Identity = 1288/1435 (89.76%), Postives = 1341/1435 (93.45%), Query Frame = 3
Query: 129 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4430
K Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+ NM+RIWNNG+GIPV +H++ V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM DP IS KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAG K+ H AVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K + KL +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEI ENTNHSDDSSEGAVRKERSKRKAAIKTY
Sbjct: 20 KLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGA-----------------KIALHLVTYAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEIVENTNHSDDSSEGAVRKERSKRKAAIKTY 1437
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1) HSP 1 Score: 2321.2 bits (6014), Expect = 0.000e+0 Identity = 1281/1435 (89.27%), Postives = 1334/1435 (92.96%), Query Frame = 3
Query: 129 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTNHSDDSSEGAVRKERSKRKAAIKTY 4430
K Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+ NM+RIWNNG+GIPV +H++ V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM DP IS KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL+ GKDSASPRYICTQLSKLTKLIFHHDDEAVLK LLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAW K + KL +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEI ENTNHSDDSSEGAVRKERSKRKAAIKTY
Sbjct: 20 KLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLI------------------------------WGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDNNSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPTKTNKTNILIEKNAKVSPGNKRKRDSGEIIELSDNDDGDSNQAQNKVKTNKSEMSSESLFKNIKFPQKQSKLLVENKDKPVAKKAKIEKPKTMKPKTDKPKPQKKDAAPKDKKEAAPKMTKKPAAPKKKEIVENTNHSDDSSEGAVRKERSKRKAAIKTY 1424
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2295.39 bits (5947), Expect = 0.000e+0 Identity = 1205/1302 (92.55%), Postives = 1223/1302 (93.93%), Query Frame = 3
Query: 129 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKP--TKTNKTNILIEKNAKVSPGNKRKRD 4025
KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEK+ITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMH+DVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL +WYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+ ETEKKKK E KKQ ++I V + DN +S + EE LAQRL+ + +EAFK L+ K KP TK +K SP KRKR+
Sbjct: 17 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL----------------------------KWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRN 1287
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2278.83 bits (5904), Expect = 0.000e+0 Identity = 1202/1303 (92.25%), Postives = 1225/1303 (94.01%), Query Frame = 3
Query: 129 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVL-INGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKP--TKTNKTNILIEKNAKVSPGNKRKRD 4025
KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEK+ITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMH+DVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK I M + ++ +G+ + KIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+ ETEKKKK E KKQ ++I V + DN +S + EE LAQRL+ + +EAFK L+ K KP TK +K SP KRKR+
Sbjct: 17 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK----------------ICWMTINLSNQNGMHQLFRCKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRN 1300
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2248.78 bits (5826), Expect = 0.000e+0 Identity = 1193/1302 (91.63%), Postives = 1212/1302 (93.09%), Query Frame = 3
Query: 129 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKQITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHRDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDXXXXXXXXXXXXMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALXXXXXXXXXXXXXXXIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKNLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAVGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAXXXXXXXXXXXXXXXXXXIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVXXXXXXXXXXXXXXXETAPSKFSRRIEPKIEEEYXXXXXXXXXXXXXXXXXXXXXXMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKP--TKTNKTNILIEKNAKVSPGNKRKRD 4025
KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEK+ITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMH+DVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK I + IN + N N DNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEA GFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+ ETEKKKK E KKQ ++I V + DN +S + EE LAQRL+ + +EAFK L+ K KP TK +K SP KRKR+
Sbjct: 17 KRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK-----------------ICWMTIN------------LSNQN-----DNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQKSVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRN 1281 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033538 ID=SMED30033538|Name=DNA topoisomerase 2|organism=Schmidtea mediterranea sexual|type=transcript|length=4567bpback to top protein sequence of SMED30033538-orf-1 >SMED30033538-orf-1 ID=SMED30033538-orf-1|Name=SMED30033538-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1490bp MSSDDSLSLEENESIEKRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDback to top Annotated Terms
The following terms have been associated with this transcript:
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