54S ribosomal protein L4, mitochondrial

Overview
Name54S ribosomal protein L4, mitochondrial
Smed IDSMED30033508
Length (bp)677
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 54S ribosomal protein L4, mitochondrial (SMED30033508) t-SNE clustered cells

Violin plots show distribution of expression levels for 54S ribosomal protein L4, mitochondrial (SMED30033508) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 54S ribosomal protein L4, mitochondrial (SMED30033508) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 54S ribosomal protein L4, mitochondrial (SMED30033508) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033508

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033508h1SMcG0010582 SmedASXL_009963SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30033508h1SMcG0010582 dd_Smed_v4_1861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Human
Match: MRPL47 (mitochondrial ribosomal protein L47 [Source:HGNC Symbol;Acc:HGNC:16652])

HSP 1 Score: 116.316 bits (290), Expect = 2.002e-31
Identity = 61/158 (38.61%), Postives = 91/158 (57.59%), Query Frame = 3 
Query:   30 MSFLCKRFLSKL-----ILNQHKKIFYNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            ++ LC+R  S L     ++            T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER +KV +SM+ L +V++ER++A+  L+ G        ++ D  GR + H  K+
Sbjct:    6 LALLCRRVSSALKSSRSLITPQVPACTGLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQ 163
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Human
Match: MRPL47 (mitochondrial ribosomal protein L47 [Source:HGNC Symbol;Acc:HGNC:16652])

HSP 1 Score: 113.62 bits (283), Expect = 3.365e-30
Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 3 
Query:   93 YNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            Y    T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER +KV +SM+ L +V++ER++A+  L+ G        ++ D  GR + H  K+
Sbjct:   52 YRLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQ 183
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Human
Match: MRPL47 (mitochondrial ribosomal protein L47 [Source:HGNC Symbol;Acc:HGNC:16652])

HSP 1 Score: 47.3654 bits (111), Expect = 2.354e-6
Identity = 24/73 (32.88%), Postives = 43/73 (58.90%), Query Frame = 3 
Query:  270 MIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            M++T+E+E  R     P+ ER +KV +SM+ L +V++ER++A+  L+ G        ++ D  GR + H  K+
Sbjct:    1 MLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQ 73
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Celegans
Match: mrpl-47 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q2L6V1])

HSP 1 Score: 103.99 bits (258), Expect = 2.615e-27
Identity = 54/129 (41.86%), Postives = 76/129 (58.91%), Query Frame = 3 
Query:  126 NLYDFFDEKSNWGIS-IIPSGRP---WRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEP 500
             +  FFD+++N+G + + P  RP   W  +ELR KSN DLH+LWYV LKERNM++TM++ H     N PN ER ++V E+M+ +  V+ ER++AV RLE G    P +       G        EHF P
Sbjct:   26 TMKQFFDDEANFGKAELRPKHRPGRSWTAEELRLKSNSDLHKLWYVCLKERNMLITMKKAHTSRARNMPNPERLDRVHETMDRIESVVHERNDAVFRLETGESAAPRKRTITSFAGFTYQKQATEHFAP 154
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Celegans
Match: mrpl-47 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q2L6V1])

HSP 1 Score: 104.375 bits (259), Expect = 2.932e-27
Identity = 54/129 (41.86%), Postives = 76/129 (58.91%), Query Frame = 3 
Query:  126 NLYDFFDEKSNWGIS-IIPSGRP---WRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEP 500
             +  FFD+++N+G + + P  RP   W  +ELR KSN DLH+LWYV LKERNM++TM++ H     N PN ER ++V E+M+ +  V+ ER++AV RLE G    P +       G        EHF P
Sbjct:   44 TMKQFFDDEANFGKAELRPKHRPGRSWTAEELRLKSNSDLHKLWYVCLKERNMLITMKKAHTSRARNMPNPERLDRVHETMDRIESVVHERNDAVFRLETGESAAPRKRTITSFAGFTYQKQATEHFAP 172
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Fly
Match: mRpL47 (gene:FBgn0014023 transcript:FBtr0082121)

HSP 1 Score: 109.383 bits (272), Expect = 1.018e-28
Identity = 62/132 (46.97%), Postives = 84/132 (63.64%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHV-DGPDEVYKIDELGREVHTVKEEHFEP 500
            TSA   +L +FFD+K NW  + +  GR WR +ELR KSN++LHQLW+VLLKERNM++TME E N     +P+ ER +KV+ SMENL  V+ ER+ A + LE G   + P +  K +  G +V     EH  P
Sbjct:   52 TSAVRRDLMEFFDDKKNWSENEVKVGRAWRTEELRIKSNKELHQLWFVLLKERNMLLTMEHECNDKMEVFPSPERIDKVKISMENLETVVRERNKAYHLLETGETGERPQKAVK-NAFGLQVSYKACEHALP 182
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Zebrafish
Match: mrpl47 (mitochondrial ribosomal protein L47 [Source:ZFIN;Acc:ZDB-GENE-030219-177])

HSP 1 Score: 102.449 bits (254), Expect = 4.235e-26
Identity = 50/107 (46.73%), Postives = 68/107 (63.55%), Query Frame = 3 
Query:   99 SYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGH 419
            S  T+     L +FFD   NWG   + SG PW   +LR KSNEDLH+LWYVLLKE++M++T+E+E  R     P+ ER +KVE SM  L  V+ ER++A+  L+ G 
Sbjct:   58 SLHTTCVRKGLDEFFDLPENWGEETVKSGAPWTAKQLRVKSNEDLHKLWYVLLKEKHMLLTVEQEAKRQCVQMPSPERIKKVERSMIRLDTVVREREDALRLLQTGQ 164
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Zebrafish
Match: mrpl47 (mitochondrial ribosomal protein L47 [Source:ZFIN;Acc:ZDB-GENE-030219-177])

HSP 1 Score: 102.449 bits (254), Expect = 4.235e-26
Identity = 50/107 (46.73%), Postives = 68/107 (63.55%), Query Frame = 3 
Query:   99 SYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGH 419
            S  T+     L +FFD   NWG   + SG PW   +LR KSNEDLH+LWYVLLKE++M++T+E+E  R     P+ ER +KVE SM  L  V+ ER++A+  L+ G 
Sbjct:   58 SLHTTCVRKGLDEFFDLPENWGEETVKSGAPWTAKQLRVKSNEDLHKLWYVLLKEKHMLLTVEQEAKRQCVQMPSPERIKKVERSMIRLDTVVREREDALRLLQTGQ 164
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Xenopus
Match: chn2 (chimerin 2 [Source:Xenbase;Acc:XB-GENE-852930])

HSP 1 Score: 96.6709 bits (239), Expect = 9.290e-24
Identity = 46/108 (42.59%), Postives = 70/108 (64.81%), Query Frame = 3 
Query:   96 NSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGH 419
             S+ +SA    L +FFD+  NWG   + SG  W   +LR K++EDLH+LWYVLLKE+NM++T+E+E  R     P+ ER  KV ++M+ +  VI ER++++  L+ G 
Sbjct:   58 TSFHSSAVCNGLDEFFDDPKNWGEKSVKSGDAWTAKQLREKNSEDLHKLWYVLLKEKNMLLTLEQESKRQRLPMPSPERLSKVGKAMQRIDTVITEREDSLRLLQTGQ 165
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Mouse
Match: Mrpl47 (mitochondrial ribosomal protein L47 [Source:MGI Symbol;Acc:MGI:1921850])

HSP 1 Score: 114.005 bits (284), Expect = 1.835e-30
Identity = 58/127 (45.67%), Postives = 82/127 (64.57%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER EKV +SM+N+ +V++ER++A+  L+ G        ++ D  GR V H  K+
Sbjct:   59 TTLSRKGLEEFFDDPKNWGEEKVKSGASWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLEKVVDSMDNVDKVVQEREDALRLLQTGQEKPRPGAWRRDIFGRIVWHKFKQ 185
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Match: sp|Q08DT6|RM47_BOVIN (39S ribosomal protein L47, mitochondrial OS=Bos taurus OX=9913 GN=MRPL47 PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 8.947e-30
Identity = 64/158 (40.51%), Postives = 92/158 (58.23%), Query Frame = 3 
Query:   24 ILMSFLCKRFLSKLILNQHKKIFYNSYK---TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
             L S  C+  LS L  N      ++  +   T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER EKV +SM+ L +V++ER++A+  L+ G        ++ D  GR + H  K+
Sbjct:   28 TLASTGCRFSLSLLTKNTPNVTSFHQCRLLQTTLSRRGLEEFFDDPKNWGEEKVKSGASWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLEKVVDSMDALDKVVQEREDALRLLQTGQEKPRPGAWRRDIFGRIIWHKFKQ 185
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Match: sp|Q8K2Y7|RM47_MOUSE (39S ribosomal protein L47, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl47 PE=1 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 1.283e-29
Identity = 58/127 (45.67%), Postives = 82/127 (64.57%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER EKV +SM+N+ +V++ER++A+  L+ G        ++ D  GR V H  K+
Sbjct:   59 TTLSRKGLEEFFDDPKNWGEEKVKSGASWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLEKVVDSMDNVDKVVQEREDALRLLQTGQEKPRPGAWRRDIFGRIVWHKFKQ 185
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Match: sp|Q9HD33|RM47_HUMAN (39S ribosomal protein L47, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL47 PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.616e-29
Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 3 
Query:   93 YNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREV-HTVKE 485
            Y    T+ S   L +FFD+  NWG   + SG  W   +LRNKSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER +KV +SM+ L +V++ER++A+  L+ G        ++ D  GR + H  K+
Sbjct:   52 YRLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQ 183
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Match: sp|Q0U6J9|RM04_PHANO (54S ribosomal protein L4, mitochondrial OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=MRPL4 PE=3 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 8.221e-11
Identity = 37/101 (36.63%), Postives = 56/101 (55.45%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPS--GRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYP---NSERFEKVEESMENLIEVIEERDNA 395
            T   +  L+DFF +K      +  S  GR W + ELRN+  + LHQLW+V +KERN + T + E  RL   Y    N +R   V+E+M+ +++ + ER  A
Sbjct:   87 TGTPDHGLWDFFKDKKLIQTPVDESRHGRAWTVGELRNQDWDALHQLWWVCVKERNRLATEKIERARLDAGYGDQENKDRDTVVQETMKAILDTLSERHQA 187
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Match: sp|Q2H080|RM04_CHAGB (54S ribosomal protein L4, mitochondrial OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=MRPL4 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.083e-10
Identity = 35/93 (37.63%), Postives = 53/93 (56.99%), Query Frame = 3 
Query:  126 NLYDFFDEKS---NWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEK---VEESMENLIEVIEER 386
             L++FF  ++   N    I   GR W  +ELR+KS +DLH+LW+V  KERN I T   E N+ G  +  +E  E+   V ++M ++  V+ ER
Sbjct:   98 GLWEFFQNRTMVVNSPPEIAKHGRSWTAEELRHKSWDDLHRLWWVCAKERNRIATANWERNKSGLGFGEAEMRERDNAVRQTMRSIKHVLTER 190
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Match: A0A504YW14 (Mitochondrial ribosomal protein L47 OS=Fasciola gigantica OX=46835 GN=FGIG_03496 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 7.620e-48
Identity = 90/158 (56.96%), Postives = 106/158 (67.09%), Query Frame = 3 
Query:   99 SYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEP----RNRMEDTEMFGQSIVDLLRL 560
            S  T      L DFFD+  NWG S + SGRPWR++ELR KSN DLH+LWYVLLKERNM+MTMEEEHNR     PN ERFEKVEESMENL+ V+EER+ A   LE G   GP  V  +D LGR V  +  EH EP    R+   D +M+G+  V+LLRL
Sbjct:   42 SLHTGQRFQGLLDFFDDPKNWGESRVTSGRPWRMEELRLKSNTDLHELWYVLLKERNMLMTMEEEHNRCLERMPNPERFEKVEESMENLLRVVEERNRAEAELERGEWIGPKVVNGVDILGRPVLKLTCEHDEPQRADRSAQADEQMWGEKTVELLRL 199
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Match: G4VDE9 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_102280 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.609e-47
Identity = 88/178 (49.44%), Postives = 114/178 (64.04%), Query Frame = 3 
Query:   39 LCKRFLSKLILNQHKKIFYNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPR----NRMEDTEMFGQSIVDLLRL 560
            + +R L+     + + + Y+ + T+    +L +FFD+K NWG   + SGRPWR +ELR KSN DLH+LWYVLLKERNM+MTMEEEH R     PN ERFEKVEESMENL+ V+EER+ A + LE G   GP  V  +D LGR V T+  EH  P+    +   D  M+ +  V LLRL
Sbjct:    1 MIRRLLTYFRSLKTESLQYHKFSTTPKLLDLSEFFDDKKNWGEPTVYSGRPWRKEELRLKSNVDLHKLWYVLLKERNMLMTMEEEHFRCLERMPNPERFEKVEESMENLLMVVEERNRAEDELEMGEWVGPKVVESVDPLGRAVQTLTSEHLSPKVIPSHAQSDERMWSEKTVKLLRL 178
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Match: A0A4Z2DNH4 (39S ribosomal protein L47 OS=Schistosoma japonicum OX=6182 GN=EWB00_010724 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 2.089e-47
Identity = 93/187 (49.73%), Postives = 120/187 (64.17%), Query Frame = 3 
Query:   18 FYILMSFLCKRFLSKLILNQHKKIFYNSYK--TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPR----NRMEDTEMFGQSIVDLLRL 560
            F ++   L  RFL        K+  + SYK  T++ + +L +FFD+K NWG + + SGRPWR +ELR KSN DLH+LWY+LLKERNM+MTMEEEH R     PN ERFEKVEESMENL+ V+EER+ A + LE G   GP  V  +D+LGR V T+  EH  P+        D  M+ +  V+LLRL
Sbjct:   48 FSMIGRLLIHRFL--------KRNSFQSYKFSTTSRHLDLNEFFDDKKNWGETTVSSGRPWRKEELRLKSNVDLHKLWYLLLKERNMLMTMEEEHFRCLERMPNPERFEKVEESMENLLMVVEERNRADDELEKGEWVGPKVVEAVDQLGRSVQTLTSEHLSPKVIPSYAQSDESMWSEKTVNLLRL 226
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Match: A0A0R3X7E7 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS9457 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 2.854e-47
Identity = 85/160 (53.12%), Postives = 108/160 (67.50%), Query Frame = 3 
Query:   81 KKIFYNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRME-DTEMFGQSIVDLLR 557
            +K    S   SA   +L +FFD+  NWG+  + +GRPWR+DELR K++ DLH LWY+LLKERNM+MTMEEEH R     PN ERFEKVEESMEN+++VIEERD A+  LE G   GP EV  +D+LGR V  + EEH EP+   + + EMF    +  LR
Sbjct:    9 QKSVSRSISLSAKILDLNEFFDDPKNWGVVSVKAGRPWRVDELRRKNSADLHCLWYILLKERNMLMTMEEEHWRRLERMPNPERFEKVEESMENILQVIEERDKAICELERGEWVGPREVEGVDKLGRPVQRLTEEHLEPQTSTDAEDEMFSTWTLRYLR 168
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Match: A0A183QRM8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 3.327e-47
Identity = 88/178 (49.44%), Postives = 114/178 (64.04%), Query Frame = 3 
Query:   39 LCKRFLSKLILNQHKKIFYNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPR----NRMEDTEMFGQSIVDLLRL 560
            + +R L+     + + + Y+ + T+    +L +FFD+K NWG   + SGRPWR +ELR KSN DLH+LWYVLLKERNM+MTMEEEH R     PN ERFEKVEESMENL+ V+EER+ A + LE G   GP  V  +D LGR V T+  EH  P+    +   D  M+ +  V LLRL
Sbjct:    1 MIRRLLTYFRSLKTESLQYHKFSTTPRLLDLSEFFDDKKNWGEPTVYSGRPWRKEELRLKSNVDLHKLWYVLLKERNMLMTMEEEHFRCLERMPNPERFEKVEESMENLLMVVEERNRAEDELEIGEWVGPKVVESVDPLGRAVQTLTSEHLSPKVIPSHAQSDERMWSEKTVKLLRL 178
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Cavefish
Match: mrpl47 (mitochondrial ribosomal protein L47 [Source:NCBI gene;Acc:103038611])

HSP 1 Score: 108.997 bits (271), Expect = 9.050e-29
Identity = 57/131 (43.51%), Postives = 77/131 (58.78%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEP 500
            TS S   L  FFD   NWG + + SG PW   +LR KSNEDLH+LWYVLLKE+NM++T+E+E  R     P+ ER +KVE SM  L  V+ ER++A+  L+ G   G    ++ +  G+       EH  P
Sbjct:   62 TSCSRLGLDQFFDLPENWGETTVKSGAPWTAKQLRAKSNEDLHKLWYVLLKEKNMLLTIEQESKRQRVPMPSPERIKKVERSMGRLDLVVREREDALRLLQTGQEKGRPGEWRRNIFGKTFWYRCREHPVP 192
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Sea Lamprey
Match: mrpl47 (mitochondrial ribosomal protein L47 [Source:ZFIN;Acc:ZDB-GENE-030219-177])

HSP 1 Score: 95.5153 bits (236), Expect = 2.770e-24
Identity = 46/104 (44.23%), Postives = 65/104 (62.50%), Query Frame = 3 
Query:  108 TSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGH 419
            TS     L +FFD+   WG   + SG  W +  LR KS+EDLH+LWYVLLKE NM++T+E+E  R     P+ ER +KV+ SM+ +  V+ ER+ A+  L+ G 
Sbjct:   53 TSGVRAGLEEFFDDPKRWGEETVKSGSSWGIRLLRGKSSEDLHKLWYVLLKEYNMLLTLEQEAKRQSVPMPSPERIKKVKHSMDRVDGVVREREKALRLLQTGQ 156
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Nematostella
Match: EDO49834 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RER0])

HSP 1 Score: 86.2705 bits (212), Expect = 5.323e-21
Identity = 51/136 (37.50%), Postives = 73/136 (53.68%), Query Frame = 3 
Query:   12 IIFYILMSFLCKRFLSKLILNQHKKIFYNSYKTSASNWNLYDFFD----EKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRL 407
            I  Y+L S   +R +  +             +TS+    L +FF     ++       + +GR WR  ELR KSNEDLH+LWYVLLKERNM+ T+  E  R G   P+ ER+ KV++SM  +  V+ ER+ A+  L
Sbjct:   10 ITKYLLNSASLRRLVPSV---------SRCVQTSSCVRGLEEFFPPGVLDRGELAPEKVKTGRRWRAGELRIKSNEDLHKLWYVLLKERNMLDTLMHEAKRQGVPMPSPERYHKVKKSMAMVKLVLGERERAIQEL 136
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Medaka
Match: mrpl47 (mitochondrial ribosomal protein L47 [Source:NCBI gene;Acc:101157284])

HSP 1 Score: 112.079 bits (279), Expect = 8.275e-30
Identity = 57/133 (42.86%), Postives = 77/133 (57.89%), Query Frame = 3 
Query:   63 LILNQHKKIFYNSYKTSASNWNLYDFFDEKSNWGISIIPSGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELG 461
            + L QH+ +      T+ S   L +FFD   NWG S + SG PW   +LR KSNEDLH+LWYVLLKERNM++T+E+E  R     P+ ER  KVE SM  L   + ER+ A+  L+ G   G    ++ +  G
Sbjct:   60 MPLGQHQSVH-----TTISRRGLEEFFDSPENWGESTVKSGAPWTAKQLRAKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRVPMPSPERLRKVERSMTRLDTAVWERETALRLLQTGQEKGRPGAWRRNMFG 187
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Planmine SMEST
Match: SMESG000002589.1 (SMESG000002589.1)

HSP 1 Score: 210.305 bits (534), Expect = 5.270e-70
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 3 
Query:  276 MTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSXXXXXXXXXXXXXXXXXXXXMVRYDEEIIKKLK 662
            MTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSRENEIAARREEEFWKKENEEMVRYDEEIIKKLK
Sbjct:    1 MTMEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSRENEIAARREEEFWKKENEEMVRYDEEIIKKLK 129
[Close]
BLAST of 54S ribosomal protein L4, mitochondrial vs. Planmine SMEST
Match: SMESG000002589.1 (SMESG000002589.1)

HSP 1 Score: 206.068 bits (523), Expect = 2.819e-68
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 3 
Query:  282 MEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSXXXXXXXXXXXXXXXXXXXXMVRYDEEIIKKLK 662
            MEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSRENEIAARREEEFWKKENEEMVRYDEEIIKKLK
Sbjct:    1 MEEEHNRLGFNYPNSERFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTVKEEHFEPRNRMEDTEMFGQSIVDLLRLSRENEIAARREEEFWKKENEEMVRYDEEIIKKLK 127
The following BLAST results are available for this feature:
BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
MRPL472.002e-3138.61mitochondrial ribosomal protein L47 [Source:HGNC S... [more]
MRPL473.365e-3043.18mitochondrial ribosomal protein L47 [Source:HGNC S... [more]
MRPL472.354e-632.88mitochondrial ribosomal protein L47 [Source:HGNC S... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrpl-472.615e-2741.86Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
mrpl-472.932e-2741.86Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mRpL471.018e-2846.97gene:FBgn0014023 transcript:FBtr0082121[more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrpl474.235e-2646.73mitochondrial ribosomal protein L47 [Source:ZFIN;A... [more]
mrpl474.235e-2646.73mitochondrial ribosomal protein L47 [Source:ZFIN;A... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
chn29.290e-2442.59chimerin 2 [Source:Xenbase;Acc:XB-GENE-852930][more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mrpl471.835e-3045.67mitochondrial ribosomal protein L47 [Source:MGI Sy... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q08DT6|RM47_BOVIN8.947e-3040.5139S ribosomal protein L47, mitochondrial OS=Bos ta... [more]
sp|Q8K2Y7|RM47_MOUSE1.283e-2945.6739S ribosomal protein L47, mitochondrial OS=Mus mu... [more]
sp|Q9HD33|RM47_HUMAN1.616e-2943.1839S ribosomal protein L47, mitochondrial OS=Homo s... [more]
sp|Q0U6J9|RM04_PHANO8.221e-1136.6354S ribosomal protein L4, mitochondrial OS=Phaeosp... [more]
sp|Q2H080|RM04_CHAGB6.083e-1037.6354S ribosomal protein L4, mitochondrial OS=Chaetom... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504YW147.620e-4856.96Mitochondrial ribosomal protein L47 OS=Fasciola gi... [more]
G4VDE91.609e-4749.44Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4Z2DNH42.089e-4749.7339S ribosomal protein L47 OS=Schistosoma japonicum... [more]
A0A0R3X7E72.854e-4753.13Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
A0A183QRM83.327e-4749.44Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl479.050e-2943.51mitochondrial ribosomal protein L47 [Source:NCBI g... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl472.770e-2444.23mitochondrial ribosomal protein L47 [Source:ZFIN;A... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO498345.323e-2137.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl478.275e-3042.86mitochondrial ribosomal protein L47 [Source:NCBI g... [more]
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BLAST of 54S ribosomal protein L4, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000002589.15.270e-70100.00SMESG000002589.1[more]
SMESG000002589.12.819e-68100.00SMESG000002589.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033508 ID=SMED30033508|Name=54S ribosomal protein L4, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=677bp
CTTTTGTTTAAATAATATTTTATATTTTAATGTCTTTTTTATGTAAAAGA
TTTTTGTCAAAGTTAATTTTAAATCAACATAAAAAGATTTTTTATAATTC
ATATAAAACATCAGCTTCTAATTGGAATTTATACGATTTTTTTGATGAAA
AAAGTAATTGGGGTATATCAATCATACCCTCAGGTAGACCGTGGCGTTTG
GATGAGCTAAGAAATAAAAGTAATGAAGATCTTCATCAGTTGTGGTATGT
TCTTTTAAAAGAGAGGAATATGATAATGACAATGGAAGAAGAGCATAACC
GACTTGGATTTAACTATCCTAATTCTGAAAGATTTGAAAAGGTAGAAGAA
AGTATGGAAAATTTGATAGAAGTGATTGAAGAGAGAGATAATGCTGTCAA
CCGCCTCGAATATGGACACGTTGATGGACCTGATGAAGTGTATAAAATAG
ATGAGCTCGGCCGGGAAGTTCACACTGTAAAAGAAGAACATTTTGAGCCA
CGAAATCGAATGGAAGATACGGAAATGTTTGGTCAATCTATAGTCGATCT
ATTGCGACTTTCGAGAGAAAATGAAATTGCTGCTCGAAGAGAGGAAGAAT
TTTGGAAGAAGGAAAACGAAGAAATGGTTAGATATGACGAGGAGATTATC
AAAAAGTTGAAATAAAATTCGCAGATC
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protein sequence of SMED30033508-orf-1

>SMED30033508-orf-1 ID=SMED30033508-orf-1|Name=SMED30033508-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=212bp
MSFLCKRFLSKLILNQHKKIFYNSYKTSASNWNLYDFFDEKSNWGISIIP
SGRPWRLDELRNKSNEDLHQLWYVLLKERNMIMTMEEEHNRLGFNYPNSE
RFEKVEESMENLIEVIEERDNAVNRLEYGHVDGPDEVYKIDELGREVHTV
KEEHFEPRNRMEDTEMFGQSIVDLLRLSRENEIAARREEEFWKKENEEMV
RYDEEIIKKLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR038340MRP-L47_sf
IPR010729Ribosomal_L47_mit
Vocabulary: biological process
TermDefinition
GO:0006412translation
GO:0032543mitochondrial translation
Vocabulary: cellular component
TermDefinition
GO:0005761mitochondrial ribosome
GO:0005739mitochondrion
GO:0005762mitochondrial large ribosomal subunit
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010729Ribosomal protein L47, mitochondrialPFAMPF06984MRP-L47coord: 34..119
e-value: 3.3E-29
score: 100.8
IPR010729Ribosomal protein L47, mitochondrialPANTHERPTHR21183RIBOSOMAL PROTEIN L47, MITOCHONDRIAL-RELATEDcoord: 27..198
IPR038340MRP-L47 superfamily, mitochondrialGENE3DG3DSA:1.20.1280.190coord: 28..128
e-value: 2.6E-23
score: 83.9