Calcium activated potassium channel subunit

Overview
NameCalcium activated potassium channel subunit
Smed IDSMED30033475
Length (bp)884
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium activated potassium channel subunit (SMED30033475) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium activated potassium channel subunit (SMED30033475) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium activated potassium channel subunit (SMED30033475) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium activated potassium channel subunit (SMED30033475) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033475

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30033475h1SMcG0001455 Contig3273newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033475h1SMcG0001455 Contig3273uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033475h1SMcG0012766 Contig3273newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033475h1SMcG0012766 Contig3273uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30033475h1SMcG0001455 SmedASXL_067101SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30033475h1SMcG0001455 dd_Smed_v6_14020_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
enteric muscle cellSMED30033475h1SMcG0001455 dd_Smed_v4_14020_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A1I8G080 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 4.734e-36
Identity = 78/217 (35.94%), Postives = 121/217 (55.76%), Query Frame = 2
Query:  227 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKH---KVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFY----KDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            M ++  D++CN +G++K+ +LA  TL T G+I+  IL+ L+++PY  E ++ +  C Y     I K+    CE+KC+K+ S F C  I+VS  + +      G L++N  TFS+     +++C T PCL    +N      F  +L +   F C Y    K    T VLL+++ S     +CI+WP   +  GL  L  T+YCAGC +W+QDKTVIA
Sbjct:    1 MALKRCDRLCNVHGLAKLIFLACITLITCGVIVGTILVPLIILPYSRESRFADANCTYLTEHLIAKL---PCESKCSKDKSSFPCYSIRVSISQTNQT----GMLYENFITFSERHDEPINQCGTAPCLSQAAENVAMTTRFIRTLRKSRSFPCHYQPASKPGGPTPVLLHRIFSRRQATHCIIWPAVAVAAGLSLLVSTYYCAGCTVWTQDKTVIA 210          
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A1I8G034 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.722e-35
Identity = 78/217 (35.94%), Postives = 121/217 (55.76%), Query Frame = 2
Query:  227 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKH---KVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFY----KDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            M ++  D++CN +G++K+ +LA  TL T G+I+  IL+ L+++PY  E ++ +  C Y     I K+    CE+KC+K+ S F C  I+VS  + +      G L++N  TFS+     +++C T PCL    +N      F  +L +   F C Y    K    T VLL+++ S     +CI+WP   +  GL  L  T+YCAGC +W+QDKTVIA
Sbjct:    1 MALKRCDRLCNVHGLAKLIFLACITLITCGVIVGTILVPLIILPYSRESRFADANCTYLTEHLIAKL---PCESKCSKDKSSFPCYSIRVSISQTNQT----GMLYENFITFSERHDEPINQCGTAPCLSQAAENVAMTTRFIRTLRKSRSFPCHYQPASKPGGPTPVLLHRIFSRRQATHCIIWPAVAVAAGLALLVSTYYCAGCTVWTQDKTVIA 210          
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A1I8GE69 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.304e-31
Identity = 71/213 (33.33%), Postives = 121/213 (56.81%), Query Frame = 2
Query:  227 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDET---TNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            M ++   ++CN  GI+K  +L   +L T G+I+  IL YL+++PY  E ++  T+C Y  G ++ ++I   CE+KC K+ S + C  ++V+   +  +    G LF+N  T+ +     CS+ PCL    +N      + ++L RR  F C+++  ++    +VLL K+ +     +C +WP   +++G I L  T YCAGC +W+QDKTVIA
Sbjct:    1 MALRRCHRLCNVMGIAKYVFLTCVSLITCGVIVGGILAYLIILPYYREARFEQTSCVYL-GEEMKRII--DCESKCAKDRSSYPCYMVRVNISGVPEQ----GVLFENFLTYLEQDKTNCSSAPCLPEAVENVEMTRRYIKTLRRRRNFTCYFRGPDSRQKNSVLLTKIYTKEQAKHCAIWPAFAVSIGAIVLIGTHYCAGCTVWTQDKTVIA 206          
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A1I8HKS0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.450e-31
Identity = 71/213 (33.33%), Postives = 121/213 (56.81%), Query Frame = 2
Query:  227 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDET---TNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            M ++   ++CN  GI+K  +L   +L T G+I+  IL YL+++PY  E ++  T+C Y  G ++ ++I   CE+KC K+ S + C  ++V+   +  +    G LF+N  T+ +     CS+ PCL    +N      + ++L RR  F C+++  ++    +VLL K+ +     +C +WP   +++G I L  T YCAGC +W+QDKTVIA
Sbjct:    1 MALRRCHRLCNVIGIAKYVFLTCVSLITCGVIVGGILAYLIILPYYREARFEQTSCVYL-GEEMKRII--DCESKCAKDRSSYPCYMVRVNISGVPEQ----GVLFENFLTYLEQDKTNCSSAPCLPEAVENVEMTRRYIKTLRRRRNFTCYFRGPDSRQKNSVLLTKIYTKEQAKHCAIWPAFAVSIGAIVLIGTHYCAGCTVWTQDKTVIA 206          
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A4S2LYB8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004041 PE=4 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.632e-31
Identity = 77/194 (39.69%), Postives = 106/194 (54.64%), Query Frame = 2
Query:  275 KVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            K+TYL  AT+ T  LIIECILL LVV+PYL E  +  T C Y  GS+I +  +K CENKC+K+ S F C+ + V+Y K  + Q     LFDN+ T+  +    C+T  C    E NR     F++ + R   F CF  +D     LL+K   P  + N + WP TLL +  + L + W    C+ W  + TVIA
Sbjct:   55 KITYLTCATVITTSLIIECILLRLVVMPYLHESGFSATRCVYI-GSRIIQNAVK-CENKCSKDRSTFQCIRVMVAYAK--NNQNYTANLFDNIATYQHYHTLGCATSSCQRRNEANRASVEEFHQLIRRSARFDCFTHEDHVNEALLHKFYGPATLFNTLFWPLTLLVISSLVLMVVWIYDRCRAWGDENTVIA 244          
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000032303.1 (SMESG000032303.1)

HSP 1 Score: 404.06 bits (1037), Expect = 4.443e-144
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 2
Query:  227 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCTLLTLGLISLFLTWYCAGCKIWSQDKTVIA
Sbjct:    1 MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCTLLTLGLISLFLTWYCAGCKIWSQDKTVIA 210          
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000053268.1 (SMESG000053268.1)

HSP 1 Score: 162.925 bits (411), Expect = 3.636e-49
Identity = 89/208 (42.79%), Postives = 134/208 (64.42%), Query Frame = 2
Query:  254 CNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILK-----KCENKCTKETSFFYCLFIKVSYKKIHS--KQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            CN  GISK+ YL+S T  T+G I+ECILL+L++ PYL+EQ++  TTC Y   S   + IL      +CENKCTK+T  F C+++ V+Y   +S  +QL  GYL+D  RT++++K  +CST PC  LL DN  W ++F+ +LY +  F C+Y   +  +VLLYKL   +++++CI+WP  +  +GL+ +  T   AGC +W  +  VIA
Sbjct:   13 CNETGISKLIYLSSMTFLTSGFILECILLHLMLTPYLAEQEFEQTTCVYRNSS--SRFILLSDLQLRCENKCTKDTGKFPCVWLLVNYVIPNSGQRQLQDGYLYDYYRTYNRYKKQRCSTSPCHLLLSDNIDWVISFDRTLYSKHNFSCYYNTKDHMSVLLYKLYPKSLILHCILWPFGIFIVGLVGITFTALIAGCDVWKNEDVVIA 218          
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000017226.1 (SMESG000017226.1)

HSP 1 Score: 119.398 bits (298), Expect = 3.518e-32
Identity = 65/194 (33.51%), Postives = 109/194 (56.19%), Query Frame = 2
Query:  275 KVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            K+ YLAS ++F +  I+ CIL++LV+IPYL E  +I+  C+  E +++G  +  +CENKC+K+ S F CL I V+Y++  + ++   YL++++ T      + C+T PC     DN      FN  + R+  F CF      +  LLYK    +  ++C +WP  L+     SL + +    C++W  D+TVI+
Sbjct:   40 KIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNE-TRMGDEV--RCENKCSKDRSKFKCLQIIVNYRR--NNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 228          
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000055809.1 (SMESG000055809.1)

HSP 1 Score: 105.145 bits (261), Expect = 6.365e-27
Identity = 69/208 (33.17%), Postives = 109/208 (52.40%), Query Frame = 2
Query:  263 YGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYK------KIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDN----RRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQDKTVIA 856
            + + K  Y++S ++  +G IIECILL+L++ PY  E ++++  C     S    +   KC NKC+KE S F+C  +KV Y+      ++ ++    GYLFD + T    K +KC   PC  L  DN    R +     + L   + F C+     T  VLLYK  S    ++CI+WP TL+ L +  + L ++   CK+W+ DK +I 
Sbjct:   10 HSVLKFLYISSLSVAVSGFIIECILLHLIISPYGHETQFVDGKCTVLNISTRYGI---KCVNKCSKERSKFFCSTVKVKYQSKISLGRLSNQTWYHGYLFDQITTLHNFKDEKCIISPCYKLKLDNYIETRNFLHVHFDHLESNSAFPCYVNRYLTNEVLLYKFYSYETYISCILWPITLIVLSVFIILLCYWIDRCKVWANDKAIIT 214          
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000047387.1 (SMESG000047387.1)

HSP 1 Score: 93.5893 bits (231), Expect = 7.255e-23
Identity = 57/188 (30.32%), Postives = 95/188 (50.53%), Query Frame = 2
Query:  275 KVTYLASATLFTAGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCXXXXXXXXXXXXXWYCAGCKIWSQ 838
            K  Y++ +TL    LI+  IL++LVVIP+  E  + ++TC     +    +   KCENKC+K+ S F CL I   Y  +   Q  + Y++D++ T S ++  +C+  PC    +DN      F   + +   F C+   +    VLLYK  S  +  +C+ WP  LL + L+ L + +    C +W+ 
Sbjct:   14 KFLYISCSTLSLCALIVGAILIHLVVIPFTHENSFYSSTCTLINTNITTSI---KCENKCSKDRSMFPCLQITAKYTDL--DQEFLAYVYDHIATLSHYRKSQCAISPCFSSTKDNGIIVSEFQSRMRKTPTFACYINAEHRDEVLLYKFYSIGMFWHCVAWPSGLLVISLLILAVLYSKDKCNVWND 196          
The following BLAST results are available for this feature:
BLAST of Calcium activated potassium channel subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8G0804.734e-3635.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8G0343.722e-3535.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GE691.304e-3133.33Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HKS01.450e-3133.33Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A4S2LYB81.632e-3139.69Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032303.14.443e-144100.00SMESG000032303.1[more]
SMESG000053268.13.636e-4942.79SMESG000053268.1[more]
SMESG000017226.13.518e-3233.51SMESG000017226.1[more]
SMESG000055809.16.365e-2733.17SMESG000055809.1[more]
SMESG000047387.17.255e-2330.32SMESG000047387.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033475 ID=SMED30033475|Name=Calcium activated potassium channel subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=884bp
CACTGGGCAAGTCAGCCTCAAACTCACACACCAGCAAGCAAGAGATAATG
TTGATACTGTAGAATACTGAGTCCATTTTCACGAGCTAAATTTACTTCGT
TTTCATGAAATTTTAAGAGATTGTGCATCTCATAATAATAATGATCATGA
ACTCCACAATTCTAGTTGTTGCGTAGTGGGACAGTGCAAAATTTCCTACT
GTACAAACGACAAGTTAAAATCTTCAATGACTGTGCAAGCTCTGGATAAG
ATTTGCAACTATTACGGGATCAGCAAAGTGACTTATCTTGCAAGCGCGAC
TCTTTTCACTGCCGGTTTAATTATAGAATGTATTCTCCTATATCTTGTCG
TAATTCCGTATCTCTCTGAACAGAAGTACATTAATACAACTTGCCATTAT
ACTGAAGGGTCGAAAATCGGGAAAATGATTTTGAAAAAATGCGAAAACAA
ATGCACAAAAGAAACGAGTTTTTTCTATTGTTTATTTATCAAAGTTTCCT
ATAAAAAAATCCACTCAAAACAACTGACAATCGGATATTTGTTTGACAAT
CTACGAACGTTTTCCAAGCACAAAGTTGACAAGTGCTCTACAATGCCATG
TTTAGGACTGCTAGAAGACAACCGCAGATGGGCAATGGCATTCAATGAAT
CCTTATACCGCCGAACCTATTTTCAATGCTTTTATAAAGACGATGAAACG
ACAAATGTGTTACTGTATAAACTCCAGTCTCCAACAATTGTCATGAATTG
TATTGTTTGGCCATGCACATTATTGACGTTGGGTCTCATCAGTTTATTTT
TAACGTGGTATTGTGCCGGCTGTAAGATATGGAGCCAAGATAAAACTGTT
ATTGCTTGAATTTTATTTATTGTAAATGGAAAAT
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protein sequence of SMED30033475-orf-1

>SMED30033475-orf-1 ID=SMED30033475-orf-1|Name=SMED30033475-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp
MTVQALDKICNYYGISKVTYLASATLFTAGLIIECILLYLVVIPYLSEQK
YINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQL
TIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQ
CFYKDDETTNVLLYKLQSPTIVMNCIVWPCTLLTLGLISLFLTWYCAGCK
IWSQDKTVIA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000418head
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR003930K_chnl_Ca-activ_BK_bsu
Vocabulary: molecular function
TermDefinition
GO:0015269calcium-activated potassium channel activity
Vocabulary: biological process
TermDefinition
GO:0006813potassium ion transport
GO:0071805potassium ion transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003930Potassium channel, calcium-activated, BK, beta subunitPFAMPF03185CaKBcoord: 29..192
e-value: 4.5E-11
score: 42.8
IPR003930Potassium channel, calcium-activated, BK, beta subunitPANTHERPTHR10258CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETAcoord: 19..207
NoneNo IPR availablePANTHERPTHR10258:SF8coord: 19..207
NoneNo IPR availableTMHMMTMhelixcoord: 21..43
NoneNo IPR availableTMHMMTMhelixcoord: 170..192