Nascent polypeptide-associated complex subunit beta
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033402 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13
Homology
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125]) HSP 1 Score: 157.918 bits (398), Expect = 1.145e-48 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125]) HSP 1 Score: 157.147 bits (396), Expect = 8.227e-48 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 53 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 202
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:HGNC Symbol;Acc:HGNC:30547]) HSP 1 Score: 147.902 bits (372), Expect = 7.100e-45 Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K ++EMLP + QLG ++ +++K+AE+ R+ +++K+ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:HGNC Symbol;Acc:HGNC:30547]) HSP 1 Score: 134.806 bits (338), Expect = 6.241e-40 Identity = 71/126 (56.35%), Postives = 97/126 (76.98%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS 458 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K ++EMLP + QLG ++ +++K+AE+ R+ +++K+ Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKA 129
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125]) HSP 1 Score: 92.0485 bits (227), Expect = 6.310e-24 Identity = 46/97 (47.42%), Postives = 62/97 (63.92%), Query Frame = 3 Query: 234 IEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524 IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + + LVENFDE SK Sbjct: 2 IEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQY----------------LVENFDEASK 82
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Match: icd-1 (Transcription factor BTF3 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18885]) HSP 1 Score: 140.584 bits (353), Expect = 1.967e-42 Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 3 Query: 75 DRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524 +R++KL ++VRIGGKG+ RRKKKV+ K +D+KKLQ+ LK+L V IP IEEVNM KDDGT+IHF NPKVQ S+ NT S++G ++ K ++EMLP + QLGP ++ ++KK+A N+ ED+DVP LV +FD SK Sbjct: 8 ERIKKLQAQQEHVRIGGKGTPRRKKKVIHKTAAADDKKLQSNLKKLSVTNIPGIEEVNMIKDDGTVIHFNNPKVQTSVPANTFSVTGSADNKQITEMLPGILNQLGPESLTHLKKLA----NNVTKLGPDGKGEDEDVPELVGDFDAASK 153
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Match: icd-1 (Transcription factor BTF3 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18885]) HSP 1 Score: 140.584 bits (353), Expect = 1.967e-42 Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 3 Query: 75 DRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524 +R++KL ++VRIGGKG+ RRKKKV+ K +D+KKLQ+ LK+L V IP IEEVNM KDDGT+IHF NPKVQ S+ NT S++G ++ K ++EMLP + QLGP ++ ++KK+A N+ ED+DVP LV +FD SK Sbjct: 8 ERIKKLQAQQEHVRIGGKGTPRRKKKVIHKTAAADDKKLQSNLKKLSVTNIPGIEEVNMIKDDGTVIHFNNPKVQTSVPANTFSVTGSADNKQITEMLPGILNQLGPESLTHLKKLA----NNVTKLGPDGKGEDEDVPELVGDFDAASK 153
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: bic (gene:FBgn0000181 transcript:FBtr0087782) HSP 1 Score: 135.191 bits (339), Expect = 5.293e-40 Identity = 79/148 (53.38%), Postives = 102/148 (68.92%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM----RENIENKSTSQINEDDDVPTLVENFDE 515 EKL KL VRIGGKG+ RRKKK+V +D+KKLQ++LK+L VN IP IEEVN+ K+DGT+IHF NPK QASL NT +I+G E K ++EM+P + QLGP +I +KK+A E+ S++ DDDVP LVENF+E Sbjct: 4 EKLKKLQAQVRIGGKGTPRRKKKIVHSTPATDDKKLQSSLKKLSVNTIPGIEEVNIIKNDGTVIHFNNPKAQASLPTNTFAITGHGENKTITEMVPGILTQLGPQDINQLKKLATEIASKSGAGGAAGSSAADAGDDDVPDLVENFEE 151
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: bic (gene:FBgn0000181 transcript:FBtr0087783) HSP 1 Score: 135.191 bits (339), Expect = 5.293e-40 Identity = 79/148 (53.38%), Postives = 102/148 (68.92%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM----RENIENKSTSQINEDDDVPTLVENFDE 515 EKL KL VRIGGKG+ RRKKK+V +D+KKLQ++LK+L VN IP IEEVN+ K+DGT+IHF NPK QASL NT +I+G E K ++EM+P + QLGP +I +KK+A E+ S++ DDDVP LVENF+E Sbjct: 4 EKLKKLQAQVRIGGKGTPRRKKKIVHSTPATDDKKLQSSLKKLSVNTIPGIEEVNIIKNDGTVIHFNNPKAQASLPTNTFAITGHGENKTITEMVPGILTQLGPQDINQLKKLATEIASKSGAGGAAGSSAADAGDDDVPDLVENFEE 151
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0332341) HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34 Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3 Query: 81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530 +EKL +L VRIGGKG+ RRKKKV+ + +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL NT +++G E + + EMLP + QLG + ++ A M + EDDDVP LV +FDE +K++ Sbjct: 3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0332342) HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34 Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3 Query: 81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530 +EKL +L VRIGGKG+ RRKKKV+ + +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL NT +++G E + + EMLP + QLG + ++ A M + EDDDVP LV +FDE +K++ Sbjct: 3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0078076) HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34 Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3 Query: 81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530 +EKL +L VRIGGKG+ RRKKKV+ + +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL NT +++G E + + EMLP + QLG + ++ A M + EDDDVP LV +FDE +K++ Sbjct: 3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731]) HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46 Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + ++ + K+ E DD+VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731]) HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46 Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + ++ + K+ E DD+VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731]) HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46 Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + ++ + K+ E DD+VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731]) HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46 Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + ++ + K+ E DD+VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:393667]) HSP 1 Score: 150.599 bits (379), Expect = 3.585e-46 Identity = 88/151 (58.28%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++K+AE+ R+ ++N K+ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLVVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: ttc34 (tetratricopeptide repeat domain 34 [Source:Xenbase;Acc:XB-GENE-991680]) HSP 1 Score: 154.066 bits (388), Expect = 1.065e-46 Identity = 85/150 (56.67%), Postives = 113/150 (75.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ S EDD+VP LVENFDE SK Sbjct: 56 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLASGEEEDDEVPELVENFDEASK 205
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:496686]) HSP 1 Score: 149.058 bits (375), Expect = 2.242e-45 Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E+K ++EMLP + QLG ++ +++K+AE+ R+ +++K++ EDDDVP LV NFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: ttc34 (tetratricopeptide repeat domain 34 [Source:Xenbase;Acc:XB-GENE-991680]) HSP 1 Score: 140.584 bits (353), Expect = 2.089e-41 Identity = 82/169 (48.52%), Postives = 116/169 (68.64%), Query Frame = 3 Query: 27 LNLFIIKYMESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524 LN + +V + K R + L V + G+G+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ S EDD+VP LVENFDE SK Sbjct: 32 LNPLCSPGVPAVCLQKVR-GRYRVLGGCVWVSGQGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLASGEEEDDEVPELVENFDEASK 199
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875]) HSP 1 Score: 157.918 bits (398), Expect = 7.863e-49 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875]) HSP 1 Score: 157.918 bits (398), Expect = 7.863e-49 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875]) HSP 1 Score: 156.762 bits (395), Expect = 5.807e-48 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 51 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 200
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3l4 (basic transcription factor 3-like 4 [Source:MGI Symbol;Acc:MGI:1915312]) HSP 1 Score: 147.902 bits (372), Expect = 4.875e-45 Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K ++EMLP + QLG ++ +++K+AE+ R+ +++K+ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3l4 (basic transcription factor 3-like 4 [Source:MGI Symbol;Acc:MGI:1915312]) HSP 1 Score: 147.902 bits (372), Expect = 4.875e-45 Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K ++EMLP + QLG ++ +++K+AE+ R+ +++K+ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|P20290|BTF3_HUMAN (Transcription factor BTF3 OS=Homo sapiens OX=9606 GN=BTF3 PE=1 SV=1) HSP 1 Score: 157.147 bits (396), Expect = 3.951e-47 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 53 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 202
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q64152|BTF3_MOUSE (Transcription factor BTF3 OS=Mus musculus OX=10090 GN=Btf3 PE=1 SV=3) HSP 1 Score: 156.762 bits (395), Expect = 4.062e-47 Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++++AE + +++++ K+ EDDD VP LVENFDE SK Sbjct: 51 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 200
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q6PC91|BT3L4_DANRE (Transcription factor BTF3 homolog 4 OS=Danio rerio OX=7955 GN=btf3l4 PE=2 SV=1) HSP 1 Score: 150.599 bits (379), Expect = 2.706e-45 Identity = 88/151 (58.28%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++K+AE+ R+ ++N K+ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLVVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q5M8V0|BT3L4_XENTR (Transcription factor BTF3 homolog 4 OS=Xenopus tropicalis OX=8364 GN=btf3l4 PE=2 SV=1) HSP 1 Score: 149.058 bits (375), Expect = 1.188e-44 Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E+K ++EMLP + QLG ++ +++K+AE+ R+ +++K++ EDDDVP LV NFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q4KLF5|BT3L4_XENLA (Transcription factor BTF3 homolog 4 OS=Xenopus laevis OX=8355 GN=btf3l4 PE=2 SV=1) HSP 1 Score: 149.058 bits (375), Expect = 1.188e-44 Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E+K ++EMLP + QLG ++ +++K+AE+ R+ +++K++ EDDDVP LV NFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A2R7WAG3 (Transcription factor BTF3 OS=Oncopeltus fasciatus OX=7536 GN=OFAS_OFAS006791 PE=3 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 5.509e-48 Identity = 85/147 (57.82%), Postives = 105/147 (71.43%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ RRKKKV+ +D+KKLQ++LK+L VN IP IEEVNM KDDGT+IHF NPK QASL NT +++G E K ++EMLP + QLGP I +K++A + N K+T I EDD+VP LVENFDE SK Sbjct: 4 EKLKKLQAQVRIGGKGTPRRKKKVLHATAATDDKKLQSSLKKLSVNTIPGIEEVNMIKDDGTVIHFNNPKAQASLAANTFAVTGHGETKQITEMLPGILSQLGPEGINQLKRLATSVAGNTAGKAT--IEEDDEVPDLVENFDEASK 148
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: B5XE39 (Transcription factor BTF3 OS=Salmo salar OX=8030 GN=BT3L4 PE=2 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.224e-47 Identity = 88/150 (58.67%), Postives = 113/150 (75.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKGSARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDG++IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++K+AE+ R+ +++K+ I EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGSARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGSVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKTPKAEDIEEDDDVPDLVENFDEASK 153
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: C3YIK9 (Transcription factor BTF3 OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_131258 PE=3 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.417e-47 Identity = 85/157 (54.14%), Postives = 116/157 (73.89%), Query Frame = 3 Query: 66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRE----NIENKSTSQINEDDDVPTLVENFDEQSK 524 +N+++L ++ K+S+ VRIGGKGSARRK+KVV + +D+KKLQ++LK+L VN IP IEEVNM +DDGT++HF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ N+KK+AE + + + T+ EDDDVP LV NFDE SK Sbjct: 1 MNQEKLNRMQKMSEQVRIGGKGSARRKRKVVHRTATTDDKKLQSSLKKLTVNNIPGIEEVNMIRDDGTVVHFNNPKVQASLAANTFAITGHAENKQLTEMLPGILNQLGADSLSNLKKLAESLPSQDFFSPFSAPTATAGEDDDVPELVANFDEPSK 157
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A1I8GPL3 (Transcription factor BTF3 OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.579e-47 Identity = 90/159 (56.60%), Postives = 115/159 (72.33%), Query Frame = 3 Query: 78 RLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-----RENIENKSTSQIN------EDDDVPTLVENFDEQS 521 +++KL D++RIGGKGSARRKKKV+ KNN +DE+K+ TTLK+L V+ IP IEEVNMFKD G +IHF NPKVQASLQ NT +ISGQSE+KNL+E+LPS+W QLGP N N+KK+A + + + E S + ++DDVP LV NFD S Sbjct: 6 KIDKLKAQVDSIRIGGKGSARRKKKVIHKNNATDERKVTTTLKKLAVSSIPGIEEVNMFKDSGEVIHFNNPKVQASLQSNTFAISGQSEVKNLTELLPSIWNQLGPENFANLKKLATKFGSGAPKADGEAGDESAVGAGPAAADEDDVPELVGNFDGSS 164
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A267EHA7 (Transcription factor BTF3 (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006052g2 PE=3 SV=1) HSP 1 Score: 163.31 bits (412), Expect = 1.724e-47 Identity = 90/159 (56.60%), Postives = 115/159 (72.33%), Query Frame = 3 Query: 78 RLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-----RENIENKSTSQIN------EDDDVPTLVENFDEQS 521 +++KL D++RIGGKGSARRKKKV+ KNN +DE+K+ TTLK+L V+ IP IEEVNMFKD G +IHF NPKVQASLQ NT +ISGQSE+KNL+E+LPS+W QLGP N N+KK+A + + + E S + ++DDVP LV NFD S Sbjct: 12 KIDKLKAQVDSIRIGGKGSARRKKKVIHKNNATDERKVTTTLKKLAVSSIPGIEEVNMFKDSGEVIHFNNPKVQASLQSNTFAISGQSEVKNLTELLPSIWNQLGPENFANLKKLATKFGSGAPKADGEAGDESAVGAGPAAADEDDVPELVGNFDGSS 170
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Match: btf3 (basic transcription factor 3 [Source:NCBI gene;Acc:103023438]) HSP 1 Score: 154.451 bits (389), Expect = 1.153e-47 Identity = 84/150 (56.00%), Postives = 108/150 (72.00%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQI---NEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLPS+ QLG ++ +++K+AE + + + +EDD+VP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRKLAEALPKQAGDGKAPMAGGEDEDDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:393667]) HSP 1 Score: 150.214 bits (378), Expect = 1.268e-44 Identity = 89/151 (58.94%), Postives = 112/151 (74.17%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++KIAE+ R+ ++N K+ EDDDVP LVENFDE SK Sbjct: 122 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKIAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 272
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Sea Lamprey
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731]) HSP 1 Score: 58.5362 bits (140), Expect = 4.631e-12 Identity = 39/73 (53.42%), Postives = 47/73 (64.38%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDD----GTLIHFKNP 290 EKL KL VRIGGKG+ARRKKKVV + +D+KKLQ++LK+L VN I IEE D G + F NP Sbjct: 20 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNSINGIEEYVFSVCDSPGQGQCLKFNNP 92
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Match: EGD1 (Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication [Source:SGD;Acc:S000005958]) HSP 1 Score: 89.3521 bits (220), Expect = 2.710e-23 Identity = 52/151 (34.44%), Postives = 80/151 (52.98%), Query Frame = 3 Query: 66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVEN--FD 512 +++++L KL KLS N ++GG KK + D+ KLQ+ L +L I + E N FKDDG ++HF VQ + Q NT G + KNL ++ P + QLGP IQ + ++A +M ++ +D+ +P LVE FD Sbjct: 3 IDQEKLAKLQKLSANNKVGGTRRKLNKKAGSSAGANKDDTKLQSQLAKLHAVTIDNVAEANFFKDDGKVMHFNKVGVQVAAQHNTSVFYGLPQEKNLQDLFPGIISQLGPEAIQALSQLAAQMEKHEAKAPADAEKKDEAIPELVEGQTFD 153
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Match: BTT1 (Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication [Source:SGD;Acc:S000002660]) HSP 1 Score: 72.0182 bits (175), Expect = 1.070e-16 Identity = 48/132 (36.36%), Postives = 71/132 (53.79%), Query Frame = 3 Query: 66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKST 461 V++++L KLHKLS ++GG KK + NN D KLQ L +L I + E N FK +G ++HF + VQ + QCN I GQ + L+ + PSV QLG ++ + +A N+EN+ T Sbjct: 3 VDQEKLAKLHKLSAANKVGGTRRKINKKGNLYNNNDKDNTKLQAELHKLHPMTIENVAEANFFKKNGKVLHFNSAVVQIAPQCNLTMIHGQPKENTLNGLYPSVASQLGSQELEYLTGLA----HNLENEQT 130
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Nematostella
Match: EDO45155 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTW0]) HSP 1 Score: 158.303 bits (399), Expect = 1.727e-49 Identity = 83/150 (55.33%), Postives = 113/150 (75.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDDVPTLVENFDEQSKLQ 530 EKL KL VRIGGKG+ARRKKKV+ + +D+KKLQ TLK+L VNPIP IEEVNM K+DGT+IHF NPKVQASL NT +++G +E K+++EMLP + QLG ++ N++++AE+ + + + + +EDD+VP LVENFDE SK + Sbjct: 4 EKLAKLQKEVRIGGKGTARRKKKVLHRTATTDDKKLQNTLKKLSVNPIPGIEEVNMIKEDGTVIHFNNPKVQASLGANTFAVNGHAETKSITEMLPGILNQLGGDSLTNLQRLAEKFPPPEMGSGAVNAEDEDDEVPELVENFDEPSKTE 153
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: btf3 (basic transcription factor 3 [Source:NCBI gene;Acc:101157713]) HSP 1 Score: 155.606 bits (392), Expect = 3.861e-48 Identity = 85/150 (56.67%), Postives = 110/150 (73.33%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKGSARRKKKVV + +D+KKLQ +LK+LGVN I IEEVNMF + GT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++++AE + ++ + K+ + EDDDVP LVENFDE SK Sbjct: 9 EKLAKLQAQVRIGGKGSARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPGILNQLGADSLTSLRRLAEALPKQAADGKAPIAAVEEEDDDVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:NCBI gene;Acc:101173410]) HSP 1 Score: 150.214 bits (378), Expect = 4.643e-46 Identity = 83/148 (56.08%), Postives = 104/148 (70.27%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENK-STSQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV K +D+KKLQ +LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++K+AE+ E S+++ P LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHKTATADDKKLQGSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLSSLRKLAEQFPRQCERPLHASRLSAPKHRPDLVENFDEASK 151
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:NCBI gene;Acc:101173410]) HSP 1 Score: 149.828 bits (377), Expect = 6.593e-46 Identity = 88/152 (57.89%), Postives = 113/152 (74.34%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST----SQINEDDDVPTLVENFDEQSK 524 EKL KL VRIGGKG+ARRKKKVV K +D+KKLQ +LK+L VN I IEEVNM KDDGT+IHF NPKVQASL NT +I+G +E K L+EMLP + QLG ++ +++K+AE+ R++++ K+ ++ EDDDVP LVENFDE SK Sbjct: 4 EKLAKLQAQVRIGGKGTARRKKKVVHKTATADDKKLQGSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLSSLRKLAEQFPRQSMDMKTVKEENAEEEEDDDVPDLVENFDEASK 155
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: ENSORLT00000027651.1 (transcription factor BTF3 [Source:NCBI gene;Acc:101169115]) HSP 1 Score: 137.117 bits (344), Expect = 7.299e-41 Identity = 76/150 (50.67%), Postives = 105/150 (70.00%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINE---DDDVPTLVENFDEQSK 524 EKL KL VRI GKGSARRK+KVV + +D+KKL +LK+LGVN I IEEV+MF + GT+IHF NPKVQASL NT +++G +E K L+EMLP + QLG ++ +++++AE + + + ++ +D+VP LVENFDE SK Sbjct: 9 EKLVKLQAQVRIDGKGSARRKRKVVHRTATADDKKLHFSLKKLGVNNISGIEEVSMFTNQGTVIHFNNPKVQASLAANTFAVTGHAENKQLTEMLPGILSQLGADSLTSLRRLAEHLPKTVGEHKVPIVDAEEEEDEVPDLVENFDEASK 158
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: ENSORLT00000002626.2 (transcription factor BTF3 [Source:NCBI gene;Acc:101169115]) HSP 1 Score: 132.494 bits (332), Expect = 2.978e-39 Identity = 74/147 (50.34%), Postives = 100/147 (68.03%), Query Frame = 3 Query: 84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524 EKL KL VRI GKGSARRK+KVV + +D+KKL +LK+LGVN I IEEV+MF + GT+IHF NPKVQASL NT +++G +E K L+EMLP + QLG ++ +++++AE + + +T + LVENFDE SK Sbjct: 9 EKLVKLQAQVRIDGKGSARRKRKVVHRTATADDKKLHFSLKKLGVNNISGIEEVSMFTNQGTVIHFNNPKVQASLAANTFAVTGHAENKQLTEMLPGILSQLGADSLTSLRRLAEHLPKTGTYHNT--------LVNLVENFDEASK 147
BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Match: SMESG000026176.1 (SMESG000026176.1) HSP 1 Score: 300.442 bits (768), Expect = 2.434e-105 Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 3 Query: 51 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ 530 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ Sbjct: 1 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ 160
BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Match: SMESG000008919.1 (SMESG000008919.1) HSP 1 Score: 168.703 bits (426), Expect = 2.075e-53 Identity = 95/162 (58.64%), Postives = 128/162 (79.01%), Query Frame = 3 Query: 51 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKST--SQINEDDDVPTLVENFDEQSKLQ 530 M+ K+N ++LEKL KLS+NVR GGKG+ RRKKKV+ K++ +D+KKL+ T K L ++P+P +EEVNMFKDDGT+I+F NPKV SL CNT+S++G+SEIK L+E+LP VWQQLGPL KKI+ +N+EN++T +EDDDVPTLV +FD+QSKL+ Sbjct: 1 MDGFKINTEKLEKLSKLSENVRTGGKGTMRRKKKVIVKSSAADDKKLEATFKSLSMSPVP-VEEVNMFKDDGTVINFVNPKVNVSLPCNTLSVTGKSEIKQLTEILPKVWQQLGPLKGDLFKKIS----QNMENQATVEDIDDEDDDVPTLVTDFDQQSKLE 157 The following BLAST results are available for this feature:
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033402 ID=SMED30033402|Name=Nascent polypeptide-associated complex subunit beta|organism=Schmidtea mediterranea sexual|type=transcript|length=620bpback to top protein sequence of SMED30033402-orf-1 >SMED30033402-orf-1 ID=SMED30033402-orf-1|Name=SMED30033402-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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