Nascent polypeptide-associated complex subunit beta

Overview
NameNascent polypeptide-associated complex subunit beta
Smed IDSMED30033402
Length (bp)620
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nascent polypeptide-associated complex subunit beta (SMED30033402) t-SNE clustered cells

Violin plots show distribution of expression levels for Nascent polypeptide-associated complex subunit beta (SMED30033402) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nascent polypeptide-associated complex subunit beta (SMED30033402) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nascent polypeptide-associated complex subunit beta (SMED30033402) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033402

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30033402SMESG000026176.1 dd_Smed_v4_358_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30033402SMESG000026176.1 dd_Smed_v6_358_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30033402SMESG000026176.1 dd_Smed_v6_358_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125])

HSP 1 Score: 157.918 bits (398), Expect = 1.145e-48
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125])

HSP 1 Score: 157.147 bits (396), Expect = 8.227e-48
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:   53 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 202          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:HGNC Symbol;Acc:HGNC:30547])

HSP 1 Score: 147.902 bits (372), Expect = 7.100e-45
Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K+        EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:HGNC Symbol;Acc:HGNC:30547])

HSP 1 Score: 134.806 bits (338), Expect = 6.241e-40
Identity = 71/126 (56.35%), Postives = 97/126 (76.98%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS 458
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K+
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKA 129          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Match: BTF3 (basic transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:1125])

HSP 1 Score: 92.0485 bits (227), Expect = 6.310e-24
Identity = 46/97 (47.42%), Postives = 62/97 (63.92%), Query Frame = 3
Query:  234 IEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524
            IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +                  LVENFDE SK
Sbjct:    2 IEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQY----------------LVENFDEASK 82          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Match: icd-1 (Transcription factor BTF3 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18885])

HSP 1 Score: 140.584 bits (353), Expect = 1.967e-42
Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 3
Query:   75 DRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524
            +R++KL    ++VRIGGKG+ RRKKKV+ K   +D+KKLQ+ LK+L V  IP IEEVNM KDDGT+IHF NPKVQ S+  NT S++G ++ K ++EMLP +  QLGP ++ ++KK+A     N+         ED+DVP LV +FD  SK
Sbjct:    8 ERIKKLQAQQEHVRIGGKGTPRRKKKVIHKTAAADDKKLQSNLKKLSVTNIPGIEEVNMIKDDGTVIHFNNPKVQTSVPANTFSVTGSADNKQITEMLPGILNQLGPESLTHLKKLA----NNVTKLGPDGKGEDEDVPELVGDFDAASK 153          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Match: icd-1 (Transcription factor BTF3 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18885])

HSP 1 Score: 140.584 bits (353), Expect = 1.967e-42
Identity = 76/150 (50.67%), Postives = 104/150 (69.33%), Query Frame = 3
Query:   75 DRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524
            +R++KL    ++VRIGGKG+ RRKKKV+ K   +D+KKLQ+ LK+L V  IP IEEVNM KDDGT+IHF NPKVQ S+  NT S++G ++ K ++EMLP +  QLGP ++ ++KK+A     N+         ED+DVP LV +FD  SK
Sbjct:    8 ERIKKLQAQQEHVRIGGKGTPRRKKKVIHKTAAADDKKLQSNLKKLSVTNIPGIEEVNMIKDDGTVIHFNNPKVQTSVPANTFSVTGSADNKQITEMLPGILNQLGPESLTHLKKLA----NNVTKLGPDGKGEDEDVPELVGDFDAASK 153          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: bic (gene:FBgn0000181 transcript:FBtr0087782)

HSP 1 Score: 135.191 bits (339), Expect = 5.293e-40
Identity = 79/148 (53.38%), Postives = 102/148 (68.92%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM----RENIENKSTSQINEDDDVPTLVENFDE 515
            EKL KL   VRIGGKG+ RRKKK+V     +D+KKLQ++LK+L VN IP IEEVN+ K+DGT+IHF NPK QASL  NT +I+G  E K ++EM+P +  QLGP +I  +KK+A E+           S++    DDDVP LVENF+E
Sbjct:    4 EKLKKLQAQVRIGGKGTPRRKKKIVHSTPATDDKKLQSSLKKLSVNTIPGIEEVNIIKNDGTVIHFNNPKAQASLPTNTFAITGHGENKTITEMVPGILTQLGPQDINQLKKLATEIASKSGAGGAAGSSAADAGDDDVPDLVENFEE 151          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: bic (gene:FBgn0000181 transcript:FBtr0087783)

HSP 1 Score: 135.191 bits (339), Expect = 5.293e-40
Identity = 79/148 (53.38%), Postives = 102/148 (68.92%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM----RENIENKSTSQINEDDDVPTLVENFDE 515
            EKL KL   VRIGGKG+ RRKKK+V     +D+KKLQ++LK+L VN IP IEEVN+ K+DGT+IHF NPK QASL  NT +I+G  E K ++EM+P +  QLGP +I  +KK+A E+           S++    DDDVP LVENF+E
Sbjct:    4 EKLKKLQAQVRIGGKGTPRRKKKIVHSTPATDDKKLQSSLKKLSVNTIPGIEEVNIIKNDGTVIHFNNPKAQASLPTNTFAITGHGENKTITEMVPGILTQLGPQDINQLKKLATEIASKSGAGGAAGSSAADAGDDDVPDLVENFEE 151          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0332341)

HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34
Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3
Query:   81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530
            +EKL +L   VRIGGKG+ RRKKKV+ +   +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL  NT +++G  E + + EMLP +  QLG   +  ++  A  M        +        EDDDVP LV +FDE +K++
Sbjct:    3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0332342)

HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34
Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3
Query:   81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530
            +EKL +L   VRIGGKG+ RRKKKV+ +   +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL  NT +++G  E + + EMLP +  QLG   +  ++  A  M        +        EDDDVP LV +FDE +K++
Sbjct:    3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Match: CG11835 (gene:FBgn0031264 transcript:FBtr0078076)

HSP 1 Score: 128.257 bits (321), Expect = 5.078e-34
Identity = 72/154 (46.75%), Postives = 99/154 (64.29%), Query Frame = 3
Query:   81 LEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTS----QINEDDDVPTLVENFDEQSKLQ 530
            +EKL +L   VRIGGKG+ RRKKKV+ +   +D+KKLQ++LK+L V+ IP IEEVN+ KDD T+IHF NPK QASL  NT +++G  E + + EMLP +  QLG   +  ++  A  M        +        EDDDVP LV +FDE +K++
Sbjct:    3 VEKLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDLTVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQKGAPGSGDGPLPAEEDDDVPLLVGDFDEVAKVE 156          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731])

HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + ++  + K+     E  DD+VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731])

HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + ++  + K+     E  DD+VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731])

HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + ++  + K+     E  DD+VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731])

HSP 1 Score: 151.754 bits (382), Expect = 1.411e-46
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINE--DDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + ++  + K+     E  DD+VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQAGDGKAPVAGGEEDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:393667])

HSP 1 Score: 150.599 bits (379), Expect = 3.585e-46
Identity = 88/151 (58.28%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++K+AE+  R+ ++N   K+     EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLVVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: ttc34 (tetratricopeptide repeat domain 34 [Source:Xenbase;Acc:XB-GENE-991680])

HSP 1 Score: 154.066 bits (388), Expect = 1.065e-46
Identity = 85/150 (56.67%), Postives = 113/150 (75.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+   S   EDD+VP LVENFDE SK
Sbjct:   56 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLASGEEEDDEVPELVENFDEASK 205          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:496686])

HSP 1 Score: 149.058 bits (375), Expect = 2.242e-45
Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E+K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K++       EDDDVP LV NFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Match: ttc34 (tetratricopeptide repeat domain 34 [Source:Xenbase;Acc:XB-GENE-991680])

HSP 1 Score: 140.584 bits (353), Expect = 2.089e-41
Identity = 82/169 (48.52%), Postives = 116/169 (68.64%), Query Frame = 3
Query:   27 LNLFIIKYMESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524
            LN      + +V + K R  +   L   V + G+G+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+   S   EDD+VP LVENFDE SK
Sbjct:   32 LNPLCSPGVPAVCLQKVR-GRYRVLGGCVWVSGQGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLASGEEEDDEVPELVENFDEASK 199          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875])

HSP 1 Score: 157.918 bits (398), Expect = 7.863e-49
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875])

HSP 1 Score: 157.918 bits (398), Expect = 7.863e-49
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3 (basic transcription factor 3 [Source:MGI Symbol;Acc:MGI:1202875])

HSP 1 Score: 156.762 bits (395), Expect = 5.807e-48
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:   51 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 200          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3l4 (basic transcription factor 3-like 4 [Source:MGI Symbol;Acc:MGI:1915312])

HSP 1 Score: 147.902 bits (372), Expect = 4.875e-45
Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K+        EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Match: Btf3l4 (basic transcription factor 3-like 4 [Source:MGI Symbol;Acc:MGI:1915312])

HSP 1 Score: 147.902 bits (372), Expect = 4.875e-45
Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS---TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K+        EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKAPKPEDIDEEDDDVPDLVENFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|P20290|BTF3_HUMAN (Transcription factor BTF3 OS=Homo sapiens OX=9606 GN=BTF3 PE=1 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 3.951e-47
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:   53 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 202          
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q64152|BTF3_MOUSE (Transcription factor BTF3 OS=Mus musculus OX=10090 GN=Btf3 PE=1 SV=3)

HSP 1 Score: 156.762 bits (395), Expect = 4.062e-47
Identity = 85/150 (56.67%), Postives = 112/150 (74.67%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDD--VPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++++AE + +++++ K+     EDDD  VP LVENFDE SK
Sbjct:   51 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASK 200          
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q6PC91|BT3L4_DANRE (Transcription factor BTF3 homolog 4 OS=Danio rerio OX=7955 GN=btf3l4 PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 2.706e-45
Identity = 88/151 (58.28%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++K+AE+  R+ ++N   K+     EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLVVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q5M8V0|BT3L4_XENTR (Transcription factor BTF3 homolog 4 OS=Xenopus tropicalis OX=8364 GN=btf3l4 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.188e-44
Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E+K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K++       EDDDVP LV NFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Match: sp|Q4KLF5|BT3L4_XENLA (Transcription factor BTF3 homolog 4 OS=Xenopus laevis OX=8355 GN=btf3l4 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.188e-44
Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST---SQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E+K ++EMLP +  QLG  ++ +++K+AE+  R+ +++K++       EDDDVP LV NFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAEVKQITEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKASKPEDIEEEDDDVPELVGNFDEASK 154          
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A2R7WAG3 (Transcription factor BTF3 OS=Oncopeltus fasciatus OX=7536 GN=OFAS_OFAS006791 PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 5.509e-48
Identity = 85/147 (57.82%), Postives = 105/147 (71.43%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ RRKKKV+     +D+KKLQ++LK+L VN IP IEEVNM KDDGT+IHF NPK QASL  NT +++G  E K ++EMLP +  QLGP  I  +K++A  +  N   K+T  I EDD+VP LVENFDE SK
Sbjct:    4 EKLKKLQAQVRIGGKGTPRRKKKVLHATAATDDKKLQSSLKKLSVNTIPGIEEVNMIKDDGTVIHFNNPKAQASLAANTFAVTGHGETKQITEMLPGILSQLGPEGINQLKRLATSVAGNTAGKAT--IEEDDEVPDLVENFDEASK 148          
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: B5XE39 (Transcription factor BTF3 OS=Salmo salar OX=8030 GN=BT3L4 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.224e-47
Identity = 88/150 (58.67%), Postives = 113/150 (75.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKGSARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDG++IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++K+AE+  R+ +++K+     I EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGSARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGSVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKLAEQFPRQVLDSKTPKAEDIEEDDDVPDLVENFDEASK 153          
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: C3YIK9 (Transcription factor BTF3 OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_131258 PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.417e-47
Identity = 85/157 (54.14%), Postives = 116/157 (73.89%), Query Frame = 3
Query:   66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRE----NIENKSTSQINEDDDVPTLVENFDEQSK 524
            +N+++L ++ K+S+ VRIGGKGSARRK+KVV +   +D+KKLQ++LK+L VN IP IEEVNM +DDGT++HF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ N+KK+AE +      +  +  T+   EDDDVP LV NFDE SK
Sbjct:    1 MNQEKLNRMQKMSEQVRIGGKGSARRKRKVVHRTATTDDKKLQSSLKKLTVNNIPGIEEVNMIRDDGTVVHFNNPKVQASLAANTFAITGHAENKQLTEMLPGILNQLGADSLSNLKKLAESLPSQDFFSPFSAPTATAGEDDDVPELVANFDEPSK 157          
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A1I8GPL3 (Transcription factor BTF3 OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.579e-47
Identity = 90/159 (56.60%), Postives = 115/159 (72.33%), Query Frame = 3
Query:   78 RLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-----RENIENKSTSQIN------EDDDVPTLVENFDEQS 521
            +++KL    D++RIGGKGSARRKKKV+ KNN +DE+K+ TTLK+L V+ IP IEEVNMFKD G +IHF NPKVQASLQ NT +ISGQSE+KNL+E+LPS+W QLGP N  N+KK+A +      + + E    S +       ++DDVP LV NFD  S
Sbjct:    6 KIDKLKAQVDSIRIGGKGSARRKKKVIHKNNATDERKVTTTLKKLAVSSIPGIEEVNMFKDSGEVIHFNNPKVQASLQSNTFAISGQSEVKNLTELLPSIWNQLGPENFANLKKLATKFGSGAPKADGEAGDESAVGAGPAAADEDDVPELVGNFDGSS 164          
BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Match: A0A267EHA7 (Transcription factor BTF3 (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006052g2 PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.724e-47
Identity = 90/159 (56.60%), Postives = 115/159 (72.33%), Query Frame = 3
Query:   78 RLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-----RENIENKSTSQIN------EDDDVPTLVENFDEQS 521
            +++KL    D++RIGGKGSARRKKKV+ KNN +DE+K+ TTLK+L V+ IP IEEVNMFKD G +IHF NPKVQASLQ NT +ISGQSE+KNL+E+LPS+W QLGP N  N+KK+A +      + + E    S +       ++DDVP LV NFD  S
Sbjct:   12 KIDKLKAQVDSIRIGGKGSARRKKKVIHKNNATDERKVTTTLKKLAVSSIPGIEEVNMFKDSGEVIHFNNPKVQASLQSNTFAISGQSEVKNLTELLPSIWNQLGPENFANLKKLATKFGSGAPKADGEAGDESAVGAGPAAADEDDVPELVGNFDGSS 170          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Match: btf3 (basic transcription factor 3 [Source:NCBI gene;Acc:103023438])

HSP 1 Score: 154.451 bits (389), Expect = 1.153e-47
Identity = 84/150 (56.00%), Postives = 108/150 (72.00%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQI---NEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLPS+  QLG  ++ +++K+AE + +   +         +EDD+VP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRKLAEALPKQAGDGKAPMAGGEDEDDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Match: btf3l4 (basic transcription factor 3-like 4 [Source:NCBI gene;Acc:393667])

HSP 1 Score: 150.214 bits (378), Expect = 1.268e-44
Identity = 89/151 (58.94%), Postives = 112/151 (74.17%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIEN---KSTSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++KIAE+  R+ ++N   K+     EDDDVP LVENFDE SK
Sbjct:  122 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLTSLRKIAEQFPRQVLDNKAPKAEDIDEEDDDVPDLVENFDEASK 272          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Sea Lamprey
Match: btf3 (basic transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8731])

HSP 1 Score: 58.5362 bits (140), Expect = 4.631e-12
Identity = 39/73 (53.42%), Postives = 47/73 (64.38%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDD----GTLIHFKNP 290
            EKL KL   VRIGGKG+ARRKKKVV +   +D+KKLQ++LK+L VN I  IEE      D    G  + F NP
Sbjct:   20 EKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNSINGIEEYVFSVCDSPGQGQCLKFNNP 92          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Match: EGD1 (Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication [Source:SGD;Acc:S000005958])

HSP 1 Score: 89.3521 bits (220), Expect = 2.710e-23
Identity = 52/151 (34.44%), Postives = 80/151 (52.98%), Query Frame = 3
Query:   66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVEN--FD 512
            +++++L KL KLS N ++GG      KK   +     D+ KLQ+ L +L    I  + E N FKDDG ++HF    VQ + Q NT    G  + KNL ++ P +  QLGP  IQ + ++A +M ++          +D+ +P LVE   FD
Sbjct:    3 IDQEKLAKLQKLSANNKVGGTRRKLNKKAGSSAGANKDDTKLQSQLAKLHAVTIDNVAEANFFKDDGKVMHFNKVGVQVAAQHNTSVFYGLPQEKNLQDLFPGIISQLGPEAIQALSQLAAQMEKHEAKAPADAEKKDEAIPELVEGQTFD 153          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Match: BTT1 (Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication [Source:SGD;Acc:S000002660])

HSP 1 Score: 72.0182 bits (175), Expect = 1.070e-16
Identity = 48/132 (36.36%), Postives = 71/132 (53.79%), Query Frame = 3
Query:   66 VNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKST 461
            V++++L KLHKLS   ++GG      KK  +  NN  D  KLQ  L +L    I  + E N FK +G ++HF +  VQ + QCN   I GQ +   L+ + PSV  QLG   ++ +  +A     N+EN+ T
Sbjct:    3 VDQEKLAKLHKLSAANKVGGTRRKINKKGNLYNNNDKDNTKLQAELHKLHPMTIENVAEANFFKKNGKVLHFNSAVVQIAPQCNLTMIHGQPKENTLNGLYPSVASQLGSQELEYLTGLA----HNLENEQT 130          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Nematostella
Match: EDO45155 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTW0])

HSP 1 Score: 158.303 bits (399), Expect = 1.727e-49
Identity = 83/150 (55.33%), Postives = 113/150 (75.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKSTSQINEDDDVPTLVENFDEQSKLQ 530
            EKL KL   VRIGGKG+ARRKKKV+ +   +D+KKLQ TLK+L VNPIP IEEVNM K+DGT+IHF NPKVQASL  NT +++G +E K+++EMLP +  QLG  ++ N++++AE+     + + + +  +EDD+VP LVENFDE SK +
Sbjct:    4 EKLAKLQKEVRIGGKGTARRKKKVLHRTATTDDKKLQNTLKKLSVNPIPGIEEVNMIKEDGTVIHFNNPKVQASLGANTFAVNGHAETKSITEMLPGILNQLGGDSLTNLQRLAEKFPPPEMGSGAVNAEDEDDEVPELVENFDEPSKTE 153          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: btf3 (basic transcription factor 3 [Source:NCBI gene;Acc:101157713])

HSP 1 Score: 155.606 bits (392), Expect = 3.861e-48
Identity = 85/150 (56.67%), Postives = 110/150 (73.33%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKS--TSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKGSARRKKKVV +   +D+KKLQ +LK+LGVN I  IEEVNMF + GT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++++AE + ++  + K+   +   EDDDVP LVENFDE SK
Sbjct:    9 EKLAKLQAQVRIGGKGSARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPGILNQLGADSLTSLRRLAEALPKQAADGKAPIAAVEEEDDDVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:NCBI gene;Acc:101173410])

HSP 1 Score: 150.214 bits (378), Expect = 4.643e-46
Identity = 83/148 (56.08%), Postives = 104/148 (70.27%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENK-STSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV K   +D+KKLQ +LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++K+AE+     E     S+++     P LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHKTATADDKKLQGSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLSSLRKLAEQFPRQCERPLHASRLSAPKHRPDLVENFDEASK 151          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: BTF3L4 (basic transcription factor 3 like 4 [Source:NCBI gene;Acc:101173410])

HSP 1 Score: 149.828 bits (377), Expect = 6.593e-46
Identity = 88/152 (57.89%), Postives = 113/152 (74.34%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEM-RENIENKST----SQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRIGGKG+ARRKKKVV K   +D+KKLQ +LK+L VN I  IEEVNM KDDGT+IHF NPKVQASL  NT +I+G +E K L+EMLP +  QLG  ++ +++K+AE+  R++++ K+     ++  EDDDVP LVENFDE SK
Sbjct:    4 EKLAKLQAQVRIGGKGTARRKKKVVHKTATADDKKLQGSLKKLAVNNIAGIEEVNMIKDDGTVIHFNNPKVQASLSANTFAITGHAETKQLTEMLPGILSQLGADSLSSLRKLAEQFPRQSMDMKTVKEENAEEEEDDDVPDLVENFDEASK 155          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: ENSORLT00000027651.1 (transcription factor BTF3 [Source:NCBI gene;Acc:101169115])

HSP 1 Score: 137.117 bits (344), Expect = 7.299e-41
Identity = 76/150 (50.67%), Postives = 105/150 (70.00%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINE---DDDVPTLVENFDEQSK 524
            EKL KL   VRI GKGSARRK+KVV +   +D+KKL  +LK+LGVN I  IEEV+MF + GT+IHF NPKVQASL  NT +++G +E K L+EMLP +  QLG  ++ +++++AE + + +       ++    +D+VP LVENFDE SK
Sbjct:    9 EKLVKLQAQVRIDGKGSARRKRKVVHRTATADDKKLHFSLKKLGVNNISGIEEVSMFTNQGTVIHFNNPKVQASLAANTFAVTGHAENKQLTEMLPGILSQLGADSLTSLRRLAEHLPKTVGEHKVPIVDAEEEEDEVPDLVENFDEASK 158          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Match: ENSORLT00000002626.2 (transcription factor BTF3 [Source:NCBI gene;Acc:101169115])

HSP 1 Score: 132.494 bits (332), Expect = 2.978e-39
Identity = 74/147 (50.34%), Postives = 100/147 (68.03%), Query Frame = 3
Query:   84 EKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSK 524
            EKL KL   VRI GKGSARRK+KVV +   +D+KKL  +LK+LGVN I  IEEV+MF + GT+IHF NPKVQASL  NT +++G +E K L+EMLP +  QLG  ++ +++++AE + +     +T        +  LVENFDE SK
Sbjct:    9 EKLVKLQAQVRIDGKGSARRKRKVVHRTATADDKKLHFSLKKLGVNNISGIEEVSMFTNQGTVIHFNNPKVQASLAANTFAVTGHAENKQLTEMLPGILSQLGADSLTSLRRLAEHLPKTGTYHNT--------LVNLVENFDEASK 147          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Match: SMESG000026176.1 (SMESG000026176.1)

HSP 1 Score: 300.442 bits (768), Expect = 2.434e-105
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 3
Query:   51 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ 530
            MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ
Sbjct:    1 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLVENFDEQSKLQ 160          
BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Match: SMESG000008919.1 (SMESG000008919.1)

HSP 1 Score: 168.703 bits (426), Expect = 2.075e-53
Identity = 95/162 (58.64%), Postives = 128/162 (79.01%), Query Frame = 3
Query:   51 MESVKVNKDRLEKLHKLSDNVRIGGKGSARRXXXXXXXXXXSDEKKLQTTLKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIKNLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKST--SQINEDDDVPTLVENFDEQSKLQ 530
            M+  K+N ++LEKL KLS+NVR GGKG+ RRKKKV+ K++ +D+KKL+ T K L ++P+P +EEVNMFKDDGT+I+F NPKV  SL CNT+S++G+SEIK L+E+LP VWQQLGPL     KKI+    +N+EN++T     +EDDDVPTLV +FD+QSKL+
Sbjct:    1 MDGFKINTEKLEKLSKLSENVRTGGKGTMRRKKKVIVKSSAADDKKLEATFKSLSMSPVP-VEEVNMFKDDGTVINFVNPKVNVSLPCNTLSVTGKSEIKQLTEILPKVWQQLGPLKGDLFKKIS----QNMENQATVEDIDDEDDDVPTLVTDFDQQSKLE 157          
The following BLAST results are available for this feature:
BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BTF31.145e-4856.67basic transcription factor 3 [Source:HGNC Symbol;A... [more]
BTF38.227e-4856.67basic transcription factor 3 [Source:HGNC Symbol;A... [more]
BTF3L47.100e-4556.95basic transcription factor 3 like 4 [Source:HGNC S... [more]
BTF3L46.241e-4056.35basic transcription factor 3 like 4 [Source:HGNC S... [more]
BTF36.310e-2447.42basic transcription factor 3 [Source:HGNC Symbol;A... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
icd-11.967e-4250.67Transcription factor BTF3 homolog [Source:UniProt... [more]
icd-11.967e-4250.67Transcription factor BTF3 homolog [Source:UniProt... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
bic5.293e-4053.38gene:FBgn0000181 transcript:FBtr0087782[more]
bic5.293e-4053.38gene:FBgn0000181 transcript:FBtr0087783[more]
CG118355.078e-3446.75gene:FBgn0031264 transcript:FBtr0332341[more]
CG118355.078e-3446.75gene:FBgn0031264 transcript:FBtr0332342[more]
CG118355.078e-3446.75gene:FBgn0031264 transcript:FBtr0078076[more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
btf31.411e-4656.00basic transcription factor 3 [Source:ZFIN;Acc:ZDB-... [more]
btf31.411e-4656.00basic transcription factor 3 [Source:ZFIN;Acc:ZDB-... [more]
btf31.411e-4656.00basic transcription factor 3 [Source:ZFIN;Acc:ZDB-... [more]
btf31.411e-4656.00basic transcription factor 3 [Source:ZFIN;Acc:ZDB-... [more]
btf3l43.585e-4658.28basic transcription factor 3-like 4 [Source:NCBI g... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ttc341.065e-4656.67tetratricopeptide repeat domain 34 [Source:Xenbase... [more]
btf3l42.242e-4556.29basic transcription factor 3-like 4 [Source:NCBI g... [more]
ttc342.089e-4148.52tetratricopeptide repeat domain 34 [Source:Xenbase... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Btf37.863e-4956.67basic transcription factor 3 [Source:MGI Symbol;Ac... [more]
Btf37.863e-4956.67basic transcription factor 3 [Source:MGI Symbol;Ac... [more]
Btf35.807e-4856.67basic transcription factor 3 [Source:MGI Symbol;Ac... [more]
Btf3l44.875e-4556.95basic transcription factor 3-like 4 [Source:MGI Sy... [more]
Btf3l44.875e-4556.95basic transcription factor 3-like 4 [Source:MGI Sy... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20290|BTF3_HUMAN3.951e-4756.67Transcription factor BTF3 OS=Homo sapiens OX=9606 ... [more]
sp|Q64152|BTF3_MOUSE4.062e-4756.67Transcription factor BTF3 OS=Mus musculus OX=10090... [more]
sp|Q6PC91|BT3L4_DANRE2.706e-4558.28Transcription factor BTF3 homolog 4 OS=Danio rerio... [more]
sp|Q5M8V0|BT3L4_XENTR1.188e-4456.29Transcription factor BTF3 homolog 4 OS=Xenopus tro... [more]
sp|Q4KLF5|BT3L4_XENLA1.188e-4456.29Transcription factor BTF3 homolog 4 OS=Xenopus lae... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2R7WAG35.509e-4857.82Transcription factor BTF3 OS=Oncopeltus fasciatus ... [more]
B5XE391.224e-4758.67Transcription factor BTF3 OS=Salmo salar OX=8030 G... [more]
C3YIK91.417e-4754.14Transcription factor BTF3 OS=Branchiostoma florida... [more]
A0A1I8GPL31.579e-4756.60Transcription factor BTF3 OS=Macrostomum lignano O... [more]
A0A267EHA71.724e-4756.60Transcription factor BTF3 (Fragment) OS=Macrostomu... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
btf31.153e-4756.00basic transcription factor 3 [Source:NCBI gene;Acc... [more]
btf3l41.268e-4458.94basic transcription factor 3-like 4 [Source:NCBI g... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
btf34.631e-1253.42basic transcription factor 3 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
EGD12.710e-2334.44Subunit beta1 of the nascent polypeptide-associate... [more]
BTT11.070e-1636.36Heterotrimeric nascent polypeptide-associated comp... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO451551.727e-4955.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
btf33.861e-4856.67basic transcription factor 3 [Source:NCBI gene;Acc... [more]
BTF3L44.643e-4656.08basic transcription factor 3 like 4 [Source:NCBI g... [more]
BTF3L46.593e-4657.89basic transcription factor 3 like 4 [Source:NCBI g... [more]
ENSORLT00000027651.17.299e-4150.67transcription factor BTF3 [Source:NCBI gene;Acc:10... [more]
ENSORLT00000002626.22.978e-3950.34transcription factor BTF3 [Source:NCBI gene;Acc:10... [more]
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BLAST of Nascent polypeptide-associated complex subunit beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000026176.12.434e-105100.00SMESG000026176.1[more]
SMESG000008919.12.075e-5358.64SMESG000008919.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033402 ID=SMED30033402|Name=Nascent polypeptide-associated complex subunit beta|organism=Schmidtea mediterranea sexual|type=transcript|length=620bp
GTAAATGAGACCTTACTAAATTATTTTTGAATTTGTTTATAATAAAATAC
ATGGAAAGTGTGAAAGTTAATAAGGATAGATTAGAGAAACTTCATAAGTT
ATCTGACAATGTACGAATTGGTGGTAAAGGTTCTGCTAGACGAAAGAAGA
AGGTTGTCACGAAAAATAATGTCAGTGATGAAAAGAAGCTGCAAACGACG
CTGAAACGTTTGGGTGTTAATCCTATCCCAACAATTGAAGAAGTGAATAT
GTTTAAGGATGATGGTACGTTGATTCACTTTAAGAATCCAAAAGTACAGG
CTTCGCTACAATGTAATACTATTTCAATATCGGGTCAATCGGAAATTAAA
AATTTAAGTGAAATGCTTCCGAGTGTATGGCAACAATTGGGTCCACTTAA
TATACAAAATATTAAAAAGATTGCTGAAGAAATGCGAGAAAATATTGAAA
ATAAATCAACTTCCCAAATTAATGAAGATGATGATGTCCCAACATTGGTT
GAAAACTTTGATGAACAGAGCAAACTTCAGTAATCTGTAACCCATAATGT
TATTTTTGTTGAAATAATAAATTTGGATAGCATGTCCATTCTTTATTGAA
TAAATTAGAAAAACTTTGTT
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protein sequence of SMED30033402-orf-1

>SMED30033402-orf-1 ID=SMED30033402-orf-1|Name=SMED30033402-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MESVKVNKDRLEKLHKLSDNVRIGGKGSARRKKKVVTKNNVSDEKKLQTT
LKRLGVNPIPTIEEVNMFKDDGTLIHFKNPKVQASLQCNTISISGQSEIK
NLSEMLPSVWQQLGPLNIQNIKKIAEEMRENIENKSTSQINEDDDVPTLV
ENFDEQSKLQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0015031protein transport
GO:0008150biological_process
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000110pharynx progenitor cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR038187NAC_A/B_dom_sf
IPR039370BTF3
IPR002715Nas_poly-pep-assoc_cplx_dom
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..160
IPR002715Nascent polypeptide-associated complex NAC domainSMARTSM01407NAC_2coord: 44..100
e-value: 4.5E-24
score: 96.0
IPR002715Nascent polypeptide-associated complex NAC domainPFAMPF01849NACcoord: 44..99
e-value: 1.5E-22
score: 79.3
IPR002715Nascent polypeptide-associated complex NAC domainPROSITEPS51151NAC_ABcoord: 41..106
score: 17.146
IPR038187NAC A/B domain superfamilyGENE3DG3DSA:2.20.70.30coord: 56..113
e-value: 6.5E-28
score: 98.1
IPR039370Transcription factor BTF3PANTHERPTHR10351TRANSCRIPTION FACTOR BTF3 FAMILY MEMBERcoord: 12..158