DNA replication complex GINS protein SLD5

Overview
NameDNA replication complex GINS protein SLD5
Smed IDSMED30033400
Length (bp)853
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA replication complex GINS protein SLD5 (SMED30033400) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA replication complex GINS protein SLD5 (SMED30033400) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA replication complex GINS protein SLD5 (SMED30033400) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA replication complex GINS protein SLD5 (SMED30033400) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033400

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033400h1SMcG0021345 SmedASXL_000736SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
muscle cellSMED30033400h1SMcG0021345 dd_Smed_v4_23589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30033400h1SMcG0021345 dd_Smed_v4_23589_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Match: GINS4 (GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGNC:28226])

HSP 1 Score: 80.4925 bits (197), Expect = 2.030e-17
Identity = 62/210 (29.52%), Postives = 113/210 (53.81%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E   A  +     ++L +NV  +  P   +  +L + V  P+ D +VF    +  E I V  D +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYL-KNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYV-IDLEKGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Match: GINS4 (GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGNC:28226])

HSP 1 Score: 80.4925 bits (197), Expect = 2.030e-17
Identity = 62/210 (29.52%), Postives = 113/210 (53.81%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E   A  +     ++L +NV  +  P   +  +L + V  P+ D +VF    +  E I V  D +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYL-KNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYV-IDLEKGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Match: GINS4 (GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGNC:28226])

HSP 1 Score: 72.0182 bits (175), Expect = 1.366e-14
Identity = 50/158 (31.65%), Postives = 90/158 (56.96%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVF 544
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E   A  +     ++L +NV  +  P   +  +L + V  P+ D +VF
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYL-KNVALKHMPPNLQKVDLFRAVPKPDLDSYVF 172
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Match: GINS4 (GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGNC:28226])

HSP 1 Score: 58.151 bits (139), Expect = 2.073e-10
Identity = 32/85 (37.65%), Postives = 55/85 (64.71%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKI 352
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ K+
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKV 100
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Match: GINS4 (GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGNC:28226])

HSP 1 Score: 57.7658 bits (138), Expect = 3.648e-10
Identity = 32/84 (38.10%), Postives = 54/84 (64.29%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHK 349
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ K
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMK 99
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Celegans
Match: sld-5 (DNA replication complex GINS protein SLD5 [Source:UniProtKB/TrEMBL;Acc:D9PTP3])

HSP 1 Score: 59.3066 bits (142), Expect = 2.623e-10
Identity = 32/91 (35.16%), Postives = 55/91 (60.44%), Query Frame = 2 
Query:   92 YESDEED--ITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKEL-LAQHILKIHIERIKYIITDYSRTRIHKIE 355
            ++ DE D   T EE+++KM   WQNE  +  LL  Q E++++L+ Q++  EEN      ++ L   + ++ ++RI +I +DY R R+ KIE
Sbjct:   13 FDDDEYDEMTTPEEVLRKMTATWQNELCAPCLLPTQMELVEILLDQIQGMEENIGKQTDKMQLRISVHRVELQRIGFITSDYVRCRLQKIE 103
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Fly
Match: Sld5 (gene:FBgn0039403 transcript:FBtr0084975)

HSP 1 Score: 50.0618 bits (118), Expect = 4.779e-7
Identity = 51/210 (24.29%), Postives = 102/210 (48.57%), Query Frame = 2 
Query:  107 EDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENT-DINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE----APNN--LTPAEKMHATLYQKLISN--HLIENVIFQTNP-RKDFNLSQFVNSPNSDKFVFC---FAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            E IT +++++ ++  W NE  +  +L  Q ++++L+++Q+   EE   D+++ +  A  +  + +ER++YI+  Y R R+ KIE +  + L +     P++  L+P E   A   Q+  SN       V  Q  P ++     Q + +PN    VF     A+  V + V+++         + + AG Q + P++ V   ++     LI
Sbjct:   31 EQITAQKVLEIIETAWINEMCAPEILPSQTDMLELMVSQVAHMEEQMRDLDKNDFRAV-VHSMELERVRYIMASYLRCRLQKIETFTQHILNQEESREPDDKRLSPEETKFA---QEFASNVDEYFHKVATQYMPNQQRGEAEQRIVTPNLMSHVFLKANVAVPAVIVGVDDE--------EVDMAAGSQHIIPYQLVADLIQNNQAQLI 228
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Zebrafish
Match: gins4 (GINS complex subunit 4 (Sld5 homolog) [Source:ZFIN;Acc:ZDB-GENE-040801-59])

HSP 1 Score: 70.0922 bits (170), Expect = 6.628e-14
Identity = 56/211 (26.54%), Postives = 102/211 (48.34%), Query Frame = 2 
Query:   89 LYESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEA-------PNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            L   +EE +T  ELI +++  W NEK S  LLE + E+++ +M QL   EEN    +K  L   + ++ I+RI+++++ + R+R+ KIE++  + L++        P+ L+P E   A  Y      +L    +    P  +  ++ + V  P  D FVF    +  E  + E          + +  G Q +  +R++   V  G++ LI
Sbjct:    7 LSGGEEELMTPAELISRLEEAWLNEKFSPELLENKSELVECVMEQLTHMEENLQRVRKGDLKASVHRMEIDRIRFVLSSFLRSRLKKIEKFFPHVLEKEKSRADGDPSFLSPEEFAFAKEYLANTEVYLRAVALKHMPPNLQSVDMMKAVPEPCLDSFVFLRVKEKQENILVEPETDEQREYVVDLDEGSQHLMRYRTIAPLVASGAVQLI 217
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Zebrafish
Match: gins4 (GINS complex subunit 4 (Sld5 homolog) [Source:ZFIN;Acc:ZDB-GENE-040801-59])

HSP 1 Score: 67.0106 bits (162), Expect = 6.183e-13
Identity = 53/203 (26.11%), Postives = 98/203 (48.28%), Query Frame = 2 
Query:  113 ITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEA-------PNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            +T  ELI +++  W NEK S  LLE + E+++ +M QL   EEN    +K  L   + ++ I+RI+++++ + R+R+ KIE++  + L++        P+ L+P E   A  Y      +L    +    P  +  ++ + V  P  D FVF    +  E  + E          + +  G Q +  +R++   V  G++ LI
Sbjct:    1 MTPAELISRLEEAWLNEKFSPELLENKSELVECVMEQLTHMEENLQRVRKGDLKASVHRMEIDRIRFVLSSFLRSRLKKIEKFFPHVLEKEKSRADGDPSFLSPEEFAFAKEYLANTEVYLRAVALKHMPPNLQSVDMMKAVPEPCLDSFVFLRVKEKQENILVEPETDEQREYVVDLDEGSQHLMRYRTIAPLVASGAVQLI 203
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Xenopus
Match: gins4 (GINS complex subunit 4 (Sld5 homolog) [Source:NCBI gene;Acc:496455])

HSP 1 Score: 73.1738 bits (178), Expect = 8.367e-15
Identity = 64/213 (30.05%), Postives = 103/213 (48.36%), Query Frame = 2 
Query:  101 DEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMK-----LVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
             EE +T  ELI K++  W NEK    LLE + EI++ +M QL   E+N    +   L      + IERI+Y+++ Y R+R+ KIE++  + L++        P +L+P E   A  Y    +  L+++V  +  P   +  +L + V  PN D FVF    +     LVE   +E      DM       G Q +  +R++   V  G++ LI
Sbjct:   15 GEEVLTPAELINKLEEAWLNEKFGPELLESKSEIVECVMEQLSHMEQNLRRARPGDLKISFHHMEIERIRYMLSSYLRSRMLKIEKFFPHILEKEKSRGEGEPPHLSPEEFAFAKEYMT-NTETLLKSVALRHMPPNLQTVDLLKCVPKPNLDSFVFLRVKEEQKNILVEPETDEQSEYAIDM-----EVGSQHLIRYRTIAPLVASGAVKLI 221
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Mouse
Match: Gins4 (GINS complex subunit 4 (Sld5 homolog) [Source:MGI Symbol;Acc:MGI:1923847])

HSP 1 Score: 80.8777 bits (198), Expect = 1.039e-17
Identity = 63/210 (30.00%), Postives = 111/210 (52.86%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI+K++  W NEK +  LLE + EI++ +M QL+  EEN    +K  L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E + A  Y      H  +NV  +  P   +  +L + V  P+ D +VF    +  E I V  + +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEMVLTPAELIEKLEQAWMNEKFAPELLESKAEIVECVMEQLEHMEENLRRAKKGDLKVSIHRMEMERIRYVLSSYLRCRLMKIEKFFPHILEKEKVRSEGEPSSLSPEEFVFAKEYMDHTETHF-KNVALKHMPPNLQKVDLLRAVPKPDLDSYVFLRVKERQENILVEPEADEQRDYV-IDLEVGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Match: sp|Q99LZ3|SLD5_MOUSE (DNA replication complex GINS protein SLD5 OS=Mus musculus OX=10090 GN=Gins4 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 7.266e-17
Identity = 63/210 (30.00%), Postives = 111/210 (52.86%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI+K++  W NEK +  LLE + EI++ +M QL+  EEN    +K  L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E + A  Y      H  +NV  +  P   +  +L + V  P+ D +VF    +  E I V  + +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEMVLTPAELIEKLEQAWMNEKFAPELLESKAEIVECVMEQLEHMEENLRRAKKGDLKVSIHRMEMERIRYVLSSYLRCRLMKIEKFFPHILEKEKVRSEGEPSSLSPEEFVFAKEYMDHTETHF-KNVALKHMPPNLQKVDLLRAVPKPDLDSYVFLRVKERQENILVEPEADEQRDYV-IDLEVGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Match: sp|Q9BRT9|SLD5_HUMAN (DNA replication complex GINS protein SLD5 OS=Homo sapiens OX=9606 GN=GINS4 PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 9.748e-17
Identity = 62/210 (29.52%), Postives = 113/210 (53.81%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    ++E L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E   A  +     ++L +NV  +  P   +  +L + V  P+ D +VF    +  E I V  D +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYL-KNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYV-IDLEKGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Match: sp|Q499W2|SLD5_RAT (DNA replication complex GINS protein SLD5 OS=Rattus norvegicus OX=10116 GN=Gins4 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.432e-16
Identity = 62/210 (29.52%), Postives = 111/210 (52.86%), Query Frame = 2 
Query:   98 SDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR--KDFNLSQFVNSPNSDKFVFCFAMKLVE-INVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            S+E  +T  ELI++++  W NEK +  LLE + EI++ +M QL+  EEN    +K  L   I ++ +ERI+Y+++ Y R R+ KIE++  + L++        P++L+P E + A  Y      H  +NV  +  P   +  +L + V  P+ D +VF    +  E I V  + +   D + I +  G Q +  ++++   V  G++ LI
Sbjct:   16 SEEVVLTPAELIERLEQAWMNEKFAPELLESKSEIVECVMEQLEHMEENLRRAKKGDLKVSIHRMEMERIRYVLSSYLRCRLMKIEKFFPHILEKEKTRPAGEPSSLSPEEFVFAKEYMDHTETHF-KNVALKHMPPNLQKVDLMRAVPKPDLDSYVFLRVKERQENILVEPEADEQRDYV-IDLEEGSQHLIRYKTIAPLVASGAVQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Match: sp|A2VE40|SLD5_BOVIN (DNA replication complex GINS protein SLD5 OS=Bos taurus OX=9913 GN=GINS4 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.108e-14
Identity = 61/209 (29.19%), Postives = 105/209 (50.24%), Query Frame = 2 
Query:   95 ESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYL-------KEAPNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            +S+E  +T  ELI +++  W NEK +  LLE + EI++ +M QL+  EEN    +K  L   I ++ +ERI+++++ Y R R+ KIE++  + L       +E P  L+P E + A  +      +L +  +    P  +  +L + V  P+ D +VF    +  E  + E  N       I +  G Q +  +R+V   V  G+I LI
Sbjct:   15 DSEEVILTPAELIDRLEQAWMNEKFAPELLENKSEIVECVMEQLEHMEENLKRAKKGDLKVSIHQMEMERIRFVLSSYLRCRLMKIEKFFPHTLEKEKTRREEEPPILSPEELVFAKEFLANTETYLKDTALKHMPPNLQKVDLLRTVPKPDLDAYVFLRVKERQENILVEPENDEQRDYVIDLEEGSQHLMRYRTVAPLVASGAIQLI 223
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Match: sp|Q9P7C8|SLD5_SCHPO (DNA replication complex GINS protein sld5 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sld5 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.293e-10
Identity = 51/214 (23.83%), Postives = 107/214 (50.00%), Query Frame = 2 
Query:   95 ESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQH----ILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEAPN---NLTPAEKMHATLYQKLISNHLIENVIFQTNPR------KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            E+D ED+  +         W NE+ +  LL + +EI+  ++ +++   E   +      A      +++  +ER+K+++  Y RTRI+KI+++   Y++  PN    L+  E+ +   +Q+++  H +++ + +   +      K  NLS  V SP+ D  VFC    +V  +V E+F  + +   I++  GD  +  +  +  +++ G + LI
Sbjct:   17 ENDYEDLCTQ---------WVNERMAPDLLPFAEEIVSRVLDRIEAQRETLQLAIGTSSATSYRSVLMQTELERVKFVLRSYMRTRINKIDKYAQ-YIQSHPNLLLYLSSPERQYLLRHQQIVHRHYMDSFLREVPAKMNKLDDKVGNLS-MVASPDMDTAVFC----VVNESVEENFRVSENEY-ITLDKGDVLILRYSVISDYLRLGVVSLI 214
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Match: A0A224YND5 (DNA replication complex GINS protein SLD5 OS=Rhipicephalus zambeziensis OX=60191 PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.198e-21
Identity = 68/220 (30.91%), Postives = 119/220 (54.09%), Query Frame = 2 
Query:   71 IMNEEILYESDEEDIT-CEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEAPNN-------LTPAEKMHATLYQKLISNHLIENVIFQTNP--RKDFNLSQFVNSPNSDKFVFC-FAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            I N+    ES EE+ T  E++++K++  WQNE  +  LL YQ +I+D ++ QL+  +EN     K      + K+ +ERI+YI+T Y RTR+ KIE+   Y L++           L+P E+  A  Y   I ++L  +V     P  ++DFN++     P+ D+FVF     + + + V+E  + + D   I +  G + +  +R++ + VK GS++L+
Sbjct:   10 IANKYGYVESHEEECTSAEQVVQKLEEAWQNETFAPELLPYQNDIVDCVLDQLRYMQENLQRIDKTDFKATMHKMEVERIQYILTSYLRTRLTKIEKHGAYILRQLETGELDSDELLSPYERKFARAYIASIEDYL-RDVALVHMPFNQQDFNMASVDRGPDLDEFVFLKVKRQTMGVLVDEGTSQSRDGEVIDLDEGSRHIMRYRAIASLVKDGSVVLL 228
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Match: A0A419QH65 (DNA replication complex GINS protein SLD5 OS=Clonorchis sinensis OX=79923 GN=Gins4 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 2.154e-21
Identity = 73/228 (32.02%), Postives = 127/228 (55.70%), Query Frame = 2 
Query:   80 EEILYESDEED------ITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQL-KIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-----KDFNLSQFVNSPNSDKFVFCFAMKLV-EINVNEDFNANSDMLTIS--VHAGDQFVAPFRSVETHVKKGSILLI 697
            EEI+  SDEE+      +T  EL+K++  +WQNEK +  LL    +++ L+  +  ++  +   +   +L AQ I ++ +ERI+Y+I+DY RTR+ K+E++ ++ L E        P +LT  E + A  Y   I  +L ++VI    P      KD +L+ F  SP+S  +VFC A+  V  + V+E     S   T+S  +  G+Q + P+ +++ HV+ G+++LI
Sbjct:   10 EEIIGGSDEENEADTELLTPAELLKRLVKVWQNEKVAPELLGAHPDLLSLVQDETDRLEAQAKSLPAGDLRAQ-IKRLQVERIRYMISDYMRTRVSKVEQFAEHILAEERARPDTEPPHLTAEEFLFAKSYTNSIKEYL-KSVIVSRLPANMQTVKDEDLA-FHPSPHS--YVFCRALSRVSSVVVDEPSGGESQSRTVSLTLEPGEQHLLPYSAIKQHVESGNVILI 232
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Match: A0A068WVG2 (Dna replication complex gins protein sld5 (Fragment) OS=Echinococcus granulosus OX=6210 GN=EgrG_001163250 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 7.990e-21
Identity = 64/209 (30.62%), Postives = 108/209 (51.67%), Query Frame = 2 
Query:  107 EDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEAPN-------NLTPAEKMHATLYQKLISNHLIENVIFQTNPRKDFNLSQ-----FVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            E +T  +LIK+   IWQNEK +  L+  Q + + L+  ++  +E       K  +   ++++  ER++Y++TDY RTRI KIE++  Y + E  +       +LTPAE      + K   +  IEN+ F        N+S+         PN D +VFC     +E+   +   A+S++LT+    G+  + P+R+V+ H K GS+ LI
Sbjct:   34 ESVTLPQLIKRFYKIWQNEKIAPELMPAQIDAVALISREVTNFETQAKSLPKGDIRAQLIRMQAERLRYLLTDYLRTRIRKIEQFPHYVISEERSRPQTEDPHLTPAE----FTFTKTFDDLTIENLRFTVLDHLPHNMSKVDQDAIAFRPNLDAYVFCQVHSRIEVTNTDPSGASSEVLTL--MPGEIHLLPYRAVQKHTKSGSVTLI 236
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Match: A0A564YUS5 (DNA replication complex GINS protein SLD5 OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS9774 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.374e-20
Identity = 66/209 (31.58%), Postives = 107/209 (51.20%), Query Frame = 2 
Query:  107 EDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYL--------KEAPNNLTPAEKMHATLYQKLISNHLIENVI--FQTNPRKDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDML--TISVHAGDQFVAPFRSVETHVKKGSILLI 697
            E +T  ELIK+ K IWQNEK S  L+  Q  II L++ +L  +E+  +   +  L   ++K+ +ER++Y +TDY R RI KIE++  Y +         E P +L+PAE   A ++     N L   V+     N RK  +       PN D +VFC     +EI  N      S++   T+++   +  + P+R +  H + GS++L+
Sbjct:   63 ESVTLPELIKRFKKIWQNEKLSPELMTAQTGIISLIVNELTEFEQQINSQPRGELKTQLMKLQVERLRYFLTDYIRVRIRKIEQYPHYVIMEERARSQAEEP-HLSPAEFNFAKIFDNATLNSLRSCVLEHLPVNMRK-LDPDAIALRPNLDSYVFCQINNRIEITENSVSQGASNLPTNTLTLMPREIHLLPYRVIRQHTEDGSVILL 269
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Match: A0A131Z4K9 (DNA replication complex GINS protein SLD5 OS=Rhipicephalus appendiculatus OX=34631 PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.594e-20
Identity = 67/220 (30.45%), Postives = 118/220 (53.64%), Query Frame = 2 
Query:   71 IMNEEILYESDEEDIT-CEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEAPNN-------LTPAEKMHATLYQKLISNHLIENVIFQTNP--RKDFNLSQFVNSPNSDKFVFC-FAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            I N+    ES EE+ T  E++++K++  W NE  +  LL YQ +I+D ++ QL+  +EN     K      + K+ +ERI+YI+T Y RTR+ KIE+   Y L++           L+P E+  A  Y   I ++L  +V     P  ++DFN++     P+ D+FVF     + + + V+E  + + D   I +  G + +  +R++ + VK GS++L+
Sbjct:   10 IANKYGYVESHEEECTSAEQVVQKLEEAWLNETFAPELLPYQNDIVDCVLDQLRYMQENLQRIDKTDFKATMHKMEVERIQYILTSYLRTRLTKIEKHGAYILRQLETGEIDSDELLSPYERKFARAYIASIEDYL-RDVALVHMPFNQQDFNIASVDGGPDLDEFVFLKVKRQTMGVLVDEGTSQSRDGEVIDLDEGSRHIMRYRAIASLVKDGSVVLL 228
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Cavefish
Match: gins4 (GINS complex subunit 4 [Source:NCBI gene;Acc:103046894])

HSP 1 Score: 73.1738 bits (178), Expect = 4.757e-15
Identity = 57/209 (27.27%), Postives = 102/209 (48.80%), Query Frame = 2 
Query:   95 ESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEA-------PNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            ES E+ +T  ELI +++  W NEK S  LLE + E+++ +M QL   EEN    +K  L   + ++ I+RI+++++ Y R+R+ KIE++  + L++        P+ L+P E   A  Y      +L    +    P  +  ++ + V  P  D FVF    +  E  + E          + +  G Q +  +R++   V  G++ LI
Sbjct:   17 ESQEDVLTPAELISRLEEAWLNEKFSPELLENKSELVECVMEQLTHMEENLQRVKKGDLKASVHRMEIDRIRFVLSSYLRSRLQKIEKFFPHVLEKEKSRAEGDPSFLSPEEFAFAKEYLANTEVYLKAVALKHMPPNLQSIDMLKAVPEPCLDSFVFLRVRERQENIMVEPETDEQREYVVDLEEGSQHLMRYRTIAPLVASGAVQLI 225
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Nematostella
Match: EDO37714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEX8])

HSP 1 Score: 79.7221 bits (195), Expect = 7.456e-18
Identity = 66/218 (30.28%), Postives = 118/218 (54.13%), Query Frame = 2 
Query:   95 ESDEE-DITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTD-INQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHA--------TLYQKLISNHLIENVIFQTNPRKDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            +SDEE +IT  E+++K++  W NEK S  LLE + ++++ ++ Q+K  E+N + + QK L+A    K+ I+RI+Y+++ Y RTR+ KIE+   + L++        P+ L+P E  +A        +L++ L+  H+  N+  Q+  RK     + V  PN D +VF    K++E    E    + +   I +  G Q +  + +V   +  GSI L+
Sbjct:   16 DSDEEIEITAAEVLEKLQEAWNNEKFSPELLESKSDLLECMLDQIKGMEKNLERVKQKNLVAT-FHKLEIDRIRYVLSSYLRTRLQKIEKHVVHILEQEASRDADEPSRLSPEELQYAKEYADNMESLFKSLVLQHMPPNM--QSIDRK-----KSVPRPNLDSYVF---FKVLED--QEQVMIDPEEQPIDLEKGAQHIMRYSAVAPLLANGSIALL 220
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Nematostella
Match: EDO26653 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TBF5])

HSP 1 Score: 47.7506 bits (112), Expect = 4.151e-7
Identity = 41/151 (27.15%), Postives = 74/151 (49.01%), Query Frame = 2 
Query:  290 IHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHA--------TLYQKLISNHLIENVIFQTNPRKDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            + I+RI+Y+++ Y RTR+ KIE+   + L++        P+ L+P E  +A        +L++ L+  H+  N+  Q+  RK     + V  PN D +VF    K++E    E    + +   I +  G Q +  + +V   +  GSI L+
Sbjct:    1 MQIDRIRYVLSSYLRTRLQKIEKHVVHILEQEASRDADEPSRLSPEELQYAKEYADNMESLFKSLVLQHMPPNM--QSIDRK-----KSVPRPNLDSYVF---FKVLED--QEQVMIDPEEQPIDLEKGAQHIMRYSAVAPLLANGSIALL 139
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Medaka
Match: gins4 (GINS complex subunit 4 [Source:NCBI gene;Acc:101172339])

HSP 1 Score: 67.3958 bits (163), Expect = 6.195e-13
Identity = 54/209 (25.84%), Postives = 100/209 (47.85%), Query Frame = 2 
Query:   95 ESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE-------APNNLTPAEKMHATLYQKLISNHLIENVIFQTNPR-KDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            +S E+ +T  ELI K++  W NEK S  L E + E+++ +M QL   E N    +K      I ++ ++RI++++T Y R+R+ KIE++  + L+         P+ L+P E   A  Y      +L    + +  P  +  ++ + V  P  D FVF    +  E  + E    +     + +  G Q +  +R++   +  G++ LI
Sbjct:   16 DSQEDVMTPAELIAKLEEAWLNEKFSPELQENKSEVVECVMEQLTHMESNLQRVKKGDTKASIHRMEVDRIRFVLTSYLRSRLQKIEKFFPHVLEREKSRADGEPSLLSPEEFAFAKEYYDNTEAYLKAVALKRMPPNLQSIDMLKAVPEPCLDSFVFLRVKEKQENILIEPETDDQREYVVDLEEGSQHLMRYRTIAPLISNGAVKLI 224
[Close]
BLAST of DNA replication complex GINS protein SLD5 vs. Planmine SMEST
Match: SMESG000062770.1 (SMESG000062770.1)

HSP 1 Score: 410.609 bits (1054), Expect = 5.733e-147
Identity = 202/208 (97.12%), Postives = 203/208 (97.60%), Query Frame = 2 
Query:   74 MNEEILYESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEAPNNLTPAEKMHATLYQKLISNHLIENVIFQTNPRKDFNLSQFVNSPNSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHVKKGSILLI 697
            MNEEILYESDEEDITCEELIKKMKLIWQNEKASVILLEYQ EIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKE PNNLTPAEK HATLYQKLISNHLIENVI QTNPRKDFNLSQFVNSPNSDKFVFCFAMK VEINVNEDFNANSD+LTISVHAGDQFVAPFRSVETHVKKGSILLI
Sbjct:    1 MNEEILYESDEEDITCEELIKKMKLIWQNEKASVILLEYQTEIIDLLMTQLKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYYLKEDPNNLTPAEKTHATLYQKLISNHLIENVISQTNPRKDFNLSQFVNSPNSDKFVFCFAMKSVEINVNEDFNANSDILTISVHAGDQFVAPFRSVETHVKKGSILLI 208
The following BLAST results are available for this feature:
BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GINS42.030e-1729.52GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGN... [more]
GINS42.030e-1729.52GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGN... [more]
GINS41.366e-1431.65GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGN... [more]
GINS42.073e-1037.65GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGN... [more]
GINS43.648e-1038.10GINS complex subunit 4 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sld-52.623e-1035.16DNA replication complex GINS protein SLD5 [Source... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sld54.779e-724.29gene:FBgn0039403 transcript:FBtr0084975[more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gins46.628e-1426.54GINS complex subunit 4 (Sld5 homolog) [Source:ZFIN... [more]
gins46.183e-1326.11GINS complex subunit 4 (Sld5 homolog) [Source:ZFIN... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gins48.367e-1530.05GINS complex subunit 4 (Sld5 homolog) [Source:NCBI... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gins41.039e-1730.00GINS complex subunit 4 (Sld5 homolog) [Source:MGI ... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q99LZ3|SLD5_MOUSE7.266e-1730.00DNA replication complex GINS protein SLD5 OS=Mus m... [more]
sp|Q9BRT9|SLD5_HUMAN9.748e-1729.52DNA replication complex GINS protein SLD5 OS=Homo ... [more]
sp|Q499W2|SLD5_RAT1.432e-1629.52DNA replication complex GINS protein SLD5 OS=Rattu... [more]
sp|A2VE40|SLD5_BOVIN1.108e-1429.19DNA replication complex GINS protein SLD5 OS=Bos t... [more]
sp|Q9P7C8|SLD5_SCHPO4.293e-1023.83DNA replication complex GINS protein sld5 OS=Schiz... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A224YND51.198e-2130.91DNA replication complex GINS protein SLD5 OS=Rhipi... [more]
A0A419QH652.154e-2132.02DNA replication complex GINS protein SLD5 OS=Clono... [more]
A0A068WVG27.990e-2130.62Dna replication complex gins protein sld5 (Fragmen... [more]
A0A564YUS51.374e-2031.58DNA replication complex GINS protein SLD5 OS=Hymen... [more]
A0A131Z4K91.594e-2030.45DNA replication complex GINS protein SLD5 OS=Rhipi... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gins44.757e-1527.27GINS complex subunit 4 [Source:NCBI gene;Acc:10304... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO377147.456e-1830.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO266534.151e-727.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gins46.195e-1325.84GINS complex subunit 4 [Source:NCBI gene;Acc:10117... [more]
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BLAST of DNA replication complex GINS protein SLD5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000062770.15.733e-14797.12SMESG000062770.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033400 ID=SMED30033400|Name=DNA replication complex GINS protein SLD5|organism=Schmidtea mediterranea sexual|type=transcript|length=853bp
AAAATTTACAAAGGTTTTCAAAATTAATAGTTTTATAATTGAAAAGAATT
TTATACAATAGATTTCATTTATTATGAATGAGGAAATTTTATATGAATCA
GACGAGGAAGACATTACTTGTGAAGAATTAATTAAAAAAATGAAATTAAT
TTGGCAGAATGAAAAAGCTTCGGTTATTTTACTTGAATATCAAAAAGAAA
TTATTGATCTGCTAATGACACAGTTGAAAATCTATGAGGAAAATACAGAT
ATCAACCAGAAAGAATTACTCGCTCAGCACATTCTAAAAATTCATATTGA
AAGAATAAAGTATATAATTACTGATTATTCAAGAACAAGAATTCATAAGA
TTGAAGAATGGTGCGATTATTACCTCAAAGAAGCCCCAAATAATTTAACA
CCAGCTGAAAAAATGCATGCTACACTGTATCAGAAATTAATCTCGAATCA
TTTAATAGAAAACGTTATTTTTCAAACGAATCCTAGAAAAGATTTTAATT
TATCTCAATTTGTTAATTCTCCGAATTCGGATAAATTTGTTTTCTGTTTT
GCTATGAAATTGGTGGAAATCAATGTTAATGAAGATTTCAATGCAAATTC
TGATATGTTGACAATCAGTGTGCATGCTGGCGATCAGTTCGTTGCTCCAT
TTCGGTCGGTTGAAACCCACGTGAAAAAAGGCTCCATATTGTTAATATAA
ATTTATTTTTATCTCCGCCGTGCAAATTATGATGCGCATCATTGGATTCG
GTAAATGTAAAAACGCGTATGAGTTATCGCGCATTTCGAAATGTTTTGTC
CAACGGTCGTTTTGAGTTTTATTATTTTTTTTATACTTAAAAATGAGTTC
GGG
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protein sequence of SMED30033400-orf-1

>SMED30033400-orf-1 ID=SMED30033400-orf-1|Name=SMED30033400-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp
MNEEILYESDEEDITCEELIKKMKLIWQNEKASVILLEYQKEIIDLLMTQ
LKIYEENTDINQKELLAQHILKIHIERIKYIITDYSRTRIHKIEEWCDYY
LKEAPNNLTPAEKMHATLYQKLISNHLIENVIFQTNPRKDFNLSQFVNSP
NSDKFVFCFAMKLVEINVNEDFNANSDMLTISVHAGDQFVAPFRSVETHV
KKGSILLI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006261DNA-dependent DNA replication
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR038749Sld5_GINS_A
IPR036224GINS_bundle-like_dom_sf
IPR031633SLD5_C
IPR008591GINS_Sld5
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.58.1030coord: 7..198
e-value: 7.1E-38
score: 132.4
NoneNo IPR availableSUPERFAMILYSSF160059PriA/YqbF domaincoord: 151..208
IPR031633DNA replication complex GINS protein SLD5, C-terminalPFAMPF16922SLD5_Ccoord: 150..208
e-value: 8.5E-9
score: 35.5
IPR008591GINS complex subunit Sld5PIRSFPIRSF007764GINS_Sld5coord: 1..208
e-value: 2.6E-31
score: 106.7
IPR008591GINS complex subunit Sld5PANTHERPTHR21206SLD5 PROTEINcoord: 16..208
IPR038749GINS complex protein Sld5, alpha-helical domainCDDcd11711GINS_A_Sld5coord: 19..127
e-value: 5.16877E-27
score: 97.681
IPR036224GINS, helical bundle-like domain superfamilySUPERFAMILYSSF158573GINS helical bundle-likecoord: 14..137