SMED30033387
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033387 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12Note: Hover over icons to view figure legend
Alignments
SMED30033387 aligns in the following genomic locations:
Homology
BLAST of SMED30033387 vs. Planmine SMEST
Match: SMESG000017013.1 (SMESG000017013.1) HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0 Identity = 365/366 (99.73%), Postives = 366/366 (100.00%), Query Frame = 3 Query: 195 MNCVAITNSDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPXXXXXXXXXXXXHFAISTVSSVVEVMPANYLVCVPANYVIDKTDEFLDRFLPXXXXXXXXXXXXXXXXXRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNLLSEVQNKRMELKGSIMHEYFHAIETIKDSIPMIPLEHWNLMKEILYNXXXXXXXXXXXXXXXXXFMVKNALEISISXXXXXXXXXXXXXKANPEFVSDSTLAYSTNVITLTRAWLEFFINFFKLIHLIGVRPLMAIM 1292 MNCVAITNSDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPVTKTIKTVSKTSHFAISTVSSVVEVMPANYLVCVPANYVIDKTDEFLDRFLPEYDESSEEELENSNLYYRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNL+SEVQNKRMELKGSIMHEYFHAIETIKDSIPMIPLEHWNLMKEILYNSKELLKSLSISSTLTIEFMVKNALEISISLNKNLLNLQNMLNKANPEFVSDSTLAYSTNVITLTRAWLEFFINFFKLIHLIGVRPLMAIM Sbjct: 1 MNCVAITNSDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPVTKTIKTVSKTSHFAISTVSSVVEVMPANYLVCVPANYVIDKTDEFLDRFLPEYDESSEEELENSNLYYRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNLVSEVQNKRMELKGSIMHEYFHAIETIKDSIPMIPLEHWNLMKEILYNSKELLKSLSISSTLTIEFMVKNALEISISLNKNLLNLQNMLNKANPEFVSDSTLAYSTNVITLTRAWLEFFINFFKLIHLIGVRPLMAIM 366
BLAST of SMED30033387 vs. Planmine SMEST
Match: SMESG000017016.1 (SMESG000017016.1) HSP 1 Score: 236.884 bits (603), Expect = 1.265e-73 Identity = 154/353 (43.63%), Postives = 238/353 (67.42%), Query Frame = 3 Query: 195 MNCVAITNSDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPXXXXXXXXXXXXHFAISTVSSVVE-----VMPANYLVCVPANYVIDKTDEFLDRFLPXXXXXXXXXXXXXXXXXRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNLLSEVQNKRMELKGSIMHEYFHAIETIKDSIPMIPLEHWNLMKEILYNXXXXXXXXXXXXXXXXXFMVKNALEISISXXXXXXXXXXXXXKANPEFVSDSTLAYSTNVITLTRAWLEFFI 1238 M C +++ + +KS+D++Q YPIVNDS+ +V+ YY+W+KS+N II+N LTKAE T+K VK++ EPIA L +ID+V+ NQLE+LEK CPIIL+SS +I + SK+Y + ES KT+ WPV KT+ TVS+TSH AISTVSSVV V+P+ L V NY++DKTDE LD++ P+ ++ +EE + N ++ +++R S +TQ R+ R+A +I+LL K+S EKL +IQ ++ E+ ++ + ++ EY H ++ IK + P+ + H L++E L SK L K +S S+ +T+E+MV +AL +N NL LQN L ++ PE ++ L +++ +T R L+ FI Sbjct: 1 MAC-SVSQNCSHFKSLDRVQNYPIVNDSIGFVLSYYQWIKSSNGIIDNTLTKAEMTLKAVKDTAEPIAVRNLGFIDNVVVNQLEKLEKKCPIILESSSSIKNIPSKIYHDGIESTKKTLMWPVCKTLDTVSRTSHMAISTVSSVVATSMDIVIPSTRLTNVSTNYLLDKTDELLDKYFPDNEQCAEEPISNPDIMTKIKRLSGKTQKRLYRRASERINLLLKASYEKLNQIQMMVEEINKQKETFQSAVAKEYSHTLDVIKANNPLNIVNHSQLVQEALNYSKSLKKVVSESTDVTLEYMVNSALGGLDVINNNLFKLQNFLQQSRPENIAGEILIHTSTAVTEIRIVLDAFI 352
BLAST of SMED30033387 vs. Planmine SMEST
Match: SMESG000016757.1 (SMESG000016757.1) HSP 1 Score: 87.0409 bits (214), Expect = 1.529e-18 Identity = 63/247 (25.51%), Postives = 119/247 (48.18%), Query Frame = 3 Query: 219 SDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPXXXXXXXXXXXXHFAISTVSSVVEVMPANYLVCVPANYVIDKTDEFLDRFLPXXXXXXXXXXXXXXXXXRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNLLSEVQNKRMELKGSIMHEYFHAIETI 959 +D ++S D++ YPIV++S+ + Y+W+KS N I TK E + +++ ST+PIAA+KLK +D V+ QLE+ EK P++L + + + + + N +++ + H VS++ + +Y V + + + F+PE D++ S+ + +FS +T R+ + + +ID QK ++E L+ I L + +Q + L I +E I Sbjct: 3 NDIIFQSFDRICHYPIVHESVGCFLKVYQWIKSRNKTIHGTFTKVETVLISIQNSTKPIAASKLKPLDQVVNKQLEKFEKFFPVVLDDTDVVLKNTIRSFVNPITNSISCV--------------VHKGFKLVSNLSSLTKLDY-----EELVYNTIENMFNYFIPEIDDTWHVSSNCSHKNQKSMKFSKKTFHRLSKIKIIEIDRFQKLNVEYLQNIHILANRIQKESGGLYNEIPSNIVQLMEDI 230
BLAST of SMED30033387 vs. Planmine SMEST
Match: SMESG000016757.1 (SMESG000016757.1) HSP 1 Score: 86.6557 bits (213), Expect = 1.812e-18 Identity = 63/247 (25.51%), Postives = 119/247 (48.18%), Query Frame = 3 Query: 219 SDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTIFWPXXXXXXXXXXXXHFAISTVSSVVEVMPANYLVCVPANYVIDKTDEFLDRFLPXXXXXXXXXXXXXXXXXRVRRFSARTQTRICRQALSKIDLLQKSSLEKLERIQNLLSEVQNKRMELKGSIMHEYFHAIETI 959 +D ++S D++ YPIV++S+ + Y+W+KS N I TK E + +++ ST+PIAA+KLK +D V+ QLE+ EK P++L + + + + + N +++ + H VS++ + +Y V + + + F+PE D++ S+ + +FS +T R+ + + +ID QK ++E L+ I L + +Q + L I +E I Sbjct: 3 NDIIFQSFDRICHYPIVHESVGCFLKVYQWIKSRNKTIHGTFTKVETVLISIQNSTKPIAASKLKPLDQVVNKQLEKFEKFFPVVLDDTDVVLKNTIRSFVNPITNSISCV--------------VHKGFKLVSNLSSLTKLDY-----EELVYNTIENMFNYFIPEIDDTWHVSSNCSHKNQKSMKFSKKTFHRLSKIKIIEIDRFQKLNVEYLQNIHILANRIQKESGGLYNEIPSNIVQLMEDI 230
BLAST of SMED30033387 vs. Planmine SMEST
Match: SMESG000075481.1 (SMESG000075481.1) HSP 1 Score: 82.4185 bits (202), Expect = 5.271e-17 Identity = 41/111 (36.94%), Postives = 67/111 (60.36%), Query Frame = 3 Query: 219 SDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALTKAEKTIKTVKESTEPIAANKLKYIDHVLYNQLERLEKTCPIILQSSKTIADASSKVYRNNFESAVKTI 551 SD KS++++++YP++ D+ YY W+K NS + + LT E +K VK+ EPIAA+KL +D+ NQL++LE PII +SS+ + +S + Y + +V I Sbjct: 6 SDSQIKSLERIREYPVIKDTFTKAGEYYSWIKDKNSKLNSTLTWTESNLKIVKDMVEPIAASKLGCVDNFACNQLDKLENKYPIIKESSENVIKSSGEYYETSIRPSVDRI 116 The following BLAST results are available for this feature:
BLAST of SMED30033387 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30033387 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30033387 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30033387 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30033387 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033387 ID=SMED30033387|Name=SMED30033387|organism=Schmidtea mediterranea sexual|type=transcript|length=1371bpback to top protein sequence of SMED30033387-orf-1 >SMED30033387-orf-1 ID=SMED30033387-orf-1|Name=SMED30033387-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=367bp MNCVAITNSDPTYKSIDKMQQYPIVNDSLVYVIGYYKWLKSTNSIIENALback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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