SMED30033381

Overview
NameSMED30033381
Smed IDSMED30033381
Length (bp)1568
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033381 (SMED30033381) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033381 (SMED30033381) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033381 (SMED30033381) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033381 (SMED30033381) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30033381

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30033381SMESG000022853.1 SmedASXL_007704SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
neuronSMED30033381SMESG000022853.1 dd_Smed_v4_12892_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30033381SMESG000022853.1 dd_Smed_v6_12892_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033381 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001401:84034..88210 +7792
Homology
BLAST of SMED30033381 vs. Ensembl Human
Match: SAXO1 (stabilizer of axonemal microtubules 1 [Source:HGNC Symbol;Acc:HGNC:28566])

HSP 1 Score: 53.5286 bits (127), Expect = 6.135e-7
Identity = 73/303 (24.09%), Postives = 119/303 (39.27%), Query Frame = 2
Query:  323 TFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQ--PSEK-MAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTS----NLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLP 1210
               S Y E+Y    S      FKP  + +      +GLT    +F   K+  VK    + QY Q  PSE+ M   + YK D+      R    KP+DS    SD  E + T Y  D+   +  + +  R    Y+P      + T +Q ++  +    T + KP    ++   P    TNY   +          +  + F+P       +IPF SL+  ++ ++    +P K  S KP +     D PF  T+ +   +Q    +P      K    Y  P + +   ++ QA +   K  P
Sbjct:   32 CLLSEYTENYPFYHSYLPRESFKPRREYQKGPIPMEGLTTSRRDFGPHKVAPVK----VHQYDQFVPSEENMDLLTTYKKDYNPYPVCRVDPIKPRDSKYPCSDKMECLPT-YKADYLPWNQPRREPLRLEHKYQPASVRFDNRTTHQDDYPIKGLVKTISCKPLAMPKLCNIPLEDVTNYKMSYVAHPVEKRFVHEAEKFRPC------EIPFESLTTQKQSYRGLMGEPAK--SLKPLARPPGLDMPFCNTTEFRDKYQA---WPMPRMFSKAPITYVPPEDRMDLLTTVQAHYTCPKGAP 318          
BLAST of SMED30033381 vs. Ensembl Zebrafish
Match: saxo2 (stabilizer of axonemal microtubules 2 [Source:ZFIN;Acc:ZDB-GENE-051127-27])

HSP 1 Score: 51.6026 bits (122), Expect = 1.697e-6
Identity = 60/299 (20.07%), Postives = 130/299 (43.48%), Query Frame = 2
Query:  326 FKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTS--NLTKNFKPKNPFHSPDIPFNSLSNY-QEGFQ-VTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLP 1210
              + Y++D+     ++  +P +P  ++ +     D +  Y E+F   +I++ +  KP   YQ P+ K    + ++ DF  +     +  KP +   LS    E  ++N  + +     E  +  +P + YKP+      +T ++ ++   P   T++ KP+       +PF S+T +   + ++  S   + K+ +  +P  + D+   S S+Y +   Q     KP  LP+         S  PF   +T   DFQ      ++    +  +    P+  +   ++++A F+ +++ P
Sbjct:  103 LGTTYKQDFN-PYEVQPFTPLRPKERVHVVNAKLDTVPTYKEDFRQWEIIRRELTKPDTTYQPPATKFGNSTTFQDDFVHRGLVPRESYKPSNVAKLSDTPFEKNTSNKLS-YVPHPLE-ARYVKPPEEYKPSSHPFHDVTTHRQDYQGLPSQSTKSCKPEPVKVASNKPFQSSTEFRDQYQHWPVSLPQMQKSVEYTSPTANMDLTTTSHSDYIKHQIQPFVSSKPFSLPA--------KSSRPFQGNTTMRDDFQPWVA--QRQSIIRKQAELQRPSGKMEDLTTFRAHFVQHEAQP 388          
BLAST of SMED30033381 vs. Ensembl Mouse
Match: Saxo1 (stabilizer of axonemal microtubules 1 [Source:MGI Symbol;Acc:MGI:1923061])

HSP 1 Score: 53.1434 bits (126), Expect = 5.885e-7
Identity = 70/260 (26.92%), Postives = 107/260 (41.15%), Query Frame = 2
Query:  332 SAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKP----KISYEIPTEPFHSNTNYSSDFTNFKTS----NLTKNF-KPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQ 1084
            S Y E+Y I +S    + FKP           +GLT    +F   K++ V   +P   Y    E M   + YK DF         + KP+DS   + D        Y  D+   +  + +  RP   Y+P      + T +Q ++  +    T + KP    KI Y IP E          D TN+K S     + K F K    +   DIPF +L+ ++E ++    +P K  S KP +++   D PF  T+     FQ
Sbjct:   36 SEYTENYPIYQSYLPRNSFKPEWCYRKPSAPMEGLTTCRRDFGLHKMLPVTFHQPDT-YVPSQETMDLLTTYKHDFNYLPTCPVGLIKPRDSKFPNGDKIMQYLPTYKVDYLPWNQPRRELLRPPHKYRPESSKFENRTTHQDDYTMKGLVTTVSCKPPSKSKI-YNIPLE----------DLTNYKMSYVPHPVEKRFVKEAEKYIPCDIPFENLTTHKESYRGLLGEPAK--STKPPAKFPVHDAPFTNTTEVQEKFQ 281          

HSP 2 Score: 51.2174 bits (121), Expect = 2.354e-6
Identity = 72/298 (24.16%), Postives = 122/298 (40.94%), Query Frame = 2
Query:  413 SKENFDGLTNYNEEFVNKKIVKVKSLKPI-IQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRP---NDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTE-PFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQ-------VTKEKPIKLP------------------------SFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIAN 1198
            S+E  D LT Y  +F       V  +KP   ++    + M +   YK D+   N  R ++ +P   +   S   EN  T +  D++ K      S +P   + IY    +D   LTNY+M ++  P    + F  +    IP + PF + T +   +         K+ KP   F   D PF + +  QE FQ       V K   + +P                        + +P    K S + F++++T   DF++ +     T+  +PT + S P EPL  Q++ +  F+A+
Sbjct:   96 SQETMDLLTTYKHDFNYLPTCPVGLIKPRDSKFPNGDKIMQYLPTYKVDYLPWNQPRRELLRPPHKYRPESSKFEN-RTTHQDDYTMKGLVTTVSCKPPSKSKIYNIPLED---LTNYKMSYVPHPVE--KRFVKEAEKYIPCDIPFENLTTHKESYRGL-LGEPAKSTKPPAKFPVHDAPFTNTTEVQEKFQAWPTPQMVPKAPVVYVPPEEKMDLLTTVQTHYKYLKGSPAITCRPVPSIKKS-KCFESSTTTKDDFKQWAAV--NTKPIRPTLHLSLPAEPLDCQTTTKTCFVAH 383          
BLAST of SMED30033381 vs. UniProt/SwissProt
Match: sp|Q8IYX7|SAXO1_HUMAN (Stabilizer of axonemal microtubules 1 OS=Homo sapiens OX=9606 GN=SAXO1 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 2.946e-6
Identity = 73/303 (24.09%), Postives = 119/303 (39.27%), Query Frame = 2
Query:  323 TFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQ--PSEK-MAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTS----NLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLP 1210
               S Y E+Y    S      FKP  + +      +GLT    +F   K+  VK    + QY Q  PSE+ M   + YK D+      R    KP+DS    SD  E + T Y  D+   +  + +  R    Y+P      + T +Q ++  +    T + KP    ++   P    TNY   +          +  + F+P       +IPF SL+  ++ ++    +P K  S KP +     D PF  T+ +   +Q    +P      K    Y  P + +   ++ QA +   K  P
Sbjct:   32 CLLSEYTENYPFYHSYLPRESFKPRREYQKGPIPMEGLTTSRRDFGPHKVAPVK----VHQYDQFVPSEENMDLLTTYKKDYNPYPVCRVDPIKPRDSKYPCSDKMECLPT-YKADYLPWNQPRREPLRLEHKYQPASVRFDNRTTHQDDYPIKGLVKTISCKPLAMPKLCNIPLEDVTNYKMSYVAHPVEKRFVHEAEKFRPC------EIPFESLTTQKQSYRGLMGEPAK--SLKPLARPPGLDMPFCNTTEFRDKYQA---WPMPRMFSKAPITYVPPEDRMDLLTTVQAHYTCPKGAP 318          
BLAST of SMED30033381 vs. UniProt/SwissProt
Match: sp|Q4R7D3|SAXO1_MACFA (Stabilizer of axonemal microtubules 1 OS=Macaca fascicularis OX=9541 GN=SAXO1 PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 3.300e-6
Identity = 73/303 (24.09%), Postives = 121/303 (39.93%), Query Frame = 2
Query:  323 TFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQ--PSEK-MAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTS----NLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLP 1210
               S Y E+Y    S      FKP  + +      +GLT    +F   K+  VK+     QY Q  PSE+ M   + YK D+      R    KP+DS    S+  E + T Y  D+   +  + Q  R    Y+P      + T +Q ++  +    T + KP    ++   P    TNY   +          +  + F+P       +IPF SL+ +++ ++    +P K  S KP +     D PF  T+ +   +Q    +P      K    Y  P + +   ++ QA +   K +P
Sbjct:   32 CLLSEYTENYPCYHSYLPRESFKPRREYQKGSIPMEGLTTSRRDFGPHKVAPVKAH----QYDQFVPSEENMDLLTTYKKDYNPYTVCRVDPIKPRDSKYPYSNKMEYLPT-YKADYLPWNQPRRQPLRLEHKYQPASVRFDNRTTHQDDYPIKGLVKTVSCKPLAMPKLCNIPLEDVTNYKMSYVAHPVEKRFVHEAEKFRPC------EIPFESLTTHKQSYRGLMGEPAK--SLKPLARPPGLDMPFSNTTEFRDKYQA---WPTPQMFSKAPITYVPPEDSMDLLTTVQAHYTYPKGVP 318          
BLAST of SMED30033381 vs. TrEMBL
Match: A0A183WTP7 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS15500 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 8.589e-16
Identity = 98/356 (27.53%), Postives = 152/356 (42.70%), Query Frame = 2
Query:  242 ISPRSYSKLPSIKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTN-------YTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNY---------QEGFQVTKEKPIKLP-----------------------SFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            I PRS  +LP      N  I+ NS   T++S Y+        +E    +KP       +E  D  T+Y  ++  +     +S KPI  Y  P   M   ++Y++++      +N    P++       DNE   T        Y  D+  K  E +Q FRP   Y+  ++     T YQME+   P S   ++ P   Y  P +PFH NT+YS +F  + T+  T++FKP   +  P       +NY         Q+G+    +  + LP                       + KPK     SDE   T ++Y+ DF+ L+  P  + S+KP   Y SP+ PL T SSY  D++
Sbjct:  124 IMPRSQMELP------NDQILLNS---TYESNYQ-----PYKLEPTINYKPYELYSPPQEPMDTQTSYRIDYPPRCPQTGRSYKPIEIYVPPLVPMLNKTSYQTEY------QNPPPSPRE-IPFRMKDNETFPTGRFQSETTYIKDYQTKRCEPVQCFRPYREYQVPKEPMSDKTCYQMEYTPHPLSKPNSYAPNYVYHQPEQPFHKNTSYSEEFQPY-TAPKTESFKPVFEYRKPKTKIEDTTNYRSDYRQWCTQKGYPYYPQDNLSLPQGKSNMTTSYMQEFQPRYSQRVQAIKPKENKWRSDEALSTQTSYLTDFRPLT--PHPSMSYKPIEIYKSPSLPLVTHSSYTEDYV 455          

HSP 2 Score: 68.5514 bits (166), Expect = 2.145e-8
Identity = 70/292 (23.97%), Postives = 131/292 (44.86%), Query Frame = 2
Query:  329 KSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPF--HS--------------------------------NTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKF--SDEPFDTTSTYVYDFQKLSN 1096
            K+ Y+ +YT    + K + + P       ++ F   T+Y+EEF      K +S KP+ +Y++P  K+   +NY+SD+      +     PQD+  L    + N++T+Y  +F  +  +++Q+ +P +    +++   + T+Y  +F      P+ ++KP   Y+ P+ P   HS                                NT+YS +F  ++ +   +N KP   +  P IPF+  ++YQ  F     K     S  P + Y +       DTT++Y +DF+ + N
Sbjct:  277 KTCYQMEYT-PHPLSKPNSYAPNYVYHQPEQPFHKNTSYSEEFQPYTAPKTESFKPVFEYRKPKTKIEDTTNYRSDYRQWCTQKGYPYYPQDNLSLPQGKS-NMTTSYMQEFQPRYSQRVQAIKPKENKWRSDEALSTQTSYLTDFRPLTPHPSMSYKPIEIYKSPSLPLVTHSSYTEDYVPRCAKKREPVLPIPNLKTSKLPMEKNTSYSEEF-QYRKAKKPENLKPAYQYTHPKIPFSGKTSYQIEFPPPPRKHKHNQSCPPYTRYSYIHPTTKMDTTTSYTHDFKSIRN 565          
BLAST of SMED30033381 vs. TrEMBL
Match: A0A183QDV9 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.242e-15
Identity = 95/372 (25.54%), Postives = 159/372 (42.74%), Query Frame = 2
Query:  275 IKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVS----------------------------------TNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQ---VTKEKPI-----------------------------KLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            ++  DN N ++    D F ++Y EDY       +  P  PL+Q E   +N    + Y +++   K+ KV   +P   Y  P E M  ++ Y  D+  +     K  KP +S+V  S    N++                                  T+Y TD+  K  + + SF+P   Y+   +  +S T+YQ++++ +P+S   ++ P   Y+ P  PF  NT+YS +F     S   ++FKP   +  P +   +++ Y   ++   V K+ P                              KL   K K     SDE   + +TY+ DF+ ++  PE + S+KPT  Y SP+ P+ +QSSY  DFI
Sbjct:  116 VENYDNLNRMA---ADPFLTSYTEDYKPYGKFYREDPIIPLSQWESPHDNDLLDSTYQDDYQPYKLDKVNDYRPREFYLPPQEPMDTNTTYNLDYPPRYSQMEKSFKPYESYVPPSVPMSNITSYQLEYEDPPWSPKEMPFRMKDHETFPKGKFQSETSYITDYPPKVAQPVHSFKPYRKYRTPTEPMLSQTSYQIDYIFQPRSKPTSYSPVYVYQQPKVPFQKNTSYSEEFQPHLFSK-PESFKPVYEYRKPRMKMENITAYSSDYRPWHVKKDDPFYPKDNIYLPRVNNEMITSYMDEFPSRYTQKLYPIKQKENRWLSDEALSSQTTYLNDFRPVT--PEPSMSYKPTEVYRSPSLPVVSQSSYAEDFI 481          
BLAST of SMED30033381 vs. TrEMBL
Match: A0A3S5B7S9 (Uncharacterized protein (Fragment) OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS36042 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.484e-15
Identity = 81/288 (28.12%), Postives = 139/288 (48.26%), Query Frame = 2
Query:  329 KSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            +++Y E++ I+R  EK    KP +Q E+  ++F   T Y  ++V ++    ++ KPI  Y  P   M   + Y  +F  K  ++ +  KP + ++   +     +T Y+ +F     E  Q+FRPN  + P E     +T+Y+ E+  + Q    + +P   Y  P   F   TNY++DF   K   LT+NFKPK+ +  P IP    + Y   F    ++  K  S +P + YK   + F   ++Y  DF  +    E T +FKP  +YS P  P+   + Y  +++
Sbjct:  186 RTSYNEEF-IRRIHEKQPSLKPKHQYEIPTKDFSKDTVYTTDYVPRRGEVSENYKPIRIYSPPEFSMEQQTTYSKEFPFKFQEKQRSTKPNNKYIPPLEKFAR-NTVYSVEFLPFTVEPTQNFRPNRTFSPPELPMDKITSYRNEYTPKFQHKQHSMQPSNKYVPPQLKFSKETNYNADFIPRKL-ELTENFKPKSIYSLPLIPMEQQTCYSGEFTPRVQE--KQESLRPANTYKPPVDEFAKDTSYNLDF--IPRVGEVTENFKPRFSYSLPETPMQKDTIYTIEYL 466          

HSP 2 Score: 82.4185 bits (202), Expect = 6.931e-13
Identity = 81/305 (26.56%), Postives = 144/305 (47.21%), Query Frame = 2
Query:  332 SAYREDYTIKRS--MEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQ 1240
            + Y E+Y  K +   +KV   KP    +  +E F   T Y+ EFV + +   ++ KP+  Y  P   M   ++Y  +FT     + +  KP++ +  S  +    +T Y +++     E+ QSF+P   Y P        T+Y  EF++R      + KPK  YEIPT+ F  +T Y++D+   +   +++N+KP   +  P+      + Y + F    ++  K  S KP ++Y    E F   + Y  +F   +   E T++F+P   +S P  P+   +SY+     N+  P+  HK+ + Q
Sbjct:   51 TTYSEEYIPKNAGLQKKV---KPSFTYQTPEERFSTQTVYSTEFVKRNLEHQQNFKPVRNYVVPDVGMEQETSYSKEFTPMPLQKQQSAKPKNHYHPSGGEFAK-TTAYKSEYIYNGGEQSQSFKPKRTYSPPALPMEQRTSYNEEFIRRIHEKQPSLKPKHQYEIPTKDFSKDTVYTTDYVP-RRGEVSENYKPIRIYSPPEFSMEQQTTYSKEFPFKFQE--KQRSTKPNNKYIPPLEKFARNTVYSVEFLPFTV--EPTQNFRPNRTFSPPELPMDKITSYR-----NEYTPKFQHKQHSMQ 341          

HSP 3 Score: 78.5666 bits (192), Expect = 1.155e-11
Identity = 70/251 (27.89%), Postives = 116/251 (46.22%), Query Frame = 2
Query:  437 TNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADF 1189
            T+YN+EFV K      S KP + +  P   M   + Y  ++  KN    K  KP  ++  + ++  +  T Y+T+F K++ E  Q+F+P   Y   +      T+Y  EF   P    Q+ KPK  Y      F   T Y S++  +     +++FKPK  +  P +P    ++Y E F   +    K PS KPK +Y+   + F   + Y  D+  +    E + ++KP   YS P   +  Q++Y  +F
Sbjct:   17 TSYNKEFVYKHAEPAASCKPELVFAPPIISMEKSTTYSEEYIPKNAGLQKKVKPSFTY-QTPEERFSTQTVYSTEFVKRNLEHQQNFKPVRNYVVPDVGMEQETSYSKEFTPMPLQKQQSAKPKNHYHPSGGEFAKTTAYKSEYI-YNGGEQSQSFKPKRTYSPPALPMEQRTSYNEEF--IRRIHEKQPSLKPKHQYEIPTKDFSKDTVYTTDY--VPRRGEVSENYKPIRIYSPPEFSMEQQTTYSKEF 261          

HSP 4 Score: 75.0998 bits (183), Expect = 1.626e-10
Identity = 56/183 (30.60%), Postives = 94/183 (51.37%), Query Frame = 2
Query:  425 FDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGF 973
             D +T+Y  E+  K   K  S++P  +Y  P  K +  +NY +DF  +  +  +  KP+  + L     E   T Y+ +F+ +  EK +S RP + YKP   +    T+Y ++F+ R    T+NFKP+ SY +P  P   +T Y+ ++   KT +  +N KPK  F  P   F   ++Y++ F
Sbjct:  319 MDKITSYRNEYTPKFQHKQHSMQPSNKYVPPQLKFSKETNYNADFIPRKLELTENFKPKSIYSLPLIPMEQ-QTCYSGEFTPRVQEKQESLRPANTYKPPVDEFAKDTSYNLDFIPRVGEVTENFKPRFSYSLPETPMQKDTIYTIEYLP-KTQSKQENMKPKFHFEPPLSEFTKDTSYRKEF 499          

HSP 5 Score: 66.2402 bits (160), Expect = 8.962e-8
Identity = 58/197 (29.44%), Postives = 93/197 (47.21%), Query Frame = 2
Query:  638 VSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKK 1228
            + T+Y  +F  K  E   S +P  ++ P        T Y  E++ +     +  KP  +Y+ P E F + T YS++F   +     +NFKP   +  PD+     ++Y + F  T     K  S KPK+ Y  S   F  T+ Y  ++  + N  E+++SFKP   YS P  P+  ++SY  +FI      R IH+K
Sbjct:   15 MDTSYNKEFVYKHAEPAASCKPELVFAPPIISMEKSTTYSEEYIPKNAGLQKKVKPSFTYQTPEERFSTQTVYSTEFVK-RNLEHQQNFKPVRNYVVPDVGMEQETSYSKEF--TPMPLQKQQSAKPKNHYHPSGGEFAKTTAYKSEY--IYNGGEQSQSFKPKRTYSPPALPMEQRTSYNEEFI------RRIHEK 200          
BLAST of SMED30033381 vs. TrEMBL
Match: A0A183JY31 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS7631 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 5.613e-15
Identity = 90/372 (24.19%), Postives = 159/372 (42.74%), Query Frame = 2
Query:  278 KQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVS----------------------------------TNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDF-------------------------------TNFKTSNLTKN--FKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            +  +N + ++ +  D F ++Y +DY       +  P  PL+Q E   EN    + Y +++   K+ KV   +P   Y  P E M  ++ Y  D+  +     +  KP D++V  S    N++                                  T+Y TD+  K  + +  F+P   Y+   +   S T+YQ ++  +PQS   ++ P   Y+ P  PF +NT+YS +F                               ++++  ++ K+  F P++  H P      +++Y + F +   +  KL   K K     SDE   + +TY+ DF+ ++  PE + S+KPT  Y SP+ P+ +QSSY  DFI
Sbjct:  106 RNAENYDNLNPTVADPFLTSYTDDYKPYGKFYREDPITPLSQWETPHENDLLESTYQDDYQPYKLDKVNDYRPREFYLPPQEPMETNTTYNLDYPPRYSQMERSFKPYDAYVPPSVPMSNITSYQLEYEDPPWSPKEIPFRMKDHETFPRGNFQSETSYITDYPPKVAQPVHCFKPYREYRTPTEPMSSQTSYQTDYTFQPQSKPTSYSPVYVYQQPKVPFQNNTSYSEEFQPHLFSKPESFKPVYEYRKPRMKMENTTAYSSDYRPWHIKKDDPFYPQDNIHLPRANNEMITSYMDEFPLRYTQ--KLHPIKQKENRWLSDEALSSQTTYLTDFRPVT--PEPSMSYKPTEIYRSPSLPVVSQSSYAEDFI 473          

HSP 2 Score: 69.707 bits (169), Expect = 9.301e-9
Identity = 76/327 (23.24%), Postives = 140/327 (42.81%), Query Frame = 2
Query:  329 KSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVS---------------------------------TNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDE-PFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSL 1207
            +++Y+ DYT +   +  S + P+   +  K  F   T+Y+EEF      K +S KP+ +Y++P  KM   + Y SD+   +  ++    PQD+  L   +NE ++                                 T Y TDF     E   S++P +IY+      +S ++Y  +F+ R         PK + ++   P    ++YS +F  ++ +  T+N KP   +  P +PF+  ++YQ  F              P  EYK +   P  T  +Y+Y   +++     T  F+P  N  S  +P+  + + + +++ N  L
Sbjct:  295 QTSYQTDYTFQPQSKPTS-YSPVYVYQQPKVPFQNNTSYSEEFQPHLFSKPESFKPVYEYRKPRMKMENTTAYSSDYRPWHIKKDDPFYPQDNIHLPRANNEMITSYMDEFPLRYTQKLHPIKQKENRWLSDEALSSQTTYLTDFRPVTPEPSMSYKPTEIYRSPSLPVVSQSSYAEDFIPRYAKKQDPLLPKSNLKVSKLPMDKTSSYSEEF-QYRKAKRTENLKPIYRYKHPSVPFSGKTSYQLEFP------------PPSREYKGNRSCPPTTRYSYMYPATRMNTTTSYTDDFRPIKN-PSKLQPIVKEDNLKINYLINNDL 606          
BLAST of SMED30033381 vs. TrEMBL
Match: G4V950 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_034680 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.332e-15
Identity = 95/372 (25.54%), Postives = 161/372 (43.28%), Query Frame = 2
Query:  275 IKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVS----------------------------------TNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQ---VTKEKP------IKLP------------SFKPKSEYK-----------FSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            ++  DN N ++    D F ++Y EDY       +  P  PL+Q E   +N    + Y +++   K+ KV   +P   Y  P E +  ++ Y  D+  +     K  KP +++V  S    N++                                  T+Y TD+  K  + + SF+P   Y+   +  +S T+YQ +++ +P+S   ++ P   Y+ P  PF  NT+YS +F     S   ++FKP   +  P +   +++ Y   ++   V K+ P      I LP             F P+   K            SDE   + +TY+ DF+ ++  PE + S+KPT  Y SP+ P+ +QSSY  DFI
Sbjct:  108 VENYDNLNRMA---ADPFLTSYTEDYKPYGKFHREDPIIPLSQWESPHDNDLLDSTYQDDYQPYKLDKVNDYRPREFYLPPQEPVDTNTTYNLDYPPRYSQMEKSFKPYEAYVPPSVPMSNITSYQLEYEDPPWSPKEMPFRMKDHETFPKGKFQGETSYNTDYPPKVAQPVHSFKPYRKYRTPTEPMLSQTSYQTDYIFQPRSKPTSYSPVYVYQQPKVPFQKNTSYSEEFQPHLFSK-PESFKPVYEYRKPRMKMENITAYSSDYRPWHVKKDGPFYPKDNIYLPRVNNEMITSYMDEFPPRYTQKLYPIKQKENRWLSDEALSSQTTYLNDFRPVT--PEPSMSYKPTEVYRSPSLPVVSQSSYAEDFI 473          
BLAST of SMED30033381 vs. Ensembl Cavefish
Match: saxo2 (stabilizer of axonemal microtubules 2 [Source:NCBI gene;Acc:103022203])

HSP 1 Score: 58.5362 bits (140), Expect = 9.610e-9
Identity = 71/286 (24.83%), Postives = 120/286 (41.96%), Query Frame = 2
Query:  380 SPFKPLNQIEMSKENFDGLTNYNEEF-VNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANK-SLPRNIHKKL 1231
             P +P        E +  +T Y E+F  ++     K LKP  +Y     KM   + +KSDF      R   K  Q +    +    ++ T Y  DF+    + +   +P  + +PT     ++  Y+ +F        +  KP  +Y  P   F + T +  DF   +   L ++FKP N     D PF  +++ Q  +      P++    K   EYK S +PF   +T+  D+Q +S      +SFKP     S   P  + + ++  F     SLP+ +HK +
Sbjct:   17 CPHQPTVLYRKGSEGY-AVTEYVEKFPAHESRQPPKILKPKYEYTADRGKMEGTTTFKSDFIPYEVSRRPGK--QQAEYQPNPGEIDLGTTYKQDFNPYQLQPVTPLKPKVMIRPTSGKLNAVPTYKEDFRAWEIPKRELSKPDETYHPPPGKFGNTTTFQDDFVP-RGPVLRESFKPLNVAKQNDAPFEGVTSNQLSY---IPHPVEARFIKAPEEYKPSSQPFQDLTTHRKDYQGISG--PMPKSFKPERVKISSEAPFQSSTEFRERFQQWPVSLPQ-VHKSV 292          

HSP 2 Score: 52.7582 bits (125), Expect = 5.898e-7
Identity = 49/202 (24.26%), Postives = 91/202 (45.05%), Query Frame = 2
Query:  425 FDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEY 1030
             D  T Y ++F   ++  V  LKP +  +  S K+     YK DF      + ++ KP +++        N +T +  DF  +     +SF+P ++ K  +     +T+ Q+ ++  P    +  K    Y+  ++PF   T +  D+    +  + K+FKP+    S + PF S + ++E FQ   + P+ LP      EY
Sbjct:   99 IDLGTTYKQDFNPYQLQPVTPLKPKVMIRPTSGKLNAVPTYKEDFRAWEIPKRELSKPDETYHPPPGKFGN-TTTFQDDFVPRGPVLRESFKPLNVAKQNDAPFEGVTSNQLSYIPHPVE-ARFIKAPEEYKPSSQPFQDLTTHRKDYQGI-SGPMPKSFKPERVKISSEAPFQSSTEFRERFQ---QWPVSLPQVHKSVEY 294          
BLAST of SMED30033381 vs. Planmine SMEST
Match: SMESG000022853.1 (SMESG000022853.1)

HSP 1 Score: 752.666 bits (1942), Expect = 0.000e+0
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 2
Query:  209 MSNLSKYSSEDISPRSYSKLPSIKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQSEQQLGIFMPNIGQNVPKIPSDPKRDEQKLKSFSLDK 1351
            MSNLSKYSSEDISPRSYSKLPSIKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQSEQQLGIFMPNIGQNVPKIPSDPKRDEQKLKSFSLDK
Sbjct:    1 MSNLSKYSSEDISPRSYSKLPSIKQKDNGNIISNSHCDTFKSAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQSEQQLGIFMPNIGQNVPKIPSDPKRDEQKLKSFSLDK 381          
BLAST of SMED30033381 vs. Planmine SMEST
Match: SMESG000042230.1 (SMESG000042230.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.646e-11
Identity = 66/276 (23.91%), Postives = 132/276 (47.83%), Query Frame = 2
Query:  434 LTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEK----TRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQSEQ 1249
            L+ Y  ++   +I  V S KP+  Y +  E +   +N++ D+     ++  + + ++ +  S  + E  +T YT++F+ K  EK   F+ N   +   + E + T Y+ ++     SP +       +  P+E F+    YSSD+  +K++   ++F+P      P++PF  ++  +E +    + P++    K +SE+     PF   ST+  DF+      EK     RS+KP +     +EPL  ++++ +D+      P  + K   +QSE+
Sbjct:   35 LSEYTNKYTPHQINPVHSFKPLANYTRNPEPLEDKTNHRIDYVPHALEKKYVHQ-REPYKPSEGEFEK-NTTYTSEFNPKHGEKAMMFKRNPERRELGKFEGTPT-YKADYPGWLASPREVHGKPAEWIPPSETFNGIPTYSSDYVKYKSTKPRESFRPHQETQIPNVPFQDMTENRESY---IQHPVEQRKIKDQSEWFRPVVPFTGESTFSSDFK------EKPLGLNRSYKPPAIAKLSSEPLQNETTHNSDY-----KPWGVEKPYLHQSER 293          

HSP 2 Score: 56.6102 bits (135), Expect = 2.393e-8
Identity = 64/253 (25.30%), Postives = 111/253 (43.87%), Query Frame = 2
Query:  338 YREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLP-----SFKPKSEYKFSDEPFDTTSTYVYDF 1081
            Y  DY   +S +    F+P  + ++    F  +T   E ++ +  V+ + +K   ++ +P       S + SDF  K    N+  KP     LSS+  +N  T + +D+     EK      ++ Y P E   ++ T Y  +FL R       F+PK + E   + F S T+YS DF  +  +   + F P   +   DIPF + + +   F       + +P     SFK  ++   S+EPF+  S Y  ++
Sbjct:  172 YSSDYVKYKSTKPRESFRPHQETQIPNVPFQDMTENRESYI-QHPVEQRKIKDQSEWFRPVVPFTGESTFSSDFKEKPLGLNRSYKPPAIAKLSSEPLQN-ETTHNSDYKPWGVEK-PYLHQSERYNPPEGSMMTDTTYLRDFLPRRTERVLAFRPKDNRESIGQ-FSSKTSYSDDFQKWNIA-ARQRFGPNREYIPSDIPFENRTTHGTHF-------VPMPLSIRQSFKQSNQTHLSNEPFNGESIYRTEY 412          
BLAST of SMED30033381 vs. Planmine SMEST
Match: SMESG000067500.1 (SMESG000067500.1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.396e-8
Identity = 63/274 (22.99%), Postives = 112/274 (40.88%), Query Frame = 2
Query:  419 ENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKS---------------------------------DFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISL-TNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKP--KNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTS 1132
            + FDG+ +Y  +++       KS KP++ + Q ++ +A  ++Y+                                  ++T K     K  KP   F+  +D   +  T +  DF   D +K   + P    KP  + +I L T Y  +++  P   T + K K S ++ + PF   TNY  DF N+    +    +P  ++ +  P  PF   + ++  F     +  K+ SFKP + +  S EPFD  + Y       + +  K R + P S
Sbjct:  375 DKFDGIPSYRRDYIPHCQAPPKSFKPVVSFMQSTDPLAQDTDYRQSYVNHPLEPRIKKKEPCFLQNRVPFDALTTHRKEYTPKEICPQKSYKPAPHFI-QADKPLSDETTHRVDFKIHDVDKPFKYEPEKYRKP--EGDIDLKTTYNKDYINLPACKTVSVKEKPSRKV-SAPFQDGTNYRDDFKNWDVPPV----RPIRRDIYEPPTDPFEDCTTFKSAF--VPHELCKVDSFKP-APHMLSQEPFDDATMY------RTEYTNKCRPYCPAS 631          

HSP 2 Score: 51.2174 bits (121), Expect = 1.699e-6
Identity = 44/179 (24.58%), Postives = 91/179 (50.84%), Query Frame = 2
Query:  659 DFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNP-FHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            D+   D       +P+D +K T++ +  + +Y+ +++   Q+P ++FKP +S+   T+P   +T+Y   + N     L    K K P F    +PF++L+ +++ +   +  P K  S+KP   +  +D+P    +T+  DF K+ +  +K   ++P   Y  P   +  +++Y  D+I
Sbjct:  353 DYIPWDIPPATRRKPDDYFKNTDKFD-GIPSYRRDYIPHCQAPPKSFKPVVSFMQSTDPLAQDTDYRQSYVNHP---LEPRIKKKEPCFLQNRVPFDALTTHRKEYTPKEICPQK--SYKPAPHFIQADKPLSDETTHRVDF-KIHDV-DKPFKYEP-EKYRKPEGDIDLKTTYNKDYI 522          
BLAST of SMED30033381 vs. Planmine SMEST
Match: SMESG000067510.1 (SMESG000067510.1)

HSP 1 Score: 55.0694 bits (131), Expect = 8.672e-8
Identity = 62/274 (22.63%), Postives = 111/274 (40.51%), Query Frame = 2
Query:  419 ENFDGLTNYNEEFVNKKIVKVKSLKPIIQYQQPSEKMAFHSNYKS---------------------------------DFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISL-TNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKP--KNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTS 1132
            + FDG+ +Y  +++       KS KP++ + Q ++ +A  ++Y+                                  ++T K     K  KP   F+  +D   +  T +  DF   D +K   + P    KP  + +I L T Y  +++  P   T + K K S ++ + PF   TNY  DF N+    +    +P  ++ +  P  PF   + ++  F     +  K+ SFK  + +  S EPFD  + Y       + +  K R + P S
Sbjct:  154 DKFDGIPSYRRDYIPHCQAPPKSFKPVVSFMQSTDPLAQDTDYRQSYVNHPLEPRIKKKEPCFLQNRVPFDALTTHRKEYTPKEICPQKSYKPAPHFI-QADKPLSDETTHRVDFKIHDVDKPFKYEPEKYRKP--EGDIDLKTTYNKDYINLPACKTVSVKEKPSRKV-SAPFQDGTNYRDDFKNWDVPPV----RPIRRDIYEPPTDPFEDCTTFKSAF--VPHELCKVDSFK-TAPHMLSQEPFDDATMY------RTEYTNKCRPYCPAS 410          

HSP 2 Score: 49.2914 bits (116), Expect = 5.993e-6
Identity = 44/179 (24.58%), Postives = 91/179 (50.84%), Query Frame = 2
Query:  659 DFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNP-FHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFI 1192
            D+   D       +P+D +K T++ +  + +Y+ +++   Q+P ++FKP +S+   T+P   +T+Y   + N     L    K K P F    +PF++L+ +++ +   +  P K  S+KP   +  +D+P    +T+  DF K+ +  +K   ++P   Y  P   +  +++Y  D+I
Sbjct:  132 DYIPWDIPPATRRKPDDYFKNTDKFD-GIPSYRRDYIPHCQAPPKSFKPVVSFMQSTDPLAQDTDYRQSYVNHP---LEPRIKKKEPCFLQNRVPFDALTTHRKEYTPKEICPQK--SYKPAPHFIQADKPLSDETTHRVDF-KIHDV-DKPFKYEP-EKYRKPEGDIDLKTTYNKDYI 301          
BLAST of SMED30033381 vs. Planmine SMEST
Match: SMESG000072576.1 (SMESG000072576.1)

HSP 1 Score: 53.1434 bits (126), Expect = 3.543e-7
Identity = 75/295 (25.42%), Postives = 121/295 (41.02%), Query Frame = 2
Query:  332 SAYREDYTIKRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQ-YQQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDN---ENVSTNYTTDFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPKISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSNYQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEKTRSFKPTSNYSSPTEPLSTQSSYQADFIANKS 1204
            S Y   Y  + S    S   P N I +S      +T    +FV   +  V  LK   Q Y+ P   M   S Y+ +FT K    N++ +P      ++ DN   E+ +TN TT            +  +D+  P             E   R Q           Y+ PTEPF +   Y SDF  +  + ++    P     S D PFNS+S  +  +     +P ++     K  Y  SDEPF++ +T   DF  + N   K++  KP  +     +P ++ ++ + D++A+ S
Sbjct:   36 SEYGGQYHARPSERPESCKPPYNPI-LSDCPLSTVTTNRADFVAHDVHPV--LKREHQKYEPPCTPMNDQSVYRHEFTEKPHCANRMIRPP----ATTQDNGKFESATTNRTT------------YVAHDVCPP-------------ELRSREQ-----------YKPPTEPFDATNTYRSDFIPYDQAKMSSCKPPVTSLKS-DAPFNSVSEQRHSYVQHNVEPRQIAE---KIPYVKSDEPFESMTTNRQDFVPMENC--KSQLMKPPGHRVECDKPFASDTTNRTDYVAHPS 281          
The following BLAST results are available for this feature:
BLAST of SMED30033381 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SAXO16.135e-724.09stabilizer of axonemal microtubules 1 [Source:HGNC... [more]
back to top
BLAST of SMED30033381 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
saxo21.697e-620.07stabilizer of axonemal microtubules 2 [Source:ZFIN... [more]
back to top
BLAST of SMED30033381 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Saxo15.885e-726.92stabilizer of axonemal microtubules 1 [Source:MGI ... [more]
back to top
BLAST of SMED30033381 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q8IYX7|SAXO1_HUMAN2.946e-624.09Stabilizer of axonemal microtubules 1 OS=Homo sapi... [more]
sp|Q4R7D3|SAXO1_MACFA3.300e-624.09Stabilizer of axonemal microtubules 1 OS=Macaca fa... [more]
back to top
BLAST of SMED30033381 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183WTP78.589e-1627.53Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A183QDV91.242e-1525.54Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A3S5B7S92.484e-1528.13Uncharacterized protein (Fragment) OS=Protopolysto... [more]
A0A183JY315.613e-1524.19Uncharacterized protein OS=Schistosoma curassoni O... [more]
G4V9506.332e-1525.54Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
back to top
BLAST of SMED30033381 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
saxo29.610e-924.83stabilizer of axonemal microtubules 2 [Source:NCBI... [more]
back to top
BLAST of SMED30033381 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30033381 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022853.10.000e+0100.00SMESG000022853.1[more]
SMESG000042230.11.646e-1123.91SMESG000042230.1[more]
SMESG000067500.12.396e-822.99SMESG000067500.1[more]
SMESG000067510.18.672e-822.63SMESG000067510.1[more]
SMESG000072576.13.543e-725.42SMESG000072576.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033381 ID=SMED30033381|Name=SMED30033381|organism=Schmidtea mediterranea sexual|type=transcript|length=1568bp
GTCGTTTCAAGGCAAATTCTGTTCTTTTTCTTTACTTACAAACGGGACAA
TTTCTTCTCACAAATATTAGTCGACATGCATAATTTATCGATTTTCTTTG
TTTAACTAAGCTTGTTTAACAAATCCAATTCAAAAAATAATGGATTAATA
GGAAAATGAAAACCCAGAGAATTATTTGTGCATTTTCAAGATACCACATA
TCTATTTAATGTCAAATTTATCAAAATATTCTTCTGAAGATATTTCTCCG
AGAAGTTATTCAAAATTACCTTCAATTAAACAAAAAGATAATGGGAATAT
AATTTCAAATAGTCACTGTGACACTTTTAAAAGTGCCTACCGAGAAGACT
ACACAATCAAAAGAAGTATGGAAAAAGTGTCACCTTTCAAACCACTCAAT
CAAATTGAGATGAGTAAAGAAAACTTTGATGGACTAACTAATTACAACGA
AGAATTTGTTAACAAAAAAATAGTTAAAGTGAAATCTCTTAAACCTATAA
TTCAGTACCAACAACCATCTGAAAAAATGGCTTTCCATTCAAACTATAAA
TCTGATTTCACTTTGAAAAACTTTGATAGAAATAAAATTAAAAAGCCCCA
GGATTCTTTTGTGCTGTCTAGCGATGACAATGAAAATGTTTCCACCAATT
ATACAACAGATTTTTCTAAAAAGGACTTTGAAAAAATTCAAAGTTTTCGT
CCAAATGATATCTATAAACCAACTGAACAAGATGAAATTTCACTTACCAA
CTACCAAATGGAATTCCTAAAAAGACCTCAATCTCCAACACAGAATTTCA
AACCGAAAATCTCATATGAAATCCCAACAGAACCATTTCATTCAAACACA
AATTACAGTTCTGATTTTACAAATTTCAAGACTTCAAATTTAACAAAAAA
TTTCAAACCTAAAAACCCTTTTCACTCTCCGGATATTCCATTTAATAGTC
TGTCAAATTACCAGGAAGGGTTTCAAGTGACCAAAGAAAAGCCCATTAAA
CTACCGAGTTTTAAACCGAAATCCGAATATAAATTCTCGGATGAGCCATT
TGATACAACATCTACGTATGTTTATGATTTTCAAAAATTATCAAATTTTC
CCGAAAAGACTCGTAGCTTTAAACCAACATCAAATTATAGCTCTCCTACA
GAGCCTTTAAGCACCCAATCAAGCTATCAAGCCGATTTTATTGCGAATAA
ATCTTTACCAAGAAATATTCACAAGAAATTAGCCAATCAAAGTGAACAAC
AATTAGGGATATTTATGCCAAATATAGGACAAAATGTTCCGAAAATCCCA
TCCGACCCAAAACGAGATGAACAAAAACTGAAATCGTTTTCGTTGGATAA
ATAAACGAATCTGTTCAAATTTATCCAAGCTTTAAAAACGATCAAGAATT
TCCATTAAAAATAAAAAACATGTTTTAATTTGGTAATTCGAAAGATCAAA
ACTATTTCATTTAAACAAATCCAATTATAAAAATATTTAGTTTTAATTGC
CATTCGCGTTATTTTTTAAACGAAATTCGTAAATACGTTTAAAATTGTTT
CGGAAATTTTGGTTTAAT
back to top

protein sequence of SMED30033381-orf-1

>SMED30033381-orf-1 ID=SMED30033381-orf-1|Name=SMED30033381-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=382bp
MSNLSKYSSEDISPRSYSKLPSIKQKDNGNIISNSHCDTFKSAYREDYTI
KRSMEKVSPFKPLNQIEMSKENFDGLTNYNEEFVNKKIVKVKSLKPIIQY
QQPSEKMAFHSNYKSDFTLKNFDRNKIKKPQDSFVLSSDDNENVSTNYTT
DFSKKDFEKIQSFRPNDIYKPTEQDEISLTNYQMEFLKRPQSPTQNFKPK
ISYEIPTEPFHSNTNYSSDFTNFKTSNLTKNFKPKNPFHSPDIPFNSLSN
YQEGFQVTKEKPIKLPSFKPKSEYKFSDEPFDTTSTYVYDFQKLSNFPEK
TRSFKPTSNYSSPTEPLSTQSSYQADFIANKSLPRNIHKKLANQSEQQLG
IFMPNIGQNVPKIPSDPKRDEQKLKSFSLDK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR033336SAXO1/2
Vocabulary: molecular function
TermDefinition
GO:0008017microtubule binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 356..381
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..381
NoneNo IPR availablePANTHERPTHR31516:SF13STABILIZER OF AXONEMAL MICROTUBULES 2coord: 41..226
coord: 167..337
IPR033336Stabilizer of axonemal microtubules 1/2PANTHERPTHR31516FAMILY NOT NAMEDcoord: 41..226
coord: 167..337