40S ribosomal protein S29

Overview
Name40S ribosomal protein S29
Smed IDSMED30033336
Length (bp)645
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S29 (SMED30033336) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S29 (SMED30033336) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S29 (SMED30033336) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S29 (SMED30033336) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033336

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033336 SmedASXL_078966SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
body wall musculatureSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30033336 dd_Smed_v4_214_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 79.337 bits (194), Expect = 2.912e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 79.337 bits (194), Expect = 2.912e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 78.1814 bits (191), Expect = 1.057e-18
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 78.5666 bits (192), Expect = 1.222e-18
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 74.3294 bits (181), Expect = 1.883e-17
Identity = 33/43 (76.74%), Postives = 39/43 (90.70%), Query Frame = 2 
Query:  143 SHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    2 SHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 44
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Celegans
Match: rps-29 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:P90983])

HSP 1 Score: 75.485 bits (184), Expect = 3.289e-18
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M   N+W SHP+ +G GSR CRVC+  HGL+RKYGL++CRRCFRE A  IG
Sbjct:    1 MGFQNLWFSHPRKFGPGSRSCRVCAGHHGLIRKYGLDLCRRCFREQARDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Fly
Match: RpS29 (gene:FBgn0261599 transcript:FBtr0082136)

HSP 1 Score: 80.8777 bits (198), Expect = 2.934e-20
Identity = 36/51 (70.59%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M  A +W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGFATLWYSHPRKYGQGSRCCRACSNRHGLIRKYGLNICRQCFREYANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Fly
Match: RpS29 (gene:FBgn0261599 transcript:FBtr0334681)

HSP 1 Score: 80.8777 bits (198), Expect = 2.934e-20
Identity = 36/51 (70.59%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M  A +W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGFATLWYSHPRKYGQGSRCCRACSNRHGLIRKYGLNICRQCFREYANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Zebrafish
Match: rps29 (ribosomal protein S29 [Source:ZFIN;Acc:ZDB-GENE-040622-5])

HSP 1 Score: 80.4925 bits (197), Expect = 6.718e-20
Identity = 36/51 (70.59%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ YGQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKYGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Xenopus
Match: rps29 (ribosomal protein S29 [Source:NCBI gene;Acc:549520])

HSP 1 Score: 79.337 bits (194), Expect = 2.639e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Mouse
Match: Rps29 (ribosomal protein S29 [Source:MGI Symbol;Acc:MGI:107681])

HSP 1 Score: 79.337 bits (194), Expect = 1.999e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Match: sp|Q6F473|RS29_PLUXY (40S ribosomal protein S29 OS=Plutella xylostella OX=51655 GN=RpS29 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.211e-21
Identity = 38/51 (74.51%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGHANIWYSHPRKYGQGSRSCRACSNRHGLIRKYGLNICRQCFREYANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Match: sp|Q8WQI3|RS29_SPOFR (40S ribosomal protein S29 OS=Spodoptera frugiperda OX=7108 GN=RpS29 PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.941e-21
Identity = 38/51 (74.51%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGHANIWYSHPRRYGQGSRSCRACSNRHGLIRKYGLNICRQCFREYAHDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Match: sp|Q5UAL3|RS29_BOMMO (40S ribosomal protein S29 OS=Bombyx mori OX=7091 GN=RpS29 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.615e-21
Identity = 38/51 (74.51%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGHANIWYSHPRRYGQGSRSCRSCSNRHGLIRKYGLNICRQCFREYAHDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Match: sp|Q4LAY1|RS29_SCALT (40S ribosomal protein S29 OS=Scarabaeus laticollis OX=292456 GN=RpS29 PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 7.574e-21
Identity = 37/51 (72.55%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H N+W+SHP+ YGQGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGHQNIWYSHPRKYGQGSRSCRACSNRHGLIRKYGLNICRQCFREYANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Match: sp|Q5MGJ4|RS29_LONON (40S ribosomal protein S29 OS=Lonomia obliqua OX=304329 GN=RpS29 PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.246e-20
Identity = 37/51 (72.55%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+W+SHP+ Y QGSR CR CSNRHGL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MGHANIWYSHPRRYCQGSRSCRACSNRHGLIRKYGLNICRQCFREYAHDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. TrEMBL
Match: Q5BTL1 (Ribosomal protein S29 OS=Schistosoma japonicum OX=6182 GN=rps29 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.344e-21
Identity = 42/51 (82.35%), Postives = 47/51 (92.16%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+WHSHPKNYGQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A +IG
Sbjct:    1 MGHANIWHSHPKNYGQGSRCCRVCSNRHGLIRKYGLNMCRQCFRQYASVIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. TrEMBL
Match: A0A4V3SF80 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004799 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.559e-21
Identity = 41/51 (80.39%), Postives = 46/51 (90.20%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+WHSHPK+YGQGSR CRVCSNRHGL+RKYGLNMCR+CFR  A +IG
Sbjct:    1 MGHANIWHSHPKDYGQGSRHCRVCSNRHGLIRKYGLNMCRQCFRMYANVIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. TrEMBL
Match: G7YKF2 (Small subunit ribosomal protein S29e OS=Clonorchis sinensis OX=79923 GN=CLF_110211 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.559e-21
Identity = 41/51 (80.39%), Postives = 46/51 (90.20%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+WHSHPK+YGQGSR CRVCSNRHGL+RKYGLNMCR+CFR  A +IG
Sbjct:    1 MGHANIWHSHPKDYGQGSRHCRVCSNRHGLIRKYGLNMCRQCFRMYANVIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. TrEMBL
Match: A0A5J4NRT6 (Small subunit ribosomal protein S29e OS=Paragonimus westermani OX=34504 GN=DEA37_0003138 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.682e-20
Identity = 41/51 (80.39%), Postives = 45/51 (88.24%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H N+WHSHPKNYGQGSR CRVCSNRHGL+RKYGLNMCR+CFR  A +IG
Sbjct:    1 MGHDNIWHSHPKNYGQGSRLCRVCSNRHGLIRKYGLNMCRQCFRMYANVIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. TrEMBL
Match: A0A183WK39 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS12492 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.756e-20
Identity = 40/51 (78.43%), Postives = 47/51 (92.16%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M HAN+WHSHPKNYGQGSR CRVC+NRHGL+RKYGL+MCR+CFR+ A +IG
Sbjct:    1 MGHANIWHSHPKNYGQGSRCCRVCANRHGLIRKYGLHMCRQCFRQYASVIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Cavefish
Match: ENSAMXT00000021180.2 (pep primary_assembly:Astyanax_mexicanus-2.0:3:43360527:43362955:1 gene:ENSAMXG00000020569.2 transcript:ENSAMXT00000021180.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.9518 bits (193), Expect = 2.526e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Cavefish
Match: ENSAMXT00000053049.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:43359275:43362955:1 gene:ENSAMXG00000020569.2 transcript:ENSAMXT00000053049.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 1.126e-10
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 2 
Query:  152 KNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            +N  +G  + RVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:   12 ENSEKGHLEDRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Yeast
Match: RPS29A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication [Source:SGD;Acc:S000004380])

HSP 1 Score: 80.8777 bits (198), Expect = 4.374e-21
Identity = 36/51 (70.59%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H NVW SHP+ YG+GSRQCRVCS+  GL+RKYGLN+CR+CFRE A  IG
Sbjct:    1 MAHENVWFSHPRRYGKGSRQCRVCSSHTGLIRKYGLNICRQCFREKANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Yeast
Match: RPS29B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication [Source:SGD;Acc:S000002219])

HSP 1 Score: 78.1814 bits (191), Expect = 5.606e-20
Identity = 35/51 (68.63%), Postives = 41/51 (80.39%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H NVW SHP+ +G+GSRQCRVCS+  GLVRKY LN+CR+CFRE A  IG
Sbjct:    1 MAHENVWFSHPRRFGKGSRQCRVCSSHTGLVRKYDLNICRQCFREKANDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Nematostella
Match: EDO42174 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S249])

HSP 1 Score: 83.1889 bits (204), Expect = 1.999e-21
Identity = 37/51 (72.55%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H+NVW SHP++YG GSR CRVCSN HGL+RKYGLN+CRRCFR+ A+ IG
Sbjct:    1 MGHSNVWFSHPRSYGPGSRSCRVCSNHHGLIRKYGLNICRRCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Medaka
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 80.1073 bits (196), Expect = 1.147e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
[Close]
BLAST of 40S ribosomal protein S29 vs. Ensembl Medaka
Match: RPS29 (ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC:10419])

HSP 1 Score: 79.337 bits (194), Expect = 1.594e-19
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 2 
Query:  119 MPHANVWHSHPKNYGQGSRQCRVCSNRHGLVRKYGLNMCRRCFRENAEMIG 271
            M H  ++ SHP+ +GQGSR CRVCSNRHGL+RKYGLNMCR+CFR+ A+ IG
Sbjct:    1 MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIG 51
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S29 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPS292.912e-1968.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS292.912e-1968.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS291.057e-1868.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS291.222e-1868.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS291.883e-1776.74ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps-293.289e-1862.75Ribosomal Protein, Small subunit [Source:UniProtK... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RpS292.934e-2070.59gene:FBgn0261599 transcript:FBtr0082136[more]
RpS292.934e-2070.59gene:FBgn0261599 transcript:FBtr0334681[more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps296.718e-2070.59ribosomal protein S29 [Source:ZFIN;Acc:ZDB-GENE-04... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps292.639e-1968.63ribosomal protein S29 [Source:NCBI gene;Acc:549520... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rps291.999e-1968.63ribosomal protein S29 [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of 40S ribosomal protein S29 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6F473|RS29_PLUXY1.211e-2174.5140S ribosomal protein S29 OS=Plutella xylostella O... [more]
sp|Q8WQI3|RS29_SPOFR1.941e-2174.5140S ribosomal protein S29 OS=Spodoptera frugiperda... [more]
sp|Q5UAL3|RS29_BOMMO3.615e-2174.5140S ribosomal protein S29 OS=Bombyx mori OX=7091 G... [more]
sp|Q4LAY1|RS29_SCALT7.574e-2172.5540S ribosomal protein S29 OS=Scarabaeus laticollis... [more]
sp|Q5MGJ4|RS29_LONON4.246e-2072.5540S ribosomal protein S29 OS=Lonomia obliqua OX=30... [more]
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BLAST of 40S ribosomal protein S29 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q5BTL11.344e-2182.35Ribosomal protein S29 OS=Schistosoma japonicum OX=... [more]
A0A4V3SF805.559e-2180.39Uncharacterized protein OS=Opisthorchis felineus O... [more]
G7YKF25.559e-2180.39Small subunit ribosomal protein S29e OS=Clonorchis... [more]
A0A5J4NRT61.682e-2080.39Small subunit ribosomal protein S29e OS=Paragonimu... [more]
A0A183WK391.756e-2078.43Uncharacterized protein OS=Trichobilharzia regenti... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000021180.22.526e-1968.63pep primary_assembly:Astyanax_mexicanus-2.0:3:4336... [more]
ENSAMXT00000053049.11.126e-1065.00pep primary_assembly:Astyanax_mexicanus-2.0:3:4335... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 40S ribosomal protein S29 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS29A4.374e-2170.59Protein component of the small (40S) ribosomal sub... [more]
RPS29B5.606e-2068.63Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO421741.999e-2172.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 40S ribosomal protein S29 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RPS291.147e-1968.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS291.594e-1968.63ribosomal protein S29 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S29 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033336 ID=SMED30033336|Name=40S ribosomal protein S29|organism=Schmidtea mediterranea sexual|type=transcript|length=645bp
CATTTTATTAACATTTAAATTTGTTTTAATTCACATAACTCATTCAATAA
AAATATATTCAAAAATTTAGTTAGTATATTTTAAAAATTTGATATATTAT
TAAAGTATTTATTCAGAAATGCCGCATGCAAACGTTTGGCACTCACATCC
AAAAAATTATGGTCAAGGAAGTAGACAATGCCGAGTCTGCTCTAATAGAC
ACGGTTTAGTCAGAAAATATGGATTGAACATGTGCAGAAGATGTTTCAGA
GAAAATGCTGAAATGATTGGATTCAAAAAAGCATAATTTATTAATAAAAT
ATAAATTTGGAGTAATTTTTTGATATTCGATTATCGCAATTTGAATAATC
TAACATTTTTGATGTTTCATCTTTAAAAGTGGTTTTATCATACTTTCAAT
TATGTGTTTTCCTTTATTTTTTTATTAAGTATTTCGATGTTGAAATTTTA
ATATTAATTTAGATAGTATTTTTAGTCTCTGTATCATTGGTTTCATGAAG
ACATTTTAAAATGTCAAATTAATCGTTCAATTCTATGATGCTAGCTTGTT
TTAAAGCTAGCTTTGCATGTAGTGGATTTAATTACACATTTTTATTTAAT
GTTCAAACTTCCTATTTATATTAAATTTATCGTTTCTAAATTTAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0002181cytoplasmic translation
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005791rough endoplasmic reticulum
GO:0005829cytosol
GO:0022627cytosolic small ribosomal subunit
GO:0042788polysomal ribosome
GO:0005840ribosome
GO:0005622intracellular
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0003735structural constituent of ribosome
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000028Category 2 cell
PLANA:0000240body wall musculature
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR043140Ribosomal_S14/S29
IPR03974440S_S29/30S_S14z
IPR001209Ribosomal_S14
IPR018271Ribosomal_S14_CS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043140Ribosomal protein S14/S29GENE3DG3DSA:4.10.830.10coord: 1..55
e-value: 4.1E-33
score: 115.0
IPR001209Ribosomal protein S14PFAMPF00253Ribosomal_S14coord: 7..55
e-value: 3.2E-17
score: 62.0
IPR03974440S ribosomal protein S29/30S ribosomal protein S14 type ZPANTHERPTHR1201040S RIBOSOMAL PROTEIN S29coord: 1..54
IPR018271Ribosomal protein S14, conserved sitePROSITEPS00527RIBOSOMAL_S14coord: 22..44