Cytochrome c oxidase subunit 5B

Overview
NameCytochrome c oxidase subunit 5B
Smed IDSMED30033257
Length (bp)1660
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cytochrome c oxidase subunit 5B (SMED30033257) t-SNE clustered cells

Violin plots show distribution of expression levels for Cytochrome c oxidase subunit 5B (SMED30033257) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cytochrome c oxidase subunit 5B (SMED30033257) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cytochrome c oxidase subunit 5B (SMED30033257) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033257

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30033257SMESG000050948.1 dd_Smed_v4_861_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033257SMESG000050948.1 Contig51515newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Celegans
Match: cox-5B (Cytochrome OXidase assembly protein [Source:UniProtKB/TrEMBL;Acc:P90849])

HSP 1 Score: 53.9138 bits (128), Expect = 1.411e-8
Identity = 34/87 (39.08%), Postives = 42/87 (48.28%), Query Frame = 3 
Query:  171 PDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPN-STIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLV 428
            PD L  A G+ +  LLA L G+  + E  V  R   ST + PN + SH   R + C C +         I K  P RC CGHWFK V
Sbjct:   40 PDPLEHATGREKKMLLARLAGDDRY-EPKVYYRAEASTKQKPNLVPSHYDFRIIGCMCEQDSGHVNFMTIRKGDPKRCECGHWFKGV 125
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Celegans
Match: cox-5B (Cytochrome OXidase assembly protein [Source:UniProtKB/TrEMBL;Acc:P90849])

HSP 1 Score: 53.9138 bits (128), Expect = 1.411e-8
Identity = 34/87 (39.08%), Postives = 42/87 (48.28%), Query Frame = 3 
Query:  171 PDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPN-STIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLV 428
            PD L  A G+ +  LLA L G+  + E  V  R   ST + PN + SH   R + C C +         I K  P RC CGHWFK V
Sbjct:   40 PDPLEHATGREKKMLLARLAGDDRY-EPKVYYRAEASTKQKPNLVPSHYDFRIIGCMCEQDSGHVNFMTIRKGDPKRCECGHWFKGV 125
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Celegans
Match: cox-5B (Cytochrome OXidase assembly protein [Source:UniProtKB/TrEMBL;Acc:P90849])

HSP 1 Score: 53.9138 bits (128), Expect = 1.411e-8
Identity = 34/87 (39.08%), Postives = 42/87 (48.28%), Query Frame = 3 
Query:  171 PDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPN-STIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLV 428
            PD L  A G+ +  LLA L G+  + E  V  R   ST + PN + SH   R + C C +         I K  P RC CGHWFK V
Sbjct:   40 PDPLEHATGREKKMLLARLAGDDRY-EPKVYYRAEASTKQKPNLVPSHYDFRIIGCMCEQDSGHVNFMTIRKGDPKRCECGHWFKGV 125
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Fly
Match: COX5B (gene:FBgn0031830 transcript:FBtr0343157)

HSP 1 Score: 46.2098 bits (108), Expect = 6.667e-6
Identity = 32/88 (36.36%), Postives = 40/88 (45.45%), Query Frame = 3 
Query:  165 VLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLV 428
            ++ D L  A G  + ELL +  G     +  V  R   T ENPN I S    R + C C E +       + K    RC CGHWFKLV
Sbjct:   28 MMNDPLEHATGIEKRELLLKAAGNDNPFDMKVFKRGAGTKENPNLIPSAFDARIVGCICEEDQTYVQWMWLQKGNQKRCECGHWFKLV 115
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Fly
Match: COX5B (gene:FBgn0031830 transcript:FBtr0079288)

HSP 1 Score: 46.2098 bits (108), Expect = 6.667e-6
Identity = 32/88 (36.36%), Postives = 40/88 (45.45%), Query Frame = 3 
Query:  165 VLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLV 428
            ++ D L  A G  + ELL +  G     +  V  R   T ENPN I S    R + C C E +       + K    RC CGHWFKLV
Sbjct:   28 MMNDPLEHATGIEKRELLLKAAGNDNPFDMKVFKRGAGTKENPNLIPSAFDARIVGCICEEDQTYVQWMWLQKGNQKRCECGHWFKLV 115
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Match: A0A5J4NDZ3 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0008840 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.259e-18
Identity = 65/228 (28.51%), Postives = 115/228 (50.44%), Query Frame = 3 
Query:   12 LRTTNAINILRKNICNTAILSEFQY---KKLDLQE-LRKFAFSDPNCGKEVSEHIVLPDGLGEAIGKRRYELLAEL-YGEKAWGEDGVK-LRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWKGIIKSRKQIEHILD 677
            +++   I  LR   C+ A+L  F     + + ++E +RKF   +P+        + LP  + + + K + ELL ++   +  +    ++ +RPNS  ENP  + S+  R+   C C+   +     ++Y+D+PMRC CGHWFKL+  E         REKR+ ++KD  E  +L  +L   ++  N L   GK +++ SP   +++ KM A W+    S  +I   +D
Sbjct:    1 MQSVGVIGGLRA--CSRALLGRFSSTACQNVSMKERMRKFILENPHLAPSSQTSLNLP--VVKGVTKMQQELLDQIEVIDLDFKPSKIRHIRPNSK-ENPLLVGSNLERKVETCLCYPDVHYVRSIVLYRDLPMRCQCGHWFKLIDMER----FEQEREKRWAQIKDEPENAKLLQDLTDSENELNRLMDQGKNLTKWSPGVTQLLEKMAAQWEKYKDSYAKIRRQMD 219
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Match: A0A3R7G0X3 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CSKR_12279s PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.939e-16
Identity = 45/126 (35.71%), Postives = 70/126 (55.56%), Query Frame = 3 
Query:  258 VKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWK 635
            ++L PNS  ENP  +     RR+M C CF   N     ++Y+D+P RC CG+WFKLV  E        ARE+++ ++KD  E  +L  EL + ++  N+L   G+ ++  +  A E+  K+  AWK
Sbjct:   35 LELAPNSR-ENPLLVGGDEERRAMRCMCFPEVNYMRTLVLYRDMPYRCQCGNWFKLVDMER----FEQAREEKWQKIKDEPENAQLLKELAETEEELNQLFQKGRGMTMYTRGATEVTEKLSVAWK 155
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Match: A0A504Z508 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_03043 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.284e-14
Identity = 40/127 (31.50%), Postives = 68/127 (53.54%), Query Frame = 3 
Query:  261 KLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWKGI 641
            +++P ST ENPN + S   R+   C C+   +   + ++Y+D+PMRC CGHWFKL+  +         REK + ++KD  E  +L  +L   ++  N L   GK +   SP A +++  +   W+ +
Sbjct:   48 RIQP-STRENPNLVGSALERQVSTCVCYPDTHYVRYMVLYRDLPMRCQCGHWFKLIDMDR----FEQEREKHWQKIKDEPENAKLLQQLTDTENELNRLMEKGKDIKRTSPGADDLLEALANQWEKL 169
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Match: C4Q310 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_021730.2 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.422e-14
Identity = 47/124 (37.90%), Postives = 65/124 (52.42%), Query Frame = 3 
Query:  273 NSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVS---YEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWK 635
            +ST ENPN +AS N R    C C  H +     ++Y+++P RC CGHWF LV    YEE        REK++N++KD  E  +L       +   N L   GK+++  SP A E+M  M   WK
Sbjct:   87 SSTKENPNLVASSNVRAVQQCHCSPHDHHLKSLVLYRNLPARCFCGHWFLLVDEQRYEEE-------REKKWNKIKDKPENADLLKRFDILEKELNILLAEGKSMTPKSPGADEMMDNMALKWK 203
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Match: C4Q309 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_021730.1 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.465e-14
Identity = 47/124 (37.90%), Postives = 65/124 (52.42%), Query Frame = 3 
Query:  273 NSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVS---YEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWK 635
            +ST ENPN +AS N R    C C  H +     ++Y+++P RC CGHWF LV    YEE        REK++N++KD  E  +L       +   N L   GK+++  SP A E+M  M   WK
Sbjct:   88 SSTKENPNLVASSNVRAVQQCHCSPHDHHLKSLVLYRNLPARCFCGHWFLLVDEQRYEEE-------REKKWNKIKDKPENADLLKRFDILEKELNILLAEGKSMTPKSPGADEMMDNMALKWK 204
[Close]
BLAST of Cytochrome c oxidase subunit 5B vs. Planmine SMEST
Match: SMESG000050948.1 (SMESG000050948.1)

HSP 1 Score: 472.241 bits (1214), Expect = 9.512e-167
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 3 
Query:    3 MALLRTTNAINILRKNICNTAILSEFQYKKLDLQELRKFAFSDPNCGKEVSEHIVLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWKGIIKSRKQIEHILDN 680
            MALLRTTNAINILRKNICNTAILSEFQYKKLDLQELRKFAFSDPNCGKEVSEHIVLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWKGIIKSRKQIEHILDN
Sbjct:    1 MALLRTTNAINILRKNICNTAILSEFQYKKLDLQELRKFAFSDPNCGKEVSEHIVLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYIASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNIINTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAREIMGKMKAAWKGIIKSRKQIEHILDN 226
The following BLAST results are available for this feature:
BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cox-5B1.411e-839.08Cytochrome OXidase assembly protein [Source:UniPr... [more]
cox-5B1.411e-839.08Cytochrome OXidase assembly protein [Source:UniPr... [more]
cox-5B1.411e-839.08Cytochrome OXidase assembly protein [Source:UniPr... [more]
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
COX5B6.667e-636.36gene:FBgn0031830 transcript:FBtr0343157[more]
COX5B6.667e-636.36gene:FBgn0031830 transcript:FBtr0079288[more]
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NDZ31.259e-1828.51Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A3R7G0X37.939e-1635.71Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
A0A504Z5081.284e-1431.50Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
C4Q3101.422e-1437.90Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
C4Q3091.465e-1437.90Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome c oxidase subunit 5B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000050948.19.512e-167100.00SMESG000050948.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033257 ID=SMED30033257|Name=Cytochrome c oxidase subunit 5B|organism=Schmidtea mediterranea sexual|type=transcript|length=1660bp
CTATGGCTTTATTAAGAACTACTAACGCGATTAATATATTACGTAAAAAT
ATATGCAATACTGCTATATTGTCTGAATTTCAATATAAAAAGTTAGACTT
ACAAGAACTTAGAAAATTTGCATTTTCAGATCCCAATTGTGGAAAGGAAG
TTAGTGAACATATTGTTTTACCCGATGGGCTGGGAGAAGCAATTGGCAAG
CGTAGATATGAATTATTAGCAGAACTTTATGGAGAAAAAGCTTGGGGCGA
AGATGGTGTAAAACTTCGACCTAATTCTACTATAGAAAATCCAAATTATA
TTGCAAGTCATAATTGGCGAAGGTCGATGGTCTGCACATGTTTCGAGCAC
AGGAATAGCACCTTCCATCACATGATCTACAAAGACATACCAATGCGTTG
CCTATGTGGCCATTGGTTTAAGTTAGTATCCTATGAGGAACACTATAACA
TCATTAATACGGCGAGAGAAAAGCGATATAATGAACTTAAGGATTCAGCT
GAATTGAAAGAGCTAGAAATGGAATTGAAACAACATCAGGATAATTTCAA
TGAGTTATCATTCAGTGGTAAGGCTGTTAGTGAAGCATCACCAGAGGCAC
GTGAAATAATGGGTAAAATGAAAGCGGCTTGGAAAGGCATAATTAAGTCG
AGGAAACAAATTGAGCATATTCTGGATAACTAGCATCGTGCGCTATTGAA
AATTAATTGAACTTGAGTGCAAAGTCCGGGAATCGATTTAGCGTGTCAAA
TATTAGAATTATTTGTGTAAAATTTATTGATTTTCTTTTAAATAGGTTTA
ATAAATTTCCAACGAGGAGAGGTTGTGAATAATTATACAAATGAAGATTT
TGATAATATCAATGTTGAGTATTGGTCAGATTTTATATTCCACCTTTATC
GATTTTCACTTTATACTATATTTCATTTCTATAGGTTTTTTATTTCATTC
GATAATTTTTCTTATTTTATTGATTTTTCACTTTCAATTAAAAAATCTTT
GGCATATATTTATATTCACATTTTATAGATATTTTACTCCGTTTTATATT
GATTTTTAATTTTCAGTACTTATAAAATAATATATTTTAAATGCTTAACA
CTTAAAATGTTAACTCTTAAAACACAGTCAGTTATTTGCGCAAGTATGAG
TGTGGCATAATGCATTGACCATGTTTTATCTCGATCAAAAAGAATGGAAG
TGGGTTAATTAACACGCAAAATTCATTCAATGCCGAAGAAGTTATAAAGA
ATGTAAAATTGTAAATATAAATTTAAAGTATCAAGTATCGAGAGGTTATG
AATATTTTTTATGTTACTTTTTTATATTTTCGTAATTATTGACATTCACT
TTTTTGCTTCAGTTGGTTTTATTCGAGTACCTTAAATACTTAATGTTTTG
AGCTCCAAATCCATAGTTTTCACATAGAATTTTCATTTCTATTAGAATTA
TTTTTCGTTATTTATAGACTTATGCTGATATTATATATTAAATTTTCGGT
TTATCAAATTCATTCTTAACCTTTTTGCCGCGATGCATTAGTAGTTTTTT
AATTTTTCGCGACAAGTGAACCAAAATCCAGAATGTCTAGTGTCAAATTA
GTCAATGTAAATGCTGTCTGTTTTTGAGAACAAATAAACTGTAAAGAGGT
TCAAAAGTTG
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protein sequence of SMED30033257-orf-1

>SMED30033257-orf-1 ID=SMED30033257-orf-1|Name=SMED30033257-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=227bp
MALLRTTNAINILRKNICNTAILSEFQYKKLDLQELRKFAFSDPNCGKEV
SEHIVLPDGLGEAIGKRRYELLAELYGEKAWGEDGVKLRPNSTIENPNYI
ASHNWRRSMVCTCFEHRNSTFHHMIYKDIPMRCLCGHWFKLVSYEEHYNI
INTAREKRYNELKDSAELKELEMELKQHQDNFNELSFSGKAVSEASPEAR
EIMGKMKAAWKGIIKSRKQIEHILDN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0022900electron transport chain
GO:1902600hydrogen ion transmembrane transport
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036972Cyt_c_oxidase_su5b_sf
IPR002124Cyt_c_oxidase_su5b
Vocabulary: cellular component
TermDefinition
GO:0005740mitochondrial envelope
Vocabulary: molecular function
TermDefinition
GO:0004129cytochrome-c oxidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 161..188
NoneNo IPR availablePRODOMPD007270coord: 63..142
e-value: 2.0E-6
score: 123.0
NoneNo IPR availableSUPERFAMILYSSF57802Rubredoxin-likecoord: 57..146
IPR036972Cytochrome c oxidase, subunit Vb superfamilyGENE3DG3DSA:2.60.11.10coord: 53..148
e-value: 6.0E-15
score: 57.0
IPR002124Cytochrome c oxidase, subunit VbPROSITEPS51359COX5B_2coord: 51..148
score: 13.504