SMED30033255
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033255 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Alignments
SMED30033255 aligns in the following genomic locations:
Homology
BLAST of SMED30033255 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 148.288 bits (373), Expect = 1.485e-42 Identity = 77/82 (93.90%), Postives = 79/82 (96.34%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGE IEKSISNEFIENLNQSNI DSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSIINE Sbjct: 353 DIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINE 434 HSP 2 Score: 127.102 bits (318), Expect = 3.630e-35 Identity = 66/82 (80.49%), Postives = 75/82 (91.46%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN++DKSI NE Sbjct: 451 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNE 532 HSP 3 Score: 120.553 bits (301), Expect = 7.636e-33 Identity = 88/107 (82.24%), Postives = 94/107 (87.85%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINEXXXXXXXXXXXXXXXXXXVDNSSVF 322 +I+ LS +SPV N+ ++PE KSISNEFIE NQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLS KNLSNKSQVDNSSVF Sbjct: 601 EIETLSEQSPVKNSLLLPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVF 701 HSP 4 Score: 108.227 bits (269), Expect = 1.308e-28 Identity = 58/73 (79.45%), Postives = 64/73 (87.67%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQN 220 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+ +N Sbjct: 232 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNEN 304 HSP 5 Score: 103.605 bits (257), Expect = 6.008e-27 Identity = 61/80 (76.25%), Postives = 69/80 (86.25%), Query Frame = 2 Query: 8 QHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 ++LSN+S VDN+SV PE KSISNEFIENLNQS+IIDSVLIN SQTSIRY ESDKTFVLSNAED +NI+D+SIINE Sbjct: 687 KNLSNKSQVDNSSVFPE------KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINE 759 HSP 6 Score: 51.9878 bits (123), Expect = 7.395e-9 Identity = 42/85 (49.41%), Postives = 51/85 (60.00%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDG---QNISDKSIINE 247 +I L+ +S VDN+SVIPE K ISN FIEN NQSNI S+LINK + I S+ N E G NI++KSIINE Sbjct: 844 EILALTEQSQVDNSSVIPE------KPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINE 916
BLAST of SMED30033255 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 148.288 bits (373), Expect = 1.485e-42 Identity = 77/82 (93.90%), Postives = 79/82 (96.34%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGE IEKSISNEFIENLNQSNI DSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSIINE Sbjct: 354 DIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINE 435 HSP 2 Score: 127.102 bits (318), Expect = 3.630e-35 Identity = 66/82 (80.49%), Postives = 75/82 (91.46%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN++DKSI NE Sbjct: 452 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNE 533 HSP 3 Score: 120.553 bits (301), Expect = 7.636e-33 Identity = 88/107 (82.24%), Postives = 94/107 (87.85%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINEXXXXXXXXXXXXXXXXXXVDNSSVF 322 +I+ LS +SPV N+ ++PE KSISNEFIE NQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLS KNLSNKSQVDNSSVF Sbjct: 602 EIETLSEQSPVKNSLLLPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVF 702 HSP 4 Score: 108.227 bits (269), Expect = 1.296e-28 Identity = 58/73 (79.45%), Postives = 64/73 (87.67%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQN 220 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+ +N Sbjct: 233 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNEN 305 HSP 5 Score: 103.605 bits (257), Expect = 6.066e-27 Identity = 61/80 (76.25%), Postives = 69/80 (86.25%), Query Frame = 2 Query: 8 QHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 ++LSN+S VDN+SV PE KSISNEFIENLNQS+IIDSVLIN SQTSIRY ESDKTFVLSNAED +NI+D+SIINE Sbjct: 688 KNLSNKSQVDNSSVFPE------KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINE 760 HSP 6 Score: 51.9878 bits (123), Expect = 7.395e-9 Identity = 42/85 (49.41%), Postives = 51/85 (60.00%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDG---QNISDKSIINE 247 +I L+ +S VDN+SVIPE K ISN FIEN NQSNI S+LINK + I S+ N E G NI++KSIINE Sbjct: 845 EILALTEQSQVDNSSVIPE------KPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINE 917
BLAST of SMED30033255 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 148.288 bits (373), Expect = 1.485e-42 Identity = 77/82 (93.90%), Postives = 79/82 (96.34%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGE IEKSISNEFIENLNQSNI DSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSIINE Sbjct: 358 DIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINE 439 HSP 2 Score: 127.102 bits (318), Expect = 3.560e-35 Identity = 66/82 (80.49%), Postives = 75/82 (91.46%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN++DKSI NE Sbjct: 456 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNE 537 HSP 3 Score: 120.553 bits (301), Expect = 7.563e-33 Identity = 88/107 (82.24%), Postives = 94/107 (87.85%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINEXXXXXXXXXXXXXXXXXXVDNSSVF 322 +I+ LS +SPV N+ ++PE KSISNEFIE NQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLS KNLSNKSQVDNSSVF Sbjct: 606 EIETLSEQSPVKNSLLLPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVF 706 HSP 4 Score: 108.227 bits (269), Expect = 1.271e-28 Identity = 58/73 (79.45%), Postives = 64/73 (87.67%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQN 220 DIQHLSNRSPVDN+SVIPETGEVIEKSISNEFIEN NQSNIIDS LINKSQ+S R +S+KTFVL N+ +N Sbjct: 237 DIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNEN 309 HSP 5 Score: 103.605 bits (257), Expect = 6.185e-27 Identity = 61/80 (76.25%), Postives = 69/80 (86.25%), Query Frame = 2 Query: 8 QHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINE 247 ++LSN+S VDN+SV PE KSISNEFIENLNQS+IIDSVLIN SQTSIRY ESDKTFVLSNAED +NI+D+SIINE Sbjct: 692 KNLSNKSQVDNSSVFPE------KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINE 764 HSP 6 Score: 51.9878 bits (123), Expect = 7.614e-9 Identity = 42/85 (49.41%), Postives = 51/85 (60.00%), Query Frame = 2 Query: 2 DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDG---QNISDKSIINE 247 +I L+ +S VDN+SVIPE K ISN FIEN NQSNI S+LINK + I S+ N E G NI++KSIINE Sbjct: 849 EILALTEQSQVDNSSVIPE------KPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINE 921 The following BLAST results are available for this feature:
BLAST of SMED30033255 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30033255 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30033255 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30033255 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30033255 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033255 ID=SMED30033255|Name=SMED30033255|organism=Schmidtea mediterranea sexual|type=transcript|length=324bpback to top protein sequence of SMED30033255-orf-1 >SMED30033255-orf-1 ID=SMED30033255-orf-1|Name=SMED30033255-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp DIQHLSNRSPVDNNSVIPETGEVIEKSISNEFIENLNQSNIIDSVLINKSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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