TFIIB-type domain-containing protein

Overview


Name	TFIIB-type domain-containing protein
Smed ID	SMED30033223
Length (bp)	1088

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30033223

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
oocyte	SMED30033223	Contig40561	newmark_ests	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30033223	Contig40561	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30033223	Contig40561	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30033223	Contig40561	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017

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Homology

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BLAST of TFIIB-type domain-containing protein vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 69.3218 bits (168), Expect = 1.075e-20
Identity = 63/223 (28.25%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLCLPK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.447 bits (119), Expect = 1.075e-20
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 64.6994 bits (156), Expect = 4.019e-19
Identity = 48/160 (30.00%), Postives = 80/160 (50.00%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVL 714
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L
Sbjct:   43 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL 201

HSP 2 Score: 49.6766 bits (117), Expect = 4.019e-19
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:    9 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 43

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 65.0846 bits (157), Expect = 2.126e-16
Identity = 48/160 (30.00%), Postives = 80/160 (50.00%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVL 714
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L
Sbjct:   39 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL 197

HSP 2 Score: 40.0466 bits (92), Expect = 2.126e-16
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVV 181
            L ++ C  H  A+L+EDY AGD+IC  C  V+
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECDRVI 41

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Celegans
Match: ttb-1 (Transcription initiation factor IIB [Source:UniProtKB/Swiss-Prot;Acc:O16991])

HSP 1 Score: 73.559 bits (179), Expect = 4.221e-20
Identity = 69/223 (30.94%), Postives = 105/223 (47.09%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSF---DAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF---VFKKPSSIMAGTIYMG 885
            R++D   EWRSF+N+  G+D  RVGA E    S    S TI   F   D+ +   N ++K  N       ++ +      I  +++R+ L   I+ SA ++ K + +      KN EA +AACL+  C+++   R  KE  AV+ + K   I +  KIIV+ L  N +       + RF   L + N S++     IAK A        + P SI A  IYM 
Sbjct:   36 RLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSLANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKE-IGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPN-SIQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMA 251

HSP 2 Score: 43.1282 bits (100), Expect = 4.221e-20
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            C  H    LIED+ AGDL+C  C LVVGDR
Sbjct:    7 CPIHPDVHLIEDHRAGDLVCPACGLVVGDR 36

[Close]

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSF---DAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF---VFKKPSSIMAGTIYMG 885
            R++D   EWRSF+N+  G+D  RVGA E    S    S TI   F   D+ +   N ++K  N       ++ +      I  +++R+ L   I+ SA ++ K + +      KN EA +AACL+  C+++   R  KE  AV+ + K   I +  KIIV+ L  N +       + RF   L + N S++     IAK A        + P SI A  IYM 
Sbjct:   36 RLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSLANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKE-IGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPN-SIQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMA 251

HSP 2 Score: 43.1282 bits (100), Expect = 4.221e-20
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            C  H    LIED+ AGDL+C  C LVVGDR
Sbjct:    7 CPIHPDVHLIEDHRAGDLVCPACGLVVGDR 36

[Close]

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSF---DAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF---VFKKPSSIMAGTIYMG 885
            R++D   EWRSF+N+  G+D  RVGA E    S    S TI   F   D+ +   N ++K  N       ++ +      I  +++R+ L   I+ SA ++ K + +      KN EA +AACL+  C+++   R  KE  AV+ + K   I +  KIIV+ L  N +       + RF   L + N S++     IAK A        + P SI A  IYM 
Sbjct:   36 RLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSLANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKE-IGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPN-SIQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMA 251

HSP 2 Score: 43.1282 bits (100), Expect = 4.221e-20
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            C  H    LIED+ AGDL+C  C LVVGDR
Sbjct:    7 CPIHPDVHLIEDHRAGDLVCPACGLVVGDR 36

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Fly
Match: TfIIB (gene:FBgn0004915 transcript:FBtr0080025)

HSP 1 Score: 69.3218 bits (168), Expect = 2.675e-23
Identity = 63/222 (28.38%), Postives = 106/222 (47.75%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYM 882
            RVID  +EWR+F+N+  G D  RVG  E    S    S  I     +      G  K++N   +S +++ + +  + IS++ADR+ L   I   A  + K++        ++ +A ++ACL+  C++    R  KE  AV+ + K   I +  K+ +K L     S ++I     + RF   L + N+ V++    IAK A +      + P S+ A  IYM
Sbjct:   43 RVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKE-IGRCFKLTLKAL---ETSVDLITTADFMCRFCANLDLPNM-VQRAATHIAKKAVEMDIVPGRSPISVAAAAIYM 259

HSP 2 Score: 51.2174 bits (121), Expect = 2.675e-23
Identity = 22/34 (64.71%), Postives = 26/34 (76.47%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +K+CC  H  + LIEDY AGD+ICS C LVVGDR
Sbjct:   10 NKVCCYAHPESPLIEDYRAGDMICSECGLVVGDR 43

HSP 3 Score: 27.335 bits (59), Expect = 2.675e-23
Identity = 11/34 (32.35%), Postives = 20/34 (58.82%), Query Frame = 2

Query:  905 R*QENIKNVVGVSENTTASVYKILFENKESLIPE 1006
            R Q+ I ++ GV++ T    YK+++ +   L PE
Sbjct:  268 RSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPE 301

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Fly
Match: TfIIB (gene:FBgn0004915 transcript:FBtr0333489)

HSP 1 Score: 69.3218 bits (168), Expect = 2.675e-23
Identity = 63/222 (28.38%), Postives = 106/222 (47.75%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYM 882
            RVID  +EWR+F+N+  G D  RVG  E    S    S  I     +      G  K++N   +S +++ + +  + IS++ADR+ L   I   A  + K++        ++ +A ++ACL+  C++    R  KE  AV+ + K   I +  K+ +K L     S ++I     + RF   L + N+ V++    IAK A +      + P S+ A  IYM
Sbjct:   43 RVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKE-IGRCFKLTLKAL---ETSVDLITTADFMCRFCANLDLPNM-VQRAATHIAKKAVEMDIVPGRSPISVAAAAIYM 259

HSP 2 Score: 51.2174 bits (121), Expect = 2.675e-23
Identity = 22/34 (64.71%), Postives = 26/34 (76.47%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +K+CC  H  + LIEDY AGD+ICS C LVVGDR
Sbjct:   10 NKVCCYAHPESPLIEDYRAGDMICSECGLVVGDR 43

HSP 3 Score: 27.335 bits (59), Expect = 2.675e-23
Identity = 11/34 (32.35%), Postives = 20/34 (58.82%), Query Frame = 2

Query:  905 R*QENIKNVVGVSENTTASVYKILFENKESLIPE 1006
            R Q+ I ++ GV++ T    YK+++ +   L PE
Sbjct:  268 RSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPE 301

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Zebrafish
Match: gtf2b (general transcription factor IIB [Source:ZFIN;Acc:ZDB-GENE-030131-3544])

HSP 1 Score: 68.1662 bits (165), Expect = 2.576e-20
Identity = 59/223 (26.46%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+N+    D  RVG  +    +    +  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      ++ +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNEKATKDPSRVGDAQNPLLNGGDLTTMISKGTGAASFDEFGNSKYQNRRTMSSSDRAMLNAFKEITTMADRINLPRNIIDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLGLPK-QVQMAATYIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.0618 bits (118), Expect = 2.576e-20
Identity = 21/35 (60.00%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L K+ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPKVQCPNHPDALLVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Xenopus
Match: rars2 (arginyl-tRNA synthetase 2, mitochondrial [Source:Xenbase;Acc:XB-GENE-976890])

HSP 1 Score: 73.1738 bits (178), Expect = 3.423e-22
Identity = 60/220 (27.27%), Postives = 101/220 (45.91%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    S    +  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      ++ +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L  N         + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   50 RVIDVGSEWRTFSNDKAAKDPSRVGDAQNPLLSGGDLTTMIGKGTGAASFDEFGNNKYQNRRTMSSSDRAMMNAFKEITNMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKALETNVDLITTGDFMSRFCSNLGLTK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 267

HSP 2 Score: 51.9878 bits (123), Expect = 3.423e-22
Identity = 22/35 (62.86%), Postives = 26/35 (74.29%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L K+ C  H  A+L+EDY AGD+ICS C LVVGDR
Sbjct:   16 LPKVTCPNHPDALLVEDYRAGDMICSECGLVVGDR 50

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Xenopus
Match: rars2 (arginyl-tRNA synthetase 2, mitochondrial [Source:Xenbase;Acc:XB-GENE-976890])

HSP 1 Score: 73.1738 bits (178), Expect = 3.481e-22
Identity = 60/220 (27.27%), Postives = 101/220 (45.91%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    S    +  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      ++ +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L  N         + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKAAKDPSRVGDAQNPLLSGGDLTTMIGKGTGAASFDEFGNNKYQNRRTMSSSDRAMMNAFKEITNMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKALETNVDLITTGDFMSRFCSNLGLTK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 51.9878 bits (123), Expect = 3.481e-22
Identity = 22/35 (62.86%), Postives = 26/35 (74.29%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L K+ C  H  A+L+EDY AGD+ICS C LVVGDR
Sbjct:   10 LPKVTCPNHPDALLVEDYRAGDMICSECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Mouse
Match: Gtf2b (general transcription factor IIB [Source:MGI Symbol;Acc:MGI:2385191])

HSP 1 Score: 69.707 bits (169), Expect = 7.230e-21
Identity = 63/223 (28.25%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLCLPK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.447 bits (119), Expect = 7.230e-21
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Match: sp|P29052|TF2B_DROME (Transcription initiation factor IIB OS=Drosophila melanogaster OX=7227 GN=TfIIB PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.025e-22
Identity = 63/222 (28.38%), Postives = 106/222 (47.75%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYM 882
            RVID  +EWR+F+N+  G D  RVG  E    S    S  I     +      G  K++N   +S +++ + +  + IS++ADR+ L   I   A  + K++        ++ +A ++ACL+  C++    R  KE  AV+ + K   I +  K+ +K L     S ++I     + RF   L + N+ V++    IAK A +      + P S+ A  IYM
Sbjct:   43 RVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKE-IGRCFKLTLKAL---ETSVDLITTADFMCRFCANLDLPNM-VQRAATHIAKKAVEMDIVPGRSPISVAAAAIYM 259

HSP 2 Score: 51.2174 bits (121), Expect = 2.025e-22
Identity = 22/34 (64.71%), Postives = 26/34 (76.47%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +K+CC  H  + LIEDY AGD+ICS C LVVGDR
Sbjct:   10 NKVCCYAHPESPLIEDYRAGDMICSECGLVVGDR 43

HSP 3 Score: 27.335 bits (59), Expect = 2.025e-22
Identity = 11/34 (32.35%), Postives = 20/34 (58.82%), Query Frame = 2

Query:  905 R*QENIKNVVGVSENTTASVYKILFENKESLIPE 1006
            R Q+ I ++ GV++ T    YK+++ +   L PE
Sbjct:  268 RSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPE 301

[Close]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Match: sp|P29054|TF2B_XENLA (Transcription initiation factor IIB OS=Xenopus laevis OX=8355 GN=gtf2b PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.192e-20
Identity = 58/220 (26.36%), Postives = 101/220 (45.91%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    S    +  I +   +    E G  K++N   +S +++ +    + I+ ++DR+ L   I      + K++ +      ++ +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L  N         + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKAAADPSRVGDAQNPLLSGGDLTTMIGKGTGSASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITNMSDRINLPRNIIDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKALETNVDLITTGDFMSRFCSNLGLTK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 51.9878 bits (123), Expect = 1.192e-20
Identity = 22/35 (62.86%), Postives = 26/35 (74.29%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L K+ C  H  A+L+EDY AGD+ICS C LVVGDR
Sbjct:   10 LPKVTCPNHPDALLVEDYRAGDMICSECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Match: sp|P62916|TF2B_RAT (Transcription initiation factor IIB OS=Rattus norvegicus OX=10116 GN=Gtf2b PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.294e-20
Identity = 63/223 (28.25%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLCLPK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.447 bits (119), Expect = 4.294e-20
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Match: sp|P62915|TF2B_MOUSE (Transcription initiation factor IIB OS=Mus musculus OX=10090 GN=Gtf2b PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.294e-20
Identity = 63/223 (28.25%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLCLPK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.447 bits (119), Expect = 4.294e-20
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5R886|TF2B_PONAB (Transcription initiation factor IIB OS=Pongo abelii OX=9601 GN=GTF2B PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.662e-20
Identity = 63/223 (28.25%), Postives = 104/223 (46.64%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYMG 885
            RVID  +EWR+F+ND    D  RVG  +    SD   S  I +   A    E G  K++N   +S +++ +    + I+ +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM 
Sbjct:   44 RVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLCLPK-QVQMAATHIARKAVELDLVPGRSPISVAAAAIYMA 261

HSP 2 Score: 50.447 bits (119), Expect = 4.662e-20
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Match: A0A1W0X8M1 (Transcription initiation factor IIB OS=Hypsibius dujardini OX=232323 GN=BV898_02234 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.200e-25
Identity = 62/222 (27.93%), Postives = 108/222 (48.65%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGG-DDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF---VFKKPSSIMAGTIYMGCL 891
            RVID   EWR+F+N+ G DD  RVG  E    +    S  I  +  +  L   G  K+++   +S A++ + T    IS +A+R+ +   I   A  + K++ +      ++ EAI++ACLF  C++    R  KE  A++ + K   I +  K+I++ L  +         + RF   L +   S+++V   IA+ A  F     + P S+ A  I++ C+
Sbjct:   42 RVIDVGTEWRTFSNESGTDDPSRVGGPENPLLNGQELSTMIGSATGSAGLDAQGFNKYRSRKTVSSADRALTTAFREISTMAERINMARTIVDQANTLFKQVYETKALRGRSTEAIASACLFIACRKEGVPRTFKEICAISRVPKKE-IGRCFKLILRALETSVDLVTTGDYMARFCANLSLSQ-SIQRVASTIARRASDFDVVPGRSPISVAAAAIFLACV 261

HSP 2 Score: 51.9878 bits (123), Expect = 2.200e-25
Identity = 20/34 (58.82%), Postives = 26/34 (76.47%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +++CC  H  A+L+EDY AGDL+C  C LVVGDR
Sbjct:    9 ARVCCPSHPEAILVEDYRAGDLVCPQCGLVVGDR 42

HSP 3 Score: 29.6462 bits (65), Expect = 2.200e-25
Identity = 13/31 (41.94%), Postives = 18/31 (58.06%), Query Frame = 2

Query:  914 ENIKNVVGVSENTTASVYKILFENKESLIPE 1006
            + I  + GV+E+T  +VYK L     SL PE
Sbjct:  270 KEIGEIAGVAESTIRAVYKFLHPKAASLFPE 300

[Close]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Match: A0A1I8GW91 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.189e-24
Identity = 69/235 (29.36%), Postives = 115/235 (48.94%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD-DNCRVGXXXX----XXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTA---GKFVFKKPSSIMAGTIYMGCLLMDIAMTRK 918
            RVID  +EWR+FANDG + D  RVG+ E     S       + T ++S+      E G   +KN   LS  ++ + +  + I  +A+RL L   +  +A K+ K++        +  E I++ACL+  C++ +  R +KE  +V+   + + I K  + I K L  N K  +    + RF   L++ N++++K    IA+ A    +   K P S+ A  IYM C   D    +K
Sbjct:  144 RVIDVGSEWRTFANDGSNKDMSRVGSAENPLLESTELTTRIANTGRESYGVD--PETGRPVYKNKKNLSIGDRTLTSAFKEIDQMANRLNLPKSVVNTANKIFKQVYDSKQCRGRANETIASACLYMACRQEAVPRTLKEVCSVSHSNRRD-IGKAFRKITKALDTNLKGISTTDFMSRFCSNLEL-NIAIQKAATHIAEKAMQIDEVAGKSPISVAAAAIYMACQASDTKKEKK 374

HSP 2 Score: 56.225 bits (134), Expect = 1.189e-24
Identity = 23/33 (69.70%), Postives = 26/33 (78.79%), Query Frame = 2

Query:   92 KLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            K+ CK+H  AVLIEDY  GDL+CS C LVVGDR
Sbjct:  112 KVTCKYHPEAVLIEDYHCGDLVCSQCGLVVGDR 144

[Close]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Match: A0A1I8GT66 (TFIIB-type domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.428e-23
Identity = 69/235 (29.36%), Postives = 115/235 (48.94%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD-DNCRVGXXX----XXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTA---GKFVFKKPSSIMAGTIYMGCLLMDIAMTRK 918
            RVID  +EWR+FANDG + D  RVG+ E     S       + T ++S+      E G   +KN   LS  ++ + +  + I  +A+RL L   +  +A K+ K++        +  E I++ACL+  C++ +  R +KE  +V+   + + I K  + I K L  N K  +    + RF   L++ N++++K    IA+ A    +   K P S+ A  IYM C   D    +K
Sbjct:  185 RVIDVGSEWRTFANDGSNKDMSRVGSAENPLLESTELTTRIANTGRESYGVD--PETGRPVYKNKKNLSIGDRTLTSAFKEIDQMANRLNLPKSVVNTANKIFKQVYDSKQCRGRANETIASACLYMACRQEAVPRTLKEVCSVSHSNRRD-IGKAFRKITKALDTNLKGISTTDFMSRFCSNLEL-NIAIQKAATHIAEKAMQIDEVAGKSPISVAAAAIYMACQASDTKKEKK 415

HSP 2 Score: 53.5286 bits (127), Expect = 7.428e-23
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 2

Query:   77 YFFLSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +  + K+ CK+H  AVLIEDY  GDL+CS C LVVGDR
Sbjct:  148 FRVVHKVTCKYHPEAVLIEDYHCGDLVCSQCGLVVGDR 185

[Close]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Match: A0A1I8I2U5 (TFIIB-type domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.421e-23
Identity = 69/235 (29.36%), Postives = 115/235 (48.94%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD-DNCRVGXXX----XXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTA---GKFVFKKPSSIMAGTIYMGCLLMDIAMTRK 918
            RVID  +EWR+FANDG + D  RVG+ E     S       + T ++S+      E G   +KN   LS  ++ + +  + I  +A+RL L   +  +A K+ K++        +  E I++ACL+  C++ +  R +KE  +V+   + + I K  + I K L  N K  +    + RF   L++ N++++K    IA+ A    +   K P S+ A  IYM C   D    +K
Sbjct:  144 RVIDVGSEWRTFANDGSNKDMSRVGSAENPLLESTELTTRIANTGRESYGVD--PETGRPVYKNKKNLSIGDRTLTSAFKEIDQMANRLNLPKSVVNTANKIFKQVYDSKQCRGRANETIASACLYMACRQEAVPRTLKEVCSVSHSNRRD-IGKAFRKITKALDTNLKGISTTDFMSRFCSNLEL-NIAIQKAATHIAEKAMQIDEVAGKSPISVAAAAIYMACQASDTKKEKK 374

HSP 2 Score: 53.5286 bits (127), Expect = 8.421e-23
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 2

Query:   77 YFFLSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +  + K+ CK+H  AVLIEDY  GDL+CS C LVVGDR
Sbjct:  107 FRVVHKVTCKYHPEAVLIEDYHCGDLVCSQCGLVVGDR 144

[Close]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Match: A0A151WLM7 (Transcription initiation factor IIB OS=Trachymyrmex zeteki OX=64791 GN=ALC60_12270 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.283e-22
Identity = 64/222 (28.83%), Postives = 105/222 (47.30%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYM 882
            RVID  +EWR+F+N+  G D  RVG  E    + A  S  I     A      G  K++N   +S +++ +      I+ +ADR+ L   I   A  + K++        +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L + N+ V++    IA+ A +      + P S+ A  IYM
Sbjct:   55 RVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLNGADLSTMIGPGTGAASFDGFGTAKYQNRRTMSSSDRTLLNAFREINGMADRINLPKTIVDRANTLFKQVHDGKNLKGRANDAIASACLYIACRQEGVPRTFKEICAVSKISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLGLPNM-VQRAATHIARKAVEIDIVPGRSPISVAAAAIYM 271

HSP 2 Score: 56.6102 bits (135), Expect = 2.283e-22
Identity = 26/43 (60.47%), Postives = 29/43 (67.44%), Query Frame = 2

Query:   62 YKFIYYFFLSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +KF Y    +K+CC  H  A LIEDY AGD ICS C LVVGDR
Sbjct:   13 FKFSYRNATNKVCCYAHPDAPLIEDYRAGDQICSECGLVVGDR 55

HSP 3 Score: 26.1794 bits (56), Expect = 2.283e-22
Identity = 11/34 (32.35%), Postives = 19/34 (55.88%), Query Frame = 2

Query:  905 R*QENIKNVVGVSENTTASVYKILFENKESLIPE 1006
            R Q+ I +  GV++ T    YK+++ +   L PE
Sbjct:  280 RSQKEIGDTAGVADVTIRQSYKLMYPHASQLFPE 313

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Cavefish
Match: gtf2b (general transcription factor IIB [Source:NCBI gene;Acc:103039385])

HSP 1 Score: 59.6918 bits (143), Expect = 5.566e-18
Identity = 37/140 (26.43%), Postives = 67/140 (47.86%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAV 654
            RVID  +EWR+F N+    D  RVG  +    +    +  I +   A    E G  K++N   +S +++ +    + IS +ADR+ L   I      + K++ +      ++ +AI++ACL+  C++    R  KE   +
Sbjct:   44 RVIDVGSEWRTFTNEKATKDPSRVGDAQNPLLNGGDLTTMISKGTGAASFDEFGNSKYQNRRTMSSSDRAMLNAFKEISTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEIGDI 183

HSP 2 Score: 50.447 bits (119), Expect = 5.566e-18
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   10 LPRVQCPNHPEAILVEDYRAGDMICPECGLVVGDR 44

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Yeast
Match: SUA7 (Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II [Source:SGD;Acc:S000006290])

HSP 1 Score: 48.9062 bits (115), Expect = 2.776e-11
Identity = 63/246 (25.61%), Postives = 97/246 (39.43%), Query Frame = 1

Query:  238 RVIDFSNEWRSFAND--GGDDNCRVGXXXXXXXXXXXXXXX--XKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNI-----------KEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVR---NVSVE---KVCIEIAKTAGKFVFKKPSSIMAGTIYMGCLLMDIAMT 912
            +++D  +EWR+F+ND   GDD  RVG            S      ++ D +   E  + + KN  V+   +  ++     I+ + D  +L  I+K  A +  K          K+ E+I AA +   C+     R             KEF     + K  L  K     +K+   N      +  IPRF   L +      S E   K C EI + AG    K P +I   +IY+  LL  I +T
Sbjct:   55 KLVDTRSEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETTDMRFTKELNKAQGKN--VMDKKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQVTTSAEYTAKKCKEIKEIAG----KSPITIAVVSIYLNILLFQIPIT 294

HSP 2 Score: 29.6462 bits (65), Expect = 2.776e-11
Identity = 11/30 (36.67%), Postives = 21/30 (70.00%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            CK +   + +E +S GD++C++C LV+ D+
Sbjct:   27 CKVYPPKI-VERFSEGDVVCALCGLVLSDK 55

HSP 3 Score: 25.0238 bits (53), Expect = 2.776e-11
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 2

Query:  938 VSENTTASVYKILFENKESLI 1000
            V+E T  S YKIL+E+++ L+
Sbjct:  304 VTEGTIKSGYKILYEHRDKLV 324

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Nematostella
Match: EDO36932 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SH79])

HSP 1 Score: 55.8398 bits (133), Expect = 4.221e-14
Identity = 58/217 (26.73%), Postives = 101/217 (46.54%), Query Frame = 1

Query:  256 NEWRSFAND-GGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKF---VFKKPSSIMAGTIYMG 885
            +EWR+F+N+    D  RVGA +    S    S  I++        E   +   +   +S A++ +      IS +ADR+ L   I   A ++ K++ +      ++ + I++ACL+  C++    R +KE  A+  + K   I +  K+I+K L     S ++I     + RF   L +  +SV+K    IA+ A +      + P SI A  IYM 
Sbjct:   44 SEWRTFSNEKATSDPSRVGAAQNPLLSGGDLSTLIEKPSGGNADIEGFARTHNSRSGMSGADRSLMNAFREISIMADRINLPRKIVDRANQLFKQVHEQKSLKGRSNDTIASACLYIACRQEGVPRTLKEICAIGKISKKE-IGRCFKLILKHL---ETSVDLITSGDFMSRFCSNLTL-PLSVQKAATYIARRAVELDLVAGRSPISIAAAAIYMA 255

HSP 2 Score: 39.6614 bits (91), Expect = 4.221e-14
Identity = 16/27 (59.26%), Postives = 19/27 (70.37%), Query Frame = 2

Query:   95 LCCKFHKTAVLIEDYSAGDLICSVCAL 175
            + C+ H  A LIEDY AGD+ICS C L
Sbjct:    7 VACRHHPDAPLIEDYRAGDMICSECGL 33

[Close]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Medaka
Match: gtf2b (general transcription factor IIB [Source:NCBI gene;Acc:101158724])

HSP 1 Score: 66.6254 bits (161), Expect = 5.365e-20
Identity = 59/222 (26.58%), Postives = 103/222 (46.40%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD-DNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQ---IPRFLDRLKVRNVSVEKVCIEIAKTAGKFVF---KKPSSIMAGTIYM 882
            RVID  +EWR+F+N+    D  RVG  +    +    +  I +   +    E G  K++N   +S +++ +    + IS +ADR+ L   I      + K++ +      +  +AI++ACL+  C++    R  KE  AV+ + K   I +  K+I+K L     S ++I     + RF   L +    V+     IA+ A +      + P S+ A  IYM
Sbjct:   46 RVIDVGSEWRTFSNEKAQKDPSRVGDAQNPLLNGGDLTTMIGKGTGSASFDEFGNSKYQNRRTMSSSDRAMLHAFKEISTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE-IGRCFKLILKAL---ETSVDLITTGDFMSRFCSNLGLPK-QVQMAATFIARKAVELDLVPGRSPISVAAAAIYM 262

HSP 2 Score: 50.447 bits (119), Expect = 5.365e-20
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 2

Query:   86 LSKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            L ++ C  H  A+L+EDY AGD+IC  C LVVGDR
Sbjct:   12 LPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR 46

[Close]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Match: SMESG000077013.1 (SMESG000077013.1)

HSP 1 Score: 161.77 bits (408), Expect = 1.674e-52
Identity = 102/231 (44.16%), Postives = 141/231 (61.04%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDG-GDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGK---FVFKKPSSIMAGTIYMGCLLMDIAMTRK 918
            RVID S+EWRSFANDG G+D+ RVGA+E +  S+A TSI IK S D +HL ENG QK+KN+V +SP E+M++ C   I+   +RL   S  +  AM +VKKL + + F RKNK AIS ACLF   ++NS  R  KE  AV+ + K + I K  K ++  +G+     ++ V + RF  RL  +N  V  +  EIA  A        K P++I+A +IYM  LLM+   +RK
Sbjct:   37 RVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKD-IGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKIISSKSPTTIIATSIYMASLLMNEERSRK 266

HSP 2 Score: 53.9138 bits (128), Expect = 1.674e-52
Identity = 22/34 (64.71%), Postives = 28/34 (82.35%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +++ CK+H  A LIED++AGDLIC VC LVVGDR
Sbjct:    4 NQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDR 37

HSP 3 Score: 30.8018 bits (68), Expect = 1.674e-52
Identity = 12/28 (42.86%), Postives = 20/28 (71.43%), Query Frame = 2

Query:  920 IKNVVGVSENTTASVYKILFENKESLIP 1003
            I +++GVS+ T A+ YKI + N+  L+P
Sbjct:  268 IGDILGVSQGTIATTYKIFYLNRHVLVP 295

[Close]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Match: SMESG000077016.1 (SMESG000077016.1)

HSP 1 Score: 111.694 bits (278), Expect = 1.482e-33
Identity = 80/225 (35.56%), Postives = 125/225 (55.56%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTA---GKFVFKKPSSIMAGTIYMGCLLMDI 903
            RVIDF +EWRSFAND   D  RVG +ET   S+  TSITI+QS + +HL E+G Q +K+   LS +++ ++T +  I A+ DR+ L   +   AM + K++ +      +N EA++ +CLF  C++    R  KE  AV+ + K + I K+ K I ++L  +  +      + RF  +L +    +E++  EI+K A        + P SI A +IYM  L M I
Sbjct:   37 RVIDFESEWRSFANDDAQDMSRVGEVETGLKSETETSITIQQSSNIRHLDESGNQIYKSRSTLSSSDRALKTAHSNIRAMGDRINLPKNLTDLAMGLFKQVHETKQLRGRNNEAVATSCLFMACRQLKVPRTFKEICAVSNVSKKD-IGKIFKKIGRILSTSVGNATGEDFMARFCYQLGIPR--LERIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGI 258

HSP 2 Score: 50.447 bits (119), Expect = 1.482e-33
Identity = 20/34 (58.82%), Postives = 28/34 (82.35%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +K+ CK+H TA LIED++AGDL+C+ C LVV +R
Sbjct:    4 NKVHCKYHPTATLIEDHTAGDLVCTRCGLVVAER 37

[Close]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Match: SMESG000019400.1 (SMESG000019400.1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.128e-19
Identity = 62/202 (30.69%), Postives = 103/202 (50.99%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD--DNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF 837
            R ID S+EWR+FA+DG D  D CRVGA+ET  T    ++   +  F+ + L ENG  KFK     +  E  + +    I+ + +R+K+   +   ++ ++ ++TK++   ++NK A++  CL+  C EN   R +KE      L       K+ K++   L   ++  N I+ I RFL  L V +  +E +  E  K   K 
Sbjct:   33 RAIDPSSEWRTFASDGADGHDACRVGAVETEETDGQTSTTV-QTGFNLRFLDENGNSKFKYLSSSTNKEVSLASQKRNIADLCERIKVDRNVYSRSVLLLHEITKLNVVGKRNKVALNCVCLYLACLENCVPRLLKEILNECPLVDEKKFKKLLKLVQITLKRPTQEINPILLIARFLYYLDVTDFKIENLVKEAVKNVSKI 233

HSP 2 Score: 38.891 bits (89), Expect = 1.128e-19
Identity = 16/30 (53.33%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            CK+H   VLI D   GD++CS C L+V DR
Sbjct:    4 CKYHPYKVLITDIRTGDIVCSACGLIVVDR 33

[Close]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Match: SMESG000019348.1 (SMESG000019348.1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.360e-19
Identity = 59/202 (29.21%), Postives = 102/202 (50.50%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGD--DNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTENGEQKFKNYVVLSPAEKMIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAACLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNIIVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKF 837
            R ID S+EWR+FA+DG D  D CRVGA+ET  T    ++   +  F+ + L ENG  KFK     +  E  + +    I+ + +R+K+   +   ++ ++ ++ K++  +++NK  ++  CL+  C +N   R +KE      L       K+ K++   L   ++  N I+ I RFL  L V +  +E +  E  K   K 
Sbjct:   35 RAIDPSSEWRTFASDGADGHDACRVGAVETEETDGQTSTTV-QTGFNLRFLDENGNSKFKYLSSSTNKEVSLASQKRNIADLCERIKVDRNVYSRSVFLLHEIIKLNAVSKRNKATLNCVCLYLACLDNGVPRLLKEIINECPLVDEKKFKKLLKLVQITLKRPTQEINPILLIARFLYYLDVTDFKIENLVKEAVKNVSKI 235

HSP 2 Score: 39.2762 bits (90), Expect = 2.360e-19
Identity = 16/30 (53.33%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  101 CKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            CK+H   VLI D   GD++CS C L+V DR
Sbjct:    6 CKYHPYKVLITDIRTGDIVCSACGLIVVDR 35

[Close]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Match: SMESG000077015.1 (SMESG000077015.1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.734e-13
Identity = 20/34 (58.82%), Postives = 29/34 (85.29%), Query Frame = 2

Query:   89 SKLCCKFHKTAVLIEDYSAGDLICSVCALVVGDR 190
            +K+ CK+H TA LIED++AGDL+C++C LVV +R
Sbjct:    4 NKVHCKYHPTATLIEDHTAGDLVCTLCGLVVAER 37

HSP 2 Score: 43.1282 bits (100), Expect = 1.734e-13
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = 1

Query:  238 RVIDFSNEWRSFANDGGDDNCRVGXXXXXXXXXXXXXXXXKQSFDAKHLTEN 393
            RVID+ +EWRSFAND   + CRVG +E++  S+  TSI ++QS + + L E+
Sbjct:   37 RVIDYESEWRSFANDDNQEMCRVGEMESAFRSETETSIMMQQSSNIRLLDES 88

The following BLAST results are available for this feature:

BLAST of TFIIB-type domain-containing protein vs. Ensembl Human
BLAST of TFIIB-type domain-containing protein vs. Ensembl Celegans
BLAST of TFIIB-type domain-containing protein vs. Ensembl Fly
BLAST of TFIIB-type domain-containing protein vs. Ensembl Zebrafish
BLAST of TFIIB-type domain-containing protein vs. Ensembl Xenopus
BLAST of TFIIB-type domain-containing protein vs. Ensembl Mouse
BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
BLAST of TFIIB-type domain-containing protein vs. TrEMBL
BLAST of TFIIB-type domain-containing protein vs. Ensembl Cavefish
BLAST of TFIIB-type domain-containing protein vs. Ensembl Sea Lamprey
BLAST of TFIIB-type domain-containing protein vs. Ensembl Yeast
BLAST of TFIIB-type domain-containing protein vs. Ensembl Nematostella
BLAST of TFIIB-type domain-containing protein vs. Ensembl Medaka
BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST

BLAST of TFIIB-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
GTF2B	1.075e-20	28.25	general transcription factor IIB [Source:HGNC Symb...	[more]
GTF2B	4.019e-19	30.00	general transcription factor IIB [Source:HGNC Symb...	[more]
GTF2B	2.126e-16	30.00	general transcription factor IIB [Source:HGNC Symb...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ttb-1	4.221e-20	30.94	Transcription initiation factor IIB [Source:UniPr...	[more]
ttb-1	4.221e-20	30.94	Transcription initiation factor IIB [Source:UniPr...	[more]
ttb-1	4.221e-20	30.94	Transcription initiation factor IIB [Source:UniPr...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
TfIIB	2.675e-23	28.38	gene:FBgn0004915 transcript:FBtr0080025	[more]
TfIIB	2.675e-23	28.38	gene:FBgn0004915 transcript:FBtr0333489	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gtf2b	2.576e-20	26.46	general transcription factor IIB [Source:ZFIN;Acc:...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rars2	3.423e-22	27.27	arginyl-tRNA synthetase 2, mitochondrial [Source:X...	[more]
rars2	3.481e-22	27.27	arginyl-tRNA synthetase 2, mitochondrial [Source:X...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Gtf2b	7.230e-21	28.25	general transcription factor IIB [Source:MGI Symbo...	[more]

BLAST of TFIIB-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P29052\|TF2B_DROME	2.025e-22	28.38	Transcription initiation factor IIB OS=Drosophila ...	[more]
sp\|P29054\|TF2B_XENLA	1.192e-20	26.36	Transcription initiation factor IIB OS=Xenopus lae...	[more]
sp\|P62916\|TF2B_RAT	4.294e-20	28.25	Transcription initiation factor IIB OS=Rattus norv...	[more]
sp\|P62915\|TF2B_MOUSE	4.294e-20	28.25	Transcription initiation factor IIB OS=Mus musculu...	[more]
sp\|Q5R886\|TF2B_PONAB	4.662e-20	28.25	Transcription initiation factor IIB OS=Pongo abeli...	[more]

BLAST of TFIIB-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1W0X8M1	2.200e-25	27.93	Transcription initiation factor IIB OS=Hypsibius d...	[more]
A0A1I8GW91	1.189e-24	29.36	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8GT66	7.428e-23	29.36	TFIIB-type domain-containing protein OS=Macrostomu...	[more]
A0A1I8I2U5	8.421e-23	29.36	TFIIB-type domain-containing protein OS=Macrostomu...	[more]
A0A151WLM7	2.283e-22	28.83	Transcription initiation factor IIB OS=Trachymyrme...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gtf2b	5.566e-18	26.43	general transcription factor IIB [Source:NCBI gene...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of TFIIB-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
SUA7	2.776e-11	25.61	Transcription factor TFIIB; a general transcriptio...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO36932	4.221e-14	26.73	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of TFIIB-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gtf2b	5.365e-20	26.58	general transcription factor IIB [Source:NCBI gene...	[more]

BLAST of TFIIB-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000077013.1	1.674e-52	44.16	SMESG000077013.1	[more]
SMESG000077016.1	1.482e-33	35.56	SMESG000077016.1	[more]
SMESG000019400.1	1.128e-19	30.69	SMESG000019400.1	[more]
SMESG000019348.1	2.360e-19	29.21	SMESG000019348.1	[more]
SMESG000077015.1	1.734e-13	58.82	SMESG000077015.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30033223 ID=SMED30033223|Name=TFIIB-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1088bp
GATCTAAATGAAGTAAGTAGTAAATTTTAATATAAAGAATTTTTATTTTT
TATAAGATATATATAAATTTATTTACTACTTTTTTCTTAGTAAACTTTGT
TGTAAATTCCACAAAACAGCAGTTTTGATAGAAGACTATTCAGCGGGAGA
CTTGATCTGTTCTGTTTGTGCGTTAGTTGTTGGCGATCGGTAAGGATTAA
TTAAACAAATAATTAAAAATTAATTTAAAAAAAATGTAGAGTGATTGACT
TCTCCAATGAATGGAGGTCATTTGCTAATGATGGGGGCGATGACAACTGT
CGAGTTGGGGCCATAGAAACCAGTGCGACCTCTGATGCTGCTACATCTAT
TACGATAAAACAATCATTCGACGCCAAACATTTGACTGAAAATGGAGAAC
AGAAATTCAAAAACTACGTTGTCCTTTCACCGGCAGAAAAAATGATTAGA
ACGTGCAATGAGTTCATTTCAGCAATTGCTGATCGGTTGAAGCTCATATC
AATAATAAAAATTTCTGCAATGAAAATGGTAAAGAAATTGACTAAAATTG
ACGGATTCACTCGGAAAAATAAAGAAGCAATATCGGCAGCTTGTTTATTT
CATTGCTGCAAAGAAAATAGCTGTGAAAGAAATATAAAAGAATTCGCTGC
CGTTACCACTTTGGAAAAGCCCAATCTGATCTTGAAAATGAACAAGATAA
TCGTCAAAGTTCTCGGATTGAATTCGAAATCCACAAACATCATTGTTCAG
ATCCCTCGATTTTTGGATAGGTTGAAGGTGAGAAATGTCAGCGTAGAAAA
GGTTTGCATAGAAATCGCAAAGACAGCCGGTAAATTCGTGTTTAAGAAAC
CCTCGAGTATCATGGCAGGCACAATTTACATGGGCTGCTTACTTATGGAT
ATAGCGATGACAAGAAAATATTAAAAATGTCGTCGGAGTATCAGAGAACA
CGACAGCTTCAGTATATAAGATTCTCTTTGAAAATAAAGAATCGCTGATA
CCAGAATCCGTGTTGCCACTCTTAAAAGTACCCCTCAACAAGCTACGAAC
GGATTGACACTAAGAATTTCTTTTCAATTTATACGATC

protein sequence of SMED30033223-orf-1

>SMED30033223-orf-1 ID=SMED30033223-orf-1|Name=SMED30033223-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MIRTCNEFISAIADRLKLISIIKISAMKMVKKLTKIDGFTRKNKEAISAA
CLFHCCKENSCERNIKEFAAVTTLEKPNLILKMNKIIVKVLGLNSKSTNI
IVQIPRFLDRLKVRNVSVEKVCIEIAKTAGKFVFKKPSSIMAGTIYMGCL
LMDIAMTRKY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0046872	metal ion binding
GO:0008270	zinc ion binding
GO:0017025	TBP-class protein binding
GO:0003743	translation initiation factor activity

Vocabulary: **biological process**
Term	Definition
GO:0070897	DNA-templated transcriptional preinitiation complex assembly
GO:0006352	DNA-templated transcription, initiation
GO:0006355	regulation of transcription, DNA-templated
GO:0006413	translational initiation

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000074	oocyte
PLANA:0000231	vitelline gland

Vocabulary: **INTERPRO**
Term	Definition
IPR036915	Cyclin-like_sf
IPR013137	Znf_TFIIB
IPR000812	TFIIB
IPR013763	Cyclin-like
IPR013150	TFIIB_cyclin

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000812	Transcription factor TFIIB	PRINTS	PR00685	TIFACTORIIB	coord: 135..151 score: 36.47 coord: 49..68 score: 32.0
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 5..160
None	No IPR available	GENE3D	G3DSA:1.10.472.10		coord: 3..96 e-value: 6.4E-12 score: 47.4
IPR013150	Transcription factor TFIIB, cyclin-like domain	PFAM	PF00382	TFIIB	coord: 9..75 e-value: 2.0E-6 score: 27.7
IPR013763	Cyclin-like	CDD	cd00043	CYCLIN	coord: 1..88 e-value: 9.30154E-4 score: 34.5217
IPR036915	Cyclin-like superfamily	SUPERFAMILY	SSF47954	Cyclin-like	coord: 101..159
IPR036915	Cyclin-like superfamily	SUPERFAMILY	SSF47954	Cyclin-like	coord: 5..94

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TFIIB-type domain-containing protein

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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