Long-chain-fatty-acid--CoA ligase ACSBG2
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033222 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Match: ACSBG1 (acyl-CoA synthetase bubblegum family member 1 [Source:HGNC Symbol;Acc:HGNC:29567]) HSP 1 Score: 430.254 bits (1105), Expect = 1.866e-148 Identity = 214/470 (45.53%), Postives = 307/470 (65.32%), Query Frame = 1
Query: 766 EFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
EFM LG + ++ L ++ PN+CC L+YTSGTTGNPKGVM+S DN+ WTA R + +E+++SYLPLSH+AAQ+ D+ + A + FA+PDALKGSL +TL+EV PT +GVPRV+EKIME+++E+A +++ + WA + NL + PF +A ++ KV +ALGF +C F AAP+ + +F L++ + YG+SE SGP + +R S GK++P KL ++D + GEIC++GR +FMGY+N +KT E D E WLH+GD G + +G+L+ITGR+KEL+ITAGGEN+ PVPIE+ +K L ISNAM++GD+RK+L +LLT+KC +DP T TD+LT A + +G T++S+II K+ + +I+EGI+R N NA +R +QKW L DFS+ GGELGPT+KLKR V E Y +I+S Y+
Sbjct: 251 EFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKLVNQDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTMKLKRLTVLEKYKGIIDSFYQ 720 HSP 2 Score: 117.857 bits (294), Expect = 1.866e-148 Identity = 62/162 (38.27%), Postives = 97/162 (59.88%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
E +++T D +LR++ P T+H MF+ A+DK + AL +K ++ + I+YSQYY L AAK +++GL SV I G NSPEWF + GT+FAGG+ +GIY T++ E ++I +C V +V ++ L+K + + K P+LKA+V Y
Sbjct: 79 EALWTTRADGRVRLRIDPS---CPQLPYTVHRMFYEALDKYGDLIALGFK--RQDKWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILKIWKQLPHLKAVVIY 235
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Match: ACSBG2 (acyl-CoA synthetase bubblegum family member 2 [Source:HGNC Symbol;Acc:HGNC:24174]) HSP 1 Score: 436.417 bits (1121), Expect = 4.562e-145 Identity = 217/472 (45.97%), Postives = 308/472 (65.25%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK----LDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I D L + N+C LIYTSGTTG PKGVM+SHDN+ W A + K E ++SYLPLSH+AAQM DI +P+ A +FAQ DALKG+L TLKEV PT FIGVP+++EKI E +K+ + +KK WA++IG++ N KM+ K N P Y +AK ++F+KV +LG D C S APL+ + +F SLD+ I E+YG+SE SGP TI QN +R S GKIL GCK ++DG GEIC++GRH+FMGY+ + +T E D E WLHSGD+G + G+L++TG IKE+LITAGGEN+ P+P+E +K+ + ISNAM+VGDK K+L +LLT+KC ++ ++ P D L A + G+G +++++I+ ++P + +I +GI N+ A++ + ++KW L DFS+ GGELGP +KLKR+ V + Y I+ +Y
Sbjct: 10 WDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILT--GCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 478
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Match: ACSBG2 (acyl-CoA synthetase bubblegum family member 2 [Source:HGNC Symbol;Acc:HGNC:24174]) HSP 1 Score: 435.261 bits (1118), Expect = 3.426e-144 Identity = 217/472 (45.97%), Postives = 308/472 (65.25%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK----LDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I D L + N+C LIYTSGTTG PKGVM+SHDN+ W A + K E ++SYLPLSH+AAQM DI +P+ A +FAQ DALKG+L TLKEV PT FIGVP+++EKI E +K+ + +KK WA++IG++ N KM+ K N P Y +AK ++F+KV +LG D C S APL+ + +F SLD+ I E+YG+SE SGP TI QN +R S GKIL GCK ++DG GEIC++GRH+FMGY+ + +T E D E WLHSGD+G + G+L++TG IKE+LITAGGEN+ P+P+E +K+ + ISNAM+VGDK K+L +LLT+KC ++ ++ P D L A + G+G +++++I+ ++P + +I +GI N+ A++ + ++KW L DFS+ GGELGP +KLKR+ V + Y I+ +Y
Sbjct: 197 WDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILT--GCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 665 HSP 2 Score: 98.5969 bits (244), Expect = 3.426e-144 Identity = 58/174 (33.33%), Postives = 95/174 (54.60%), Query Frame = 2
Query: 146 FEIKQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNC-PNLKAIVYY 664
E+ + T +++T D LR++ G P+T+ F ++++ AL K ++ L N++QYY+ C AAKSLI++GL V I G NS EWFIT G I AGG+ GIY TN+ E +++I + + + +V N++ L+K + + ++ LKAI+ Y
Sbjct: 14 LEVDMNKTEVTPRLWTTCRDGEVLLRLSKHGP-GHETPMTIPEFFRESVNRFGTYPALASKNGKKWEIL--NFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQY 184
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Match: ACSBG2 (acyl-CoA synthetase bubblegum family member 2 [Source:HGNC Symbol;Acc:HGNC:24174]) HSP 1 Score: 435.261 bits (1118), Expect = 3.426e-144 Identity = 217/472 (45.97%), Postives = 308/472 (65.25%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK----LDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I D L + N+C LIYTSGTTG PKGVM+SHDN+ W A + K E ++SYLPLSH+AAQM DI +P+ A +FAQ DALKG+L TLKEV PT FIGVP+++EKI E +K+ + +KK WA++IG++ N KM+ K N P Y +AK ++F+KV +LG D C S APL+ + +F SLD+ I E+YG+SE SGP TI QN +R S GKIL GCK ++DG GEIC++GRH+FMGY+ + +T E D E WLHSGD+G + G+L++TG IKE+LITAGGEN+ P+P+E +K+ + ISNAM+VGDK K+L +LLT+KC ++ ++ P D L A + G+G +++++I+ ++P + +I +GI N+ A++ + ++KW L DFS+ GGELGP +KLKR+ V + Y I+ +Y
Sbjct: 197 WDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILT--GCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 665 HSP 2 Score: 98.5969 bits (244), Expect = 3.426e-144 Identity = 58/174 (33.33%), Postives = 95/174 (54.60%), Query Frame = 2
Query: 146 FEIKQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNC-PNLKAIVYY 664
E+ + T +++T D LR++ G P+T+ F ++++ AL K ++ L N++QYY+ C AAKSLI++GL V I G NS EWFIT G I AGG+ GIY TN+ E +++I + + + +V N++ L+K + + ++ LKAI+ Y
Sbjct: 14 LEVDMNKTEVTPRLWTTCRDGEVLLRLSKHGP-GHETPMTIPEFFRESVNRFGTYPALASKNGKKWEIL--NFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQY 184
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Match: ACSBG2 (acyl-CoA synthetase bubblegum family member 2 [Source:HGNC Symbol;Acc:HGNC:24174]) HSP 1 Score: 436.032 bits (1120), Expect = 5.441e-143 Identity = 217/472 (45.97%), Postives = 308/472 (65.25%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK----LDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I D L + N+C LIYTSGTTG PKGVM+SHDN+ W A + K E ++SYLPLSH+AAQM DI +P+ A +FAQ DALKG+L TLKEV PT FIGVP+++EKI E +K+ + +KK WA++IG++ N KM+ K N P Y +AK ++F+KV +LG D C S APL+ + +F SLD+ I E+YG+SE SGP TI QN +R S GKIL GCK ++DG GEIC++GRH+FMGY+ + +T E D E WLHSGD+G + G+L++TG IKE+LITAGGEN+ P+P+E +K+ + ISNAM+VGDK K+L +LLT+KC ++ ++ P D L A + G+G +++++I+ ++P + +I +GI N+ A++ + ++KW L DFS+ GGELGP +KLKR+ V + Y I+ +Y
Sbjct: 147 WDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILT--GCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 615 HSP 2 Score: 92.0485 bits (227), Expect = 1.528e-18 Identity = 50/136 (36.76%), Postives = 80/136 (58.82%), Query Frame = 2
Query: 260 ITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNC-PNLKAIVYY 664
+T+ F ++++ AL K ++ L N++QYY+ C AAKSLI++GL V I G NS EWFIT G I AGG+ GIY TN+ E +++I + + + +V N++ L+K + + ++ LKAI+ Y
Sbjct: 1 MTIPEFFRESVNRFGTYPALASKNGKKWEIL--NFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQY 134
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Match: acs-13 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:V6CLI5]) HSP 1 Score: 152.14 bits (383), Expect = 4.483e-38 Identity = 131/468 (27.99%), Postives = 226/468 (48.29%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS----KEMLISYLPLSHVAAQMCDIIIPLLC---QATIHFAQPDALKGSLGHTLKEVHPTYFIG----VPRVFEKIMEQ-----MKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPA-AKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
P + ++ YTSGTTG PKGVM++H N++ CM + F+HS + +IS+LPL+H M + +I +C A + F + D L +KE+ PT + R+++K+M + +K+M DF+ IS A+D+ ++ +G F ++F K+ + G R + + +APLS +VL + A++ V+ E YG +EC T+ + + G VG ++P KL + GE+C+ G +F GY ++TKE D + WLH+GD+G + EG L I R K + + GE +AP IE I +++ + V G+ K C++ ++ P ++ L PA A + G + ++ + K +I +D+ + + + +V+ + FSV L PTLK KR + YS ++++Y
Sbjct: 247 PEDLATICYTSGTTGTPKGVMLTHANVIADGVCMDF---FKHSGIAATDSMISFLPLAH----MLERVIESVCFCVGAKVGFYRGDIR--VLAEDIKELKPTVVPVVPRVLNRLYDKVMSEVNKSTLKKMLFDFA-----ISYKARDMANFN-----IRNDGFF-----DNLVFKKIREGFG-GRVRLMITGSAPLSTNVLTFVRAAMGCVVVEGYGQTECVAACTVSMEGDSLAGHVGMVIPSCQIKLVDVPELNYYAKDQAGEVCVKGHIIFKGYYKNEQQTKETIDEDGWLHTGDIGRWTPEGTLKIVDRKKHIFKLSQGEYVAPEKIEN-IYVRSKYVAQSFVHGESLK-TCLI----AIVVPDAEV----LVPAMADQGIKG------TFEELCNNDAVKKAI-LDDMVAVGKKAGLFSFEQVRDISLSAEQFSVENDLLTPTLKSKRPKLKAHYSKQLDAMY 672
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Match: acs-13 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:V6CLI5]) HSP 1 Score: 152.14 bits (383), Expect = 5.648e-38 Identity = 131/468 (27.99%), Postives = 226/468 (48.29%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS----KEMLISYLPLSHVAAQMCDIIIPLLC---QATIHFAQPDALKGSLGHTLKEVHPTYFIG----VPRVFEKIMEQ-----MKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPA-AKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
P + ++ YTSGTTG PKGVM++H N++ CM + F+HS + +IS+LPL+H M + +I +C A + F + D L +KE+ PT + R+++K+M + +K+M DF+ IS A+D+ ++ +G F ++F K+ + G R + + +APLS +VL + A++ V+ E YG +EC T+ + + G VG ++P KL + GE+C+ G +F GY ++TKE D + WLH+GD+G + EG L I R K + + GE +AP IE I +++ + V G+ K C++ ++ P ++ L PA A + G + ++ + K +I +D+ + + + +V+ + FSV L PTLK KR + YS ++++Y
Sbjct: 290 PEDLATICYTSGTTGTPKGVMLTHANVIADGVCMDF---FKHSGIAATDSMISFLPLAH----MLERVIESVCFCVGAKVGFYRGDIR--VLAEDIKELKPTVVPVVPRVLNRLYDKVMSEVNKSTLKKMLFDFA-----ISYKARDMANFN-----IRNDGFF-----DNLVFKKIREGFG-GRVRLMITGSAPLSTNVLTFVRAAMGCVVVEGYGQTECVAACTVSMEGDSLAGHVGMVIPSCQIKLVDVPELNYYAKDQAGEVCVKGHIIFKGYYKNEQQTKETIDEDGWLHTGDIGRWTPEGTLKIVDRKKHIFKLSQGEYVAPEKIEN-IYVRSKYVAQSFVHGESLK-TCLI----AIVVPDAEV----LVPAMADQGIKG------TFEELCNNDAVKKAI-LDDMVAVGKKAGLFSFEQVRDISLSAEQFSVENDLLTPTLKSKRPKLKAHYSKQLDAMY 715
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Match: acs-13 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:V6CLI5]) HSP 1 Score: 151.754 bits (382), Expect = 7.381e-38 Identity = 132/470 (28.09%), Postives = 228/470 (48.51%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS----KEMLISYLPLSHVAAQMCDIIIPLLC---QATIHFAQPDALKGSLGHTLKEVHPTYFIG----VPRVFEKIMEQ-----MKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE---------GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPA-AKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
P + ++ YTSGTTG PKGVM++H N++ CM + F+HS + +IS+LPL+H M + +I +C A + F + D L +KE+ PT + R+++K+M + +K+M DF+ IS A+D+ ++ +G F ++F K+ + G R + + +APLS +VL + A++ V+ E YG +EC T+ + + G VG ++P C++ D E GE+C+ G +F GY ++TKE D + WLH+GD+G + EG L I R K + + GE +AP IE I +++ + V G+ K C++ ++ P ++ L PA A + G + ++ + K +I +D+ + + + +V+ + FSV L PTLK KR + YS ++++Y
Sbjct: 297 PEDLATICYTSGTTGTPKGVMLTHANVIADGVCMDF---FKHSGIAATDSMISFLPLAH----MLERVIESVCFCVGAKVGFYRGDIR--VLAEDIKELKPTVVPVVPRVLNRLYDKVMSEVNKSTLKKMLFDFA-----ISYKARDMANFN-----IRNDGFF-----DNLVFKKIREGFG-GRVRLMITGSAPLSTNVLTFVRAAMGCVVVEGYGQTECVAACTVSMEGDSLAGHVGMVIP--SCQIKLVDVPELNYYAKDQAGEVCVKGHIIFKGYYKNEQQTKETIDEDGWLHTGDIGRWTPEGTLKIVDRKKHIFKLSQGEYVAPEKIEN-IYVRSKYVAQSFVHGESLK-TCLI----AIVVPDAEV----LVPAMADQGIKG------TFEELCNNDAVKKAI-LDDMVAVGKKAGLFSFEQVRDISLSAEQFSVENDLLTPTLKSKRPKLKAHYSKQLDAMY 722
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Match: acs-5 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q9XWD1]) HSP 1 Score: 133.265 bits (334), Expect = 4.926e-32 Identity = 131/446 (29.37%), Postives = 205/446 (45.96%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM-KFRHS----KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGY-SIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVL-VYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLD--------SEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDK-RKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPK-LSISIDEGIKRSNE-NAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKR 2115
P++ + YTSGTTG PKGVM++H N+V L + F+ S ++ ISYLPLSH+ Q+ + +F +++G L +K + PT F VPR+ ++ + + + K + ++A + +L K G G +I ++FNK+ + +G L + + +AP+S VL +L I E YG +EC+ T + + G G P KL ++DGK GEI + G V GY PE+T E+FD E +L +GD+G G + I R K + A GE +AP IEQ + + V GD ++L ++ +P D+ K W G I +I D+ K +S I ++N+ N+I +V+K F+V G L PTLK KR
Sbjct: 250 PDDNYIICYTSGTTGTPKGVMLTHSNIVANISGFLKILFAFQPSMIDATQVHISYLPLSHMMEQLTHWTLLGFGSKIGYFR--GSIQG-LTDDIKTLKPTVFPVVPRLLNRLYDAITSKVQQQGFMAKLVYNFA--FARKLSLVKA----GKVGRDTIWDRLVFNKIQQQIGGKVDL-MVTGSAPISSTVLETCRVTLGTTIVEGYGQTECTALATFTWMGDPSTGHCGAPAPCALVKLGDVPDLNYFAKDGK-GEIRIKGPCVTKGYYKDPERTAELFDEEGFLQTGDIGEMLPNGTIRIIDRKKHIFKLAQGEYVAPEKIEQ-VYIRTPVVQQVYVDGDSLERWLIAVV-----------VPEPDV---LKEWNDKQGSGSRKIEEICNDEKAKEFVLSELHAIGKANKLNSIE---QVKKVILTSDTFTVENGLLTPTLKAKR 666
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Match: acs-18 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q21872]) HSP 1 Score: 128.257 bits (321), Expect = 2.324e-30 Identity = 123/458 (26.86%), Postives = 199/458 (43.45%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKC----VIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKIL----------PDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
P ++ +TSGTTG PKGVM++H N+ + ++ +SYLPL+H+ ++C ++ + + I F++ D L ++ + P F VPRV +KI + + + D +KK I + A I Y+ KM G + K + +K+ LG + I AA + + + + E YG +E SGP T++ + R G VG + P+ G +D G E+ + G +V GY PE T F + ++ +GD+G F EG L I R K + G+ +AP + + + + F+ V GD K V + V DP + + LT K +G Y I+ D + + + E KR R V P FS G PTLK KRN + + + A I+ +Y+
Sbjct: 260 PETLATISFTSGTTGRPKGVMLTHLNMCSATMSCEEFENEAGVQDAYLSYLPLAHIYERLC-LLSNFMIGSRIGFSRGDP--KLLVDDVQALAPRSFATVPRVIDKIHKAVMKQVQD-KPLKKMILNAA--IAYKLYHYKMT------GKATRKTWVDKYVLHKIQMLLGPNIRQLILGAAKSDVSAMRFARGAFGVEVLEGYGQTETSGPTTLQLVGDTRIGCVGPPMACAMIKLIDVPELGYSVDKNGG---EVLVKGHNVTSGYYKNPEATASSFTEDGYMKTGDIGRFTAEGSLQIIDRRKNVFKMPQGKFVAP-DLTESLYTSSSFVQQIYVHGDMEKPWLVAI---VVPDP-EYLASYALT---KHNINGKTYEQLCNIPILADDVLRQFVELTEEDKR------PRYEGVYGVHLTPVAFSAENGLTTPTLKNKRNAIAQFFKAEIDGMYK 688
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Match: bgm (gene:FBgn0027348 transcript:FBtr0080590) HSP 1 Score: 432.95 bits (1112), Expect = 2.134e-141 Identity = 221/470 (47.02%), Postives = 310/470 (65.96%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM-KFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKN-PKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W+E ++ ++ +D + RL ++ NECC L+YTSGT G PKGVM+SHDN+ + ++ M + E ++SYLPLSHVAAQ DI I FA DALKG+L +L++ PT F+GVPRV+EK E+M +A S+KK ++SWAK I + + K +G F Y IAK +I +KV +ALGFDR L + SAAAP+S + YF SLDL I + +GMSE +G TI + ++GK LP K ++D GE+C+ GRHVFMGY++ EKT+E D + WLHSGD+G +D+GY+ +TGR KE++ITAGGENI PV IE IK+ LD ISNA +VG++RKYL VL+T+K +D + P D+L+ + W +G ++S I+ PK+ SI++ IKR+N+ +IS + +VQK+T LPHDFS+P GELGPTLK+KRNVV+++Y+ I LY
Sbjct: 196 WSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESKFINKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYADEIEKLY 665 HSP 2 Score: 76.2554 bits (186), Expect = 6.726e-14 Identity = 41/156 (26.28%), Postives = 79/156 (50.64%), Query Frame = 2
Query: 191 STSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIV 658
+T+ K+RM K + +PI++ + ++ + AL+ K + ++ + ++ Y QY Q AK+ I++GL SV + N EWF + G I A G+ +GIY TN+ + V+ ++ + + +V + + + K + P LKA +
Sbjct: 24 TTNRQDAVKIRM-AKDGIGAEEPISVPGLLKRTVNNYGDYPALRTK-NGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAI 177
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Match: bgm (gene:FBgn0027348 transcript:FBtr0346624) HSP 1 Score: 432.95 bits (1112), Expect = 2.134e-141 Identity = 221/470 (47.02%), Postives = 310/470 (65.96%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM-KFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKN-PKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W+E ++ ++ +D + RL ++ NECC L+YTSGT G PKGVM+SHDN+ + ++ M + E ++SYLPLSHVAAQ DI I FA DALKG+L +L++ PT F+GVPRV+EK E+M +A S+KK ++SWAK I + + K +G F Y IAK +I +KV +ALGFDR L + SAAAP+S + YF SLDL I + +GMSE +G TI + ++GK LP K ++D GE+C+ GRHVFMGY++ EKT+E D + WLHSGD+G +D+GY+ +TGR KE++ITAGGENI PV IE IK+ LD ISNA +VG++RKYL VL+T+K +D + P D+L+ + W +G ++S I+ PK+ SI++ IKR+N+ +IS + +VQK+T LPHDFS+P GELGPTLK+KRNVV+++Y+ I LY
Sbjct: 196 WSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESKFINKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYADEIEKLY 665 HSP 2 Score: 76.2554 bits (186), Expect = 6.726e-14 Identity = 41/156 (26.28%), Postives = 79/156 (50.64%), Query Frame = 2
Query: 191 STSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIV 658
+T+ K+RM K + +PI++ + ++ + AL+ K + ++ + ++ Y QY Q AK+ I++GL SV + N EWF + G I A G+ +GIY TN+ + V+ ++ + + +V + + + K + P LKA +
Sbjct: 24 TTNRQDAVKIRM-AKDGIGAEEPISVPGLLKRTVNNYGDYPALRTK-NGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAI 177
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Match: hll (gene:FBgn0286723 transcript:FBtr0080591) HSP 1 Score: 333.954 bits (855), Expect = 2.532e-103 Identity = 194/485 (40.00%), Postives = 275/485 (56.70%), Query Frame = 1
Query: 799 DTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK-FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQM--KEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAK----CVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQT--IEFQNEFRFGSVGKILPDFGCKLDSED-GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIIT--------------------DKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
+ L+ R + + NEC LI+TSGT G PK VM+SHDNLV+ + +M+ + KE +SYLPLSHVAAQ+ D+ + L + FA DALKG+L T ++ PT GVPRVFEK+ E++ E S + A ++T L M P Y AK C + + + +G D C F+ AP S ++ +F LD+ + E YGMSE SG T ++ N + S G+ K+ D +GEI M GR VFMGY+ P+KT+E + WLHSGD+G + +G L I+GR+KEL+ITAGGENI PV IE+ IK+ L +SN +++GD RKYL VLL++K D T IP D L W + + T +S+++ PKL +I+EGIKR+N+ AIS + +VQK+ + H+FSV GELGPTLK++RN+V+ Y+ +I LY+
Sbjct: 203 EELLARESRIRANECAMLIFTSGTVGMPKAVMLSHDNLVFDTKSAAAHMQDIQVGKESFVSYLPLSHVAAQIFDVFLGLSHAGCVTFADKDALKGTLIKTFRKARPTKMFGVPRVFEKLQERLVAAEAKARPYSRLLLARARAAVAEHQTTL---MAGKSPSIYGNAKYWLACRVVKPIREMIGVDNCRVFFTGGAPTSEELKQFFLGLDIALGECYGMSETSGAITLNVDISNLY---SAGQACEGVTLKIHEPDCNGQGEILMRGRLVFMGYLGLPDKTEETVKEDGWLHSGDLGYIDPKGNLIISGRLKELIITAGGENIPPVHIEELIKKELPCVSNVLLIGDHRKYLTVLLSLKTKCDAKTGIPLDALREETIEWLRDLDIHETRLSELLNIPADLQLPNDTAALAATLEITAKPKLLEAIEEGIKRANKYAISNAQKVQKFALIAHEFSVATGELGPTLKIRRNIVHAKYAKVIERLYK 681 HSP 2 Score: 87.4261 bits (215), Expect = 2.083e-17 Identity = 47/163 (28.83%), Postives = 83/163 (50.92%), Query Frame = 2
Query: 191 STSLDKVSKLRMNCKGDLAR-IKPITLHTMFHNAIDKGANTEALKYKY--SQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
STSL KLR++ ++ + P T+ F + +K ++ AL ++ S + ++ + +Y + AA L+ +G+ R SV I N PEWF FG + AG V +G+Y +N+ E V ++ E V +V + + + K + + P LKA++ G
Sbjct: 12 STSLHDAVKLRLDEPSSFSQTVPPQTIPEFFKESCEKYSDLPALVWETPGSGNDGWTTLTFGEYQERVEQAALMLLSVGVEERSSVGILAFNCPEWFFAEFGALRAGAVVAGVYPSNSAEAVHHVLATGESSVCVVDDAQQMAKLRAIKERLPRLKAVIQLHG 174
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Match: CG3961 (gene:FBgn0036821 transcript:FBtr0302322) HSP 1 Score: 159.458 bits (402), Expect = 1.616e-40 Identity = 128/453 (28.26%), Postives = 220/453 (48.57%), Query Frame = 1
Query: 838 ECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM---KFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
+ C++ YTSGTTGNPKGVM++H N+V ++ M + R + ++++S+LPL+H+ + C+ + + F D + L + LK + PT VPR+ ++ ++++ I S +K+ + + A + +++ NG + ++F KVH+A G + L + +APL+ +VL + +L ++ E YG +EC+G T+ Q + VG + KL + GE+C+ G +VF GY PEKT E DSE W H+GDVG + G L I R K + + GE I P IE I +++ V G+ K C+I V +P D+ K+W + T +S + +KN K + +++ + ++ + +V+ P FSV G L PT K KR + + + +Y+
Sbjct: 226 DLCTVCYTSGTTGNPKGVMLTHGNVVAGVCSVILQMGDHRIR-AGDVMVSFLPLAHMFERCCENGM-YYVGGCVGFYSGDIKE--LTNDLKMLKPTVMPAVPRLLNRVYDKIQN-DISASGLKRGLFNMAMRAKEKEIARGVLRRNGCWDK-----LVFKKVHQAFGGNLRLMVV-GSAPLAGNVLTFMRCALGCLVLEGYGQTECTGAITLTVQGDHVPNHVGPPVSCNAVKLVDVPEMEYFANQNTGEVCVRGSNVFHGYYKDPEKTAEAIDSEGWHHTGDVGMWLPNGTLRIIDRRKHIFKLSQGEYIVPEKIEN-IYTLSQYVNQVYVYGESLK--------SCIIAVV--VPDTDVL---KQWATENNVRGT-LSVLCNNKNVK-ELIMNDMLNWGKQSGLKSFEQVKDIYLHPDPFSVQNGLLTPTFKAKRPQLKSYFKPQLEDMYK 651
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Match: CG3961 (gene:FBgn0036821 transcript:FBtr0333712) HSP 1 Score: 159.458 bits (402), Expect = 1.943e-40 Identity = 128/453 (28.26%), Postives = 220/453 (48.57%), Query Frame = 1
Query: 838 ECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM---KFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
+ C++ YTSGTTGNPKGVM++H N+V ++ M + R + ++++S+LPL+H+ + C+ + + F D + L + LK + PT VPR+ ++ ++++ I S +K+ + + A + +++ NG + ++F KVH+A G + L + +APL+ +VL + +L ++ E YG +EC+G T+ Q + VG + KL + GE+C+ G +VF GY PEKT E DSE W H+GDVG + G L I R K + + GE I P IE I +++ V G+ K C+I V +P D+ K+W + T +S + +KN K + +++ + ++ + +V+ P FSV G L PT K KR + + + +Y+
Sbjct: 238 DLCTVCYTSGTTGNPKGVMLTHGNVVAGVCSVILQMGDHRIR-AGDVMVSFLPLAHMFERCCENGM-YYVGGCVGFYSGDIKE--LTNDLKMLKPTVMPAVPRLLNRVYDKIQN-DISASGLKRGLFNMAMRAKEKEIARGVLRRNGCWDK-----LVFKKVHQAFGGNLRLMVV-GSAPLAGNVLTFMRCALGCLVLEGYGQTECTGAITLTVQGDHVPNHVGPPVSCNAVKLVDVPEMEYFANQNTGEVCVRGSNVFHGYYKDPEKTAEAIDSEGWHHTGDVGMWLPNGTLRIIDRRKHIFKLSQGEYIVPEKIEN-IYTLSQYVNQVYVYGESLK--------SCIIAVV--VPDTDVL---KQWATENNVRGT-LSVLCNNKNVK-ELIMNDMLNWGKQSGLKSFEQVKDIYLHPDPFSVQNGLLTPTFKAKRPQLKSYFKPQLEDMYK 663
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:ZFIN;Acc:ZDB-GENE-030131-7099]) HSP 1 Score: 519.62 bits (1337), Expect = 4.007e-174 Identity = 247/477 (51.78%), Postives = 332/477 (69.60%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM---MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL---DSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L ++ PN+CC+LIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM DI +P+ +FAQPDALKGSL +TL+E+ PT F+GVPRV+EK+ E+MK + S+V++ ++SWAKD+G +TNL KM P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE SGP TI N FR S GK++P GCK + +D K GEIC +GRHVFMGY+N P+KT+E D+E WLHSGD+G ++ +LFITGRIKEL+ITAGGENI PVPIE +KE + ISNAM++GDKRK+L +LLT+KC I+ T +P D+LTP A + +G + T +++I ++ + +I +GI R NE A S + +QKWT L DFS+PGGELGPT+KLKR VV ++Y I S Y+
Sbjct: 305 WAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIP--GCKTKLSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTMKLKRPVVMKMYKEQIESFYK 779 HSP 2 Score: 113.62 bits (283), Expect = 4.007e-174 Identity = 59/166 (35.54%), Postives = 94/166 (56.63%), Query Frame = 2
Query: 167 PVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
P + +++T D KLRM G L P+T++ MF + + + + AL +K ++ + ++ YS+YY+ C AAKS +++GL V I G NS EWFI I AGG A GIY TN+ E +++ NC+ + +V N + L+K + + P+LKAI+ Y
Sbjct: 129 PTKGTELWTTHRDGEVKLRMAESG-LGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQ--WKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQY 291
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:ZFIN;Acc:ZDB-GENE-030131-7099]) HSP 1 Score: 519.235 bits (1336), Expect = 4.008e-174 Identity = 247/477 (51.78%), Postives = 332/477 (69.60%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM---MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL---DSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L ++ PN+CC+LIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM DI +P+ +FAQPDALKGSL +TL+E+ PT F+GVPRV+EK+ E+MK + S+V++ ++SWAKD+G +TNL KM P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE SGP TI N FR S GK++P GCK + +D K GEIC +GRHVFMGY+N P+KT+E D+E WLHSGD+G ++ +LFITGRIKEL+ITAGGENI PVPIE +KE + ISNAM++GDKRK+L +LLT+KC I+ T +P D+LTP A + +G + T +++I ++ + +I +GI R NE A S + +QKWT L DFS+PGGELGPT+KLKR VV ++Y I S Y+
Sbjct: 253 WAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIP--GCKTKLSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTMKLKRPVVMKMYKEQIESFYK 727 HSP 2 Score: 114.005 bits (284), Expect = 4.008e-174 Identity = 59/166 (35.54%), Postives = 94/166 (56.63%), Query Frame = 2
Query: 167 PVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
P + +++T D KLRM G L P+T++ MF + + + + AL +K ++ + ++ YS+YY+ C AAKS +++GL V I G NS EWFI I AGG A GIY TN+ E +++ NC+ + +V N + L+K + + P+LKAI+ Y
Sbjct: 77 PTKGTELWTTHRDGEVKLRMAESG-LGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQ--WKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQY 239
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Match: acsbg1 (acyl-CoA synthetase bubblegum family member 1 [Source:ZFIN;Acc:ZDB-GENE-110914-219]) HSP 1 Score: 421.779 bits (1083), Expect = 2.297e-145 Identity = 214/474 (45.15%), Postives = 304/474 (64.14%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLD----SEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W EFM LG+ ++D L ++ N+CC LIYTSGTTG+PKGVM+SHDN+ WTA + + +E L+SYLPLSH+AAQ+ D+ + I FAQPDALKG+L +L+EV PT +GVPRV+EKIME++KE +K+ + +WA + N E F +++A+ +I K+ LGF C+ FS AAP+ + L +F L++ + E YGMSE SGP + ++F S GK++P GC+ + DG GE+C +GR+VFMG++N +KTKE D + WL SGD+G +++G+++I GRIKEL+ITAGGENI P+PIE +K+ L ISNAM++GD+RK+L +LLT+KC ++P T PTD L+ A + IG T +S I K+ + SI++GI + N A S + +QKWT L DFSV GGELGPT+KL+R VV ++Y I + Y
Sbjct: 202 WEEFMELGLEVSDHELDEVISKQRANQCCVLIYTSGTTGSPKGVMLSHDNITWTAHHASRAGDMQPAEIRQESLVSYLPLSHIAAQIYDLWTGIKWGEQISFAQPDALKGTLVDSLREVTPTAHMGVPRVWEKIMEKIKEGISRCGYMKRKMVTWAMSVSLDANQRLKKDEEKSFLFTLAEKLILQKLRAELGFSSCVKFFSGAAPIGRETLQFFLGLNIRLYEAYGMSETSGPHFMSGPEAYQFLSCGKVVP--GCQYKLININADGS-GEVCFWGRNVFMGFLNLEDKTKEALDEDGWLRSGDLGKVDEDGFIYINGRIKELIITAGGENIPPLPIEDAVKQELSIISNAMLIGDERKFLSMLLTLKCTVNPETTEPTDILSLEAVEFCQRIGSQSTKLSDITGGKDKLVYKSIEDGIGQVNSKATSNAQRIQKWTILDKDFSVAGGELGPTMKLRRPVVLQMYHNEIENFY 672 HSP 2 Score: 115.546 bits (288), Expect = 2.297e-145 Identity = 63/174 (36.21%), Postives = 99/174 (56.90%), Query Frame = 2
Query: 176 TENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYS---QESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSI 688
+++++T +LRM+ + +PIT+H MF+N++ K N AL K ++++FL +YY LC MAAK +++GL V I G NS EWFI GT+FAGG+ +GIY TN+ + + N+ V +V N++ L K + V P+LKAIV Y G + + +
Sbjct: 31 AQSLWTTEASGSVQLRMD---EHCPEQPITVHQMFYNSVQKYGNLTALASKRGNKWEKATFL-----EYYHLCRMAAKGFLKLGLEQFHGVAILGFNSAEWFIAAVGTVFAGGIMAGIYTTNSPDACLHVANDSRANVIVVENQKQLDKIMQVKDKLPHLKAIVQYSGSLKEKL 196
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Match: acsl5 (acyl-CoA synthetase long chain family member 5 [Source:ZFIN;Acc:ZDB-GENE-040912-169]) HSP 1 Score: 157.147 bits (396), Expect = 1.031e-41 Identity = 138/477 (28.93%), Postives = 225/477 (47.17%), Query Frame = 1
Query: 784 ININDDTLIGRLNHLIP----NECCSLI-YTSGTTGNPKGVMMSHDNLVWTAECMLYYMK---FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTP--AAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
+++ D +G+ NH P E S+I +TSGTTG+PKG M++H+N+V A ++ + ++ IS+LPL+H+ ++ ++ + F Q D L +K + PT F VPR+ ++ ++++ S K W + A++ K+ S+ +IF+KV ++LG R + + AAP+S VL + A L I E YG +EC+ + +++ G VG +P KL D E+ EGEIC+ G +VF GY+ PEKT E D + WLH+GD+G + G L I R K + A GE IAP IE + ++ V G+ + V + +P ++ P A K G S+ ++ ++ K +I I + K E + +V+ P F++ G L PTLK KR + + + I SLY
Sbjct: 231 LSLKDVEALGKNNHHRPVPPKPEDLSIICFTSGTTGDPKGAMLTHENVVADAAGVVRTFESVFVPVPSDVSISFLPLAHMFERVVQTVL-YGVGGRVGFFQGDIR--LLPDDMKALQPTVFPVVPRLLNRVYDKVQ------SGAKTPFKKWLLNFAIEKKCAEV-KQGIIRNDSMWDKLIFHKVQESLG-GRVRVMVTGAAPISPSVLTFLRACLGCQIFEAYGQTECTAACSFTIPGDWKTGHVGAPIPCNTIKLVDVEEMDYFASNGEGEICVKGTNVFRGYLGDPEKTAEALDKDGWLHTGDIGKWLPSGVLKIIDRKKNIFKLAQGEYIAPEKIENVYIRSAP-VAQVFVHGESLQSSLVAIV----------VPDPEVLPGFAEKLGLKG------SLEELCRNQELKKAI-ISDLNKLGREAGLKSFEQVKDLYLHPDMFTIENGLLTPTLKAKRADLTKYFRVQIESLY 678 HSP 2 Score: 34.2686 bits (77), Expect = 1.031e-41 Identity = 36/154 (23.38%), Postives = 54/154 (35.06%), Query Frame = 2
Query: 263 TLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKS--VCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVS--KNCPNLKAIVYYGGLIDDSIKGGCKISL 712
TL+ +F + N L ++ + + + Y Q LI G + + I N PEW I VA +Y T E + FIIN I ++ + + S K P LK I+ + G KI L
Sbjct: 76 TLYEVFQRGLRVSGNGPCLGFR-KKGQPYQWLKYKQVSDRAEFLGSGLIHRGQKPSQDTFIGIFAQNRPEWIIAELACYTYSMVAVPLYDTLGPEALVFIINRANISTVICDKKDKAETLLDNSEKKLTPLLKTIILMDSYDSALTERGSKIGL 228
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Match: acsl5 (acyl-CoA synthetase long chain family member 5 [Source:ZFIN;Acc:ZDB-GENE-040912-169]) HSP 1 Score: 157.147 bits (396), Expect = 1.031e-41 Identity = 138/477 (28.93%), Postives = 225/477 (47.17%), Query Frame = 1
Query: 784 ININDDTLIGRLNHLIP----NECCSLI-YTSGTTGNPKGVMMSHDNLVWTAECMLYYMK---FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTP--AAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
+++ D +G+ NH P E S+I +TSGTTG+PKG M++H+N+V A ++ + ++ IS+LPL+H+ ++ ++ + F Q D L +K + PT F VPR+ ++ ++++ S K W + A++ K+ S+ +IF+KV ++LG R + + AAP+S VL + A L I E YG +EC+ + +++ G VG +P KL D E+ EGEIC+ G +VF GY+ PEKT E D + WLH+GD+G + G L I R K + A GE IAP IE + ++ V G+ + V + +P ++ P A K G S+ ++ ++ K +I I + K E + +V+ P F++ G L PTLK KR + + + I SLY
Sbjct: 231 LSLKDVEALGKNNHHRPVPPKPEDLSIICFTSGTTGDPKGAMLTHENVVADAAGVVRTFESVFVPVPSDVSISFLPLAHMFERVVQTVL-YGVGGRVGFFQGDIR--LLPDDMKALQPTVFPVVPRLLNRVYDKVQ------SGAKTPFKKWLLNFAIEKKCAEV-KQGIIRNDSMWDKLIFHKVQESLG-GRVRVMVTGAAPISPSVLTFLRACLGCQIFEAYGQTECTAACSFTIPGDWKTGHVGAPIPCNTIKLVDVEEMDYFASNGEGEICVKGTNVFRGYLGDPEKTAEALDKDGWLHTGDIGKWLPSGVLKIIDRKKNIFKLAQGEYIAPEKIENVYIRSAP-VAQVFVHGESLQSSLVAIV----------VPDPEVLPGFAEKLGLKG------SLEELCRNQELKKAI-ISDLNKLGREAGLKSFEQVKDLYLHPDMFTIENGLLTPTLKAKRADLTKYFRVQIESLY 678 HSP 2 Score: 34.2686 bits (77), Expect = 1.031e-41 Identity = 36/154 (23.38%), Postives = 54/154 (35.06%), Query Frame = 2
Query: 263 TLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKS--VCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVS--KNCPNLKAIVYYGGLIDDSIKGGCKISL 712
TL+ +F + N L ++ + + + Y Q LI G + + I N PEW I VA +Y T E + FIIN I ++ + + S K P LK I+ + G KI L
Sbjct: 76 TLYEVFQRGLRVSGNGPCLGFR-KKGQPYQWLKYKQVSDRAEFLGSGLIHRGQKPSQDTFIGIFAQNRPEWIIAELACYTYSMVAVPLYDTLGPEALVFIINRANISTVICDKKDKAETLLDNSEKKLTPLLKTIILMDSYDSALTERGSKIGL 228
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:100158533]) HSP 1 Score: 486.108 bits (1250), Expect = 8.486e-168 Identity = 235/472 (49.79%), Postives = 321/472 (68.01%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM + Y +AK ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT+E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +KE + ISNAMV+GDK+K+L +LLT+KC ++ T P D+LTP A + IG T +S I+ K+ + +I +G+ N+ A S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 189 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKMNGTHILKRYHVAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVPIISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVSDIVGGKDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQ 660 HSP 2 Score: 126.716 bits (317), Expect = 8.486e-168 Identity = 67/178 (37.64%), Postives = 99/178 (55.62%), Query Frame = 2
Query: 137 KITFEIKQHSPVETENM--FSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
K F I Q + T +M ++T D +LRM G +A + P+T+H +F + + K + AL K Q + + Y QYY+ C +AAK +++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + + P+LKAI+ Y
Sbjct: 1 KTVFFISQEGNLSTSDMKLWTTRRDNAVELRMEDSG-VASLPPVTIHQLFQDTVKKYGDYVALASK--QGDQWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQIQDQLPHLKAIIQY 175
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:100158533]) HSP 1 Score: 488.034 bits (1255), Expect = 1.054e-167 Identity = 238/476 (50.00%), Postives = 323/476 (67.86%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM NG P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT+E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +KE + ISNAMV+GDK+K+L +LLT+KC ++ T P D+LTP A + IG T +S I+ K+ + +I +G+ N+ A S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 193 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKM---NGSTPHPMKYHVAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVPIISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVSDIVGGKDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQ 665 HSP 2 Score: 124.405 bits (311), Expect = 1.054e-167 Identity = 67/186 (36.02%), Postives = 103/186 (55.38%), Query Frame = 2
Query: 107 VNGPLNKSQNKITFEIKQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
N L++S+N T ++K +++T D +LRM G +A + P+T+H +F + + K + AL K Q + + Y QYY+ C +AAK +++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + + P+LKAI+ Y
Sbjct: 6 TNNRLSESRNLSTSDMK---------LWTTRRDNAVELRMEDSG-VASLPPVTIHQLFQDTVKKYGDYVALASK--QGDQWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQIQDQLPHLKAIIQY 179
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:100158533]) HSP 1 Score: 487.649 bits (1254), Expect = 1.308e-167 Identity = 238/476 (50.00%), Postives = 323/476 (67.86%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM NG P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT+E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +KE + ISNAMV+GDK+K+L +LLT+KC ++ T P D+LTP A + IG T +S I+ K+ + +I +G+ N+ A S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 193 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKM---NGSTPHPMKYHVAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVPIISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVSDIVGGKDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQ 665 HSP 2 Score: 124.405 bits (311), Expect = 1.308e-167 Identity = 62/165 (37.58%), Postives = 94/165 (56.97%), Query Frame = 2
Query: 170 VETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
E + +++T D +LRM G +A + P+T+H +F + + K + AL K Q + + Y QYY+ C +AAK +++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + + P+LKAI+ Y
Sbjct: 18 AELQYLWTTRRDNAVELRMEDSG-VASLPPVTIHQLFQDTVKKYGDYVALASK--QGDQWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQIQDQLPHLKAIIQY 179
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:100158533]) HSP 1 Score: 488.804 bits (1257), Expect = 1.611e-167 Identity = 238/476 (50.00%), Postives = 323/476 (67.86%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM NG P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT+E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +KE + ISNAMV+GDK+K+L +LLT+KC ++ T P D+LTP A + IG T +S I+ K+ + +I +G+ N+ A S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 215 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKM---NGSTPHPMKYHVAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVPIISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVSDIVGGKDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQ 687 HSP 2 Score: 123.25 bits (308), Expect = 1.611e-167 Identity = 70/189 (37.04%), Postives = 103/189 (54.50%), Query Frame = 2
Query: 128 SQNKITFEIKQHS----PVE----TENM--FSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
S+N I EI + P E T +M ++T D +LRM G +A + P+T+H +F + + K + AL K Q + + Y QYY+ C +AAK +++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + + P+LKAI+ Y
Sbjct: 16 SENPIVTEITSAAAMLHPAEGNLSTSDMKLWTTRRDNAVELRMEDSG-VASLPPVTIHQLFQDTVKKYGDYVALASK--QGDQWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQIQDQLPHLKAIIQY 201
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:100158533]) HSP 1 Score: 488.804 bits (1257), Expect = 1.624e-167 Identity = 238/476 (50.00%), Postives = 323/476 (67.86%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM NG P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT+E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +KE + ISNAMV+GDK+K+L +LLT+KC ++ T P D+LTP A + IG T +S I+ K+ + +I +G+ N+ A S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 274 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKM---NGSTPHPMKYHVAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVPIISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVSDIVGGKDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQ 746 HSP 2 Score: 122.865 bits (307), Expect = 1.624e-167 Identity = 70/189 (37.04%), Postives = 103/189 (54.50%), Query Frame = 2
Query: 128 SQNKITFEIKQHS----PVE----TENM--FSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
S+N I EI + P E T +M ++T D +LRM G +A + P+T+H +F + + K + AL K Q + + Y QYY+ C +AAK +++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + + P+LKAI+ Y
Sbjct: 75 SENPIVTEITSAAAMLHPAEGNLSTSDMKLWTTRRDNAVELRMEDSG-VASLPPVTIHQLFQDTVKKYGDYVALASK--QGDQWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQIQDQLPHLKAIIQY 260
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Match: Acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:MGI Symbol;Acc:MGI:3587728]) HSP 1 Score: 447.973 bits (1151), Expect = 1.043e-147 Identity = 224/474 (47.26%), Postives = 313/474 (66.03%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRH---SKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK-LDSEDGKEG--EICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I + L + N+C ++YTSGTTG PKGV++SHDN+ WTA M M+ + ++SYLPLSH+AAQ+ DI IP+ A FAQPDAL+G+L +TL+EV PT F+GVPR++EK+ + +KE S ++K +WAK +G + N +M+ K + P Y +AK ++F KV +LG D C FS+A+PLS DV +F SLD+ I EIYGMSECSGP T+ ++ +R S GK+L GCK + KEG E+CM+GRHVFMGY+N E T E D WLHSGD+G + +L+ITGRIKE+LITAGGEN++P+PIE +KE + IS+AM+VGDK K+LC+LLT+KC D + P + L+ AK + +G T++S I+ ++ + +I GI N+ A+S S ++KW L DFS+ GGELGPT KLKR+V+ + Y A I+S+Y
Sbjct: 194 WNDFMELGNDIPNIQLDRVILSQKANQCAVILYTSGTTGTPKGVLLSHDNITWTAGAMSQEMEINRVSGKQNTIVSYLPLSHIAAQLTDIWIPIKIGALTFFAQPDALRGTLVYTLQEVKPTLFMGVPRIWEKMQDTIKENVARSSRLRKKAFAWAKMLGLKVNTKRMLGKRDIPMNYRMAKALVFAKVRTSLGLDNCHAFFSSASPLSQDVSEFFLSLDIPIGEIYGMSECSGPHTVSNKSVYRVLSCGKVLS--GCKNMLYNQNKEGVGEVCMWGRHVFMGYLNKEEATLEALDENGWLHSGDIGRLDSHDFLYITGRIKEILITAGGENVSPIPIETLVKEKIPIISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQMLGSQATTVSDILKSRDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPTSKLKRSVITQKYKAQIDSMY 665 HSP 2 Score: 97.0561 bits (240), Expect = 1.043e-147 Identity = 56/162 (34.57%), Postives = 92/162 (56.79%), Query Frame = 2
Query: 182 NMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKN-CPNLKAIVYY 664
++ST D LR++ G P+T+ +F + ++ + AL K ++ + ++ +SQYY++C AAKSLI++GL + V I G NS EW +T GTI AGG+ GIY TN+ E +++I + IV N++ L+K + + + +KAIV Y
Sbjct: 22 RLWSTHCDGEVLLRLSKHGP-GHETPMTIPELFQESAERFSAYPALASKNGKK--WDTLTFSQYYEMCRKAAKSLIKLGLQRFQCVGILGFNSVEWVVTALGTILAGGLCVGIYATNSAEACQYVIQQANVSILIVENDQQLQKILLIPPDKMETVKAIVQY 180
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Match: Acsbg1 (acyl-CoA synthetase bubblegum family member 1 [Source:MGI Symbol;Acc:MGI:2385656]) HSP 1 Score: 421.394 bits (1082), Expect = 7.069e-147 Identity = 205/470 (43.62%), Postives = 306/470 (65.11%), Query Frame = 1
Query: 766 EFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
E + LG + ++ L ++ PN+CC L+YTSGTTGNPKGVM+S DN+ WTA + + +E+++SYLPLSH+AAQ+ D+ + A + FA PDALKG+L +TL+EV PT +GVPRV+EKIME+++E+A +++ + WA + NL + PF +A ++ +V +ALGF +C F AAP++ + +F L++ + YG+SE +GP + +R S G+++P KL ++D GEIC++GR +FMGY+N +KT E DSE WLH+GD+G + +G+L+ITGR+KEL+ITAGGEN+ PVPIE+ +K L IS+AM++GD+RK+L +LLT+KC +DP T PTD LT A + +G +++S+I+ ++ + +I EGI+R N NA +R +QKW L DFS+ GGELGPT+KLKR V E Y +I+S Y+
Sbjct: 248 ELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQ 717 HSP 2 Score: 121.324 bits (303), Expect = 7.069e-147 Identity = 60/162 (37.04%), Postives = 97/162 (59.88%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
E +++T D +LR+ +P T+H MF+ A+DK N AL +K ++ + I+Y QYY + AK +++GL SV I G NSPEWF + GT+FAGG+ +GIY T++ E ++I ++C V +V ++ L+K + + K+ P+LKA+V Y
Sbjct: 76 EALWTTRADGRVRLRLE---PFCTQRPYTVHQMFYEALDKYGNLSALGFK--RKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIY 232
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Match: Acsbg3 (acyl-CoA synthetase bubblegum family member 3 [Source:MGI Symbol;Acc:MGI:1925875]) HSP 1 Score: 321.627 bits (823), Expect = 2.265e-103 Identity = 163/483 (33.75%), Postives = 272/483 (56.31%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFR---HSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALK----------GSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE-NGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W F++L I+D+ L ++ L PN+CC+L+Y GTTG K +M+SHDN+ WT ++ + F+ +E+L+SYLPL Q+ D+ + + T++F D+ K G L L+EV PT F G+P V++++++ +K +D ++ ++ I WA +G TN MM E + P + +AK + F + K LG + C + L L +F S+++ I E+YG+SEC+G T+ +R S GK LP K++ E+ G +C++GRH+FMGY+ + T+ D+ WLH+ D+G + + +L++ G +L+ + GE + P PIE++++ + + AM+VG YLC LLT+KC I+P T +LT + T +S ++ D++P ++ I++GI+ N A + A++ KW L +DFSV GGELGP K+ R++V ++Y I LYE
Sbjct: 219 WKGFLDLADGISDEKLDKIIDTLKPNQCCALVYNQGTTGPSKAIMLSHDNITWTTAAIVQSLGFKCPPQGQEILVSYLPLCFPGIQILDVWVAISVAGTVYFPSLDSGKWSGLPRTPGTGFLMELLREVQPTTFCGIPWVWDRMLDSLKTKFLDSTAFRRRIDHWAMRMGLHTNKKLMMGEIHQPLCFGLAKRLTFERARKFLGLNHCQQFLNMGMGLPSGTLDFFLSVNIPIMELYGLSECTGLHTVSNLQAYRILSAGKALPKTHTKVEKENKDGIGNLCIWGRHIFMGYLRDKQSTERKVDTHGWLHTNDLGFLDFDKFLYVMGNNNDLIKLSSGEMVNPYPIEERVRTRIPIVRYAMLVGQDAPYLCALLTLKCQINPETGEARSNLTSEVVACCRKLRSQSTWLSDVLYDRDPLVTEFINQGIEDVNSEAPTMGAKIIKWVILDNDFSVDGGELGPMSKMNRSIVVKIYQEEIQKLYE 701 HSP 2 Score: 75.8702 bits (185), Expect = 2.265e-103 Identity = 48/150 (32.00%), Postives = 77/150 (51.33%), Query Frame = 2
Query: 215 KLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
+LRM K L P+T+ + +A K ++ A+ KY + L+ Y +YY+ C AAK+ +++GL V I G NS EW I G I AGG++ GI + + + + I E + +V N+ L+K + +LKAI+ Y
Sbjct: 59 QLRMG-KDPLDNEPPVTVPDLIMSAATKYSHYLAIGSKYKKSWQLLT--YIEYYEACRRAAKAFLKVGLERFHGVGIMGINSSEWVIASIGAIMAGGISVGILSSISPKACQVIAETSEMDIFVVDNDRQLQKINQIQGYLKHLKAIIQY 205
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Match: Acsl5 (acyl-CoA synthetase long-chain family member 5 [Source:MGI Symbol;Acc:MGI:1919129]) HSP 1 Score: 163.31 bits (412), Expect = 1.120e-45 Identity = 140/458 (30.57%), Postives = 226/458 (49.34%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK--FR-HSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTP--AAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFL-PHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
P + + +TSGTTG+PKG M++H+N+V L +++ F+ S ++ ISYLPL+H+ ++ I+ C I F Q D L +K + PT F VPR+ ++ ++++ A + +KK + + A + +++ + S+ ++F+K+ +LG L I + AAP+S VL +F A++ + E YG +EC+G +I ++ G VG + KL D D EGEIC+ G +VF GY+ PEKT+EV D + WLH+GD+G + G L I R K + A GE IAP IE + + V G+ + + + +P D P AAK G S ++ KN + +I E +++ + +S E K F+ P F++ G L PTLK KR + + + I SLYE
Sbjct: 252 PEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGIL-FSCGGKIGFFQGDIR--LLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAK--TPLKKFLLNLAIISKFNEVKNGIIRRD-----SLWDKLVFSKIQGSLGGKVRLMI-TGAAPISTPVLTFFRAAMGCWVFEAYGQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPEKIENVYSRSRPVL-QVFVHGESLRSFLIGVV----------VPDPDSLPSFAAKIGVKG------SFEELC--KNQCVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQIKSLYE 679 HSP 2 Score: 41.2022 bits (95), Expect = 1.120e-45 Identity = 43/161 (26.71%), Postives = 62/161 (38.51%), Query Frame = 2
Query: 263 TLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKS--VCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYV-CVSKN-CPNLKAIVYYGGLIDDSIKGGCK-----ISLYD 718
TL+ F + N L Y+ + + I+Y Q L+ G + + V I N PEW I+ VA +Y T E + F+IN + V I + V V K P LK I+ DD +K G K +SL+D
Sbjct: 76 TLYENFQRGLAVSDNGPCLGYRKPNQP-YKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHD 235
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Match: Acsl1 (acyl-CoA synthetase long-chain family member 1 [Source:MGI Symbol;Acc:MGI:102797]) HSP 1 Score: 162.155 bits (409), Expect = 2.700e-43 Identity = 138/468 (29.49%), Postives = 219/468 (46.79%), Query Frame = 1
Query: 808 IGRLNHLIPN-----ECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFR---HSKEMLISYLPLSHVAAQMCDIIIPLLCQ-ATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSEDGK------EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
+GR+N + P + + +TSGTTGNPKG M++H N++ + + ++ + ISYLPL+H+ Q + +LC A I F Q D L LK + PT F VPR+ ++ +++ F S+ W D + A++ + S+ +IF+K+ +LG L I + AAP+S VL + +L E YG +EC+ + ++ G VG +P KL D E+ EGE+C+ G +VF GY+ P +T E D + WLH+GD+G + G L I R K + A GE IAP IE I + ++ V G+ + I V+ V +P + A KR G S ++ +K+ +I +D+ +K E + +V+ P FS+ G L PTLK KR + + + I+ LY
Sbjct: 255 LGRVNRVKPKPPEPEDLAIICFTSGTTGNPKGAMITHQNIINDCSGFIKATESALTLNASDTQISYLPLAHMYEQQLQCV--MLCHGAKIGFFQGDIR--LLMDDLKVLQPTIFPVVPRLLNRMFDRI------FGQANTSLKRWLLDFASKRKEAEL-RSGIVRNNSLWDKLIFHKIQSSLGGKVRLMI-TGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYVKLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIEN-IYLRSEAVAQVFVHGESLQ----AFLIAVVVPDVESLP----SWAQKRGLQG------SFEELCRNKDINKAI-LDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELY 694 HSP 2 Score: 34.6538 bits (78), Expect = 2.700e-43 Identity = 36/151 (23.84%), Postives = 57/151 (37.75%), Query Frame = 2
Query: 263 TLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLN--ARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYV--CVSKNCPNLKAIVYYGGLIDDSIKGGCK 703
T++ F I N L + + + I+Y + +L LIQ G + + + + N PEW I G V +Y T + + +I+N E V E K + +K P LK IV D ++ G K
Sbjct: 92 TMYDGFQRGIQVSNNGPCLGSRKPNQP-YEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPLYDTLGADAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDLVERGKK 241
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Match: sp|Q7ZYC4|ACBG2_XENLA (Long-chain-fatty-acid--CoA ligase ACSBG2 OS=Xenopus laevis OX=8355 GN=acsbg2 PE=2 SV=1) HSP 1 Score: 482.256 bits (1240), Expect = 8.509e-166 Identity = 236/476 (49.58%), Postives = 320/476 (67.23%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG +I D L ++ PN+CC+LIYTSGTTG PKGVM+SHDN+ WTA ++ R + +E+++SYLPLSH+AAQM DI + + +FAQPDALKGSL TL+EV PT F+GVPRV+EK+ E+MK + S++K+ +++WAK +G TNL KM NG P Y +A ++F KV KALG DRC ++ AAP++ D L +F SL++ + E+YGMSE SGP TI + FR S GK++ K+ D GEI +GRHVFMGY+N +KT E D E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVP E +KE + ISNAM++GDK+K+L +LLT+KC ++ T P D+LTP A ++ IG T +S I+ K+ + +I EG+ NE + S + +VQKW L DFS+ GGELGPT+KLKR VV ++Y I+S Y+
Sbjct: 254 WKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAAAAGKTVRLREATDLQEIVVSYLPLSHIAAQMIDIWLTMKYGGATYFAQPDALKGSLAITLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKMATWAKGVGLETNLKKM---NGSTPHPMKYHVANKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDSDGSGEILFWGRHVFMGYLNMEDKTHESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPTEDAVKEQVPIISNAMLIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIQFCRQIGSKATLVSDIVGGKDTAVYAAIQEGVNSVNEKSTSNAQKVQKWLILEKDFSITGGELGPTMKLKRPVVAKMYKDQIDSFYQ 726 HSP 2 Score: 125.946 bits (315), Expect = 8.509e-166 Identity = 73/191 (38.22%), Postives = 104/191 (54.45%), Query Frame = 2
Query: 122 NKSQNKITFEIKQ-----HSPVE---TENM--FSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
N S+ I EI HS VE T +M ++ D KLR+ D+A + P+T+H +F ++K + AL K Q + ++Y QYY+ C +AAK I++GL V I G NS EWFI G IFAGG A GIY TN+ E ++ NCE + +V N++ L+K + V P+LKAI+ Y
Sbjct: 53 NSSEKPIVPEISSSDVMVHSAVENLPTSDMKLWTAQRDSAVKLRLE-DSDVASLPPVTIHQLFQETVNKYGDYVALASK--QGDQWHKMSYKQYYEQCRIAAKGFIKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQVQDQLPHLKAIIQY 240
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Match: sp|Q5ZKR7|ACBG2_CHICK (Long-chain-fatty-acid--CoA ligase ACSBG2 OS=Gallus gallus OX=9031 GN=ACSBG2 PE=2 SV=2) HSP 1 Score: 471.855 bits (1213), Expect = 3.014e-159 Identity = 235/478 (49.16%), Postives = 319/478 (66.74%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWT---AECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL----DSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W EF++LG +I D L + PN+CC+LIYTSGTTG PKGVM+SHDNL WT A L ++ +E+++SYLPLSHVAAQM DI +P+ + FAQPDALKG+L TL+EV PT F+GVPRV+EKI E+MK + S++++ ++SWAK +G +TNL M NG P + +A+ +++ KV KA+G DRC F+ AAP+S + L +F SL++ + E+YGMSE SGP T+ FR S GK + GC+ DG GEIC GRH+FMGY+N EKTKE D + WLHSGD+G + +G+++ITGRIKEL+ITAGGEN+ PVPIE +KE ISNAM+VGDK K+L +LLT+KC+I+ + P DDLT A + +G T +S+II+ K+ + +I + N+ A+S + ++QKW L DFSV GGELGPT+KLKR VV + Y LI+ Y
Sbjct: 248 WREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWM---NGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMA--GCRTLIHKPDADGI-GEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFY 719 HSP 2 Score: 114.39 bits (285), Expect = 3.014e-159 Identity = 61/163 (37.42%), Postives = 91/163 (55.83%), Query Frame = 2
Query: 182 NMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANE-ELLKKYVCVSKNCPNLKAIVYYG 667
N+++T D KLRM+ +G + P T+H +F A+ K + AL K + ++ + Y YY C AAKS +++ L VCI G NSPEWFI G IFAGG+A GIY TN+ E ++ NC + +V N + +K + + P++KAI+ YG
Sbjct: 76 NVWTTQQDGEVKLRMDEEG-MGSEAPKTVHEVFQEAVSKYGDYYALASK--KNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQACRKSLEIEHKLPHMKAIIQYG 235
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Match: sp|Q96GR2|ACBG1_HUMAN (Long-chain-fatty-acid--CoA ligase ACSBG1 OS=Homo sapiens OX=9606 GN=ACSBG1 PE=1 SV=2) HSP 1 Score: 430.254 bits (1105), Expect = 8.264e-148 Identity = 214/470 (45.53%), Postives = 307/470 (65.32%), Query Frame = 1
Query: 766 EFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
EFM LG + ++ L ++ PN+CC L+YTSGTTGNPKGVM+S DN+ WTA R + +E+++SYLPLSH+AAQ+ D+ + A + FA+PDALKGSL +TL+EV PT +GVPRV+EKIME+++E+A +++ + WA + NL + PF +A ++ KV +ALGF +C F AAP+ + +F L++ + YG+SE SGP + +R S GK++P KL ++D + GEIC++GR +FMGY+N +KT E D E WLH+GD G + +G+L+ITGR+KEL+ITAGGEN+ PVPIE+ +K L ISNAM++GD+RK+L +LLT+KC +DP T TD+LT A + +G T++S+II K+ + +I+EGI+R N NA +R +QKW L DFS+ GGELGPT+KLKR V E Y +I+S Y+
Sbjct: 251 EFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKLVNQDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTMKLKRLTVLEKYKGIIDSFYQ 720 HSP 2 Score: 117.857 bits (294), Expect = 8.264e-148 Identity = 62/162 (38.27%), Postives = 97/162 (59.88%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
E +++T D +LR++ P T+H MF+ A+DK + AL +K ++ + I+YSQYY L AAK +++GL SV I G NSPEWF + GT+FAGG+ +GIY T++ E ++I +C V +V ++ L+K + + K P+LKA+V Y
Sbjct: 79 EALWTTRADGRVRLRIDPS---CPQLPYTVHRMFYEALDKYGDLIALGFK--RQDKWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILKIWKQLPHLKAVVIY 235
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Match: sp|Q2XU92|ACBG2_MOUSE (Long-chain-fatty-acid--CoA ligase ACSBG2 OS=Mus musculus OX=10090 GN=Acsbg2 PE=1 SV=1) HSP 1 Score: 447.973 bits (1151), Expect = 6.348e-147 Identity = 224/474 (47.26%), Postives = 313/474 (66.03%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRH---SKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMM-KENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK-LDSEDGKEG--EICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W +FM LG +I + L + N+C ++YTSGTTG PKGV++SHDN+ WTA M M+ + ++SYLPLSH+AAQ+ DI IP+ A FAQPDAL+G+L +TL+EV PT F+GVPR++EK+ + +KE S ++K +WAK +G + N +M+ K + P Y +AK ++F KV +LG D C FS+A+PLS DV +F SLD+ I EIYGMSECSGP T+ ++ +R S GK+L GCK + KEG E+CM+GRHVFMGY+N E T E D WLHSGD+G + +L+ITGRIKE+LITAGGEN++P+PIE +KE + IS+AM+VGDK K+LC+LLT+KC D + P + L+ AK + +G T++S I+ ++ + +I GI N+ A+S S ++KW L DFS+ GGELGPT KLKR+V+ + Y A I+S+Y
Sbjct: 194 WNDFMELGNDIPNIQLDRVILSQKANQCAVILYTSGTTGTPKGVLLSHDNITWTAGAMSQEMEINRVSGKQNTIVSYLPLSHIAAQLTDIWIPIKIGALTFFAQPDALRGTLVYTLQEVKPTLFMGVPRIWEKMQDTIKENVARSSRLRKKAFAWAKMLGLKVNTKRMLGKRDIPMNYRMAKALVFAKVRTSLGLDNCHAFFSSASPLSQDVSEFFLSLDIPIGEIYGMSECSGPHTVSNKSVYRVLSCGKVLS--GCKNMLYNQNKEGVGEVCMWGRHVFMGYLNKEEATLEALDENGWLHSGDIGRLDSHDFLYITGRIKEILITAGGENVSPIPIETLVKEKIPIISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQMLGSQATTVSDILKSRDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPTSKLKRSVITQKYKAQIDSMY 665 HSP 2 Score: 97.0561 bits (240), Expect = 6.348e-147 Identity = 56/162 (34.57%), Postives = 92/162 (56.79%), Query Frame = 2
Query: 182 NMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKN-CPNLKAIVYY 664
++ST D LR++ G P+T+ +F + ++ + AL K ++ + ++ +SQYY++C AAKSLI++GL + V I G NS EW +T GTI AGG+ GIY TN+ E +++I + IV N++ L+K + + + +KAIV Y
Sbjct: 22 RLWSTHCDGEVLLRLSKHGP-GHETPMTIPELFQESAERFSAYPALASKNGKK--WDTLTFSQYYEMCRKAAKSLIKLGLQRFQCVGILGFNSVEWVVTALGTILAGGLCVGIYATNSAEACQYVIQQANVSILIVENDQQLQKILLIPPDKMETVKAIVQY 180
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Match: sp|Q2KHW5|ACBG1_BOVIN (Long-chain-fatty-acid--CoA ligase ACSBG1 OS=Bos taurus OX=9913 GN=ACSBG1 PE=2 SV=1) HSP 1 Score: 430.639 bits (1106), Expect = 1.063e-146 Identity = 215/480 (44.79%), Postives = 312/480 (65.00%), Query Frame = 1
Query: 766 EFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE*YSDQIGHSI 2193
E M LG + ++ L +N PN+CC+L+YTSGTTGNPKGVM+S DN+ WTA + + +E+++SYLPLSH+AAQ+ D+ + A + FA+PDALKGSL +TL+EV PT +GVPRV+EKIMEQ++E+A + + + WA + NL + PF +A ++ KV +ALGF +C F AAP++ + +F L++ + YG+SE SGP + +R S GK++P KL +ED + GEIC++GR +FMGY+N +KT E D+E WLH+GD G + +G+L+ITGR+KEL+ITAGGEN+ PVPIE+ +K L I NAM++GD+RK+L +LLT+KC +DP T PTD+LT A + +G T++S+++ K+ + +I+EGI+R N NA +R +QKW L DFS+ GGELGPT+KLKR V E Y +I+S Y+ + G S+
Sbjct: 234 ELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKSKQGSSL 713 HSP 2 Score: 113.62 bits (283), Expect = 1.063e-146 Identity = 58/169 (34.32%), Postives = 99/169 (58.58%), Query Frame = 2
Query: 158 QHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
Q +P E E +++T D +LR++ + P T+H MF +DK + A+ +K ++ ++ I+Y+QYY L AAK +++GL SV I NSPEWF + G +F GG+ +GIY T++ E ++I +C + +V ++ L+K + + K+ P+LKA+V Y
Sbjct: 56 QLNPPE-EALWTTRADGRVRLRID---PICSQTPRTVHQMFSTTLDKYGDLSAMGFK--RQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIY 218
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Match: W5L9W3 (AMP-binding domain-containing protein OS=Astyanax mexicanus OX=7994 PE=4 SV=2) HSP 1 Score: 503.827 bits (1296), Expect = 2.815e-173 Identity = 244/471 (51.80%), Postives = 324/471 (68.79%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG-PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLD--SEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W +FM LG +I DD L +N NECCSLIYTSGTTGNPKGVM+SHDN+ W A + + +E L+SYLPLSHVAAQ+ D+ + + T HFA+PDALKGSL +TL++V PT F+GVPRV+EK+ E M+ + S++KK I+ WAK IG +TN M + P+G+ +A +IF +V ALG DRC F+ AAP++ + L YF SL++ + E+YGMSE +GP T+ ++N FR S GK++ KLD EDG GE+C +GRHVFMGY+N PEKT E D+E WLHSGD+G + +++ITGRIKEL+ITAGGEN+ PVPIE +KE + ISNAM+VGDK+K+L +LLT+KC +D PTD+LTP A + G T +S+II +K P + +I EG+ NE A S + VQKWT LP DFS+ GGELGPT+KLKR VV+++Y I+ YE
Sbjct: 298 WADFMKLGTDIADDQLDDLINSQNANECCSLIYTSGTTGNPKGVMLSHDNITWIANASGMMIGTKMKEESLVSYLPLSHVAAQVNDLWVSMKFAGTTHFAEPDALKGSLANTLRDVRPTSFLGVPRVWEKMQEAMQSIGAKNSAMKKRIADWAKGIGLQTNYNLMNGDTSVPWGFMLANNLIFKRVRAALGLDRCRTCFTGAAPITKETLEYFMSLNIQLYELYGMSESTGPHTLSWENNFRIMSCGKVIRGCKTKLDKPDEDGN-GEVCFWGRHVFMGYLNMPEKTDEALDTEGWLHSGDLGKLDQNDFMYITGRIKELIITAGGENVPPVPIEDALKEEVSIISNAMLVGDKKKFLAMLLTLKCKVDDNGD-PTDELTPLAVEYCQQRGVTATKVSEIINNKEPAIYKAIQEGVNIINEKATSNAQRVQKWTLLPRDFSISGGELGPTMKLKRPVVSKMYKDDIDKFYE 766 HSP 2 Score: 137.117 bits (344), Expect = 2.815e-173 Identity = 73/183 (39.89%), Postives = 109/183 (59.56%), Query Frame = 2
Query: 140 ITFEIKQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSI 688
ITFE++ S E +S++ DK KLRM G + P+T+H MF + ++K + AL++K + ++ ++ Y QYYQ C AAKS +++GL V I G NSPEWFI+ I AGG+ +GIY TN+ E +++ +NCE V +V N + L K + V P+LKAIV Y G ++ I
Sbjct: 116 ITFEVRLAS---AEQYWSSARDKAVKLRMAESGPGSEA-PLTIHQMFQDTVNKYGDHPALRWK--ENGAWTTLTYKQYYQHCRTAAKSFLKLGLERFHGVGILGFNSPEWFISNVACIMAGGLTTGIYTTNSAEACQYVAHNCEANVIVVENNKQLVKILQVKDQLPHLKAIVQYKGELERKI 292
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Match: A0A3P8WSG3 (AMP-binding domain-containing protein OS=Cynoglossus semilaevis OX=244447 PE=4 SV=1) HSP 1 Score: 506.908 bits (1304), Expect = 1.243e-171 Identity = 242/469 (51.60%), Postives = 328/469 (69.94%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEN-GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
W EFM LG ++ D+ L R++ L NECC+LIYTSGTTGNPKGVM+SHDNL WTA + + + E+L+SYLPLSHVAAQM DI + + T++FA PDALKGSLG TL+E PTYF+GVPRV+EK+ E+MK M + S +++ +++WAK IG + N + M EN P+GY +A ++F KV ALG DRC F+ AAP+S D L YF SL++ + E+YGMSE SGP T+ +++ S GK++P KL++ D + GEIC +GRH+FMGY+N +KT E D + WLHSGD+G + +LFITGRIKEL+ITAGGENI PVPIE K+K + +SN M++GDK K+L +LLT+KCV+D + PTDDL+P A + G T +S+II ++ P + +I EG++R N A S + +VQKW L DFSVPGGELGPT+KL+R +V ++Y IN +Y
Sbjct: 261 WEEFMKLGSDVPDEQLNARIDSLRANECCTLIYTSGTTGNPKGVMLSHDNLTWTARTASTLVDYSYGDEVLVSYLPLSHVAAQMVDIWLSMTFGGTVYFADPDALKGSLGTTLREARPTYFLGVPRVWEKMQEKMKAMGMKASPMRRRVANWAKSIGLQYNYSAMNGENVVPWGYMLANNLVFKKVRAALGLDRCKICFTGAAPISKDTLEYFMSLNIPLMELYGMSESSGPHTVSVNHDYSITSCGKVMPGCKTKLENPDNEGIGEICFWGRHIFMGYLNMVDKTAEALDQDGWLHSGDLGKHDRNKFLFITGRIKELIITAGGENIPPVPIEDKLKAEVPILSNVMLIGDKLKFLSMLLTLKCVMDDNGE-PTDDLSPEALDYCQQHGVTATKVSEIIANREPAIYKAIQEGLERVNAMATSNAQKVQKWVILDKDFSVPGGELGPTMKLRRPIVVKMYEDKINEMY 728 HSP 2 Score: 128.257 bits (321), Expect = 1.243e-171 Identity = 66/164 (40.24%), Postives = 100/164 (60.98%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
+ ++ST DK KLRM G +A P T+H +F A+++ + ALK K +E ++++ + +YY+ C AAKS +++GL V I G N+ EWFI+ G IFAGG+A+GIY TN+ E ++ NCE + +V N++ L K + V P+LKAIV Y G
Sbjct: 89 DQLWSTHRDKAVKLRMEGSG-IASETPRTVHQLFLEAVEQCGDYPALKSK--KEGQWVTLTWREYYKQCRAAAKSFLKLGLERYHGVGILGFNAAEWFISDIGCIFAGGLAAGIYTTNSPEACMYVAANCEANILVVENQKQLDKILQVKDQLPHLKAIVQYRG 249
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Match: B0S7E7 (Acyl-CoA synthetase bubblegum family member 2 (Fragment) OS=Danio rerio OX=7955 GN=acsbg2 PE=1 SV=1) HSP 1 Score: 519.62 bits (1337), Expect = 5.466e-171 Identity = 247/477 (51.78%), Postives = 332/477 (69.60%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM---MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL---DSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L ++ PN+CC+LIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM DI +P+ +FAQPDALKGSL +TL+E+ PT F+GVPRV+EK+ E+MK + S+V++ ++SWAKD+G +TNL KM P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE SGP TI N FR S GK++P GCK + +D K GEIC +GRHVFMGY+N P+KT+E D+E WLHSGD+G ++ +LFITGRIKEL+ITAGGENI PVPIE +KE + ISNAM++GDKRK+L +LLT+KC I+ T +P D+LTP A + +G + T +++I ++ + +I +GI R NE A S + +QKWT L DFS+PGGELGPT+KLKR VV ++Y I S Y+
Sbjct: 305 WAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIP--GCKTKLSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTMKLKRPVVMKMYKEQIESFYK 779 HSP 2 Score: 113.62 bits (283), Expect = 5.466e-171 Identity = 59/166 (35.54%), Postives = 94/166 (56.63%), Query Frame = 2
Query: 167 PVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
P + +++T D KLRM G L P+T++ MF + + + + AL +K ++ + ++ YS+YY+ C AAKS +++GL V I G NS EWFI I AGG A GIY TN+ E +++ NC+ + +V N + L+K + + P+LKAI+ Y
Sbjct: 129 PTKGTELWTTHRDGEVKLRMAESG-LGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQ--WKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQY 291
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Match: F1RCV2 (Acyl-CoA synthetase bubblegum family member 2 OS=Danio rerio OX=7955 GN=acsbg2 PE=1 SV=2) HSP 1 Score: 519.235 bits (1336), Expect = 5.468e-171 Identity = 247/477 (51.78%), Postives = 332/477 (69.60%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM---MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL---DSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L ++ PN+CC+LIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM DI +P+ +FAQPDALKGSL +TL+E+ PT F+GVPRV+EK+ E+MK + S+V++ ++SWAKD+G +TNL KM P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE SGP TI N FR S GK++P GCK + +D K GEIC +GRHVFMGY+N P+KT+E D+E WLHSGD+G ++ +LFITGRIKEL+ITAGGENI PVPIE +KE + ISNAM++GDKRK+L +LLT+KC I+ T +P D+LTP A + +G + T +++I ++ + +I +GI R NE A S + +QKWT L DFS+PGGELGPT+KLKR VV ++Y I S Y+
Sbjct: 253 WAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIP--GCKTKLSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTMKLKRPVVMKMYKEQIESFYK 727 HSP 2 Score: 114.005 bits (284), Expect = 5.468e-171 Identity = 59/166 (35.54%), Postives = 94/166 (56.63%), Query Frame = 2
Query: 167 PVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
P + +++T D KLRM G L P+T++ MF + + + + AL +K ++ + ++ YS+YY+ C AAKS +++GL V I G NS EWFI I AGG A GIY TN+ E +++ NC+ + +V N + L+K + + P+LKAI+ Y
Sbjct: 77 PTKGTELWTTHRDGEVKLRMAESG-LGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQ--WKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQY 239
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Match: A0A1S3T5V9 (long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X3 OS=Salmo salar OX=8030 GN=LOC106613839 PE=4 SV=1) HSP 1 Score: 511.916 bits (1317), Expect = 1.400e-170 Identity = 245/477 (51.36%), Postives = 332/477 (69.60%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRH---SKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG-----PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L + PN+CC+LIYTSGTTG PKGVM+SHDNL WTA + Y++ S+EM++SYLPLSH+AAQM DI + + +FAQPDALKGSL +T++EV PT F+GVPRV+EK+ E+MK + S+V++ ++ WAK +G +TNL+KM +NG P Y +AK ++F KV KALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE +GP TI + FR SVGK++P K+++ D + GEIC +GRHVFMGY+N +KT++ D+E WLHSGD+G +D G+LFITGRIKEL+ITAGGENI PVPIE +KE + +SNAM++GDKRK+L +LLTIKC ++ T P D+L P A +G N T +S+I ++P + +I EGI R NE A S + +QKWT L DFS+PGGELGPT+KLKR VV ++Y + + Y+
Sbjct: 265 WAEFMELGRDEPDTKLDDIIAIQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAFAVGRYVRLAEATKSQEMVVSYLPLSHIAAQMVDIWVTMKVGGATYFAQPDALKGSLVNTMREVRPTAFMGVPRVWEKMQEKMKSVGAKSSTVRRKVAVWAKGVGLQTNLSKM-NQNGAMARTPMNYRLAKKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLDIPVYELYGMSESTGPHTISLPDAFRLTSVGKLIPGCETKINNPDEEGNGEICFWGRHVFMGYLNQADKTEDALDAEGWLHSGDLGKHDDNGFLFITGRIKELIITAGGENIPPVPIEDAVKEAVPLVSNAMLIGDKRKFLAMLLTIKCQVNGDTGAPEDELMPEAIELCRKLGSNATRVSEIAGGRDPVIHAAIQEGINRVNEKATSNAQRIQKWTILNQDFSIPGGELGPTMKLKRPVVMKMYKEQVENFYK 740 HSP 2 Score: 120.168 bits (300), Expect = 1.400e-170 Identity = 62/165 (37.58%), Postives = 96/165 (58.18%), Query Frame = 2
Query: 170 VETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
V +++++S D KLRM G A P+T+ MF A+++ + AL +K +++ +INY +YYQ C AAKS +++GL V I G NS EWFI+ I AGG A GIY TN+ + +++ NC+ + +V N + L+K + V P+LKAI+ Y
Sbjct: 90 VAKPDLWTSSGDGEVKLRMGESG-FASEPPLTVDQMFKTAVERFGSYTALGWKEGEQTK--TINYQEYYQACRTAAKSFLKLGLERYHGVGILGFNSAEWFISDIAAILAGGFAVGIYTTNSPDACQYVAENCKANIIVVENHKQLQKILQVQDKLPHLKAIIQY 251
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Match: ENSAMXT00000016625.2 (long-chain-fatty-acid--CoA ligase ACSBG2-like [Source:NCBI gene;Acc:103043523]) HSP 1 Score: 503.827 bits (1296), Expect = 1.794e-176 Identity = 244/471 (51.80%), Postives = 324/471 (68.79%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENG-PFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLD--SEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W +FM LG +I DD L +N NECCSLIYTSGTTGNPKGVM+SHDN+ W A + + +E L+SYLPLSHVAAQ+ D+ + + T HFA+PDALKGSL +TL++V PT F+GVPRV+EK+ E M+ + S++KK I+ WAK IG +TN M + P+G+ +A +IF +V ALG DRC F+ AAP++ + L YF SL++ + E+YGMSE +GP T+ ++N FR S GK++ KLD EDG GE+C +GRHVFMGY+N PEKT E D+E WLHSGD+G + +++ITGRIKEL+ITAGGEN+ PVPIE +KE + ISNAM+VGDK+K+L +LLT+KC +D PTD+LTP A + G T +S+II +K P + +I EG+ NE A S + VQKWT LP DFS+ GGELGPT+KLKR VV+++Y I+ YE
Sbjct: 298 WADFMKLGTDIADDQLDDLINSQNANECCSLIYTSGTTGNPKGVMLSHDNITWIANASGMMIGTKMKEESLVSYLPLSHVAAQVNDLWVSMKFAGTTHFAEPDALKGSLANTLRDVRPTSFLGVPRVWEKMQEAMQSIGAKNSAMKKRIADWAKGIGLQTNYNLMNGDTSVPWGFMLANNLIFKRVRAALGLDRCRTCFTGAAPITKETLEYFMSLNIQLYELYGMSESTGPHTLSWENNFRIMSCGKVIRGCKTKLDKPDEDGN-GEVCFWGRHVFMGYLNMPEKTDEALDTEGWLHSGDLGKLDQNDFMYITGRIKELIITAGGENVPPVPIEDALKEEVSIISNAMLVGDKKKFLAMLLTLKCKVDDNGD-PTDELTPLAVEYCQQRGVTATKVSEIINNKEPAIYKAIQEGVNIINEKATSNAQRVQKWTLLPRDFSISGGELGPTMKLKRPVVSKMYKDDIDKFYE 766 HSP 2 Score: 137.117 bits (344), Expect = 1.794e-176 Identity = 73/183 (39.89%), Postives = 109/183 (59.56%), Query Frame = 2
Query: 140 ITFEIKQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSI 688
ITFE++ S E +S++ DK KLRM G + P+T+H MF + ++K + AL++K + ++ ++ Y QYYQ C AAKS +++GL V I G NSPEWFI+ I AGG+ +GIY TN+ E +++ +NCE V +V N + L K + V P+LKAIV Y G ++ I
Sbjct: 116 ITFEVRLAS---AEQYWSSARDKAVKLRMAESGPGSEA-PLTIHQMFQDTVNKYGDHPALRWK--ENGAWTTLTYKQYYQHCRTAAKSFLKLGLERFHGVGILGFNSPEWFISNVACIMAGGLTTGIYTTNSAEACQYVAHNCEANVIVVENNKQLVKILQVKDQLPHLKAIVQYKGELERKI 292
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:103045839]) HSP 1 Score: 498.819 bits (1283), Expect = 1.937e-170 Identity = 245/477 (51.36%), Postives = 326/477 (68.34%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRH---SKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFG-----YSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EF+ LG N D L ++ PN+CC+LIYTSGTTG PKGVM+SHDNL WTA + + S+E +ISYLPLSH+AAQM DI I + +FAQPDALKGSL +TL+EV PT F+GVPRV+EK+ E+MK + S+V++ ++SWAKD+G TNL+KM ++NG Y +AK ++F KV KALG DRC F+ AAP++ + L +F SLDL + E+YGMSE +GP TI N FR S GK++P KL S D + GEIC +GRHVFMGY+N EKT E D++ WLHSGD+G + + +LFITGRIKEL+ITAGGENI PVPIE +KE + ISNAM++GDKRK+L +LLTIK ++ T P D+LTP A + +G N T +S++ ++ + +I EGI + N+ A S + +QK+T L DFS+ GGELGPT+KLKR VV ++Y I + Y+
Sbjct: 263 WEEFLELGRNEPDTKLNDIIDSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAFATARNVNLDYADQSQEEVISYLPLSHIAAQMIDIWITMQVGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLHTNLSKMEQQNGSMARASLNYRLAKRLVFRKVRKALGLDRCRLCFTGAAPITKETLEFFLSLDLPVYELYGMSESTGPHTISLPNAFRLTSCGKVIPGCQTKLFSPDAEGNGEICFWGRHVFMGYLNMTEKTAEALDADGWLHSGDLGKHDKDDFLFITGRIKELIITAGGENIPPVPIEDAVKEVVPLISNAMLIGDKRKFLSMLLTIKSEVNAETGAPEDELTPEAVEFCRKLGSNATRVSEVAGGRDHLVQAAIQEGINKVNKKATSNAQRIQKFTVLEQDFSITGGELGPTMKLKRPVVMKMYKEQIENFYK 739 HSP 2 Score: 122.094 bits (305), Expect = 1.937e-170 Identity = 61/160 (38.12%), Postives = 93/160 (58.13%), Query Frame = 2
Query: 185 MFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYY 664
+++T D KLRM G A +T+H F A+ + N AL +K ++ + I+Y +Y+ C +AAKS +++GL V I G NSPEWFI+ G IFAGG A GIY TN+ E +++ NC+ + +V N + L+K + + P+LKAI+ Y
Sbjct: 93 LWTTQRDGEVKLRMGESGPAAETA-VTVHQAFEKAVKRFGNYTALGWKEGEQ--WKKISYKEYFDTCLIAAKSFLKLGLERCHGVGILGFNSPEWFISDIGAIFAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQY 249
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Match: acsbg1 (acyl-CoA synthetase bubblegum family member 1 [Source:NCBI gene;Acc:103040425]) HSP 1 Score: 438.343 bits (1126), Expect = 9.249e-148 Identity = 215/472 (45.55%), Postives = 305/472 (64.62%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSED-GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W +FM LG +I D+TL G + PN+CC LIYTSGTTG PKGVM+SHDN+ WTA + + +E ++SYLPLSH+AAQ+ D+ + ++FA+PDALKG+L HTLKE P+ +GVPRV+EK+ME++K + +K+ + +WA + NL E PF + + ++ K+ LGF C FS AAP+ D +F L++ + E YGMSE SGP + ++ S GK++P CK+ +ED G GE+C++GR++FMGY+N +KT E FD + WL SGD+G +++G+LFITGR+KEL+ITAGGEN+ PVPIE +KE L +S+AM++GDKRK+L +LLT+KCV + T PTDDL+P A ++ +G T +S I K+ ++ +I EGI R N A S + +QKWT L DFSV GGELGPT+KL+R ++Y I + Y
Sbjct: 228 WEQFMQLGQDIPDETLDGIIKSQKPNQCCVLIYTSGTTGLPKGVMLSHDNITWTACHASRAGDMQPAEIKQETIVSYLPLSHIAAQIYDMWTGIQWGEMVYFARPDALKGTLVHTLKEATPSSHMGVPRVWEKMMEKIKHELSRAAYLKRKLVNWAMSVTLDANLTYGKDEEKPFLFGVVNSLVIEKLRAELGFSNCEKFFSGAAPIGWDTYKFFLGLNIHLYEAYGMSESSGPHFMSGPQTYKMKSCGKVVPGCRCKIANEDAGGSGEVCLWGRNIFMGYLNMKDKTVEAFDEDGWLCSGDLGKLDEDGFLFITGRLKELIITAGGENVPPVPIEDAVKEELPIVSHAMLIGDKRKFLSMLLTVKCVFNLDTMEPTDDLSPEAVKFCQRLGSQATKVSDITGGKDKLVNHAIQEGIARVNARATSNAQRIQKWTILGKDFSVSGGELGPTMKLRRAAALKMYHNEIEAFYR 699 HSP 2 Score: 107.071 bits (266), Expect = 9.249e-148 Identity = 62/172 (36.05%), Postives = 91/172 (52.91%), Query Frame = 2
Query: 155 KQHSPVETENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
K+ S + +++ T KLR++ + +PIT+H MF N + K AL K E +I++ QYY C M AKS +++GL SV I G NS EWF + G +FAGG A+GIY TN+ E I ++ + +V + L K + + N P+LKAIV G
Sbjct: 50 KKRSDEDARSLWITEASGSVKLRID---EHCPQEPITVHQMFINCVKKYGKLPALATK--TEDKLTTISFCQYYDRCRMTAKSFLKLGLERFHSVAILGSNSEEWFFSAVGAVFAGGFATGIYSTNSPEACHHIASDSRANIIVVDSNWQLDKILLIKDNLPHLKAIVQISG 216
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Match: acsl1a (long-chain-fatty-acid--CoA ligase 1 [Source:NCBI gene;Acc:103038692]) HSP 1 Score: 166.392 bits (420), Expect = 1.165e-42 Identity = 143/482 (29.67%), Postives = 223/482 (46.27%), Query Frame = 1
Query: 784 ININDDTLIGRLNHLIP-----NECCSLIYTSGTTGNPKGVMMSHDNLVWTA---------ECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIF-SAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
I++ + G+ NH P + + +TSGTTGNPKG M++H N++ CML H ++ ISYLPL+H+ ++ + +I L+ A I + Q D L LK + PT F VPR+ ++ +++ F + W D R +++ + S+ +IF+KV +LG C+ + + AAP+S VL + A+L E YG +EC+ ++ ++ G VG LP KL D D EGE+C+ G +VF GY+ PEKT E D + WLH+GD+ + G L I R K + A GE IAP IE ++ D ++ V GD + C++ V +P D P W G S + + K K +I +++ ++ E + +V+ P FSV G L PTLK KR + + I+ LY
Sbjct: 245 ISLKELEATGKANHKRPMPPKPEDLALICFTSGTTGNPKGAMLTHGNVISNCAGFIRITEIHCML------HHDDIHISYLPLAHMFERVVECVI-LVHGARIGYFQGDIR--MLMDDLKTLKPTVFPVVPRLLNRMFDKI------FGQANTPLKRWLLDFATRRKESEL-RSGVVRKDSMWDKLIFSKVQASLG--GCVRLMITGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSMSMPGDWTAGHVGAPLPCNYVKLVDVTDMNYRAASGEGEVCVKGPNVFQGYLKDPEKTAEAIDKDGWLHTGDIAKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYIQS-DPVAQIFVHGDSLQ--------ACLVGIV--VPDPDFLPG---WAKNRGLE-GSYTDLCKSKELKNAI-LEDLVRLGKEAGLKSFEQVRDIVLQPEMFSVQNGLLTPTLKAKRTELRNYFREHIDQLY 692
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Match: acsl1a (long-chain-fatty-acid--CoA ligase 1 [Source:NCBI gene;Acc:103038692]) HSP 1 Score: 165.236 bits (417), Expect = 2.516e-42 Identity = 137/455 (30.11%), Postives = 216/455 (47.47%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYM--KFR-HSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIF-SAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
P + + +TSGTTGNPKG M++H N++ + K R + ++ LIS+LPL+H+ ++ + +I L+ A I + Q D L LK + PT F VPR+ ++ +++ F + W D R +++ + S+ +IF+KV +LG C+ + + AAP+S VL + A+L E YG +EC+ ++ ++ G VG LP KL D D EGE+C+ G +VF GY+ PEKT E D + WLH+GD+ + G L I R K + A GE IAP IE ++ D ++ V GD + C++ V +P D P W G S + + K K +I +++ ++ E + +V+ P FSV G L PTLK KR + + I+ LY
Sbjct: 255 PEDLALICFTSGTTGNPKGAMLTHGNVISNCAGFIRITEGKLRPNRRDTLISFLPLAHMFERVVECVI-LVHGARIGYFQGDIR--MLMDDLKTLKPTVFPVVPRLLNRMFDKI------FGQANTPLKRWLLDFATRRKESEL-RSGVVRKDSMWDKLIFSKVQASLG--GCVRLMITGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSMSMPGDWTAGHVGAPLPCNYVKLVDVTDMNYRAASGEGEVCVKGPNVFQGYLKDPEKTAEAIDKDGWLHTGDIAKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYIQS-DPVAQIFVHGDSLQ--------ACLVGIV--VPDPDFLPG---WAKNRGLE-GSYTDLCKSKELKNAI-LEDLVRLGKEAGLKSFEQVRDIVLQPEMFSVQNGLLTPTLKAKRTELRNYFREHIDQLY 681
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Sea Lamprey
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:ZFIN;Acc:ZDB-GENE-030131-7099]) HSP 1 Score: 489.574 bits (1259), Expect = 9.398e-165 Identity = 244/477 (51.15%), Postives = 319/477 (66.88%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS----KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE--NGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCK--LDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM G + D L + N+CCSLIYTSGTTG+PKGVM+SHDNL WTA + R + +E++ISYLPLSH+AAQ+ DI +P+ +FAQPDALKGSL T+KEV PT F+GVPRV+EK+ME+MK A K+ I+ WAK +G +T+LA M P GY++A ++F KV +ALG DRC ++ AAP++ D L +F SLD+ + E+YGMSE SGP TI F+ GS G +L GC+ L S+D + GE+C +GRHVFMGY+N EKT+E D + WLHSGD+G +++G L+ITGRIKEL+ITAGGEN+ P+PIE IKE L +SNAMV+GD+RK+L VLLT+KC + + P D LT W +G T SQI K+P + +I +GI+R N+ A S + VQKW L DFS+PGGELGPT+KLKR VV ++Y LI LYE
Sbjct: 227 WAEFMRAGEEVPDAQLDAIIASQRANQCCSLIYTSGTTGSPKGVMLSHDNLTWTATVTGELTQLRSADDNYQEVVISYLPLSHIAAQLVDIWLPMRFGGVTYFAQPDALKGSLVVTMKEVRPTGFMGVPRVWEKMMEKMKATAAKSGIFKRKIAGWAKSVGLQTHLANMKGNPAEAPMGYTLANMLVFKKVREALGLDRCTKCYTGAAPITRDTLEFFLSLDIPVFELYGMSESSGPHTISSPTAFQIGSCGTLL--RGCRTLLHSKDEEGSGEVCFWGRHVFMGYLNMEEKTREAIDDDGWLHSGDLGRVDEQGLLYITGRIKELIITAGGENVPPIPIEDAIKEELPVVSNAMVIGDRRKFLSVLLTLKCESNGDSGEPDDALTDEVVHWCQRVGSTATRASQIAGGKDPVVERAIRDGIERVNKRAPSNAQRVQKWELLLRDFSIPGGELGPTMKLKRPVVTKIYQELIERLYE 701 HSP 2 Score: 109.768 bits (273), Expect = 9.398e-165 Identity = 59/164 (35.98%), Postives = 93/164 (56.71%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
+ +++T D LR+N G L+ P T++ +F + + +AL K + +++ + Y +YY C AAKS +++GL V I G NS EWFI+ G I AGG A GIY TN+ E +++ +NC + +V N++ L+K + V P+LKAIV Y G
Sbjct: 55 DRLWTTERDGAVLLRINASGPLSDT-PETINQLFERTVKLYGDHKALASK--EGNTWKILTYKEYYAQCRQAAKSFLKLGLQRYHGVGILGFNSAEWFISDIGAILAGGFAVGIYTTNSAEACQYVADNCLANIIVVENDKQLQKILEVQSQLPHLKAIVQYKG 215
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Sea Lamprey
Match: ACSL1 (acyl-CoA synthetase long chain family member 1 [Source:HGNC Symbol;Acc:HGNC:3569]) HSP 1 Score: 163.31 bits (412), Expect = 3.228e-42 Identity = 140/476 (29.41%), Postives = 231/476 (48.53%), Query Frame = 1
Query: 805 LIGRLNHL-----IPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK---FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQA-TIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM----MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFS--GIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHD-FSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
+IG+ NH P++ + YTSGTTGNPKG M++H N++ L K ++ ++LIS+LPL+H+ ++ + I LC + F Q D L +K + PT F VPR+ +I +++ S K W + +A++ ++++ SI +IF++V ++G R + + AAP+S +L + A L + E +G +EC+ T ++ G VG +P K+ D + EGE+C+YG +VF GY+ P +T E D + WLH+GD+G + G L I R K + + GE IAP IE + + ++ V GD + V C++ +P ++ P+ W GI NP S + +K+ + +I D + R + A +S E + L D FS+ G L PT K+KR V + + + I+SLY
Sbjct: 255 VIGKTNHREPIPPQPDDIAIICYTSGTTGNPKGAMLTHKNMISDFSAFLAITKDTFLPNTDDVLISFLPLAHMFERLVEASI--LCNGGRVGFYQGDIR--LLMDDMKVLQPTVFPVVPRLLNRIHDKV------LSGAKSHFKRWLLEFAVSRKIAELRCGVVRKD-----SIWDKLIFHRVQASMG-GRVRFMVTGAAPISASILTFLRAILGCQVYEGFGQTECTAGCTFTMPSDSTAGHVGPPMPCNHIKVVDVAEMNYFAANGEGEVCVYGTNVFKGYLKDPTRTAEALDEDGWLHTGDIGKWLPNGTLKIVDRKKHIFKLSQGEYIAPEKIE-TVYVRSEPVAQVFVHGDSLQSCLV-----CIV-----VPDMEVLPS---WVQKRGIKCNPNS---VFINKDVRAAILHD--MVRLGKEAGLKSFEQVRAVHLHSDLFSIENGLLTPTFKVKRAEVCKFFHSEIDSLY 695
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Sea Lamprey
Match: ACSL1 (acyl-CoA synthetase long chain family member 1 [Source:HGNC Symbol;Acc:HGNC:3569]) HSP 1 Score: 143.665 bits (361), Expect = 4.683e-37 Identity = 127/461 (27.55%), Postives = 219/461 (47.51%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK---FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQ-ATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKM----MKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSEDGK------EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHD-FSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
P + + +TSGTTGNPKG M++H N+V L + + ++ ISYLPL+H+ ++ + L C A+I F Q D L ++ + PT F VPR+ ++ +++ S + + G R +A++ ++ N S+ ++F + ++G R I + AAP+S VL + + + E YG +EC+ T+ +++ G VG +P KL D +D + +GE+C+ G +VF GY+ EKT E D + WLH+GD+G + G L I R K + A GE IAP IE + + ++ V GD + C++ V P K+ G + S++ +N ++ +I E ++R + + + E K L ++ FS+ G L PT K KR + + ++I+S+Y+
Sbjct: 18 PEDLAIVCFTSGTTGNPKGAMLTHRNIVSDMSGFLKVTESLFLPETSDIAISYLPLAHMFERL--VQATLFCHGASIGFFQGDVR--LLLDDMQALRPTVFPVVPRILNRMYDKV------LGSTRTPFRRKLLEFGARRKMAELQRGVVRRN-----SLWDWLVFRPMQLSVG-GRVRMIMTGAAPISPGVLNFLRVVMGCQMYEGYGQTECTAGCTLTLPGDWKAGHVGAPMPCNYIKLHDVKDMEYYTSQGKGEVCVKGPNVFKGYLKDAEKTAEAVDRDGWLHTGDIGQWMPNGTLKIIDRKKHIFKLAQGEYIAPEKIEN-VYSRCEPVAQVYVHGDSLQ--------ACLVAVVVPDPEILCCWIRKKGIVG------TYSELC--RNKEVRQAILEDMQRLGKESDLQPFEQVKDVHLHNEMFSIENGLLTPTFKAKRTELRTHFRSVISSMYQ 445
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Sea Lamprey
Match: acsl3b (acyl-CoA synthetase long chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]) HSP 1 Score: 66.6254 bits (161), Expect = 3.741e-12 Identity = 71/253 (28.06%), Postives = 114/253 (45.06%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHV---AAQMCDIIIPLLCQATIHFAQ------PDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVIC----EIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEG 1551
P + ++YTSG+TG PKGV ++H NLV M + + I YLPL+HV A+M + + C+ AQ KGS G + + PT VP + E+I + + S +++ I A Y L ++ E G F + ++FNK+ LG + L + S AAPLS + +++ C + YG++E TI ++ G VG P C++ + +EG
Sbjct: 59 PRDLAVVMYTSGSTGIPKGVRIAHSNLVAAITGMYLRINDICGDDTYIGYLPLAHVLELGAEM--VCLSRGCRIGYSSAQTLTDQSTRIKKGSQGD-VTILRPTLMAAVPEIMERIYKGVMGKVQCMSLLQRIIFKLA----YNYKLEQL--ERG-FDTPLCNRLVFNKLCALLGGNVRL-LLSGAAPLSPRTQRF---MNVCFCCPVGQGYGLTETCAAGTIAELQDYSTGHVGA--PLSCCEIQLRNWEEG 295
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Match: FAA2 (Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon [Source:SGD;Acc:S000000817]) HSP 1 Score: 117.857 bits (294), Expect = 1.064e-27 Identity = 98/351 (27.92%), Postives = 159/351 (45.30%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKF----RHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFA-QPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGP----FGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFAS-LDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS--------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCV 1830
P+ ++ +TSGTTG PKGV MSH N+ + R+ + + +LPL+H+ +M I L I F +PD L LK + P VPR+ + +K A+D S+V++++++ D + A+ GP + + V+ +K+ +LG I + +AP+S D L++ S LD+ I + YG++E + E GS G I C+L S + +GE+ + G VF Y P +T + D + W +GDV + +G + + R+K A GE IAP IE + +I+ V GD K V
Sbjct: 291 PDSLYTISFTSGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIFERMV-IAYDLAIGFGIGFLHKPDPT--VLVEDLKILKPYAVALVPRILTRFEAGIKN-ALDKSTVQRNVANTILD----SKSARFTARGGPDKSIMNFLVYHRVLIDKIRDSLGLSNNSFIITGSAPISKDTLLFLRSALDIGIRQGYGLTETFAGVCLSEPFEKDVGSCGAIGISAECRLKSVPEMGYHADKDLKGELQIRGPQVFERYFKNPNETSKAVDQDGWFSTGDVAFIDGKGRISVIDRVKNFFKLAHGEYIAPEKIENIYLSSCPYITQIFVFGDPLKTFLV 633
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Match: FAA1 (Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 [Source:SGD;Acc:S000005844]) HSP 1 Score: 113.235 bits (282), Expect = 2.716e-26 Identity = 93/340 (27.35%), Postives = 159/340 (46.76%), Query Frame = 1
Query: 835 NECCSLIYTSGTTGNPKGVMMSHDNLVW-TAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHT----LKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRK 1818
++ C ++YTSG+TG PKGV++ H N+V L +KF + + +I +LPL+H+ + +++ A I +A L S L+E PT +GV V+E + + + + + K I W Y T L M + + P G ++ ++F K+ A G + + + +P+S D + +L + YG++E TI F G G + KL D E+ +GE+ + G +V Y E+T + S+ W +GD+G + G+L I R K L+ T GE IA +E + N ++++N V D+ K
Sbjct: 262 DDLCCIMYTSGSTGEPKGVVLKHSNVVAGVGGASLNVLKFVGNTDRVICFLPLAHIFELVFELL-SFYWGACIGYATVKTLTSSSVRNCQGDLQEFKPTIMVGVAAVWETVRKGILNQIDNLPFLTKKIF-WT---AYNTKL-NMQRLHIPGGGALGN-LVFKKIRTATG-GQLRYLLNGGSPISRDAQEFITNLICPMLIGYGLTETCASTTILDPANFELGVAGDLTGCVTVKLVDVEELGYFAKNNQGEVWITGANVTPEYYKNEEETSQALTSDGWFKTGDIGEWEANGHLKIIDRKKNLVKTMNGEYIALEKLESVYRSN-EYVANICVYADQSK 592
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Match: FAA4 (Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 [Source:SGD;Acc:S000004860]) HSP 1 Score: 88.9669 bits (219), Expect = 1.138e-20 Identity = 89/393 (22.65%), Postives = 171/393 (43.51%), Query Frame = 1
Query: 766 EFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLY-YMKFRHSKEMLISYLPLSHVAAQMCDIII----PLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTI-------KCVIDPVTQIPTDDL 1887
E + +G D+ L+ P + ++YTSG+TG PKGV+++H N+V + + + + + +I++LPL+H+ + +L A + P + + G L E PT +GV V+E + + + + + + + W++ + + +KE + +IF ++ +A G + I + + +S D + ++L + YG++E + F +G G ++ KL D ED +GE+ G + Y PE+T F + W +GD+ + +G + I R K L+ T GE IA +E + N ++ N V D+ K V + + K I+ +P +D+
Sbjct: 242 EIIEIGKKAKDEV---ELHFPKPEDPACIMYTSGSTGTPKGVVLTHYNIVAGIGGVGHNVIGWIGPTDRIIAFLPLAHIFELTFEFEAFYWNGILGYANVKTLTPTSTRNCQGD-LMEFKPTVMVGVAAVWETVRKGI------LAKINE-LPGWSQTLFWTV---YALKERNIPCSGLLSGLIFKRIREATGGNLRF-ILNGGSAISIDAQKFLSNLLCPMLIGYGLTEGVANACVLEPEHFDYGIAGDLVGTITAKLVDVEDLGYFAKNNQGELLFKGAPICSEYYKNPEETAAAFTDDGWFRTGDIAEWTPKGQVKIIDRKKNLVKTLNGEYIALEKLESIYRSN-PYVQNICVYADENKVKPVGIVVPNLGHLSKLAIELGIMVPGEDV 618 HSP 2 Score: 28.8758 bits (63), Expect = 1.138e-20 Identity = 26/117 (22.22%), Postives = 51/117 (43.59%), Query Frame = 2
Query: 323 YKYSQESSFLSINYSQYYQLCCM--AAKSLIQIGL--NARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVY 661
+ Y + + ++++ Y + +C M + LI+IG+ N I S +W T+ G + G Y T + + + + VAI + +LL K K N+K +++
Sbjct: 89 WLYYELTPYITMTYEE--MICVMHDIGRGLIKIGVKPNGENKFHIFASTSHKWMKTFLGCMSQGIPVVTAYDTLGESGLIHSMVETDS-VAIFTDNQLLSKLAVPLKTAKNVKFVIH 202
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Match: FAA3 (Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery [Source:SGD;Acc:S000001271]) HSP 1 Score: 88.1965 bits (217), Expect = 1.975e-18 Identity = 91/347 (26.22%), Postives = 151/347 (43.52%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLV-WTAECMLYYMKFRHSKEMLISYLPLSHV-------AAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEI---YGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRK 1818
P + ++YTSG+ PKGV+++H N+V A + S + ++S+LPL+H+ A + I+ T+ KG L E PT IGV V+E + + + E D + V + I W+ A MKE ++F KV +A G I + + +S D +F+ +V+C + YG++E + + F +G VG ++ KL D +D +GE+ + G V Y P +T F + W +GD+ + +G L I R K L+ T GE IA +E + N ++ N V D+ +
Sbjct: 261 PKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTRNCKGDL----VEFKPTIMIGVAAVWETVRKAILEKISDLTPVLQKIF-WS---------AYSMKEKSVPCTGFLSRMVFKKVRQATG-GHLKYIMNGGSAISIDAQKFFS---IVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYYKNPIETAVSFTYDGWFRTGDIVEWTPKGQLKIIDRRKNLVKTLNGEYIALEKLESVYRSN-SYVKNICVYADESR 588
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Match: PCS60 (Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase [Source:SGD;Acc:S000000426]) HSP 1 Score: 71.633 bits (174), Expect = 1.780e-13 Identity = 46/122 (37.70%), Postives = 66/122 (54.10%), Query Frame = 1
Query: 1414 ICEIYGMSECSGPQTIEF--QNEFRFGSVGKILPDFGCKLDSED-----GKEGEICMYGRHVFMGYVNAPEKTKEVF-DSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIE 1755
+ E Y M+E S T + + G+VG+ LD D GK GE+ + G +V +GY N P+ KE F E + +GD G F+ EG+L +TGRIKE LI GGE I+P+ ++
Sbjct: 330 VLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKE-LINRGGEKISPIELD 450
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Match: EDO32054 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW65]) HSP 1 Score: 432.565 bits (1111), Expect = 4.194e-143 Identity = 218/447 (48.77%), Postives = 296/447 (66.22%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS--EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELG 2094
W +F+ LG + D L R++ L+ N CC+LIYTSGTTG PKGVM+SHDN+ WTA+ L +++ +E ++SYLPLSHVAAQM DI + + C T+ FAQPDALKGSL +TL+E PT F+GVPRV+EKIME+M E+ + K+ I+ WAK + N+ + PFG++IA I K+ +LG DRC G F AAAP++ + L YF S+++ + E+YGMSECSGP TI GS G + K+D EDG GE+CM GR+VFMG++N EKTKE D + WLHSGDV +D G +L+ITAGGENIAPVPIE IK+ + ISNA+++GDKRK+L LLT+K ++ T PTD LT AA + +G T++S+I+ K+ + +I G+ R+NE AISR+ +VQKW L +DF++ GGELG
Sbjct: 113 WNQFLELGKTVADRVLQERMSQLVANRCCTLIYTSGTTGAPKGVMISHDNITWTAQAALNHVQAGKREEHIVSYLPLSHVAAQMTDIYMSMYCCGTVWFAQPDALKGSLVNTLRECRPTLFLGVPRVWEKIMEKMLEVGATVTGPKRRIADWAKGKALQGNMNIEQGRSTPFGWTIATG-ILKKIKVSLGLDRCWGQFVAAAPVTMETLRYFQSINIPLYELYGMSECSGPLTISIPGHVLSGSCGIKMEGTEMKIDKPDEDGN-GELCMRGRNVFMGFLNNEEKTKEALDVDGWLHSGDVCKIDDVSIRIAEG---QLIITAGGENIAPVPIEDNIKKEVPLISNAILIGDKRKFLSCLLTLKVEVEADTGAPTDRLTKAAIAFCKSVGSEATTVSEILKTKDENVMKAIQAGVDRANEEAISRAQKVQKWCILENDFTIHGGELG 554 HSP 2 Score: 55.8398 bits (133), Expect = 5.179e-8 Identity = 37/108 (34.26%), Postives = 51/108 (47.22%), Query Frame = 2
Query: 362 YSQYYQLCCMAAKSLIQ--IGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLID 679
YS+YY+ +AAKS I+ G N R I G A GIY TN+ E F+ +NC V +V N+ L K + V P+LKA+V Y G ++
Sbjct: 14 YSEYYEEVRIAAKSFIEREWGRNGR-----------------LHCILIRGFAVGIYTTNSPEACHFVADNCNANVIVVENKAQLSKILKVWDRLPHLKAVVQYTGEVE 104
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Match: EDO45461 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSR3]) HSP 1 Score: 153.68 bits (387), Expect = 9.575e-43 Identity = 136/469 (29.00%), Postives = 210/469 (44.78%), Query Frame = 1
Query: 808 IGRLNHLI-----PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPR----VFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF--ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILP-------DFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
IGR N + PN+ ++ YTSGTTG PKG + +H N++ ++ ++ SYLPL+H A + +I L I F + D L ++E+ PT+F VPR V++K+M Q+ E SW G A M K N +I V+F KV G R I S AAPLS V+ +F A D I E YG +EC+ +++ + + G VG L D K S +GE+C+ G +V Y PEKT E D + WLH+GD+G + + G L I R K++ A GE +AP +E I + F++ A V G+ K V + +P ++ K+W + N + + +K ++ ++ E + +V+ P FSV + T K+KR + + + +I SLY
Sbjct: 224 IGRKNIKVVNPPKPNDIFTICYTSGTTGTPKGAVQTHSNVIADVSGVIRQYN-TCGIDVHFSYLPLAH-AYERTGVICMLQSGCRIGFYRGDP--QLLFEDIQELKPTFFASVPRIMNKVYDKVMAQVSE---------SKFKSWMFQKGLHAKKADMNK-NVFRSNTIWDKVVFKKVQMLFG-GRVRAITSGAAPLSPKVMTFFRCAMGDCRIMEGYGQTECTACASLQLPQDMQAGIVGPPLTCNYIKLVDVPEKSYSTKDLKGEVCIKGPNVIPRYHKNPEKTAETLDEDGWLHTGDIGMWLENGALKIIDRKKDIFKLAQGEYVAPGKLEN-IAQRSPFVAQAFVHGESLKTFVVAIL----------VPDGEVL---KKWAA--QKNIQGDLKTLCEKEEVRQAVFEDVVRVCKEAELFSFEQVKAVHLHPEAFSVENDLMTATFKIKRPQIIKAFHDVIESLY 661 HSP 2 Score: 39.6614 bits (91), Expect = 9.575e-43 Identity = 32/129 (24.81%), Postives = 58/129 (44.96%), Query Frame = 2
Query: 356 INYSQYYQLCCMAAKSLIQIGLNA--RKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDVYVFLI 736
++Y++ + +L+ +G + R V I N EW +T V+ IY T+ + FIIN+ + + IV N + + K P+LK I+ G L+ + + K+S ++ F I
Sbjct: 93 LSYNEVRERAHSFGSALVHLGCHPSQRTFVGIQAKNCVEWVLTDLACQMFSMVSVPIYDTHGPDACLFIINHAD-VETIVCNHTKVPFILKNIKRIPHLKCIIQIGELVKEEERKDAKVSGIELLSFSI 220
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Match: EDO46361 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQG4]) HSP 1 Score: 156.762 bits (395), Expect = 1.589e-40 Identity = 137/476 (28.78%), Postives = 225/476 (47.27%), Query Frame = 1
Query: 784 ININDDTLIGRLNHLIP--NECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK-----FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFAS-LDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAG-GENIAPVPIEQKIKENLDFISNAMVVGDK-RKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
I+ +D + RL P ++ ++ YTSGTTG PKG M++HDN++ LY +K + ++ +S+LPLSH+ ++ I+I + I F D K L K + PT F VPR+ +I +++ I+ S +K+ I A + R K+++ + + Y +IFNK+ LG R I S +APLS V + + + E YG +E T++ ++F G VG P KL + K+GEIC+ GR+VF GY++ P T EV D + WL++GD+G + G + R + I GE IAP IE I F++ V GD R +L ++ +P ++ A W G + ++ +K I + +K E+ ++ +V+ FS+ G L PTLK+KR ++ + +I++LY
Sbjct: 92 ISSSDRSAAARLRIKPPKSDDLYTVCYTSGTTGTPKGAMLTHDNVISDLSAFLYVLKQVIIRYFGVDDVHLSFLPLSHMYERVNQIVI-FMNGGKIGFFNGDIKK--LLDDFKTLRPTVFGAVPRLLNRIHDKVTN-EINKSWIKRWIFQIALNAKKRDLARKIVRRDTIWDY-----LIFNKIQALLG-GRVWFIISGSAPLSPKVATFIRCVMGCQVQEGYGQTETVASVTLQLLDDFTAGHVGPPTPVNVVKLVDVPEMDYWANNKQGEICVKGRNVFKGYLHNPAMTAEVLDKDRWLYTGDIGEWTTVGTRRLYTRERGACIQGNEGEYIAPQKIEN-IYLRSPFVAQIYVHGDSIRSFLVAVV-----------VPDAEVLEA---WAEKKGIR-GDLKRLCQNKTVHQRI-FESMVKVGEESQLNSLEKVRGIHLCSEAFSIDNGLLTPTLKIKRPQMSTFFRDIIDNLY 540
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Match: EDO46335 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQC0]) HSP 1 Score: 157.918 bits (398), Expect = 2.005e-40 Identity = 137/463 (29.59%), Postives = 209/463 (45.14%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK----FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQ-MKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLD--------SEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITD-----KNPKLSISIDEGIKRSNENAISRSAEVQKWTFL-PHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
P++ + YTSGTTGNPKG M++H N V + +K + ++ ISYLPL+H+ ++ ++I + + F + K + L+E+ PT FI VPRV ++ ++ MKE I SS+KK + + A ++++N + Y ++FNK+ + E YG +E + TI+ + FG VG +P KL ++DGK GEIC YG +VF GY++ PEKT+E D + WLHSGDVG + + G L + R K + A GE IAP IE + + V GD RK C++ V T A K+W T +++ D N K+ +I E + + S + K +L P F+V G L PTLK KR V+ + LY+
Sbjct: 254 PDDLAVVCYTSGTTGNPKGAMITHSNAVAMLAGLCVMIKKCGIRVDNDDVHISYLPLAHMYERLAQLMI-FMYGGKVGFFGGNIRK--IMEDLQELKPTVFISVPRVLNRVYDKVMKE--ISSSSIKKFVFNTALSFKKAEVERNIVRKNSIWDY-----LVFNKIQ--------------------------------VLEGYGQTETTAAATIQLVGDHTFGHVGPPMPCTKIKLVDVPDMNYFAKDGK-GEICFYGPNVFKGYLHDPEKTREAIDEDGWLHSGDVGEWTENGTLKLIDRKKHIFKLAQGEYIAPEKIEA-VYMQCPLVHQVFVYGDSRK--------SCIVGVVVPEET-----ALKKW--------TEKNEVQGDMEALCNNEKVRKAILEEMSAMGKKEKLFSFQQVKDIYLHPDPFTVDNGLLTPTLKFKRPVIQAHFKDTFEKLYD 651
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Match: EDO45504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSR2]) HSP 1 Score: 147.517 bits (371), Expect = 4.261e-37 Identity = 131/491 (26.68%), Postives = 232/491 (47.25%), Query Frame = 1
Query: 733 DLIYMIIV*WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEML-----ISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFAS-LDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDS-------EDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRW-FSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
D++ + I+ + E +GIN + + R P + ++ +TSGTTG PKGV+++HDN++ L+ +K + +E+ +SYLP H+ + C ++ ++ A I + L K + PT F VPR+ I +++ + + S +A + + + ++++++ +I ++F K+ +G + + I +APLS V + + + E YG +E + T++ +F G VG +P KL +GEIC+ GR+VF GY++ E+T+E FDS W +SGD+G + +G L I R K + + GE IAP I+ ++ F++ V G+ K V V+ P ++ L P AK G I Q+ ++ + +I D I + E ++ +V+ P F+V G L PTLK KR + + + +N LYE
Sbjct: 167 DILGIRIISFDEVEKIGINNPHEKVPAR-----PEDTFTISWTSGTTGFPKGVILTHDNIISDMSAYLFLIK-QDGEELTPQDTHLSYLPPEHMY-ERCTQVLMMMAGAKIGLYH--GKREFLMDDFKALRPTVFAAVPRLLNAIYDKVVPL--------RRYSLFAGNTFF-FHARRIIRKD-----TIWDRLVFKKIQDLIGGNVRI-ITCGSAPLSARVTSFIRCVMGCYLNEGYGSTETTAMVTMQRLKDFNTGHVGAPVPCATVKLVDVPEMDYYASNNQGEICIRGRNVFKGYLHDAERTEEAFDSSGWFYSGDIGEWTQDGTLRIIDRKKHIFKLSQGEYIAPAKIQNAYLRSM-FVAQVFVHGNSLKSFIV-----AVVVPDAEV----LIPWAKTHDIEG------DIKQLCQNQTVRKAIHGDM-IAKGKEAKLNSLEQVKGIYLHPELFTVEEGLLTPTLKTKRPALAQFFEEKLNVLYE 616
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:101171154]) HSP 1 Score: 498.434 bits (1282), Expect = 2.906e-172 Identity = 240/476 (50.42%), Postives = 331/476 (69.54%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE----NGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L + PN+CCSLIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM D+ + + + HFAQPDALKGSL +TLKEV PT F+GVPRV+EK+ E+MK + S++++ ++SWAKD+G +TNL +M + PF Y +AK ++F KV KALG DRC ++ AAP + D L +F SLD+ + E+YGMSEC+GP TI + FR S GK +P KLD+ D + GEIC +GRH+FMGY+NAP+KT+E D+E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +K+ + +SNAM++GDKRK+L +L+TIKC I+P T P D+LTP A +G + ++S+I ++ + +I EGI + N+ A S + +QK+ L DFS+ GGELGPT+KLKR VV ++Y I++ Y+
Sbjct: 281 WAEFMELGRDEPDGPLDAIIASQKPNQCCSLIYTSGTTGQPKGVMLSHDNLTWTALSTAKHVCLTDATVAQEVVVSYLPLSHIAAQMVDMWLIMRVGGSTHFAQPDALKGSLVNTLKEVRPTAFMGVPRVWEKMQEKMKSIGAKSSTLRRKVASWAKDVGLQTNLTRMRQNTATGQAPFQYHLAKKLVFKKVRKALGLDRCTKCYTGAAPTTKDTLEFFLSLDIPLFELYGMSECTGPHTISRPDAFRLTSCGKEIPGCTTKLDNPDEEGNGEICFWGRHIFMGYLNAPDKTEEALDAEGWLHSGDLGKRDEHGFLYITGRIKELIITAGGENIPPVPIEDAVKDAVPLLSNAMLIGDKRKFLSMLVTIKCQINPETGNPEDELTPEAVEICKKLGTSAKTVSEIAGGRDRTVHAAIQEGINQVNKKATSNAQCIQKFVILNRDFSINGGELGPTMKLKRPVVLKMYKDQIDNFYK 756 HSP 2 Score: 128.257 bits (321), Expect = 2.906e-172 Identity = 64/164 (39.02%), Postives = 97/164 (59.15%), Query Frame = 2
Query: 185 MFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLI 676
++++ D KLRM G +A P+T+H MF +A+++ + AL +K ++ S+NY YY+ C AAKS +++GL VCI G NS EWFI G I AGG G+Y TN+ E +F+ NC+ V ++ NE+ L+K + + P+LKAIV Y G +
Sbjct: 111 LWTSQGDAEVKLRMGESG-VAAEPPLTVHQMFTSAVERYGDNTALAWKEGDQAK--SLNYKDYYKACRTAAKSYLKLGLQRYHGVCILGFNSAEWFIADIGAILAGGFGVGVYTTNSPEACQFVAENCKANVIVLENEKQLQKILQIEDQLPHLKAIVQYKGAL 271
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:NCBI gene;Acc:101171154]) HSP 1 Score: 498.049 bits (1281), Expect = 3.888e-172 Identity = 240/476 (50.42%), Postives = 331/476 (69.54%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHS---KEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE----NGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
W EFM LG + D L + PN+CCSLIYTSGTTG PKGVM+SHDNL WTA ++ + +E+++SYLPLSH+AAQM D+ + + + HFAQPDALKGSL +TLKEV PT F+GVPRV+EK+ E+MK + S++++ ++SWAKD+G +TNL +M + PF Y +AK ++F KV KALG DRC ++ AAP + D L +F SLD+ + E+YGMSEC+GP TI + FR S GK +P KLD+ D + GEIC +GRH+FMGY+NAP+KT+E D+E WLHSGD+G ++ G+L+ITGRIKEL+ITAGGENI PVPIE +K+ + +SNAM++GDKRK+L +L+TIKC I+P T P D+LTP A +G + ++S+I ++ + +I EGI + N+ A S + +QK+ L DFS+ GGELGPT+KLKR VV ++Y I++ Y+
Sbjct: 272 WAEFMELGRDEPDGPLDAIIASQKPNQCCSLIYTSGTTGQPKGVMLSHDNLTWTALSTAKHVCLTDATVAQEVVVSYLPLSHIAAQMVDMWLIMRVGGSTHFAQPDALKGSLVNTLKEVRPTAFMGVPRVWEKMQEKMKSIGAKSSTLRRKVASWAKDVGLQTNLTRMRQNTATGQAPFQYHLAKKLVFKKVRKALGLDRCTKCYTGAAPTTKDTLEFFLSLDIPLFELYGMSECTGPHTISRPDAFRLTSCGKEIPGCTTKLDNPDEEGNGEICFWGRHIFMGYLNAPDKTEEALDAEGWLHSGDLGKRDEHGFLYITGRIKELIITAGGENIPPVPIEDAVKDAVPLLSNAMLIGDKRKFLSMLVTIKCQINPETGNPEDELTPEAVEICKKLGTSAKTVSEIAGGRDRTVHAAIQEGINQVNKKATSNAQCIQKFVILNRDFSINGGELGPTMKLKRPVVLKMYKDQIDNFYK 747 HSP 2 Score: 128.257 bits (321), Expect = 3.888e-172 Identity = 64/164 (39.02%), Postives = 97/164 (59.15%), Query Frame = 2
Query: 185 MFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLI 676
++++ D KLRM G +A P+T+H MF +A+++ + AL +K ++ S+NY YY+ C AAKS +++GL VCI G NS EWFI G I AGG G+Y TN+ E +F+ NC+ V ++ NE+ L+K + + P+LKAIV Y G +
Sbjct: 102 LWTSQGDAEVKLRMGESG-VAAEPPLTVHQMFTSAVERYGDNTALAWKEGDQAK--SLNYKDYYKACRTAAKSYLKLGLQRYHGVCILGFNSAEWFIADIGAILAGGFGVGVYTTNSPEACQFVAENCKANVIVLENEKQLQKILQIEDQLPHLKAIVQYKGAL 262
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Match: ENSORLT00000027775.1 (long-chain-fatty-acid--CoA ligase ACSBG2 [Source:NCBI gene;Acc:101158282]) HSP 1 Score: 473.781 bits (1218), Expect = 1.825e-166 Identity = 235/473 (49.68%), Postives = 321/473 (67.86%), Query Frame = 1
Query: 748 IIV*WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEN-GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
I+ W EFM +G +++D+ L ++ NECC+LIYTSGTTGNPKGVM+S DN+ WTA + + +E+LISYLPLSHVAAQM DI I + T +FA+PDALKGSL +TLKE PT F+GVPRV+EK+ E M+ S +KK ++ WAK IG + N + M EN P+G+ +A ++F KV ALG DRC F+ AAP++ D L YF SL++ + E+YGMSE SGP T+ +E+R GS GK++P KL++ D + GE+C +GR+VFMGY+N P+KT+E D + WLHSGD+G F+ E +L+ITGRIKEL+ITAGGENI PVPIE +K L ISN M++GDK K+L +L+T+KCV+D + P ++L+ + T +S+I +K P + +I EGI R N + S + ++QKW L DFSV GGELGPTLKL+R +V ++Y IN LY
Sbjct: 248 ILYSWEEFMKVGEDVSDEQLNAVIDSQRANECCTLIYTSGTTGNPKGVMLSQDNITWTARISSGLICKNYGQEILISYLPLSHVAAQMVDIWISMSFSGTTYFAEPDALKGSLVNTLKEARPTCFLGVPRVWEKMQEGMRAGGEKASPLKKRVADWAKSIGLQYNYSAMEGENLVPWGFMLANNLVFKKVRAALGLDRCRYCFTGAAPITKDTLEYFLSLNIPVKELYGMSESSGPHTVSI-DEYRIGSCGKVIPGSKIKLNNPDEEGNGEVCFWGRNVFMGYLNMPDKTEEALDEDGWLHSGDLGKFDQENFLYITGRIKELIITAGGENIPPVPIEDAVKSELPIISNTMLIGDKLKFLSMLITLKCVMDDNGE-PLNELSSEVLDFCRRHSVTATKVSEITANKEPAIYKAIQEGIDRVNARSTSNAQKIQKWVVLERDFSVVGGELGPTLKLRRPIVVKMYQEKINELY 718 HSP 2 Score: 133.65 bits (335), Expect = 1.825e-166 Identity = 65/164 (39.63%), Postives = 99/164 (60.37%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
+ ++S S DK LRM G + P+T+H MF +++ + AL +K +++ S+ + QYY+ C AAKS +++GL V I G NSPEWFI+ G IFAGG+A+GIY TN+ E +++ NCE + +V N++ L K + V P+LKAI+ Y G
Sbjct: 80 DKLWSASRDKAVNLRMEASGPGSET-PVTIHQMFLETVERSGDQTALVFKEDGQTA--SLTWRQYYEQCRAAAKSFLKLGLERFHGVGILGFNSPEWFISDIGCIFAGGLAAGIYTTNSPEACQYVAANCEANILVVENQKQLDKILQVKDQLPHLKAIIQYKG 240
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Match: ENSORLT00000001544.2 (long-chain-fatty-acid--CoA ligase ACSBG2 [Source:NCBI gene;Acc:101158282]) HSP 1 Score: 473.781 bits (1218), Expect = 1.825e-166 Identity = 235/473 (49.68%), Postives = 321/473 (67.86%), Query Frame = 1
Query: 748 IIV*WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEN-GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGK-EGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
I+ W EFM +G +++D+ L ++ NECC+LIYTSGTTGNPKGVM+S DN+ WTA + + +E+LISYLPLSHVAAQM DI I + T +FA+PDALKGSL +TLKE PT F+GVPRV+EK+ E M+ S +KK ++ WAK IG + N + M EN P+G+ +A ++F KV ALG DRC F+ AAP++ D L YF SL++ + E+YGMSE SGP T+ +E+R GS GK++P KL++ D + GE+C +GR+VFMGY+N P+KT+E D + WLHSGD+G F+ E +L+ITGRIKEL+ITAGGENI PVPIE +K L ISN M++GDK K+L +L+T+KCV+D + P ++L+ + T +S+I +K P + +I EGI R N + S + ++QKW L DFSV GGELGPTLKL+R +V ++Y IN LY
Sbjct: 248 ILYSWEEFMKVGEDVSDEQLNAVIDSQRANECCTLIYTSGTTGNPKGVMLSQDNITWTARISSGLICKNYGQEILISYLPLSHVAAQMVDIWISMSFSGTTYFAEPDALKGSLVNTLKEARPTCFLGVPRVWEKMQEGMRAGGEKASPLKKRVADWAKSIGLQYNYSAMEGENLVPWGFMLANNLVFKKVRAALGLDRCRYCFTGAAPITKDTLEYFLSLNIPVKELYGMSESSGPHTVSI-DEYRIGSCGKVIPGSKIKLNNPDEEGNGEVCFWGRNVFMGYLNMPDKTEEALDEDGWLHSGDLGKFDQENFLYITGRIKELIITAGGENIPPVPIEDAVKSELPIISNTMLIGDKLKFLSMLITLKCVMDDNGE-PLNELSSEVLDFCRRHSVTATKVSEITANKEPAIYKAIQEGIDRVNARSTSNAQKIQKWVVLERDFSVVGGELGPTLKLRRPIVVKMYQEKINELY 718 HSP 2 Score: 133.65 bits (335), Expect = 1.825e-166 Identity = 65/164 (39.63%), Postives = 99/164 (60.37%), Query Frame = 2
Query: 179 ENMFSTSLDKVSKLRMNCKGDLARIKPITLHTMFHNAIDKGANTEALKYKYSQESSFLSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGG 670
+ ++S S DK LRM G + P+T+H MF +++ + AL +K +++ S+ + QYY+ C AAKS +++GL V I G NSPEWFI+ G IFAGG+A+GIY TN+ E +++ NCE + +V N++ L K + V P+LKAI+ Y G
Sbjct: 80 DKLWSASRDKAVNLRMEASGPGSET-PVTIHQMFLETVERSGDQTALVFKEDGQTA--SLTWRQYYEQCRAAAKSFLKLGLERFHGVGILGFNSPEWFISDIGCIFAGGLAAGIYTTNSPEACQYVAANCEANILVVENQKQLDKILQVKDQLPHLKAIIQYKG 240
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Match: acsl5 (acyl-CoA synthetase long chain family member 5 [Source:NCBI gene;Acc:101166257]) HSP 1 Score: 168.318 bits (425), Expect = 2.837e-43 Identity = 138/454 (30.40%), Postives = 222/454 (48.90%), Query Frame = 1
Query: 832 PNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMK---FRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYF-ASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKL-DSED------GKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
P + + +TSGTTG+PKG M++H+N+V ++ + ++++ IS+LPL+H+ ++ + A + F Q D L +K + PT F VPR+ +I ++++ A S +K + ++A D Y ++++N SI +IFNKV ++LG R + + AAP+S VL + A+L I E YG +EC+ T + G VG LP KL D E+ EGE+C+ G++VF GY+ PE+T E D + WLH+GD+G + G L I R K + A GE IAP IE + + ++ V GD + C++ V +P D+ P + G SI ++ + K +I I + K E + +V+ P F++ G L PTLK KR + L+ I+ LY
Sbjct: 253 PEDLSVICFTSGTTGDPKGAMITHENIVADLAGVIKIFESSFVPSTQDVSISFLPLAHMFERIVQTAV-YGAGARVGFYQGDIR--LLPDDMKTLQPTIFPVVPRLLNRIYDKVQSGAK--SPFQKWLLNFAVDRKYAEVQEGIIRKN-----SIWDKLIFNKVQESLG-GRVRLMVTGAAPISPAVLKFLRAALGCQIFEGYGQTECAAACTFTMPGDATTGHVGMPLPCNYMKLADVEEMSYFASNGEGEVCIKGKNVFKGYLKDPERTAEALDKDGWLHTGDIGKWLPSGVLKIIDRKKNIFKLAQGEYIAPEKIEN-VYVRSEAVAQVFVHGDSLQ--------SCLVAIV--VPDADVLPGFAKKLGHQG----SIEELCKNSEIKKAI-ITDMTKLGREAGLKSFEQVKDVYLHPEMFTIENGLLTPTLKAKRAELKALFLPQIDQLY 679
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Match: SMESG000080395.1 (SMESG000080395.1) HSP 1 Score: 968.763 bits (2503), Expect = 0.000e+0 Identity = 466/468 (99.57%), Postives = 467/468 (99.79%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE+GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSI IDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE
Sbjct: 114 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEDGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSICIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 581 HSP 2 Score: 214.542 bits (545), Expect = 3.296e-60 Identity = 104/110 (94.55%), Postives = 105/110 (95.45%), Query Frame = 2
Query: 404 LIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDVYVFL 733
L IGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYD F+
Sbjct: 9 LYNIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDWTEFM 118
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Match: SMESG000080395.1 (SMESG000080395.1) HSP 1 Score: 968.378 bits (2502), Expect = 0.000e+0 Identity = 466/468 (99.57%), Postives = 467/468 (99.79%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE+GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSI IDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE
Sbjct: 109 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEDGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSICIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 576 HSP 2 Score: 214.927 bits (546), Expect = 2.521e-60 Identity = 103/108 (95.37%), Postives = 105/108 (97.22%), Query Frame = 2
Query: 410 QIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDVYVFL 733
+IGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYD F+
Sbjct: 6 EIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDWTEFM 113
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Match: SMESG000080395.1 (SMESG000080395.1) HSP 1 Score: 959.518 bits (2479), Expect = 0.000e+0 Identity = 462/464 (99.57%), Postives = 463/464 (99.78%), Query Frame = 1
Query: 772 MNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 2163
MNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKE+GPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSI IDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE
Sbjct: 1 MNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKEDGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKEGEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSICIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLYE 464
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Match: SMESG000027204.1 (SMESG000027204.1) HSP 1 Score: 506.523 bits (1303), Expect = 3.967e-171 Identity = 247/468 (52.78%), Postives = 334/468 (71.37%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
WTEFMNL + D L RL+ + P++C LIYTSGTTG PKGVM+SHDN+++T + + ++K + K +ISYLPLSH+AA + DI+ + T++FAQPDALKGSLG TLKEV PT FIGVPRVFEKI EQM+E+ ++KK I+ WAK++G+R +LAK+ + Y +AK ++F+K+ +ALG DRC+ + SAAAPL+ L YF SLD+ I +IYG+SEC+GP T+ ++ R GS G IL K+D + GEIC+ GRHVF+GY N EKTKE DS+ WLHSGD+G D +L+ITGRIKEL+ITAGGENIAPVPIE KIKE + FIS+ M+VGD++K++ LLT+K +D TQ PT+ LTPAA R IG ++ +++ K+ L IDE IK+ N ++SR+A VQKW+ LP DFS+ GGELGPT+K+KR + E+YS +++S+Y
Sbjct: 120 WTEFMNLNQADSADELDRRLSEIQPDDCALLIYTSGTTGFPKGVMLSHDNMIFTVKKAMEHVKIQPFKNSVISYLPLSHIAANLNDIMTTVSVGVTVYFAQPDALKGSLGKTLKEVRPTVFIGVPRVFEKIQEQMQEIGSKAGTIKKYIAGWAKNVGHRNSLAKITGKPVEKSYFLAKYLVFDKIKEALGLDRCIYLASAAAPLTQQSLDYFLSLDMPINQIYGLSECTGPHTMGITDKIRIGSAGTILKGVEMKIDEPNEHNIGEICIRGRHVFLGYYNNMEKTKESIDSDGWLHSGDLGKVEDNEHLYITGRIKELIITAGGENIAPVPIEDKIKERIPFISSVMLVGDRQKFVSCLLTLKSTVDVDTQRPTNQLTPAAIRSLKDIGIEAKTVEEVLECKDIALKKFIDEAIKKVNSCSVSRAAYVQKWSLLPRDFSISGGELGPTMKMKRMAIVEMYSEIVSSMY 587 HSP 2 Score: 101.679 bits (252), Expect = 4.061e-22 Identity = 48/122 (39.34%), Postives = 73/122 (59.84%), Query Frame = 2
Query: 368 QYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDVYVFL 733
QY +L AA + I++GL+ + +V I G N P WFI+ G IFAGG+ GIY TN + +FI+N+C C + +V N++ L K + + + P LKAI+ Y G + +K IS+Y F+
Sbjct: 3 QYLELIQKAASNFIKLGLDVKAAVGILGFNHPAWFISQLGGIFAGGIICGIYTTNTPDICKFIMNDCNCQIIVVENDDQLNKILSILPDVPQLKAIIKYTGDVSAELKTKYSISIYTWTEFM 124
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Match: SMESG000027204.1 (SMESG000027204.1) HSP 1 Score: 506.138 bits (1302), Expect = 9.212e-170 Identity = 247/468 (52.78%), Postives = 334/468 (71.37%), Query Frame = 1
Query: 760 WTEFMNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTAECMLYYMKFRHSKEMLISYLPLSHVAAQMCDIIIPLLCQATIHFAQPDALKGSLGHTLKEVHPTYFIGVPRVFEKIMEQMKEMAIDFSSVKKSISSWAKDIGYRTNLAKMMKENGPFGYSIAKCVIFNKVHKALGFDRCLGIFSAAAPLSHDVLVYFASLDLVICEIYGMSECSGPQTIEFQNEFRFGSVGKILPDFGCKLDSEDGKE-GEICMYGRHVFMGYVNAPEKTKEVFDSEYWLHSGDVGTFNDEGYLFITGRIKELLITAGGENIAPVPIEQKIKENLDFISNAMVVGDKRKYLCVLLTIKCVIDPVTQIPTDDLTPAAKRWFSGIGYNPTSISQIITDKNPKLSISIDEGIKRSNENAISRSAEVQKWTFLPHDFSVPGGELGPTLKLKRNVVNELYSALINSLY 2160
WTEFMNL + D L RL+ + P++C LIYTSGTTG PKGVM+SHDN+++T + + ++K + K +ISYLPLSH+AA + DI+ + T++FAQPDALKGSLG TLKEV PT FIGVPRVFEKI EQM+E+ ++KK I+ WAK++G+R +LAK+ + Y +AK ++F+K+ +ALG DRC+ + SAAAPL+ L YF SLD+ I +IYG+SEC+GP T+ ++ R GS G IL K+D + GEIC+ GRHVF+GY N EKTKE DS+ WLHSGD+G D +L+ITGRIKEL+ITAGGENIAPVPIE KIKE + FIS+ M+VGD++K++ LLT+K +D TQ PT+ LTPAA R IG ++ +++ K+ L IDE IK+ N ++SR+A VQKW+ LP DFS+ GGELGPT+K+KR + E+YS +++S+Y
Sbjct: 211 WTEFMNLNQADSADELDRRLSEIQPDDCALLIYTSGTTGFPKGVMLSHDNMIFTVKKAMEHVKIQPFKNSVISYLPLSHIAANLNDIMTTVSVGVTVYFAQPDALKGSLGKTLKEVRPTVFIGVPRVFEKIQEQMQEIGSKAGTIKKYIAGWAKNVGHRNSLAKITGKPVEKSYFLAKYLVFDKIKEALGLDRCIYLASAAAPLTQQSLDYFLSLDMPINQIYGLSECTGPHTMGITDKIRIGSAGTILKGVEMKIDEPNEHNIGEICIRGRHVFLGYYNNMEKTKESIDSDGWLHSGDLGKVEDNEHLYITGRIKELIITAGGENIAPVPIEDKIKERIPFISSVMLVGDRQKFVSCLLTLKSTVDVDTQRPTNQLTPAAIRSLKDIGIEAKTVEEVLECKDIALKKFIDEAIKKVNSCSVSRAAYVQKWSLLPRDFSISGGELGPTMKMKRMAIVEMYSEIVSSMY 678 HSP 2 Score: 106.686 bits (265), Expect = 1.553e-23 Identity = 52/140 (37.14%), Postives = 82/140 (58.57%), Query Frame = 2
Query: 317 LKYKYSQESSF-LSINYSQYYQLCCMAAKSLIQIGLNARKSVCISGHNSPEWFITYFGTIFAGGVASGIYVTNNKETVEFIINNCECIVAIVANEELLKKYVCVSKNCPNLKAIVYYGGLIDDSIKGGCKISLYDVYVFL 733
LKYK ++ + + + QY +L AA + I++GL+ + +V I G N P WFI+ G IFAGG+ GIY TN + +FI+N+C C + +V N++ L K + + + P LKAI+ Y G + +K IS+Y F+
Sbjct: 76 LKYKSDPKNGYDIEVTMEQYLELIQKAASNFIKLGLDVKAAVGILGFNHPAWFISQLGGIFAGGIICGIYTTNTPDICKFIMNDCNCQIIVVENDDQLNKILSILPDVPQLKAIIKYTGDVSAELKTKYSISIYTWTEFM 215 The following BLAST results are available for this feature:
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Long-chain-fatty-acid--CoA ligase ACSBG2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033222 ID=SMED30033222|Name=Long-chain-fatty-acid--CoA ligase ACSBG2|organism=Schmidtea mediterranea sexual|type=transcript|length=2393bpback to top protein sequence of SMED30033222-orf-1 >SMED30033222-orf-1 ID=SMED30033222-orf-1|Name=SMED30033222-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=465bp MNLGININDDTLIGRLNHLIPNECCSLIYTSGTTGNPKGVMMSHDNLVWTback to top protein sequence of SMED30033222-orf-2 >SMED30033222-orf-2 ID=SMED30033222-orf-2|Name=SMED30033222-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp MEVDHVKLIKVNGPLNKSQNKITFEIKQHSPVETENMFSTSLDKVSKLRMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
