SMED30033197

Overview


Name	SMED30033197
Smed ID	SMED30033197
Length (bp)	355

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30033197 (SMED30033197) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033197 (SMED30033197) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033197 (SMED30033197) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033197 (SMED30033197) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30033197

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
pharynx	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30033197	Contig52091	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30033197	Contig52091	newmark_ests	PMID:29674431 Fincher et al., 2018
head region	SMED30033197	dd_Smed_v6_116_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30033197	OX_Smed_1.0.19974	ox_Smed_v2	PMID:24238224 Kao et al., 2013
epidermis	SMED30033197	dd_Smed_v6_116_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
Category 3 cell	SMED30033197	dd_Smed_v6_116_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
epidermis	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
Category 4 cell	SMED30033197	dd_Smed_v4_116_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30033197 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000933:67860..68245 +	1465

Homology

[Close]

BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL2 (dynein light chain LC8-type 2 [Source:HGNC Symbol;Acc:HGNC:24596])

HSP 1 Score: 135.961 bits (341), Expect = 1.678e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 130.183 bits (326), Expect = 3.566e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 132.88 bits (333), Expect = 1.107e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-5 (Dynein Light Chain [Source:UniProtKB/TrEMBL;Acc:Q65XS9])

HSP 1 Score: 69.3218 bits (168), Expect = 9.959e-16
Identity = 30/80 (37.50%), Postives = 46/80 (57.50%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            ++ M   M++ A + A  ++  Y +E DIA  +K  FD+   P W C  G++FGS+V  E   FIYF  G +A +LFK+ 
Sbjct:   62 HSRMPRHMEQEACSLAAKSIMTYHLEHDIARHLKMAFDREYGPDWHCICGKHFGSFVTFEPDSFIYFRIGTIAFMLFKTS 141

[Close]

BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-2 (Probable dynein light chain 2, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q21557])

HSP 1 Score: 60.4622 bits (145), Expect = 3.894e-13
Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = -3

Query:  114 ADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 350
             DM++  ++  ++    A   Y I+KD+AAF+K+E DK    TW    G+ FGS V +E   FI     +V ++++K G
Sbjct:   11 TDMEDPQRDMVISVVREAQRLYNIDKDVAAFVKEELDKKFGATWHVICGKCFGSRVSYEMGHFILLKCNKVNVMIYKCG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077924)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0305553)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077925)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070706)

HSP 1 Score: 132.109 bits (331), Expect = 2.652e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070704)

HSP 1 Score: 132.109 bits (331), Expect = 2.652e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll2a (dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:ZDB-GENE-030828-11])

HSP 1 Score: 135.961 bits (341), Expect = 1.115e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll2b (dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:ZDB-GENE-050320-132])

HSP 1 Score: 135.961 bits (341), Expect = 1.115e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 132.494 bits (332), Expect = 2.987e-41
Identity = 63/80 (78.75%), Postives = 67/80 (83.75%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Xenopus
Match: dynll2 (dynein light chain LC8-type 2 [Source:NCBI gene;Acc:100038056])

HSP 1 Score: 135.961 bits (341), Expect = 1.521e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Xenopus
Match: dynll1 (dynein light chain LC8-type 1 [Source:NCBI gene;Acc:448652])

HSP 1 Score: 132.494 bits (332), Expect = 3.857e-41
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EK+ IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Xenopus
Match: ENSXETT00000015639.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:48978690:48979872:1 gene:ENSXETG00000009974.1 transcript:ENSXETT00000015639.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 5.979e-13
Identity = 35/81 (43.21%), Postives = 47/81 (58.02%), Query Frame = 3

Query:  111 LSGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            L+ FK +N NLA IK++E  CFM  IA+KVS +   P        +  D  SN+FL  + LH   G V+S  LH+F H+SI
Sbjct:    3 LARFKEENCNLAQIKVDEVLCFMCYIAAKVSANDAVP-------IYLLDIGSNVFLNIILLHCLCGTVNSILLHVFRHVSI 76

[Close]

BLAST of SMED30033197 vs. Ensembl Xenopus
Match: rnf181 (ring finger protein 181 [Source:Xenbase;Acc:XB-GENE-964051])

HSP 1 Score: 48.9062 bits (115), Expect = 3.321e-8
Identity = 27/80 (33.75%), Postives = 42/80 (52.50%), Query Frame = -3

Query:  120 NADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 353
            + DM E+M+   +    TA EK+    + AA  IK+  DK    +W   +G  FG  + HE K  +Y F+ G +AI ++K
Sbjct:   25 HTDMPEEMRVETMELCVTACEKFASNNESAAKMIKETMDKKFGSSWHVVIGEGFGFEITHEVKNLLYMFFGGSLAICVWK 104

[Close]

BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll2 (dynein light chain LC8-type 2 [Source:MGI Symbol;Acc:MGI:1915347])

HSP 1 Score: 135.961 bits (341), Expect = 1.152e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll1 (dynein light chain LC8-type 1 [Source:MGI Symbol;Acc:MGI:1861457])

HSP 1 Score: 130.183 bits (326), Expect = 2.448e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q78P75|DYL2_RAT (Dynein light chain 2, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q9D0M5|DYL2_MOUSE (Dynein light chain 2, cytoplasmic OS=Mus musculus OX=10090 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q96FJ2|DYL2_HUMAN (Dynein light chain 2, cytoplasmic OS=Homo sapiens OX=9606 GN=DYNLL2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q3MHR3|DYL2_BOVIN (Dynein light chain 2, cytoplasmic OS=Bos taurus OX=9913 GN=DYNLL2 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|O96860|DYL2_DROME (Dynein light chain 2, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=Cdlc2 PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.488e-41
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. TrEMBL
Match: V9L8Z1 (Dynein light chain (Fragment) OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 8.372e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   46 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 125

[Close]

BLAST of SMED30033197 vs. TrEMBL
Match: A0A4W3IBH7 (Dynein light chain OS=Callorhinchus milii OX=7868 GN=dynll2 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.004e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. TrEMBL
Match: A0A401QGE4 (Dynein light chain OS=Scyliorhinus torazame OX=75743 GN=scyTo_0025139 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.004e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. TrEMBL
Match: A0A3B5K1U3 (Dynein light chain OS=Takifugu rubripes OX=31033 GN=LOC101078390 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.304e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. TrEMBL
Match: A0A3Q3MX83 (Dynein light chain OS=Mastacembelus armatus OX=205130 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.304e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Cavefish
Match: ENSAMXT00000047143.1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 135.961 bits (341), Expect = 9.451e-43
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dynll2b (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 135.961 bits (341), Expect = 9.451e-43
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 113.62 bits (283), Expect = 1.336e-33
Identity = 54/71 (76.06%), Postives = 58/71 (81.69%), Query Frame = -3

Query:  141 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQ 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQ
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQ 80

[Close]

BLAST of SMED30033197 vs. Ensembl Cavefish
Match: ENSAMXT00000013314.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:23335261:23337700:1 gene:ENSAMXG00000012944.2 transcript:ENSAMXT00000013314.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 4.726e-9
Identity = 23/83 (27.71%), Postives = 43/83 (51.81%), Query Frame = 3

Query:  105 IKLSGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            I  + F+ +  +LA ++++E    + D+ ++V  H   P W+   + + FD   ++ LY V LH     VD   LH+  H+S+
Sbjct:    1 IASARFEQEYGHLAQVEVDEVLRLVGDVTAEVPAHNAVPSWVV-LLAYLFDVRCDVLLYVVLLHCLCCTVDCVLLHVLRHVSV 82

[Close]

BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dnal4b (dynein axonemal light chain 4 [Source:NCBI gene;Acc:103029781])

HSP 1 Score: 50.0618 bits (118), Expect = 7.816e-9
Identity = 27/80 (33.75%), Postives = 42/80 (52.50%), Query Frame = -3

Query:  120 NADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 353
            + DM E+M+   +    TA EK+    + AA  IK+  DK    +W   +G  FG  V HE K  +Y F+ G +A+ ++K
Sbjct:   25 HTDMPEEMRVETMELCVTACEKFATNNESAAKMIKESMDKKFGSSWHVVIGEGFGFEVTHEVKNLLYMFFGGSLAVCVWK 104

[Close]

BLAST of SMED30033197 vs. Ensembl Yeast
Match: DYN2 (Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments [Source:SGD;Acc:S000002832])

HSP 1 Score: 77.411 bits (189), Expect = 1.525e-20
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = -3

Query:  117 ADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKS 350
            +D+ + ++E  +  +  A++KY +E+DIA  +KK+ D     TW   VG+NFGSYV HE   F+YFY G +A L+FK+
Sbjct:   14 SDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKNFGSYVTHEKGHFVYFYIGPLAFLVFKT 91

[Close]

BLAST of SMED30033197 vs. Ensembl Nematostella
Match: EDO44582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI5])

HSP 1 Score: 131.724 bits (330), Expect = 1.634e-41
Identity = 63/80 (78.75%), Postives = 66/80 (82.50%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ  A+  AT A+EK+ IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMAEDMQTDAIECATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Nematostella
Match: EDO38557 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCP2])

HSP 1 Score: 50.447 bits (119), Expect = 2.697e-9
Identity = 27/79 (34.18%), Postives = 42/79 (53.16%), Query Frame = -3

Query:  120 ADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 350
            +DM ++M+  A+    TA EK+    + AA  IK+  DK    +W   VG  FG  + HE +  +Y F+ G  AI ++K
Sbjct:   30 SDMNDEMRTEAMELCVTACEKFSNNNETAAKMIKESMDKKFGASWHAVVGEGFGFEITHEVRNLLYMFFGGTTAICIWK 108

[Close]

BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000005747.2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101162462])

HSP 1 Score: 134.035 bits (336), Expect = 4.809e-42
Identity = 64/80 (80.00%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Medaka
Match: dynll1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101171417])

HSP 1 Score: 134.035 bits (336), Expect = 5.425e-42
Identity = 64/80 (80.00%), Postives = 67/80 (83.75%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Medaka
Match: DYNLL2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101174042])

HSP 1 Score: 130.183 bits (326), Expect = 1.932e-40
Identity = 61/80 (76.25%), Postives = 67/80 (83.75%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM ++MQ+ AV+ A  AMEKY IEKDIAA++KKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDEMQQDAVDCAMQAMEKYNIEKDIAAYVKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000006686.2 (pep primary_assembly:ASM223467v1:14:12601680:12602092:1 gene:ENSORLG00000005302.2 transcript:ENSORLT00000006686.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 1.021e-9
Identity = 29/85 (34.12%), Postives = 43/85 (50.59%), Query Frame = 3

Query:  114 SGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFF-----FDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            +G K +NS+L  ++++E    +  IA+KV P+   P  +   ++F       D CSNIFL  V LH   G V    LH   H+ I
Sbjct:   19 AGLKQQNSHLTQVEVDEMLRLVGHIATKVPPNNAVPCRVVFLVKFLDPTYLLDICSNIFLDIVLLHGLHGTVHCILLHFIRHVCI 103

[Close]

BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000029240.1 (pep primary_assembly:ASM223467v1:13:10253300:10255192:1 gene:ENSORLG00000024686.1 transcript:ENSORLT00000029240.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.311e-9
Identity = 26/78 (33.33%), Postives = 42/78 (53.85%), Query Frame = 3

Query:  120 FK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
             K +N  LA +++N+  C + D+A++VS +   PG +   +       SN+ LY VFLH   G V    LH+  H+S+
Sbjct:    5 LKEQNGYLAQVEVNKMLCLVGDVAAEVSANDAVPGRVV-LLVELLKLGSNVLLYVVFLHGLCGTVHGILLHLLGHVSV 81

[Close]

BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000073811.1 (SMESG000073811.1)

HSP 1 Score: 149.828 bits (377), Expect = 2.271e-48
Identity = 72/80 (90.00%), Postives = 72/80 (90.00%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADMQEDMQEAAVN ATTAMEKY IEKDIAAFIKKEFDKL NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMQEDMQEAAVNCATTAMEKYNIEKDIAAFIKKEFDKLYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000005286.1 (SMESG000005286.1)

HSP 1 Score: 135.961 bits (341), Expect = 8.179e-43
Identity = 64/80 (80.00%), Postives = 69/80 (86.25%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM ++MQEAA+N AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMTDEMQEAAINYATQAMEKFNIEKDIAAYIKKEFDKSFNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000043518.1 (SMESG000043518.1)

HSP 1 Score: 133.265 bits (334), Expect = 9.568e-42
Identity = 64/80 (80.00%), Postives = 67/80 (83.75%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV  AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   12 NADMSEDMQQDAVECATQAMEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 91

[Close]

BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000003543.1 (SMESG000003543.1)

HSP 1 Score: 132.109 bits (331), Expect = 2.958e-41
Identity = 62/80 (77.50%), Postives = 67/80 (83.75%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A+  AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   12 NADMADDMQQEAIECATQAMEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 91

[Close]

BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000017229.1 (SMESG000017229.1)

HSP 1 Score: 128.642 bits (322), Expect = 5.865e-40
Identity = 59/80 (73.75%), Postives = 66/80 (82.50%), Query Frame = -3

Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ A  AMEKY +EKDIAA+IKKEFDK  NPTW   VGRNFGSYV HETK FIYFY GQVA+LLFKSG
Sbjct:   10 NADMSDDMQQEAIDVALQAMEKYNVEKDIAAYIKKEFDKKYNPTWHAIVGRNFGSYVTHETKHFIYFYLGQVAVLLFKSG 89

The following BLAST results are available for this feature:

BLAST of SMED30033197 vs. Ensembl Human
BLAST of SMED30033197 vs. Ensembl Celegans
BLAST of SMED30033197 vs. Ensembl Fly
BLAST of SMED30033197 vs. Ensembl Zebrafish
BLAST of SMED30033197 vs. Ensembl Xenopus
BLAST of SMED30033197 vs. Ensembl Mouse
BLAST of SMED30033197 vs. UniProt/SwissProt
BLAST of SMED30033197 vs. TrEMBL
BLAST of SMED30033197 vs. Ensembl Cavefish
BLAST of SMED30033197 vs. Ensembl Sea Lamprey
BLAST of SMED30033197 vs. Ensembl Yeast
BLAST of SMED30033197 vs. Ensembl Nematostella
BLAST of SMED30033197 vs. Ensembl Medaka
BLAST of SMED30033197 vs. Planmine SMEST

BLAST of SMED30033197 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DYNLL2	1.678e-42	81.25	dynein light chain LC8-type 2 [Source:HGNC Symbol;...	[more]
DYNLL1	3.566e-40	77.50	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.566e-40	77.50	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.566e-40	77.50	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.566e-40	77.50	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]

BLAST of SMED30033197 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dlc-1	1.107e-41	77.50	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]
dlc-1	1.107e-41	77.50	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]
dlc-1	1.107e-41	77.50	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]
dlc-5	9.959e-16	37.50	Dynein Light Chain [Source:UniProtKB/TrEMBL;Acc:Q...	[more]
dlc-2	3.894e-13	34.18	Probable dynein light chain 2, cytoplasmic [Sourc...	[more]

BLAST of SMED30033197 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Cdlc2	9.465e-42	78.75	gene:FBgn0026141 transcript:FBtr0077924	[more]
Cdlc2	9.465e-42	78.75	gene:FBgn0026141 transcript:FBtr0305553	[more]
Cdlc2	9.465e-42	78.75	gene:FBgn0026141 transcript:FBtr0077925	[more]
ctp	2.652e-41	77.50	gene:FBgn0011760 transcript:FBtr0070706	[more]
ctp	2.652e-41	77.50	gene:FBgn0011760 transcript:FBtr0070704	[more]

BLAST of SMED30033197 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dynll2a	1.115e-42	81.25	dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:...	[more]
dynll2b	1.115e-42	81.25	dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:...	[more]
dynll2a	1.115e-42	81.25	dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:...	[more]
dynll1	2.987e-41	78.75	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]
dynll1	2.987e-41	78.75	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]

BLAST of SMED30033197 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
dynll2	1.521e-42	81.25	dynein light chain LC8-type 2 [Source:NCBI gene;Ac...	[more]
dynll1	3.857e-41	78.75	dynein light chain LC8-type 1 [Source:NCBI gene;Ac...	[more]
ENSXETT00000015639.1	5.979e-13	43.21	pep primary_assembly:Xenopus_tropicalis_v9.1:2:489...	[more]
rnf181	3.321e-8	33.75	ring finger protein 181 [Source:Xenbase;Acc:XB-GEN...	[more]

BLAST of SMED30033197 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Dynll2	1.152e-42	81.25	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll2	1.152e-42	81.25	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll2	1.152e-42	81.25	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll1	2.448e-40	77.50	dynein light chain LC8-type 1 [Source:MGI Symbol;A...	[more]
Dynll1	2.448e-40	77.50	dynein light chain LC8-type 1 [Source:MGI Symbol;A...	[more]

BLAST of SMED30033197 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q78P75\|DYL2_RAT	8.058e-42	81.25	Dynein light chain 2, cytoplasmic OS=Rattus norveg...	[more]
sp\|Q9D0M5\|DYL2_MOUSE	8.058e-42	81.25	Dynein light chain 2, cytoplasmic OS=Mus musculus ...	[more]
sp\|Q96FJ2\|DYL2_HUMAN	8.058e-42	81.25	Dynein light chain 2, cytoplasmic OS=Homo sapiens ...	[more]
sp\|Q3MHR3\|DYL2_BOVIN	8.058e-42	81.25	Dynein light chain 2, cytoplasmic OS=Bos taurus OX...	[more]
sp\|O96860\|DYL2_DROME	9.488e-41	78.75	Dynein light chain 2, cytoplasmic OS=Drosophila me...	[more]

BLAST of SMED30033197 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
V9L8Z1	8.372e-40	82.50	Dynein light chain (Fragment) OS=Callorhinchus mil...	[more]
A0A4W3IBH7	9.004e-40	82.50	Dynein light chain OS=Callorhinchus milii OX=7868 ...	[more]
A0A401QGE4	9.004e-40	82.50	Dynein light chain OS=Scyliorhinus torazame OX=757...	[more]
A0A3B5K1U3	9.304e-40	82.50	Dynein light chain OS=Takifugu rubripes OX=31033 G...	[more]
A0A3Q3MX83	9.304e-40	82.50	Dynein light chain OS=Mastacembelus armatus OX=205...	[more]

BLAST of SMED30033197 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000047143.1	9.451e-43	81.25	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
dynll2b	9.451e-43	81.25	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
dynll1	1.336e-33	76.06	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]
ENSAMXT00000013314.2	4.726e-9	27.71	pep primary_assembly:Astyanax_mexicanus-2.0:1:2333...	[more]
dnal4b	7.816e-9	33.75	dynein axonemal light chain 4 [Source:NCBI gene;Ac...	[more]

BLAST of SMED30033197 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033197 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
DYN2	1.525e-20	42.31	Cytoplasmic light chain dynein, microtubule motor ...	[more]

BLAST of SMED30033197 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO44582	1.634e-41	78.75	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38557	2.697e-9	34.18	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30033197 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000005747.2	4.809e-42	80.00	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
dynll1	5.425e-42	80.00	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
DYNLL2	1.932e-40	76.25	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
ENSORLT00000006686.2	1.021e-9	34.12	pep primary_assembly:ASM223467v1:14:12601680:12602...	[more]
ENSORLT00000029240.1	2.311e-9	33.33	pep primary_assembly:ASM223467v1:13:10253300:10255...	[more]

BLAST of SMED30033197 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000073811.1	2.271e-48	90.00	SMESG000073811.1	[more]
SMESG000005286.1	8.179e-43	80.00	SMESG000005286.1	[more]
SMESG000043518.1	9.568e-42	80.00	SMESG000043518.1	[more]
SMESG000003543.1	2.958e-41	77.50	SMESG000003543.1	[more]
SMESG000017229.1	5.865e-40	73.75	SMESG000017229.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30033197 ID=SMED30033197|Name=SMED30033197|organism=Schmidtea mediterranea sexual|type=transcript|length=355bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTCAATATATAT
TTATTAAAATCCTAACAAAAAACTCTGATAAAAATCAAAATTTTATTTTA
TGGAATAAAATTATCCGGATTTAAATAAAAGAATAGCAACTTGGCCCANA
TAAAAATAAATGAAANGTTTTGTTTCATGAGNGACATAGCTTCCAAAGTT
TCTCCCCACANTACAGNGCCAGGTTGGATTGGATAATTTATCGAATTCTT
TTTTGATAAATGCAGCAATATCTTTCTCTATGNTGTATTTCTCCATCGCT
GTCGTGGCNCAGTTGACAGCAGCTTCCTGCATATCTTCTTGCATATCAGC
ATTTT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000018	Category 4 cell
PLANA:0000034	epidermis
PLANA:0000035	Category 3 cell
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR037177	DLC_sf
IPR019763	Dynein_light_1/2_CS
IPR001372	Dynein_light_chain_typ-1/2

Vocabulary: **cellular component**
Term	Definition
GO:0005875	microtubule associated complex
GO:0030286	dynein complex

Vocabulary: **biological process**
Term	Definition
GO:0007017	microtubule-based process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001372	Dynein light chain, type 1/2	SMART	SM01375	Dynein_light_2	coord: 1..79 e-value: 4.1E-49 score: 179.2
IPR001372	Dynein light chain, type 1/2	PFAM	PF01221	Dynein_light	coord: 1..79 e-value: 1.3E-39 score: 134.3
IPR001372	Dynein light chain, type 1/2	PANTHER	PTHR11886	DYNEIN LIGHT CHAIN	coord: 1..80
IPR037177	Dynein light chain superfamily	GENE3D	G3DSA:3.30.740.10		coord: 1..80 e-value: 1.3E-43 score: 149.0
IPR037177	Dynein light chain superfamily	SUPERFAMILY	SSF54648	DLC	coord: 1..80
None	No IPR available	PANTHER	PTHR11886:SF62	DYNEIN LIGHT CHAIN	coord: 1..80
IPR019763	Dynein light chain, type 1/2, conserved site	PROSITE	PS01239	DYNEIN_LIGHT_1	coord: 46..60

Main menu

SMED30033197

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

blazegraph-runner