SMED30033197

Overview
NameSMED30033197
Smed IDSMED30033197
Length (bp)355
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033197 (SMED30033197) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033197 (SMED30033197) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033197 (SMED30033197) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033197 (SMED30033197) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033197

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033197SMESG000073811.1 Contig52091newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033197SMESG000073811.1 Contig52091uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30033197SMESG000073811.1 dd_Smed_v6_116_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30033197SMESG000073811.1 OX_Smed_1.0.19974ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30033197SMESG000073811.1 dd_Smed_v6_116_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30033197SMESG000073811.1 dd_Smed_v6_116_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30033197SMESG000073811.1 dd_Smed_v4_116_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033197 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000933:67860..68245 +1465
Homology
BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL2 (dynein light chain LC8-type 2 [Source:HGNC Symbol;Acc:HGNC:24596])

HSP 1 Score: 135.961 bits (341), Expect = 1.678e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 130.183 bits (326), Expect = 3.566e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 130.183 bits (326), Expect = 3.566e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 130.183 bits (326), Expect = 3.566e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 130.183 bits (326), Expect = 3.566e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 132.88 bits (333), Expect = 1.107e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 132.88 bits (333), Expect = 1.107e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 132.88 bits (333), Expect = 1.107e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-5 (Dynein Light Chain [Source:UniProtKB/TrEMBL;Acc:Q65XS9])

HSP 1 Score: 69.3218 bits (168), Expect = 9.959e-16
Identity = 30/80 (37.50%), Postives = 46/80 (57.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            ++ M   M++ A + A  ++  Y +E DIA  +K  FD+   P W C  G++FGS+V  E   FIYF  G +A +LFK+ 
Sbjct:   62 HSRMPRHMEQEACSLAAKSIMTYHLEHDIARHLKMAFDREYGPDWHCICGKHFGSFVTFEPDSFIYFRIGTIAFMLFKTS 141          
BLAST of SMED30033197 vs. Ensembl Celegans
Match: dlc-2 (Probable dynein light chain 2, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q21557])

HSP 1 Score: 60.4622 bits (145), Expect = 3.894e-13
Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = -3
Query:  114 ADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 350
             DM++  ++  ++    A   Y I+KD+AAF+K+E DK    TW    G+ FGS V +E   FI     +V ++++K G
Sbjct:   11 TDMEDPQRDMVISVVREAQRLYNIDKDVAAFVKEELDKKFGATWHVICGKCFGSRVSYEMGHFILLKCNKVNVMIYKCG 89          
BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077924)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0305553)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077925)

HSP 1 Score: 133.265 bits (334), Expect = 9.465e-42
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070706)

HSP 1 Score: 132.109 bits (331), Expect = 2.652e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070704)

HSP 1 Score: 132.109 bits (331), Expect = 2.652e-41
Identity = 62/80 (77.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll2a (dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:ZDB-GENE-030828-11])

HSP 1 Score: 135.961 bits (341), Expect = 1.115e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll2b (dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:ZDB-GENE-050320-132])

HSP 1 Score: 135.961 bits (341), Expect = 1.115e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll2a (dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:ZDB-GENE-030828-11])

HSP 1 Score: 135.961 bits (341), Expect = 1.115e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 132.494 bits (332), Expect = 2.987e-41
Identity = 63/80 (78.75%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Zebrafish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 132.494 bits (332), Expect = 2.987e-41
Identity = 63/80 (78.75%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Xenopus
Match: dynll2 (dynein light chain LC8-type 2 [Source:NCBI gene;Acc:100038056])

HSP 1 Score: 135.961 bits (341), Expect = 1.521e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Xenopus
Match: dynll1 (dynein light chain LC8-type 1 [Source:NCBI gene;Acc:448652])

HSP 1 Score: 132.494 bits (332), Expect = 3.857e-41
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EK+ IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Xenopus
Match: ENSXETT00000015639.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:48978690:48979872:1 gene:ENSXETG00000009974.1 transcript:ENSXETT00000015639.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 5.979e-13
Identity = 35/81 (43.21%), Postives = 47/81 (58.02%), Query Frame = 3
Query:  111 LSGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            L+ FK +N NLA IK++E  CFM  IA+KVS +   P        +  D  SN+FL  + LH   G V+S  LH+F H+SI
Sbjct:    3 LARFKEENCNLAQIKVDEVLCFMCYIAAKVSANDAVP-------IYLLDIGSNVFLNIILLHCLCGTVNSILLHVFRHVSI 76          
BLAST of SMED30033197 vs. Ensembl Xenopus
Match: rnf181 (ring finger protein 181 [Source:Xenbase;Acc:XB-GENE-964051])

HSP 1 Score: 48.9062 bits (115), Expect = 3.321e-8
Identity = 27/80 (33.75%), Postives = 42/80 (52.50%), Query Frame = -3
Query:  120 NADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 353
            + DM E+M+   +    TA EK+    + AA  IK+  DK    +W   +G  FG  + HE K  +Y F+ G +AI ++K
Sbjct:   25 HTDMPEEMRVETMELCVTACEKFASNNESAAKMIKETMDKKFGSSWHVVIGEGFGFEITHEVKNLLYMFFGGSLAICVWK 104          
BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll2 (dynein light chain LC8-type 2 [Source:MGI Symbol;Acc:MGI:1915347])

HSP 1 Score: 135.961 bits (341), Expect = 1.152e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll2 (dynein light chain LC8-type 2 [Source:MGI Symbol;Acc:MGI:1915347])

HSP 1 Score: 135.961 bits (341), Expect = 1.152e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll2 (dynein light chain LC8-type 2 [Source:MGI Symbol;Acc:MGI:1915347])

HSP 1 Score: 135.961 bits (341), Expect = 1.152e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll1 (dynein light chain LC8-type 1 [Source:MGI Symbol;Acc:MGI:1861457])

HSP 1 Score: 130.183 bits (326), Expect = 2.448e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Mouse
Match: Dynll1 (dynein light chain LC8-type 1 [Source:MGI Symbol;Acc:MGI:1861457])

HSP 1 Score: 130.183 bits (326), Expect = 2.448e-40
Identity = 62/80 (77.50%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ +V  AT A+EKY IEKDIAA IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q78P75|DYL2_RAT (Dynein light chain 2, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q9D0M5|DYL2_MOUSE (Dynein light chain 2, cytoplasmic OS=Mus musculus OX=10090 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q96FJ2|DYL2_HUMAN (Dynein light chain 2, cytoplasmic OS=Homo sapiens OX=9606 GN=DYNLL2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|Q3MHR3|DYL2_BOVIN (Dynein light chain 2, cytoplasmic OS=Bos taurus OX=9913 GN=DYNLL2 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 8.058e-42
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. UniProt/SwissProt
Match: sp|O96860|DYL2_DROME (Dynein light chain 2, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=Cdlc2 PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.488e-41
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT A+EKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HET+ FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. TrEMBL
Match: V9L8Z1 (Dynein light chain (Fragment) OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 8.372e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   46 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 125          
BLAST of SMED30033197 vs. TrEMBL
Match: A0A4W3IBH7 (Dynein light chain OS=Callorhinchus milii OX=7868 GN=dynll2 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.004e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. TrEMBL
Match: A0A401QGE4 (Dynein light chain OS=Scyliorhinus torazame OX=75743 GN=scyTo_0025139 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.004e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. TrEMBL
Match: A0A3B5K1U3 (Dynein light chain OS=Takifugu rubripes OX=31033 GN=LOC101078390 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.304e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. TrEMBL
Match: A0A3Q3MX83 (Dynein light chain OS=Mastacembelus armatus OX=205130 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.304e-40
Identity = 66/80 (82.50%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Cavefish
Match: ENSAMXT00000047143.1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 135.961 bits (341), Expect = 9.451e-43
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dynll2b (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 135.961 bits (341), Expect = 9.451e-43
Identity = 65/80 (81.25%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 113.62 bits (283), Expect = 1.336e-33
Identity = 54/71 (76.06%), Postives = 58/71 (81.69%), Query Frame = -3
Query:  141 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQ 353
            NADM E+MQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQ
Sbjct:   10 NADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQ 80          
BLAST of SMED30033197 vs. Ensembl Cavefish
Match: ENSAMXT00000013314.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:23335261:23337700:1 gene:ENSAMXG00000012944.2 transcript:ENSAMXT00000013314.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 4.726e-9
Identity = 23/83 (27.71%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  105 IKLSGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            I  + F+ +  +LA ++++E    + D+ ++V  H   P W+   + + FD   ++ LY V LH     VD   LH+  H+S+
Sbjct:    1 IASARFEQEYGHLAQVEVDEVLRLVGDVTAEVPAHNAVPSWVV-LLAYLFDVRCDVLLYVVLLHCLCCTVDCVLLHVLRHVSV 82          
BLAST of SMED30033197 vs. Ensembl Cavefish
Match: dnal4b (dynein axonemal light chain 4 [Source:NCBI gene;Acc:103029781])

HSP 1 Score: 50.0618 bits (118), Expect = 7.816e-9
Identity = 27/80 (33.75%), Postives = 42/80 (52.50%), Query Frame = -3
Query:  120 NADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 353
            + DM E+M+   +    TA EK+    + AA  IK+  DK    +W   +G  FG  V HE K  +Y F+ G +A+ ++K
Sbjct:   25 HTDMPEEMRVETMELCVTACEKFATNNESAAKMIKESMDKKFGSSWHVVIGEGFGFEVTHEVKNLLYMFFGGSLAVCVWK 104          
BLAST of SMED30033197 vs. Ensembl Yeast
Match: DYN2 (Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments [Source:SGD;Acc:S000002832])

HSP 1 Score: 77.411 bits (189), Expect = 1.525e-20
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = -3
Query:  117 ADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKS 350
            +D+ + ++E  +  +  A++KY +E+DIA  +KK+ D     TW   VG+NFGSYV HE   F+YFY G +A L+FK+
Sbjct:   14 SDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKNFGSYVTHEKGHFVYFYIGPLAFLVFKT 91          
BLAST of SMED30033197 vs. Ensembl Nematostella
Match: EDO44582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI5])

HSP 1 Score: 131.724 bits (330), Expect = 1.634e-41
Identity = 63/80 (78.75%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ  A+  AT A+EK+ IEKDIAAFIKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMAEDMQTDAIECATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Nematostella
Match: EDO38557 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCP2])

HSP 1 Score: 50.447 bits (119), Expect = 2.697e-9
Identity = 27/79 (34.18%), Postives = 42/79 (53.16%), Query Frame = -3
Query:  120 ADMQEDMQEAAVNXATTAMEKYXIEKDIAA-FIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIY-FYXGQVAILLFK 350
            +DM ++M+  A+    TA EK+    + AA  IK+  DK    +W   VG  FG  + HE +  +Y F+ G  AI ++K
Sbjct:   30 SDMNDEMRTEAMELCVTACEKFSNNNETAAKMIKESMDKKFGASWHAVVGEGFGFEITHEVRNLLYMFFGGTTAICIWK 108          
BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000005747.2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101162462])

HSP 1 Score: 134.035 bits (336), Expect = 4.809e-42
Identity = 64/80 (80.00%), Postives = 68/80 (85.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM E+MQ+ AV+ AT AMEKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEEMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Medaka
Match: dynll1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101171417])

HSP 1 Score: 134.035 bits (336), Expect = 5.425e-42
Identity = 64/80 (80.00%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV  AT A+EKY IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSEDMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Medaka
Match: DYNLL2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101174042])

HSP 1 Score: 130.183 bits (326), Expect = 1.932e-40
Identity = 61/80 (76.25%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM ++MQ+ AV+ A  AMEKY IEKDIAA++KKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMSDEMQQDAVDCAMQAMEKYNIEKDIAAYVKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000006686.2 (pep primary_assembly:ASM223467v1:14:12601680:12602092:1 gene:ENSORLG00000005302.2 transcript:ENSORLT00000006686.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 1.021e-9
Identity = 29/85 (34.12%), Postives = 43/85 (50.59%), Query Frame = 3
Query:  114 SGFK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFF-----FDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
            +G K +NS+L  ++++E    +  IA+KV P+   P  +   ++F       D CSNIFL  V LH   G V    LH   H+ I
Sbjct:   19 AGLKQQNSHLTQVEVDEMLRLVGHIATKVPPNNAVPCRVVFLVKFLDPTYLLDICSNIFLDIVLLHGLHGTVHCILLHFIRHVCI 103          
BLAST of SMED30033197 vs. Ensembl Medaka
Match: ENSORLT00000029240.1 (pep primary_assembly:ASM223467v1:13:10253300:10255192:1 gene:ENSORLG00000024686.1 transcript:ENSORLT00000029240.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.311e-9
Identity = 26/78 (33.33%), Postives = 42/78 (53.85%), Query Frame = 3
Query:  120 FK*KNSNLAXIKINEXFCFMXDIASKVSPHXTXPGWIG*FIEFFFDKCSNIFLYXVFLHRCRGXVDSSFLHIFLHISI 353
             K +N  LA +++N+  C + D+A++VS +   PG +   +       SN+ LY VFLH   G V    LH+  H+S+
Sbjct:    5 LKEQNGYLAQVEVNKMLCLVGDVAAEVSANDAVPGRVV-LLVELLKLGSNVLLYVVFLHGLCGTVHGILLHLLGHVSV 81          
BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000073811.1 (SMESG000073811.1)

HSP 1 Score: 149.828 bits (377), Expect = 2.271e-48
Identity = 72/80 (90.00%), Postives = 72/80 (90.00%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADMQEDMQEAAVN ATTAMEKY IEKDIAAFIKKEFDKL NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMQEDMQEAAVNCATTAMEKYNIEKDIAAFIKKEFDKLYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000005286.1 (SMESG000005286.1)

HSP 1 Score: 135.961 bits (341), Expect = 8.179e-43
Identity = 64/80 (80.00%), Postives = 69/80 (86.25%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM ++MQEAA+N AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   10 NADMTDEMQEAAINYATQAMEKFNIEKDIAAYIKKEFDKSFNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89          
BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000043518.1 (SMESG000043518.1)

HSP 1 Score: 133.265 bits (334), Expect = 9.568e-42
Identity = 64/80 (80.00%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM EDMQ+ AV  AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   12 NADMSEDMQQDAVECATQAMEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 91          
BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000003543.1 (SMESG000003543.1)

HSP 1 Score: 132.109 bits (331), Expect = 2.958e-41
Identity = 62/80 (77.50%), Postives = 67/80 (83.75%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A+  AT AMEK+ IEKDIAA+IKKEFDK  NPTW C VGRNFGSYV HETK FIYFY GQVAILLFKSG
Sbjct:   12 NADMADDMQQEAIECATQAMEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG 91          
BLAST of SMED30033197 vs. Planmine SMEST
Match: SMESG000017229.1 (SMESG000017229.1)

HSP 1 Score: 128.642 bits (322), Expect = 5.865e-40
Identity = 59/80 (73.75%), Postives = 66/80 (82.50%), Query Frame = -3
Query:  114 NADMQEDMQEAAVNXATTAMEKYXIEKDIAAFIKKEFDKLSNPTWXCXVGRNFGSYVXHETKXFIYFYXGQVAILLFKSG 353
            NADM +DMQ+ A++ A  AMEKY +EKDIAA+IKKEFDK  NPTW   VGRNFGSYV HETK FIYFY GQVA+LLFKSG
Sbjct:   10 NADMSDDMQQEAIDVALQAMEKYNVEKDIAAYIKKEFDKKYNPTWHAIVGRNFGSYVTHETKHFIYFYLGQVAVLLFKSG 89          
The following BLAST results are available for this feature:
BLAST of SMED30033197 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DYNLL21.678e-4281.25dynein light chain LC8-type 2 [Source:HGNC Symbol;... [more]
DYNLL13.566e-4077.50dynein light chain LC8-type 1 [Source:HGNC Symbol;... [more]
DYNLL13.566e-4077.50dynein light chain LC8-type 1 [Source:HGNC Symbol;... [more]
DYNLL13.566e-4077.50dynein light chain LC8-type 1 [Source:HGNC Symbol;... [more]
DYNLL13.566e-4077.50dynein light chain LC8-type 1 [Source:HGNC Symbol;... [more]
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BLAST of SMED30033197 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dlc-11.107e-4177.50Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.107e-4177.50Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.107e-4177.50Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-59.959e-1637.50Dynein Light Chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
dlc-23.894e-1334.18Probable dynein light chain 2, cytoplasmic [Sourc... [more]
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BLAST of SMED30033197 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cdlc29.465e-4278.75gene:FBgn0026141 transcript:FBtr0077924[more]
Cdlc29.465e-4278.75gene:FBgn0026141 transcript:FBtr0305553[more]
Cdlc29.465e-4278.75gene:FBgn0026141 transcript:FBtr0077925[more]
ctp2.652e-4177.50gene:FBgn0011760 transcript:FBtr0070706[more]
ctp2.652e-4177.50gene:FBgn0011760 transcript:FBtr0070704[more]
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BLAST of SMED30033197 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dynll2a1.115e-4281.25dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:... [more]
dynll2b1.115e-4281.25dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:... [more]
dynll2a1.115e-4281.25dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:... [more]
dynll12.987e-4178.75dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z... [more]
dynll12.987e-4178.75dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z... [more]
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BLAST of SMED30033197 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
dynll21.521e-4281.25dynein light chain LC8-type 2 [Source:NCBI gene;Ac... [more]
dynll13.857e-4178.75dynein light chain LC8-type 1 [Source:NCBI gene;Ac... [more]
ENSXETT00000015639.15.979e-1343.21pep primary_assembly:Xenopus_tropicalis_v9.1:2:489... [more]
rnf1813.321e-833.75ring finger protein 181 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of SMED30033197 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dynll21.152e-4281.25dynein light chain LC8-type 2 [Source:MGI Symbol;A... [more]
Dynll21.152e-4281.25dynein light chain LC8-type 2 [Source:MGI Symbol;A... [more]
Dynll21.152e-4281.25dynein light chain LC8-type 2 [Source:MGI Symbol;A... [more]
Dynll12.448e-4077.50dynein light chain LC8-type 1 [Source:MGI Symbol;A... [more]
Dynll12.448e-4077.50dynein light chain LC8-type 1 [Source:MGI Symbol;A... [more]
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BLAST of SMED30033197 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q78P75|DYL2_RAT8.058e-4281.25Dynein light chain 2, cytoplasmic OS=Rattus norveg... [more]
sp|Q9D0M5|DYL2_MOUSE8.058e-4281.25Dynein light chain 2, cytoplasmic OS=Mus musculus ... [more]
sp|Q96FJ2|DYL2_HUMAN8.058e-4281.25Dynein light chain 2, cytoplasmic OS=Homo sapiens ... [more]
sp|Q3MHR3|DYL2_BOVIN8.058e-4281.25Dynein light chain 2, cytoplasmic OS=Bos taurus OX... [more]
sp|O96860|DYL2_DROME9.488e-4178.75Dynein light chain 2, cytoplasmic OS=Drosophila me... [more]
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BLAST of SMED30033197 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V9L8Z18.372e-4082.50Dynein light chain (Fragment) OS=Callorhinchus mil... [more]
A0A4W3IBH79.004e-4082.50Dynein light chain OS=Callorhinchus milii OX=7868 ... [more]
A0A401QGE49.004e-4082.50Dynein light chain OS=Scyliorhinus torazame OX=757... [more]
A0A3B5K1U39.304e-4082.50Dynein light chain OS=Takifugu rubripes OX=31033 G... [more]
A0A3Q3MX839.304e-4082.50Dynein light chain OS=Mastacembelus armatus OX=205... [more]
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BLAST of SMED30033197 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000047143.19.451e-4381.25dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
dynll2b9.451e-4381.25dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
dynll11.336e-3376.06dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z... [more]
ENSAMXT00000013314.24.726e-927.71pep primary_assembly:Astyanax_mexicanus-2.0:1:2333... [more]
dnal4b7.816e-933.75dynein axonemal light chain 4 [Source:NCBI gene;Ac... [more]
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BLAST of SMED30033197 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033197 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
DYN21.525e-2042.31Cytoplasmic light chain dynein, microtubule motor ... [more]
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BLAST of SMED30033197 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO445821.634e-4178.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385572.697e-934.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30033197 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000005747.24.809e-4280.00dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
dynll15.425e-4280.00dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
DYNLL21.932e-4076.25dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
ENSORLT00000006686.21.021e-934.12pep primary_assembly:ASM223467v1:14:12601680:12602... [more]
ENSORLT00000029240.12.311e-933.33pep primary_assembly:ASM223467v1:13:10253300:10255... [more]
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BLAST of SMED30033197 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000073811.12.271e-4890.00SMESG000073811.1[more]
SMESG000005286.18.179e-4380.00SMESG000005286.1[more]
SMESG000043518.19.568e-4280.00SMESG000043518.1[more]
SMESG000003543.12.958e-4177.50SMESG000003543.1[more]
SMESG000017229.15.865e-4073.75SMESG000017229.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033197 ID=SMED30033197|Name=SMED30033197|organism=Schmidtea mediterranea sexual|type=transcript|length=355bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTCAATATATAT
TTATTAAAATCCTAACAAAAAACTCTGATAAAAATCAAAATTTTATTTTA
TGGAATAAAATTATCCGGATTTAAATAAAAGAATAGCAACTTGGCCCANA
TAAAAATAAATGAAANGTTTTGTTTCATGAGNGACATAGCTTCCAAAGTT
TCTCCCCACANTACAGNGCCAGGTTGGATTGGATAATTTATCGAATTCTT
TTTTGATAAATGCAGCAATATCTTTCTCTATGNTGTATTTCTCCATCGCT
GTCGTGGCNCAGTTGACAGCAGCTTCCTGCATATCTTCTTGCATATCAGC
ATTTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR037177DLC_sf
IPR019763Dynein_light_1/2_CS
IPR001372Dynein_light_chain_typ-1/2
Vocabulary: cellular component
TermDefinition
GO:0005875microtubule associated complex
GO:0030286dynein complex
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001372Dynein light chain, type 1/2SMARTSM01375Dynein_light_2coord: 1..79
e-value: 4.1E-49
score: 179.2
IPR001372Dynein light chain, type 1/2PFAMPF01221Dynein_lightcoord: 1..79
e-value: 1.3E-39
score: 134.3
IPR001372Dynein light chain, type 1/2PANTHERPTHR11886DYNEIN LIGHT CHAINcoord: 1..80
IPR037177Dynein light chain superfamilyGENE3DG3DSA:3.30.740.10coord: 1..80
e-value: 1.3E-43
score: 149.0
IPR037177Dynein light chain superfamilySUPERFAMILYSSF54648DLCcoord: 1..80
NoneNo IPR availablePANTHERPTHR11886:SF62DYNEIN LIGHT CHAINcoord: 1..80
IPR019763Dynein light chain, type 1/2, conserved sitePROSITEPS01239DYNEIN_LIGHT_1coord: 46..60