DNA topoisomerase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033081 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3B (DNA topoisomerase III beta [Source:HGNC Symbol;Acc:HGNC:11993]) HSP 1 Score: 552.362 bits (1422), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 322/426 (75.59%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + +VLWLDCD+EGENICFEVLD++ V +K + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V ++SL L+W+R R+FD+ +A +FL +TK E A+V + S KE K RP ALNTVEMLR ASS LG+GP M AE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAG-----QPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 409.068 bits (1050), Expect = 0.000e+0 Identity = 197/397 (49.62%), Postives = 277/397 (69.77%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT +G+ ++ G+ VMPW + + +LP +G P+ + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ VF KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC SG+L+LD ++ P++K+ CN+C V+ + V V + +C +C + L + N +P D GCVFCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-ELFTCSGKTVLSPGFTEVMPWQSVPLEESLPT-CQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3B (DNA topoisomerase III beta [Source:HGNC Symbol;Acc:HGNC:11993]) HSP 1 Score: 552.362 bits (1422), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 322/426 (75.59%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + +VLWLDCD+EGENICFEVLD++ V +K + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V ++SL L+W+R R+FD+ +A +FL +TK E A+V + S KE K RP ALNTVEMLR ASS LG+GP M AE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAG-----QPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 409.068 bits (1050), Expect = 0.000e+0 Identity = 197/397 (49.62%), Postives = 277/397 (69.77%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT +G+ ++ G+ VMPW + + +LP +G P+ + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ VF KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC SG+L+LD ++ P++K+ CN+C V+ + V V + +C +C + L + N +P D GCVFCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-ELFTCSGKTVLSPGFTEVMPWQSVPLEESLPT-CQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3B (DNA topoisomerase III beta [Source:HGNC Symbol;Acc:HGNC:11993]) HSP 1 Score: 333.954 bits (855), Expect = 1.752e-171 Identity = 159/291 (54.64%), Postives = 215/291 (73.88%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSH 2232
DC+ S I+ +G E FT +G+ ++ G+ VMPW + + +LP +G P+ + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ VF KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ +CSH
Sbjct: 218 DCKYLQSTISFRIGP-ELFTCSGKTVLSPGFTEVMPWQSVPLEESLPT-CQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKK----GECSH 502 HSP 2 Score: 291.197 bits (744), Expect = 1.752e-171 Identity = 137/215 (63.72%), Postives = 166/215 (77.21%), Query Frame = 3
Query: 675 LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIV-SEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V ++SL L+W+R R+FD+ +A +FL +TK E A+V + S KE K RP ALNTVEMLR ASS LG+GP M AE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 2 LDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 216
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3B (DNA topoisomerase III beta [Source:HGNC Symbol;Acc:HGNC:11993]) HSP 1 Score: 338.191 bits (866), Expect = 1.954e-109 Identity = 166/251 (66.14%), Postives = 194/251 (77.29%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQ 794
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + +VLWLDCD+EGENICFEVLD++ V +K + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAG-----QPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAK 246
BLAST of DNA topoisomerase vs. Ensembl Human
Match: TOP3A (DNA topoisomerase III alpha [Source:HGNC Symbol;Acc:HGNC:11992]) HSP 1 Score: 288.886 bits (738), Expect = 9.823e-105 Identity = 168/424 (39.62%), Postives = 237/424 (55.90%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
++KV VAEK A IA LS + +G + ++E++ H + V MTSV GH+++ +F ++ W + +P+ LF A K + + L++E +V+W DCDREGENI FE++ + V KP ++LRA+FS IT +R A NL P++ + +VD RQELDLRIG AFTRFQT Q + + L +LIS+G CQ PTLGF VER+ IQ+F PE F +K+ + ++ W R R+F+ + L + A VV + K K RPQAL+TVE+ + AS L I M IAEKLYTQG+ISYPRTET + +L ++ QT P WG +S+L+ G PR G + HPPI P + L GD QRLY++I RH++A S
Sbjct: 32 GVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFD-----YHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAV--KPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNN--LQGDEQRLYEFIVRHFLACCS 446 HSP 2 Score: 113.62 bits (283), Expect = 9.823e-105 Identity = 73/233 (31.33%), Postives = 108/233 (46.35%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKLITSTPQRLHCG-----NCRSTCSLP 2058
A E F G ++ + YL V P+ D LP QG ST + +G+T PP LTE++LI+ MEKHGIGTDA+ HI+ I R YV + ++ P LG+ LV GY + E+ P +R+ +E L LI G + V+R + + F V ++E F + + + P+ +C +CN+ M L T + CRS LP
Sbjct: 461 AQERFVAHGLMILARNYLDVYPYDHWSDKILPV-YEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLP 692
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 486.108 bits (1250), Expect = 0.000e+0 Identity = 240/423 (56.74%), Postives = 300/423 (70.92%), Query Frame = 3
Query: 66 VFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-----CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS--LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATV 1313
V MVAEKP LA SIA LS Q KG+NGVCSV E++G F +GR R+K+TS CGHVMS++F K+NNW+ VDP EL+SA T + EANPKMKM FL EA ++ +VLWLDCD+EGENICFEV+D+++ K + RA FSAIT+KD++ AM NL P++ + SVDARQELDLRIGC+FTRFQTK+FQGKYGDLDS +IS+GPCQTPTLGFCV R+D+I FKPE +WVLK S+ EW RGRIFD VA +F+ K T V+ +S KE K RP ALNTVE++R ASS LG+ PS M +AE LYTQG+ISYPRTETT YS +FDL L+ +WG++V +L G RP+ G+D GDHPPITPMRP G LSGD R+Y+Y+++H++AT+
Sbjct: 5 VLMVAEKPLLADSIANLLSNGQAKKRKGWNGVCSVSEYDGQF---NGRA--ARFKVTSTCGHVMSLDFPPKFNNWERVDPAELYSAPTQRIEANPKMKMNEFLASEAKNADFLVLWLDCDKEGENICFEVIDAVKCAMFKNRQDFMSDVYRAHFSAITEKDIKAAMRNLGRPDQNMSHSVDARQELDLRIGCSFTRFQTKFFQGKYGDLDSNVISYGPCQTPTLGFCVTRHDQIVQFKPEQYWVLKTNFTTSDDGQIFSPEWQRGRIFDAEVARVFVDRIKKCHTGLVLDVSKKEARKERPCALNTVELMRVASSSLGMSPSTTMHVAEALYTQGYISYPRTETTAYSANFDLTGTLKILAGDRKWGSVVSQVLADGIKRPKSGVDKGDHPPITPMRP-NHGGLSGDMSRIYEYVSQHFVATL 421 HSP 2 Score: 350.903 bits (899), Expect = 0.000e+0 Identity = 178/421 (42.28%), Postives = 267/421 (63.42%), Query Frame = 1
Query: 1354 LMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQ-GQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLP--AGGTVRQYKQVRCPLDGFELVVWSQGTKGK---SFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLST-SCDLCSSQHLQLKLNTKDNPNS---------DDMFSGCVFCD--KKMCDLMQLEHASFRNNS 2559
LM C EV+ + M G E FTL G+ +++ G+ AVMPWL ++++ ++P + Q G + + ++ +T PP +LTE+ELI+ MEKHGIGTDASIPVHI I +RNYV + +GR++ PT+LG LV GY ++D ELVLP MR+ +EEQL+L+A G A++ DV H + +F KF+YFV NI ++ LFE +F++L+D+GKP +RCGKC RYMKL+ + PQRL+C C+ T ++P G ++ +CPLD F+++++ QG GK +F LCP+C+ NPPFE M + +GC+ C HP C S + V C + G++LLD + P++++ CN+CP ++ + + V V ST SC+ C +Q ++ + PNS + GC+FCD KK D + L HA S
Sbjct: 421 LMKQCVYEVTTVKMSCG-DEQFTLQGKNVVEPGFTAVMPWLSVEEECSVPKSLQQIGAKIELQNAEIQARETTPPGYLTEAELITLMEKHGIGTDASIPVHINTITQRNYVTVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNIPAVDTLFEASFTTLSDTGKPFSRCGKCRRYMKLVQTKPQRLYCPTCQETYAVPNFKDGVLKVIGDHKCPLDDFDVILY-QGPGGKLARTFTLCPFCFNNPPFEKMPQGAGCDTCVHPSCQYSISSNGVCGCLQDCGGVMLLDTQSHPKWRMTCNKCPSVVGLFEGAIKVKVDSTRSCEQCDAQFIKAEF----KPNSGKPGAIEYESSSYEGCIFCDAMKKYPDAINLNHAYLTEES 835
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 350.903 bits (899), Expect = 0.000e+0 Identity = 178/421 (42.28%), Postives = 267/421 (63.42%), Query Frame = 1
Query: 1354 LMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQ-GQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLP--AGGTVRQYKQVRCPLDGFELVVWSQGTKGK---SFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLST-SCDLCSSQHLQLKLNTKDNPNS---------DDMFSGCVFCD--KKMCDLMQLEHASFRNNS 2559
LM C EV+ + M G E FTL G+ +++ G+ AVMPWL ++++ ++P + Q G + + ++ +T PP +LTE+ELI+ MEKHGIGTDASIPVHI I +RNYV + +GR++ PT+LG LV GY ++D ELVLP MR+ +EEQL+L+A G A++ DV H + +F KF+YFV NI ++ LFE +F++L+D+GKP +RCGKC RYMKL+ + PQRL+C C+ T ++P G ++ +CPLD F+++++ QG GK +F LCP+C+ NPPFE M + +GC+ C HP C S + V C + G++LLD + P++++ CN+CP ++ + + V V ST SC+ C +Q ++ + PNS + GC+FCD KK D + L HA S
Sbjct: 285 LMKQCVYEVTTVKMSCG-DEQFTLQGKNVVEPGFTAVMPWLSVEEECSVPKSLQQIGAKIELQNAEIQARETTPPGYLTEAELITLMEKHGIGTDASIPVHINTITQRNYVTVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNIPAVDTLFEASFTTLSDTGKPFSRCGKCRRYMKLVQTKPQRLYCPTCQETYAVPNFKDGVLKVIGDHKCPLDDFDVILY-QGPGGKLARTFTLCPFCFNNPPFEKMPQGAGCDTCVHPSCQYSISSNGVCGCLQDCGGVMLLDTQSHPKWRMTCNKCPSVVGLFEGAIKVKVDSTRSCEQCDAQFIKAEF----KPNSGKPGAIEYESSSYEGCIFCDAMKKYPDAINLNHAYLTEES 699 HSP 2 Score: 334.339 bits (856), Expect = 0.000e+0 Identity = 159/275 (57.82%), Postives = 199/275 (72.36%), Query Frame = 3
Query: 495 ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS--LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATV 1313
+ RA FSAIT+KD++ AM NL P++ + SVDARQELDLRIGC+FTRFQTK+FQGKYGDLDS +IS+GPCQTPTLGFCV R+D+I FKPE +WVLK S+ EW RGRIFD VA +F+ K T V+ +S KE K RP ALNTVE++R ASS LG+ PS M +AE LYTQG+ISYPRTETT YS +FDL L+ +WG++V +L G RP+ G+D GDHPPITPMRP G LSGD R+Y+Y+++H++AT+
Sbjct: 12 VYRAHFSAITEKDIKAAMRNLGRPDQNMSHSVDARQELDLRIGCSFTRFQTKFFQGKYGDLDSNVISYGPCQTPTLGFCVTRHDQIVQFKPEQYWVLKTNFTTSDDGQIFSPEWQRGRIFDAEVARVFVDRIKKCHTGLVLDVSKKEARKERPCALNTVELMRVASSSLGMSPSTTMHVAEALYTQGYISYPRTETTAYSANFDLTGTLKILAGDRKWGSVVSQVLADGIKRPKSGVDKGDHPPITPMRP-NHGGLSGDMSRIYEYVSQHFVATL 285
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: top-3 (DNA topoisomerase 3 [Source:UniProtKB/Swiss-Prot;Acc:O61660]) HSP 1 Score: 268.085 bits (684), Expect = 7.374e-94 Identity = 150/427 (35.13%), Postives = 231/427 (54.10%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVS-FLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDL-----DSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M++ VAEK +A +A LS +G + ++ N +Q +TSV GH+M+ F+ +NW +ELF A ++ P+MK++ L+++A +I+V+W DCDREGE I E++ + +++ I RA+FS IT + A NL ++ +VD R ELDLRIG AFTR QT + + ++ DL S++IS+G CQ PTLGF +RY I++F E FW L ++ ++ W+R R+FD+ ++ K A V ++ K K RPQAL+TVE+ + S L + M +AEKLY++GFISYPRTET + +L +Q QT WG +L G PR G + + HPPI P++ + +L GD ++Y+ + RH++A VS
Sbjct: 1 MKRALFVAEKNDVAKGVAAILSNGTANRREGRSKFNKIYTLNTELFG-----QQTAISVTSVSGHMMNFQFHENMSNWQTASMVELFRAP-VRHVVTPEMKLIEQTLREQAQRHDILVVWTDCDREGEAIGAEIV-KVCRDSNRRLDIFRARFSEITKAAITRAARNLIRLDEKTVAAVDCRSELDLRIGSAFTRLQTLHLRNRFRDLLGQNDTSQVISYGSCQFPTLGFVTDRYKMIENFVSEPFWKLIVEHTRESHKVEFLWDRNRLFDRDTVDILHDECKETKEAHVEKVAKKPKSKWRPQALDTVELEKLGISKLRMSAKQTMQVAEKLYSKGFISYPRTETNKFPAGLNLTPLVQQQTQSNIWGDFANEVLQNG-VNPRNGRKSDEAHPPIHPLKFTEKHQLQGDDWKVYELVVRHFLACVS 419 HSP 2 Score: 97.0561 bits (240), Expect = 7.374e-94 Identity = 59/172 (34.30%), Postives = 92/172 (53.49%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKF 1878
D + E + + + VG +E F +G R+ D GYL V + + LP T+G+ + +G+T PDFLTE++LIS M+K+GIGTDA+ HI+ I R Y+ + K+ P+ LG+ LV GY + + P +R+ +E L I G +V+ I + A F
Sbjct: 421 DAQGEETMVNLTVG-TEKFHASGLRIRDMGYLKVYVYEKWGNRLLPT-YTEGERFTDFELKIGDGKTQAPDFLTEADLISLMDKYGIGTDATHAEHIEKIKTREYIGVRPDGKLIPSFLGLALVDGYDDMGFAMSKPDLRANLEIGLKEICDGRRQKQEVLDEQIGKYRAIF 590
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 293.123 bits (749), Expect = 5.309e-91 Identity = 147/335 (43.88%), Postives = 214/335 (63.88%), Query Frame = 1
Query: 1606 MEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLP--AGGTVRQYKQVRCPLDGFELVVWSQGTKGK---SFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLST-SCDLCSSQHLQLKLNTKDNPNS---------DDMFSGCVFCD--KKMCDLMQLEHASFRNNS 2559
MEKHGIGTDASIPVHI I +RNYV + +GR++ PT+LG LV GY ++D ELVLP MR+ +EEQL+L+A G A++ DV H + +F KF+YFV NI ++ LFE +F++L+D+GKP +RCGKC RYMKL+ + PQRL+C C+ T ++P G ++ +CPLD F+++++ QG GK +F LCP+C+ NPPFE M + +GC+ C HP C S + V C + G++LLD + P++++ CN+CP ++ + + V V ST SC+ C +Q ++ + PNS + GC+FCD KK D + L HA S
Sbjct: 1 MEKHGIGTDASIPVHINTITQRNYVTVESGRRLVPTRLGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKADYLDVKNHALKMFEMKFQYFVTNIPAVDTLFEASFTTLSDTGKPFSRCGKCRRYMKLVQTKPQRLYCPTCQETYAVPNFKDGVLKVIGDHKCPLDDFDVILY-QGPGGKLARTFTLCPFCFNNPPFEKMPQGAGCDTCVHPSCQYSISSNGVCGCLQDCGGVMLLDTQSHPKWRMTCNKCPSVVGLFEGAIKVKVDSTRSCEQCDAQFIKAEF----KPNSGKPGAIEYESSSYEGCIFCDAMKKYPDAINLNHAYLTEES 330
BLAST of DNA topoisomerase vs. Ensembl Celegans
Match: Y48C3A.14 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:H1UBJ8]) HSP 1 Score: 68.1662 bits (165), Expect = 4.837e-13 Identity = 42/133 (31.58%), Postives = 67/133 (50.38%), Query Frame = 1
Query: 2197 MQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLST-SCDLCSSQHLQLKLNTKDNPNS---------DDMFSGCVFCD--KKMCDLMQLEHASFRNNS 2559
M + +GC+ C HP C S + V C + G++LLD + P++++ CN+CP ++ + + V V ST SC+ C +Q ++ + PNS + GC+FCD KK D + L HA S
Sbjct: 1 MPQGAGCDTCVHPSCQYSISSNGVCGCLQDCGGVMLLDTQSHPKWRMTCNKCPSVVGLFEGAIKVKVDSTRSCEQCDAQFIKAEF----KPNSGKPGAIEYESSSYEGCIFCDAMKKYPDAINLNHAYLTEES 129
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3beta (gene:FBgn0026015 transcript:FBtr0340028) HSP 1 Score: 489.96 bits (1260), Expect = 0.000e+0 Identity = 239/423 (56.50%), Postives = 299/423 (70.69%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK---ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA S+A LS +CT +G CS HEW G F N G V ++MTSVCGHVMS++FN KYN WD VDPI+LF +T K+E NPK M FL EA G + +VLWLDCD+EGENICFEV+D++++V + RA FSAIT+KD++ AM L +PN+ EA SVDARQELDLRIGCAFTRFQTK+FQ +YGDLDS LIS+GPCQTPTLGFCV+R+D+IQ+FKPE+FW L Q++ + + LEW RGR+F K +A + L K A V S++ KE K +PQALNTVE++R SSGLGIGP M IAE+LYTQG+ISYPRTET Y +FDL A L ++G RS+L PRKG DAGDHPPITPM+ + + D+ R+Y++I RH++ TVS
Sbjct: 1 MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNF-RNEG---SVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL--QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVS 416 HSP 2 Score: 377.867 bits (969), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 251/397 (63.22%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSD--DMFSGCVFCDKKMCDLMQLEHA 2541
D + V+ + VG E F+ T +ID G+ VM W +D+ P QG + I+ L E QTGPPD+LTESELI+ ME+HGIGTDASIPVHI NI +RNYV I GRK+ PT LGIVLVHGYQ+ID ELVLP MR+ VE L LIA+G AN DV+RH I +F KF YFV+NI M+ LFE +FS LA+SGK +RCGKC RYMK I + P RLHC +C T +LP G V+ Y++ +CPLD F+L+ +S G KG+S+ CPYCY +PPF DM GCN C++ CP S L +++C ECP+G+L+LD + AP +KL CN C V+I + V C C +Q + + + + D + SGC+FC L++ A
Sbjct: 418 DLKYRVTTAKLSVGM-ETFSCTASVLIDAGFTKVMTWSAFGKDEPQPP--FVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPI-GNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRA 810
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3beta (gene:FBgn0026015 transcript:FBtr0070930) HSP 1 Score: 489.96 bits (1260), Expect = 0.000e+0 Identity = 239/423 (56.50%), Postives = 299/423 (70.69%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK---ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA S+A LS +CT +G CS HEW G F N G V ++MTSVCGHVMS++FN KYN WD VDPI+LF +T K+E NPK M FL EA G + +VLWLDCD+EGENICFEV+D++++V + RA FSAIT+KD++ AM L +PN+ EA SVDARQELDLRIGCAFTRFQTK+FQ +YGDLDS LIS+GPCQTPTLGFCV+R+D+IQ+FKPE+FW L Q++ + + LEW RGR+F K +A + L K A V S++ KE K +PQALNTVE++R SSGLGIGP M IAE+LYTQG+ISYPRTET Y +FDL A L ++G RS+L PRKG DAGDHPPITPM+ + + D+ R+Y++I RH++ TVS
Sbjct: 1 MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNF-RNEG---SVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL--QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVS 416 HSP 2 Score: 377.867 bits (969), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 251/397 (63.22%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSD--DMFSGCVFCDKKMCDLMQLEHA 2541
D + V+ + VG E F+ T +ID G+ VM W +D+ P QG + I+ L E QTGPPD+LTESELI+ ME+HGIGTDASIPVHI NI +RNYV I GRK+ PT LGIVLVHGYQ+ID ELVLP MR+ VE L LIA+G AN DV+RH I +F KF YFV+NI M+ LFE +FS LA+SGK +RCGKC RYMK I + P RLHC +C T +LP G V+ Y++ +CPLD F+L+ +S G KG+S+ CPYCY +PPF DM GCN C++ CP S L +++C ECP+G+L+LD + AP +KL CN C V+I + V C C +Q + + + + D + SGC+FC L++ A
Sbjct: 418 DLKYRVTTAKLSVGM-ETFSCTASVLIDAGFTKVMTWSAFGKDEPQPP--FVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPI-GNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRA 810
BLAST of DNA topoisomerase vs. Ensembl Fly
Match: Top3alpha (gene:FBgn0040268 transcript:FBtr0081246) HSP 1 Score: 290.426 bits (742), Expect = 1.034e-97 Identity = 167/423 (39.48%), Postives = 234/423 (55.32%), Query Frame = 3
Query: 63 KVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVAN--LFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
K VAEK A +IA LS +G++ V ++ P R + + MTSV GH+M + F Y NW VDP LF A K + + L++E G +++W DCDREGENI +E++D + KP + RA FS IT +R A+ L P+K ++ +VD R ELDLR G A TRFQT Q + + + KLIS+G CQ PTLGF ERY EI++F E FW +K+ + + +++ W R R+FDK L L L + + A V S++VK K RP L+TVEM + S L + M+IAEKLYT+GFISYPRTET +S F L ++ QT H +WG + +++ G PR G + HPPI P + A L G+ R+Y+ + RH++A VS
Sbjct: 27 KYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPV-----RGQNAKMVMTSVSGHMMQLAFQVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCRAI--KPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRLQRLFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARNRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWG-PNPRNGNKSDQAHPPIHPTKLAEN--LQGNEARVYELVVRHFLACVS 439 HSP 2 Score: 88.1965 bits (217), Expect = 1.034e-97 Identity = 54/169 (31.95%), Postives = 83/169 (49.11%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMN 1911
A E FT G + ++ YL V + + GQ + SL EG T P LTE++LI+ MEKHGIGTDA+ HI I ER Y+ ++ + P +G+ L GY ++ L P +R+ E L LI +G + V+ I + ++ + I+ M+
Sbjct: 454 AGEKFTANGLVIHERNYLDVYVYDKWSAKQI-HHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQAYQQITDKITAMD 621
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3b (DNA topoisomerase III beta [Source:ZFIN;Acc:ZDB-GENE-070705-37]) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 277/426 (65.02%), Postives = 324/426 (76.06%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS C+T KG NG CSVHE+ G FT + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +KP + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPE +WV++ +V + S L L+W+R R+FD+ + +F+ + K A V S++ KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N WG V++LL G RPRKG DAGDHPPITPMR A + EL D RLYDYI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSIAKILSKGSCSTRKGLNGACSVHEYMGSFTG-----QNVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKPYGNERTVYRAKFSSITDTDICNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPEAYWVVQAKVFKGKDSPLTLDWDRVRVFDRDIGQMFVNMAKTAKEARVDSVTKKEKAKQRPLALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQANSTFWGETVKALLSEGINRPRKGADAGDHPPITPMRAASENELGSDGWRLYDYITRHFIATVS 421 HSP 2 Score: 417.157 bits (1071), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 278/401 (69.33%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
PDC+ + I+ + A+E FT +G+ +I GY VMPW + + LP +G+M + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GRK++PT LGIVLVHGY +IDA+LVLP +RSAVE+QL LIA G AN V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK+++CPLD FELV+W+ G++GKS+ +CPYC++NPPF DM++ GCN+C+HP C S L + C EC +G+L+ D ++ P++++ CN+C V++ V V SCD C + + + N + P+ + +GCVFCD DL++L+HA+ R+
Sbjct: 422 PDCKYLQTTISFSI-ATEGFTCSGKTLISAGYTEVMPWQGIAQEEALPV-CERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKFHYFVDSIAGMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELKCPLDEFELVLWTSGSRGKSYPVCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECETGVLVFDPTSGPKWRMACNKCNVVVHFFEQAHKVQVSQDSCDSCEASLVIVDFNKTRSPLPDGETQHTGCVFCDSAFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3b (DNA topoisomerase III beta [Source:ZFIN;Acc:ZDB-GENE-070705-37]) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 277/426 (65.02%), Postives = 324/426 (76.06%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS C+T KG NG CSVHE+ G FT + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +KP + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPE +WV++ +V + S L L+W+R R+FD+ + +F+ + K A V S++ KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N WG V++LL G RPRKG DAGDHPPITPMR A + EL D RLYDYI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSIAKILSKGSCSTRKGLNGACSVHEYMGSFTG-----QNVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKPYGNERTVYRAKFSSITDTDICNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPEAYWVVQAKVFKGKDSPLTLDWDRVRVFDRDIGQMFVNMAKTAKEARVDSVTKKEKAKQRPLALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQANSTFWGETVKALLSEGINRPRKGADAGDHPPITPMRAASENELGSDGWRLYDYITRHFIATVS 421 HSP 2 Score: 417.157 bits (1071), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 278/401 (69.33%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
PDC+ + I+ + A+E FT +G+ +I GY VMPW + + LP +G+M + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GRK++PT LGIVLVHGY +IDA+LVLP +RSAVE+QL LIA G AN V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK+++CPLD FELV+W+ G++GKS+ +CPYC++NPPF DM++ GCN+C+HP C S L + C EC +G+L+ D ++ P++++ CN+C V++ V V SCD C + + + N + P+ + +GCVFCD DL++L+HA+ R+
Sbjct: 422 PDCKYLQTTISFSI-ATEGFTCSGKTLISAGYTEVMPWQGIAQEEALPV-CERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKFHYFVDSIAGMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELKCPLDEFELVLWTSGSRGKSYPVCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECETGVLVFDPTSGPKWRMACNKCNVVVHFFEQAHKVQVSQDSCDSCEASLVIVDFNKTRSPLPDGETQHTGCVFCDSAFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: BX005237.1 (DNA topoisomerase III beta [Source:NCBI gene;Acc:793414]) HSP 1 Score: 511.531 bits (1316), Expect = 0.000e+0 Identity = 246/374 (65.78%), Postives = 290/374 (77.54%), Query Frame = 3
Query: 213 EQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+ VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +KP + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPE +WV++ +V + S L L+W+R R+FD+ + +F+ + K A V S++ KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N WG V++LL G RPRKG DAGDHPPITPMR A + EL D RLYDYI RH+IATVS
Sbjct: 5 QNVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKPYGNERTVYRAKFSSITDTDICNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPEAYWVVQAKVFKGKDSPLTLDWDRVRVFDRDIGQMFVNMAKTAKEARVDSVTKKEKAKQRPLALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQANSTFWGETVKALLSEGINRPRKGADAGDHPPITPMRAASENELGSDGWRLYDYITRHFIATVS 378 HSP 2 Score: 416.772 bits (1070), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 278/401 (69.33%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
PDC+ + I+ + A+E FT +G+ +I GY VMPW + + LP +G+M + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GRK++PT LGIVLVHGY +IDA+LVLP +RSAVE+QL LIA G AN V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK+++CPLD FELV+W+ G++GKS+ +CPYC++NPPF DM++ GCN+C+HP C S L + C EC +G+L+ D ++ P++++ CN+C V++ V V SCD C + + + N + P+ + +GCVFCD DL++L+HA+ R+
Sbjct: 379 PDCKYLQTTISFSI-ATEGFTCSGKTLISAGYTEVMPWQGIAQEEALPV-CERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKFHYFVDSIAGMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELKCPLDEFELVLWTSGSRGKSYPVCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECETGVLVFDPTSGPKWRMACNKCNVVVHFFEQAHKVQVSQDSCDSCEASLVIVDFNKTRSPLPDGETQHTGCVFCDSAFQDLVELKHATMRH 777
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 286.189 bits (731), Expect = 1.443e-105 Identity = 168/440 (38.18%), Postives = 248/440 (56.36%), Query Frame = 3
Query: 18 KYFLYVYLNFIFNMQ--KVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQ-VRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKI-QVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+++ ++ ++ I Q KV VAEK A IA+ +S + +G CSV +N + + Q V MTSV GH++++ F + + W + P+ LF A K + + L++E +++W DCDREGENI FE++D + V KP ++ RA+FS IT +R A L P+ + +VD RQELDLRIG +FTRFQT Q + + L +LIS+G CQ PTLGF VER+ IQ+F PETF+ +K+ E+S++ W R R+F+ + + E A+V+S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + +L ++ QT EWG + +L++G PR G + HPPI P + L G+ +RLY++I RH++A S
Sbjct: 15 RFYRHISVSMIRRTQIKKVLCVAEKNDAAKGIAEIMSNGRSRRREG----CSV--YNKIYEYEYNLFGQNVTVNMTSVSGHLLALEFKAPFQKWHSCSPVLLFDAEVEKYCPENFIPIKRTLEREVRQCQALIVWTDCDREGENIGFEIIDVCKAV--KPNIQVFRARFSEITPNSIRRACETLTEPDINVSDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLSDQLISYGSCQFPTLGFVVERFKAIQAFIPETFFKIKVVHEPNEEESVEFNWKRHRLFNHTACLVLYQMCMEEPMAKVISVTSKPKSKWRPLPLDTVELEKLASRKLRISAKETMKIAEKLYTQGFISYPRTETNMFPQNLNLTRLVEQQTQDQEWGNFAQRILESGGPTPRNGNKSDQAHPPIHPTKYTNG--LQGNEKRLYEFIVRHFLACCS 444 HSP 2 Score: 118.627 bits (296), Expect = 1.443e-105 Identity = 68/208 (32.69%), Postives = 107/208 (51.44%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P+ P + + EGQT PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P +R+ +E L L+++G N A V+ + + + A F V ++E + + + P+ +C +CNR M L
Sbjct: 459 AEERFSASGLTIIARNYLEVYPYDKWYNKVIPSYSPNTTFQPTAI-EMVEGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKNKASVLSYHVAKYKAVFIESVRKAKKLDEALSNYLGPAQEIAEQEQVELEIPLPVRKCPQCNRDMVL 665
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: dus1l (dihydrouridine synthase 1-like [Source:Xenbase;Acc:XB-GENE-955162]) HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0 Identity = 276/426 (64.79%), Postives = 328/426 (77.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD+I V ++ + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+++V E L L+W+R R+FD+ +A +F+ +TK A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N+P W +V++LL G RPRKG DAGDHPPITPMR A + EL GD+ RLY+YI RH+IAT+S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSASSRKGLNGACSVHEYTGSFMG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLSMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAIHPVMNRAHGGERTVYRAKFSSITDTDILNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQVKVSRGQENPLILDWDRVRVFDREIAQMFINITKTAKEAQVESVSKKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNPFWADMVKTLLAEGINRPRKGTDAGDHPPITPMRSATEAELGGDAWRLYEYITRHFIATIS 421 HSP 2 Score: 389.037 bits (998), Expect = 4.557e-121 Identity = 180/349 (51.58%), Postives = 249/349 (71.35%), Query Frame = 1
Query: 1513 QGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHASFRN 2553
+G + L E QT PPD+LTE+ELIS ME+HGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +ID ELVLP +RSAVE+QL+LIA+G AN V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T SLP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY + PF DM++ GCN+C+HP C S + L + C EC SG+++LD ++ P++K+ CN+C V++ + V V + +CD C + + + N P D +GCVFCD DL++L+HA+ ++
Sbjct: 463 RGDKFSVDEMKLMEKQTCPPDYLTEAELISLMERHGIGTDASIPVHINNICQRNYVTVESGRRLKPTSLGIVLVHGYYKIDPELVLPTIRSAVEKQLNLIAQGKANFQQVLDHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHAPFRDMKKGMGCNECTHPTCQHSLSMLGIAQCVECESGVMVLDSTSGPKWKMSCNKCNVIVHFFENAHRVRVSAETCDACDAAVVHVDFNKAKCPLPADQTQHAGCVFCDPVFQDLVELKHATMKH 811
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: dus1l (dihydrouridine synthase 1-like [Source:Xenbase;Acc:XB-GENE-955162]) HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0 Identity = 276/426 (64.79%), Postives = 328/426 (77.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD+I V ++ + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+++V E L L+W+R R+FD+ +A +F+ +TK A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N+P W +V++LL G RPRKG DAGDHPPITPMR A + EL GD+ RLY+YI RH+IAT+S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSASSRKGLNGACSVHEYTGSFMG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLSMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAIHPVMNRAHGGERTVYRAKFSSITDTDILNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQVKVSRGQENPLILDWDRVRVFDREIAQMFINITKTAKEAQVESVSKKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNPFWADMVKTLLAEGINRPRKGTDAGDHPPITPMRSATEAELGGDAWRLYEYITRHFIATIS 421 HSP 2 Score: 405.601 bits (1041), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 274/401 (68.33%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I VG E FT T + +I G+ +MPW + +++ P +G + L E QT PPD+LTE+ELIS ME+HGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +ID ELVLP +RSAVE+QL+LIA+G AN V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T SLP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY + PF DM++ GCN+C+HP C S + L + C EC SG+++LD ++ P++K+ CN+C V++ + V V + +CD C + + + N P D +GCVFCD DL++L+HA+ ++
Sbjct: 423 DCKYLQTSIAFSVGP-EKFTTTCKEVISPGFTEIMPWQGIPKEESFQPC--ERGDKFSVDEMKLMEKQTCPPDYLTEAELISLMERHGIGTDASIPVHINNICQRNYVTVESGRRLKPTSLGIVLVHGYYKIDPELVLPTIRSAVEKQLNLIAQGKANFQQVLDHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHAPFRDMKKGMGCNECTHPTCQHSLSMLGIAQCVECESGVMVLDSTSGPKWKMSCNKCNVIVHFFENAHRVRVSAETCDACDAAVVHVDFNKAKCPLPADQTQHAGCVFCDPVFQDLVELKHATMKH 820
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: dus1l (dihydrouridine synthase 1-like [Source:Xenbase;Acc:XB-GENE-955162]) HSP 1 Score: 513.457 bits (1321), Expect = 0.000e+0 Identity = 258/426 (60.56%), Postives = 308/426 (72.30%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ VLD+I V ++ + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+++V E L L+W+R R+FD+ +A +F+ +TK A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N+P W +V++LL G RPRKG DAGDHPPITPMR A + EL GD+ RLY+YI RH+IAT+S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSASSRKGLNGACSVHEYTGSFMG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLSMVKFLQ-------------------------VLDAIHPVMNRAHGGERTVYRAKFSSITDTDILNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQVKVSRGQENPLILDWDRVRVFDREIAQMFINITKTAKEAQVESVSKKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNPFWADMVKTLLAEGINRPRKGTDAGDHPPITPMRSATEAELGGDAWRLYEYITRHFIATIS 396 HSP 2 Score: 406.371 bits (1043), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 274/401 (68.33%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I VG E FT T + +I G+ +MPW + +++ P +G + L E QT PPD+LTE+ELIS ME+HGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +ID ELVLP +RSAVE+QL+LIA+G AN V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T SLP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY + PF DM++ GCN+C+HP C S + L + C EC SG+++LD ++ P++K+ CN+C V++ + V V + +CD C + + + N P D +GCVFCD DL++L+HA+ ++
Sbjct: 398 DCKYLQTSIAFSVGP-EKFTTTCKEVISPGFTEIMPWQGIPKEESFQPC--ERGDKFSVDEMKLMEKQTCPPDYLTEAELISLMERHGIGTDASIPVHINNICQRNYVTVESGRRLKPTSLGIVLVHGYYKIDPELVLPTIRSAVEKQLNLIAQGKANFQQVLDHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHAPFRDMKKGMGCNECTHPTCQHSLSMLGIAQCVECESGVMVLDSTSGPKWKMSCNKCNVIVHFFENAHRVRVSAETCDACDAAVVHVDFNKAKCPLPADQTQHAGCVFCDPVFQDLVELKHATMKH 795
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: dus1l (dihydrouridine synthase 1-like [Source:Xenbase;Acc:XB-GENE-955162]) HSP 1 Score: 501.516 bits (1290), Expect = 0.000e+0 Identity = 250/425 (58.82%), Postives = 300/425 (70.59%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F E G + VVLWLDCD+EGENICFEVLD+I V ++ + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+++V E L L+W+R R+FD+ +A +F+ +TK A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N+P W +V++LL G RPRKG DAGDHPPITPMR A + EL GD+ RLY+YI RH+IAT+S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGNASSRKGLNGACSVHEYTGSFMG-----QTVRFKMTSVCGHVMSLDFIGSV---------------------------------EGRGCDYVVLWLDCDKEGENICFEVLDAIHPVMNRAHGGERTVYRAKFSSITDTDILNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQVKVSRGQENPLILDWDRVRVFDREIAQMFINITKTAKEAQVESVSKKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNPFWADMVKTLLAEGINRPRKGTDAGDHPPITPMRSATEAELGGDAWRLYEYITRHFIATIS 387 HSP 2 Score: 405.601 bits (1041), Expect = 0.000e+0 Identity = 195/401 (48.63%), Postives = 274/401 (68.33%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I VG E FT T + +I G+ +MPW + +++ P +G + L E QT PPD+LTE+ELIS ME+HGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +ID ELVLP +RSAVE+QL+LIA+G AN V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T SLP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY + PF DM++ GCN+C+HP C S + L + C EC SG+++LD ++ P++K+ CN+C V++ + V V + +CD C + + + N P D +GCVFCD DL++L+HA+ ++
Sbjct: 389 DCKYLQTSIAFSVGP-EKFTTTCKEVISPGFTEIMPWQGIPKEESFQPC--ERGDKFSVDEMKLMEKQTCPPDYLTEAELISLMERHGIGTDASIPVHINNICQRNYVTVESGRRLKPTSLGIVLVHGYYKIDPELVLPTIRSAVEKQLNLIAQGKANFQQVLDHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHAPFRDMKKGMGCNECTHPTCQHSLSMLGIAQCVECESGVMVLDSTSGPKWKMSCNKCNVIVHFFENAHRVRVSAETCDACDAAVVHVDFNKAKCPLPADQTQHAGCVFCDPVFQDLVELKHATMKH 786
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Match: cryba1 (crystallin beta A1 [Source:Xenbase;Acc:XB-GENE-956128]) HSP 1 Score: 282.722 bits (722), Expect = 1.837e-102 Identity = 159/423 (37.59%), Postives = 238/423 (56.26%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+ KV VAEK A IA +S ++ +GF+ ++E+ H + V MTSV GH+++++F W + +P+ LF A K + + + L++E +++W DCDREGENI FE++ + V KP ++ RA+FS IT + +R A +L P++ + +VD R ELDLRIG AFTRFQT Q + L ++LIS+G CQ PTLGF VER+ IQ+F PETF+ +K+ + ++ W R R+F+ + + + A V+ + K K RP AL+TVE+ + AS L IG M +AEKLYTQGFISYPRTET + +L + ++ QT+ +WG + +L+ G PR G HPPI P + L G+ QR+Y++I RH++A S
Sbjct: 38 VNKVLCVAEKNDAAKGIADLMSNSRMRRREGFSKFNKIYEYE-----YHLFGKDVTVVMTSVSGHLLALDFQDHMKKWHSCNPVSLFEAEVQKYCPDDFVNIKRTLEREVRQCQALIIWTDCDREGENIGFEIIHVCKSV--KPNLQVFRARFSEITPRSIRMACEHLVQPDQNISDAVDVRMELDLRIGAAFTRFQTLRLQKMFPSVLSNQLISYGSCQFPTLGFVVERFKAIQAFIPETFYKIKVTHEHEDGLVEFSWKRNRLFNHTACLVLYQICMEDPMATVMEVGSKPKSKWRPVALDTVELEKLASRKLKIGAKETMKVAEKLYTQGFISYPRTETNIFPKDLNLTSLVEQQTHDHQWGGFAQRILERGGPTPRNGNKTDQAHPPIHPTKYT--NSLQGNEQRIYEFIVRHFLACCS 451 HSP 2 Score: 112.464 bits (280), Expect = 1.837e-102 Identity = 68/210 (32.38%), Postives = 105/210 (50.00%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSS----------LADSGKPLTRCGKCNRYMKLIT 2004
A+E F G +I + YL V P+ + +P + P ST + +G+T PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + ++ P +LG+ LV GY + E+ P +R+ +E L LI +G + V+R + + F + ++E F AD+ P+ +C +CN+ M L T
Sbjct: 466 AAERFVAHGLMIIARNYLDVYPYDKWNTKIIPVYEMGARFQP-STVEMVDGETSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTPEQRFLPGELGMGLVEGYDSMGFEMSKPDLRAELESDLKLICEGKKDKFSVLRLQVQKYKQVFVEAAAKATKLDEALSQYFGQAAQPVPQEEIYADTPLPVRKCPQCNKDMVLKT 674
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3b (topoisomerase (DNA) III beta [Source:MGI Symbol;Acc:MGI:1333803]) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 319/426 (74.88%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK----ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVH++ G F + V +KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD++ V + + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V E+SL L+W+R R+FD +A +FL +TK E A V + S KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAG-----QPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 403.675 bits (1036), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 276/397 (69.52%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT G+ +I G+ +MPW + + +LP +G + + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC +G+L+LD ++ P++K+ CN C V+ + V V + +C+ C + L + N + P ++ +GC+FCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-EHFTCMGKTVISPGFTEIMPWQSVPLEESLPT-CQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3b (topoisomerase (DNA) III beta [Source:MGI Symbol;Acc:MGI:1333803]) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 319/426 (74.88%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK----ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVH++ G F + V +KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD++ V + + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V E+SL L+W+R R+FD +A +FL +TK E A V + S KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAG-----QPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 403.675 bits (1036), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 276/397 (69.52%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT G+ +I G+ +MPW + + +LP +G + + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC +G+L+LD ++ P++K+ CN C V+ + V V + +C+ C + L + N + P ++ +GC+FCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-EHFTCMGKTVISPGFTEIMPWQSVPLEESLPT-CQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3b (topoisomerase (DNA) III beta [Source:MGI Symbol;Acc:MGI:1333803]) HSP 1 Score: 327.02 bits (837), Expect = 3.024e-169 Identity = 154/282 (54.61%), Postives = 209/282 (74.11%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQR 2205
DC+ S I+ +G E FT G+ +I G+ +MPW + + +LP +G + + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++
Sbjct: 218 DCKYLQSTISFRIGP-EHFTCMGKTVISPGFTEIMPWQSVPLEESLPT-CQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKK 497 HSP 2 Score: 290.041 bits (741), Expect = 3.024e-169 Identity = 139/215 (64.65%), Postives = 165/215 (76.74%), Query Frame = 3
Query: 675 LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIV-SEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V E+SL L+W+R R+FD +A +FL +TK E A V + S KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 2 LDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 216
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3b (topoisomerase (DNA) III beta [Source:MGI Symbol;Acc:MGI:1333803]) HSP 1 Score: 363.999 bits (933), Expect = 8.998e-119 Identity = 183/294 (62.24%), Postives = 219/294 (74.49%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK----ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSIS 920
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVH++ G F + V +KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD++ V + + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V E+SL L+W+R R+FD +A +FL +TK E A V + S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAG-----QPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATS 289
BLAST of DNA topoisomerase vs. Ensembl Mouse
Match: Top3a (topoisomerase (DNA) III alpha [Source:MGI Symbol;Acc:MGI:1197527]) HSP 1 Score: 295.049 bits (754), Expect = 1.773e-106 Identity = 168/424 (39.62%), Postives = 240/424 (56.60%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
++KV VAEK A IA LS + +G + ++E++ H + V MTSV GH+++ +F ++ W + +P+ LF A K + + L++E H +V+W DCDREGENI FE++ + V KP ++LRA+FS IT +R A NL P++ + +VD RQELDLRIG AFTRFQT Q + + L +LIS+G CQ PTLGF VER+ IQ+F PE F +K+ + +++ W R R+F+ + L + A VV + K K RPQAL+TVE+ + AS L I M IAEKLYTQG+ISYPRTET + +L A ++ QT P WG +++L+ G PR G + HPPI P + L GD +RLY++I RH++A S
Sbjct: 7 GVRKVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKIYEFD-----YHLYGQNVTMIMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETHHCQALVIWTDCDREGENIGFEIIHVCKAV--KPNLRVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHKIKVTHDHKDGTVEFNWKRYRLFNHTACLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPKDLNLVALVEQQTVDPHWGAFAQTILERGGPTPRNGSKSDQAHPPIHPTKYTSG--LQGDDRRLYEFIVRHFLACCS 421 HSP 2 Score: 113.235 bits (282), Expect = 1.773e-106 Identity = 73/236 (30.93%), Postives = 112/236 (47.46%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSG-------------KPLTRCGKCNRYMKLITSTPQRLHCG-----NCRSTCSLP 2058
A E F G ++ + YL V P+ D LP QG ST + +G+T PP LTE++LI+ MEKHGIGTDA+ HI+ I R YV + + ++ P LG+ LV GY + E+ P +R+ +E L LI +G + V+R + + + F+E ++ +L E L + +P+ +C +CN+ M L T + CRS P
Sbjct: 436 AQERFVAHGLIILARNYLDVYPYDHWSDKLLPV-YEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYK---QVFIEAVAKAKKLDEALSQYLGERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFP 667
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|O95985|TOP3B_HUMAN (DNA topoisomerase 3-beta-1 OS=Homo sapiens OX=9606 GN=TOP3B PE=1 SV=1) HSP 1 Score: 552.362 bits (1422), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 322/426 (75.59%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVHE+ G F + VR+KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + +VLWLDCD+EGENICFEVLD++ V +K + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V ++SL L+W+R R+FD+ +A +FL +TK E A+V + S KE K RP ALNTVEMLR ASS LG+GP M AE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAG-----QPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 409.068 bits (1050), Expect = 0.000e+0 Identity = 197/397 (49.62%), Postives = 277/397 (69.77%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT +G+ ++ G+ VMPW + + +LP +G P+ + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ VF KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC SG+L+LD ++ P++K+ CN+C V+ + V V + +C +C + L + N +P D GCVFCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-ELFTCSGKTVLSPGFTEVMPWQSVPLEESLPT-CQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|Q9Z321|TOP3B_MOUSE (DNA topoisomerase 3-beta-1 OS=Mus musculus OX=10090 GN=Top3b PE=1 SV=1) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 270/426 (63.38%), Postives = 319/426 (74.88%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK----ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS ++ KG NG CSVH++ G F + V +KMTSVCGHVM+++F KYN WD VDP ELFS A T K+EANPK+ MV FLQ E G + VVLWLDCD+EGENICFEVLD++ V + + RA+FS+ITD D+ NAM L P+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYGDLDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V E+SL L+W+R R+FD +A +FL +TK E A V + S KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q NHP W V+ LL G RPRKG DAGDHPPITPM+ A + EL GD+ RLY+YI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAG-----QPVHFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 421 HSP 2 Score: 403.675 bits (1036), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 276/397 (69.52%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHAS 2544
DC+ S I+ +G E FT G+ +I G+ +MPW + + +LP +G + + E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + +GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G A++ V+ HT+ +F KF YFV++I+ M+EL E +FS LA +GKPL+RCGKC+R+MK I + P RLHC +C T +LP GT++ YK++RCPLD FELV+WS G++GKS+ LCPYCY +PPF DM++ GCN+C+HP C S + L + C EC +G+L+LD ++ P++K+ CN C V+ + V V + +C+ C + L + N + P ++ +GC+FCD +L++L+HA+
Sbjct: 423 DCKYLQSTISFRIGP-EHFTCMGKTVISPGFTEIMPWQSVPLEESLPT-CQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAA 817
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|O96651|TOP3B_DROME (DNA topoisomerase 3-beta OS=Drosophila melanogaster OX=7227 GN=Top3beta PE=2 SV=2) HSP 1 Score: 489.96 bits (1260), Expect = 0.000e+0 Identity = 239/423 (56.50%), Postives = 299/423 (70.69%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK---ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA S+A LS +CT +G CS HEW G F N G V ++MTSVCGHVMS++FN KYN WD VDPI+LF +T K+E NPK M FL EA G + +VLWLDCD+EGENICFEV+D++++V + RA FSAIT+KD++ AM L +PN+ EA SVDARQELDLRIGCAFTRFQTK+FQ +YGDLDS LIS+GPCQTPTLGFCV+R+D+IQ+FKPE+FW L Q++ + + LEW RGR+F K +A + L K A V S++ KE K +PQALNTVE++R SSGLGIGP M IAE+LYTQG+ISYPRTET Y +FDL A L ++G RS+L PRKG DAGDHPPITPM+ + + D+ R+Y++I RH++ TVS
Sbjct: 1 MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNF-RNEG---SVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL--QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVS 416 HSP 2 Score: 377.867 bits (969), Expect = 0.000e+0 Identity = 192/397 (48.36%), Postives = 251/397 (63.22%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW--LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSD--DMFSGCVFCDKKMCDLMQLEHA 2541
D + V+ + VG E F+ T +ID G+ VM W +D+ P QG + I+ L E QTGPPD+LTESELI+ ME+HGIGTDASIPVHI NI +RNYV I GRK+ PT LGIVLVHGYQ+ID ELVLP MR+ VE L LIA+G AN DV+RH I +F KF YFV+NI M+ LFE +FS LA+SGK +RCGKC RYMK I + P RLHC +C T +LP G V+ Y++ +CPLD F+L+ +S G KG+S+ CPYCY +PPF DM GCN C++ CP S L +++C ECP+G+L+LD + AP +KL CN C V+I + V C C +Q + + + + D + SGC+FC L++ A
Sbjct: 418 DLKYRVTTAKLSVGM-ETFSCTASVLIDAGFTKVMTWSAFGKDEPQPP--FVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALPI-GNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVEKHRA 810
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|Q0J0S6|TOP3B_ORYSJ (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3B PE=2 SV=1) HSP 1 Score: 470.7 bits (1210), Expect = 0.000e+0 Identity = 227/418 (54.31%), Postives = 295/418 (70.57%), Query Frame = 3
Query: 66 VFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCK-ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
V MVAEKPS+A SIA +LS + +T KG VHE++G F +H +K+TSV GHV+S++F Y NW+ DP++LF A ++ E NPK + L +EA G +VLWLDCDREGENIC+EV+D S+ + I RAKFS++T+KD+ +AMNNL P+K EA++VDARQE+DL++G AFTRFQT+YFQGKYG+LDS++IS+GPCQTPTLGFCV+RY +I +FKPE FW LK VI + LEW+R ++FD V +F + + +V ISVKE K RP LNTV +L+ ASS LGIGP M +AE+LYTQGFISYPRTE+T Y SFD R+ L + ++P W VR+LLDTGF +P++G DAGDHPPITPMR A + L D+ RLY YI +H+I TVS
Sbjct: 5 VLMVAEKPSIALSIASALSGGRMSTRKGST---DVHEFDGMFQGSHAF-----FKVTSVIGHVLSVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGENICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDLKVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTYVIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLLKVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDVRTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVS 414 HSP 2 Score: 353.214 bits (905), Expect = 0.000e+0 Identity = 178/414 (43.00%), Postives = 252/414 (60.87%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQ-GTKGKSFVLCPYCYTNPPFEDMQRHSG-----------------CNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHA 2541
PDCR + I G E F G R+ +G+ ++MPWL + +PA +G + I + EG T PPD+L+ESELIS MEK+GIGTDASIPVH+ NI ERNYVQ+ +GR++ PT LG L+ GYQ IDA+L LP +R +E+Q+ LIAKG+A+H VV+H + F K+ YFV+ I M+ LFE FS LADSG+ L++CGKC RYMK I++ P RL+C C LP G+++ YK++ CPLDGFEL+++S G KSF LCP+CY +PPFE + + G C C HP C QS V AC EC +G L+LD +AP+++L CN C ++++ + ++ C C S +++ N K P D + GC+ CD+ + L++++H
Sbjct: 415 PDCRYTRTSIEFTSGG-ETFHCVGNRVTSKGFTSIMPWLAVSENNIPA-YKKGDAVSIHKVDIYEGSTTPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGYQCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFEAQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPLDGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLDDTGKVGKGAGMPCFLCLHPTCKQSMITQGVCACPEC-TGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKKCPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHG 825
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Match: sp|F4ISQ7|TOP3B_ARATH (DNA topoisomerase 3-beta OS=Arabidopsis thaliana OX=3702 GN=At2g32000 PE=2 SV=1) HSP 1 Score: 458.373 bits (1178), Expect = 0.000e+0 Identity = 221/422 (52.37%), Postives = 291/422 (68.96%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEY-VTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
N+ +V MVAEKPS+A SIA LS Q +T +G VHE++G F R + Y++TSV GHV S++F KY NW +DP +LF A IK+E+NPK + L EA G + +VLWLDCDREGENICFEV++S + + K+ RA+FS++T+KD+ AM+NL PN+ EA++VDARQE+DL++G AF+RFQT YFQGKY +LD ++IS+GPCQTPTLGFCV+RY I +FKPE FW L+ + L LEW R R+FD A +F L TA+V+ +S K+ +KGRP LNTV +L+ ASS LG GP M +AE+LYTQGFISYPRTE+T Y SFD L++Q ++P WG V+ LL GF P+ G DAGDHPPITPMR A + + GD+ RLY Y+ +H++ TVS
Sbjct: 3 NLLRVLMVAEKPSIALSIASVLSHGQMSTRRGST---EVHEFDGMF-----RGFKAHYRVTSVIGHVFSVDFPEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVS 416 HSP 2 Score: 369.392 bits (947), Expect = 0.000e+0 Identity = 190/415 (45.78%), Postives = 256/415 (61.69%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWS-QGTKGKSFVLCPYCYTNPPFEDMQRHSG------------------CNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNP--NSDDMFSGCVFCDKKMCDLMQLEHA 2541
P+C+ +K+ + +G E F TG+R+ ++G+ A+MPW D+ LP+ +G+ + + L EG T PPD+LTESELIS MEKHGIGTDASI VHI NI ERNYVQ+ +GRK+ PT LGI L+ GYQ ID +L LP +RS +E+Q+ L+AKG A+H+ VV+H I F KF YFV+ I M+ LFE FS LADSG+ L++CGKC RYMK IT+ P RL CG C LP GTV+ YK++ CPLD FELV++S G +GKSF LCPYCY +PPFE + G C+ C HP C S V AC EC G L+LD + P++KL CN C ++++ + S C C S +++ N K P N + GCV CD+ + L++++H
Sbjct: 417 PNCKYIRTKVELSIGG-ETFHCTGQRVTEKGFTAIMPWSAVDEKKLPS-FLKGERIEVLRVELYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFSPLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFELVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPTCQHSVRNQGVCACPEC-EGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPECDSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHG 828
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A484DAG1 (DNA topoisomerase OS=Perca flavescens OX=8167 GN=EPR50_G00047210 PE=3 SV=1) HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0 Identity = 285/426 (66.90%), Postives = 328/426 (77.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS CT+ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+IE V SK + RAKFS+ITD D+ AMN L PN+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V + S L L+WNR R+FD+ V +F+ LTK A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ QTN+P W V++LL TG RPRKG DAGDHPPITPMR A + EL D RLYDYI RH+IATVS
Sbjct: 1 MKTVLMVAEKPSLAQSIAKILSKGNCTSRKGLNGACSVHEYTGSFQG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKALTEKKEANPKLNMVKFLQVEARGCDYVVLWLDCDKEGENICFEVLDAIEPVMSKGSGRERSVYRAKFSSITDTDIWKAMNGLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVFKGKDSPLTLDWNRVRVFDREVGQMFVNLTKTFLEAQVESVSKKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLEQQTNNPFWAEEVKALLSTGLNRPRKGTDAGDHPPITPMRAASERELGSDGWRLYDYITRHFIATVS 421 HSP 2 Score: 409.838 bits (1052), Expect = 0.000e+0 Identity = 197/400 (49.25%), Postives = 273/400 (68.25%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I +G +EAF+ +G+ +I GY AVMPW + + +PA +G+ + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + GRK++PT LGIVLVHGY + DAELVLP +RSAVE+QL LI+ G AN+ V++HT+ +F KF YFV++I M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK++RCPLD FELV+W+ G +GKS+ LCPYC++NPPF DM++ GCN+C+HP C S L + C EC SG+L+LD + P++++ CN+C V++ V V SCD C + + + N P + +GCVFCD DL++L+HA+ R+
Sbjct: 423 DCKYLQTTIDFNIG-TEAFSCSGKTLISPGYTAVMPWQGIPLEEAMPA-CERGETFTVDEIKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRKLKPTNLGIVLVHGYYKTDAELVLPTIRSAVEKQLTLISLGKANYQQVLQHTLDIFKRKFHYFVDSIPSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELRCPLDDFELVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECDSGVLVLDPISGPKWRMACNKCNVVVHFFEHAHRVQVAQESCDACDASLVAVDFNKIRTPLPAGETQHTGCVFCDLVFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A315VLU1 (DNA topoisomerase OS=Gambusia affinis OX=33528 GN=CCH79_00018594 PE=3 SV=1) HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0 Identity = 282/436 (64.68%), Postives = 334/436 (76.61%), Query Frame = 3
Query: 27 LYVYLNFIFNMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
++ +L + M+ V MVAEKPSLA SIAK LS CT+ KG NG CSVHE+NG F S + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +K + RAKFS+ITD D+ NAMN L PN+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V + + L L+WNR R+FD+ V +F+ L K A+V S+S KE +K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETT Y ++FDL+ L+ Q N+ W V++LL TG RPRKG DAGDHPPITPMR A +GEL D RLY+YI RH+IATVS
Sbjct: 221 VWAHLQKLDKMRTVLMVAEKPSLAQSIAKILSKGSCTSRKGLNGACSVHEYNGNFQS-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLSMVKFLQVEARGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKGIVREQSVFRAKFSSITDTDIWNAMNRLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVFKGKDTPLTLDWNRVRVFDREVGQMFVNLAKISKEAKVESVSKKEKVKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTLYHENFDLKGTLKQQINNTIWAEEVKALLSTGINRPRKGNDAGDHPPITPMRSASEGELGSDGWRLYEYITRHFIATVS 651 HSP 2 Score: 231.106 bits (588), Expect = 0.000e+0 Identity = 123/241 (51.04%), Postives = 168/241 (69.71%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLI--TSTPQRLHCGNCRSTCSLPAGGTVR 2076
DC+ + I +G +EAF+ +G+ +I GY V+PW + + LPA G + L E QT PP +LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + GR+++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA+G AN+ V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + + Q ++ C S S GTV+
Sbjct: 653 DCKYLQTTIDFKIG-TEAFSCSGKTLISPGYTTVIPWQGIPLEEALPA-CECGDTFTVEEIKLVEQQTSPPHYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKANYQQVLQHTLEIFKRKFHYFVDSITSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQVSGSSQSVYA-LCDSVFSCDGLGTVQ 890
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A4U5USK1 (DNA topoisomerase OS=Collichthys lucidus OX=240159 GN=D9C73_013058 PE=3 SV=1) HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0 Identity = 283/427 (66.28%), Postives = 329/427 (77.05%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSK-----PCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS CT+ KG NG C+VHE+NG F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +K C + RAKFS+ITD D+ NAMN L PN+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+W+L+ +V + S L L+WNR R+FD+ V +F+ L K A+V S+S KE K RPQALNTVEMLR ASS LGIGP M IAE+LYTQG+ISYPRTETTHY ++FDL+ LQ QTN+P W V++L TG RPRKG DAGDHPPITPMR A + EL D RLY+YI RH+IATVS
Sbjct: 1 MRSVVMVAEKPSLAQSIAKILSKGSCTSRKGLNGACAVHEYNGSFQG-----QNVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEARGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKGNIREQC-VYRAKFSSITDTDIWNAMNRLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWILQAKVFRGKDSPLTLDWNRVRVFDREVGQMFVNLAKTSREAQVESVSKKEKAKQRPQALNTVEMLRVASSALGIGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGALQQQTNNPIWAEEVKTLFSTGLNRPRKGTDAGDHPPITPMRAASEVELGSDGWRLYEYITRHFIATVS 421 HSP 2 Score: 412.535 bits (1059), Expect = 0.000e+0 Identity = 196/397 (49.37%), Postives = 272/397 (68.51%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHAS 2544
DC+ + I ++G +EAF+ +G+ +I GY AVMPW + + +PA G + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + GRK++PT LGIVLVHGY + DAELVLP +R+AVE+QL+LIA G AN+ V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK++RCPLD FELV+W+ G +GKS+ LCPYC++NPPF DM++ GCN+C+HP C S + C EC SG+L+LD ++ P++++ CN+C V++ V V SCD C + + + N P D +GCVFCD DL++L+HA+
Sbjct: 423 DCKYLQTTIEFIIG-TEAFSCSGKTLISAGYTAVMPWQGIPMEEAMPA-CDLGDTFTVDEIKLVEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRKLKPTNLGIVLVHGYYKTDAELVLPTIRTAVEKQLNLIALGKANYQQVLQHTLDIFKRKFHYFVDSITSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELRCPLDDFELVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSFNSFGIGQCVECDSGVLVLDPTSGPKWRMACNKCNVVVHFFEHAHRVQVAQESCDACDASLVAVDFNKTRTPLPAGDTQHTGCVFCDLVFQDLVELKHAT 817
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A3Q2ZX36 (DNA topoisomerase OS=Kryptolebias marmoratus OX=37003 PE=3 SV=1) HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0 Identity = 283/426 (66.43%), Postives = 328/426 (77.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVS-EKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS CT+ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +K + RAKFS+ITD D+ NAMN L PN+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WVL+ +V + SL L+WNR R+FD+ V +FL L K A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ QT++P W V +LL TG RPRKG DAGDHPPITPMR A +GEL D RLY+YI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSIAKILSKGSCTSRKGLNGACSVHEYTGSFQG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKGVGKDQTVHRAKFSSITDTDIWNAMNCLGKPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVFKGKDNSLTLDWNRVRVFDREVGQMFLNLAKTSREAQVESVSKKEKTKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQTSNPVWAEEVNALLLTGINRPRKGNDAGDHPPITPMRAATEGELGSDGWRLYEYITRHFIATVS 421 HSP 2 Score: 407.142 bits (1045), Expect = 0.000e+0 Identity = 194/402 (48.26%), Postives = 270/402 (67.16%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGI---TQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I ++G +EAF+ G+ +I GY AVMPW + L I +G + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + GRK++PT LGIVLVHGY + DAELVLP +RSAVE+QL+LI+ G AN+ V++H + +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ K++RCPLD FE+V+W+ G +GKS+ LCPYC++NPPF DM++ GCN+C+HP C S L + C EC SG+L+LD + P++++ CN+C V++ V V SCD C + + + N P + +GCVFCD DL++L+HA+ R+
Sbjct: 423 DCKYLQTTIDFVIG-TEAFSCIGKTLISPGYTAVMPW---QGIALEEAIPVCERGDTFTVDEIKLVERQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRKLKPTNLGIVLVHGYYKTDAELVLPTIRSAVEKQLNLISLGKANYQQVLQHALDIFKRKFHYFVDSITSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLNKELRCPLDDFEIVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECDSGVLVLDPLSGPKWRMACNKCNVVVHFFEHAHKVQVAQDSCDACDASLVAVDFNKTSTPLPAGETQHTGCVFCDPVFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. TrEMBL
Match: A0A4W6FPE9 (DNA topoisomerase OS=Lates calcarifer OX=8187 GN=top3b PE=3 SV=1) HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0 Identity = 280/426 (65.73%), Postives = 328/426 (77.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SI+K LS CT+ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EA PK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +K + RAKFS+ITD D+ NAMN L PN+ EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+W+L+ +V + S L L+WNR R+FD+ V +F+ L K A+V S+S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ QTN+P W V++LL TG RPRKG DAGDHPPITPMR A +GEL D RLY+YI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSISKILSKGNCTSRKGLNGACSVHEYTGSFQG-----QTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEATPKLNMVKFLQVEARGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKGSVRERSVYRAKFSSITDTDIWNAMNCLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWILQAKVFKGKDSPLTLDWNRVRVFDREVGQMFVNLAKTSREAQVQSVSKKEKTKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQTNNPIWADEVKALLSTGLNRPRKGTDAGDHPPITPMRAASEGELGSDGWRLYEYITRHFIATVS 421 HSP 2 Score: 411.379 bits (1056), Expect = 0.000e+0 Identity = 197/402 (49.00%), Postives = 271/402 (67.41%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQ---GQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
DC+ + I +G +E F+ +G+ +I GY AVMPW + L + G + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV I GRK++PT LGIVLVHGY +IDAELVLP +RSAVE+QL+LIA G AN+ V++H + +F KF YFV++I+ M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T SLP G ++ YK++RCPLD FELV+W+ G +GKS+ LCPYC++NPPF DM++ GCN+C+HP C S L + C EC SG+L+LD ++ P++++ CN+C V++ V V SCD C + + + N P + +GCVFCD DL++L+HA+ R+
Sbjct: 423 DCKYLQTTIDFSIG-TETFSCSGKTLISPGYTAVMPW---QGIPLEESLPDCECGDTFTVDEIKLVEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTIENGRKLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIALGKANYQQVLQHALDIFKRKFHYFVDSITSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELRCPLDDFELVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGIGQCVECDSGVLVLDPTSGPKWRMACNKCNVVVHFFEHAHRVQVAQESCDACDASLVAVDFNKTRTPLPAGETQHTGCVFCDPIFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3b (DNA topoisomerase III beta [Source:NCBI gene;Acc:103043516]) HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0 Identity = 273/426 (64.08%), Postives = 325/426 (76.29%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS C++ KG NG CSVH++ G F + VR+KMTSVCGHVMS++F KYNNWD VDP EL S A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+I+ V +KP + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+WV++ +V + S + L+W+R R+FD+ + +F+ +TK A V S+S KE K RP ALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q N+ W V++LL G RPRKG DAGDHPPITPMR A + EL +S RLY+YI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSIAKILSKGSCSSRKGLNGACSVHDYTGSFMG-----QSVRFKMTSVCGHVMSLDFTGKYNNWDKVDPAELLSKAPTEKKEANPKLSMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKPYGNERTVYRAKFSSITDTDIWNAMNQLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVIQAKVFKGKDSPITLDWDRVRVFDREIVQMFVNMTKTAREALVESVSKKEKAKQRPLALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNSHWAETVKTLLAEGINRPRKGADAGDHPPITPMRSATESELGSESWRLYEYITRHFIATVS 421 HSP 2 Score: 410.223 bits (1053), Expect = 0.000e+0 Identity = 192/401 (47.88%), Postives = 274/401 (68.33%), Query Frame = 1
Query: 1360 PDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWL-MQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDD--MFSGCVFCDKKMCDLMQLEHASFRN 2553
PDC+ + I+ +G +E F+ TG+ +I G+ VMPW + + LP +G + L E QT PPD+LTE+ELI+ MEKHGIGTDASIPVHI NI +RNYV + GRK++PT LGIVLVHGY +ID ELVLP +RSAVE+QL+LIA G AN V++HT+ +F KF YFV++I+ M+EL E +FS +A +GKP +RCGKC+R+MK I + P RLHC +C T SLP G ++ YK+++CPLD FELV+W+ G++GK++ LCPYC++NPPF DM++ GCN+C+HP CP S L + C EC +G+L+ D ++ P++++ CN+C V++ V V SC+ C + + + + P +D +GCVFCD DL++L+HA+ R+
Sbjct: 422 PDCKYLQTTISFRIG-TEGFSCTGKTLISAGFTEVMPWQGIPLEEALPT-CEKGDAFTVDEVKLLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRKLKPTNLGIVLVHGYYKIDVELVLPTIRSAVEKQLNLIALGKANFHQVLQHTLDIFKRKFHYFVDSIAGMDELMEVSFSPIAATGKPHSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELKCPLDEFELVLWTSGSRGKTYPLCPYCFSNPPFRDMKKGMGCNECTHPTCPHSLNSLGIGQCVECETGVLVFDPNSGPKWRMACNKCNVVVHFFEHAYKVQVAQDSCEACEASLVMVDFHKARTPLADGETQHTGCVFCDTLFQDLVELKHATMRH 820
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 286.96 bits (733), Expect = 8.491e-106 Identity = 165/424 (38.92%), Postives = 241/424 (56.84%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWN-GPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSE-KSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
++KV VAEK A IA+ +S + +G + ++E+ F N V MTSV GH++++ F + + W + +P+ LF A K +++ L+KEA +++W DCDREGENI FEV++ + V + ++ RA+FS IT +R A L P+ + +VD RQELDLRIG AFTRFQT Q + D L ++LIS+G CQ PTLGF VER+ IQ+F PETF+ +K+ SE + ++ W R R+F+ + + E A V+S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + +L ++ QT WG + +L++G PR G + HPPI P + L G+ +RLY++I RH++A S
Sbjct: 31 IKKVLCVAEKNDAAKGIAEIMSSGRARRREGLSVYNKIYEYEYNLFGQN------VTVNMTSVSGHLLALEFKAHFQKWHSCNPVLLFDAEVEKYCPENYVQIKRTLEKEARQCQALIIWTDCDREGENIGFEVINVCKAVKAN-IQVFRARFSEITPNSIRRACETLTEPDINVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPDSLANQLISYGSCQFPTLGFVVERFKAIQAFIPETFFKIKVVHEPSEDECVEFSWKRNRLFNHTACLVLYQMCMEEPLATVLSVTSKPKSKWRPLPLDTVELEKLASRKLKISAKETMKIAEKLYTQGFISYPRTETNMFPTNLNLVPLVEQQTQDNNWGPFAQGILESGGPTPRNGNKSDQAHPPIHPTKYT--NSLQGNEKRLYEFIVRHFLACCS 445 HSP 2 Score: 118.627 bits (296), Expect = 8.491e-106 Identity = 72/221 (32.58%), Postives = 112/221 (50.68%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYM---KLITSTPQRLHC 2028
A E F+ +G +I + YL V P+ + +P P S + EGQT PP LTE++LI+ MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P +R+ +E L L+++G + A V+RH + + A F + ++E + + + P+ +C +CNR M K ST + L C
Sbjct: 460 AEEKFSASGLTIIARNYLDVYPYEKWFNKVIPLYTANTTFQP-SAIEMVEGQTSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKDKASVLRHHVAKYKAVFIESAKKAKKLDEALSNYLGQAQEYTEQEEQELEMPLPVRKCPQCNRDMVLRKRKDSTGKFLSC 679
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 276.944 bits (707), Expect = 7.304e-103 Identity = 150/365 (41.10%), Postives = 216/365 (59.18%), Query Frame = 3
Query: 231 MTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSE-KSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
MTSV GH++++ F + + W + +P+ LF A K +++ L+KEA +++W DCDREGENI FEV++ + V + ++ RA+FS IT +R A L P+ + +VD RQELDLRIG AFTRFQT Q + D L ++LIS+G CQ PTLGF VER+ IQ+F PETF+ +K+ SE + ++ W R R+F+ + + E A V+S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + +L ++ QT WG + +L++G PR G + HPPI P + L G+ +RLY++I RH++A S
Sbjct: 1 MTSVSGHLLALEFKAHFQKWHSCNPVLLFDAEVEKYCPENYVQIKRTLEKEARQCQALIIWTDCDREGENIGFEVINVCKAVKAN-IQVFRARFSEITPNSIRRACETLTEPDINVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPDSLANQLISYGSCQFPTLGFVVERFKAIQAFIPETFFKIKVVHEPSEDECVEFSWKRNRLFNHTACLVLYQMCMEEPLATVLSVTSKPKSKWRPLPLDTVELEKLASRKLKISAKETMKIAEKLYTQGFISYPRTETNMFPTNLNLVPLVEQQTQDNNWGPFAQGILESGGPTPRNGNKSDQAHPPIHPTK--YTNSLQGNEKRLYEFIVRHFLACCS 362 HSP 2 Score: 118.627 bits (296), Expect = 7.304e-103 Identity = 68/208 (32.69%), Postives = 107/208 (51.44%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P P S + EGQT PP LTE++LI+ MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P +R+ +E L L+++G + A V+RH + + A F + ++E + + + P+ +C +CNR M L
Sbjct: 377 AEEKFSASGLTIIARNYLDVYPYEKWFNKVIPLYTANTTFQP-SAIEMVEGQTSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPDLRAELEADLKLVSEGRKDKASVLRHHVAKYKAVFIESAKKAKKLDEALSNYLGQAQEYTEQEEQELEMPLPVRKCPQCNRDMVL 583
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006910.1 (pep scaffold:Pmarinus_7.0:GL489578:1765:6875:1 gene:ENSPMAG00000006247.1 transcript:ENSPMAT00000006910.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 344.354 bits (882), Expect = 1.220e-112 Identity = 164/256 (64.06%), Postives = 197/256 (76.95%), Query Frame = 3
Query: 135 TTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIE-YVTSKPCK--ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQV-IVSEKSLDLEWNRGRIFDKAVANLFLGLTK 887
T+ KG NG CSVHE+ G F + R+KMTSVCGHVM+++F KYNNWD VDP ELFS A T K+EANPK+ M FLQ E G + VVLWLDCDREGENICFEVLD+I Y + + + RAKFSAITD D+ NAMN L PN+ EA++VDARQE+DLR+GCAFTRFQTKYFQGKYGDLD+ L+SFGPCQTPTLGFCVER+D IQSFKPET+WVL+ +V + ++L LEW RGR+FD+ VA FL + +
Sbjct: 4 TSRKGLNGACSVHEYMGTFQG-----QSARFKMTSVCGHVMTLDFIGKYNNWDQVDPAELFSKAPTEKKEANPKLSMGKFLQIEGKGCDYVVLWLDCDREGENICFEVLDAIAPYPVKRGSEQVVFRAKFSAITDTDIHNAMNKLAEPNRYEALAVDARQEVDLRVGCAFTRFQTKYFQGKYGDLDASLVSFGPCQTPTLGFCVERHDRIQSFKPETYWVLQAKVSTAAGQALTLEWERGRMFDREVAQTFLAMVR 254
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Match: top3a (DNA topoisomerase III alpha [Source:ZFIN;Acc:ZDB-GENE-071212-2]) HSP 1 Score: 297.745 bits (761), Expect = 8.950e-106 Identity = 166/423 (39.24%), Postives = 238/423 (56.26%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKY-GDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+++ VAEK A IA +S T +G + ++E+N F+ + V MTSV GH++ + F + + NW P++LF A K N + + L++E +++W DCDREGENI FE++D + V KP ++ RA+FS IT + + NA NL P++ + +VD RQELDLRIG AFTRFQT Q + L +L+S+G CQ PTLGF VER+ IQ+F PE F +K+ + ++ W R R+F+ + L TAEV+ ++ K K RPQ L+TVEM + AS L I M IAEKLYT+GFISYPRTET + +L ++ QT WG + LL+ G PR G + HPPI P + +L+G+ QRLY++I RH++A S
Sbjct: 32 VKRALCVAEKNDAAKGIANIMSRGGFTRREGLSKFNKIYEYNYSFSGQ----QNVNVVMTSVSGHLLGLEFIAGFKNWRGCHPVDLFDAEIEKICLNDLVAIKRTLEREVRQCQALIIWTDCDREGENIGFEIIDVCKAV--KPNLQVFRARFSEITQQSIHNACRNLTLPDENTSKAVDVRQELDLRIGAAFTRFQTLRLQKIFPASLAHQLVSYGSCQFPTLGFVVERFKAIQAFIPEAFHQIKVTHQNEDGLVEFHWKRNRLFNHTACLILYQLCIENPTAEVLEVTCKPKSKWRPQPLDTVEMEKLASRKLKINAKETMKIAEKLYTKGFISYPRTETNVFPKGMNLAELVEMQTQDRNWGAFAQRLLEAG-PNPRNGTKSDQAHPPIHPTKYT--ADLNGNDQRLYEFIVRHFLACCS 445 HSP 2 Score: 105.531 bits (262), Expect = 8.950e-106 Identity = 72/236 (30.51%), Postives = 115/236 (48.73%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFE-------DTFSSLADSG------KPLTRCGKCNRYMKLITSTPQR-----LHCGNCRSTCSLP 2058
A E F G + + YL V P+ + +P T + P S + +G+T PP L+E++LIS MEKHGIGTDA+ HI+ I R YV + ++ P +LG+ LV GY + E+ P +R+ +E L LI +G N+ V++ I + + F+E +S +L E + S+A+ +P+ +C +C M L + + L NCR+ P
Sbjct: 460 AGERFFANGLMITARNYLDVYPYDKWNAKVIPVYTTGVKFQP-SNIEMTDGETSPPQLLSEADLISLMEKHGIGTDATHADHIETIKSRMYVGLTPAQRFLPGELGMGLVEGYDAMGYEMSKPDLRAELEADLKLICEGKKNNTTVLQQQIQKYK---QVFLEAVSKAEKLDEALSCYLGEPQQSIANEETIPRRLEPVRKCPQCKSNMVLKQTKDGKFMLTCLGFPNCRAAVFFP 691
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001990.1 (pep scaffold:Pmarinus_7.0:GL494638:859:4167:1 gene:ENSPMAG00000001806.1 transcript:ENSPMAT00000001990.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 62.3882 bits (150), Expect = 1.601e-11 Identity = 40/111 (36.04%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2212 GCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFS--GCVFCDKKMCDLMQLEH 2538
GCN+C+HP C S L V C EC +G+L+ D +APR+++ CN C VL+ + V V++ + SS +P D GCVFCD L+QL+H
Sbjct: 3 GCNECTHPTCAHSLLALGVGQCVECEAGVLVFDPLSAPRWRMVCNRCSVLVHFFAGALRVKVVAAAAASSSSSAAAAGAKVVRSPLPDGATRHVGCVFCDPVFEPLVQLKH 113
BLAST of DNA topoisomerase vs. Ensembl Yeast
Match: TOP3 (DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination [Source:SGD;Acc:S000004224]) HSP 1 Score: 218.394 bits (555), Expect = 8.452e-80 Identity = 154/472 (32.63%), Postives = 237/472 (50.21%), Query Frame = 3
Query: 63 KVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNG-----PFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTS--KPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDA---RQELDLRIGCAFTRFQTKYFQGKYGDL----------------DSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEK----SLDLEWNRGRIFDKAVANLFLGLTKHET-------TAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTN--------HPEWGTIVRSLL-------DTGFARPRKGL-DAGDHPPITPMRPAG-QGELSGDSQRLYDYIARHYIATVS 1316
KV VAEK S+A ++++ L + T+ +G V ++ PF N E MTSV GH+ I+F+ + W ELF A + N + K+ S +++EA ++ +++W DCDREGE I +E+ + + ++ RA FS + + + NA N P++L+ SV A R E+DLR G FTR T+ + K + DS+++S+G CQ PTLGF V+R++ I++F PE FW IQ++V K + +W+RG +FD+ LT +ET A+VV + K T K RP L TVE+ + + L + + AEKLY +GFISYPRTET + + DL++ ++ Q W + SLL + F PR G D HPPI P+ G + +S +R+Y+Y+ARH++A S
Sbjct: 2 KVLCVAEKNSIAKAVSQILGGGRSTSRD--SGYMYVKNYDFMFSGFPFARNGANCE---VTMTSVAGHLTGIDFSHDSHGWGKCAIQELFDAPLNEIMNNNQKKIASNIKREARNADYLMIWTDCDREGEYIGWEIWQEAKRGNRLIQNDQVYRAVFSHLERQHILNAARN---PSRLDMKSVHAVGTRIEIDLRAGVTFTRLLTETLRNKLRNQATMTKDGAKHRGGNKNDSQVVSYGTCQFPTLGFVVDRFERIRNFVPEEFWY--IQLVVENKDNGGTTTFQWDRGHLFDRLSV-----LTFYETCIETAGNVAQVVDLKSKPTTKYRPLPLTTVELQKNCARYLRLNAKQSLDAAEKLYQKGFISYPRTETDTFPHAMDLKSLVEKQAQLDQLAAGGRTAWASYAASLLQPENTSNNNKFKFPRSGSHDDKAHPPIHPIVSLGPEANVSPVERRVYEYVARHFLACCS 458 HSP 2 Score: 97.4413 bits (241), Expect = 8.452e-80 Identity = 56/160 (35.00%), Postives = 87/160 (54.37%), Query Frame = 1
Query: 1393 MLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYV---QIIAGRKVQPTKLGIVLVHGYQQIDAE--LVLPHMRSAVEEQLDLIAKGDANHADVVRHTI 1857
+L A E F+ +G ++++ +L V PW + + ++ I+ ++ G T PP +TESELI M+ +GIGTDA+I HI I RNYV ++ +QPT LG+ LVHG++ I E P R +E+ L I +G A+ DVV+ +
Sbjct: 469 VLDWAVERFSASGLVVLERNFLDVYPWARWETTKQLPRLEMNALVDIAKAEMKAGTTAPPKPMTESELILLMDTNGIGTDATIAEHIDKIQVRNYVRSEKVGKETYLQPTTLGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVVKDIV 628
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Match: EDO44262 (DNA topoisomerase [Source:UniProtKB/TrEMBL;Acc:A7RW77]) HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0 Identity = 263/424 (62.03%), Postives = 326/424 (76.89%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIE-YVTSKPCK--ILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSE-KSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIAK LS + + KG NG CSVHE++G F R E V++KMTSVCGHVMS++F+ KYNNWDAVDP+ELF+A+T+K+EA K++M FL EA G + +VLWLDCD+EGENICFEV++ + + +P + + RAKFSAIT+KD+ AM L PNK E++SVDARQELDLRIGCAFTRFQTKYFQ KYGDLDS LIS+GPCQTPTLGFCVER+D+IQSFKPE +WV+K +V+ S +S+ L+W+R R+FDK +A +F K + A+V IS + K RPQALNTVEMLR ASSGLG+GP M IAE+LYTQG+ISYPRTETTHY ++FDL+ L+ Q+ WG+ VR LLD +PRKG DAGDHPPITPMR A + EL GD RLYD+I RH++AT+S
Sbjct: 1 MKTVLMVAEKPSLAQSIAKFLSNGRMNSRKGLNGACSVHEYSGSF-----RNEPVKFKMTSVCGHVMSLDFHHKYNNWDAVDPLELFTATTLKKEATEKLQMPKFLSHEAKGVDYLVLWLDCDKEGENICFEVMECVRNSMNKRPGEQTVFRAKFSAITEKDIAGAMQKLGQPNKNESLSVDARQELDLRIGCAFTRFQTKYFQNKYGDLDSALISYGPCQTPTLGFCVERHDQIQSFKPEAYWVIKPKVVHSSGQSVVLDWDRVRVFDKEIALVFQKTIKESSNAQVTCISKTQKTKQRPQALNTVEMLRLASSGLGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGALKQQSGSAMWGSHVRELLDGAMTKPRKGHDAGDHPPITPMRCASESELGGDPWRLYDFITRHFLATLS 419 HSP 2 Score: 426.787 bits (1096), Expect = 0.000e+0 Identity = 210/403 (52.11%), Postives = 277/403 (68.73%), Query Frame = 1
Query: 1354 LMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPW-LMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRN 2553
L PDC+ + + +G EAF+ TG+ + D G+ +VM W + DD +P +G + ++ L E QT PPDFLTESELI+ MEKHGIGTDASI VHI NI ERNYVQI +GRK+ PT LGIVLVHGYQ+ID ELVLP MRSAVEEQL+LIA G A+ V+ H I +F+ KF YFV+ +S M+ELFE +F+ L++SGKPL+RCGKCNRYMK I + P RLHC C T SLP G+++ YK++RCPLD FELV+WS G KGKS +CPYCY++PPF M++ GCN+C+HP CP S+ L V C EC G+L LD ++AP+++L CN C V+I M +++ CD C S L + N ++ PN+ GC FCD + L++L+HA+ R+
Sbjct: 418 LSPDCKYLQTTVKFKIG-KEAFSFTGKAVTDPGFTSVMTWQAISDDERMPP-FNKGDICQVAEVKLDERQTLPPDFLTESELITLMEKHGIGTDASISVHINNICERNYVQIQSGRKLAPTSLGIVLVHGYQKIDPELVLPTMRSAVEEQLNLIAHGKADFNAVLHHAIGIFTQKFTYFVKTVSSMDELFEVSFTPLSESGKPLSRCGKCNRYMKYIAAKPSRLHCATCDETYSLPQNGSIKLYKELRCPLDDFELVLWSTGAKGKSTPVCPYCYSHPPFPTMRKGMGCNQCTHPTCPHSEEALGVADCLECEKGMLNLDPTSAPKWRLACNMCNVVIHMCEGAFRISLSEDECD-CGSSKLDVDFNKANSPLPNNLTQHRGCPFCDPILVPLVRLQHAASRH 817
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Match: EDO40018 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S883]) HSP 1 Score: 271.937 bits (694), Expect = 3.193e-98 Identity = 161/423 (38.06%), Postives = 235/423 (55.56%), Query Frame = 3
Query: 63 KVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC---KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKY-GDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+V VAEK A +++ +S QCT +G + ++E+N + + R+ MTSV GH+MS+ F Y W + P+ LF A +K + + L+ E GS +++W DCDREGENI E++D V +K C K+ RA+FS IT + ++ A NL P++L++ +VD R ELDLRIG AFTRFQT Q + G L +LIS+G CQ PTLGF VERY ++Q F PE F+ +K+ + +D W RGR+F + + + TA+V+S S + K RP L+TVE+ + AS L I M IAEKLYTQG S TETT ++ ++S ++ E+ T S + PR+G + HPPI P + L G+ +RLY++I RH++A S
Sbjct: 2 RVLNVAEKNDAAKELSRIMSRGQCTRREGQSRFNKIYEFNFNILNANARM-----VMTSVSGHLMSLEFAIAYRKWSSCSPLSLFDAPVVKNVGEDYLPIKKTLECEVRGSQALIIWTDCDREGENIGHEIID----VCTKVCPRIKVYRARFSEITPQSVQRACANLVAPDRLQSEAVDVRMELDLRIGAAFTRFQTLRLQKVFPGFLSDQLISYGSCQFPTLGFVVERYKQVQQFVPEQFFKIKVSHETPDGQVDFVWKRGRLFHRLPCLILYQMCLESPTAKVLSTSSRPKSKWRPLPLDTVELEKLASRKLHINAKETMKIAEKLYTQGRYSVTGTETTTFN-------AVESMISYIEFVTGFASDVLANGPNPRQGSKTDNAHPPIHPTQYTNN--LQGNDKRLYEFIVRHFLACCS 406 HSP 2 Score: 106.686 bits (265), Expect = 3.193e-98 Identity = 67/214 (31.31%), Postives = 102/214 (47.66%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLAD---------SGKPLTR-CGKCNRYMKLITSTPQ 2016
A E FT TG + + YL V P+ +P T + P S + +G+T PP LTE++LI+ MEKHGIGTDA+ HI+ I R+YV + P +LG+ LV GY + E P +RS +E L I G + A V+R I + F + +++ + F AD + P+ R C +C ++ + T +
Sbjct: 421 AGEKFTATGLMITARNYLDVYPYDRWSTKVIPVYETNEEFQPTSI-EMVDGETTPPPLLTEADLIALMEKHGIGTDATHADHIETIKSRSYVGVQGDGTFLPGQLGMGLVEGYDLMHFEFSKPKLRSGLEADLKRICDGTKSGAVVLREQIAKYKENFIQAAQEAIKLDQALSNYFGDPADFIEQELDPLAADPIVRPCPRCEQHQMTLRKTKE 633
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Match: EDO28677 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5T3]) HSP 1 Score: 62.3882 bits (150), Expect = 8.418e-11 Identity = 62/250 (24.80%), Postives = 103/250 (41.20%), Query Frame = 3
Query: 75 VAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSI----NFNSKYNNWDAVD-PIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVD-------ARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLK 788
+AEKPS+A IA L N T G E Y +T GH+ ++ ++ + +WD + P+ T K + ++ + +V+ D +EGE I V++ Y K+ R S++T++ ++ NL+ +A++ D +R D +G TR T F G +++S G QTPTL V RY EI+ F P+ +W L+
Sbjct: 5 IAEKPSVAREIANILGAN---------------------TKRDGYFEGNGYAVTYTFGHLCTLLEPKDYKPHWKSWDLNNLPMLPERFDTKVTNDTGIQKQFNIIKSLFEKAEVVINCGDAGQEGELIQRWVINQAGYT----GKVQRLWISSLTEEAIKEGFENLE-----DAVNYDNLYYAGYSRAIGDWLLGLNATRLYTVKFGGY-----KQVLSIGRVQTPTLAMLVNRYKEIEHFTPKPYWELQ 219
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3b (DNA topoisomerase III beta [Source:NCBI gene;Acc:101167966]) HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0 Identity = 274/426 (64.32%), Postives = 323/426 (75.82%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFS-ASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPC----KILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKS-LDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
M+ V MVAEKPSLA SIA+ LS CT+ KG NG CSVHE+ G F + VR+KMTSVCGHVMS++F KYNNWD VDP ELFS A T K+EANPK+ MV FLQ EA G + VVLWLDCD+EGENICFEVLD+++ V +K + RAKFS+ITD D+ NAMN L PNK EA+SVDARQELDLRIGCAFTRFQTKYFQGKYG+LDS LISFGPCQTPTLGFCVER+D+IQSFKPET+W+L+ +V + L L+W+R R+FD+ V +F+ L K A+V ++S KE K RPQALNTVEMLR ASS LG+GP M IAE+LYTQG+ISYPRTETT Y ++FDL+ L+ Q N P W V++LL G RPRKG D GDHPPITPMR A +GEL D RLY+YI RH+IATVS
Sbjct: 1 MRTVLMVAEKPSLAQSIARILSKGSCTSRKGLNGACSVHEYTGTFQG-----QSVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLSMVKFLQVEARGCDFVVLWLDCDKEGENICFEVLDAVQPVMNKGGVREQTVFRAKFSSITDTDIWNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWILQAKVFKGKDGPLTLDWSRVRVFDREVGQMFVNLAKMSKEAKVEAVSQKEKTKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTQYPENFDLKGTLRQQANSPMWAEEVKALLSAGLNRPRKGNDVGDHPPITPMRAASEGELGSDGWRLYEYITRHFIATVS 421 HSP 2 Score: 410.994 bits (1055), Expect = 0.000e+0 Identity = 198/410 (48.29%), Postives = 271/410 (66.10%), Query Frame = 1
Query: 1363 DCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPS---------LREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDN--PNSDDMFSGCVFCDKKMCDLMQLEHASFRNNS 2559
DCR S I +G +E F+ G+ I GY A+MPW G+ + LP P L E QTGPPD+LTE+ELI+ MEKHGIGTDASIPVHI N+ +RNYV + GR+++PT LG+VLVHGY +IDAELVLP +RSAVE+QL LIA G AN V++HT+ +F KF YFV++IS M+EL E +FS +A +GKPL+RCGKC+R+MK I + P RLHC +C T ++P G ++ YK++RCPLD FELV+W+ G +GKS+ LCPYC++NPPF DM++ GCN+C+HP C S + L + C EC SG+L+LD ++ P++++ CN C VL+ + V V SC++C + + + N P D +GCVFCD D ++L+HAS R +
Sbjct: 423 DCRYLQSTIEFSIG-TERFSCGGKTPISPGYTALMPW---------QGVPLEEALPTCQPGDAFTVDELRLVERQTGPPDYLTEAELITLMEKHGIGTDASIPVHINNVCQRNYVTVETGRRLKPTNLGVVLVHGYYKIDAELVLPTIRSAVEKQLSLIALGKANFQQVLQHTLDIFKRKFHYFVDSISSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYNVPQNGAIKLYKELRCPLDDFELVLWTSGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLSALGIGQCVECESGVLVLDPTSGPKWRMACNRCNVLVHLFEHAHKVQVSQDSCEVCDASLVMVDFNKARTPLPEGDTQHTGCVFCDPLFQDAVELKHASMRRTA 822
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 279.641 bits (714), Expect = 2.195e-102 Identity = 160/425 (37.65%), Postives = 240/425 (56.47%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLD-TGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+++V VAEK A I++ +S + +G + ++E+ + V MTSV GH++++ F + + W + +P+ LF A K + + + L+KE +V+W DCDREGENI FEV+D + V KP ++ RAKFS IT +R A L P+ + +VD RQELDLRIG +FTRFQT Q + + L ++L+S+G CQ PTLGF VER+ I++F PETF+ +K+ + + +++ W R R+F+ + + + A V S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + L ++ QT+ WGT + +LD G PR+G + HPPI P + L G+ R+Y++I RH++A VS
Sbjct: 50 QIKRVLCVAEKNDAAKGISEIMSSGRARRREGMSKFNKIYEYEYFLFG-----QNVTVTMTSVSGHLLALEFKAPFQKWHSCNPVMLFDAEVEKYCPDNMIPIKRTLEKEVRQCQALVIWTDCDREGENIGFEVIDVCKAV--KPNLQVFRAKFSEITPASIRRACETLIEPDANISDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLANQLVSYGSCQFPTLGFVVERFKAIKAFIPETFYKIKVLHTLEDDTVEFSWKRNRLFNHTCCLVLYQICMEDPIATVTSVTSKPKSKWRPLPLDTVELEKLASRKLRINAKETMKIAEKLYTQGFISYPRTETNIFPANLVLAPLVEQQTHSSTWGTFAQRVLDQPGGPNPRQGKKSDQAHPPIHPTK--YTSSLQGNEARVYEFIVRHFLACVS 465 HSP 2 Score: 114.39 bits (285), Expect = 2.195e-102 Identity = 68/208 (32.69%), Postives = 105/208 (50.48%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P Q P S + +GQT PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P++RS +E L L+++G + V++H I + F V ++E + + + P+ +C C R M L
Sbjct: 480 AQEKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQP-SAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAALEIPEAQQQDLEMPLPVRKCPNCGRDMVL 686
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 279.641 bits (714), Expect = 2.232e-102 Identity = 160/425 (37.65%), Postives = 240/425 (56.47%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLD-TGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+++V VAEK A I++ +S + +G + ++E+ + V MTSV GH++++ F + + W + +P+ LF A K + + + L+KE +V+W DCDREGENI FEV+D + V KP ++ RAKFS IT +R A L P+ + +VD RQELDLRIG +FTRFQT Q + + L ++L+S+G CQ PTLGF VER+ I++F PETF+ +K+ + + +++ W R R+F+ + + + A V S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + L ++ QT+ WGT + +LD G PR+G + HPPI P + L G+ R+Y++I RH++A VS
Sbjct: 48 QIKRVLCVAEKNDAAKGISEIMSSGRARRREGMSKFNKIYEYEYFLFG-----QNVTVTMTSVSGHLLALEFKAPFQKWHSCNPVMLFDAEVEKYCPDNMIPIKRTLEKEVRQCQALVIWTDCDREGENIGFEVIDVCKAV--KPNLQVFRAKFSEITPASIRRACETLIEPDANISDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLANQLVSYGSCQFPTLGFVVERFKAIKAFIPETFYKIKVLHTLEDDTVEFSWKRNRLFNHTCCLVLYQICMEDPIATVTSVTSKPKSKWRPLPLDTVELEKLASRKLRINAKETMKIAEKLYTQGFISYPRTETNIFPANLVLAPLVEQQTHSSTWGTFAQRVLDQPGGPNPRQGKKSDQAHPPIHPTK--YTSSLQGNEARVYEFIVRHFLACVS 463 HSP 2 Score: 114.39 bits (285), Expect = 2.232e-102 Identity = 68/208 (32.69%), Postives = 105/208 (50.48%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P Q P S + +GQT PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P++RS +E L L+++G + V++H I + F V ++E + + + P+ +C C R M L
Sbjct: 478 AQEKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQP-SAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAALEIPEAQQQDLEMPLPVRKCPNCGRDMVL 684
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 279.641 bits (714), Expect = 2.681e-102 Identity = 160/425 (37.65%), Postives = 240/425 (56.47%), Query Frame = 3
Query: 54 NMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLD-TGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+++V VAEK A I++ +S + +G + ++E+ + V MTSV GH++++ F + + W + +P+ LF A K + + + L+KE +V+W DCDREGENI FEV+D + V KP ++ RAKFS IT +R A L P+ + +VD RQELDLRIG +FTRFQT Q + + L ++L+S+G CQ PTLGF VER+ I++F PETF+ +K+ + + +++ W R R+F+ + + + A V S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + L ++ QT+ WGT + +LD G PR+G + HPPI P + L G+ R+Y++I RH++A VS
Sbjct: 7 QIKRVLCVAEKNDAAKGISEIMSSGRARRREGMSKFNKIYEYEYFLFG-----QNVTVTMTSVSGHLLALEFKAPFQKWHSCNPVMLFDAEVEKYCPDNMIPIKRTLEKEVRQCQALVIWTDCDREGENIGFEVIDVCKAV--KPNLQVFRAKFSEITPASIRRACETLIEPDANISDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLANQLVSYGSCQFPTLGFVVERFKAIKAFIPETFYKIKVLHTLEDDTVEFSWKRNRLFNHTCCLVLYQICMEDPIATVTSVTSKPKSKWRPLPLDTVELEKLASRKLRINAKETMKIAEKLYTQGFISYPRTETNIFPANLVLAPLVEQQTHSSTWGTFAQRVLDQPGGPNPRQGKKSDQAHPPIHPTK--YTSSLQGNEARVYEFIVRHFLACVS 422 HSP 2 Score: 114.39 bits (285), Expect = 2.681e-102 Identity = 68/208 (32.69%), Postives = 105/208 (50.48%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P Q P S + +GQT PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P++RS +E L L+++G + V++H I + F V ++E + + + P+ +C C R M L
Sbjct: 437 AQEKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQP-SAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAALEIPEAQQQDLEMPLPVRKCPNCGRDMVL 643
BLAST of DNA topoisomerase vs. Ensembl Medaka
Match: top3a (DNA topoisomerase III alpha [Source:NCBI gene;Acc:101168371]) HSP 1 Score: 271.937 bits (694), Expect = 5.078e-100 Identity = 149/366 (40.71%), Postives = 216/366 (59.02%), Query Frame = 3
Query: 231 MTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGD-LDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLD-TGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
MTSV GH++++ F + + W + +P+ LF A K + + + L+KE +V+W DCDREGENI FEV+D + V KP ++ RAKFS IT +R A L P+ + +VD RQELDLRIG +FTRFQT Q + + L ++L+S+G CQ PTLGF VER+ I++F PETF+ +K+ + + +++ W R R+F+ + + + A V S++ K K RP L+TVE+ + AS L I M IAEKLYTQGFISYPRTET + + L ++ QT+ WGT + +LD G PR+G + HPPI P + L G+ R+Y++I RH++A VS
Sbjct: 1 MTSVSGHLLALEFKAPFQKWHSCNPVMLFDAEVEKYCPDNMIPIKRTLEKEVRQCQALVIWTDCDREGENIGFEVIDVCKAV--KPNLQVFRAKFSEITPASIRRACETLIEPDANISDAVDVRQELDLRIGASFTRFQTLRLQKIFPESLANQLVSYGSCQFPTLGFVVERFKAIKAFIPETFYKIKVLHTLEDDTVEFSWKRNRLFNHTCCLVLYQICMEDPIATVTSVTSKPKSKWRPLPLDTVELEKLASRKLRINAKETMKIAEKLYTQGFISYPRTETNIFPANLVLAPLVEQQTHSSTWGTFAQRVLDQPGGPNPRQGKKSDQAHPPIHPTK--YTSSLQGNEARVYEFIVRHFLACVS 362 HSP 2 Score: 114.39 bits (285), Expect = 5.078e-100 Identity = 68/208 (32.69%), Postives = 105/208 (50.48%), Query Frame = 1
Query: 1405 ASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGK----------PLTRCGKCNRYMKL 1998
A E F+ +G +I + YL V P+ + +P Q P S + +GQT PP LTE++LIS MEKHGIGTDA+ HI+ I R YV + A ++ P +LG+ LV GY + E+ P++RS +E L L+++G + V++H I + F V ++E + + + P+ +C C R M L
Sbjct: 377 AQEKFSTSGLMIIARNYLDVYPYDKWNAKVIPVYEEDSQFQP-SAIEMVDGQTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTADQRFLPGELGMGLVEGYNSMGYEMSKPNLRSELEADLKLVSEGRKDKRGVLQHHIQKYKTVFIESVRKAKKLDEALSAYLGAALEIPEAQQQDLEMPLPVRKCPNCGRDMVL 583
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000002574.1 (SMESG000002574.1) HSP 1 Score: 880.167 bits (2273), Expect = 0.000e+0 Identity = 418/419 (99.76%), Postives = 419/419 (100.00%), Query Frame = 3
Query: 57 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATV 1313
MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIAT+
Sbjct: 1 MQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATL 419 HSP 2 Score: 843.573 bits (2178), Expect = 0.000e+0 Identity = 402/409 (98.29%), Postives = 403/409 (98.53%), Query Frame = 1
Query: 1342 YPFQLMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN 2568
Y LMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLP GITQGQML ISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENI+WMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPEC QSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN
Sbjct: 415 YIATLMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPVGITQGQMLSISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENIAWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECHQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN 823
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000002574.1 (SMESG000002574.1) HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0 Identity = 413/414 (99.76%), Postives = 414/414 (100.00%), Query Frame = 3
Query: 72 MVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATV 1313
MVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIAT+
Sbjct: 1 MVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFLGLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATL 414 HSP 2 Score: 843.958 bits (2179), Expect = 0.000e+0 Identity = 402/409 (98.29%), Postives = 403/409 (98.53%), Query Frame = 1
Query: 1342 YPFQLMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECPQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN 2568
Y LMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLP GITQGQML ISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENI+WMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPEC QSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN
Sbjct: 410 YIATLMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPVGITQGQMLSISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENIAWMNELFEDTFSSLADSGKPLTRCGKCNRYMKLITSTPQRLHCGNCRSTCSLPAGGTVRQYKQVRCPLDGFELVVWSQGTKGKSFVLCPYCYTNPPFEDMQRHSGCNKCSHPECHQSQAKLVVTACHECPSGLLLLDDSNAPRFKLQCNECPVLIMMQPSIVVVNVLSTSCDLCSSQHLQLKLNTKDNPNSDDMFSGCVFCDKKMCDLMQLEHASFRNNSVNN 818
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081337.1 (SMESG000081337.1) HSP 1 Score: 257.684 bits (657), Expect = 9.498e-90 Identity = 160/427 (37.47%), Postives = 238/427 (55.74%), Query Frame = 3
Query: 66 VFMVAEKPSLAHSIAKSLSFNQCTTSKGFNGVCSVHEWNGPFTSNHGRVEQVRYKMTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKP-CKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYGDL-DSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLE---WNRGRIFDKAVANLFLGLTKHETT----AEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGD-HPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
+ VAEK A +I+ LS +G + ++E+ +Q MTSV GH+M+ F + +NW VDP ELF K + + + L+ N++++W DCDREGENI E++D+ + KP + RAKFS IT + + A+NNL N + +VD RQE+DL++G AFTR QT Y Q K+ L S++IS+G CQ PT+GF V+RY E++SF+ E FW + + EK L++ W+R R+FD+ NL L L ++ T A V+ + K K RP A++T+E+ + AS L I P M++AEKLY++GFISYPRTET + +L +++Q WG LL G P G + HPPI P++ G G L+ D R+++ I RH++A +S
Sbjct: 3 ILNVAEKNDAAKAISGFLSNGTARKKEGKSVYNKIYEFKYNLNG-----QQTDMIMTSVSGHMMNFEFPIRMSNWKGVDPEELFDCPVNKVCTDNMKNVKASLENLIKSCNMLIIWTDCDREGENIGMEIVDTCLAI--KPNITVKRAKFSEITFQSVTRALNNLVDINLRLSQAVDTRQEIDLKLGSAFTRLQTLYLQKKFPRLLSSQIISYGSCQFPTMGFVVDRYKEVESFESEPFWKINLN---HEKKLEIAEFIWDRIRLFDE---NLVLALLENITDGGCLACVIDVKNKPKSKWRPFAMDTIELEKLASRKLKINPKTTMNLAEKLYSKGFISYPRTETNIFPKEMNLSKLVENQVPDNRWGGFATRLLSEGI-HPHNGKKSDQAHPPIHPLK-QGIG-LNEDEARIFELITRHFLACLS 413 HSP 2 Score: 93.9745 bits (232), Expect = 9.498e-90 Identity = 57/171 (33.33%), Postives = 91/171 (53.22%), Query Frame = 1
Query: 1411 EAFTLTGRRMIDQGYLAVMPWLMQDDVTLPA-GITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAG--RKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNE 1914
E F+LTG ++++ YL V P+ D +P GI G++ ++ S+ + +T P LTE++L+ M+KHGIGTDA+ HI+ + R+YV + P KLG+ LV GY + L P +RS E L+LI G +V+ H + + +K I+ + E
Sbjct: 451 EGFSLTGLSVLERNYLDVYPYDKWVDKEVPVYGI--GEIFEPTSVSISQSETSAPSLLTEADLLILMDKHGIGTDATHAEHIEKVKHRDYVGNTGPGMNFLIPGKLGMGLVEGYSLVGQLLSRPDLRSQFEADLELICNGTKQPLEVLNHYLNAYKEIYKNTNTKINKLKE 619
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081353.1 (SMESG000081353.1) HSP 1 Score: 236.498 bits (602), Expect = 9.656e-85 Identity = 141/364 (38.74%), Postives = 204/364 (56.04%), Query Frame = 3
Query: 231 MTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYG-DLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFL-GLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
MTSV GH M F NW V+PI LF + K + + LQK A +++W DCDREGENI E++D V SK + RA+FS IT + + A+NNL N + +VD RQELDL+ G AF+R QT Q K + SK+IS+G CQ PTLGF V+RY E+ F + FW + + V+E + W R D+ + L +T++ + A V+++ K+++K RP AL+T+ M + AS+ L P V M +AE LY +GFISYPRTET +S +L + +QT+ WG +++L G + HPPI P++ G G L+ D L++ I RH++A +S
Sbjct: 3 MTSVSGHFMDFVFPEHLMNWKTVNPIALFDSPVDKICLKNMVGVKESLQKIATRCQRLIIWTDCDREGENIGMEIVDVCLAVNSK-IDVKRARFSEITAESVTRAINNLVNMNLNVSKAVDVRQELDLKTGAAFSRLQTLSLQNKIPVNFTSKVISYGSCQFPTLGFVVDRYKEVTEFVSKPFWKIILTQKVNESKFEFNWCTERSLDENIIKKSLEKITQNGSLACVINVVNKKSIKKRPIALDTIRMEKLASNKLLFTPKVTMKLAEILYCKGFISYPRTETNMFSKEVNLHKFVAAQTSDIRWGDFAKNILLDGITPMCGNKNDNAHPPIIPLK-LGIG-LAFDENHLFELITRHFLACLS 363 HSP 2 Score: 98.5969 bits (244), Expect = 9.656e-85 Identity = 58/172 (33.72%), Postives = 89/172 (51.74%), Query Frame = 1
Query: 1402 GASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNEL 1917
G E F+LTG + ++ YL V P+ D +P G + S ++ G+T PP FLT+ +LIS M+K+GIGTDA+ HI I R Y + PTKLGI L+ Y + + P +RS E L I G + +V+RH + + + F ++NI + ++
Sbjct: 398 GLGEEFSLTGTIVREKNYLDVYPYEKLLDTFIP-NYEIGDIFEPSCVGIKMGKTCPPSFLTDVDLISLMDKYGIGTDATHAEHIYKIKVREYTCLKYPNFTVPTKLGIGLIDAYNYLGINIAQPMLRSKFEADLKEICNGTKDPNEVLRHYLDEYKSIFLESIKNIDKLQDI 568
BLAST of DNA topoisomerase vs. Planmine SMEST
Match: SMESG000081353.1 (SMESG000081353.1) HSP 1 Score: 236.113 bits (601), Expect = 1.229e-84 Identity = 141/364 (38.74%), Postives = 204/364 (56.04%), Query Frame = 3
Query: 231 MTSVCGHVMSINFNSKYNNWDAVDPIELFSASTIKQEANPKMKMVSFLQKEAHGSNIVVLWLDCDREGENICFEVLDSIEYVTSKPCKILRAKFSAITDKDLRNAMNNLQYPNKLEAMSVDARQELDLRIGCAFTRFQTKYFQGKYG-DLDSKLISFGPCQTPTLGFCVERYDEIQSFKPETFWVLKIQVIVSEKSLDLEWNRGRIFDKAVANLFL-GLTKHETTAEVVSISVKETLKGRPQALNTVEMLRTASSGLGIGPSVCMSIAEKLYTQGFISYPRTETTHYSDSFDLRAPLQSQTNHPEWGTIVRSLLDTGFARPRKGLDAGDHPPITPMRPAGQGELSGDSQRLYDYIARHYIATVS 1316
MTSV GH M F NW V+PI LF + K + + LQK A +++W DCDREGENI E++D V SK + RA+FS IT + + A+NNL N + +VD RQELDL+ G AF+R QT Q K + SK+IS+G CQ PTLGF V+RY E+ F + FW + + V+E + W R D+ + L +T++ + A V+++ K+++K RP AL+T+ M + AS+ L P V M +AE LY +GFISYPRTET +S +L + +QT+ WG +++L G + HPPI P++ G G L+ D L++ I RH++A +S
Sbjct: 1 MTSVSGHFMDFVFPEHLMNWKTVNPIALFDSPVDKICLKNMVGVKESLQKIATRCQRLIIWTDCDREGENIGMEIVDVCLAVNSK-IDVKRARFSEITAESVTRAINNLVNMNLNVSKAVDVRQELDLKTGAAFSRLQTLSLQNKIPVNFTSKVISYGSCQFPTLGFVVDRYKEVTEFVSKPFWKIILTQKVNESKFEFNWCTERSLDENIIKKSLEKITQNGSLACVINVVNKKSIKKRPIALDTIRMEKLASNKLLFTPKVTMKLAEILYCKGFISYPRTETNMFSKEVNLHKFVAAQTSDIRWGDFAKNILLDGITPMCGNKNDNAHPPIIPLK-LGIG-LAFDENHLFELITRHFLACLS 361 HSP 2 Score: 98.5969 bits (244), Expect = 1.229e-84 Identity = 58/172 (33.72%), Postives = 89/172 (51.74%), Query Frame = 1
Query: 1402 GASEAFTLTGRRMIDQGYLAVMPWLMQDDVTLPAGITQGQMLPISTPSLREGQTGPPDFLTESELISKMEKHGIGTDASIPVHIQNIVERNYVQIIAGRKVQPTKLGIVLVHGYQQIDAELVLPHMRSAVEEQLDLIAKGDANHADVVRHTIMVFSAKFKYFVENISWMNEL 1917
G E F+LTG + ++ YL V P+ D +P G + S ++ G+T PP FLT+ +LIS M+K+GIGTDA+ HI I R Y + PTKLGI L+ Y + + P +RS E L I G + +V+RH + + + F ++NI + ++
Sbjct: 396 GLGEEFSLTGTIVREKNYLDVYPYEKLLDTFIP-NYEIGDIFEPSCVGIKMGKTCPPSFLTDVDLISLMDKYGIGTDATHAEHIYKIKVREYTCLKYPNFTVPTKLGIGLIDAYNYLGINIAQPMLRSKFEADLKEICNGTKDPNEVLRHYLDEYKSIFLESIKNIDKLQDI 566 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of DNA topoisomerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA topoisomerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA topoisomerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of DNA topoisomerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA topoisomerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033081 ID=SMED30033081|Name=DNA topoisomerase|organism=Schmidtea mediterranea sexual|type=transcript|length=2706bpback to top protein sequence of SMED30033081-orf-1 >SMED30033081-orf-1 ID=SMED30033081-orf-1|Name=SMED30033081-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp MALHILEWRIRVAVRAQDEALALGALGPDNQFEPVQGTSDLFILSDSASRback to top protein sequence of SMED30033081-orf-2 >SMED30033081-orf-2 ID=SMED30033081-orf-2|Name=SMED30033081-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=435bp MVIDIDYPFQLMPDCRMEVSKITMLVGASEAFTLTGRRMIDQGYLAVMPWback to top protein sequence of SMED30033081-orf-3 >SMED30033081-orf-3 ID=SMED30033081-orf-3|Name=SMED30033081-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=444bp LIIIFKYFLYVYLNFIFNMQKVFMVAEKPSLAHSIAKSLSFNQCTTSKGFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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