RNA polymerase II-associated factor 1

Overview
NameRNA polymerase II-associated factor 1
Smed IDSMED30033043
Length (bp)1410
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNA polymerase II-associated factor 1 (SMED30033043) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA polymerase II-associated factor 1 (SMED30033043) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA polymerase II-associated factor 1 (SMED30033043) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA polymerase II-associated factor 1 (SMED30033043) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033043

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033043SMESG000006276.1 SmedASXL_008469SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30033043SMESG000006276.1 SmedASXL_008469SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Human
Match: PAF1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:25459])

HSP 1 Score: 341.658 bits (875), Expect = 9.034e-112
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Human
Match: PAF1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:25459])

HSP 1 Score: 339.347 bits (869), Expect = 1.740e-111
Identity = 185/373 (49.60%), Postives = 242/373 (64.88%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
            MAPTIQ            +R +     ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:    1 MAPTIQTQ---------AQREDGHRSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 361
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Human
Match: PAF1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:25459])

HSP 1 Score: 221.861 bits (564), Expect = 1.439e-68
Identity = 118/232 (50.86%), Postives = 153/232 (65.95%), Query Frame = 3 
Query:  333 LHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVR 1016
            L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELET ++
Sbjct:   47 LDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETSIQ 278
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Celegans
Match: pafo-1 (RNA polymerase II-associated factor 1 homolog [Source:UniProtKB/Swiss-Prot;Acc:P90783])

HSP 1 Score: 169.859 bits (429), Expect = 1.308e-47
Identity = 115/354 (32.49%), Postives = 197/354 (55.65%), Query Frame = 3 
Query:  120 LICKVKYTNTLPDIPFDPKFLQYSFGN-ERFIKYNPTSLERNYKHDLLTEHDVGVEVDLID-------PLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLG---RNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTY-YNELETRVRLNKRRKIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             + K ++TNT+PD+PFD KF+   F    RF+++ P ++ R+YKH ++ + D+G+ VDLID       P+  +I+ +D +  +D+       ++ L ++RS+ H+K V WMRKTEYIS E +      D  E+++GY  K   + +D+YRD++SQI+AI  TF   ++P  EHYSK  V A     + PDF  WKH  A V FD + +      + + + +   ++I+ M  E  Q F A F+PT         D E +  F ED  YE+ L+REY + +++  +   + + FI++  +  + YNE++  V++ ++ K+       + K+KL + +R  +++E K    R + L
Sbjct:   19 FMLKPRFTNTVPDVPFDAKFMTCPFVPLGRFVEFQPAAIYRDYKHAVICDDDMGLNVDLIDLKKYDEDPIETEIDEKDNILLEDD------GAAKLIAKRSQQHSKLVPWMRKTEYISTEFNRFGVTADRQETKLGYNLKKNQQVEDMYRDKQSQIDAINKTFEDVRKPVKEHYSKKGVKAVEESFVFPDFDHWKHLFAHVQFDGDTITTEFEEEDERQQARESSVIKAMEFED-QKFAAVFVPTIGCLTSFMDDLELERPFDEDMKYEFLLSREYTFKMEH--LPPRDRDVFIMYHRNNVFQYNEVDCNVKMTRKPKMA-----LSRKSKLTLTYRNPSELEQKDMNKREAEL 358
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Fly
Match: atms (gene:FBgn0010750 transcript:FBtr0078868)

HSP 1 Score: 337.035 bits (863), Expect = 3.259e-110
Identity = 194/373 (52.01%), Postives = 252/373 (67.56%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSN-KYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPL--GRNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
            M PTI    +N   E + +R  +R   +IC+VKY N LPDIPFD KFLQY F + RF++YNPTSLERN+K+D+LTEHD+GV VDLI+  ++Q +S   L P DEKLLE++  +  DS RSR H++ VSW+RK+EYIS E +  +    +N E+++GY  K  ++E+ +Y DR++QI+AIE TF+  K    +HYSKPNVV   + PI PDF  WK PCAQVIFD +P   G+N     +  ++A+IRG++DESG+ FVAYFLPTE T  K + D     L+ E++ YEY +AREYNWNVK K    YEENYF V R DG YYNELETRVRLNKRR K+G         TKL+VKHR  + ME + Q  R   L
Sbjct:    1 MPPTINNSAVNSAAEKRPQRQTERKSEIICRVKYGNNLPDIPFDLKFLQYPFDSHRFVQYNPTSLERNFKYDVLTEHDLGVTVDLINRELYQADSMTLLDPADEKLLEEETLTPTDSVRSRQHSRTVSWLRKSEYISTEQTRFQPQNLENIEAKVGYNVKKSLREETLYLDREAQIKAIEKTFSDTKSEITKHYSKPNVVPVEVLPIFPDFTNWKFPCAQVIFDSDPAPAGKNVPAQLEEMSQAMIRGVMDESGEQFVAYFLPTEQTLEKRRTDFINGELYKEEEEYEYKIAREYNWNVKTKASKGYEENYFFVMRQDGIYYNELETRVRLNKRRVKVGQQPN----NTKLVVKHRPLDSMEHRMQRYRERQL 369
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Fly
Match: atms (gene:FBgn0010750 transcript:FBtr0334899)

HSP 1 Score: 337.035 bits (863), Expect = 3.259e-110
Identity = 194/373 (52.01%), Postives = 252/373 (67.56%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSN-KYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPL--GRNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
            M PTI    +N   E + +R  +R   +IC+VKY N LPDIPFD KFLQY F + RF++YNPTSLERN+K+D+LTEHD+GV VDLI+  ++Q +S   L P DEKLLE++  +  DS RSR H++ VSW+RK+EYIS E +  +    +N E+++GY  K  ++E+ +Y DR++QI+AIE TF+  K    +HYSKPNVV   + PI PDF  WK PCAQVIFD +P   G+N     +  ++A+IRG++DESG+ FVAYFLPTE T  K + D     L+ E++ YEY +AREYNWNVK K    YEENYF V R DG YYNELETRVRLNKRR K+G         TKL+VKHR  + ME + Q  R   L
Sbjct:    1 MPPTINNSAVNSAAEKRPQRQTERKSEIICRVKYGNNLPDIPFDLKFLQYPFDSHRFVQYNPTSLERNFKYDVLTEHDLGVTVDLINRELYQADSMTLLDPADEKLLEEETLTPTDSVRSRQHSRTVSWLRKSEYISTEQTRFQPQNLENIEAKVGYNVKKSLREETLYLDREAQIKAIEKTFSDTKSEITKHYSKPNVVPVEVLPIFPDFTNWKFPCAQVIFDSDPAPAGKNVPAQLEEMSQAMIRGVMDESGEQFVAYFLPTEQTLEKRRTDFINGELYKEEEEYEYKIAREYNWNVKTKASKGYEENYFFVMRQDGIYYNELETRVRLNKRRVKVGQQPN----NTKLVVKHRPLDSMEHRMQRYRERQL 369
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Zebrafish
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:553020])

HSP 1 Score: 201.83 bits (512), Expect = 1.200e-59
Identity = 123/276 (44.57%), Postives = 165/276 (59.78%), Query Frame = 3 
Query:  333 LHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKG--DGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSMLE 1145
            L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++     G D  ++A+IR   D+    ++     T         D  E +      VYEY +AREYNWNVKNK    YEENYF +FR +DG YYNELETRVRL+KRR K+G  ++   +   L+ KHR+ N+ E +AQ  R + LE
Sbjct:    3 LDPADEKLLEEEIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKVEVKIGVSVKQQFTEEEIYKDRDSQIAAIEKTFEDAQKSISQHYSKPRVTPVEVLPVFPDFKMWINPCAQVIFDSDPAPKDVSAPAGVDMMSQAMIRYDDDDDDDVYIHTHTHTHTRLTIHCDDVCEAVCNVCVCVYEYKIAREYNWNVKNKASKGYEENYFFIFRDADGVYYNELETRVRLSKRRAKVGAQSSTNAV---LVCKHRDMNEKELEAQEARKAQLE 275
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Xenopus
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:394974])

HSP 1 Score: 339.732 bits (870), Expect = 2.709e-111
Identity = 184/375 (49.07%), Postives = 242/375 (64.53%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPD--SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
            MAPTIQ         ++       P+   ++C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L   DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+D+Y+DR SQI AIE TF  A++P  +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE + +  +D+Y+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+VKHR+ ++ E +AQ  R + L
Sbjct:    1 MAPTIQTQ-AQREDGHRSSSHRTVPERSGVVCRVKYCNTLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVTLDIADEKLLEEEIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKPEVKIGVSVKQQFTEEDIYKDRDSQISAIEKTFEDAQKPISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDASGSAALDMMSQAMIRGMMDEEGNQFVAYFLPGEETMRKRKRDQEEGLDYMPEDIYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRVKAGVQSGTNAV---LVVKHRDMHEKELEAQEARRAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Xenopus
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:394974])

HSP 1 Score: 338.191 bits (866), Expect = 7.654e-111
Identity = 177/347 (51.01%), Postives = 232/347 (66.86%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L   DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+D+Y+DR SQI AIE TF  A++P  +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE + +  +D+Y+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+VKHR+ ++ E +AQ  R + L
Sbjct:   19 GVVCRVKYCNTLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVTLDIADEKLLEEEIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKPEVKIGVSVKQQFTEEDIYKDRDSQISAIEKTFEDAQKPISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDASGSAALDMMSQAMIRGMMDEEGNQFVAYFLPGEETMRKRKRDQEEGLDYMPEDIYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRVKAGVQSGTNAV---LVVKHRDMHEKELEAQEARRAQL 362
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Xenopus
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:394974])

HSP 1 Score: 335.109 bits (858), Expect = 1.163e-109
Identity = 176/347 (50.72%), Postives = 231/347 (66.57%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L   DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+D+Y+DR SQI AIE TF  A++P  +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE + +  +D+Y+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+VKHR+ ++ E +AQ+     L
Sbjct:   21 GVVCRVKYCNTLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVTLDIADEKLLEEEIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKPEVKIGVSVKQQFTEEDIYKDRDSQISAIEKTFEDAQKPISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDASGSAALDMMSQAMIRGMMDEEGNQFVAYFLPGEETMRKRKRDQEEGLDYMPEDIYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRVKAGVQSGTNAV---LVVKHRDMHEKELEAQSSCFLFL 364
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Mouse
Match: Paf1 (Paf1, RNA polymerase II complex component [Source:MGI Symbol;Acc:MGI:1923988])

HSP 1 Score: 341.273 bits (874), Expect = 9.637e-112
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Match: sp|Q4U0S5|PAF1_DANRE (RNA polymerase II-associated factor 1 homolog OS=Danio rerio OX=7955 GN=paf1 PE=1 SV=1)

HSP 1 Score: 342.043 bits (876), Expect = 1.138e-111
Identity = 184/376 (48.94%), Postives = 247/376 (65.69%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPD--SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGD--GKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSMLE 1145
            MAPTIQ         ++       P+   ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KH+LLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++     G D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  ++VYEY +AREYNWNVKNK    YEENYF +FR +DG YYNELETRVRL+KRR K+G  ++   +   L+ KHR+ N+ E +AQ  R + LE
Sbjct:    1 MAPTIQTQ-AQREDGHRSSAHRTVPERSGVVCRVKYGNSLPDIPFDPKFITYPFDQHRFVQYKATSLEKQHKHELLTEPDLGVTIDLINPDTYRIDPNILLDPADEKLLEEEIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKVEVKIGVSVKQQFTEEEIYKDRDSQIAAIEKTFEDAQKSISQHYSKPRVTPVEVLPVFPDFKMWINPCAQVIFDSDPAPKDVSAPAGVDMMSQAMIRGMMDEEGNQFVAYFLPNEDTMRKRKRDVEEELDYMPEEVYEYKIAREYNWNVKNKASKGYEENYFFIFRDADGVYYNELETRVRLSKRRAKVGAQSSTNAV---LVCKHRDMNEKELEAQEARKAQLE 372
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Match: sp|Q5RAX0|PAF1_PONAB (RNA polymerase II-associated factor 1 homolog OS=Pongo abelii OX=9601 GN=PAF1 PE=2 SV=1)

HSP 1 Score: 342.043 bits (876), Expect = 3.054e-111
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Match: sp|Q8N7H5|PAF1_HUMAN (RNA polymerase II-associated factor 1 homolog OS=Homo sapiens OX=9606 GN=PAF1 PE=1 SV=2)

HSP 1 Score: 341.658 bits (875), Expect = 4.339e-111
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Match: sp|Q2KJ14|PAF1_BOVIN (RNA polymerase II-associated factor 1 homolog OS=Bos taurus OX=9913 GN=PAF1 PE=2 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 4.976e-111
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Match: sp|Q8K2T8|PAF1_MOUSE (RNA polymerase II-associated factor 1 homolog OS=Mus musculus OX=10090 GN=Paf1 PE=1 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 6.741e-111
Identity = 178/347 (51.30%), Postives = 233/347 (67.15%), Query Frame = 3 
Query:  117 SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGK--DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
             ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ +KHDLLTE D+GV +DLI+P  ++I+    L P DEKLLE++  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  ++       +  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    L   L+VKHR+ N+ E +AQ  R + L
Sbjct:   28 GVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNAL---LVVKHRDMNEKELEAQEARKAQL 371
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Match: A0A4S2LKU0 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006484 PE=4 SV=1)

HSP 1 Score: 417.157 bits (1071), Expect = 2.735e-137
Identity = 215/359 (59.89%), Postives = 270/359 (75.21%), Query Frame = 3 
Query:  114 DSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDG--KDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXL---------KTKLLVKHREFNDMEFKAQADRLSMLEQEI 1154
            +SL+C++KY NTLP++PFDPKFL Y     RF++Y  TSLERNYKH+LLTE DVGV+VDLIDP +F+I+   KLHPDDE+LLEDD  +A+++R+S  H K VSW+R+TEYIS E  N++ K+D  ES++GY  K  + E+ VYRDR+SQI AIEATF AAK+P  +HYSKPNV A  + P+LPDF LW++PCAQVIFDD+P  +NK +   K+  N+A+IRGMVDESG HFVAYFLPTE+TK   + D E  + +TE   YEY LAREYNWNVKNK M+NYEENYF VFR DG +YNELETRVRL+KRRK+  + +             KT+L+V HR+F D E KAQ DRL MLE +I
Sbjct:   28 ESLLCRLKYRNTLPELPFDPKFLAYPLEPSRFLQYVATSLERNYKHELLTETDVGVDVDLIDPDVFKIDKSVKLHPDDERLLEDDTPAAINARKS-RHQKSVSWLRRTEYISTEMYNRWNKSDKVESKLGYSVKRHLNEEIVYRDRESQIAAIEATFKAAKKPITKHYSKPNVHAVEVLPVLPDFTLWRYPCAQVIFDDDPARKNKSNAEQKEEVNQAMIRGMVDESGDHFVAYFLPTESTKQLRRLDIENHVPYTEGAAYEYELAREYNWNVKNKTMANYEENYFFVFRKDGVFYNELETRVRLSKRRKLTQSGSFMTGGGGGALPAPKTRLIVHHRDFTDEELKAQTDRLYMLEHDI 385
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Match: A0A5J4NGT4 (RNA polymerase II-associated factor 1 (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0004785 PE=4 SV=1)

HSP 1 Score: 414.075 bits (1063), Expect = 8.627e-137
Identity = 219/371 (59.03%), Postives = 272/371 (73.32%), Query Frame = 3 
Query:  111 PDSLICKVKYTNTLPDIPFDPKFLQYSFGNER-----------FIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDG--KDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXL---------KTKLLVKHREFNDMEFKAQADRLSMLEQEI 1154
            P+SL+C++KY N LP++PFDPKFL Y     R           F++Y  TSLERNYKH+LLTE DVGVEVDLIDP IF+I+   KLHPDDE+LLED+  +A+++R+S  H K VSW+R+TEYIS E  N++ K+D  ES++GY  K  + E+ VYRDR+SQI AIEATF AAK+P ++HYSKPNV A  + P+LPDF LW++PCAQVIFDD+P  +NK +   K+  N+A+IRGMVDESG HFVAYFLPTE+TK   + DAE  I +TE   YEY LAREYNWNVKNK M+NYEENYF VFR DG YYNELETRVRL+KRRK+  + +             KT+L+V HR+F D E KAQ DRL MLE +I
Sbjct:   53 PESLLCRLKYKNALPELPFDPKFLAYPLEPSRYAYVQSNFFRRFLQYVATSLERNYKHELLTETDVGVEVDLIDPDIFKIDKSVKLHPDDERLLEDENPAAINARKS-RHQKSVSWLRRTEYISTELYNRWNKSDKVESKLGYSVKRHLNEEIVYRDRESQIAAIEATFKAAKKPIVKHYSKPNVHAVEVLPVLPDFALWRYPCAQVIFDDDPARKNKSNAEQKEEVNQAMIRGMVDESGDHFVAYFLPTESTKQLRRLDAENRIPYTEGAAYEYELAREYNWNVKNKTMANYEENYFFVFRKDGVYYNELETRVRLSKRRKLTQSGSFVTGGGGGVPSAPKTRLIVHHRDFTDDELKAQTDRLYMLEHDI 422
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Match: A0A3R7EQU6 (RNA polymerase II-associated factor 1-like protein OS=Clonorchis sinensis OX=79923 GN=PAF1 PE=4 SV=1)

HSP 1 Score: 415.616 bits (1067), Expect = 2.016e-134
Identity = 214/361 (59.28%), Postives = 270/361 (74.79%), Query Frame = 3 
Query:  114 DSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDG--KDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXL-----------KTKLLVKHREFNDMEFKAQADRLSMLEQEI 1154
            +SL+C++KY NTLP++PFDPKFL Y     RF++Y  TSLERNYKH+LLTE DVGV+VDLIDP +F+I+   KLHPDDE+LLEDD  +A+++R+S  H K VSW+R+TEYIS E  N++ K+D  ES++GY  K  + E+ VYRDR+SQI AIEATF AAK+P  +HYSKPNV A  + P+LPDF LW++PCAQVIFDD+P  +NK +   K+  N+A+IRGMVDESG HFVAYFLPTE+TK   + D E  + +TE   YEY LAREYNWNVKNK M+NYEENYF VFR DG +YNELETRVRL+KRRK+  + +               KT+L+V HR+F D E KAQ DRL MLE ++
Sbjct:  206 ESLLCRLKYRNTLPELPFDPKFLAYPLEPSRFLQYVATSLERNYKHELLTETDVGVDVDLIDPDVFKIDKSVKLHPDDERLLEDDTPAAINARKS-RHQKSVSWLRRTEYISTEMYNRWNKSDKVESKLGYSVKRHLNEEIVYRDRESQIAAIEATFRAAKKPITKHYSKPNVHAVEVLPVLPDFTLWRYPCAQVIFDDDPARKNKSNAEQKEEVNQAMIRGMVDESGDHFVAYFLPTESTKQLRRLDIENHVPYTEGAAYEYELAREYNWNVKNKTMANYEENYFFVFRKDGVFYNELETRVRLSKRRKLTQSGSFMTGGGGGGGALPAPKTRLIVHHRDFTDEELKAQTDRLYMLEHDV 565
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Match: A0A5K4F1R0 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 404.831 bits (1039), Expect = 6.014e-134
Identity = 212/350 (60.57%), Postives = 262/350 (74.86%), Query Frame = 3 
Query:  114 DSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKG--DGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXL----KTKLLVKHREFNDMEFKAQADRLSML 1142
            +SL+C++KY N LP++PFDPKFL Y     RF++Y  TSLERNYKH+LLTE DVGVEVDLIDP +F+I+    LHPDDE+LLED+A + +++R+SRH  K VSW+RKTEYIS E  N++ K++  ESR+GY  K  + E+ VYRDR+SQI AIE TF AA++P  +HYSKPNV A  + P+LPDF LW++PCAQVIFDD+P  +NK   + K+  N+A+IRGMVDESG HFVAYFLPTE TK   + DAE    +TED  YEY L REYNWNVKNK M+NYEENYF  FR DG YYNELETRVRL+KRRK+  +          KT+L+V HR+F D E KAQ DRL ML
Sbjct:   27 ESLLCRLKYQNNLPELPFDPKFLVYPLEPSRFLQYVATSLERNYKHELLTETDVGVEVDLIDPDVFRIDKRATLHPDDERLLEDEAPTFVNARKSRHQ-KSVSWLRKTEYISTELYNRWNKSEKVESRLGYNVKRHLNEEIVYRDRESQINAIEETFKAAEKPIHKHYSKPNVHALEVLPVLPDFTLWRYPCAQVIFDDDPSRKNKTTTEQKEEVNQAMIRGMVDESGDHFVAYFLPTEQTKQLRRLDAENQTPYTEDAAYEYELTREYNWNVKNKTMANYEENYFFCFRKDGVYYNELETRVRLSKRRKLNQSGTNVGALQAPKTRLIVHHRDFTDEELKAQTDRLYML 375
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Match: C1LET2 (Putative DNA helicase INO80 OS=Schistosoma japonicum OX=6182 GN=EWB00_004165 PE=2 SV=1)

HSP 1 Score: 404.06 bits (1037), Expect = 1.936e-133
Identity = 211/350 (60.29%), Postives = 264/350 (75.43%), Query Frame = 3 
Query:  114 DSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKG--DGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXL----KTKLLVKHREFNDMEFKAQADRLSML 1142
            +SL+C++KY N LP++PFDPKFL Y     RF++Y  TSLERNYKH+LLTE DVGVEVDLIDP +F+I+ +  LHPDDE+LLED+A + +++R+SRH  K VSW+RKTEYIS E  N++ K++  ESR+GY  K  + E+ VYRDR+SQI AIE TF AA++P  +HYSKPNV A  + P+LPDF LW++PCAQVIFDD+P  +NK   + K+  N+A+IRGMVDESG HFVAYFLPTE TK   + DAE  + +TE   YEY L REYNWNVKNK M+NYEENYF  FR DG YYNELETRVRL+KRRK+  +     +    KT+L+V HR+F D E KAQ DRL ML
Sbjct:   27 ESLLCRLKYQNNLPELPFDPKFLVYPLEPSRFLQYVATSLERNYKHELLTETDVGVEVDLIDPDVFRIDKKATLHPDDERLLEDEAPTFVNARKSRHQ-KSVSWLRKTEYISTELYNRWNKSEKVESRLGYNVKRHLNEEIVYRDRESQINAIEETFNAARKPIHKHYSKPNVHALEVLPVLPDFTLWRYPCAQVIFDDDPSRKNKTTTEQKEEVNQAMIRGMVDESGDHFVAYFLPTEQTKQLRRLDAENRLPYTEGAAYEYELTREYNWNVKNKTMANYEENYFFCFRKDGVYYNELETRVRLSKRRKLNQSGTNVGVLQAPKTRLIVHHRDFTDEELKAQTDRLYML 375
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Cavefish
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:103033554])

HSP 1 Score: 334.339 bits (856), Expect = 2.147e-109
Identity = 182/376 (48.40%), Postives = 243/376 (64.63%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPD--SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGR--NKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSMLE 1145
            MAPTIQ         ++       P+   ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ ++H+LLTE D+GV +DLI+P  ++I+    L P DEKLLE+D  +   S+RS+ H K V WMRKTEYIS E +      D  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  +  +     D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  +++Y+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+ KHR+ N+ E +AQ  R + LE
Sbjct:    1 MAPTIQTQ-AQREDGHRSSAHRAIPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQHRFVQYKATSLEKQHRHELLTEPDLGVTIDLINPDTYRIDPNILLDPADEKLLEEDIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNDKVEVKIGVSVKQQFTEEEIYKDRDSQIAAIEKTFEDAQKNITQHYSKPRVTPVEVLPVFPDFKMWINPCAQVIFDSDPAPKEVSGSAAVDMMSQAMIRGMMDEEGNQFVAYFLPNEDTLRKRKRDVEEEMDYMPEELYDYKIAREYNWNVKNKASKGYEENYFFIFRDGDGVYYNELETRVRLSKRRAKAGAQSTTNAV---LVCKHRDMNEKELEAQEARKAQLE 372
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Cavefish
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:103033554])

HSP 1 Score: 330.102 bits (845), Expect = 3.157e-108
Identity = 179/366 (48.91%), Postives = 239/366 (65.30%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGR--NKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQ 1121
            MAPTIQ          + +R +     ++C+VKY N+LPDIPFDPKF+ Y F   RF++Y  TSLE+ ++H+LLTE D+GV +DLI+P  ++I+    L P DEKLLE+D  +   S+RS+ H K V WMRKTEYIS E +      D  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    + P+ PDFK+W +PCAQVIFD +P  +  +     D  ++A+IRGM+DE G  FVAYFLP E T  K KRD EE++ +  +++Y+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+ KHR+ N+ E +AQ
Sbjct:    1 MAPTIQ---------TQAQREDGHRSGVVCRVKYCNSLPDIPFDPKFITYPFDQHRFVQYKATSLEKQHRHELLTEPDLGVTIDLINPDTYRIDPNILLDPADEKLLEEDIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNDKVEVKIGVSVKQQFTEEEIYKDRDSQIAAIEKTFEDAQKNITQHYSKPRVTPVEVLPVFPDFKMWINPCAQVIFDSDPAPKEVSGSAAVDMMSQAMIRGMMDEEGNQFVAYFLPNEDTLRKRKRDVEEEMDYMPEELYDYKIAREYNWNVKNKASKGYEENYFFIFRDGDGVYYNELETRVRLSKRRAKAGAQSTTNAV---LVCKHRDMNEKELEAQ 354
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Sea Lamprey
Match: PAF1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:HGNC Symbol;Acc:HGNC:25459])

HSP 1 Score: 178.718 bits (452), Expect = 5.612e-54
Identity = 104/212 (49.06%), Postives = 132/212 (62.26%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDS-LICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFK 668
            MAPTIQ    +               S L+C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KH+LLTE D+GV +DLI+P  ++I+    L P DEKLLE++ ++   SRRS+ H K V WMRKTEYIS E +      +  E +IG   K    EDD+Y+DR SQI AIE TF  AK+    HYSKP VV   + P+ PDFK
Sbjct:    1 MAPTIQSAQRDAESSRSSSHRGAPERSGLVCRVKYCNTLPDIPFDPKFITYPFEANRFVQYKATSLEKQHKHELLTEPDLGVTIDLINPDTYKIDPNAYLDPADEKLLEEEIATPQGSRRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEIKIGVSVKQQFTEDDIYKDRDSQISAIEKTFEDAKKEIEAHYSKPRVVPVEVMPVFPDFK 212
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Nematostella
Match: EDO42996 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZS5])

HSP 1 Score: 335.495 bits (859), Expect = 1.381e-112
Identity = 177/339 (52.21%), Postives = 233/339 (68.73%), Query Frame = 3 
Query:  120 LICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRN-KGDGK-DSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRRKIGPXXXXXXLKTKLLVKHREFNDMEFKAQA 1124
             + ++KY N LPDIPFD KF+ Y F   RF++Y PTSLE+NYKH+LLT+ D+GV +DLIDP ++ ++    L P DEKLLE+DA    D +R+RHHNK VSW+RKTEYIS E++   T  + +E+++G+  K   K  D+Y+DR SQ+ AIE TF AAK+P   H SKPN+    + P+ PDF++W +PCA V+FD +P  R+ KG  + D  ++A+IRGMVD SG  FVAYFLP + T  K KRD EE   + +++ Y Y +AREYNWNVKNK    YEENYF VFR  +G +YNEL TRVRL+KRR  G      +  +KL+VKHRE ND E  AQ 
Sbjct:    7 FVTRIKYNNNLPDIPFDAKFISYPFEGNRFVQYKPTSLEKNYKHELLTDIDLGVNIDLIDPQMYAVDPNATLDPADEKLLEEDAVVMPDKKRTRHHNKNVSWLRKTEYISTEYNRAQTSNEMAETKVGFSIKKKFKGTDLYKDRDSQLAAIENTFEAAKKPIHHHPSKPNLTPVEVLPVFPDFQMWGYPCAHVVFDTDPAPRDRKGPAQVDEMSQAMIRGMVDASGDQFVAYFLPDKETLNKRKRDHEEMADYQDEEEYVYKMAREYNWNVKNKSTKGYEENYFFVFRDGEGVFYNELVTRVRLSKRRARGGVGGVRSAVSKLVVKHRELNDGEKNAQV 345
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Nematostella
Match: EDO42995 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZS4])

HSP 1 Score: 60.4622 bits (145), Expect = 2.739e-10
Identity = 24/46 (52.17%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:  183 QYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQIN 320
            Q++    RF++Y PTSLE+NYKH+LLT+ D+GV +DLIDP ++ ++
Sbjct:   17 QHAARTTRFVQYKPTSLEKNYKHELLTDIDLGVNIDLIDPQMYAVD 62
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Medaka
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:101165083])

HSP 1 Score: 344.354 bits (882), Expect = 4.149e-113
Identity = 186/375 (49.60%), Postives = 244/375 (65.07%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPD--SLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGR--NKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELETRVRLNKRR-KIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSML 1142
            MAPTIQ      +  ++       P+   ++C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KH+LLTE D+GV +DLI+P  ++I+    L P DEKLLE+D  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    I P+ PDFK+W +PCAQVIFD +P  +  +   G +  ++A+IRGM+DE G  FVAYFLP E T  K KRD+EE + +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELETRVRL+KRR K G  +    +   L+ KHR+ N+ E +AQ  R + L
Sbjct:    1 MAPTIQTQAQRDDGHSRPSSHRTVPERSGIVCRVKYCNTLPDIPFDPKFITYPFDQHRFVQYKATSLEKQHKHELLTEPDLGVTIDLINPDTYRIDPTILLDPADEKLLEEDIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKVEVKIGVSVKQQFTEEEIYKDRDSQISAIEKTFEDAQKSIAQHYSKPRVTPVEILPVFPDFKMWINPCAQVIFDSDPAPKDISAPAGVEMMSQAMIRGMMDEEGNQFVAYFLPHEETLRKRKRDSEEGVEYMADDVYDYKIAREYNWNVKNKASKGYEENYFFIFRDGDGVYYNELETRVRLSKRRAKAGAQSTTNAV---LVCKHRDMNEKELEAQEARKAQL 372
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Medaka
Match: paf1 (PAF1 homolog, Paf1/RNA polymerase II complex component [Source:NCBI gene;Acc:101165083])

HSP 1 Score: 310.457 bits (794), Expect = 9.057e-103
Identity = 167/327 (51.07%), Postives = 216/327 (66.06%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYK-KTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGR--NKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFR-SDGTYYNELET 1007
            MAPTIQ          + +R +     ++C+VKY NTLPDIPFDPKF+ Y F   RF++Y  TSLE+ +KH+LLTE D+GV +DLI+P  ++I+    L P DEKLLE+D  +   S+RS+ H K V WMRKTEYIS E +      +  E +IG   K    E+++Y+DR SQI AIE TF  A++   +HYSKP V    I P+ PDFK+W +PCAQVIFD +P  +  +   G +  ++A+IRGM+DE G  FVAYFLP E T  K KRD+EE + +  DDVY+Y +AREYNWNVKNK    YEENYF +FR  DG YYNELET
Sbjct:    1 MAPTIQ---------TQAQRDDGHRSGIVCRVKYCNTLPDIPFDPKFITYPFDQHRFVQYKATSLEKQHKHELLTEPDLGVTIDLINPDTYRIDPTILLDPADEKLLEEDIQAPSSSKRSQQHAKVVPWMRKTEYISTEFNRYGVSNEKVEVKIGVSVKQQFTEEEIYKDRDSQISAIEKTFEDAQKSIAQHYSKPRVTPVEILPVFPDFKMWINPCAQVIFDSDPAPKDISAPAGVEMMSQAMIRGMMDEEGNQFVAYFLPHEETLRKRKRDSEEGVEYMADDVYDYKIAREYNWNVKNKASKGYEENYFFIFRDGDGVYYNELET 318
[Close]
BLAST of RNA polymerase II-associated factor 1 vs. Planmine SMEST
Match: SMESG000006276.1 (SMESG000006276.1)

HSP 1 Score: 780.015 bits (2013), Expect = 0.000e+0
Identity = 414/414 (100.00%), Postives = 414/414 (100.00%), Query Frame = 3 
Query:   39 MAPTIQKDDINFXXXXXXXXXXXXPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKXXXXXXXXXXXXXXXXHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYKKTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPXXXXXXLKTKLLVKHREFNDMEFKAQADRLSMLEQEIDEEMSDGEDNQNNQAVNESEAYADGKEQKDLSDTDSDVPKKK 1280
            MAPTIQKDDINFNKENKEKRVEKRPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKLLEDDASSALDSRRSRHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYKKTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPNAAAANLKTKLLVKHREFNDMEFKAQADRLSMLEQEIDEEMSDGEDNQNNQAVNESEAYADGKEQKDLSDTDSDVPKKK
Sbjct:    1 MAPTIQKDDINFNKENKEKRVEKRPDSLICKVKYTNTLPDIPFDPKFLQYSFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLHPDDEKLLEDDASSALDSRRSRHHNKYVSWMRKTEYISNEHSNKYTKTDNSESRIGYKKTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQNIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGKDSYNRALIRGMVDESGQHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGMSNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPNAAAANLKTKLLVKHREFNDMEFKAQADRLSMLEQEIDEEMSDGEDNQNNQAVNESEAYADGKEQKDLSDTDSDVPKKK 414
The following BLAST results are available for this feature:
BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
PAF19.034e-11251.30PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
PAF11.740e-11149.60PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
PAF11.439e-6850.86PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pafo-11.308e-4732.49RNA polymerase II-associated factor 1 homolog [So... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
atms3.259e-11052.01gene:FBgn0010750 transcript:FBtr0078868[more]
atms3.259e-11052.01gene:FBgn0010750 transcript:FBtr0334899[more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
paf11.200e-5944.57PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
paf12.709e-11149.07PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
paf17.654e-11151.01PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
paf11.163e-10950.72PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Paf19.637e-11251.30Paf1, RNA polymerase II complex component [Source:... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q4U0S5|PAF1_DANRE1.138e-11148.94RNA polymerase II-associated factor 1 homolog OS=D... [more]
sp|Q5RAX0|PAF1_PONAB3.054e-11151.30RNA polymerase II-associated factor 1 homolog OS=P... [more]
sp|Q8N7H5|PAF1_HUMAN4.339e-11151.30RNA polymerase II-associated factor 1 homolog OS=H... [more]
sp|Q2KJ14|PAF1_BOVIN4.976e-11151.30RNA polymerase II-associated factor 1 homolog OS=B... [more]
sp|Q8K2T8|PAF1_MOUSE6.741e-11151.30RNA polymerase II-associated factor 1 homolog OS=M... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LKU02.735e-13759.89Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A5J4NGT48.627e-13759.03RNA polymerase II-associated factor 1 (Fragment) O... [more]
A0A3R7EQU62.016e-13459.28RNA polymerase II-associated factor 1-like protein... [more]
A0A5K4F1R06.014e-13460.57Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
C1LET21.936e-13360.29Putative DNA helicase INO80 OS=Schistosoma japonic... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
paf12.147e-10948.40PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
paf13.157e-10848.91PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PAF15.612e-5449.06PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO429961.381e-11252.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429952.739e-1052.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
paf14.149e-11349.60PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
paf19.057e-10351.07PAF1 homolog, Paf1/RNA polymerase II complex compo... [more]
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BLAST of RNA polymerase II-associated factor 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000006276.10.000e+0100.00SMESG000006276.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033043 ID=SMED30033043|Name=RNA polymerase II-associated factor 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1410bp
ATTACATTCAATTAATATTTAATATATAGGTTTTTGGTATGGCTCCTACG
ATTCAAAAAGACGACATTAACTTTAACAAAGAAAATAAAGAAAAACGTGT
TGAGAAACGACCAGATTCACTTATATGCAAAGTTAAATACACAAATACAT
TACCGGATATTCCATTCGATCCTAAGTTTTTACAATACTCTTTCGGAAAT
GAAAGATTTATTAAATATAATCCCACATCATTGGAAAGGAATTACAAGCA
TGACCTTTTAACAGAGCATGACGTCGGCGTAGAAGTAGATCTAATTGACC
CTCTAATTTTTCAGATAAATTCTGAAGACAAATTACATCCAGACGATGAA
AAGTTATTAGAAGACGATGCTTCTAGTGCCTTAGATTCTAGGAGATCAAG
ACATCACAACAAATATGTTTCATGGATGAGAAAAACCGAATATATTTCTA
ATGAACATTCTAATAAATATACTAAAACAGATAATTCTGAATCTAGAATT
GGTTATAAAAAAACATTGATGAAAGAAGATGATGTTTACCGAGACAGAAA
AAGTCAAATCGAAGCTATTGAAGCAACATTTGCAGCAGCTAAGGAGCCCT
TCATTGAACATTACAGCAAGCCTAATGTTGTTGCCCAAAATATCTATCCT
ATTTTGCCTGACTTTAAGTTATGGAAACATCCATGTGCTCAGGTCATTTT
TGATGATAACCCACTCGGTCGCAATAAAGGTGATGGCAAGGATAGTTATA
ATCGCGCTCTTATTAGGGGTATGGTAGATGAATCTGGGCAGCATTTCGTA
GCATATTTCCTCCCAACTGAAGCAACCAAGGGCAAATTGAAGCGTGATGC
TGAAGAGGACATCTTATTCACTGAGGACGATGTTTATGAATATTACCTAG
CCCGCGAGTATAACTGGAACGTTAAAAACAAAGGAATGTCAAATTATGAG
GAAAACTATTTTATAGTGTTTCGATCAGATGGTACATATTACAATGAACT
GGAAACCAGGGTCAGGCTGAATAAGCGGCGTAAAATTGGACCAAACGCCG
CCGCAGCGAACTTGAAAACGAAACTGTTGGTAAAACATCGGGAATTCAAC
GACATGGAATTCAAAGCTCAAGCCGACAGACTGTCGATGCTGGAACAGGA
AATTGACGAGGAAATGAGCGACGGAGAGGACAATCAGAACAATCAAGCGG
TGAATGAATCTGAAGCATATGCCGATGGCAAAGAACAGAAAGATCTGTCT
GACACGGATTCTGATGTTCCGAAGAAGAAAGAATCACCAGCAGCATCAAT
GTCCGATTCGGACAGTGAAAGTTCAATTATATAGAAGAAGAGCTTTACTA
ACATATTTTGTAACAATAGATTCTGTTTTGTTTTGAGCTGGTAGGCGATC
CAGATCGGAA
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protein sequence of SMED30033043-orf-1

>SMED30033043-orf-1 ID=SMED30033043-orf-1|Name=SMED30033043-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=432bp
MAPTIQKDDINFNKENKEKRVEKRPDSLICKVKYTNTLPDIPFDPKFLQY
SFGNERFIKYNPTSLERNYKHDLLTEHDVGVEVDLIDPLIFQINSEDKLH
PDDEKLLEDDASSALDSRRSRHHNKYVSWMRKTEYISNEHSNKYTKTDNS
ESRIGYKKTLMKEDDVYRDRKSQIEAIEATFAAAKEPFIEHYSKPNVVAQ
NIYPILPDFKLWKHPCAQVIFDDNPLGRNKGDGKDSYNRALIRGMVDESG
QHFVAYFLPTEATKGKLKRDAEEDILFTEDDVYEYYLAREYNWNVKNKGM
SNYEENYFIVFRSDGTYYNELETRVRLNKRRKIGPNAAAANLKTKLLVKH
REFNDMEFKAQADRLSMLEQEIDEEMSDGEDNQNNQAVNESEAYADGKEQ
KDLSDTDSDVPKKKESPAASMSDSDSESSII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR007133RNA_pol_II-assoc_Paf1
Vocabulary: biological process
TermDefinition
GO:0016570histone modification
GO:0006368transcription elongation from RNA polymerase II promoter
Vocabulary: cellular component
TermDefinition
GO:0016593Cdc73/Paf1 complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007133RNA polymerase II associated factor Paf1PFAMPF03985Paf1coord: 28..426
e-value: 3.3E-129
score: 431.6
IPR007133RNA polymerase II associated factor Paf1PANTHERPTHR23188FAMILY NOT NAMEDcoord: 21..416
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 394..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..431
NoneNo IPR availablePANTHERPTHR23188:SF14RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOGcoord: 21..416