Cytosolic purine 5' nucleotidase

Overview
NameCytosolic purine 5' nucleotidase
Smed IDSMED30032987
Length (bp)1821
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cytosolic purine 5' nucleotidase (SMED30032987) t-SNE clustered cells

Violin plots show distribution of expression levels for Cytosolic purine 5' nucleotidase (SMED30032987) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cytosolic purine 5' nucleotidase (SMED30032987) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cytosolic purine 5' nucleotidase (SMED30032987) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032987

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30032987SMESG000009496.1 Contig55190newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032987SMESG000009496.1 Contig55190uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032987SMESG000009496.1 Contig42629newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032987SMESG000009496.1 Contig42629uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
testisSMED30032987SMESG000009496.1 PL08003A1D02ncbi_smed_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30032987SMESG000009496.1 PL06009A2B12ncbi_smed_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testis primordiumSMED30032987SMESG000009496.1 PL06009A2B12_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
testis primordiumSMED30032987SMESG000009496.1 PL08003A1D02_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Match: NT5C2 (5'-nucleotidase, cytosolic II [Source:HGNC Symbol;Acc:HGNC:8022])

HSP 1 Score: 376.326 bits (965), Expect = 2.118e-132
Identity = 187/383 (48.83%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 2.118e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Match: NT5C2 (5'-nucleotidase, cytosolic II [Source:HGNC Symbol;Acc:HGNC:8022])

HSP 1 Score: 376.326 bits (965), Expect = 2.118e-132
Identity = 187/383 (48.83%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 2.118e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Match: NT5DC4 (5'-nucleotidase domain containing 4 [Source:HGNC Symbol;Acc:HGNC:27678])

HSP 1 Score: 263.077 bits (671), Expect = 5.698e-81
Identity = 133/303 (43.89%), Postives = 195/303 (64.36%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRI-YVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIK 1276
            +  + +  LS  EI   YP+KF++ D+ +  Y++N LF+L E YL A ++ +F+    Y   + G Q  N+FMS++S+ QDV DA + IH  G LK KTLE LE YV    +LP +L +++  G K FL TNS+Y+YT  IM Y+ S+ +   + R W +YFD IVVD +KP FF  G +LR V+ ++GKL VG + GP +   +YSGGS ++  +L+G RG D+LY+GDHIFGDILKSKK  GWRT LV+PE++ EL +W +E+    EL +LD  L +IY  ++ +S +   +   K +I+
Sbjct:   90 LGAYGFTFLSEAEIWSFYPSKFIQRDDLQCFYILNMLFNLPETYLYACLVDFFSGCSRYTNCDTGYQHGNLFMSFRSLFQDVTDAMNNIHQSGCLK-KTLEDLEKYVKKDPRLPILLGKMKEVG-KVFLATNSSYNYTNAIMTYLFSISEAEASGRPWRSYFDLIVVDTQKPHFFAEGLVLRQVNTDSGKLHVGTYTGPHQHCAVYSGGSSDMVCELLGVRGMDILYIGDHIFGDILKSKKRQGWRTCLVVPELSWELDIWAQEKERLEELKRLDTHLADIYQHMDGSSCELQVINFTKREIQ 390

HSP 2 Score: 100.908 bits (250), Expect = 4.095e-22
Identity = 50/95 (52.63%), Postives = 63/95 (66.32%), Query Frame = 3 
Query:  120 IFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLT 404
            IFVNRSL + KIR +GFDMDYTLA YKSP +E L F+L  + L+  GYP E   + Y+  F  R L FD+ YG  +K D +GN+LL   GF FL+
Sbjct:    5 IFVNRSLALGKIRCFGFDMDYTLAAYKSPAYEALTFELLLERLVCIGYPHEILRYTYDPTFPTRRLVFDELYGNLLKVDAHGNVLLGAYGFTFLS 99
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Match: NT5C2 (5'-nucleotidase, cytosolic II [Source:HGNC Symbol;Acc:HGNC:8022])

HSP 1 Score: 126.331 bits (316), Expect = 1.850e-57
Identity = 68/142 (47.89%), Postives = 93/142 (65.49%), Query Frame = 2 
Query:  377 GFNWILLSH------NEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYT 781
            GFN+I  S        E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT
Sbjct:  124 GFNFIRGSQVAVQKRPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYT 264

HSP 2 Score: 117.857 bits (294), Expect = 1.850e-57
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Match: NT5C2 (5'-nucleotidase, cytosolic II [Source:HGNC Symbol;Acc:HGNC:8022])

HSP 1 Score: 139.813 bits (351), Expect = 2.704e-38
Identity = 63/124 (50.81%), Postives = 89/124 (71.77%), Query Frame = 2 
Query:  905 ILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIK 1276
            +LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK
Sbjct:    1 VLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIK 124
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Match: Y71H10B.1 (pep chromosome:WBcel235:X:2834272:2840292:-1 gene:WBGene00022201.1 transcript:Y71H10B.1b.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H10B.1)

HSP 1 Score: 355.525 bits (911), Expect = 9.090e-125
Identity = 180/414 (43.48%), Postives = 267/414 (64.49%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDY-IVTEK-GVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLR------LNDK-NLMQGNSEDDDDTNS 1606
             L ++EI  +YPNK++   +SR+YV+NTLF++ E +L+ ++I +F  H  Y ++T+K GV+   V MSY+SI QD+R   D++H E  +K++ +E  E YV   ++ P  L +LR  G KTFLLTNS+++YT  +M++IL          +W TYF+ IVVD+ KP++F  G++ R V+ +TG   +G H GPL+ G +YSGGSC+ F K++  RGK+VLYVGDHIFGD+L+SKK  GWRT+LV+PE+ +EL VW   +  + E+  +++TL +IY  L+ AS  KP VK +  KI++L  Q++  YG LGSLFR G R TFF+SQ++RYAD+Y++SC N++HYP  Y FR+    +PHE   + H   +      L       + +R      LND+        ED+D TNS
Sbjct:  108 FLKNHEIEEMYPNKYLPLSDSRVYVLNTLFNMPETHLICQLIDFFDKHPHYSMLTDKTGVKGGEVMMSYKSIFQDLRRVIDWVHVESTMKKQVMENPEKYVIRDERAPRFLSQLREHGKKTFLLTNSDWTYTNVMMKFILG--------DDWKTYFNIIVVDSCKPRWFAEGTVFREVNTDTGSYKLGIHTGPLREGVVYSGGSCDAFQKMMKCRGKEVLYVGDHIFGDVLRSKKSRGWRTFLVVPELVNELTVWTDSKPLFDEMGHMESTLADIYKNLDGASRHKPVVKDIVDKIRKLTNQMDEEYGCLGSLFRTGGRTTFFASQVERYADVYASSCYNLVHYPSFYYFRAPMKCMPHE-LTVDHAHTLRSRSNTLERQQSVGQQVRGWNKKTLNDQETFCHEEEEDEDQTNS 512

HSP 2 Score: 112.079 bits (279), Expect = 9.090e-125
Identity = 50/100 (50.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R+   R+FVNRSL++EKI  +GFDMDYTL  YK+P  E L F++    L++ GYP+E +SFKY+  F +RGLWF+ ++G  +K D +GNIL  + G  FL
Sbjct:   10 RNPAERVFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDTGYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVDGFGNILGGIHGLRFL 109
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Match: Y71H10B.1 (pep chromosome:WBcel235:X:2834224:2840292:-1 gene:WBGene00022201.1 transcript:Y71H10B.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H10B.1)

HSP 1 Score: 355.525 bits (911), Expect = 9.090e-125
Identity = 180/414 (43.48%), Postives = 267/414 (64.49%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDY-IVTEK-GVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLR------LNDK-NLMQGNSEDDDDTNS 1606
             L ++EI  +YPNK++   +SR+YV+NTLF++ E +L+ ++I +F  H  Y ++T+K GV+   V MSY+SI QD+R   D++H E  +K++ +E  E YV   ++ P  L +LR  G KTFLLTNS+++YT  +M++IL          +W TYF+ IVVD+ KP++F  G++ R V+ +TG   +G H GPL+ G +YSGGSC+ F K++  RGK+VLYVGDHIFGD+L+SKK  GWRT+LV+PE+ +EL VW   +  + E+  +++TL +IY  L+ AS  KP VK +  KI++L  Q++  YG LGSLFR G R TFF+SQ++RYAD+Y++SC N++HYP  Y FR+    +PHE   + H   +      L       + +R      LND+        ED+D TNS
Sbjct:  108 FLKNHEIEEMYPNKYLPLSDSRVYVLNTLFNMPETHLICQLIDFFDKHPHYSMLTDKTGVKGGEVMMSYKSIFQDLRRVIDWVHVESTMKKQVMENPEKYVIRDERAPRFLSQLREHGKKTFLLTNSDWTYTNVMMKFILG--------DDWKTYFNIIVVDSCKPRWFAEGTVFREVNTDTGSYKLGIHTGPLREGVVYSGGSCDAFQKMMKCRGKEVLYVGDHIFGDVLRSKKSRGWRTFLVVPELVNELTVWTDSKPLFDEMGHMESTLADIYKNLDGASRHKPVVKDIVDKIRKLTNQMDEEYGCLGSLFRTGGRTTFFASQVERYADVYASSCYNLVHYPSFYYFRAPMKCMPHE-LTVDHAHTLRSRSNTLERQQSVGQQVRGWNKKTLNDQETFCHEEEEDEDQTNS 512

HSP 2 Score: 112.079 bits (279), Expect = 9.090e-125
Identity = 50/100 (50.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R+   R+FVNRSL++EKI  +GFDMDYTL  YK+P  E L F++    L++ GYP+E +SFKY+  F +RGLWF+ ++G  +K D +GNIL  + G  FL
Sbjct:   10 RNPAERVFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDTGYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVDGFGNILGGIHGLRFL 109
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Match: Y71H10B.1 (pep chromosome:WBcel235:X:2834272:2840292:-1 gene:WBGene00022201.1 transcript:Y71H10B.1a.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H10B.1)

HSP 1 Score: 355.525 bits (911), Expect = 2.486e-124
Identity = 180/414 (43.48%), Postives = 267/414 (64.49%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDY-IVTEK-GVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLR------LNDK-NLMQGNSEDDDDTNS 1606
             L ++EI  +YPNK++   +SR+YV+NTLF++ E +L+ ++I +F  H  Y ++T+K GV+   V MSY+SI QD+R   D++H E  +K++ +E  E YV   ++ P  L +LR  G KTFLLTNS+++YT  +M++IL          +W TYF+ IVVD+ KP++F  G++ R V+ +TG   +G H GPL+ G +YSGGSC+ F K++  RGK+VLYVGDHIFGD+L+SKK  GWRT+LV+PE+ +EL VW   +  + E+  +++TL +IY  L+ AS  KP VK +  KI++L  Q++  YG LGSLFR G R TFF+SQ++RYAD+Y++SC N++HYP  Y FR+    +PHE   + H   +      L       + +R      LND+        ED+D TNS
Sbjct:  105 FLKNHEIEEMYPNKYLPLSDSRVYVLNTLFNMPETHLICQLIDFFDKHPHYSMLTDKTGVKGGEVMMSYKSIFQDLRRVIDWVHVESTMKKQVMENPEKYVIRDERAPRFLSQLREHGKKTFLLTNSDWTYTNVMMKFILG--------DDWKTYFNIIVVDSCKPRWFAEGTVFREVNTDTGSYKLGIHTGPLREGVVYSGGSCDAFQKMMKCRGKEVLYVGDHIFGDVLRSKKSRGWRTFLVVPELVNELTVWTDSKPLFDEMGHMESTLADIYKNLDGASRHKPVVKDIVDKIRKLTNQMDEEYGCLGSLFRTGGRTTFFASQVERYADVYASSCYNLVHYPSFYYFRAPMKCMPHE-LTVDHAHTLRSRSNTLERQQSVGQQVRGWNKKTLNDQETFCHEEEEDEDQTNS 509

HSP 2 Score: 110.538 bits (275), Expect = 2.486e-124
Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   93 QQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            + S +D+ R+FVNRSL++EKI  +GFDMDYTL  YK+P  E L F++    L++ GYP+E +SFKY+  F +RGLWF+ ++G  +K D +GNIL  + G  FL
Sbjct:    5 KDSNADE-RVFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDTGYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVDGFGNILGGIHGLRFL 106
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Match: Y71H10B.1 (pep chromosome:WBcel235:X:2834222:2840292:-1 gene:WBGene00022201.1 transcript:Y71H10B.1a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H10B.1)

HSP 1 Score: 355.525 bits (911), Expect = 2.486e-124
Identity = 180/414 (43.48%), Postives = 267/414 (64.49%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDY-IVTEK-GVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLR------LNDK-NLMQGNSEDDDDTNS 1606
             L ++EI  +YPNK++   +SR+YV+NTLF++ E +L+ ++I +F  H  Y ++T+K GV+   V MSY+SI QD+R   D++H E  +K++ +E  E YV   ++ P  L +LR  G KTFLLTNS+++YT  +M++IL          +W TYF+ IVVD+ KP++F  G++ R V+ +TG   +G H GPL+ G +YSGGSC+ F K++  RGK+VLYVGDHIFGD+L+SKK  GWRT+LV+PE+ +EL VW   +  + E+  +++TL +IY  L+ AS  KP VK +  KI++L  Q++  YG LGSLFR G R TFF+SQ++RYAD+Y++SC N++HYP  Y FR+    +PHE   + H   +      L       + +R      LND+        ED+D TNS
Sbjct:  105 FLKNHEIEEMYPNKYLPLSDSRVYVLNTLFNMPETHLICQLIDFFDKHPHYSMLTDKTGVKGGEVMMSYKSIFQDLRRVIDWVHVESTMKKQVMENPEKYVIRDERAPRFLSQLREHGKKTFLLTNSDWTYTNVMMKFILG--------DDWKTYFNIIVVDSCKPRWFAEGTVFREVNTDTGSYKLGIHTGPLREGVVYSGGSCDAFQKMMKCRGKEVLYVGDHIFGDVLRSKKSRGWRTFLVVPELVNELTVWTDSKPLFDEMGHMESTLADIYKNLDGASRHKPVVKDIVDKIRKLTNQMDEEYGCLGSLFRTGGRTTFFASQVERYADVYASSCYNLVHYPSFYYFRAPMKCMPHE-LTVDHAHTLRSRSNTLERQQSVGQQVRGWNKKTLNDQETFCHEEEEDEDQTNS 509

HSP 2 Score: 110.538 bits (275), Expect = 2.486e-124
Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   93 QQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            + S +D+ R+FVNRSL++EKI  +GFDMDYTL  YK+P  E L F++    L++ GYP+E +SFKY+  F +RGLWF+ ++G  +K D +GNIL  + G  FL
Sbjct:    5 KDSNADE-RVFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDTGYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVDGFGNILGGIHGLRFL 106
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Match: Y71H10B.1 (pep chromosome:WBcel235:X:2834224:2838653:-1 gene:WBGene00022201.1 transcript:Y71H10B.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H10B.1)

HSP 1 Score: 355.525 bits (911), Expect = 2.486e-124
Identity = 180/414 (43.48%), Postives = 267/414 (64.49%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDY-IVTEK-GVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLR------LNDK-NLMQGNSEDDDDTNS 1606
             L ++EI  +YPNK++   +SR+YV+NTLF++ E +L+ ++I +F  H  Y ++T+K GV+   V MSY+SI QD+R   D++H E  +K++ +E  E YV   ++ P  L +LR  G KTFLLTNS+++YT  +M++IL          +W TYF+ IVVD+ KP++F  G++ R V+ +TG   +G H GPL+ G +YSGGSC+ F K++  RGK+VLYVGDHIFGD+L+SKK  GWRT+LV+PE+ +EL VW   +  + E+  +++TL +IY  L+ AS  KP VK +  KI++L  Q++  YG LGSLFR G R TFF+SQ++RYAD+Y++SC N++HYP  Y FR+    +PHE   + H   +      L       + +R      LND+        ED+D TNS
Sbjct:  105 FLKNHEIEEMYPNKYLPLSDSRVYVLNTLFNMPETHLICQLIDFFDKHPHYSMLTDKTGVKGGEVMMSYKSIFQDLRRVIDWVHVESTMKKQVMENPEKYVIRDERAPRFLSQLREHGKKTFLLTNSDWTYTNVMMKFILG--------DDWKTYFNIIVVDSCKPRWFAEGTVFREVNTDTGSYKLGIHTGPLREGVVYSGGSCDAFQKMMKCRGKEVLYVGDHIFGDVLRSKKSRGWRTFLVVPELVNELTVWTDSKPLFDEMGHMESTLADIYKNLDGASRHKPVVKDIVDKIRKLTNQMDEEYGCLGSLFRTGGRTTFFASQVERYADVYASSCYNLVHYPSFYYFRAPMKCMPHE-LTVDHAHTLRSRSNTLERQQSVGQQVRGWNKKTLNDQETFCHEEEEDEDQTNS 509

HSP 2 Score: 110.538 bits (275), Expect = 2.486e-124
Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   93 QQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            + S +D+ R+FVNRSL++EKI  +GFDMDYTL  YK+P  E L F++    L++ GYP+E +SFKY+  F +RGLWF+ ++G  +K D +GNIL  + G  FL
Sbjct:    5 KDSNADE-RVFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDTGYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVDGFGNILGGIHGLRFL 106
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Match: CG32549 (gene:FBgn0052549 transcript:FBtr0074570)

HSP 1 Score: 419.468 bits (1077), Expect = 8.788e-140
Identity = 192/375 (51.20%), Postives = 276/375 (73.60%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD 1486
            + GF +I   H+++  +YPNKF+K DESR+YV+NTLF+L E YLLA ++ + TN  D++  E+G++  ++  +Y+SI QDVR A D++H++G+LKR+T + + +YV   ++LP +L R+R  GAK F+LTNS+Y+YT +IM Y+   P     +   R+W TYFD IVVDA+KP FF  G++LR VD +TG L +G H+GPL+ GQ+YSGGSCE+F+K I ++GKDVLYVGDHIFGDILKSKKI GWRT+L++PE+  EL VW  E   + EL  LD  L ++Y  L+ ++  KPD+  ++  I+++  +++ +YG +GSLFR+GSR TFFSSQ+ RYAD+Y+A+ LN+I+YP  YMFR+  MLLPHES  + HD
Sbjct:  116 VHGFEFI--KHHQVYELYPNKFLKLDESRVYVLNTLFNLPETYLLACLVDFLTNSSDFVRVERGIKAGDLLFTYKSIFQDVRRAVDWVHSDGDLKRRTTQNMAHYVKKDERLPTVLSRIRESGAKLFMLTNSDYTYTNEIMTYLFDFPHGATPDEPHRDWKTYFDVIVVDARKPLFFDEGTVLRQVDTKTGSLKIGTHVGPLQPGQVYSGGSCELFTKFINAKGKDVLYVGDHIFGDILKSKKIRGWRTFLIVPELVRELHVWTDECQLFAELQNLDIKLGDLYRDLDSSAKVKPDISQLRTCIRDVTHKMDMSYGMMGSLFRSGSRQTFFSSQVVRYADVYAATLLNLIYYPFSYMFRAPAMLLPHEST-VAHD 487

HSP 2 Score: 124.405 bits (311), Expect = 1.156e-29
Identity = 56/100 (56.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL +E I++YGFDMDYTLA YKSPQ+E+L F+L ++ L+  GYP E   F+Y+  F +RGLWFD  YG  +K D YGNIL+ + GF F+
Sbjct:   23 RYAAHRVFVNRSLHLENIKYYGFDMDYTLAEYKSPQYEQLGFNLVKERLVFMGYPKEILQFEYDPTFPVRGLWFDTLYGNLLKVDAYGNILVCVHGFEFI 122
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Match: CG32549 (gene:FBgn0052549 transcript:FBtr0074575)

HSP 1 Score: 419.853 bits (1078), Expect = 1.414e-139
Identity = 192/375 (51.20%), Postives = 276/375 (73.60%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD 1486
            + GF +I   H+++  +YPNKF+K DESR+YV+NTLF+L E YLLA ++ + TN  D++  E+G++  ++  +Y+SI QDVR A D++H++G+LKR+T + + +YV   ++LP +L R+R  GAK F+LTNS+Y+YT +IM Y+   P     +   R+W TYFD IVVDA+KP FF  G++LR VD +TG L +G H+GPL+ GQ+YSGGSCE+F+K I ++GKDVLYVGDHIFGDILKSKKI GWRT+L++PE+  EL VW  E   + EL  LD  L ++Y  L+ ++  KPD+  ++  I+++  +++ +YG +GSLFR+GSR TFFSSQ+ RYAD+Y+A+ LN+I+YP  YMFR+  MLLPHES  + HD
Sbjct:  147 VHGFEFI--KHHQVYELYPNKFLKLDESRVYVLNTLFNLPETYLLACLVDFLTNSSDFVRVERGIKAGDLLFTYKSIFQDVRRAVDWVHSDGDLKRRTTQNMAHYVKKDERLPTVLSRIRESGAKLFMLTNSDYTYTNEIMTYLFDFPHGATPDEPHRDWKTYFDVIVVDARKPLFFDEGTVLRQVDTKTGSLKIGTHVGPLQPGQVYSGGSCELFTKFINAKGKDVLYVGDHIFGDILKSKKIRGWRTFLIVPELVRELHVWTDECQLFAELQNLDIKLGDLYRDLDSSAKVKPDISQLRTCIRDVTHKMDMSYGMMGSLFRSGSRQTFFSSQVVRYADVYAATLLNLIYYPFSYMFRAPAMLLPHEST-VAHD 518

HSP 2 Score: 124.405 bits (311), Expect = 1.478e-29
Identity = 56/100 (56.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL +E I++YGFDMDYTLA YKSPQ+E+L F+L ++ L+  GYP E   F+Y+  F +RGLWFD  YG  +K D YGNIL+ + GF F+
Sbjct:   54 RYAAHRVFVNRSLHLENIKYYGFDMDYTLAEYKSPQYEQLGFNLVKERLVFMGYPKEILQFEYDPTFPVRGLWFDTLYGNLLKVDAYGNILVCVHGFEFI 153
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Match: CG32549 (gene:FBgn0052549 transcript:FBtr0074574)

HSP 1 Score: 419.853 bits (1078), Expect = 1.414e-139
Identity = 192/375 (51.20%), Postives = 276/375 (73.60%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD 1486
            + GF +I   H+++  +YPNKF+K DESR+YV+NTLF+L E YLLA ++ + TN  D++  E+G++  ++  +Y+SI QDVR A D++H++G+LKR+T + + +YV   ++LP +L R+R  GAK F+LTNS+Y+YT +IM Y+   P     +   R+W TYFD IVVDA+KP FF  G++LR VD +TG L +G H+GPL+ GQ+YSGGSCE+F+K I ++GKDVLYVGDHIFGDILKSKKI GWRT+L++PE+  EL VW  E   + EL  LD  L ++Y  L+ ++  KPD+  ++  I+++  +++ +YG +GSLFR+GSR TFFSSQ+ RYAD+Y+A+ LN+I+YP  YMFR+  MLLPHES  + HD
Sbjct:  147 VHGFEFI--KHHQVYELYPNKFLKLDESRVYVLNTLFNLPETYLLACLVDFLTNSSDFVRVERGIKAGDLLFTYKSIFQDVRRAVDWVHSDGDLKRRTTQNMAHYVKKDERLPTVLSRIRESGAKLFMLTNSDYTYTNEIMTYLFDFPHGATPDEPHRDWKTYFDVIVVDARKPLFFDEGTVLRQVDTKTGSLKIGTHVGPLQPGQVYSGGSCELFTKFINAKGKDVLYVGDHIFGDILKSKKIRGWRTFLIVPELVRELHVWTDECQLFAELQNLDIKLGDLYRDLDSSAKVKPDISQLRTCIRDVTHKMDMSYGMMGSLFRSGSRQTFFSSQVVRYADVYAATLLNLIYYPFSYMFRAPAMLLPHEST-VAHD 518

HSP 2 Score: 124.405 bits (311), Expect = 1.478e-29
Identity = 56/100 (56.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL +E I++YGFDMDYTLA YKSPQ+E+L F+L ++ L+  GYP E   F+Y+  F +RGLWFD  YG  +K D YGNIL+ + GF F+
Sbjct:   54 RYAAHRVFVNRSLHLENIKYYGFDMDYTLAEYKSPQYEQLGFNLVKERLVFMGYPKEILQFEYDPTFPVRGLWFDTLYGNLLKVDAYGNILVCVHGFEFI 153
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Match: CG32549 (gene:FBgn0052549 transcript:FBtr0074571)

HSP 1 Score: 419.468 bits (1077), Expect = 3.120e-139
Identity = 192/375 (51.20%), Postives = 276/375 (73.60%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD 1486
            + GF +I   H+++  +YPNKF+K DESR+YV+NTLF+L E YLLA ++ + TN  D++  E+G++  ++  +Y+SI QDVR A D++H++G+LKR+T + + +YV   ++LP +L R+R  GAK F+LTNS+Y+YT +IM Y+   P     +   R+W TYFD IVVDA+KP FF  G++LR VD +TG L +G H+GPL+ GQ+YSGGSCE+F+K I ++GKDVLYVGDHIFGDILKSKKI GWRT+L++PE+  EL VW  E   + EL  LD  L ++Y  L+ ++  KPD+  ++  I+++  +++ +YG +GSLFR+GSR TFFSSQ+ RYAD+Y+A+ LN+I+YP  YMFR+  MLLPHES  + HD
Sbjct:  165 VHGFEFI--KHHQVYELYPNKFLKLDESRVYVLNTLFNLPETYLLACLVDFLTNSSDFVRVERGIKAGDLLFTYKSIFQDVRRAVDWVHSDGDLKRRTTQNMAHYVKKDERLPTVLSRIRESGAKLFMLTNSDYTYTNEIMTYLFDFPHGATPDEPHRDWKTYFDVIVVDARKPLFFDEGTVLRQVDTKTGSLKIGTHVGPLQPGQVYSGGSCELFTKFINAKGKDVLYVGDHIFGDILKSKKIRGWRTFLIVPELVRELHVWTDECQLFAELQNLDIKLGDLYRDLDSSAKVKPDISQLRTCIRDVTHKMDMSYGMMGSLFRSGSRQTFFSSQVVRYADVYAATLLNLIYYPFSYMFRAPAMLLPHEST-VAHD 536

HSP 2 Score: 124.405 bits (311), Expect = 1.399e-29
Identity = 55/96 (57.29%), Postives = 72/96 (75.00%), Query Frame = 3 
Query:  114 HRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            HR+FVNRSL +E I++YGFDMDYTLA YKSPQ+E+L F+L ++ L+  GYP E   F+Y+  F +RGLWFD  YG  +K D YGNIL+ + GF F+
Sbjct:   76 HRVFVNRSLHLENIKYYGFDMDYTLAEYKSPQYEQLGFNLVKERLVFMGYPKEILQFEYDPTFPVRGLWFDTLYGNLLKVDAYGNILVCVHGFEFI 171
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Match: CG32549 (gene:FBgn0052549 transcript:FBtr0340422)

HSP 1 Score: 419.853 bits (1078), Expect = 2.113e-138
Identity = 192/375 (51.20%), Postives = 276/375 (73.60%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD 1486
            + GF +I   H+++  +YPNKF+K DESR+YV+NTLF+L E YLLA ++ + TN  D++  E+G++  ++  +Y+SI QDVR A D++H++G+LKR+T + + +YV   ++LP +L R+R  GAK F+LTNS+Y+YT +IM Y+   P     +   R+W TYFD IVVDA+KP FF  G++LR VD +TG L +G H+GPL+ GQ+YSGGSCE+F+K I ++GKDVLYVGDHIFGDILKSKKI GWRT+L++PE+  EL VW  E   + EL  LD  L ++Y  L+ ++  KPD+  ++  I+++  +++ +YG +GSLFR+GSR TFFSSQ+ RYAD+Y+A+ LN+I+YP  YMFR+  MLLPHES  + HD
Sbjct:  244 VHGFEFI--KHHQVYELYPNKFLKLDESRVYVLNTLFNLPETYLLACLVDFLTNSSDFVRVERGIKAGDLLFTYKSIFQDVRRAVDWVHSDGDLKRRTTQNMAHYVKKDERLPTVLSRIRESGAKLFMLTNSDYTYTNEIMTYLFDFPHGATPDEPHRDWKTYFDVIVVDARKPLFFDEGTVLRQVDTKTGSLKIGTHVGPLQPGQVYSGGSCELFTKFINAKGKDVLYVGDHIFGDILKSKKIRGWRTFLIVPELVRELHVWTDECQLFAELQNLDIKLGDLYRDLDSSAKVKPDISQLRTCIRDVTHKMDMSYGMMGSLFRSGSRQTFFSSQVVRYADVYAATLLNLIYYPFSYMFRAPAMLLPHEST-VAHD 615

HSP 2 Score: 124.405 bits (311), Expect = 1.890e-29
Identity = 55/96 (57.29%), Postives = 72/96 (75.00%), Query Frame = 3 
Query:  114 HRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            HR+FVNRSL +E I++YGFDMDYTLA YKSPQ+E+L F+L ++ L+  GYP E   F+Y+  F +RGLWFD  YG  +K D YGNIL+ + GF F+
Sbjct:  155 HRVFVNRSLHLENIKYYGFDMDYTLAEYKSPQYEQLGFNLVKERLVFMGYPKEILQFEYDPTFPVRGLWFDTLYGNLLKVDAYGNILVCVHGFEFI 250
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Match: nt5c2a (5'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZDB-GENE-030131-3566])

HSP 1 Score: 370.163 bits (949), Expect = 3.199e-131
Identity = 184/383 (48.04%), Postives = 261/383 (68.15%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFM-KFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQ---NGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  L   EI  +YPNKF+ + D  R Y++NTLF+L E YL A ++ +FT+   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R+    AK FL+TNS+Y YT KIM Y+   P         R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + + E P
Sbjct:  124 GFNF--LRGPEIRELYPNKFIQRGDTERFYILNTLFNLPETYLFACLVDFFTSCSRYTSCEMGFKDGDLFMSFKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDAKLPLLLSRMNEV-AKVFLVTNSDYKYTDKIMTYLFDFPHGPKLGSPHRPWQSYFDLILVDARKPMFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINDTESP 503

HSP 2 Score: 119.783 bits (299), Expect = 3.199e-131
Identity = 58/100 (58.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF FL
Sbjct:   29 REVHHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPAFPTRGLVFDTTYGNLLKVDAYGNILVCAHGFNFL 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Match: nt5c2a (5'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZDB-GENE-030131-3566])

HSP 1 Score: 370.163 bits (949), Expect = 3.199e-131
Identity = 184/383 (48.04%), Postives = 261/383 (68.15%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFM-KFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQ---NGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  L   EI  +YPNKF+ + D  R Y++NTLF+L E YL A ++ +FT+   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R+    AK FL+TNS+Y YT KIM Y+   P         R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + + E P
Sbjct:  124 GFNF--LRGPEIRELYPNKFIQRGDTERFYILNTLFNLPETYLFACLVDFFTSCSRYTSCEMGFKDGDLFMSFKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDAKLPLLLSRMNEV-AKVFLVTNSDYKYTDKIMTYLFDFPHGPKLGSPHRPWQSYFDLILVDARKPMFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINDTESP 503

HSP 2 Score: 119.783 bits (299), Expect = 3.199e-131
Identity = 58/100 (58.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF FL
Sbjct:   29 REVHHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPAFPTRGLVFDTTYGNLLKVDAYGNILVCAHGFNFL 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Match: nt5c2a (5'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZDB-GENE-030131-3566])

HSP 1 Score: 360.533 bits (924), Expect = 2.322e-128
Identity = 183/383 (47.78%), Postives = 258/383 (67.36%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFM-KFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQ---NGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  L   EI  +YPNKF+ + D  R Y++NTLF+L E YL A ++ +FT+   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R+    AK FL+TNS+Y YT KIM Y+   P         R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL          HEL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + + E P
Sbjct:  124 GFNF--LRGPEIRELYPNKFIQRGDTERFYILNTLFNLPETYLFACLVDFFTSCSRYTSCEMGFKDGDLFMSFKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDAKLPLLLSRMNEV-AKVFLVTNSDYKYTDKIMTYLFDFPHGPKLGSPHRPWQSYFDLILVDARKPMFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQEL----------HELQSLDIFLAELYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINDTESP 493

HSP 2 Score: 119.783 bits (299), Expect = 2.322e-128
Identity = 58/100 (58.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF FL
Sbjct:   29 REVHHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPAFPTRGLVFDTTYGNLLKVDAYGNILVCAHGFNFL 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Match: nt5c2l1 (5'-nucleotidase, cytosolic II, like 1 [Source:ZFIN;Acc:ZDB-GENE-031006-8])

HSP 1 Score: 343.584 bits (880), Expect = 1.885e-121
Identity = 175/408 (42.89%), Postives = 262/408 (64.22%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYIL-SVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDD 1594
            GF +  L   ++   YPNKF++ D++ R YV+NTLF+LSE YL A ++ ++T    Y    KG +  ++FMS++S+ QDVRDA D++H  G LK +TL+ L+ YV    ++P +L R++   AK FL TNS+++YT  IM+Y+L +     G  + W +YFD +VVD +KP FF  G++LR VD +TGKL +G + G L+ G +YSGGS ++   L+  +GKD+LYVGDHIFGDILKSKK  GW+T+LV+PE+  EL VW  +   + EL  LD  L  +Y  L+  S + PD+  ++ +IK +   ++  YG +GSL R GS  T F+SQ+ RYAD+YS +CLN+++YP  ++FR+  +LLPHE+A+ T +   +++  +L       EH+R       Q   ED D
Sbjct:  103 GFEY--LRGEQVDEYYPNKFIQRDDTDRFYVLNTLFNLSETYLYACLVDFYTKSTRYKNWPKGFKHGDLFMSHRSMFQDVRDAMDHLHDTGTLKERTLKNLDKYVIKDPRIPVLLSRIKEV-AKVFLATNSDFNYTEAIMKYLLDAATSSKGMKKSWRSYFDLVVVDTRKPLFFAGGTVLRQVDTDTGKLRIGTYTGDLQHGTVYSGGSSDLVCDLLDVKGKDILYVGDHIFGDILKSKKRQGWKTFLVVPELVKELHVWADKSSMFEELKHLDIFLAELYQHLDSGSQECPDISAIQTRIKMVTYGMDLCYGKMGSLLRCGSTKTLFASQLLRYADIYSTTCLNLLNYPFSFLFRAPLVLLPHEAAVETQN---KEQMAEL------SEHMRRTSLKQRQCECEDGD 498

HSP 2 Score: 113.62 bits (283), Expect = 1.885e-121
Identity = 53/97 (54.64%), Postives = 67/97 (69.07%), Query Frame = 3 
Query:  111 KHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            +HR+FVNRSL +E I+ YGFDMDYTLA YKSP++E L F+L RD L+  GYP E   + Y+S F  RGL +D  YG  +K D  GNIL+   GF +L
Sbjct:   11 QHRVFVNRSLTLENIKCYGFDMDYTLAVYKSPEYESLGFELLRDRLVSIGYPHELLGYTYDSTFPTRGLVYDTTYGNLLKIDSNGNILVCTHGFEYL 107
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Match: nt5c2b (5'-nucleotidase, cytosolic IIb [Source:ZFIN;Acc:ZDB-GENE-061013-522])

HSP 1 Score: 369.392 bits (947), Expect = 1.376e-120
Identity = 179/372 (48.12%), Postives = 260/372 (69.89%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP--DQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            + GFN+  L   +I  +YPNKF++ D++ R Y++NTLF+L E YLLA ++ +F+N   Y   E G +  ++FMS++S+ QDVRDA D++H +G LK KT+E LE YV    KLP +L R+    +K FL TNS+Y YT KIM Y+   P   ++G   R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ ++K++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +
Sbjct:  123 VHGFNF--LRGPDIRELYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFSNCDRYSSCETGFKDGDLFMSFKSMFQDVRDAVDWVHFKGTLKEKTVENLEKYVVKDAKLPLLLSRMNEV-SKVFLATNSDYKYTHKIMTYLFDFPHGPKHGTPHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQGLDIFLAELYKHLDSSSNERPDISAIQRRMKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV 491

HSP 2 Score: 119.013 bits (297), Expect = 8.705e-28
Identity = 58/100 (58.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E   F Y+  F  RGL FD  YG  +K D YGNIL+ + GF FL
Sbjct:   30 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLGFVYDPTFPTRGLVFDNMYGNLLKVDAYGNILVCVHGFNFL 129
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Match: nt5c2 (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:548490])

HSP 1 Score: 376.326 bits (965), Expect = 1.340e-132
Identity = 187/383 (48.83%), Postives = 264/383 (68.93%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E L  YV    KLP +L R+   G K FL+TNS+Y YT KIM Y+  +P       + R W TYFD I+VDA+KP FF  G++LR VD  TGKL +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  194 GFNF--MRGPEIREQYPNKFIQRDDTDRFYILNTLFNLPETYLLACLVDFFTNCDRYTSCEMGFKDGDLFMSFRSMFQDVRDAVDWVHYKGSLKEKTVENLPKYVVKDPKLPLLLSRMNEVG-KVFLVTNSDYKYTHKIMTYLFDLPHGPKPGSSHRLWQTYFDLILVDARKPLFFGEGTVLRQVDTNTGKLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDIHETESP 573

HSP 2 Score: 119.013 bits (297), Expect = 1.340e-132
Identity = 56/96 (58.33%), Postives = 68/96 (70.83%), Query Frame = 3 
Query:  114 HRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:  103 HRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFM 198
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Match: nt5c2 (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:548490])

HSP 1 Score: 370.163 bits (949), Expect = 1.157e-130
Identity = 187/398 (46.98%), Postives = 264/398 (66.33%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP------------------DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E L  YV    KLP +L R+   G K FL+TNS+Y YT KIM Y+  +P                      + R W TYFD I+VDA+KP FF  G++LR VD  TGKL +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  139 GFNF--MRGPEIREQYPNKFIQRDDTDRFYILNTLFNLPETYLLACLVDFFTNCDRYTSCEMGFKDGDLFMSFRSMFQDVRDAVDWVHYKGSLKEKTVENLPKYVVKDPKLPLLLSRMNEVG-KVFLVTNSDYKYTHKIMTYLFDLPHGPKKENLQMLAGRRGIAKPGSSHRLWQTYFDLILVDARKPLFFGEGTVLRQVDTNTGKLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDIHETESP 533

HSP 2 Score: 118.627 bits (296), Expect = 1.157e-130
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   44 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFM 143
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Match: tti1 (TELO2 interacting protein 1 [Source:Xenbase;Acc:XB-GENE-5868172])

HSP 1 Score: 348.977 bits (894), Expect = 2.291e-122
Identity = 181/412 (43.93%), Postives = 270/412 (65.53%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDESR-IYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYIL---SVPDQNGNI--REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDD 1594
            GF++  L   EI   YPNK ++ D++R  +V+NTLF+LSE YL A ++ +FTN   Y+  + G +  N+FMS++SI QDVRDA D++H  G LK KTL+ LE YV    + P +L R+R  G K FL TNS+Y+YT  IM Y+      PD++ +   + W +YFD IVVD +KP FF  G++LR V+ + GKL +G + GP +   +YSGGS +I   L+G +GK++LYVGDHIFGDILKSKK  GWRT+LV+PE+  EL VW  +   + EL  LD  L + Y  ++ +S++ PD+  ++ +I+ +  +++  +G +GSLFR GSR T FSSQ+ RYAD+Y+AS +N+++YP  Y+F++ P+L+PHES +    +  E E  + +YF     + +   K L   +SE+D+
Sbjct:  132 GFHF--LKGPEIWKFYPNKMIQRDDTRRFHVLNTLFNLSETYLYACLVDFFTNCSRYLTCDTGYRHGNLFMSFRSIHQDVRDAMDFMHDSGCLKEKTLKDLEKYVIKDPRTPVLLTRMREVG-KVFLATNSDYNYTDAIMTYLFDDGQDPDKDMDTPKKSWRSYFDLIVVDTRKPLFFAEGTVLRQVNTDNGKLRIGTYTGPHQHCAVYSGGSSDIVCDLLGVKGKEILYVGDHIFGDILKSKKKQGWRTFLVVPELAKELTVWAHKSELFDELKNLDVVLAHSYQHMDSSSNKPPDISAIQKQIQRVTHEMDMCHGKMGSLFRCGSRQTLFSSQLIRYADIYAASFVNLMYYPFSYLFQAHPVLMPHESTVEQVSADGEGESKEQLYF-----YGKQRLKQLTHDSSEEDN 535

HSP 2 Score: 111.694 bits (278), Expect = 2.291e-122
Identity = 54/96 (56.25%), Postives = 65/96 (67.71%), Query Frame = 3 
Query:  114 HRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            HR+FVNRSL +EKI+ YGFDMDYTLA Y SP  EEL F+L  D L+  GYP E   +KY+  F  RGL FD  +G  +K D  GNIL+   GF+FL
Sbjct:   41 HRVFVNRSLALEKIKCYGFDMDYTLAMYSSPAHEELGFELLLDRLVSIGYPHEILEYKYDPSFPTRGLVFDNLFGNLLKVDSNGNILVCTHGFHFL 136
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Match: nt5c2 (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:548490])

HSP 1 Score: 342.428 bits (877), Expect = 2.510e-122
Identity = 177/383 (46.21%), Postives = 250/383 (65.27%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E L  YV    KLP +L R+   G K FL+TNS+Y YT KIM Y+  +P       + R W TYFD I+VDA+KP FF  G++LR VD  TGKL +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE                               L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  130 GFNF--MRGPEIREQYPNKFIQRDDTDRFYILNTLFNLPETYLLACLVDFFTNCDRYTSCEMGFKDGDLFMSFRSMFQDVRDAVDWVHYKGSLKEKTVENLPKYVVKDPKLPLLLSRMNEVG-KVFLVTNSDYKYTHKIMTYLFDLPHGPKPGSSHRLWQTYFDLILVDARKPLFFGEGTVLRQVDTNTGKLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPE------------------------------HLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDIHETESP 479

HSP 2 Score: 118.242 bits (295), Expect = 2.510e-122
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   35 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFM 134
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Match: nt5c2 (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:548490])

HSP 1 Score: 342.428 bits (877), Expect = 2.727e-122
Identity = 177/383 (46.21%), Postives = 250/383 (65.27%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E L  YV    KLP +L R+   G K FL+TNS+Y YT KIM Y+  +P       + R W TYFD I+VDA+KP FF  G++LR VD  TGKL +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE                               L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  150 GFNF--MRGPEIREQYPNKFIQRDDTDRFYILNTLFNLPETYLLACLVDFFTNCDRYTSCEMGFKDGDLFMSFRSMFQDVRDAVDWVHYKGSLKEKTVENLPKYVVKDPKLPLLLSRMNEVG-KVFLVTNSDYKYTHKIMTYLFDLPHGPKPGSSHRLWQTYFDLILVDARKPLFFGEGTVLRQVDTNTGKLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPE------------------------------HLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDIHETESP 499

HSP 2 Score: 118.627 bits (296), Expect = 2.727e-122
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   55 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFM 154
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Match: Nt5c2 (5'-nucleotidase, cytosolic II [Source:MGI Symbol;Acc:MGI:2178563])

HSP 1 Score: 374.785 bits (961), Expect = 4.523e-132
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  149 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 528

HSP 2 Score: 118.242 bits (295), Expect = 4.523e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   54 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 153
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Match: Nt5c2 (5'-nucleotidase, cytosolic II [Source:MGI Symbol;Acc:MGI:2178563])

HSP 1 Score: 374.785 bits (961), Expect = 4.957e-132
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 4.957e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Match: Nt5c2 (5'-nucleotidase, cytosolic II [Source:MGI Symbol;Acc:MGI:2178563])

HSP 1 Score: 374.785 bits (961), Expect = 4.957e-132
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 4.957e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Match: Nt5c2 (5'-nucleotidase, cytosolic II [Source:MGI Symbol;Acc:MGI:2178563])

HSP 1 Score: 374.4 bits (960), Expect = 5.082e-132
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  150 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 529

HSP 2 Score: 117.857 bits (294), Expect = 5.082e-132
Identity = 56/96 (58.33%), Postives = 69/96 (71.88%), Query Frame = 3 
Query:  114 HRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   59 HRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 154
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Match: Nt5c2 (5'-nucleotidase, cytosolic II [Source:MGI Symbol;Acc:MGI:2178563])

HSP 1 Score: 374.4 bits (960), Expect = 5.962e-131
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:   92 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 471

HSP 2 Score: 114.775 bits (286), Expect = 5.962e-131
Identity = 55/95 (57.89%), Postives = 68/95 (71.58%), Query Frame = 3 
Query:  117 RIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:    2 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 96
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Match: sp|Q5RA22|5NTC_PONAB (Cytosolic purine 5'-nucleotidase OS=Pongo abelii OX=9601 GN=NT5C2 PE=2 SV=1)

HSP 1 Score: 376.326 bits (965), Expect = 9.286e-132
Identity = 187/383 (48.83%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 9.286e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Match: sp|P49902|5NTC_HUMAN (Cytosolic purine 5'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5C2 PE=1 SV=1)

HSP 1 Score: 376.326 bits (965), Expect = 9.286e-132
Identity = 187/383 (48.83%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 9.286e-132
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Match: sp|Q5EBF1|5NTC_XENTR (Cytosolic purine 5'-nucleotidase OS=Xenopus tropicalis OX=8364 GN=nt5c2 PE=2 SV=1)

HSP 1 Score: 375.941 bits (964), Expect = 1.061e-131
Identity = 187/383 (48.83%), Postives = 264/383 (68.93%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E L  YV    KLP +L R+   G K FL+TNS+Y YT KIM Y+  +P       + R W TYFD I+VDA+KP FF  G++LR VD  TGKL +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNF--MRGPEIREQYPNKFIQRDDTDRFYILNTLFNLPETYLLACLVDFFTNCDRYTSCEMGFKDGDLFMSFRSMFQDVRDAVDWVHYKGSLKEKTVENLPKYVVKDPKLPLLLSRMNEVG-KVFLVTNSDYKYTHKIMTYLFDLPHGPKPGSSHRLWQTYFDLILVDARKPLFFGEGTVLRQVDTNTGKLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDIHETESP 503

HSP 2 Score: 118.242 bits (295), Expect = 1.061e-131
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFM 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Match: sp|O46411|5NTC_BOVIN (Cytosolic purine 5'-nucleotidase OS=Bos taurus OX=9913 GN=NT5C2 PE=1 SV=1)

HSP 1 Score: 375.941 bits (964), Expect = 1.222e-131
Identity = 187/383 (48.83%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 1.222e-131
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Match: sp|Q3V1L4|5NTC_MOUSE (Cytosolic purine 5'-nucleotidase OS=Mus musculus OX=10090 GN=Nt5c2 PE=1 SV=2)

HSP 1 Score: 374.785 bits (961), Expect = 3.000e-131
Identity = 186/383 (48.56%), Postives = 263/383 (68.67%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+I     E    YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   + G +  ++FMSY+S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R++  G K FL TNS+Y YT KIM Y+   P       + R W +YFD I+VDA+KP FF  G++LR VD +TGKL +G + GPL+ G +YSGGS +    L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +  TH  + E E P
Sbjct:  124 GFNFI--RGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVG-KVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESP 503

HSP 2 Score: 117.857 bits (294), Expect = 3.000e-131
Identity = 57/100 (57.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+S F  RGL FD  YG  +K D YGN+L+   GF F+
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFI 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Match: A0A3Q0KP95 (Putative cytosolic purine 5-nucleotidase OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 465.307 bits (1196), Expect = 9.985e-155
Identity = 225/412 (54.61%), Postives = 299/412 (72.57%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNS 1606
            + GFN+I     E+  +YPNKF+K+DE RI +MNTLFSL E Y+L+ +IH F N  DY   E GV+K +++MSY SI +DVR A D++H  G LK++TL  +  YV    +L  +LDRLR  GAK FLLTNS++ Y+  IM YIL  P  +G IR+WT+YFDYIV DA+KP FF  G+ILR V ++TG+  +G H+GPL+ GQIYSGGSCE+FS LIG+RGKDVLYVGDH++GDILKSKK VGWRTYL+IPE+ +E++VW+K++  + EL K+DN LE +Y  LNI+S+ +PDV  ++ KI+ +A Q+E +YG LGS+FRNGSR TFFSSQ+ RYADLYS SC+N+I+YPLCYMFR+  ML+PHES  ++H    +D + DL      P  LR   +N +   S D +   S
Sbjct:  113 LRGFNFI--QREELRSMYPNKFVKYDEKRIEIMNTLFSLPETYILSCIIHMFVNDPDYTKVEHGVKKGSLYMSYASIYEDVRTACDWMH-RGELKQRTLANISEYVERDPRLCTLLDRLRVNGAKVFLLTNSDFEYSHAIMSYILGDPRPDGTIRKWTSYFDYIVTDARKPAFFQEGTILRVVCQDTGQPSIGHHMGPLETGQIYSGGSCEVFSNLIGARGKDVLYVGDHVYGDILKSKKTVGWRTYLIIPELANEIYVWKKKKSLFDELQKIDNNLETVYRDLNISSNIRPDVGDLQRKIRAVAQQMEESYGALGSIFRNGSRHTFFSSQVLRYADLYSFSCINLIYYPLCYMFRAPAMLMPHEST-VSHGDSPKDSFSDLDAL---PCGLRRRTRNEITPLSPDKNGLKS 517

HSP 2 Score: 132.109 bits (331), Expect = 6.976e-29
Identity = 62/110 (56.36%), Postives = 79/110 (71.82%), Query Frame = 3 
Query:   72 KSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            +S+       R  KHRIFVNRSLQ++KI+F+GFDMDYTLA YKSPQ+EEL F++ +  LI+ GYP +   F Y+  F LRGLWFD+ YG  +K DQ+GNIL  L GF F+
Sbjct:   10 ESSPSIRVHKRETKHRIFVNRSLQLDKIKFFGFDMDYTLAQYKSPQYEELAFNIIKSRLIKLGYPPKTSDFVYDPSFPLRGLWFDRLYGTLLKMDQFGNILSCLRGFNFI 119
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Match: A0A4Z2D6P9 (Cytosolic purine 5'-nucleotidase isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_004047 PE=4 SV=1)

HSP 1 Score: 456.447 bits (1173), Expect = 6.319e-152
Identity = 218/412 (52.91%), Postives = 300/412 (72.82%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNS 1606
            + GF++I     E+  +YPNKF+K+DE RI +MNTLFSL E Y+L+ +I  F +  DY   E G++K +++MSY SI +DVR A D++H  G LK++TL  +  YV    +L  +LDRLR  G+K FLLTNS + Y+  IM +IL +P  +G +R+WT+YFDYIV DA+KP FF  G+ILRAV ++TG+  +G H+GPL+ GQIYSGGSCE+FS LIG+RGKDVLYVGDH++GDILKSKK VGWRTYL+IPE+ +E++VW+K++  + EL ++DN LE IY  LNI+S+ +PDV  ++ KI+ +A Q+E +YG LGS+FRNGSR TFFSSQ+ RYADLYS SC+N+I+YPLCYMFR+  ML+PHES  ++H    +D + D+      P  LR   +N +   + D  D +S
Sbjct:   67 LRGFSFI--QREELRAMYPNKFVKYDEKRIEIMNTLFSLPETYMLSCIISMFISDPDYTKVEHGLKKGSLYMSYASIYEDVRTACDWMH-RGELKQRTLANIGEYVERDPRLCVLLDRLRVNGSKVFLLTNSEFEYSHGIMSFILEIPKPDGTVRKWTSYFDYIVTDARKPAFFQEGTILRAVCQDTGQPSIGHHMGPLETGQIYSGGSCEVFSNLIGARGKDVLYVGDHVYGDILKSKKTVGWRTYLIIPELANEIYVWKKKKSLFDELQRIDNNLETIYRDLNISSNIRPDVGDLQRKIRAVAQQMEESYGLLGSIFRNGSRHTFFSSQVLRYADLYSFSCINLIYYPLCYMFRAPAMLMPHEST-VSHGDSPKDSFSDMDAL---PCSLRRRTRNGIASLTSDKSDLSS 471

HSP 2 Score: 86.6557 bits (213), Expect = 3.946e-14
Identity = 39/69 (56.52%), Postives = 49/69 (71.01%), Query Frame = 3 
Query:  195 YKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            YKSPQ+EEL FD+ +  LI+ GYP +  SF Y+  F LRGLWFD+ YG  +K DQ+GNIL  L GF F+
Sbjct:    5 YKSPQYEELAFDIIKTRLIKLGYPSKLSSFTYDPSFPLRGLWFDRLYGTLLKMDQFGNILACLRGFSFI 73
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Match: A0A1S3I3S5 (cytosolic purine 5'-nucleotidase isoform X3 OS=Lingula unguis OX=7574 GN=LOC106160766 PE=4 SV=1)

HSP 1 Score: 427.557 bits (1098), Expect = 1.122e-151
Identity = 200/399 (50.13%), Postives = 285/399 (71.43%), Query Frame = 2 
Query:  392 LLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSE 1585
             L  +E++  YPNKF++FD+ R+Y++NTLF+L E YLLA ++ +FTN + Y   + GV+  ++FMSY+SI QDVR A D++H +G+LK  T+E L+ YV    +L  MLDR+RS GA TFLLTNS+Y YT  +M Y+L   + +G + R+WTTYFDYIVVDA+KP FF  G+ LR VD++TG   +G H+GPL+  Q+YSGGSC++FS +IG++G+DVLY+GDHIFGDILKSKKI GWRT+L +PE++ ELFVW  +   Y +L  LD TL ++Y  L+ ++ Q PD++ +   I++L  +++ +YG +GSLFR+GSR TFF+SQ+ RYADLY+A+ LN++ YP  YMF++ PML+PHE A + HD   +D          SP   R    ++ QG +E
Sbjct:  137 FLKPHEVMKYYPNKFIQFDDKRLYILNTLFNLPETYLLACMVDYFTNAEGYTGEDTGVKYGDLFMSYKSIFQDVRGAVDWVHIKGSLKSTTVENLDEYVVKDPRLSVMLDRMRSHGAATFLLTNSDYEYTNGVMSYLLDRKNNDGEVVRDWTTYFDYIVVDARKPLFFSEGTTLRQVDRKTGSYSIGTHMGPLRKHQVYSGGSCDVFSDMIGAKGRDVLYIGDHIFGDILKSKKIRGWRTFLQVPELSQELFVWTDKHEMYEQLQALDATLASVYKDLDSSATQAPDIRKINKSIRDLVHKMDMSYGMMGSLFRSGSRQTFFASQVIRYADLYAANFLNLLFYPFSYMFKAPPMLMPHE-ATVKHDEDSKD------ILEESPSAARGRHYSVDQGTTE 528

HSP 2 Score: 140.969 bits (354), Expect = 1.122e-151
Identity = 62/106 (58.49%), Postives = 81/106 (76.42%), Query Frame = 3 
Query:   84 ETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            + LQ  R  +HR+FVNRSL +EKI+FYGFDMDYTLA+YKSPQ+E + F+L    L++ GYP E + F+Y+  F +RGLWFDK YG  +K DQ+GNIL+ + GFYFL
Sbjct:   33 DPLQFKRETRHRVFVNRSLHLEKIKFYGFDMDYTLADYKSPQYESMAFNLVVQRLVDIGYPSEIKEFQYQPTFPVRGLWFDKLYGNLLKVDQFGNILVCVHGFYFL 138
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Match: Q5DBL4 (SJCHGC02464 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 453.366 bits (1165), Expect = 4.924e-151
Identity = 212/382 (55.50%), Postives = 289/382 (75.65%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDL 1516
            + GF++I     E+  +YPNKF+K+DE RI +MNTLFSL E Y+L+ +I  F +  DY   E G++K +++MSY SI +DVR A D++H  G LK++TL  +  YV    +L  +LDRLR  G+K FLLTNS + Y+  IM +IL +P  +G +R+WT+YFDYIV DA+KP FF  G+ILRAV ++TG+  +G H+GPL+ GQIYSGGSCE+FS LIG+RGKDVLYVGDH++GDILKSKK VGWRTYL+IPE+ +E++VW+K++  + EL ++DN LE IY  LNI+S+ +PDV  ++ KI+ +A Q+E +YG LGS+FRNGSR TFFSSQ+ RYADLYS SC+N+I+YPLCYMFR+  ML+PHES  ++H    +D + DL
Sbjct:   70 LRGFSFI--QREELRAMYPNKFVKYDEKRIEIMNTLFSLPETYMLSCIISMFISDPDYTKVEHGLKKGSLYMSYASIYEDVRTACDWMH-RGELKQRTLANIGEYVERDPRLCVLLDRLRVNGSKVFLLTNSEFEYSHGIMSFILEIPKPDGTVRKWTSYFDYIVTDARKPAFFQEGTILRAVCQDTGQPSIGHHMGPLETGQIYSGGSCEVFSNLIGARGKDVLYVGDHVYGDILKSKKTVGWRTYLIIPELANEIYVWKKKKSLFDELQRIDNNLETIYRDLNISSNIRPDVGDLQRKIRAVAQQMEESYGLLGSIFRNGSRHTFFSSQVLRYADLYSFSCINLIYYPLCYMFRAPAMLMPHEST-VSHGDSPKDSFSDL 447

HSP 2 Score: 98.5969 bits (244), Expect = 4.846e-18
Identity = 45/76 (59.21%), Postives = 55/76 (72.37%), Query Frame = 3 
Query:  174 MDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            MDYTLA YKSPQ+EEL FD+ +  LI+ GYP +  SF Y+  F LRGLWFD+ YG  +K DQ+GNIL  L GF F+
Sbjct:    1 MDYTLAQYKSPQYEELAFDIIKTRLIKLGYPSKLSSFTYDPSFPLRGLWFDRLYGTLLKMDQFGNILACLRGFSFI 76
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Match: Q2LE75 (Cytosolic 5'-nucleotidase II (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 453.751 bits (1166), Expect = 1.287e-150
Identity = 212/382 (55.50%), Postives = 289/382 (75.65%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDL 1516
            + GF++I     E+  +YPNKF+K+DE RI +MNTLFSL E Y+L+ +I  F +  DY   E G++K +++MSY SI +DVR A D++H  G LK++TL  +  YV    +L  +LDRLR  G+K FLLTNS + Y+  IM +IL +P  +G +R+WT+YFDYIV DA+KP FF  G+ILRAV ++TG+  +G H+GPL+ GQIYSGGSCE+FS LIG+RGKDVLYVGDH++GDILKSKK VGWRTYL+IPE+ +E++VW+K++  + EL ++DN LE IY  LNI+S+ +PDV  ++ KI+ +A Q+E +YG LGS+FRNGSR TFFSSQ+ RYADLYS SC+N+I+YPLCYMFR+  ML+PHES  ++H    +D + DL
Sbjct:  108 LRGFSFI--QREELRAMYPNKFVKYDEKRIEIMNTLFSLPETYMLSCIISMFISDPDYTKVEHGLKKGSLYMSYASIYEDVRTACDWMH-RGELKQRTLANIGEYVERDPRLCVLLDRLRVNGSKVFLLTNSEFEYSHGIMSFILEIPKPDGTVRKWTSYFDYIVTDARKPAFFQEGTILRAVCQDTGQPSIGHHMGPLETGQIYSGGSCEVFSNLIGARGKDVLYVGDHVYGDILKSKKTVGWRTYLIIPELANEIYVWKKKKSLFDELQRIDNNLETIYRDLNISSNIRPDVGDLQRKIRAVAQQMEESYGLLGSIFRNGSRHTFFSSQVLRYADLYSFSCINLIYYPLCYMFRAPAMLMPHEST-VSHGDSPKDSFSDL 485

HSP 2 Score: 137.887 bits (346), Expect = 5.589e-31
Identity = 63/102 (61.76%), Postives = 77/102 (75.49%), Query Frame = 3 
Query:   96 QSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
              R  KHRIFVNRSLQ++KI+F+GFDMDYTLA YKSPQ+EEL FD+ +  LI+ GYP +  SF Y+  F LRGLWFD+ YG  +K DQ+GNIL  L GF F+
Sbjct:   13 HKRETKHRIFVNRSLQLDKIKFFGFDMDYTLAQYKSPQYEELAFDIIKTRLIKLGYPSKLSSFTYDPSFPLRGLWFDRLYGTLLKMDQFGNILACLRGFSFI 114
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Match: nt5c2a (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:103037283])

HSP 1 Score: 364.385 bits (934), Expect = 4.407e-129
Identity = 181/383 (47.26%), Postives = 258/383 (67.36%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFM-KFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI  +YPNKF+ + D  R Y++NTLF+L E YL A ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R+    AK FL TNS+  YT KIM Y+   P         R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+A+ +N+++YP  Y+FR+  + +PHES +  TH  + + E P
Sbjct:  126 GFNF--MRGPEIRELYPNKFIQRGDTERFYILNTLFNLPETYLFACLVDFFTNCSRYTSCETGFKDGDLFMSFKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDVKLPLLLSRMNEV-AKVFLATNSDCKYTDKIMTYLFDFPHGPTAGSPHRPWQSYFDLILVDARKPVFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAATFINLLYYPFSYLFRAAHVQMPHESTVEHTHVDINDTESP 505

HSP 2 Score: 118.242 bits (295), Expect = 4.407e-129
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   31 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPSFPTRGLVFDTTYGNLLKVDAYGNILVCAHGFNFM 130
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Match: nt5c2a (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:103037283])

HSP 1 Score: 364.385 bits (934), Expect = 3.216e-128
Identity = 181/383 (47.26%), Postives = 258/383 (67.36%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFM-KFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYP 1510
            GFN+  +   EI  +YPNKF+ + D  R Y++NTLF+L E YL A ++ +FTN   Y   E G +  ++FMS++S+ QDVRDA D++H +G+LK KT+E LE YV    KLP +L R+    AK FL TNS+  YT KIM Y+   P         R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+A+ +N+++YP  Y+FR+  + +PHES +  TH  + + E P
Sbjct:  139 GFNF--MRGPEIRELYPNKFIQRGDTERFYILNTLFNLPETYLFACLVDFFTNCSRYTSCETGFKDGDLFMSFKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDVKLPLLLSRMNEV-AKVFLATNSDCKYTDKIMTYLFDFPHGPTAGSPHRPWQSYFDLILVDARKPVFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAATFINLLYYPFSYLFRAAHVQMPHESTVEHTHVDINDTESP 518

HSP 2 Score: 115.161 bits (287), Expect = 3.216e-128
Identity = 55/95 (57.89%), Postives = 67/95 (70.53%), Query Frame = 3 
Query:  117 RIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDLT + L+  GYP E  +F Y+  F  RGL FD  YG  +K D YGNIL+   GF F+
Sbjct:   49 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPSFPTRGLVFDTTYGNLLKVDAYGNILVCAHGFNFM 143
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Match: nt5c2b (cytosolic purine 5'-nucleotidase-like [Source:NCBI gene;Acc:103025310])

HSP 1 Score: 368.237 bits (944), Expect = 4.024e-120
Identity = 177/372 (47.58%), Postives = 260/372 (69.89%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP--DQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            + GFN+  L   +I  +YPNKF++ D++ R Y++NTLF+L E YL A ++ +F+N   Y   E G +  ++FMS++S+ QDVRDA D++H +G LK KT+E LE YV    KLP +L R+    +K FL TNS+Y YT KIM Y+   P   ++G+  R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +I   L+G++GKD++Y+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ ++K++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +
Sbjct:  122 VHGFNF--LRGPDIRELYPNKFIQRDDTERFYILNTLFNLPETYLYACLVDFFSNCDRYASCETGFKNGDLFMSFKSMFQDVRDAVDWVHFKGTLKEKTVENLEKYVVKDPKLPLLLSRMNEV-SKVFLATNSDYKYTDKIMTYLFDFPYGPKHGSPHRPWQSYFDLILVDARKPLFFAEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDIVCDLLGAKGKDIIYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQGLDIFLAELYKHLDSSSNERPDISAIQRRVKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV 490

HSP 2 Score: 116.316 bits (290), Expect = 5.393e-27
Identity = 57/100 (57.00%), Postives = 68/100 (68.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HR+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L FDL  + L+  GYP E   F Y+  F  RGL FD  YG  +K D YGNIL+ + GF FL
Sbjct:   29 REAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLIVERLVSIGYPQELLGFVYDPSFPTRGLVFDTMYGNLLKVDAYGNILVCVHGFNFL 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Match: nt5c2l1 (cytosolic purine 5'-nucleotidase-like [Source:NCBI gene;Acc:103041485])

HSP 1 Score: 359.762 bits (922), Expect = 2.767e-117
Identity = 184/415 (44.34%), Postives = 274/415 (66.02%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIRE-WTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLI---YFSRSPEHLRLND------KNLMQGNS 1582
            +    +I L+  EI+H YP +F++ D++ R Y++NTLF+LSE YL A ++ +FT    Y   ++G +  ++FMSY+S+ QDVRDA +Y+H  G+LK +TL+ LE Y+    ++P ML R+++  AK FL TNS+Y+YT  IM+Y+L  P  + N ++ W +YFD +VVD +KP FF  G++LR VD  TGKL +G + G L+ G +YSGGS ++   L+  RGK++LYVGDHIFGDILKSKK  GW+T+LV+PE+  EL VW ++ G + EL +LD  L  ++  L+      PD+ +++ KIK++   ++  YG +GSL R G   T F+SQ+ RYADLY+ASCLN+I+YPL Y+F +  +L+PHESA+  H S  +   PDL    +F+ + + L   D       N  QGN+
Sbjct:  110 VCAHGFIYLTGAEILHYYPTRFIQRDDTERFYILNTLFNLSETYLYACLVDFFTGCSRYKNCQQGFKHGDLFMSYKSMFQDVRDAMNYLHDTGSLKERTLKNLEKYIIRDPRVPEMLSRIKNV-AKVFLATNSDYNYTEGIMKYLLESPPGSKNPKKLWRSYFDLVVVDTRKPLFFAEGTVLRQVDTNTGKLRIGTYTGDLQHGTVYSGGSSDLVCDLLDVRGKEILYVGDHIFGDILKSKKRQGWKTFLVVPELAKELQVWTEKNGMFQELRQLDVYLSELHKYLDSGIVGSPDISLIQSKIKKVTYSMDLCYGKMGSLLRYGCTKTLFASQLLRYADLYAASCLNLINYPLTYLFSASSVLMPHESAVSPHLSQ-DPPIPDLSANNFFTPTLKMLCQCDGDNCGQDNCAQGNT 522

HSP 2 Score: 117.472 bits (293), Expect = 1.696e-27
Identity = 53/110 (48.18%), Postives = 72/110 (65.45%), Query Frame = 3 
Query:   75 SNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLT 404
            + +E L   R   +R+FVN+ + +E I+ YGFDMDYTLA YKSP++E LCF+L RD L+  GYP E   + Y+  F  RGL +D  YG  +K D +GNIL+   GF +LT
Sbjct:   10 TAAEALAGKRDIHNRVFVNKGVTLENIKCYGFDMDYTLAIYKSPEYEGLCFELLRDRLVSVGYPHELLRYSYDPNFPTRGLVYDSSYGNLLKVDSHGNILVCAHGFIYLT 119
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Match: nt5dc3 (5'-nucleotidase domain-containing protein 3 [Source:NCBI gene;Acc:103044642])

HSP 1 Score: 137.117 bits (344), Expect = 6.357e-46
Identity = 100/337 (29.67%), Postives = 162/337 (48.07%), Query Frame = 2 
Query:  476 LFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFIN--GSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKE---LAGQLEAAYGT-LGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHES 1468
            +FSL EM LL+ V  +F  H             N+      + +DV++A   +H +G + R     +E Y+   ++   +L +L   G K FL+TNS   +  + M YI+         ++W   FD ++V A KP FF +      R  DK    +++   I  L+ GQIY  G+   F +L G RG  VLY GDHI+ D+       GWRT  +IPE+  E+ +   E+  Y   M    +L ++   + +  D      +++  I+E   +  Q +  + +  GSLFR     T+FS ++ R+AD+Y AS   +++Y L + F  +   L HES
Sbjct:  236 IFSLPEMTLLSCVNDYFMKH-------------NIDYEPVHLYKDVKEAIGDVHVKGIMYRAVEADIEKYICYGEQTHAVLRKLSENGKKMFLITNSPLDFVDRGMNYIVG--------KDWRDLFDVVIVQADKPSFFNDRRKPFRRVTDK---GVLLWDRIHHLEKGQIYKQGNLYEFLRLTGWRGSKVLYFGDHIYSDLADLTLKHGWRTGAIIPELRKEIKIMNTEE--YVHTMTWLQSLTSLLEHMQVHRDPASQA-VIQQWIREREDMRSQAKDIFNSQFGSLFRTYHNPTYFSRRLSRFADIYMASISCLLNYDLKHTFYPRRTPLQHES 545

HSP 2 Score: 67.781 bits (164), Expect = 6.357e-46
Identity = 39/103 (37.86%), Postives = 58/103 (56.31%), Query Frame = 3 
Query:  120 IFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILI-EYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALI--GFYFLTTKLFI 419
            IF N  + ++ I  YGFD DYTLA Y S     L F++ RDILI E+ YP+  + ++Y   F +RGL +D +  + MK D +  I L  +  G + ++ K  I
Sbjct:  103 IFANNEMSLQDIEIYGFDYDYTLAFYSS-NLHTLIFNIARDILIQEHRYPEGLKEYEYIPDFVVRGLHYDVQKALLMKIDAFHYIQLGTVYRGLHPVSDKEVI 204
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004734.1 (pep scaffold:Pmarinus_7.0:GL478541:2134:43818:1 gene:ENSPMAG00000004284.1 transcript:ENSPMAT00000004734.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 371.318 bits (952), Expect = 9.598e-131
Identity = 180/360 (50.00%), Postives = 254/360 (70.56%), Query Frame = 2 
Query:  407 EIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP--DQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            EI   Y NKF++ D++ R YV+NTLF+L E YL A ++ +F N   YI  + GV++ ++FMS++S+ QDVRDA D++H  G LK KTLE +E YV    KLP +L R++   AK FL TNS+Y+YT KIM Y+  +P   Q G   R W +YFD I+VDA+KP FF  G++LR VD ETG+L +G   GPL+ G +YSGGS ++   L+G  GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL +LD  L ++Y  L+ +S+++PD+  ++  IK++   ++  YG +GSLFR+GSR T F+SQ+ RYAD+Y+ASC+N++HYP  Y+FR+  +L+PHES +
Sbjct:  132 EIREQYSNKFIQRDDTERFYVLNTLFNLPETYLYACLVDFFINCTRYISCDTGVKEGDLFMSFKSMFQDVRDAVDWVHIMGTLKEKTLENVEKYVAKEPKLPLLLSRMQEV-AKVFLATNSDYNYTDKIMTYLFDLPHGPQPGRQHRPWQSYFDLIIVDARKPLFFGEGTVLRQVDTETGQLKIGTFTGPLQHGIVYSGGSSDLVCDLLGVNGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELALELHVWTDKSALFEELQRLDIYLADLYKNLDSSSNERPDISSLQKCIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADIYAASCINMLHYPFSYLFRAPHVLMPHESTV 490

HSP 2 Score: 114.39 bits (285), Expect = 9.598e-131
Identity = 52/104 (50.00%), Postives = 73/104 (70.19%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLTTKL 413
            R   HRIFVNRSL +E+IR +G +MDYTLA Y+SP++E L FDLT + L+  GYP E  +F+Y+  F +RG+ FD  YG  +K D YGN+L+ + GF F+  ++
Sbjct:   30 REACHRIFVNRSLAVERIRCFGLEMDYTLAVYRSPEYEALEFDLTIERLVSIGYPQELHNFEYDPEFPIRGMVFDTLYGNLLKVDAYGNLLVCVHGFKFMKPEI 133
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009631.1 (pep scaffold:Pmarinus_7.0:GL476859:39696:56521:1 gene:ENSPMAG00000008682.1 transcript:ENSPMAT00000009631.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 369.007 bits (946), Expect = 1.314e-121
Identity = 186/411 (45.26%), Postives = 275/411 (66.91%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP---DQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAML-THDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSED 1588
            + GFN+  +   EI   YPNKF++ D++ R Y++NTLF+L E YL A ++ +FTN   Y   E GV+  +++MS++S+ QDVR+A D++H +G+LK KTLE LE YV    +LP +L R++  G K FL TNS+Y+YT KIM+Y+   P       + R W +YFD  VVDA+KP FF  G++LR VD +TG L +G + GPL+ G +YSGGS ++   L+G++GKD+LY+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW ++   + EL  LD  L  +Y  L+ +S+++PD+  ++ +IK++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY++S +N+++YP  Y+FR+  +L+PHES +   H+ + E E P           +   ++ LMQ  S D
Sbjct:   91 VHGFNF--MKGPEIREQYPNKFIQRDDTERFYILNTLFNLPETYLYACLVDFFTNCARYKRCETGVKDGDLYMSFKSMFQDVREAMDWVHMQGSLKSKTLENLEKYVVKEPRLPLLLSRMKEVG-KVFLATNSDYNYTNKIMKYLFDYPHGPKPGTSHRPWHSYFDLTVVDARKPLFFAEGTVLRQVDLKTGHLKIGTYTGPLQHGIVYSGGSSDMICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTEKSALFEELQGLDIFLAELYKHLDSSSNERPDISSLQKRIKKVTHDMDMCYGMMGSLFRSGSRPTLFASQVMRYADLYASSFINLLYYPFSYLFRAPHILMPHESTVEHVHNGLGETESP-----------MATRNRLLMQDESTD 487

HSP 2 Score: 114.39 bits (285), Expect = 4.265e-27
Identity = 54/95 (56.84%), Postives = 69/95 (72.63%), Query Frame = 3 
Query:  117 RIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R+FVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF+Y+  F  RGL FD  +G  +K D YGNIL+ + GF F+
Sbjct:    3 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYEALGFELTVERLVNIGYPQELLSFEYDPSFPTRGLVFDAMFGNLLKVDAYGNILVCVHGFNFM 97
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Sea Lamprey
Match: NT5DC2 (5'-nucleotidase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25717])

HSP 1 Score: 93.2041 bits (230), Expect = 6.332e-22
Identity = 65/218 (29.82%), Postives = 111/218 (50.92%), Query Frame = 2 
Query:  614 RDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGS-ILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVK 1264
            +DA   +H +G + +   E LE Y+   ++   +L +L S G K FL+TNS +S+  K M +++          +W   FD I+V A+KP FF + +   R +D ++G L     I  L+ G+IY  G+   F +L G  G  VLY GDHI+ D+       GWRT  ++PE+  E+ +   ++  Y + +    +L  +  ++ +   Q+  V + K
Sbjct:    1 QDAVRDVHVKGLMYKIIEENLEEYIQSGEETYAVLQKLVSYGKKLFLITNSPFSFVNKGMLHMVG--------EDWRELFDVIIVQAEKPAFFTDSAKPFRRLD-DSGCL-QWDKIDKLEKGEIYQQGNLYEFLRLTGWVGTSVLYFGDHIYSDLADLTLRHGWRTGAIVPELEGEIHIINGQE--YTDALTWLQSLTGLLERMQMYRGQEAQVILTK 206
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Nematostella
Match: EDO47687 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLG5])

HSP 1 Score: 396.356 bits (1017), Expect = 1.882e-131
Identity = 194/371 (52.29%), Postives = 263/371 (70.89%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQK-DNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQN-GNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKP-DVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            + GF++  L + E+  +YPNKF++ D SR +++NTLF+L E YLLA ++ +F NH D+   + GV+  D+V ++YQSI QDVR A D++H +G LK+ T+E L+ YV     LP +LDRLR    KTF+LTNS YSYT KIM Y+L   D++  + R WT+YFD++VVDA+KP FF  G+ LR VD ETG L +G++ G LK G IYSGGS E+F KLIG++GKDVLYVGDHIFGDILKSKK  GWRTYLVIPE+  EL VW+  Q  + +L  LD  L   Y  L+ A+ ++P ++  +K  IK+    +E  YG +GSL R+GSR TFF+SQ  RYADLY++SC+N++HYP  Y+FR+   L+PHES +
Sbjct:  114 VHGFDF--LPNLEVRELYPNKFVQADSSRYFILNTLFNLPETYLLACLVDFFDNHPDFKRIKGGVESNDHVMLTYQSIHQDVRAAVDWVHIKGTLKQHTVENLDTYVIKDPHLPKLLDRLRQCRRKTFILTNSGYSYTAKIMSYLLDSADRDEAHKRSWTSYFDFVVVDARKPLFFGEGTALRQVDVETGTLSIGRYAGALKKGHIYSGGSSEVFCKLIGAQGKDVLYVGDHIFGDILKSKKQQGWRTYLVIPELTQELEVWQSRQDVFSKLRDLDIALAETYKNLDSATTEQPNNISQIKRSIKDATQTMEICYGQMGSLLRSGSRQTFFASQAMRYADLYASSCVNLLHYPFGYLFRAPAQLMPHESTV 482

HSP 2 Score: 113.235 bits (282), Expect = 1.770e-26
Identity = 55/95 (57.89%), Postives = 68/95 (71.58%), Query Frame = 3 
Query:  117 RIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            RIFVNRSL M+KI+F+GFDMDYTLA YKSP +E   FDL  + L+  GYP +  SF+Y+  F +RGL+FD E G  +K D YGNIL  + GF FL
Sbjct:   26 RIFVNRSLSMDKIKFFGFDMDYTLAVYKSPVYEAKAFDLVCERLVSIGYPKDLLSFQYDPIFPVRGLFFDCELGNLLKVDTYGNILSCVHGFDFL 120
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Nematostella
Match: EDO41423 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4J0])

HSP 1 Score: 137.117 bits (344), Expect = 3.829e-45
Identity = 97/363 (26.72%), Postives = 172/363 (47.38%), Query Frame = 2 
Query:  398 SHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFING-SILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQK--PDVKIVKMKIKELAGQLEAAYGT-LGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            +H  I  + P  F+      I+ M  +FS  EM LL+ V  +F         + G+  D      +++ +D   A + +H  G +    +  +  Y+  S  L  ML+++R+ G KTFL+TNS Y++  + M +++           W   FD I+  A+KP F+       R VD    +    Q +   + GQIY  G+ + F K  G  G  VLY GDH++ D+       GWRT  VIPE+  E  +       Y + +    TL+++ +++ +   ++    ++  K + ++L  +L+  +    GS+FR     T+F+ ++ R+AD+Y +S  N + Y   ++F  +   LPHE  +
Sbjct:  108 THLAITKLDPYTFLSGPRRTIHHMIDMFSYPEMCLLSNVTEYFN--------QNGIPYDP-----ENLFRDCVQAVESVHASGQMYEAVMNNIGMYLEKSPLLREMLNKIRASGRKTFLITNSPYTFVDRGMDHMVG--------GGWQELFDVIITKARKPTFYNQVYRPFRCVDASEAR-PTWQKVSKFEKGQIYIEGNVDQFIKFTGWYGPSVLYFGDHVYSDLKDPVLHHGWRTGAVIPELERE--IQTTNLPEYQKSVVWLFTLQDLIAEIQVHDREEILSTLEEWKAERRQLRTKLKTLFNPHFGSVFRTYHNPTYFTRRLVRFADIYMSSVENFLQYRDNHVFYPRRAALPHEPVL 446

HSP 2 Score: 63.5438 bits (153), Expect = 3.829e-45
Identity = 35/90 (38.89%), Postives = 50/90 (55.56%), Query Frame = 3 
Query:  120 IFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEY-GYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALI 386
            +F N  L ++ I  YGFD DYTLA+Y S     L +DL    L+ + GYP      KY   F++RGL +D   G+ +K D + NI+L  +
Sbjct:    1 MFANNELSLKYINVYGFDYDYTLASY-SNTLHFLIYDLAVGNLLSFFGYPKGVEGMKYNPEFAVRGLHYDTVNGLLLKIDAFHNIMLGSV 89
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Nematostella
Match: EDO26381 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TC58])

HSP 1 Score: 50.0618 bits (118), Expect = 4.300e-7
Identity = 32/90 (35.56%), Postives = 44/90 (48.89%), Query Frame = 3 
Query:  120 IFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIE-YGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALI 386
            IF N  L ++ I  YGFD DYTLA+Y S     L +DL    L+  +GYP      KY   F+          G+ +K D + NI+L  +
Sbjct:   12 IFANNELSLKYINVYGFDYDYTLASY-SNTLHFLIYDLAVGNLLSFFGYPKGVEGMKYNPEFA----------GLLLKIDAFHNIMLGSV 90
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Match: nt5c2l1 (cytosolic purine 5'-nucleotidase [Source:NCBI gene;Acc:101174682])

HSP 1 Score: 350.903 bits (899), Expect = 2.823e-121
Identity = 172/367 (46.87%), Postives = 251/367 (68.39%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIR-EWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESA 1471
            GF +  L   +I + YPNKF++ D++ R Y++NTLF+LSE Y+ A ++ +FT    Y    KG Q  ++FMSY+S+ QDVRDA D+IH  G LK +T++ LE YV     +P +L R++   AK FL TNS+Y+YT  IM+Y+     + G  +  W +YFD +VVD +KP FF +G++LR VD  TGKL +G + G L+ G +YSGGS +    L+G +GKD+LYVGDHIFGDILKSKK  GW+T+LV+PE+  EL VW ++Q  + EL +LD  L  +Y  L+  S   PD+  ++ +++ L  +++ +YG +GSL R+GS  T F+SQ+ RYADLYS+SC+N++HYP  Y+F + P+L+PHE+A
Sbjct:  111 GFRF--LRGEDIQNYYPNKFIQRDDTERFYILNTLFNLSETYIYACLVDFFTRCNRYTNLLKGFQHGDLFMSYRSMFQDVRDAMDFIHDSGILKEQTIKNLEKYVVRDPNIPVLLTRMKEV-AKVFLATNSDYNYTEAIMRYLFESNVKPGTPKLPWRSYFDLVVVDTRKPLFFGDGTVLRQVDTNTGKLRIGTYTGDLQHGTVYSGGSSDTVCDLLGVKGKDILYVGDHIFGDILKSKKRQGWKTFLVVPELTKELQVWEEKQNLFEELKRLDVFLAELYKHLDSGSRGCPDIGAIQTRMRVLTHRMDMSYGQMGSLLRSGSTQTLFASQLIRYADLYSSSCINLLHYPFNYLFMAPPVLMPHEAA 474

HSP 2 Score: 105.531 bits (262), Expect = 2.823e-121
Identity = 52/116 (44.83%), Postives = 72/116 (62.07%), Query Frame = 3 
Query:   54 VDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            +D   + S+ E   ++  +K +IFVNRSL +E I+ YGFDMDYTL  YKSP +E + F+L RD ++  GYP E   + Y+  F  RGL  D +YG  +K D  GN+L+   GF FL
Sbjct:    1 MDNLASSSSCEPALKTGFEK-KIFVNRSLTLENIKCYGFDMDYTLVAYKSPDYESMGFELLRDRMVSVGYPHEILRYTYDPSFPTRGLVVDTKYGNLLKVDSNGNVLVCTHGFRFL 115
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Match: nt5c2b (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:101161612])

HSP 1 Score: 368.237 bits (944), Expect = 4.252e-120
Identity = 179/372 (48.12%), Postives = 256/372 (68.82%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP--DQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAM 1474
            + GFN+  L   +I   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G LK KT+E +E YV    KL  +L R+    AK FL TNS+Y YT KIM Y+   P   Q G   R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +    L+G++GKD++Y+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW  +   + EL  LD  L  +Y  L+ +S+++PD+  ++ ++K++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+PHES +
Sbjct:  122 VHGFNF--LRGPDIRERYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCDSYTSCETGFKNGDLFMSYKSMFQDVRDAVDWVHFKGTLKEKTVENMEKYVVKDAKLSLLLSRMNEV-AKVFLATNSDYKYTDKIMTYLFDFPHGPQPGTPHRPWQSYFDLILVDARKPMFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDTVCDLLGAKGKDIVYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQGLDVFLAELYKHLDSSSNERPDISTIQRRVKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV 490

HSP 2 Score: 120.168 bits (300), Expect = 3.136e-28
Identity = 59/100 (59.00%), Postives = 70/100 (70.00%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            R   HRIFVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+  F  RGL FD  YG  +K D YGNIL+ + GF FL
Sbjct:   29 REPYHRIFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFVYDPSFPTRGLVFDNMYGNLLKVDAYGNILVCVHGFNFL 128
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Match: nt5c2l1 (cytosolic purine 5'-nucleotidase [Source:NCBI gene;Acc:101174682])

HSP 1 Score: 337.806 bits (865), Expect = 1.244e-109
Identity = 169/378 (44.71%), Postives = 249/378 (65.87%), Query Frame = 2 
Query:  377 GFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEY 1507
            GF +  L   +I + YPNKF++ D++ R Y++NTLF+LSE Y+ A ++ +FT    Y    KG Q  ++FMSY+S+ QDVRDA D+IH  G LK +T++ LE YV     +P +L R++   AK FL TNS+Y+YT  IM+Y+         +  W +YFD +VVD +KP FF +G++LR VD  TGKL +G + G L+ G +YSGGS +    L+G +GKD+LYVGDHIFGDILKSKK  GW+T+LV+PE+  EL VW ++Q  + EL +LD  L  +Y  L+  S   PD+  ++ +++ L  +++ +YG +GSL R+GS  T F+SQ+ RYADLYS+SC+N++HYP  Y+F + P+L+       T  +++   Y
Sbjct:   72 GFRF--LRGEDIQNYYPNKFIQRDDTERFYILNTLFNLSETYIYACLVDFFTRCNRYTNLLKGFQHGDLFMSYRSMFQDVRDAMDFIHDSGILKEQTIKNLEKYVVRDPNIPVLLTRMKEV-AKVFLATNSDYNYTEAIMRYLFESNPGTPKL-PWRSYFDLVVVDTRKPLFFGDGTVLRQVDTNTGKLRIGTYTGDLQHGTVYSGGSSDTVCDLLGVKGKDILYVGDHIFGDILKSKKRQGWKTFLVVPELTKELQVWEEKQNLFEELKRLDVFLAELYKHLDSGSRGCPDIGAIQTRMRVLTHRMDMSYGQMGSLLRSGSTQTLFASQLIRYADLYSSSCINLLHYPFNYLFMAPPVLVSQLLHSCTRKTLLNPIY 445

HSP 2 Score: 76.2554 bits (186), Expect = 2.724e-14
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 3 
Query:  174 MDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            MDYTL  YKSP +E + F+L RD ++  GYP E   + Y+  F  RGL  D +YG  +K D  GN+L+   GF FL
Sbjct:    1 MDYTLVAYKSPDYESMGFELLRDRMVSVGYPHEILRYTYDPSFPTRGLVVDTKYGNLLKVDSNGNVLVCTHGFRFL 76
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Match: nt5c2b (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:101161612])

HSP 1 Score: 285.034 bits (728), Expect = 2.089e-89
Identity = 157/373 (42.09%), Postives = 232/373 (62.20%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDES-RIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVD---KETGKLIVGQHIGPLKLGQIYSGG---SCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNI----ASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLL 1456
            + GFN+  L   +I   YPNKF++ D++ R Y++NTLF+L E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G LK KT+E +E YV    KL  +L R+    AK FL TNS+Y YT KIM Y+   P          +YFD I+VDA+KP FF  G++LR VD   ++T K  +      L L   ++     S +    L+  R  D++Y+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW             D + E+ +S  ++    +S+++PD+  ++ ++K++   ++  YG +GSLFR+GSR T F+SQ+ RYADLY+AS +N+++YP  Y+FR+  +L+
Sbjct:   69 VHGFNF--LRGPDIRERYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCDSYTSCETGFKNGDLFMSYKSMFQDVRDAVDWVHFKGTLKEKTVENMEKYVVKDAKLSLLLSRMNE-VAKVFLATNSDYKYTDKIMTYLFDFPHGP------QSYFDLILVDARKPMFFGEGTVLRQVDTVSRQTEKTFLFSETESLCLIDFFTVSDHRSSDWMLSLL--RLLDIVYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVW------------TDKSCEHTHSHTDVHLDSSSNERPDISTIQRRVKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLV 418

HSP 2 Score: 75.485 bits (184), Expect = 4.634e-14
Identity = 38/76 (50.00%), Postives = 50/76 (65.79%), Query Frame = 3 
Query:  174 MDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFL 401
            +D+T+  YKSP++E L F+LT + L+  GYP E  SF Y+  F  RGL FD  YG  +K D YGNIL+ + GF FL
Sbjct:    1 VDFTVI-YKSPEYESLGFELTVERLVSIGYPQELLSFVYDPSFPTRGLVFDNMYGNLLKVDAYGNILVCVHGFNFL 75
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Match: nt5c2b (5'-nucleotidase, cytosolic II [Source:NCBI gene;Acc:101161612])

HSP 1 Score: 236.498 bits (602), Expect = 2.509e-89
Identity = 120/251 (47.81%), Postives = 163/251 (64.94%), Query Frame = 2 
Query:  491 EMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVP--DQNGNI-REWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIAS 1234
            E YLLA ++ +FTN   Y   E G +  ++FMSY+S+ QDVRDA D++H +G LK KT+E +E YV    KL  +L R+    AK FL TNS+Y YT KIM Y+   P   Q G   R W +YFD I+VDA+KP FF  G++LR VD  TG+L +G + GPL+ G +YSGGS +    L+G++GKD++Y+GDHIFGDILKSKK  GWRT+LVIPE+  EL VW             D + E+ +S  ++++
Sbjct:  142 ETYLLACLVDFFTNCDSYTSCETGFKNGDLFMSYKSMFQDVRDAVDWVHFKGTLKEKTVENMEKYVVKDAKLSLLLSRMNEV-AKVFLATNSDYKYTDKIMTYLFDFPHGPQPGTPHRPWQSYFDLILVDARKPMFFGEGTVLRQVDTTTGRLKIGTYTGPLQHGIVYSGGSSDTVCDLLGAKGKDIVYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWT------------DKSCEHTHSHTDVSA 379

HSP 2 Score: 113.62 bits (283), Expect = 2.509e-89
Identity = 58/109 (53.21%), Postives = 69/109 (63.30%), Query Frame = 3 
Query:   51 YVDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILL 377
            Y D   T       +  R   HRIFVNRSL MEKI+ +GFDMDYTLA YKSP++E L F+LT + L+  GYP E  SF Y+  F  RGL FD  YG  +K D YGNIL+
Sbjct:   12 YADLPATMDGLAMKKYRREPYHRIFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFVYDPSFPTRGLVFDNMYGNLLKVDAYGNILV 120
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Match: SMESG000009496.1 (SMESG000009496.1)

HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0
Identity = 409/419 (97.61%), Postives = 412/419 (98.33%), Query Frame = 2 
Query:  371 IAGFNWILLSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNSEKGLKVS 1627
            +A   +  L+  EIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNSEKGLKVS
Sbjct:  113 LALIGFYFLTTQEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDSMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNSEKGLKVS 531

HSP 2 Score: 259.996 bits (663), Expect = 4.554e-79
Identity = 126/132 (95.45%), Postives = 127/132 (96.21%), Query Frame = 3 
Query:   39 MYNKYVDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLTTKLFIFIRIN 434
            MYNKYVDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLTT+  I I  N
Sbjct:    1 MYNKYVDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGFYFLTTQEIIHIYPN 132
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Match: SMESG000021273.1 (SMESG000021273.1)

HSP 1 Score: 379.022 bits (972), Expect = 1.046e-133
Identity = 189/366 (51.64%), Postives = 251/366 (68.58%), Query Frame = 2 
Query:  395 LSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKG-VQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDS 1489
            +S+ +I   YPNKF+KF++ R+ V NTL++L E YL+A  I  F +    +  + G    DNV ++Y SI++D+RDA D++H    +    L+ LE YV  + KL  ML  L+S GAK FLLTNS+Y+YT KI QYILS    N     W +YFDYIV +A KP+FF +G++LR V+KET  L +G H GP+K G IYSGGS  IFSKLIG+ GKDVLY GDHIFGDILKSKK+VGW+T L++PE+ +E+FVW+ +   + EL  L+  L   Y  L + S + PD   ++ KI E+A  +E AYG  GSLFR+GSR TFFS Q+ RYADLY+ SCLN+I+YP+ YMFR+  ML+PHES +   DS
Sbjct:  121 MSNADIFLQYPNKFVKFEQPRVMVTNTLYNLPETYLIAATIDHFESINHCVKDDTGFCLSDNVTITYSSIIEDIRDAMDFVHGGKEMSDTILQNLEKYVEKTPKLAEMLTHLKSNGAKVFLLTNSDYNYTEKICQYILS--SSNDKNVHWDSYFDYIVTNAGKPRFFFDGTLLRVVNKETKNLEIGHHTGPMKSGVIYSGGSVAIFSKLIGASGKDVLYFGDHIFGDILKSKKVVGWKTCLIVPEIENEIFVWKHKGDLFDELQSLNCQLAETYRDLGLESLESPDTNGMRQKIHEMARTMEEAYGKFGSLFRSGSRQTFFSDQVFRYADLYTNSCLNLIYYPISYMFRAPFMLMPHESTVSHVDS 484

HSP 2 Score: 118.242 bits (295), Expect = 1.046e-133
Identity = 52/97 (53.61%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGF 392
            R  KHRIFVN+SLQ+ KI+F+GFDMDYT+A YKSP+ E + F L +  +I  GYP     F+Y+  F +RGLW DK++G  +K D YGNIL+A  G 
Sbjct:   22 RESKHRIFVNKSLQLRKIKFFGFDMDYTIAMYKSPELEHMTFQLLKTRMINIGYPKAIMDFEYDPSFPIRGLWIDKKFGNLLKMDNYGNILMAFHGL 118
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Match: SMESG000021273.1 (SMESG000021273.1)

HSP 1 Score: 379.022 bits (972), Expect = 1.245e-133
Identity = 189/366 (51.64%), Postives = 251/366 (68.58%), Query Frame = 2 
Query:  395 LSHNEIIHIYPNKFMKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKG-VQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHDS 1489
            +S+ +I   YPNKF+KF++ R+ V NTL++L E YL+A  I  F +    +  + G    DNV ++Y SI++D+RDA D++H    +    L+ LE YV  + KL  ML  L+S GAK FLLTNS+Y+YT KI QYILS    N     W +YFDYIV +A KP+FF +G++LR V+KET  L +G H GP+K G IYSGGS  IFSKLIG+ GKDVLY GDHIFGDILKSKK+VGW+T L++PE+ +E+FVW+ +   + EL  L+  L   Y  L + S + PD   ++ KI E+A  +E AYG  GSLFR+GSR TFFS Q+ RYADLY+ SCLN+I+YP+ YMFR+  ML+PHES +   DS
Sbjct:  121 MSNADIFLQYPNKFVKFEQPRVMVTNTLYNLPETYLIAATIDHFESINHCVKDDTGFCLSDNVTITYSSIIEDIRDAMDFVHGGKEMSDTILQNLEKYVEKTPKLAEMLTHLKSNGAKVFLLTNSDYNYTEKICQYILS--SSNDKNVHWDSYFDYIVTNAGKPRFFFDGTLLRVVNKETKNLEIGHHTGPMKSGVIYSGGSVAIFSKLIGASGKDVLYFGDHIFGDILKSKKVVGWKTCLIVPEIENEIFVWKHKGDLFDELQSLNCQLAETYRDLGLESLESPDTNGMRQKIHEMARTMEEAYGKFGSLFRSGSRQTFFSDQVFRYADLYTNSCLNLIYYPISYMFRAPFMLMPHESTVSHVDS 484

HSP 2 Score: 118.242 bits (295), Expect = 1.245e-133
Identity = 52/97 (53.61%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  102 RSDKHRIFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILLALIGF 392
            R  KHRIFVN+SLQ+ KI+F+GFDMDYT+A YKSP+ E + F L +  +I  GYP     F+Y+  F +RGLW DK++G  +K D YGNIL+A  G 
Sbjct:   22 RESKHRIFVNKSLQLRKIKFFGFDMDYTIAMYKSPELEHMTFQLLKTRMINIGYPKAIMDFEYDPSFPIRGLWIDKKFGNLLKMDNYGNILMAFHGL 118
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Match: SMESG000037078.1 (SMESG000037078.1)

HSP 1 Score: 118.242 bits (295), Expect = 2.855e-40
Identity = 94/349 (26.93%), Postives = 159/349 (45.56%), Query Frame = 2 
Query:  446 DESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFMSYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQH----IGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLN--IASDQKPDVKI---VKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHE 1465
            D  ++  +  LF + EM LL  +I  F ++        G++ D  ++      QDV  A   IH  G +  +    LE Y+  + +L  +L  L+    K FL+TNS++ +    M ++            WT +FD ++  AKKP FF+      +  K    +I GQ     +    + Q Y  G+   F K  G   K++ Y GDHI+ D+       GW T  +I E++ E+ V       Y + +K    +E +  Q     ASD +   +    ++ ++++   +    Y   GSLFR     ++F  +I  YAD+Y++S  N++ YPL + F  +  +LPHE
Sbjct:  187 DADKLSQLLDLFVVPEMNLLGNIIQLFVDN--------GIEFDPAYL-----YQDVSRAVRSIHKSGRMHFEVSNNLEQYIQFNPQLNLLLTLLKDNNKKLFLITNSDFHFVNLGMIHLTQTS-------RWTDFFDVVITSAKKPDFFL------STKKPFRCIINGQSTWSSVRNFSVSQTYKEGNLSDFMKYTGWNAKEIFYFGDHIYADLADVATTQGWTTGAIIREISEEIAVLNT--VGYQQSLKALLLVEKLIYQFQHVKASDARARYEYWSELRCRLRQRTKKQFNPY--FGSLFRTYHNPSYFCQRISVYADVYTSSVNNLMKYPLNHHFYPKRQILPHE 505

HSP 2 Score: 67.3958 bits (163), Expect = 2.855e-40
Identity = 36/87 (41.38%), Postives = 49/87 (56.32%), Query Frame = 3 
Query:  120 IFVNRSLQMEKIRFYGFDMDYTLANYKSPQFEELCFDLTRDILIEY-GYPDEFRSFKYESFFSLRGLWFDKEYGIFMKTDQYGNILL 377
            IF N  + +  I  YGFD DYTLA Y + Q E+  +D  R +L+    YP+   S +    F++RGL +D   G+FMK D Y NI L
Sbjct:   67 IFCNNEVDLSNINCYGFDYDYTLAQY-TKQLEKYIYDEARKLLVATQKYPERILSLEMNPNFAIRGLHYDVRNGLFMKVDAYNNIQL 152
[Close]
BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Match: SMESG000037078.1 (SMESG000037078.1)

HSP 1 Score: 105.531 bits (262), Expect = 8.808e-25
Identity = 80/291 (27.49%), Postives = 134/291 (46.05%), Query Frame = 2 
Query:  620 ATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSGGAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPKFFINGSILRAVDKETGKLIVGQH----IGPLKLGQIYSGGSCEIFSKLIGSRGKDVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMKLDNTLENIYSQLN--IASDQKPDVKI---VKMKIKELAGQLEAAYGTLGSLFRNGSRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHE 1465
            A   IH  G +  +    LE Y+  + +L  +L  L+    K FL+TNS++ +    M ++            WT +FD ++  AKKP FF+      +  K    +I GQ     +    + Q Y  G+   F K  G   K++ Y GDHI+ D+       GW T  +I E++ E+ V       Y + +K    +E +  Q     ASD +   +    ++ ++++   +    Y   GSLFR     ++F  +I  YAD+Y++S  N++ YPL + F  +  +LPHE
Sbjct:   25 AVRSIHKSGRMHFEVSNNLEQYIQFNPQLNLLLTLLKDNNKKLFLITNSDFHFVNLGMIHLTQTS-------RWTDFFDVVITSAKKPDFFL------STKKPFRCIINGQSTWSSVRNFSVSQTYKEGNLSDFMKYTGWNAKEIFYFGDHIYADLADVATTQGWTTGAIIREISEEIAV--LNTVGYQQSLKALLLVEKLIYQFQHVKASDARARYEYWSELRCRLRQRTKKQFNPY--FGSLFRTYHNPSYFCQRISVYADVYTSSVNNLMKYPLNHHFYPKRQILPHE 298
The following BLAST results are available for this feature:
BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NT5C22.118e-13248.835'-nucleotidase, cytosolic II [Source:HGNC Symbol;... [more]
NT5C22.118e-13248.835'-nucleotidase, cytosolic II [Source:HGNC Symbol;... [more]
NT5DC45.698e-8143.895'-nucleotidase domain containing 4 [Source:HGNC S... [more]
NT5C21.850e-5747.895'-nucleotidase, cytosolic II [Source:HGNC Symbol;... [more]
NT5C22.704e-3850.815'-nucleotidase, cytosolic II [Source:HGNC Symbol;... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Y71H10B.19.090e-12543.48pep chromosome:WBcel235:X:2834272:2840292:-1 gene:... [more]
Y71H10B.19.090e-12543.48pep chromosome:WBcel235:X:2834224:2840292:-1 gene:... [more]
Y71H10B.12.486e-12443.48pep chromosome:WBcel235:X:2834272:2840292:-1 gene:... [more]
Y71H10B.12.486e-12443.48pep chromosome:WBcel235:X:2834222:2840292:-1 gene:... [more]
Y71H10B.12.486e-12443.48pep chromosome:WBcel235:X:2834224:2838653:-1 gene:... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG325498.788e-14051.20gene:FBgn0052549 transcript:FBtr0074570[more]
CG325491.414e-13951.20gene:FBgn0052549 transcript:FBtr0074575[more]
CG325491.414e-13951.20gene:FBgn0052549 transcript:FBtr0074574[more]
CG325493.120e-13951.20gene:FBgn0052549 transcript:FBtr0074571[more]
CG325492.113e-13851.20gene:FBgn0052549 transcript:FBtr0340422[more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nt5c2a3.199e-13148.045'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZD... [more]
nt5c2a3.199e-13148.045'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZD... [more]
nt5c2a2.322e-12847.785'-nucleotidase, cytosolic IIa [Source:ZFIN;Acc:ZD... [more]
nt5c2l11.885e-12142.895'-nucleotidase, cytosolic II, like 1 [Source:ZFIN... [more]
nt5c2b1.376e-12048.125'-nucleotidase, cytosolic IIb [Source:ZFIN;Acc:ZD... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nt5c21.340e-13248.835'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c21.157e-13046.985'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
tti12.291e-12243.93TELO2 interacting protein 1 [Source:Xenbase;Acc:XB... [more]
nt5c22.510e-12246.215'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c22.727e-12246.215'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nt5c24.523e-13248.565'-nucleotidase, cytosolic II [Source:MGI Symbol;A... [more]
Nt5c24.957e-13248.565'-nucleotidase, cytosolic II [Source:MGI Symbol;A... [more]
Nt5c24.957e-13248.565'-nucleotidase, cytosolic II [Source:MGI Symbol;A... [more]
Nt5c25.082e-13248.565'-nucleotidase, cytosolic II [Source:MGI Symbol;A... [more]
Nt5c25.962e-13148.565'-nucleotidase, cytosolic II [Source:MGI Symbol;A... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RA22|5NTC_PONAB9.286e-13248.83Cytosolic purine 5'-nucleotidase OS=Pongo abelii O... [more]
sp|P49902|5NTC_HUMAN9.286e-13248.83Cytosolic purine 5'-nucleotidase OS=Homo sapiens O... [more]
sp|Q5EBF1|5NTC_XENTR1.061e-13148.83Cytosolic purine 5'-nucleotidase OS=Xenopus tropic... [more]
sp|O46411|5NTC_BOVIN1.222e-13148.83Cytosolic purine 5'-nucleotidase OS=Bos taurus OX=... [more]
sp|Q3V1L4|5NTC_MOUSE3.000e-13148.56Cytosolic purine 5'-nucleotidase OS=Mus musculus O... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KP959.985e-15554.61Putative cytosolic purine 5-nucleotidase OS=Schist... [more]
A0A4Z2D6P96.319e-15252.91Cytosolic purine 5'-nucleotidase isoform 2 OS=Schi... [more]
A0A1S3I3S51.122e-15150.13cytosolic purine 5'-nucleotidase isoform X3 OS=Lin... [more]
Q5DBL44.924e-15155.50SJCHGC02464 protein OS=Schistosoma japonicum OX=61... [more]
Q2LE751.287e-15055.50Cytosolic 5'-nucleotidase II (Fragment) OS=Schisto... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nt5c2a4.407e-12947.265'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c2a3.216e-12847.265'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c2b4.024e-12047.58cytosolic purine 5'-nucleotidase-like [Source:NCBI... [more]
nt5c2l12.767e-11744.34cytosolic purine 5'-nucleotidase-like [Source:NCBI... [more]
nt5dc36.357e-4629.675'-nucleotidase domain-containing protein 3 [Sourc... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000004734.19.598e-13150.00pep scaffold:Pmarinus_7.0:GL478541:2134:43818:1 ge... [more]
ENSPMAT00000009631.11.314e-12145.26pep scaffold:Pmarinus_7.0:GL476859:39696:56521:1 g... [more]
NT5DC26.332e-2229.825'-nucleotidase domain containing 2 [Source:HGNC S... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO476871.882e-13152.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414233.829e-4526.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO263814.300e-735.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nt5c2l12.823e-12146.87cytosolic purine 5'-nucleotidase [Source:NCBI gene... [more]
nt5c2b4.252e-12048.125'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c2l11.244e-10944.71cytosolic purine 5'-nucleotidase [Source:NCBI gene... [more]
nt5c2b2.089e-8942.095'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
nt5c2b2.509e-8947.815'-nucleotidase, cytosolic II [Source:NCBI gene;Ac... [more]
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BLAST of Cytosolic purine 5' nucleotidase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009496.14.554e-7997.61SMESG000009496.1[more]
SMESG000021273.11.046e-13351.64SMESG000021273.1[more]
SMESG000021273.11.245e-13351.64SMESG000021273.1[more]
SMESG000037078.12.855e-4026.93SMESG000037078.1[more]
SMESG000037078.18.808e-2527.49SMESG000037078.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032987 ID=SMED30032987|Name=Cytosolic purine 5' nucleotidase|organism=Schmidtea mediterranea sexual|type=transcript|length=1821bp
TATATAAATGATAAATTGAAACGGCAAACAACTGAATTATGTATAATAAA
TATGTGGACCCCTTTACAACAAAGTCAAACTCGGAAACTCTACAGCAAAG
CCGGTCAGATAAGCACAGGATTTTTGTTAACAGGAGCTTACAAATGGAAA
AAATCAGATTTTACGGATTCGATATGGATTACACTTTGGCCAATTACAAG
TCTCCACAATTTGAAGAATTGTGCTTTGATCTAACTCGAGATATCCTAAT
AGAATATGGATATCCGGATGAATTTCGGTCATTTAAATACGAATCTTTTT
TTTCTCTCCGGGGCCTCTGGTTTGACAAGGAGTATGGGATTTTCATGAAA
ACTGATCAGTATGGAAATATATTGCTGGCTTTAATTGGATTTTACTTTCT
CACAACGAAATTATTCATATTTATCCGAATAAATTTATGAAATTCGATGA
AAGCAGAATTTACGTTATGAACACCCTTTTTAGTTTGTCTGAAATGTATC
TGTTAGCCAAGGTGATACACTGGTTTACTAATCATAAAGATTATATTGTC
ACTGAAAAAGGCGTTCAGAAAGATAATGTTTTCATGTCTTATCAATCAAT
CATGCAAGACGTAAGAGACGCAACCGACTATATTCATACGGAGGGAAACT
TGAAAAGAAAAACTTTGGAGAAACTAGAATATTACGTACACGTTAGTAAG
AAGTTGCCATTTATGCTGGACAGATTGCGATCTGGAGGAGCAAAGACATT
TTTGCTGACAAACAGTAACTACAGTTATACATTGAAAATTATGCAGTACA
TTCTATCTGTACCGGATCAAAATGGAAACATCAGAGAATGGACAACATAT
TTCGATTACATTGTTGTTGACGCAAAAAAACCAAAGTTTTTCATCAACGG
CTCAATTCTCAGGGCAGTAGACAAAGAAACGGGCAAGCTAATTGTTGGGC
AGCACATTGGTCCATTGAAATTAGGTCAAATCTATTCTGGAGGATCCTGT
GAAATATTTTCCAAACTGATCGGATCTAGAGGAAAGGATGTGCTTTATGT
GGGTGATCATATTTTCGGCGATATTTTGAAATCGAAAAAGATAGTCGGCT
GGCGAACGTATCTTGTCATACCGGAAATGAATCACGAACTGTTTGTCTGG
AGAAAGGAACAGGGCAATTATCACGAATTGATGAAACTGGACAATACTTT
GGAGAACATTTATAGTCAATTAAATATTGCCTCTGATCAGAAACCCGACG
TGAAGATAGTTAAAATGAAAATAAAAGAGTTGGCGGGACAACTAGAAGCG
GCCTATGGCACTCTAGGAAGTTTATTTAGAAATGGATCACGATTGACATT
CTTTTCGTCACAAATTCAAAGATATGCCGATCTTTATTCAGCTTCTTGTT
TGAATGTTATCCATTACCCTTTGTGCTACATGTTTCGGAGTCAACCAATG
CTGTTGCCGCATGAATCCGCCATGCTCACACACGACTCCATGATTGAGGA
CGAATATCCGGATTTGATTTATTTCTCCCGTAGTCCAGAACATTTGAGAC
TCAATGATAAGAATCTTATGCAAGGAAATTCCGAAGACGACGACGATACA
AATTCAGAGAAAGGTTTGAAGGTTTCCTAGACTGTATAAATAATTTTTCA
TTTCCCCTTTATGTTGAATATTCCAATGGAAATAAGCTTTGAGGAATAAT
ATTATATTGCTCCACGTCTTTTTCGATAATATATATTGCACTATGGTCAA
TTATAAATTTTTCTTTGATCATTTCTATTTTTAGGATCATGAAACCGGTA
ATATTGAACCAGATCTCAGTC
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protein sequence of SMED30032987-orf-1

>SMED30032987-orf-1 ID=SMED30032987-orf-1|Name=SMED30032987-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=398bp
MKFDESRIYVMNTLFSLSEMYLLAKVIHWFTNHKDYIVTEKGVQKDNVFM
SYQSIMQDVRDATDYIHTEGNLKRKTLEKLEYYVHVSKKLPFMLDRLRSG
GAKTFLLTNSNYSYTLKIMQYILSVPDQNGNIREWTTYFDYIVVDAKKPK
FFINGSILRAVDKETGKLIVGQHIGPLKLGQIYSGGSCEIFSKLIGSRGK
DVLYVGDHIFGDILKSKKIVGWRTYLVIPEMNHELFVWRKEQGNYHELMK
LDNTLENIYSQLNIASDQKPDVKIVKMKIKELAGQLEAAYGTLGSLFRNG
SRLTFFSSQIQRYADLYSASCLNVIHYPLCYMFRSQPMLLPHESAMLTHD
SMIEDEYPDLIYFSRSPEHLRLNDKNLMQGNSEDDDDTNSEKGLKVS*
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protein sequence of SMED30032987-orf-2

>SMED30032987-orf-2 ID=SMED30032987-orf-2|Name=SMED30032987-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=134bp
MYNKYVDPFTTKSNSETLQQSRSDKHRIFVNRSLQMEKIRFYGFDMDYTL
ANYKSPQFEELCFDLTRDILIEYGYPDEFRSFKYESFFSLRGLWFDKEYG
IFMKTDQYGNILLALIGFYFLTTKLFIFIRINL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002089reproductive organ
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR036412HAD-like_sf
IPR023214HAD_sf
IPR008380HAD-SF_hydro_IG_5-nucl
IPR006928htUSP
Vocabulary: molecular function
TermDefinition
GO:0008233peptidase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008380HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidasePFAMPF057615_nucleotidcoord: 6..344
e-value: 3.3E-111
score: 372.4
IPR008380HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidasePANTHERPTHR12103CYTOSOLIC PURINE 5-NUCLEOTIDASE-RELATEDcoord: 4..363
IPR023214HAD superfamilyGENE3DG3DSA:3.40.50.1000coord: 62..257
e-value: 9.6E-51
score: 173.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 377..397
NoneNo IPR availablePANTHERPTHR12103:SF17CYTOSOLIC PURINE 5'-NUCLEOTIDASEcoord: 4..363
IPR036412HAD-like superfamilySUPERFAMILYSSF56784HAD-likecoord: 2..345