SMED30032966

Overview


Name	SMED30032966
Smed ID	SMED30032966
Length (bp)	584

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30032966 (SMED30032966) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032966 (SMED30032966) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032966 (SMED30032966) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032966 (SMED30032966) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032966

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30032966	SMESG000074194.1	SmedASXL_014179	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
nervous system	SMED30032966	SMESG000074194.1	dd_Smed_v4_12736_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30032966	SMESG000074194.1	dd_Smed_v4_12736_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30032966	SMESG000074194.1	dd_Smed_v4_12736_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30032966	SMESG000077776.1 SMESG000074194.1	OX_Smed_1.0.12944	ox_Smed_v2	PMID:24238224 Kao et al., 2013

Note: Hover over icons to view figure legend

Alignments

SMED30032966 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.003904:27636..28218 -	2879

Homology

[Close]

BLAST of SMED30032966 vs. Ensembl Human
Match: UBE2J2 (ubiquitin conjugating enzyme E2 J2 [Source:HGNC Symbol;Acc:HGNC:19268])

HSP 1 Score: 112.464 bits (280), Expect = 8.861e-31
Identity = 56/163 (34.36%), Postives = 97/163 (59.51%), Query Frame = -1

Query:   54 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 536
            + +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    6 SKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Human
Match: UBE2J2 (ubiquitin conjugating enzyme E2 J2 [Source:HGNC Symbol;Acc:HGNC:19268])

HSP 1 Score: 112.464 bits (280), Expect = 4.365e-30
Identity = 56/163 (34.36%), Postives = 97/163 (59.51%), Query Frame = -1

Query:   54 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 536
            + +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    6 SKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Human
Match: UBE2J2 (ubiquitin conjugating enzyme E2 J2 [Source:HGNC Symbol;Acc:HGNC:19268])

HSP 1 Score: 112.464 bits (280), Expect = 4.365e-30
Identity = 56/163 (34.36%), Postives = 97/163 (59.51%), Query Frame = -1

Query:   54 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 536
            + +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    6 SKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Human
Match: UBE2J2 (ubiquitin conjugating enzyme E2 J2 [Source:HGNC Symbol;Acc:HGNC:19268])

HSP 1 Score: 101.679 bits (252), Expect = 6.898e-26
Identity = 56/179 (31.28%), Postives = 97/179 (54.19%), Query Frame = -1

Query:   54 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEW----------------HFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 536
            + +A   +++RL ++   I  + VPYI      +NI EW                H+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    6 SKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWFKRFSWLSLLSSWDYRHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIK 184

[Close]

BLAST of SMED30032966 vs. Ensembl Human
Match: UBE2J1 (ubiquitin conjugating enzyme E2 J1 [Source:HGNC Symbol;Acc:HGNC:17598])

HSP 1 Score: 88.9669 bits (219), Expect = 9.801e-21
Identity = 47/137 (34.31%), Postives = 77/137 (56.20%), Query Frame = -1

Query:  111 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNR---SWSSVYSPLYIMLSIYSFMLEEGSGS-TDLQKTYCERKQLSTESANY 509
            +RLM+EA E+  +   + H     +N++EWHF + G P++ ++ G Y+G ++ P  YP+K P I + T NGRF V   IC L+ S     +W   +S    +L+I  FM  +G G+   L  T  ER+ L+ +S ++
Sbjct:   13 KRLMKEAAEL-KDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKIC-LSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDF 147

[Close]

BLAST of SMED30032966 vs. Ensembl Celegans
Match: ubc-26 (UBiquitin Conjugating enzyme [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATR7])

HSP 1 Score: 103.99 bits (258), Expect = 6.437e-28
Identity = 55/170 (32.35%), Postives = 92/170 (54.12%), Query Frame = -1

Query:   36 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQM 539
            MTS    V+ +RL ++ + +    VP++  +    NI EW +++ GAP T YE G Y G ++FP ++P K P I + TPNGRF  NT +C +++D +  +W+  ++   I+  + SFM +       L  +  ERK L+ +S  +NL+   F   FP    +I+    +M
Sbjct:    1 MTSGVSTVALQRLKKDYQRLLKEPVPFMKAAPLETNILEWRYIIIGAPKTPYEGGIYMGKLLFPKDFPFKPPAILMLTPNGRFQTNTRLCLSISDYHPDTWNPAWTVSTIITGLMSFMNDNQPTLGSLVTSESERKLLAKKSKQWNLKDKSFCALFPEEADQIREEMEKM 170

[Close]

Query:   36 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQM 539
            MTS    V+ +RL ++ + +    VP++  +    NI EW +++ GAP T YE G Y G ++FP ++P K P I + TPNGRF  NT +C +++D +  +W+  ++   I+  + SFM +       L  +  ERK L+ +S  +NL+   F   FP    +I+    +M
Sbjct:    1 MTSGVSTVALQRLKKDYQRLLKEPVPFMKAAPLETNILEWRYIIIGAPKTPYEGGIYMGKLLFPKDFPFKPPAILMLTPNGRFQTNTRLCLSISDYHPDTWNPAWTVSTIITGLMSFMNDNQPTLGSLVTSESERKLLAKKSKQWNLKDKSFCALFPEEADQIREEMEKM 170

[Close]

BLAST of SMED30032966 vs. Ensembl Celegans
Match: ubc-26 (UBiquitin Conjugating enzyme [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATR7])

HSP 1 Score: 104.375 bits (259), Expect = 1.344e-27
Identity = 55/170 (32.35%), Postives = 92/170 (54.12%), Query Frame = -1

Query:   36 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQM 539
            MTS    V+ +RL ++ + +    VP++  +    NI EW +++ GAP T YE G Y G ++FP ++P K P I + TPNGRF  NT +C +++D +  +W+  ++   I+  + SFM +       L  +  ERK L+ +S  +NL+   F   FP    +I+    +M
Sbjct:    1 MTSGVSTVALQRLKKDYQRLLKEPVPFMKAAPLETNILEWRYIIIGAPKTPYEGGIYMGKLLFPKDFPFKPPAILMLTPNGRFQTNTRLCLSISDYHPDTWNPAWTVSTIITGLMSFMNDNQPTLGSLVTSESERKLLAKKSKQWNLKDKSFCALFPEEADQIREEMEKM 170

[Close]

Query:   36 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQM 539
            MTS    V+ +RL ++ + +    VP++  +    NI EW +++ GAP T YE G Y G ++FP ++P K P I + TPNGRF  NT +C +++D +  +W+  ++   I+  + SFM +       L  +  ERK L+ +S  +NL+   F   FP    +I+    +M
Sbjct:    1 MTSGVSTVALQRLKKDYQRLLKEPVPFMKAAPLETNILEWRYIIIGAPKTPYEGGIYMGKLLFPKDFPFKPPAILMLTPNGRFQTNTRLCLSISDYHPDTWNPAWTVSTIITGLMSFMNDNQPTLGSLVTSESERKLLAKKSKQWNLKDKSFCALFPEEADQIREEMEKM 170

[Close]

BLAST of SMED30032966 vs. Ensembl Celegans
Match: ubc-15 (UBiquitin Conjugating enzyme [Source:UniProtKB/TrEMBL;Acc:A0A1T5HUL4])

HSP 1 Score: 98.2117 bits (243), Expect = 2.932e-25
Identity = 49/161 (30.43%), Postives = 89/161 (55.28%), Query Frame = -1

Query:   60 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 536
               A   + RRL ++  ++  + V  I      +NI EWH+ L G+P+T +  G+Y+G +IF  N+P   P I + TPNGRF  NT +C +++D +  SW+  ++   I++ ++SFM E    +  +  T  +++  +  S  +N++  EFR +FP  ++ 
Sbjct:   13 AGTASSSAVRRLQKDYAKLMQDPVDGIKALPNEDNILEWHYCLRGSPDTPFYGGYYWGKVIFKENFPWSPPAITMITPNGRFQTNTRLCLSISDYHPESWNPGWTVSAILIGLHSFMNENSPAAGSIAGTPQDQRMYAAASKEFNVKYREFRRFFPQFVKD 173

[Close]

BLAST of SMED30032966 vs. Ensembl Fly
Match: CG5823 (gene:FBgn0038515 transcript:FBtr0321266)

HSP 1 Score: 94.3597 bits (233), Expect = 2.725e-23
Identity = 47/162 (29.01%), Postives = 91/162 (56.17%), Query Frame = -1

Query:   54 SKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 533
             + +  +  R+ ++   +  + +PYI      NNI EWH+ ++G  ++ Y  G+Y+G ++FP  +P K P I + TPNGRF  NT +C +++D +  +W+  +    I+  + SFMLE       ++ +  +++  + +S  +NL+   F E FP I+++I+
Sbjct:    9 GRKQPTAVSRMKQDYMRLKRDPLPYITAEPLPNNILEWHYCVKGPEDSPYYGGYYHGTLLFPREFPFKPPSIYMLTPNGRFKTNTRLCLSISDFHPDTWNPTWCVGTILTGLLSFMLESTPTLGSIESSNYDKQMFAQKSLAFNLRNTNFCELFPEIVEEIK 170

[Close]

BLAST of SMED30032966 vs. Ensembl Fly
Match: CG5823 (gene:FBgn0038515 transcript:FBtr0083505)

HSP 1 Score: 94.3597 bits (233), Expect = 2.725e-23
Identity = 47/162 (29.01%), Postives = 91/162 (56.17%), Query Frame = -1

Query:   54 SKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 533
             + +  +  R+ ++   +  + +PYI      NNI EWH+ ++G  ++ Y  G+Y+G ++FP  +P K P I + TPNGRF  NT +C +++D +  +W+  +    I+  + SFMLE       ++ +  +++  + +S  +NL+   F E FP I+++I+
Sbjct:    9 GRKQPTAVSRMKQDYMRLKRDPLPYITAEPLPNNILEWHYCVKGPEDSPYYGGYYHGTLLFPREFPFKPPSIYMLTPNGRFKTNTRLCLSISDFHPDTWNPTWCVGTILTGLLSFMLESTPTLGSIESSNYDKQMFAQKSLAFNLRNTNFCELFPEIVEEIK 170

[Close]

BLAST of SMED30032966 vs. Ensembl Fly
Match: Ubc87F (gene:FBgn0267383 transcript:FBtr0082838)

HSP 1 Score: 56.225 bits (134), Expect = 6.275e-10
Identity = 40/148 (27.03%), Postives = 74/148 (50.00%), Query Frame = -1

Query:  144 AEIVSSRRLMREAEEICTNSVPYIHFS---IANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT----PNGRFLVNTNICALND----------SNRSWSSVYSPLYIMLSIYSFML---EEGSGSTDLQKTYCE 527
            +E+ +S  L R+  E+  +  P   FS   +++++I++W  V+ G P+T YE GF+   +IFP  YP++ P+++  T    PN     +  I  L++          +   W  V++   I+LS+ S +    +E + + D  K Y E
Sbjct:    2 SELQASLLLNRQLSELQRH--PVEGFSAGLVSDSDIFKWEVVIIGPPDTLYEGGFFKAHLIFPKEYPLRPPKMKFITEIWHPNIDKAGDVCISILHEPGDDKWGYEKAEERWLPVHTVETILLSVISMLTDPNDESAANVDAAKEYRE 147

[Close]

BLAST of SMED30032966 vs. Ensembl Fly
Match: Ubc2 (gene:FBgn0015320 transcript:FBtr0080115)

HSP 1 Score: 53.9138 bits (128), Expect = 9.969e-9
Identity = 31/117 (26.50%), Postives = 57/117 (48.72%), Query Frame = -1

Query:  186 SKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 533
            S+A   S++R+ +E  EI  +  P        +N+YEW   + G P + YE G ++  + F   YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   81 SRALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 197

[Close]

BLAST of SMED30032966 vs. Ensembl Fly
Match: Ubc2 (gene:FBgn0015320 transcript:FBtr0345437)

HSP 1 Score: 53.9138 bits (128), Expect = 9.969e-9
Identity = 31/117 (26.50%), Postives = 57/117 (48.72%), Query Frame = -1

Query:  186 SKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 533
            S+A   S++R+ +E  EI  +  P        +N+YEW   + G P + YE G ++  + F   YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   81 SRALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 197

[Close]

BLAST of SMED30032966 vs. Ensembl Zebrafish
Match: ube2j2 (ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) [Source:ZFIN;Acc:ZDB-GENE-070410-28])

HSP 1 Score: 112.079 bits (279), Expect = 3.547e-30
Identity = 54/164 (32.93%), Postives = 99/164 (60.37%), Query Frame = -1

Query:   54 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 539
            +  +A   +++RL ++   I  + VPYI      +NI EWH+++ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+++S  +N++   F E FP+++++I+
Sbjct:    5 LNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYLVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLASQSLAFNIKDKVFCELFPDVVEEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Zebrafish
Match: ube2j1 (ubiquitin-conjugating enzyme E2, J1 [Source:ZFIN;Acc:ZDB-GENE-040426-2853])

HSP 1 Score: 92.0485 bits (227), Expect = 3.561e-22
Identity = 48/137 (35.04%), Postives = 78/137 (56.93%), Query Frame = -1

Query:  111 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNR---SWSSVYSPLYIMLSIYSFMLEEGSGS-TDLQKTYCERKQLSTESANY 509
            +RLM+EA E+  +   + H     +N++EWHF + G P++ ++ G Y+G ++ P  YP+K P I + TPNGRF V   IC L+ S     +W   +S    +++I  FM  +G G+   L  T  ERK L+ +S ++
Sbjct:   13 KRLMKEAAEL-RDPTEHYHAQPLEDNLFEWHFSVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTPNGRFEVGKKIC-LSISGHHPETWQPSWSIRTALIAIIGFMPTKGEGAIGSLDYTPEERKALAKKSQDF 147

[Close]

BLAST of SMED30032966 vs. Ensembl Zebrafish
Match: ube2j2 (ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) [Source:ZFIN;Acc:ZDB-GENE-070410-28])

HSP 1 Score: 82.0333 bits (201), Expect = 4.286e-20
Identity = 39/106 (36.79%), Postives = 63/106 (59.43%), Query Frame = -1

Query:  228 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYS 539
            +  +A   +++RL ++   I  + VPYI      +NI EWH+++ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S
Sbjct:    6 LNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYLVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWS 111

[Close]

BLAST of SMED30032966 vs. Ensembl Zebrafish
Match: ube2e1 (ubiquitin-conjugating enzyme E2E 1 [Source:ZFIN;Acc:ZDB-GENE-071004-16])

HSP 1 Score: 58.151 bits (139), Expect = 2.742e-10
Identity = 32/118 (27.12%), Postives = 58/118 (49.15%), Query Frame = -1

Query:  186 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 536
            TSK    S++R+ +E  +I  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   43 TSKLLSTSAKRIQKELADIMLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 160

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BLAST of SMED30032966 vs. Ensembl Zebrafish
Match: ube2e2 (ubiquitin-conjugating enzyme E2E 2 [Source:ZFIN;Acc:ZDB-GENE-040801-237])

HSP 1 Score: 56.9954 bits (136), Expect = 6.372e-10
Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = -1

Query:  186 VSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTN-ICALNDSNRSWSSVYSPLYIMLSIYSFMLE 518
             S++R+ +E  EI  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   55 TSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 166

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BLAST of SMED30032966 vs. Ensembl Xenopus
Match: ube2j1 (ubiquitin conjugating enzyme E2 J1 [Source:NCBI gene;Acc:496585])

HSP 1 Score: 90.5077 bits (223), Expect = 2.273e-21
Identity = 49/137 (35.77%), Postives = 76/137 (55.47%), Query Frame = -1

Query:  111 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNR---SWSSVYSPLYIMLSIYSFMLEEGSGS-TDLQKTYCERKQLSTESANY 509
            +RLM+EA E+  +   + H     +N++EWHF + G P++ ++ G Y+G ++ P  YP+K P I + T NGRF V   IC L+ S     +W   +S    +L+I  FM  +G G+   L  T  ERK L+  S +Y
Sbjct:   35 KRLMKEAAEL-RDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKIC-LSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERKALAKRSQDY 169

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BLAST of SMED30032966 vs. Ensembl Xenopus
Match: ube2j2 (ubiquitin conjugating enzyme E2 J2 [Source:NCBI gene;Acc:734006])

HSP 1 Score: 84.3445 bits (207), Expect = 7.766e-20
Identity = 40/115 (34.78%), Postives = 71/115 (61.74%), Query Frame = -1

Query:   54 TAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 392
            T YE G+Y+G ++FP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    2 TPYEGGYYHGKLVFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAAQSLTFNLKDKVFCELFPEVVEEIK 116

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BLAST of SMED30032966 vs. Ensembl Xenopus
Match: ube2g2 (ubiquitin conjugating enzyme E2 G2 [Source:NCBI gene;Acc:549952])

HSP 1 Score: 59.3066 bits (142), Expect = 7.466e-11
Identity = 42/154 (27.27%), Postives = 70/154 (45.45%), Query Frame = -1

Query:  102 RRLMREAEEICTNSVPYI-HFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT--------PNGRFLVNTNICALND------SNRSWSSVYSPLYIMLSIYSFMLE---EGSGSTDLQKTYCERKQLSTESANYNLQ 509
            +RLM E +++  N    I    I   N +EW  ++ G  +T +E G +  I+ FPL+YP+  P++R           P+GR  ++      +D      S   WS V S   I+LS+ S + E   E   + D  K + E ++   + A   +Q
Sbjct:    7 KRLMAEYKQLTLNPPEGIVAGPINEENFFEWEALIMGPEDTCFECGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWREDREQFNKIARQTVQ 160

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BLAST of SMED30032966 vs. Ensembl Xenopus
Match: nog2 (noggin 2 [Source:Xenbase;Acc:XB-GENE-487602])

HSP 1 Score: 57.3806 bits (137), Expect = 8.662e-10
Identity = 34/124 (27.42%), Postives = 60/124 (48.39%), Query Frame = -1

Query:  186 KLVFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTN-ICALNDSNRSWSSVYSPLYIMLSIYSFMLE 554
            KL    T+K    S++R+ +E  EI  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   50 KLSSKTTAKLS-TSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 172

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BLAST of SMED30032966 vs. Ensembl Xenopus
Match: cox5a (cytochrome c oxidase subunit Va [Source:Xenbase;Acc:XB-GENE-973617])

HSP 1 Score: 56.9954 bits (136), Expect = 9.643e-10
Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = -1

Query:  186 VSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 518
             S++R+ +E  EI  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   55 TSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 166

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BLAST of SMED30032966 vs. Ensembl Mouse
Match: Ube2j2 (ubiquitin-conjugating enzyme E2J 2 [Source:MGI Symbol;Acc:MGI:2153608])

HSP 1 Score: 112.079 bits (279), Expect = 4.123e-30
Identity = 57/161 (35.40%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 168

[Close]

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 168

[Close]

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Mouse
Match: Ube2j2 (ubiquitin-conjugating enzyme E2J 2 [Source:MGI Symbol;Acc:MGI:2153608])

HSP 1 Score: 112.079 bits (279), Expect = 5.114e-30
Identity = 57/161 (35.40%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:   20 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 180

[Close]

BLAST of SMED30032966 vs. Ensembl Mouse
Match: Ube2j2 (ubiquitin-conjugating enzyme E2J 2 [Source:MGI Symbol;Acc:MGI:2153608])

HSP 1 Score: 112.079 bits (279), Expect = 5.114e-30
Identity = 57/161 (35.40%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:   20 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 180

[Close]

BLAST of SMED30032966 vs. UniProt/SwissProt
Match: sp|Q9SHI7|UBC34_ARATH (Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana OX=3702 GN=UBC34 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 4.635e-31
Identity = 56/158 (35.44%), Postives = 91/158 (57.59%), Query Frame = -1

Query:   60 AEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 527
            AE    +RL +E   +C   V ++    + N+I EWH+VLEG+  T +  GFYYG + FP  YP K P I + TPNGRF+    IC +++D +  SW+ ++S   I+  + SFM++    +  +  T  E+++L+  S  +N + P FR+ FP  ++K
Sbjct:    2 AEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVNTTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEK 159

[Close]

BLAST of SMED30032966 vs. UniProt/SwissProt
Match: sp|Q8N2K1|UB2J2_HUMAN (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 112.464 bits (280), Expect = 2.096e-29
Identity = 56/163 (34.36%), Postives = 97/163 (59.51%), Query Frame = -1

Query:   54 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 536
            + +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    6 SKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. UniProt/SwissProt
Match: sp|Q6P073|UB2J2_MOUSE (Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus OX=10090 GN=Ube2j2 PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.884e-29
Identity = 57/161 (35.40%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   +KQL+ +S  +NL+   F E FP ++++I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. UniProt/SwissProt
Match: sp|Q2TA03|UB2J2_BOVIN (Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus OX=9913 GN=UBE2J2 PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 4.856e-29
Identity = 56/161 (34.78%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+ +S  +NL+   F E FP ++++I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAAQSLVFNLKDKVFCELFPEVVEEIK 168

[Close]

BLAST of SMED30032966 vs. UniProt/SwissProt
Match: sp|Q9FK29|UBC33_ARATH (Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana OX=3702 GN=UBC33 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.930e-28
Identity = 54/158 (34.18%), Postives = 90/158 (56.96%), Query Frame = -1

Query:   60 AEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 527
            AE    +RL +E   +C   V ++    + N+I EWH+VLEG+  T +  GFYYG + FP  YP K P I + TPNGRF+    IC +++D +  SW+ ++S   I+  + SFM++    +  +  +  E+++L+  S  +N +   FR+ FP  ++K
Sbjct:    2 AEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEK 159

[Close]

BLAST of SMED30032966 vs. TrEMBL
Match: A0A1Y1HJC5 (Ubiquitin-conjugating enzyme OS=Klebsormidium nitens OX=105231 GN=KFL_000020240 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.962e-31
Identity = 59/152 (38.82%), Postives = 94/152 (61.84%), Query Frame = -1

Query:   60 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 509
            RRL +E +++C    P I    + + I EWH+VLEG+  TA+E G Y+G +IFP +YP K+P IR+ TPNGRF  N  IC ++ D +  +W+ ++S   I+  + SFM ++   S  +  T  E+KQL+  S  +NL+ P F++ FP  +++
Sbjct:    9 RRLQKEFKQLCKEPPPNIKAKPSPDQILEWHYVLEGSAGTAFEGGVYWGKVIFPKDYPFKAPSIRMVTPNGRFAPNAKICMSMTDFHPETWNPMWSVASILTGLLSFMNDDVLTSGSVSSTAAEKKQLAAVSLEWNLKSPIFKKLFPEYVEE 160

[Close]

BLAST of SMED30032966 vs. TrEMBL
Match: A0A0K9S3W6 (UBIQUITIN_CONJUGAT_2 domain-containing protein OS=Spinacia oleracea OX=3562 GN=SOVF_001600 PE=4 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 6.911e-31
Identity = 58/158 (36.71%), Postives = 95/158 (60.13%), Query Frame = -1

Query:   60 AEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 527
            AE    +RL +E   +C   V ++    + NNI EW++VLEG+  T +  G+YYG + FP  YP K P IR+ TPNGRF+ +T IC +++D +  SW+ ++S   I+  + SFM+++   +  +  +  E++ L+  S  YN +IP FR+ FP  ++K
Sbjct:    2 AEKACVKRLQKEYRALCKEPVSHVEARPSANNILEWYYVLEGSEGTPFAGGYYYGRITFPSEYPFKPPSIRMTTPNGRFMTDTKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDKSPTTGSVSTSAEEKQSLAKTSLAYNCKIPTFRKLFPEYVEK 159

[Close]

BLAST of SMED30032966 vs. TrEMBL
Match: A0A0R3TKV8 (UBIQUITIN_CONJUGAT_2 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS7832 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.261e-30
Identity = 55/158 (34.81%), Postives = 101/158 (63.92%), Query Frame = -1

Query:   51 VSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQS 518
            V+ +RL ++   +  + +P+I     N+NI+EWH+++ G P++ Y+ G+Y+G +IFP  YP + P I + TP+GRF+ NT +C +++D +  +W+  +S   I+  + SFMLE+      +  +  E++QL+ ES  YN++ P F E FP+++ ++QS
Sbjct:   11 VAVQRLQQDYVRLARDPIPFIVAHPLNSNIFEWHYLVLGPPDSPYQGGYYHGKLIFPKEYPFRPPSIYMLTPSGRFMCNTRLCLSISDFHPDTWNPAWSVSTILTGLLSFMLEDTPTMGSVSTSLSEKRQLAKESLEYNMKNPIFVELFPDVVTELQS 168

[Close]

BLAST of SMED30032966 vs. TrEMBL
Match: A0A383WB10 (UBIQUITIN_CONJUGAT_2 domain-containing protein OS=Tetradesmus obliquus OX=3088 GN=BQ4739_LOCUS14954 PE=4 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 1.669e-30
Identity = 60/151 (39.74%), Postives = 92/151 (60.93%), Query Frame = -1

Query:   60 RLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQK 506
            RL +E + +    VP+I      +N+ EWHFVLEGAP T +E G Y+G ++FP NYP K P I + TPNGRF VNT +C ++ D +  SW+ ++S   I+  + SFM +    +  +  +  E+++L+ ES  YN +   FR+ FPN L++
Sbjct:   11 RLQKEYKSLLREPVPHIQAHPVPSNLLEWHFVLEGAPATDFEGGVYHGKLVFPPNYPFKPPSISMLTPNGRFAVNTKLCLSITDYHPESWNPMWSVGTILTGLLSFMYDNQPTTGSITSSKAEKQRLAKESLAYNAKNATFRKLFPNWLEE 161

[Close]

BLAST of SMED30032966 vs. TrEMBL
Match: A8JEV0 (Predicted protein (Fragment) OS=Chlamydomonas reinhardtii OX=3055 GN=CHLREDRAFT_132639 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.503e-30
Identity = 55/152 (36.18%), Postives = 94/152 (61.84%), Query Frame = -1

Query:   57 RLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKI 506
            RL +E + +  + VP+I    + +N+ EWHFVLEGA  T Y+ G Y+G ++FP +YP K P I + TPNGRF  NT +C ++ D +  SW+ ++S   I+  + SFM +  + +  +  +  E+++L+ ES  +N + P FR+ FP  ++++
Sbjct:    9 RLQKEYKSLLKDPVPHITAHPSPSNLLEWHFVLEGAKGTEYDGGVYHGKLVFPADYPFKPPSISLFTPNGRFATNTKLCLSMTDYHPESWNPMWSVGTILTGLLSFMYDTQATTGSITTSRAEKERLAAESLAFNAKNPTFRKLFPAWIEEL 160

[Close]

BLAST of SMED30032966 vs. Ensembl Cavefish
Match: ube2j2 (ubiquitin conjugating enzyme E2 J2 [Source:NCBI gene;Acc:103045603])

HSP 1 Score: 111.309 bits (277), Expect = 6.606e-30
Identity = 54/164 (32.93%), Postives = 98/164 (59.76%), Query Frame = -1

Query:   54 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 539
            +  +A   +++RL ++   I  + VPYI      +NI EWH+++ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QL+++S  +N++   F E FP+++ +I+
Sbjct:    5 INKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYLVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLASQSLAFNIKDKVFCELFPDVVDEIK 168

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BLAST of SMED30032966 vs. Ensembl Cavefish
Match: ube2j2 (ubiquitin conjugating enzyme E2 J2 [Source:NCBI gene;Acc:103045603])

HSP 1 Score: 93.2041 bits (230), Expect = 3.810e-23
Identity = 50/150 (33.33%), Postives = 86/150 (57.33%), Query Frame = -1

Query:  120 MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEG--------SGSTDLQKTYCERKQLSTES 539
            +  +A   +++RL ++   I  + VPYI      +NI EWH+++ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G        S  T++++    +++LS+ S
Sbjct:    5 INKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYLVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTEIKQKQRVQEELSSRS 154

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BLAST of SMED30032966 vs. Ensembl Cavefish
Match: ube2j1 (ubiquitin conjugating enzyme E2 J1 [Source:NCBI gene;Acc:103030064])

HSP 1 Score: 89.7373 bits (221), Expect = 3.322e-21
Identity = 45/140 (32.14%), Postives = 78/140 (55.71%), Query Frame = -1

Query:   99 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSN--RSWSSVYSPLYIMLSIYSFMLEEGSGS-TDLQKTYCERKQLSTESANYNLQI 509
            +RLM+EA E+  +   + H     +N++EWHF + G P++ ++ G Y+G ++ P  YP+K P I + TPNGRF V   IC     +   +W   +S    +++I  FM  +G G+   L  T  ER+ L+ +S ++  ++
Sbjct:   13 KRLMKEAAEL-RDPTEHYHAQPLEDNLFEWHFSVRGPPDSDFDGGIYHGRIVLPPEYPMKPPSIILLTPNGRFEVGKKICLSISGHHPETWQPSWSIRTALIAIIGFMPTKGEGAIGSLDYTPEERRALAKKSLDFCCEM 151

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BLAST of SMED30032966 vs. Ensembl Cavefish
Match: ube2e1 (ubiquitin-conjugating enzyme E2E 1 [Source:ZFIN;Acc:ZDB-GENE-071004-16])

HSP 1 Score: 58.9214 bits (141), Expect = 9.479e-11
Identity = 35/133 (26.32%), Postives = 63/133 (47.37%), Query Frame = -1

Query:  141 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCER 536
            TSK    S++R+ +E  +I  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +   G    +K  C +
Sbjct:   44 TSKLLSTSAKRIQKELADIMLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPG----KKLLCAK 172

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BLAST of SMED30032966 vs. Ensembl Cavefish
Match: ube2e3 (ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) [Source:ZFIN;Acc:ZDB-GENE-030131-408])

HSP 1 Score: 57.3806 bits (137), Expect = 3.887e-10
Identity = 30/111 (27.03%), Postives = 55/111 (49.55%), Query Frame = -1

Query:  186 SSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 515
            S++R+ +E  EI  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   47 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 157

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BLAST of SMED30032966 vs. Ensembl Sea Lamprey
Match: ube2g2 (ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) [Source:ZFIN;Acc:ZDB-GENE-050417-288])

HSP 1 Score: 53.5286 bits (127), Expect = 9.071e-10
Identity = 31/121 (25.62%), Postives = 54/121 (44.63%), Query Frame = -1

Query:  135 IANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT--------PNGRFLVNTNICALND------SNRSWSSVYSPLYIMLSIYSFMLE---EGSGSTDLQKTYCERKQ 446
            +   N +EW  ++ G   T +E G +  ++ FP +YP+  P++R           P+GR  ++      +D      S   WS V S   I+LS+ S + E   E   + D  K + E ++
Sbjct:    3 VDEENFFEWEALIMGPEGTCFEGGVFPAVLTFPPDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWREDRE 123

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BLAST of SMED30032966 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008817.1 (pep scaffold:Pmarinus_7.0:GL476329:1748443:1760143:-1 gene:ENSPMAG00000007968.1 transcript:ENSPMAT00000008817.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.0542 bits (105), Expect = 1.096e-6
Identity = 32/112 (28.57%), Postives = 53/112 (47.32%), Query Frame = -1

Query:  186 SSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRI--KTPNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFMLE 515
            + RRLMR+ + +  +    +  + + NNI  W+ V+ G   T +E+G +   + F   YP K P ++   K  +     + +IC     NR WS  Y    I+ SI S + E
Sbjct:    5 ARRRLMRDFKRLQEDPPAGVSGAPSENNIMLWNAVIFGPEGTPFEDGTFKLTLEFTEEYPNKPPTVKFVSKMFHPNVYADGSICLDILQNR-WSPTYDVSSILTSIQSLLDE 115

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BLAST of SMED30032966 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010378.1 (pep scaffold:Pmarinus_7.0:GL477318:26908:31295:-1 gene:ENSPMAG00000009396.1 transcript:ENSPMAT00000010378.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.669 bits (104), Expect = 1.754e-6
Identity = 28/114 (24.56%), Postives = 54/114 (47.37%), Query Frame = -1

Query:  186 SRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEI----RIKTPNGRFLVNTNICALNDSNRS-WSSVYSPLYIMLSIYSFMLE 512
            +R L +E  ++  +           ++++ W   + G  ++ Y+ G ++  + FP +YP K P++    RI  PN    +N+N     D  RS WS   +   ++LSI S + +
Sbjct:    4 TRPLHQELNDLGRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPN----INSNGSICLDILRSQWSPALTISKVLLSICSLLCD 113

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BLAST of SMED30032966 vs. Ensembl Yeast
Match: UBC6 (Ubiquitin-conjugating enzyme involved in ERAD; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; ER-associated protein degradation is also known as ERAD [Source:SGD;Acc:S000000902])

HSP 1 Score: 97.8265 bits (242), Expect = 1.114e-25
Identity = 52/155 (33.55%), Postives = 90/155 (58.06%), Query Frame = -1

Query:   60 SSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYN-LQIPEFREYFPNILQK 515
            + +RL +E + +  N  PYI      +NI EWH+++ G  +T Y+ G Y+G + FP +YP K P IR+ TPNGRF  NT +C +++D +  +W+  +S   I+  + SFM  + + +  +  +  ++K L+  S +YN  Q   F+  FP ++Q+
Sbjct:    6 AHKRLTKEYKLMVENPPPYILARPNEDNILEWHYIITGPADTPYKGGQYHGTLTFPSDYPYKPPAIRMITPNGRFKPNTRLCLSMSDYHPDTWNPGWSVSTILNGLLSFMTSDEATTGSITTSDHQKKTLARNSISYNTFQNVRFKLIFPEVVQE 160

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BLAST of SMED30032966 vs. Ensembl Yeast
Match: UBC7 (Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly [Source:SGD;Acc:S000004624])

HSP 1 Score: 57.3806 bits (137), Expect = 3.345e-11
Identity = 34/126 (26.98%), Postives = 61/126 (48.41%), Query Frame = -1

Query:  186 VSSRRLMREAEEICTNSVPYI-HFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT--------PNGRFLVNTNICALNDSN------RSWSSVYSPLYIMLSIYSFMLE 518
             + +RL++E +++  +S P I     + NNI+ W  +++G P+T Y +G +   + FP +YP+  P++            PNG   ++      +D N        WS V S   I+LS+ S + E
Sbjct:    4 TAQKRLLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSPPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSE 129

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BLAST of SMED30032966 vs. Ensembl Yeast
Match: UBC13 (E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus [Source:SGD;Acc:S000002499])

HSP 1 Score: 50.0618 bits (118), Expect = 1.137e-8
Identity = 29/110 (26.36%), Postives = 58/110 (52.73%), Query Frame = -1

Query:  192 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT----PNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFM 509
            +R+++E E++ ++ VP I     ++N+  +   +EG   + YE+G +   +  P +YP+++P++R  T    PN   L    IC L+    +WS       ++LSI + +
Sbjct:    6 KRIIKETEKLVSDPVPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRL--GRIC-LDVLKTNWSPALQIRTVLLSIQALL 112

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BLAST of SMED30032966 vs. Ensembl Yeast
Match: UBC4 (Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication [Source:SGD;Acc:S000000286])

HSP 1 Score: 48.1358 bits (113), Expect = 5.095e-8
Identity = 30/114 (26.32%), Postives = 53/114 (46.49%), Query Frame = -1

Query:  186 SSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT----PNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFMLE 515
            SS+R+ +E  ++  +           +++Y W   + G  ++ Y  G ++  + FP +YP K P+I   T    PN     N NIC L+     WS   +   ++LSI S + +
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPN--INANGNIC-LDILKDQWSPALTLSKVLLSICSLLTD 113

[Close]

BLAST of SMED30032966 vs. Ensembl Yeast
Match: RAD6 (Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p [Source:SGD;Acc:S000003026])

HSP 1 Score: 48.1358 bits (113), Expect = 9.098e-8
Identity = 37/147 (25.17%), Postives = 66/147 (44.90%), Query Frame = -1

Query:  117 SSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKT----PNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFM----------LEEGSGSTDLQKTYCERKQLSTESA 515
            + RRLMR+ + +  ++ P +  S   +N+  W+ ++ G  +T YE+G +  ++ F   YP K P ++  +    PN     N  IC     NR W+  Y    I+ SI S            +E  +   D +  Y +R + + E +
Sbjct:    5 ARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPN--VYANGEICLDILQNR-WTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKS 148

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BLAST of SMED30032966 vs. Ensembl Nematostella
Match: EDO38458 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCT8])

HSP 1 Score: 94.7449 bits (234), Expect = 2.551e-24
Identity = 52/159 (32.70%), Postives = 82/159 (51.57%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +    ++ RL ++   I  + VPYI  +   +NI EWH+V+ G  NT YE GFY+G ++FP ++P K P I + TPNGRF VNT     N                L +++F  E       ++ +  +++Q +  S  +NLQ   F E FP I ++I+
Sbjct:    6 RIHTTAAARLRQDYLRIVRDPVPYITAAPLPSNILEWHYVVRGPENTPYEGGFYHGKLVFPRDFPFKPPSIYMITPNGRFKVNTRYYTANTH--------------LELFTFQNETAPTLGSVETSDFQKRQYAVMSGPFNLQDRIFCELFPKIAEEIR 150

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BLAST of SMED30032966 vs. Ensembl Nematostella
Match: EDO38825 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBT3])

HSP 1 Score: 88.1965 bits (217), Expect = 8.376e-23
Identity = 45/129 (34.88%), Postives = 72/129 (55.81%), Query Frame = -1

Query:  123 MREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNR--SWSSVYSPLYIMLSIYSFMLEEGSGSTD-LQKTYCERKQLSTE 500
            MREA+E+  N+    H     +N++EWHF + G P+T +  G Y+G +I P  YP+K P I + TPNGRF +   IC    ++   +W   +S   ++++I  FM  +G+G+   L  T  ER  L+ +
Sbjct:    1 MREAKEL-RNATELYHAQPLEDNLFEWHFTVRGPPDTEFAGGRYHGRIILPPEYPMKPPSIMLLTPNGRFEIGKKICLSMSAHHPETWQPSWSIRTVLMAIIGFMPTKGAGAIGALDYTPKERAVLARK 128

[Close]

BLAST of SMED30032966 vs. Ensembl Nematostella
Match: EDO28988 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4X5])

HSP 1 Score: 68.1662 bits (165), Expect = 1.249e-15
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = -1

Query:  237 NNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNR------SWSS 437
            +N++EWHF + G P+T +  G Y+G +I P  YP+K P I + TPNGRF +   IC    ++       SWSS
Sbjct:    2 DNLFEWHFTVRGPPDTEFAGGRYHGRIILPPEYPMKPPSIMLLTPNGRFEIGKKICLSMSAHHPETWQPSWSS 74

[Close]

BLAST of SMED30032966 vs. Ensembl Nematostella
Match: EDO33976 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQR6])

HSP 1 Score: 62.003 bits (149), Expect = 2.853e-12
Identity = 32/118 (27.12%), Postives = 61/118 (51.69%), Query Frame = -1

Query:  186 TSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 536
            T+K +  +++R+ RE  EI  +  P        +++YEW+  + G P + YE G ++  + FP +YP K P++  +T      +N+  +  L+    SWS   +   ++LSI S + +
Sbjct:   38 TAKNQSTAAKRIQRELTEITLDPPPNCSAGPKGDDLYEWYSTILGPPGSVYEGGVFFLDIHFPSDYPFKPPKVTFRTRIYHCNINSQGMVCLDILKDSWSPALTISKVLLSICSLLTD 155

[Close]

BLAST of SMED30032966 vs. Ensembl Nematostella
Match: EDO33975 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQR5])

HSP 1 Score: 57.7658 bits (138), Expect = 9.399e-11
Identity = 32/113 (28.32%), Postives = 57/113 (50.44%), Query Frame = -1

Query:  186 VSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT--NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 518
             +++R+ RE   I  +  P        + +YEW+  + G P + YE G ++  + FP +YP K P+I  +T      +N+  +IC L+    SWS   +   ++LSI S + +
Sbjct:   39 TAAKRIQRELAGITLDPPPNCSAGPKGDKLYEWYSTILGPPGSVYEGGVFFLDIHFPTDYPFKPPKITFRTRIYHCNINSQGHIC-LDILKDSWSPALTMSKVLLSICSLLTD 150

[Close]

BLAST of SMED30032966 vs. Ensembl Medaka
Match: ube2j2 (ubiquitin conjugating enzyme E2 J2 [Source:NCBI gene;Acc:101164303])

HSP 1 Score: 114.005 bits (284), Expect = 7.192e-31
Identity = 57/161 (35.40%), Postives = 96/161 (59.63%), Query Frame = -1

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QLS +S  +NL+   F E FP+++ +I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDYTKRQLSAQSLAFNLKDKVFCELFPDVVDEIK 168

[Close]

Query:   54 KAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSN-RSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQ 530
            +A   +++RL ++   I  + VPYI      +NI EWH+V+ G   T YE G+Y+G +IFP  +P K P I + TPNGRF  NT +C ++ D +  +W+  +S   I+  + SFM+E+G     ++ +   ++QLS +S  +NL+   F E FP+++ +I+
Sbjct:    8 RAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDYTKRQLSAQSLAFNLKDKVFCELFPDVVDEIK 168

[Close]

BLAST of SMED30032966 vs. Ensembl Medaka
Match: ube2j1 (ubiquitin conjugating enzyme E2 J1 [Source:NCBI gene;Acc:101160616])

HSP 1 Score: 92.8189 bits (229), Expect = 1.763e-22
Identity = 49/137 (35.77%), Postives = 79/137 (57.66%), Query Frame = -1

Query:  111 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSN---RSWSSVYSPLYIMLSIYSFMLEEGSGS-TDLQKTYCERKQLSTESANY 509
            +RLM+EA E+  +   + H    ++N++EWHF + G P++ ++ G Y+G +I P  YP+K P I + TPNGRF V   IC L+ S     +W   +S    +++I  FM  +G G+   L  T  ERK L+ +S ++
Sbjct:   13 KRLMKEAAEL-RDPTEHYHAQPLDDNLFEWHFSVRGPPDSDFDGGVYHGRIILPPEYPMKPPSIILLTPNGRFEVGKKIC-LSISGHHPETWQPSWSIRTALIAIIGFMPTKGEGAIGSLDYTPEERKALAKKSQDF 147

[Close]

BLAST of SMED30032966 vs. Ensembl Medaka
Match: ube2j1 (ubiquitin conjugating enzyme E2 J1 [Source:NCBI gene;Acc:101160616])

HSP 1 Score: 80.1073 bits (196), Expect = 9.247e-18
Identity = 33/80 (41.25%), Postives = 51/80 (63.75%), Query Frame = -1

Query:  270 RRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC 509
            +RLM+EA E+  +   + H    ++N++EWHF + G P++ ++ G Y+G +I P  YP+K P I + TPNGRF V   IC
Sbjct:   13 KRLMKEAAEL-RDPTEHYHAQPLDDNLFEWHFSVRGPPDSDFDGGVYHGRIILPPEYPMKPPSIILLTPNGRFEVGKKIC 91

[Close]

BLAST of SMED30032966 vs. Ensembl Medaka
Match: ENSORLT00000020581.2 (ubiquitin-conjugating enzyme E2 E1 [Source:NCBI gene;Acc:101156678])

HSP 1 Score: 58.9214 bits (141), Expect = 9.946e-11
Identity = 33/124 (26.61%), Postives = 60/124 (48.39%), Query Frame = -1

Query:  186 KLVFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNT-NICALNDSNRSWSSVYSPLYIMLSIYSFMLE 554
            K+    TSK    S++R+ +E  +I  +  P        +NIYEW   + G P + YE G ++  + F  +YP K P++  +T      +N+  +  L+    +WS   +   ++LSI S + +
Sbjct:   36 KVSMSKTSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTD 159

[Close]

BLAST of SMED30032966 vs. Planmine SMEST
Match: SMESG000074194.1 (SMESG000074194.1)

HSP 1 Score: 286.189 bits (731), Expect = 1.865e-99
Identity = 146/181 (80.66%), Postives = 148/181 (81.77%), Query Frame = -1

Query:    6 VFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNRSWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQMLISLAKMNFI 548
            +FFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY              EIRIKTPNGRFLVNTNICALNDSNRSW                  +EGSGSTDLQK YCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQMLISLAKMNFI
Sbjct:   42 LFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY--------------EIRIKTPNGRFLVNTNICALNDSNRSW------------------KEGSGSTDLQKIYCERKQLSTESANYNLQIPEFREYFPNILQKIQSTDYQMLISLAKMNFI 190

[Close]

BLAST of SMED30032966 vs. Planmine SMEST
Match: SMESG000074194.1 (SMESG000074194.1)

HSP 1 Score: 179.104 bits (453), Expect = 7.355e-68
Identity = 89/104 (85.58%), Postives = 90/104 (86.54%), Query Frame = -1

Query:  237 VFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNRSWSS 548
            +FFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY              EIRIKTPNGRFLVNTNICALNDSNRSWSS
Sbjct:   42 LFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY--------------EIRIKTPNGRFLVNTNICALNDSNRSWSS 131

HSP 2 Score: 96.2857 bits (238), Expect = 7.355e-68
Identity = 48/51 (94.12%), Postives = 48/51 (94.12%), Query Frame = -2

Query:   35 KKGQVRLIYRKPIANVNSCPPRVQIIIYKFQNSENIFQTFCRKSNQPIIKC 187
            KK QVRLIYRK IANVNSCPPRVQIIIYKFQNSENIFQ FCRKSNQPIIKC
Sbjct:  132 KKVQVRLIYRKSIANVNSCPPRVQIIIYKFQNSENIFQIFCRKSNQPIIKC 182

[Close]

BLAST of SMED30032966 vs. Planmine SMEST
Match: SMESG000074194.1 (SMESG000074194.1)

HSP 1 Score: 129.028 bits (323), Expect = 1.346e-38
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = -1

Query:  360 VFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGI 548
            +FFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY +
Sbjct:   42 LFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYEL 104

[Close]

BLAST of SMED30032966 vs. Planmine SMEST
Match: SMESG000074194.1 (SMESG000074194.1)

HSP 1 Score: 130.568 bits (327), Expect = 4.100e-38
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = -1

Query:  360 VFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGI 548
            +FFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYY +
Sbjct:  120 LFFMTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYEL 182

[Close]

BLAST of SMED30032966 vs. Planmine SMEST
Match: SMESG000064580.1 (SMESG000064580.1)

HSP 1 Score: 97.4413 bits (241), Expect = 7.431e-25
Identity = 51/141 (36.17%), Postives = 84/141 (59.57%), Query Frame = -1

Query:   51 VPYIHFSIANNNIYEWHFVLEGAPNTAYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNIC-ALNDSNR-SWSSVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEFREYFPNILQKIQS 467
            +P+I     ++NI+EWH+++EG  ++ Y  GFY+G + F   YP K+P I + TPNGRF   T +C +++D ++ SW+  +    I+  + SFMLEE      +  +   R QL+ ES  YN++   F E FP  ++KI++
Sbjct:   21 IPFIIAEPLSSNIFEWHYIIEGPKDSDYYGGFYHGYLKFSQEYPFKAPSIYMITPNGRFQCRTRLCLSISDFHQESWNPSWCVGTILTGLLSFMLEESPTFGSISSSKETRWQLARESLEYNIKDFVFAELFPETVEKIRA 161

The following BLAST results are available for this feature:

BLAST of SMED30032966 vs. Ensembl Human
BLAST of SMED30032966 vs. Ensembl Celegans
BLAST of SMED30032966 vs. Ensembl Fly
BLAST of SMED30032966 vs. Ensembl Zebrafish
BLAST of SMED30032966 vs. Ensembl Xenopus
BLAST of SMED30032966 vs. Ensembl Mouse
BLAST of SMED30032966 vs. UniProt/SwissProt
BLAST of SMED30032966 vs. TrEMBL
BLAST of SMED30032966 vs. Ensembl Cavefish
BLAST of SMED30032966 vs. Ensembl Sea Lamprey
BLAST of SMED30032966 vs. Ensembl Yeast
BLAST of SMED30032966 vs. Ensembl Nematostella
BLAST of SMED30032966 vs. Ensembl Medaka
BLAST of SMED30032966 vs. Planmine SMEST

BLAST of SMED30032966 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
UBE2J2	8.861e-31	34.36	ubiquitin conjugating enzyme E2 J2 [Source:HGNC Sy...	[more]
UBE2J2	4.365e-30	34.36	ubiquitin conjugating enzyme E2 J2 [Source:HGNC Sy...	[more]
UBE2J2	4.365e-30	34.36	ubiquitin conjugating enzyme E2 J2 [Source:HGNC Sy...	[more]
UBE2J2	6.898e-26	31.28	ubiquitin conjugating enzyme E2 J2 [Source:HGNC Sy...	[more]
UBE2J1	9.801e-21	34.31	ubiquitin conjugating enzyme E2 J1 [Source:HGNC Sy...	[more]

BLAST of SMED30032966 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ubc-26	6.437e-28	32.35	UBiquitin Conjugating enzyme [Source:UniProtKB/Tr...	[more]
ubc-26	6.437e-28	32.35	UBiquitin Conjugating enzyme [Source:UniProtKB/Tr...	[more]
ubc-26	1.344e-27	32.35	UBiquitin Conjugating enzyme [Source:UniProtKB/Tr...	[more]
ubc-26	1.344e-27	32.35	UBiquitin Conjugating enzyme [Source:UniProtKB/Tr...	[more]
ubc-15	2.932e-25	30.43	UBiquitin Conjugating enzyme [Source:UniProtKB/Tr...	[more]

BLAST of SMED30032966 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG5823	2.725e-23	29.01	gene:FBgn0038515 transcript:FBtr0321266	[more]
CG5823	2.725e-23	29.01	gene:FBgn0038515 transcript:FBtr0083505	[more]
Ubc87F	6.275e-10	27.03	gene:FBgn0267383 transcript:FBtr0082838	[more]
Ubc2	9.969e-9	26.50	gene:FBgn0015320 transcript:FBtr0080115	[more]
Ubc2	9.969e-9	26.50	gene:FBgn0015320 transcript:FBtr0345437	[more]

BLAST of SMED30032966 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ube2j2	3.547e-30	32.93	ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog,...	[more]
ube2j1	3.561e-22	35.04	ubiquitin-conjugating enzyme E2, J1 [Source:ZFIN;A...	[more]
ube2j2	4.286e-20	36.79	ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog,...	[more]
ube2e1	2.742e-10	27.12	ubiquitin-conjugating enzyme E2E 1 [Source:ZFIN;Ac...	[more]
ube2e2	6.372e-10	26.79	ubiquitin-conjugating enzyme E2E 2 [Source:ZFIN;Ac...	[more]

BLAST of SMED30032966 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ube2j1	2.273e-21	35.77	ubiquitin conjugating enzyme E2 J1 [Source:NCBI ge...	[more]
ube2j2	7.766e-20	34.78	ubiquitin conjugating enzyme E2 J2 [Source:NCBI ge...	[more]
ube2g2	7.466e-11	27.27	ubiquitin conjugating enzyme E2 G2 [Source:NCBI ge...	[more]
nog2	8.662e-10	27.42	noggin 2 [Source:Xenbase;Acc:XB-GENE-487602]	[more]
cox5a	9.643e-10	26.79	cytochrome c oxidase subunit Va [Source:Xenbase;Ac...	[more]

BLAST of SMED30032966 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ube2j2	4.123e-30	35.40	ubiquitin-conjugating enzyme E2J 2 [Source:MGI Sym...	[more]
Ube2j2	4.123e-30	35.40	ubiquitin-conjugating enzyme E2J 2 [Source:MGI Sym...	[more]
Ube2j2	4.123e-30	35.40	ubiquitin-conjugating enzyme E2J 2 [Source:MGI Sym...	[more]
Ube2j2	5.114e-30	35.40	ubiquitin-conjugating enzyme E2J 2 [Source:MGI Sym...	[more]
Ube2j2	5.114e-30	35.40	ubiquitin-conjugating enzyme E2J 2 [Source:MGI Sym...	[more]

BLAST of SMED30032966 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9SHI7\|UBC34_ARATH	4.635e-31	35.44	Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis ...	[more]
sp\|Q8N2K1\|UB2J2_HUMAN	2.096e-29	34.36	Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens...	[more]
sp\|Q6P073\|UB2J2_MOUSE	2.884e-29	35.40	Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus...	[more]
sp\|Q2TA03\|UB2J2_BOVIN	4.856e-29	34.78	Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus O...	[more]
sp\|Q9FK29\|UBC33_ARATH	1.930e-28	34.18	Probable ubiquitin-conjugating enzyme E2 33 OS=Ara...	[more]

BLAST of SMED30032966 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1Y1HJC5	1.962e-31	38.82	Ubiquitin-conjugating enzyme OS=Klebsormidium nite...	[more]
A0A0K9S3W6	6.911e-31	36.71	UBIQUITIN_CONJUGAT_2 domain-containing protein OS=...	[more]
A0A0R3TKV8	1.261e-30	34.81	UBIQUITIN_CONJUGAT_2 domain-containing protein OS=...	[more]
A0A383WB10	1.669e-30	39.74	UBIQUITIN_CONJUGAT_2 domain-containing protein OS=...	[more]
A8JEV0	2.503e-30	36.18	Predicted protein (Fragment) OS=Chlamydomonas rein...	[more]

BLAST of SMED30032966 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ube2j2	6.606e-30	32.93	ubiquitin conjugating enzyme E2 J2 [Source:NCBI ge...	[more]
ube2j2	3.810e-23	33.33	ubiquitin conjugating enzyme E2 J2 [Source:NCBI ge...	[more]
ube2j1	3.322e-21	32.14	ubiquitin conjugating enzyme E2 J1 [Source:NCBI ge...	[more]
ube2e1	9.479e-11	26.32	ubiquitin-conjugating enzyme E2E 1 [Source:ZFIN;Ac...	[more]
ube2e3	3.887e-10	27.03	ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog...	[more]

BLAST of SMED30032966 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ube2g2	9.071e-10	25.62	ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, ...	[more]
ENSPMAT00000008817.1	1.096e-6	28.57	pep scaffold:Pmarinus_7.0:GL476329:1748443:1760143...	[more]
ENSPMAT00000010378.1	1.754e-6	24.56	pep scaffold:Pmarinus_7.0:GL477318:26908:31295:-1 ...	[more]

BLAST of SMED30032966 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
UBC6	1.114e-25	33.55	Ubiquitin-conjugating enzyme involved in ERAD; loc...	[more]
UBC7	3.345e-11	26.98	Ubiquitin conjugating enzyme; involved in the ER-a...	[more]
UBC13	1.137e-8	26.36	E2 ubiquitin-conjugating enzyme; involved in the e...	[more]
UBC4	5.095e-8	26.32	Ubiquitin-conjugating enzyme (E2); key E2 partner ...	[more]
RAD6	9.098e-8	25.17	Ubiquitin-conjugating enzyme (E2); involved in pos...	[more]

BLAST of SMED30032966 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO38458	2.551e-24	32.70	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38825	8.376e-23	34.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO28988	1.249e-15	41.10	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33976	2.853e-12	27.12	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33975	9.399e-11	28.32	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30032966 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ube2j2	7.192e-31	35.40	ubiquitin conjugating enzyme E2 J2 [Source:NCBI ge...	[more]
ube2j2	7.192e-31	35.40	ubiquitin conjugating enzyme E2 J2 [Source:NCBI ge...	[more]
ube2j1	1.763e-22	35.77	ubiquitin conjugating enzyme E2 J1 [Source:NCBI ge...	[more]
ube2j1	9.247e-18	41.25	ubiquitin conjugating enzyme E2 J1 [Source:NCBI ge...	[more]
ENSORLT00000020581.2	9.946e-11	26.61	ubiquitin-conjugating enzyme E2 E1 [Source:NCBI ge...	[more]

BLAST of SMED30032966 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000074194.1	1.865e-99	80.66	SMESG000074194.1	[more]
SMESG000074194.1	7.355e-68	85.58	SMESG000074194.1	[more]
SMESG000074194.1	1.346e-38	95.24	SMESG000074194.1	[more]
SMESG000074194.1	4.100e-38	95.24	SMESG000074194.1	[more]
SMESG000064580.1	7.431e-25	36.17	SMESG000064580.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30032966 ID=SMED30032966|Name=SMED30032966|organism=Schmidtea mediterranea sexual|type=transcript|length=584bp
GCTTAGATGAAATTCATTTTTGCTAAGGAAATCAACATTTGATAATCGGT
TGATTGGATTTTCTGCAAAATGTTTGGAAAATATTCTCGGAATTCTGGAA
TTTGTAAATTATAATTTGCACTCTCGGTGGACAGCTGTTTACGTTCGCAA
TAGGTTTTCTGTAGATCAGTCGAACCTGACCCTTCTTCTAGCATGAAAGA
GTAAATACTTAACATTATATACAAAGGAGAATAAACTGAACTCCATGACC
TATTGCTGTCATTTAATGCACAAATATTTGTGTTGACCAAAAATCTTCCA
TTTGGTGTTTTAATTCTAATTTCTGGACTTTTAATTGGATAATTCAAAGG
AAAAATCATGATACCGTAGTAAAATCCGTTTTCATATGCCGTATTCGGAG
CACCTTCGAGGACAAAATGCCATTCATAAATATTATTATTAGCAATTGAA
AAATGTATATAAGGAACGGAATTTGTACATATTTCTTCAGCTTCTCTCAT
AAGCCTTCTACTGCTAACAATTTCTGCTTTACTTGTCATGAAAAAAACTA
ATTTTAAAACAAAACTTTATTTAATTTAGAAATG

protein sequence of SMED30032966-orf-1

>SMED30032966-orf-1 ID=SMED30032966-orf-1|Name=SMED30032966-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=179bp
MTSKAEIVSSRRLMREAEEICTNSVPYIHFSIANNNIYEWHFVLEGAPNT
AYENGFYYGIMIFPLNYPIKSPEIRIKTPNGRFLVNTNICALNDSNRSWS
SVYSPLYIMLSIYSFMLEEGSGSTDLQKTYCERKQLSTESANYNLQIPEF
REYFPNILQKIQSTDYQMLISLAKMNFI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000608	UBQ-conjugat_E2
IPR016135	UBQ-conjugating_enzyme/RWD

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 11..156 e-value: 2.7E-14 score: 63.5
None	No IPR available	PANTHER	PTHR24067:SF257	UBIQUITIN CONJUGATING ENZYME	coord: 8..159
None	No IPR available	PANTHER	PTHR24067	UBIQUITIN-CONJUGATING ENZYME E2	coord: 8..159
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 12..119 e-value: 4.5E-14 score: 52.3
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 11..118 score: 16.622
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 10..118 e-value: 2.77566E-31 score: 108.444
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	G3DSA:3.10.110.10		coord: 1..173 e-value: 2.2E-41 score: 143.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	SSF54495	UBC-like	coord: 10..130