Omega-amidase NIT2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032965 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Match: NIT2 (nitrilase family member 2 [Source:HGNC Symbol;Acc:HGNC:29878]) HSP 1 Score: 258.07 bits (658), Expect = 8.312e-85 Identity = 124/275 (45.09%), Postives = 186/275 (67.64%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
++ALIQ +SS K+ N+++ S I+ A G K+V LPE FN P+G++ + +E IP GE+ Q LS+V+KEC+I+++GGSIPE A K+YN+ +F P+G L+ Y K+HLFD + P ++ES+T PG+ TF+ P C+ VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS +PWG+V+ +E I+ +DL + E+R IP+ +Q+R D+Y+V
Sbjct: 4 FRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIP-GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCR-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAV 271
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Match: NIT2 (nitrilase family member 2 [Source:HGNC Symbol;Acc:HGNC:29878]) HSP 1 Score: 154.066 bits (388), Expect = 1.610e-44 Identity = 76/174 (43.68%), Postives = 116/174 (66.67%), Query Frame = 2
Query: 71 IQMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRN 583
+ ++ALIQ +SS K+ N+++ S I+ A G K+V LPE FN P+G++ + +E IP GE+ Q LS+V+KEC+I+++GGSIPE A K+YN+ +F P+G L+ Y K+HLFD + P ++ES+T PG+ TF+ P C+ VG+GIC+D+RF E+A IY +
Sbjct: 95 LHAFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIP-GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCR-----VGLGICYDMRFAELAQIYAQR 262
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Match: NIT1 (nitrilase 1 [Source:HGNC Symbol;Acc:HGNC:7828]) HSP 1 Score: 146.362 bits (368), Expect = 2.793e-41 Identity = 88/280 (31.43%), Postives = 142/280 (50.71%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSED---LLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYK---ESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
VA+ Q + K N ++ A + G L LPE F+ F + D L SE + G+ + +++++EC +W+ G E + KIYN ++ N G ++ TY K HL D P ES +++PG L + + P K +G+ +C+D+RFPE++ + +L YP+AF TGPA+WE+L R A++ QCYV + + SYGHS+ DPWG V+ E G+ +DLN + ++R +P+ + R D+Y
Sbjct: 13 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFD--FIARDPAETLHLSEPL-GGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGK-----IGLAVCYDMRFPELS-LALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 283
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Match: NIT1 (nitrilase 1 [Source:HGNC Symbol;Acc:HGNC:7828]) HSP 1 Score: 146.747 bits (369), Expect = 3.569e-41 Identity = 88/280 (31.43%), Postives = 142/280 (50.71%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSED---LLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYK---ESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
VA+ Q + K N ++ A + G L LPE F+ F + D L SE + G+ + +++++EC +W+ G E + KIYN ++ N G ++ TY K HL D P ES +++PG L + + P K +G+ +C+D+RFPE++ + +L YP+AF TGPA+WE+L R A++ QCYV + + SYGHS+ DPWG V+ E G+ +DLN + ++R +P+ + R D+Y
Sbjct: 34 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFD--FIARDPAETLHLSEPL-GGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGK-----IGLAVCYDMRFPELS-LALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 304
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Match: NIT1 (nitrilase 1 [Source:HGNC Symbol;Acc:HGNC:7828]) HSP 1 Score: 146.747 bits (369), Expect = 5.630e-41 Identity = 88/280 (31.43%), Postives = 142/280 (50.71%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSED---LLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYK---ESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
VA+ Q + K N ++ A + G L LPE F+ F + D L SE + G+ + +++++EC +W+ G E + KIYN ++ N G ++ TY K HL D P ES +++PG L + + P K +G+ +C+D+RFPE++ + +L YP+AF TGPA+WE+L R A++ QCYV + + SYGHS+ DPWG V+ E G+ +DLN + ++R +P+ + R D+Y
Sbjct: 49 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFD--FIARDPAETLHLSEPL-GGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGK-----IGLAVCYDMRFPELS-LALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 319
BLAST of Omega-amidase NIT2 vs. Ensembl Celegans
Match: nft-1 (Nitrilase and fragile histidine triad fusion protein NitFhit Bis(5'-adenosyl)-triphosphatase Nitrilase homolog [Source:UniProtKB/Swiss-Prot;Acc:O76463]) HSP 1 Score: 131.339 bits (329), Expect = 8.301e-35 Identity = 86/288 (29.86%), Postives = 146/288 (50.69%), Query Frame = 2
Query: 86 VALIQNIVSSCKAT-------NLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENI-----PNGETYQMLSKVSKECAIWIVGGSIPELR---ANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+A ++ ++ C+ T N ++ I+ A + ++V LPE F+ G L ++E I + E + +++++ IW+ G + A +N+ +I + +G Y+K+HLFD P ESE S G E+ IP +G+ IC+D+RFPE++ ++ R + LL +P+AF + TG A+WE L R A++NQCYV + + SYGHS+ DPWG V+ E+ + +DL+ VD +R M P+ R D+Y++
Sbjct: 10 MATGRHFIAVCQMTSDNDLEKNFQAAKNMIERAGEKKCEMVFLPECFDF-IG----LNKNEQIDLAMATDCEYMEKYRELARKHNIWLSLGGLHHKDPSDAAHPWNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEM----IPPVDTPIGRLGLSICYDVRFPELS-LWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAHNPKRQSYGHSMVVDPWGAVVAQCSERVDMCFAEIDLSYVDTLREMQPVFSHRRSDLYTL 287
BLAST of Omega-amidase NIT2 vs. Ensembl Celegans
Match: upb-1 (UreidoPropionase Beta [Source:UniProtKB/TrEMBL;Acc:Q19437]) HSP 1 Score: 64.3142 bits (155), Expect = 1.962e-11 Identity = 74/309 (23.95%), Postives = 132/309 (42.72%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSCKATNLSQVESK----------IKLATKSGVKLVCLPEYFNCPFG---SEDL--LKQSENIPNGETYQMLSKVSKECAIWIVGGSIP--ELRANKIYNSSMIFNPNGQLIGTYSKMHL---FDSNRPIYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCY-VGLCSIARDY----------EAYHISYGH----SLATDPWGKVICSLDE-KEGILEFSVDLNVVDEVRSMIPIRKQERDDVYS 892
++++VA IQN + + T S VE + I+ A +G ++ L E + PF E L + +E++ G T Q LSK++ + I I+ + E + + I+N++++ + G++IG K H+ D N Y T P+ + K +GI IC+ P+ +Y N ++ P+A A W + R A+ N + VG+ + + + H +GH S P G +L +EG+L +DLN+ + + R R D+Y+
Sbjct: 72 RLVRVAAIQNKIH--RPTTDSVVEQRDAIHQRVGAMIEAAASAGANVIGLQEAWTMPFAFCTRERLPWTEFAESVYTGPTTQFLSKLAVKHDIVIISPILERDEEKDDVIWNTAVVISHTGRVIGRSRKNHIPRVGDFNESTYYMEST---------LGHPVFETKYGRIGINICYGRHHPQNWMMYALNG-AEIIFNPSATVGALSEPLWGIEARNAAIANHVFTVGINRVGTEVFPNEFTSGNGQPAHKDFGHFYGSSYIAAPDGSRTPALSRVREGVLIAELDLNLCRQCKDAWGFRMTNRLDMYA 368
BLAST of Omega-amidase NIT2 vs. Ensembl Fly
Match: CG8132 (gene:FBgn0037687 transcript:FBtr0082029) HSP 1 Score: 224.172 bits (570), Expect = 8.231e-72 Identity = 118/278 (42.45%), Postives = 173/278 (62.23%), Query Frame = 2
Query: 77 MLKVALIQNIVSSCKATNLSQVESKIKLATKSGV-KLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANK-IYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
++++AL+Q S K N+ +KI+ A K +L+ LPE FN P+G++ + SE IP+G T Q LS ++++ ++IVGG+IPEL N IYN+ +++P G L+ + KMHLFD + +KESET GN+ N+ K +GIGIC+D+RF E+A +Y RN C +++YPAAFNM TGP +WELL R A DNQ +V S ARD A +++YGHS+ +PW KV S E E I+ +D + V++VR IP+ Q R D+Y+
Sbjct: 7 IMRLALLQLKGSKDKVANVQNAVTKIEAAVKEHKPRLITLPECFNAPYGTKYFREYSETIPDGYTSQQLSNLARKHQVYIVGGTIPELGENDAIYNTCTVWSPTGDLVAKHRKMHLFDIDVKGGIRFKESETLSAGNDFTIINVDGHK-----IGIGICYDIRFEEMARLY-RNAGCEMIIYPAAFNMTTGPLHWELLQRSRANDNQLFVVTTSPARDTSAEYVAYGHSMVVNPWAKVQQSASEGEEIVVADIDFSEVEQVRQQIPVFGQRRLDLYAT 278
BLAST of Omega-amidase NIT2 vs. Ensembl Fly
Match: NitFhit (gene:FBgn0024945 transcript:FBtr0072483) HSP 1 Score: 145.206 bits (365), Expect = 1.333e-39 Identity = 95/286 (33.22%), Postives = 147/286 (51.40%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSE---DLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFD--SNRPIYKESETSVPGNELFTFNIPLCKEKNIT-----VGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKE-GILEFSVDLNVVDEVRSMIPIRKQERDDVYSVT 898
Q +A+ Q +S KA NLSQV + A ++ LPE C F E ++ SE + +GE +++K IWI G + E KI+N+ ++ N G+L Y K+H+FD + +ES+T PG C E+ ++ +G+ IC+DLRF E A + R NLL YP+AF ATG A+WE+L R A++ QC+V + + S+GHS+ PWG V+ E+E I VDL+V+ + +P + R+D+Y++T
Sbjct: 31 QSATIAVGQMRSTSDKAANLSQVIELVDRAKSQNACMLFLPEC--CDFVGESRTQTIELSEGL-DGELMAQYRELAKCNKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTKEVRLRESDTVTPG---------YCLERPVSTPVGQIGLQICYDLRFAEPA-VLLRKLGANLLTYPSAFTYATGKAHWEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEVDLSVLQSLYQTMPCFEHRRNDIYALT 303
BLAST of Omega-amidase NIT2 vs. Ensembl Fly
Match: pyd3 (gene:FBgn0037513 transcript:FBtr0081780) HSP 1 Score: 56.6102 bits (135), Expect = 8.974e-9 Identity = 67/305 (21.97%), Postives = 131/305 (42.95%), Query Frame = 2
Query: 74 QMLKVALIQNIV--------SSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFG---SEDL--LKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL---RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCY------VGLCSIARDYEA---------YHISYGHSLATDPWGKVICSLD-EKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYS 892
++++V IQN + + ++V++ IK A ++G +VC E + PF E + +E NG T +ML++++K + I+ SI E I+N++++ + +G+ +G + K H+ + ES + GN P+ + + + + IC+ P+ ++ N ++ P+A W + R A+ N + VG +Y + + YG S P G SL +K+G+L +DLN+ +V+ R +R +Y+
Sbjct: 71 RIVRVGAIQNSIVIPTTAPIEKQREAIWNKVKTMIKAAAEAGCNIVCTQEAWTMPFAFCTREKFPWCEFAEEAENGPTTKMLAELAKAYNMVII-HSILERDMEHGETIWNTAVVISNSGRYLGKHRKNHIPRVGD--FNESTYYMEGNT----GHPVFETEFGKLAVNICYGRHHPQNWMMFGLN-GAEIVFNPSATIGRLSEPLWSIEARNAAIANSYFTVPINRVGTEQFPNEYTSGDGNKAHKEFGPFYGSSYVAAPDGSRTPSLSRDKDGLLVVELDLNLCRQVKDFWGFRMTQRVPLYA 367
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Match: nit2 (nitrilase family, member 2 [Source:NCBI gene;Acc:402904]) HSP 1 Score: 259.225 bits (661), Expect = 2.069e-85 Identity = 128/278 (46.04%), Postives = 183/278 (65.83%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q VS KA NL + ++ + A G K+V LPE FN P+G+ + +E IP GE+ Q+LS+ +K+C I++VGGSIPE K+YN+ +F P+G+L+ T+ K+HLFD + P ++ESET PG L F P CK VG+GIC+D+RF E+A IY + K C LL+YP AFNM TGPA+WELL RG A+DNQ YV S ARD A ++++GHS +PWG+VI +E ++ +DL + +VR IPI KQ R+D+YSV ++
Sbjct: 11 FRLAVVQLHVSKIKADNLGRAQTLVTEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKIP-GESTQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGKLLVTHRKIHLFDIDVPGKIRFQESETLSPGKSLSMFETPYCK-----VGVGICYDIRFAELAQIYAK-KGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEESVVYADIDLQYLADVRQQIPITKQRRNDLYSVNSV 281
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Match: nit1 (nitrilase 1 [Source:ZFIN;Acc:ZDB-GENE-040912-65]) HSP 1 Score: 149.443 bits (376), Expect = 2.963e-42 Identity = 91/280 (32.50%), Postives = 151/280 (53.93%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-----KIYNSSMIFNPNGQLIGTYSKMHLFD---SNRPI-YKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSL-DEKEGILEFSVDLNVVDEVRSMIPIRKQERD-DVY 889
A+ Q S K N ++ A G +V LPE F+ S E+ L+ SE++ +GET + ++++ +W+ G E + +IYNS +I N G+++ Y K HLFD S++ + KES ++PG L +P + VG+G+C+DLRFPE++ R+ +L YP+AF +ATG A+WE+L R A++ QC+V + + + SYGH+LA DPWG+V+ +EG+ ++L + ++R +P+ + R D Y
Sbjct: 38 AAVCQMTASPDKEANFRTCTRLVEQAKVGGASMVFLPEGFDYIGSSREETLQLSESL-DGETISRYTHLARKLDVWLSLGGFHEQGHDWKTDRRIYNSHIIINGQGEIVSVYRKTHLFDVELSSKGVSLKESAFTIPGPRL----VPPVQTPIGKVGLGVCYDLRFPELSAALQRHG-AEILTYPSAFTVATGTAHWEVLLRARAVETQCFVLAAAQVGAHHSKRASYGHALAVDPWGEVLGDCGGTQEGVTLAHINLQKLRDIRRDMPVLQHRRQTDFY 311
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Match: nit1 (nitrilase 1 [Source:ZFIN;Acc:ZDB-GENE-040912-65]) HSP 1 Score: 149.058 bits (375), Expect = 3.382e-42 Identity = 91/280 (32.50%), Postives = 151/280 (53.93%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-----KIYNSSMIFNPNGQLIGTYSKMHLFD---SNRPI-YKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSL-DEKEGILEFSVDLNVVDEVRSMIPIRKQERD-DVY 889
A+ Q S K N ++ A G +V LPE F+ S E+ L+ SE++ +GET + ++++ +W+ G E + +IYNS +I N G+++ Y K HLFD S++ + KES ++PG L +P + VG+G+C+DLRFPE++ R+ +L YP+AF +ATG A+WE+L R A++ QC+V + + + SYGH+LA DPWG+V+ +EG+ ++L + ++R +P+ + R D Y
Sbjct: 36 AAVCQMTASPDKEANFRTCTRLVEQAKVGGASMVFLPEGFDYIGSSREETLQLSESL-DGETISRYTHLARKLDVWLSLGGFHEQGHDWKTDRRIYNSHIIINGQGEIVSVYRKTHLFDVELSSKGVSLKESAFTIPGPRL----VPPVQTPIGKVGLGVCYDLRFPELSAALQRHG-AEILTYPSAFTVATGTAHWEVLLRARAVETQCFVLAAAQVGAHHSKRASYGHALAVDPWGEVLGDCGGTQEGVTLAHINLQKLRDIRRDMPVLQHRRQTDFY 309
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Match: nit1 (nitrilase 1 [Source:ZFIN;Acc:ZDB-GENE-040912-65]) HSP 1 Score: 149.058 bits (375), Expect = 3.382e-42 Identity = 91/280 (32.50%), Postives = 151/280 (53.93%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-----KIYNSSMIFNPNGQLIGTYSKMHLFD---SNRPI-YKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSL-DEKEGILEFSVDLNVVDEVRSMIPIRKQERD-DVY 889
A+ Q S K N ++ A G +V LPE F+ S E+ L+ SE++ +GET + ++++ +W+ G E + +IYNS +I N G+++ Y K HLFD S++ + KES ++PG L +P + VG+G+C+DLRFPE++ R+ +L YP+AF +ATG A+WE+L R A++ QC+V + + + SYGH+LA DPWG+V+ +EG+ ++L + ++R +P+ + R D Y
Sbjct: 36 AAVCQMTASPDKEANFRTCTRLVEQAKVGGASMVFLPEGFDYIGSSREETLQLSESL-DGETISRYTHLARKLDVWLSLGGFHEQGHDWKTDRRIYNSHIIINGQGEIVSVYRKTHLFDVELSSKGVSLKESAFTIPGPRL----VPPVQTPIGKVGLGVCYDLRFPELSAALQRHG-AEILTYPSAFTVATGTAHWEVLLRARAVETQCFVLAAAQVGAHHSKRASYGHALAVDPWGEVLGDCGGTQEGVTLAHINLQKLRDIRRDMPVLQHRRQTDFY 309
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Match: upb1 (ureidopropionase, beta [Source:ZFIN;Acc:ZDB-GENE-030131-1380]) HSP 1 Score: 57.7658 bits (138), Expect = 5.387e-9 Identity = 69/307 (22.48%), Postives = 128/307 (41.69%), Query Frame = 2
Query: 74 QMLKVALIQN-IVSSCKATNLSQVES-------KIKLATKSGVKLVCLPEYFNCPFG-----SEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIP--ELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYKESETSVPGNELF-TFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIAR-------------DYEAYHISYGH----SLATDPWGKVICSLDE-KEGILEFSVDLNVVDEVRSMIPIRKQERDDVYS 892
+M++V LIQN IV A L Q+ + +++A GV +VC E + PF E + +E+ +G T + +++K+ + +V + E+ ++N++++ + NG ++G K H+ + ES + GN F K + + IC+ P +Y+ N ++ P+A W + R A+ N C+ C+I R D + H +GH S P G L ++G+L +DLN+ +V + R ++Y+
Sbjct: 70 RMVRVGLIQNKIVLPTDAPVLEQITALHKRVGEMVEVAAMCGVNIVCFQEAWTMPFAFCTREREPWTEFAESAEDGLTTRFCIQLAKKHNMVVVSPILERDEIHGGTLWNTAVVVSNNGNVLGKTRKNHI--PRVGDFNESTYYMEGNTGHRVFQTQFGK-----IAVNICYGRHHPLNWLMYSVN-GAEIIFNPSATVGLLSEPMWPIEARNAAIANHCFT--CAINRVGTEYFKNEFTSGDGKKAHHDFGHFYGSSYMAAPDGSRTPGLSRTRDGLLVAELDLNLNRQVADKWNFKMTGRYEMYA 366
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Match: rasl10a (RAS like family 10 member A [Source:Xenbase;Acc:XB-GENE-493766]) HSP 1 Score: 258.455 bits (659), Expect = 6.174e-85 Identity = 125/278 (44.96%), Postives = 184/278 (66.19%), Query Frame = 2
Query: 71 IQMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+ +++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 10 VTEFRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 280
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Match: rasl10a (RAS like family 10 member A [Source:Xenbase;Acc:XB-GENE-493766]) HSP 1 Score: 258.455 bits (659), Expect = 6.372e-85 Identity = 125/275 (45.45%), Postives = 183/275 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 15 FRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 282
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Match: rasl10a (RAS like family 10 member A [Source:Xenbase;Acc:XB-GENE-493766]) HSP 1 Score: 258.84 bits (660), Expect = 6.481e-85 Identity = 125/275 (45.45%), Postives = 183/275 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 23 FRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 290
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Match: rasl10a (RAS like family 10 member A [Source:Xenbase;Acc:XB-GENE-493766]) HSP 1 Score: 257.684 bits (657), Expect = 1.568e-84 Identity = 125/275 (45.45%), Postives = 183/275 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 18 FRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 285
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Match: rasl10a (RAS like family 10 member A [Source:Xenbase;Acc:XB-GENE-493766]) HSP 1 Score: 251.906 bits (642), Expect = 2.517e-82 Identity = 123/267 (46.07%), Postives = 177/267 (66.29%), Query Frame = 2
Query: 104 IVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 21 LVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 280
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Match: Nit2 (nitrilase family, member 2 [Source:MGI Symbol;Acc:MGI:1261838]) HSP 1 Score: 256.914 bits (655), Expect = 1.407e-84 Identity = 128/278 (46.04%), Postives = 184/278 (66.19%), Query Frame = 2
Query: 71 IQMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+ ++ALIQ VSS K+ NL++ S ++ A K G +V LPE FN P+G+ +E IP GE+ Q LS+V+KE +I+++GGSIPE A K+YN+ +F P+G L+ + K+HLFD + P ++ES+T PG+ TF+ P CK VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS DPWG+V+ +E IL +DL + E+R IPI KQ+R D+Y+V
Sbjct: 1 MSTFRLALIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEKIP-GESTQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCK-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETILYSDIDLKKLAEIRQQIPILKQKRADLYTV 271
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Match: Nit2 (nitrilase family, member 2 [Source:MGI Symbol;Acc:MGI:1261838]) HSP 1 Score: 194.126 bits (492), Expect = 4.383e-61 Identity = 93/195 (47.69%), Postives = 130/195 (66.67%), Query Frame = 2
Query: 320 GSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
GSIPE A K+YN+ +F P+G L+ + K+HLFD + P ++ES+T PG+ TF+ P CK VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS DPWG+V+ +E IL +DL + E+R IPI KQ+R D+Y+V
Sbjct: 3 GSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCK-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETILYSDIDLKKLAEIRQQIPILKQKRADLYTV 191
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Match: Nit2 (nitrilase family, member 2 [Source:MGI Symbol;Acc:MGI:1261838]) HSP 1 Score: 157.532 bits (397), Expect = 1.985e-47 Identity = 75/154 (48.70%), Postives = 105/154 (68.18%), Query Frame = 2
Query: 311 IVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDP 763
++GGSIPE A K+YN+ +F P+G L+ + K+HLFD + P ++ES+T PG+ TF+ P CK VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS DP
Sbjct: 1 LIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCK-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDP 148
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Match: Nit1 (nitrilase 1 [Source:MGI Symbol;Acc:MGI:1350916]) HSP 1 Score: 148.673 bits (374), Expect = 3.181e-42 Identity = 89/277 (32.13%), Postives = 138/277 (49.82%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFN-CPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
VA+ Q + K N ++ A + G L LPE F+ + L SE + NG+ S++++EC IW+ G E + KIYN ++ N G ++ +Y K HL D P +ES + PG L P K VG+ IC+D+RFPE++ + +L YP+AF TGPA+WE+L R A+++QCYV + + SYGHS+ DPWG V+ E G+ +DL+ + ++R +P+ + R D+Y
Sbjct: 12 VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPL-NGDLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTKPGGTL----EPPVKTPAGKVGLAICYDMRFPELS-LKLAQAGAEILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 282
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Match: Nit1 (nitrilase 1 [Source:MGI Symbol;Acc:MGI:1350916]) HSP 1 Score: 149.058 bits (375), Expect = 5.047e-42 Identity = 89/277 (32.13%), Postives = 138/277 (49.82%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFN-CPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
VA+ Q + K N ++ A + G L LPE F+ + L SE + NG+ S++++EC IW+ G E + KIYN ++ N G ++ +Y K HL D P +ES + PG L P K VG+ IC+D+RFPE++ + +L YP+AF TGPA+WE+L R A+++QCYV + + SYGHS+ DPWG V+ E G+ +DL+ + ++R +P+ + R D+Y
Sbjct: 45 VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPL-NGDLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTKPGGTLE----PPVKTPAGKVGLAICYDMRFPELS-LKLAQAGAEILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 315
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Match: sp|Q6INI7|NIT2B_XENLA (Omega-amidase NIT2-B OS=Xenopus laevis OX=8355 GN=nit2b PE=2 SV=1) HSP 1 Score: 261.151 bits (666), Expect = 2.005e-85 Identity = 126/275 (45.82%), Postives = 184/275 (66.91%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ ++LS+V+KEC I+++GGSIPE + K YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F+ P CK VG+GIC+D+RF E+A IY NK C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q RD++Y+V
Sbjct: 4 FRLSLVQFLVSPVKSDNLNKACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTELLSQVAKECGIYLIGGSIPEEDSGKFYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFDTPYCK-----VGVGICYDMRFAELAQIYA-NKGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTVVSPWGEVIAKAGFEETVISADIDLQYLAEIREQIPIRRQRRDNLYTV 271
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Match: sp|Q4VBV9|NIT2_DANRE (Omega-amidase NIT2 OS=Danio rerio OX=7955 GN=nit2 PE=2 SV=1) HSP 1 Score: 260.381 bits (664), Expect = 3.850e-85 Identity = 129/278 (46.40%), Postives = 183/278 (65.83%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q VS KA NL + ++ +K A G K+V LPE FN P+G+ + +E IP GE+ Q+LS+ +K+C I++VGGSIPE K+YN+ +F P+G L+ T+ K+HLFD + P ++ESET PG L F P CK VG+GIC+D+RF E+A IY + K C LL+YP AFNM TGPA+WELL RG A+DNQ YV S ARD A ++++GHS +PWG+VI +E ++ +DL + +VR IPI KQ R+D+YSV ++
Sbjct: 4 FRLAVVQLHVSKIKADNLGRAQTLVKEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKIP-GESTQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGTLLVTHRKIHLFDIDVPGKIRFQESETLSPGKSLSMFETPYCK-----VGVGICYDIRFAELAQIYAK-KGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEESVVYADIDLQYLADVRQQIPITKQRRNDLYSVNSV 274
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Match: sp|Q28IE5|NIT2_XENTR (Omega-amidase NIT2 OS=Xenopus tropicalis OX=8364 GN=nit2 PE=2 SV=1) HSP 1 Score: 258.455 bits (659), Expect = 2.292e-84 Identity = 125/275 (45.45%), Postives = 183/275 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+++L+Q +VS K+ NL++ IK A + G ++V LPE FN P+G++ + +E IP GE+ + LS+V+KEC I+++GGSIPE + K+YN+ +F P+G L+ + K+HLFD + P ++ESET PG+ F P CK VG+GIC+D+RF E+A +Y++ K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD +A ++++GHS PWG+VI +E ++ +DL + E+R IPIR+Q R D+YSV
Sbjct: 4 FRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEKIP-GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYCK-----VGVGICYDIRFAELAQLYSK-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 271
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Match: sp|Q9NQR4|NIT2_HUMAN (Omega-amidase NIT2 OS=Homo sapiens OX=9606 GN=NIT2 PE=1 SV=1) HSP 1 Score: 258.07 bits (658), Expect = 3.992e-84 Identity = 124/275 (45.09%), Postives = 186/275 (67.64%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
++ALIQ +SS K+ N+++ S I+ A G K+V LPE FN P+G++ + +E IP GE+ Q LS+V+KEC+I+++GGSIPE A K+YN+ +F P+G L+ Y K+HLFD + P ++ES+T PG+ TF+ P C+ VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS +PWG+V+ +E I+ +DL + E+R IP+ +Q+R D+Y+V
Sbjct: 4 FRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIP-GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCR-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAV 271
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Match: sp|Q9JHW2|NIT2_MOUSE (Omega-amidase NIT2 OS=Mus musculus OX=10090 GN=Nit2 PE=1 SV=1) HSP 1 Score: 256.914 bits (655), Expect = 9.844e-84 Identity = 128/278 (46.04%), Postives = 184/278 (66.19%), Query Frame = 2
Query: 71 IQMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+ ++ALIQ VSS K+ NL++ S ++ A K G +V LPE FN P+G+ +E IP GE+ Q LS+V+KE +I+++GGSIPE A K+YN+ +F P+G L+ + K+HLFD + P ++ES+T PG+ TF+ P CK VG+GIC+D+RF E+A IY + + C LL+YP AFN+ TGPA+WELL R A+DNQ YV S ARD +A ++++GHS DPWG+V+ +E IL +DL + E+R IPI KQ+R D+Y+V
Sbjct: 1 MSTFRLALIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEKIP-GESTQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCK-----VGLGICYDMRFAELAQIYAQ-RGCQLLVYPGAFNLTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETILYSDIDLKKLAEIRQQIPILKQKRADLYTV 271
BLAST of Omega-amidase NIT2 vs. TrEMBL
Match: A0A1J1HY92 (CLUMA_CG004793, isoform A OS=Clunio marinus OX=568069 GN=putative Omega-amidase NIT2 PE=4 SV=1) HSP 1 Score: 273.478 bits (698), Expect = 1.023e-87 Identity = 129/277 (46.57%), Postives = 188/277 (67.87%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
+ L++A IQ V + K N+++ KI+ A G +LV LPE FN P+G++ + +E +P GET + LS++SKE I+++ GSIPE + NK+YN+S I++PNG LIGTY KMHLFD + P +KES++ GN TF++ K+ +G+GIC+D+RF E A IY R K CNLL+YP AFNM TGP +WELL R A DNQCY L S ARD A ++++GH++ DPW KV+ ++D +EG++ +DL++VD+VR+ IP+ Q R ++Y
Sbjct: 6 RTLRIACIQLKVGTNKTENVARALEKIREAKMKGAELVSLPECFNSPYGTKFFQQYAEAVPEGETSKFLSEISKELGIYLIAGSIPESKENKLYNTSTIWDPNGNLIGTYRKMHLFDIDIPGEITFKESDSLTAGNSFTTFDV-----KDFKIGVGICYDIRFEEFAKIY-RKKGCNLLVYPGAFNMKTGPLHWELLARARANDNQCYTALISPARDVNADYVAWGHTMVVDPWAKVVETVDSEEGLIVTDLDLSLVDKVRANIPVYNQRRSEIYDT 276
BLAST of Omega-amidase NIT2 vs. TrEMBL
Match: A0A3B5LCD1 (Nitrilase family, member 2 OS=Xiphophorus couchianus OX=32473 PE=4 SV=1) HSP 1 Score: 271.937 bits (694), Expect = 4.121e-87 Identity = 136/278 (48.92%), Postives = 185/278 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q VSS KA NLS+V +K A + G K+V LPE FN P+G+ K +E IP GE+ QMLS+V+KE + +VGGSIPE K+YN+ +F P G+LI + K+HLFD + P ++ESET PGN L TF+ P C+ VG+GIC+D+RF E+A +Y R K C LL+YP AFNM TGPA+WELL RG ALDNQ YV S ARD A ++++GHS +PWG+VI +E I+ +DL + ++R IPI Q RDD+Y+VT +
Sbjct: 4 FRLAVVQLQVSSVKADNLSKVRRLVKEAAEQGSKVVLLPECFNSPYGTSFFSKYAEKIP-GESTQMLSEVAKENKLHLVGGSIPEEDGGKLYNTCAVFGPEGELILKHRKIHLFDIDIPGKIRFQESETLSPGNSLSTFDTPFCR-----VGVGICYDMRFAELAQLYNR-KGCQLLVYPGAFNMTTGPAHWELLQRGRALDNQMYVATASPARDEAASYVAWGHSTVVNPWGEVIAKAGPEESIIYADIDLQYLADIRQQIPISSQRRDDLYAVTAV 274
BLAST of Omega-amidase NIT2 vs. TrEMBL
Match: A0A3B5L7B0 (Nitrilase family, member 2 OS=Xiphophorus couchianus OX=32473 PE=4 SV=1) HSP 1 Score: 271.937 bits (694), Expect = 4.799e-87 Identity = 136/278 (48.92%), Postives = 185/278 (66.55%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q VSS KA NLS+V +K A + G K+V LPE FN P+G+ K +E IP GE+ QMLS+V+KE + +VGGSIPE K+YN+ +F P G+LI + K+HLFD + P ++ESET PGN L TF+ P C+ VG+GIC+D+RF E+A +Y R K C LL+YP AFNM TGPA+WELL RG ALDNQ YV S ARD A ++++GHS +PWG+VI +E I+ +DL + ++R IPI Q RDD+Y+VT +
Sbjct: 11 FRLAVVQLQVSSVKADNLSKVRRLVKEAAEQGSKVVLLPECFNSPYGTSFFSKYAEKIP-GESTQMLSEVAKENKLHLVGGSIPEEDGGKLYNTCAVFGPEGELILKHRKIHLFDIDIPGKIRFQESETLSPGNSLSTFDTPFCR-----VGVGICYDMRFAELAQLYNR-KGCQLLVYPGAFNMTTGPAHWELLQRGRALDNQMYVATASPARDEAASYVAWGHSTVVNPWGEVIAKAGPEESIIYADIDLQYLADIRQQIPISSQRRDDLYAVTAV 281
BLAST of Omega-amidase NIT2 vs. TrEMBL
Match: A0A369SCF5 (Omega-amidase NIT2 OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_004381 PE=4 SV=1) HSP 1 Score: 271.552 bits (693), Expect = 6.032e-87 Identity = 130/275 (47.27%), Postives = 184/275 (66.91%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
++ +V LIQ +VS+ K NL ++ ++ A + G KL+ LPE FN P+G++ SE +P G T Q+LS ++KE +++VGGSIPE+ K++N+ +F+P G +I Y KMHLFD + P ++ES+ PGNE TF+ P CK VG+GIC+D+RFPE+A Y + + C L++YP AFN TGP +WELL R ALDNQ YV CS ARD A +I++GHS DPWG+V+ + DEKE I+ +DLN +DEVRS IP+ Q+R D+Y
Sbjct: 3 RVFRVGLIQLMVSTNKNDNLHRIHKFVREAAQQGAKLISLPECFNSPYGTKFFKAYSETVP-GTTTQVLSDIAKENKVYLVGGSIPEVADGKLFNTCTVFDPEGSMIAKYRKMHLFDIDVPGKITFRESDVLSPGNEFATFDTPYCK-----VGLGICYDVRFPELAQAYCK-RGCQLVIYPGAFNTTTGPVHWELLQRSRALDNQIYVATCSPARDKTADYITWGHSTVVDPWGRVMSTCDEKEQIVYADIDLNYLDEVRSQIPVTAQKRYDIY 270
BLAST of Omega-amidase NIT2 vs. TrEMBL
Match: B3RTR0 (CN hydrolase domain-containing protein OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_56014 PE=4 SV=1) HSP 1 Score: 271.552 bits (693), Expect = 6.032e-87 Identity = 130/275 (47.27%), Postives = 184/275 (66.91%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
++ +V LIQ +VS+ K NL ++ ++ A + G KL+ LPE FN P+G++ SE +P G T Q+LS ++KE +++VGGSIPE+ K++N+ +F+P G +I Y KMHLFD + P ++ES+ PGNE TF+ P CK VG+GIC+D+RFPE+A Y + + C L++YP AFN TGP +WELL R ALDNQ YV CS ARD A +I++GHS DPWG+V+ + DEKE I+ +DLN +DEVRS IP+ Q+R D+Y
Sbjct: 3 RVFRVGLIQLMVSTNKNDNLHRIHKFVREAAQQGAKLISLPECFNSPYGTKFFKAYSETVP-GTTTQVLSDIAKENKVYLVGGSIPEVADGKLFNTCTVFDPEGSMIAKYRKMHLFDIDVPGKITFRESDVLSPGNEFATFDTPYCK-----VGLGICYDVRFPELAQAYCK-RGCQLVIYPGAFNTTTGPVHWELLQRSRALDNQIYVATCSPARDKTADYITWGHSTVVDPWGRVMSTCDEKEQIVYADIDLNYLDEVRSQIPVTAQKRYDIY 270
BLAST of Omega-amidase NIT2 vs. Ensembl Cavefish
Match: nit2 (nitrilase family member 2 [Source:NCBI gene;Acc:103034042]) HSP 1 Score: 251.906 bits (642), Expect = 1.141e-82 Identity = 124/278 (44.60%), Postives = 178/278 (64.03%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q V+ K+ NL + S +K A G K+V LPE FN P+G+ + +E IP GE+ Q LS+ +KE +++VGGSIPE K++N+ +F P+G+++ + K+HLFD + P ++ESET PG L F P CK VG+GIC+D+RF E+A IY R K C LL+YP AFNM TGPA+WELL R ALDNQ YV S ARD A ++++GHS +PWG+VI +E ++ +DL + +VR IPI Q R+D+YSV+ +
Sbjct: 11 FRLAVVQLAVTKVKSDNLQRACSLVKEAAGQGAKVVVLPECFNSPYGTGFFAEYAEKIP-GESTQALSEAAKESGVFLVGGSIPEEDGEKLFNTCSVFGPDGKILVKHRKIHLFDIDVPGKIRFQESETLSPGCSLSMFETPFCK-----VGVGICYDMRFAELAQIYAR-KGCQLLVYPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDETASYVAWGHSTVVNPWGEVITKAGSEESVVYADIDLQYLSDVRQQIPITTQRRNDIYSVSAV 281
BLAST of Omega-amidase NIT2 vs. Ensembl Cavefish
Match: nit1 (nitrilase 1 [Source:NCBI gene;Acc:103037987]) HSP 1 Score: 152.525 bits (384), Expect = 1.323e-43 Identity = 94/279 (33.69%), Postives = 151/279 (54.12%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-----KIYNSSMIFNPNGQLIGTYSKMHLFD---SNRPI-YKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKE-GILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
A+ Q + K N + E +K A + +V LPE F+ S E+ L+ SE++ G+T +K++KE +W+ G E + +IYNS +I N G ++ Y K HLFD +NR + KES ++PG L +P + VG+GIC+DLRFPE++ + + + +L YP+AF +ATG A+WE+L R A++ QC+V + + A SYGH+LA DPWG V+ E G+ ++L + + R +P+++ +R Y
Sbjct: 41 AAVCQMTSTPDKEANFTTCERLVKQAKEGRASMVFLPEGFDYIGSSREETLQLSESL-QGDTISRYTKLAKELNVWLSLGGFHERGHDWENDRRIYNSHVIINEQGGIVSVYRKGHLFDVELANRGVSLKESAFTIPGPSL----VPPVETPVGKVGLGICYDLRFPELS-LALQRQGAEILTYPSAFTVATGAAHWEVLLRARAIETQCFVLAAAQVGAHHAKRSSYGHALAVDPWGVVMGDCGGTEGGVALVEINLQRLQDTRRDMPVQQHKRQSEY 313
BLAST of Omega-amidase NIT2 vs. Ensembl Cavefish
Match: upb1 (beta-ureidopropionase 1 [Source:NCBI gene;Acc:103031480]) HSP 1 Score: 58.151 bits (139), Expect = 3.705e-9 Identity = 70/307 (22.80%), Postives = 125/307 (40.72%), Query Frame = 2
Query: 74 QMLKVALIQN-IVSSCKATNLSQVES-------KIKLATKSGVKLVCLPEYFNCPFG-----SEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIP--ELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYKESETSVPGNELF-TFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIAR-------------DYEAYHISYGH----SLATDPWGKVICSLDE-KEGILEFSVDLNVVDEVRSMIPIRKQERDDVYS 892
+ ++V LIQN IV A L Q+ + + +A GV +VC E + PF + +E+ G T + +++K+ + +V + EL ++N++++ + G ++G K H+ + ES + GN FN K +G+ IC+ P +Y+ N ++ P+A W + R A+ N C+ C+I R D + H +GH S P G L ++G+L +DLN+ +V + R D+Y+
Sbjct: 70 RTVRVGLIQNKIVLPTDAPVLEQITALHKRVGEMVDVAAACGVNIVCFQEAWTMPFAFCTRERAPWTEFAESAEGGFTTRFCKELAKKYNMVVVSPILERDELHGGTLWNTAVVVSNTGNVLGKSRKNHI--PRVGDFNESSYYMEGNTRHRVFNTQYGK-----IGVNICYGRHHPLNWLMYSMN-GAEIIFNPSATVGELSEPMWPIEARNAAIANHCFT--CAINRVGTEYFKNEFTSGDGKKAHHEFGHFYGSSYVAAPDGTRSPGLSRIRDGLLVAELDLNLNRQVADKWNFKMTGRYDMYA 366
BLAST of Omega-amidase NIT2 vs. Ensembl Yeast
Match: NIT3 (Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member [Source:SGD;Acc:S000004343]) HSP 1 Score: 210.305 bits (534), Expect = 3.443e-67 Identity = 121/284 (42.61%), Postives = 171/284 (60.21%), Query Frame = 2
Query: 74 QMLKVALIQNIVSSC-KATNLSQVESKIKLATKS--GVKLVCLPEYFNCPFGSEDLLKQSENIPNGE---TYQMLSKVSKECAIWIVGGSIPEL--RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYS 892
Q +KVAL+Q SS K NL + + I+ A K KLV LPE FN P+ ++ K SE I E + Q LS ++ + I +VGG+IPEL + +KIYN+S+IFN +G+LI + K+HLFD + P + ESET PG + T + K G+GIC+D+RFPE+A + R K ++YP+AFN TGP +W LL R A+DNQ YV LCS AR+ ++ + +YGHS+ DP GK++ E E I+ +D V++ R +P+ KQ R DVYS
Sbjct: 9 QKIKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQFRKYSEVINPKEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISFHESETLSPGEKSTTIDTKYGK-----FGVGICYDMRFPELAMLSAR-KGAFAMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKIVAEAGEGEEIIYAELDPEVIESFRQAVPLTKQRRFDVYS 286
BLAST of Omega-amidase NIT2 vs. Ensembl Yeast
Match: NIT2 (Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member [Source:SGD;Acc:S000003662]) HSP 1 Score: 117.472 bits (293), Expect = 2.993e-31 Identity = 68/207 (32.85%), Postives = 110/207 (53.14%), Query Frame = 2
Query: 344 NKIYNSSMIFNPNGQLIGTYSKMHLFD---SNRPIYKESETSVPGNEL-FTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCY------VGLCSI------------ARDYEAYHISYGHSLATDPWGKVICSLDEKE---GILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
+++ N + + G+++ Y K+HLFD N PI KES++ PG + PL K +G IC+D+RFPE + + R+ +L +P+AF + TG A+WELLGR A+D QCY VG+ + A + + S+GHS+ DPWGK+I D ++ +D ++ E+R+ +P+ Q RDD++
Sbjct: 106 DRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIPDIIESPLGK-----LGSAICYDIRFPEFS-LKLRSMGAEILCFPSAFTIKTGEAHWELLGRARAVDTQCYVLMPGQVGMHDLSDPEWEKQSHMSALEKSSRRESWGHSMVIDPWGKIIAHADPSTVGPQLILADLDRELLQEIRNKMPLWNQRRDDLF 306
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Match: EDO30709 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T002]) HSP 1 Score: 263.077 bits (671), Expect = 1.505e-87 Identity = 132/279 (47.31%), Postives = 185/279 (66.31%), Query Frame = 2
Query: 71 IQMLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-KIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSV 895
I + ++ L+Q V++ K NL + KIK A +G K+V LPE FN P+G++ +E IP GE+ ML++V+KE +IVGGSIPE +N K+YN+S+ ++P+G L+G + K+HLFD + P ++ESE PG L + CK +GIGIC+D+RFPE+A +Y + K C+LLLYP AFNM TGPA+WELL R ALDNQ YV S ARD A +I++GHS +PWGK++ D E IL +DL ++EVRS IP++ Q+RDDVY +
Sbjct: 5 ILVFRIGLVQLAVTANKLQNLQRAREKIKEAVAAGAKIVALPECFNSPYGTQYFKDYAEEIP-GESSNMLAEVAKETGAYIVGGSIPERASNGKLYNTSLSYDPSGNLMGKHRKIHLFDIDVPGKIRFQESEVLSPGENLTILDTEYCK-----IGIGICYDMRFPELAQLYAK-KGCHLLLYPGAFNMTTGPAHWELLTRARALDNQLYVATISPARDDNATYIAWGHSTVVNPWGKIVSKADHTEQILYAEIDLKYLNEVRSQIPVQFQKRDDVYEL 276
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Match: EDO27394 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9D3]) HSP 1 Score: 150.599 bits (379), Expect = 3.632e-45 Identity = 78/160 (48.75%), Postives = 109/160 (68.12%), Query Frame = 2
Query: 197 EYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRAN-KIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRG 664
E FN P+G++ +E IP GE+ ML++V+KE +IVGGSIPE +N K+YN+S+ ++P+G L+G + K+HLFD + P ++ESE PG L + CK +GIGIC+D+RFPE+A +Y + K C+LLLYP AFNM TGPA+WELL R
Sbjct: 1 ECFNSPYGTQYFKDYAEEIP-GESSNMLAEVAKETGAYIVGGSIPERASNRKLYNTSLSYDPSGNLMGKHRKIHLFDIDVPGKIRFQESEVLSPGENLTILDTEYCK-----IGIGICYDMRFPELAQLYAK-KGCHLLLYPGAFNMTTGPAHWELLTRA 153
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Match: EDO47206 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMU0]) HSP 1 Score: 137.502 bits (345), Expect = 1.256e-38 Identity = 83/276 (30.07%), Postives = 142/276 (51.45%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPF-GSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIP---ELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSN---RPIYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYV-GLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
+A+ Q ++ N Q + I+ ++ G ++V LPE F+ E +L+ +E++ +G + K+++E +W+ G ++ N ++ + G++ +Y+K HLFD N P KES G+ + +P +G+GIC+DLRFPE + I R + ++L +P+AF TG A+WE+L R A++ QCYV + YE +YGHS+ DPWG V+ + G+ +D + +VRS +PI R D+Y
Sbjct: 9 IAICQMTCTADLEANFRQCQELIRKGSRKGAEVVFLPEGFDFLMKDKEKILELAEHL-DGPRISKMCKLAEENGVWLSLGGFHCKHPSETRRVLNCHVVIDNKGRIAASYNKTHLFDVNIEGGPCLKESAFIAHGDRI----VPPVSTPVGKLGLGICYDLRFPEFSMILAR-QGADILSFPSAFTFHTGSAHWEVLLRSRAIETQCYVVAAAQCGKLYEGRE-AYGHSMVVDPWGTVVAQCQDGIGLCMAEIDHQYIQKVRSGMPILSHRRVDLY 277
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Match: EDO25888 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TDF7]) HSP 1 Score: 85.1149 bits (209), Expect = 1.398e-20 Identity = 43/124 (34.68%), Postives = 70/124 (56.45%), Query Frame = 2
Query: 521 VGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYV---GLCSI--ARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQER 877
VG+ IC+DLRFP++ + R + + PAAF TG A+W+ L R A++ QCYV C A D ++GHS DPWG+V+C L E+ G+ +++ ++R+ +P+ + +R
Sbjct: 7 VGLAICYDLRFPQLF-MQMRAQGAEIFTLPAAFTHKTGAAHWQSLLRARAIEQQCYVIAANQCGWHDAPDQPQRRQTWGHSQIIDPWGEVLCELAEQPGLAVANLNPQQQQQIRNNMPLFQHQR 129
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Match: EDO26935 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TAN6]) HSP 1 Score: 69.3218 bits (168), Expect = 4.495e-14 Identity = 47/192 (24.48%), Postives = 90/192 (46.88%), Query Frame = 2
Query: 278 LSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSM 853
+++K+ IW++ G++ + + +KIYN++ + NP G+++G Y+KM P Y G+E +++P + G+ IC+D+ FPE + K ++L+P ++ + A NQCY+ + D G SL P G+V+ + + ++ +DL+ V R M
Sbjct: 28 FREMAKKHNIWLIPGTMFQKKEDKIYNTATVINPEGEVVGRYNKMF------PFYPYEVGVTGGDEFLIWDVPNVGK----FGLTICYDMWFPETSRTLAV-KGVEVILHPTLTGTIDRDIELAIV-QATAATNQCYIFDINGLGDG-----GTGRSLVCGPDGRVLYQANTGDEMIPMEIDLDRVRRSREM 202
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Match: nit2 (nitrilase family member 2 [Source:NCBI gene;Acc:101170666]) HSP 1 Score: 266.159 bits (679), Expect = 3.987e-88 Identity = 130/278 (46.76%), Postives = 183/278 (65.83%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q V+S KA NLS+ + A G K+V LPE FN P+G+ +E IP GE+ QMLS+ +KE +++VGGSIPE K+YN+ +F P G++I + K+HLFD N P ++ESET PGN L TF+ P CK VG+GIC+D+RF E+A +Y+R + C LL+YP AFNM TGPA+WELL RG A+DNQ YV S ARD A ++++GHS +PWG+VI +E ++ +DL + ++R IPI Q RDD+YSVT++
Sbjct: 11 FRLAVVQLQVTSVKADNLSRARRLVLQAAGQGSKVVLLPECFNSPYGTSFFSDYAEKIP-GESTQMLSEAAKENQVYLVGGSIPEEDGGKLYNTCAVFGPGGEMILKHRKIHLFDINVPGKICFQESETLSPGNSLSTFDTPFCK-----VGVGICYDMRFAELAQVYSR-EGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDEAASYVAWGHSTVVNPWGEVISKAGAEETVIYADIDLQYLSDIRRQIPITAQRRDDLYSVTSV 281
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Match: nit2 (nitrilase family member 2 [Source:NCBI gene;Acc:101170666]) HSP 1 Score: 244.202 bits (622), Expect = 1.133e-79 Identity = 127/278 (45.68%), Postives = 177/278 (63.67%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTTL 904
++A++Q V+S KA NLS+ + A G K+V LPE FN P+G+ +E IP GE+ QMLS+ +KE +++VGGSIPE K+YN+ +F P G++I + K+HLFD N P ++ESET PGN L TF+ P CK VG+GIC+D+RF E+A +Y+R + C LL+YP AFNM TGPA+WELL RG A+DNQ YV S ARD A ++++GHS +P G +L + DL + ++R IPI Q RDD+YSVT++
Sbjct: 4 FRLAVVQLQVTSVKADNLSRARRLVLQAAGQGSKVVLLPECFNSPYGTSFFSDYAEKIP-GESTQMLSEAAKENQVYLVGGSIPEEDGGKLYNTCAVFGPGGEMILKHRKIHLFDINVPGKICFQESETLSPGNSLSTFDTPFCK-----VGVGICYDMRFAELAQVYSR-EGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDEAASYVAWGHSTVVNPSGSA--ALTPPLCV----ADLQYLSDIRRQIPITAQRRDDLYSVTSV 268
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Match: nit1 (nitrilase 1 [Source:NCBI gene;Acc:101174868]) HSP 1 Score: 148.673 bits (374), Expect = 4.236e-42 Identity = 93/280 (33.21%), Postives = 145/280 (51.79%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP----IYKESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSL-DEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
A+ Q + K N S + ++ A + G + LPE F+ S E+ L SE++ G+T KV+++ +W+ G E R +IYNS +I N G ++ Y K HLFD P ES+ ++PG L + P+ K VG+GIC+DLRF E++ R+ +L YP+AF +ATG A+WE+L R A++ QC+V + + SYGHSLA DPWG+V+ E ++ VDL+ V R +P+++ R+ +
Sbjct: 37 AAICQLTATPNKEANFSICKKLVEEAKEKGASMAFLPEGFDYIGSSREETLSLSESLA-GDTIAGYIKVARQLDVWLSLGGFHEQGPEWEREKRIYNSHIIINNKGDIVSVYRKSHLFDVKLPEKGISLMESDFTLPGPSLVSPVQTPIGK-----VGLGICYDLRFSELSLALQRSG-AEILTYPSAFTVATGAAHWEVLLRARAIETQCFVLAAAQVGQHHEKRASYGHSLAVDPWGEVLADCGGESPAVVLVEVDLDKVRRTRKNMPVQQHRRETAF 309
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Match: nit1 (nitrilase 1 [Source:NCBI gene;Acc:101174868]) HSP 1 Score: 148.288 bits (373), Expect = 4.373e-42 Identity = 93/280 (33.21%), Postives = 145/280 (51.79%), Query Frame = 2
Query: 86 VALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPEL-----RANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP----IYKESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSL-DEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
A+ Q + K N S + ++ A + G + LPE F+ S E+ L SE++ G+T KV+++ +W+ G E R +IYNS +I N G ++ Y K HLFD P ES+ ++PG L + P+ K VG+GIC+DLRF E++ R+ +L YP+AF +ATG A+WE+L R A++ QC+V + + SYGHSLA DPWG+V+ E ++ VDL+ V R +P+++ R+ +
Sbjct: 23 AAICQLTATPNKEANFSICKKLVEEAKEKGASMAFLPEGFDYIGSSREETLSLSESL-AGDTIAGYIKVARQLDVWLSLGGFHEQGPEWEREKRIYNSHIIINNKGDIVSVYRKSHLFDVKLPEKGISLMESDFTLPGPSLVSPVQTPIGK-----VGLGICYDLRFSELSLALQRSG-AEILTYPSAFTVATGAAHWEVLLRARAIETQCFVLAAAQVGQHHEKRASYGHSLAVDPWGEVLADCGGESPAVVLVEVDLDKVRRTRKNMPVQQHRRETAF 295
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Match: upb1 (beta-ureidopropionase 1 [Source:NCBI gene;Acc:101160078]) HSP 1 Score: 52.373 bits (124), Expect = 2.699e-7 Identity = 66/290 (22.76%), Postives = 121/290 (41.72%), Query Frame = 2
Query: 83 KVALIQN-IVSSCKATNLSQVESK-------IKLATKSGVKLVCLPEYFNCPFG-----SEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIP-ELRANKIYNSSMIFNPNGQLIGTYSKMHL---FDSNRPI-YKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIAR-------------DYEAYHISYGH----SLATDPWGKVICSLDE-KEGILEFSVDLNVVDEV 844
+V L+Q+ IV A L Q+ + +++A GV ++C E + PF E + +E+ G T + +++K+ + I+ + E +K++NS+++ + +G ++G K H+ D N Y E +T G+ +F + + IC+ P +Y+ N ++ P+A A W + R A+ N C+ C+I R D H +GH S P G L ++G+L +DLN+ +V
Sbjct: 73 RVGLVQHKIVLPTDAPVLEQINAIHSRVGEIVEVAAMCGVNIICFQETWTMPFAFCTREKEPWTEFAESAEEGNTTRFCQELAKKYNMVIISPILEREELHSKLWNSAVVISNSGNVLGKSRKNHIPRVGDFNESTYYMEGDT---GHTVFQTQFG-------RIAVNICYGRHHPLNWFMYSMN-GAEIIFNPSATVGALSEPMWPIEARNAAIANHCFT--CAINRVGTEHFKNEFTSGDGRKAHHDFGHFYGSSYVAAPDGSRTPGLSRTQDGLLVVEMDLNLNRQV 349
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Match: SMESG000071423.1 (SMESG000071423.1) HSP 1 Score: 355.14 bits (910), Expect = 2.390e-123 Identity = 168/278 (60.43%), Postives = 216/278 (77.70%), Query Frame = 2
Query: 77 MLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTT 901
MLKVALIQ +V++ K+ NL + SKIKLA G KL+CLPE FNCP+G + + ENIPN +T + LS ++KE +IW+VGGSIPE R KIYN+ ++FNPNG+L+GTYSK+HLFD P +++ES T GN+LF+F+IP+ +K +TVG+GIC+D+RF E+A IYTR CNLLLYP AFNM TGP +WELL R A+DNQCYVGLCS ARD A ++SYGHSLA DPWGKV+ SL+EK+ ILEF+V+L+ VD VRSMIPI +Q R+D+Y+V T
Sbjct: 1 MLKVALIQMMVTANKSDNLLKACSKIKLAADKGAKLICLPECFNCPYGIKYFPEYCENIPNEKTSKSLSDIAKEHSIWLVGGSIPESREGKIYNTCLVFNPNGELVGTYSKIHLFDIKIPGKMVFQESTTLTAGNKLFSFDIPVDNDKKVTVGLGICYDVRFAELAHIYTRKHNCNLLLYPGAFNMTTGPLHWELLARFRAMDNQCYVGLCSTARDENAEYVSYGHSLAADPWGKVLGSLNEKDDILEFTVNLDEVDNVRSMIPIGRQRREDIYTVAT 278
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Match: SMESG000071423.1 (SMESG000071423.1) HSP 1 Score: 340.887 bits (873), Expect = 6.750e-118 Identity = 160/269 (59.48%), Postives = 208/269 (77.32%), Query Frame = 2
Query: 104 IVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTT 901
+V++ K+ NL + SKIKLA G KL+CLPE FNCP+G + + ENIPN +T + LS ++KE +IW+VGGSIPE R KIYN+ ++FNPNG+L+GTYSK+HLFD P +++ES T GN+LF+F+IP+ +K +TVG+GIC+D+RF E+A IYTR CNLLLYP AFNM TGP +WELL R A+DNQCYVGLCS ARD A ++SYGHSLA DPWGKV+ SL+EK+ ILEF+V+L+ VD VRSMIPI +Q R+D+Y+V T
Sbjct: 1 MVTANKSDNLLKACSKIKLAADKGAKLICLPECFNCPYGIKYFPEYCENIPNEKTSKSLSDIAKEHSIWLVGGSIPESREGKIYNTCLVFNPNGELVGTYSKIHLFDIKIPGKMVFQESTTLTAGNKLFSFDIPVDNDKKVTVGLGICYDVRFAELAHIYTRKHNCNLLLYPGAFNMTTGPLHWELLARFRAMDNQCYVGLCSTARDENAEYVSYGHSLAADPWGKVLGSLNEKDDILEFTVNLDEVDNVRSMIPIGRQRREDIYTVAT 269
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Match: SMESG000071423.1 (SMESG000071423.1) HSP 1 Score: 340.502 bits (872), Expect = 8.505e-118 Identity = 160/269 (59.48%), Postives = 208/269 (77.32%), Query Frame = 2
Query: 104 IVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFTFNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEKEGILEFSVDLNVVDEVRSMIPIRKQERDDVYSVTT 901
+V++ K+ NL + SKIKLA G KL+CLPE FNCP+G + + ENIPN +T + LS ++KE +IW+VGGSIPE R KIYN+ ++FNPNG+L+GTYSK+HLFD P +++ES T GN+LF+F+IP+ +K +TVG+GIC+D+RF E+A IYTR CNLLLYP AFNM TGP +WELL R A+DNQCYVGLCS ARD A ++SYGHSLA DPWGKV+ SL+EK+ ILEF+V+L+ VD VRSMIPI +Q R+D+Y+V T
Sbjct: 2 MVTANKSDNLLKACSKIKLAADKGAKLICLPECFNCPYGIKYFPEYCENIPNEKTSKSLSDIAKEHSIWLVGGSIPESREGKIYNTCLVFNPNGELVGTYSKIHLFDIKIPGKMVFQESTTLTAGNKLFSFDIPVDNDKKVTVGLGICYDVRFAELAHIYTRKHNCNLLLYPGAFNMTTGPLHWELLARFRAMDNQCYVGLCSTARDENAEYVSYGHSLAADPWGKVLGSLNEKDDILEFTVNLDEVDNVRSMIPIGRQRREDIYTVAT 270
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Match: SMESG000071424.1 (SMESG000071424.1) HSP 1 Score: 247.669 bits (631), Expect = 3.407e-83 Identity = 122/132 (92.42%), Postives = 124/132 (93.94%), Query Frame = 2
Query: 77 MLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPIYKESETSVPGN 472
MLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP + + GN
Sbjct: 1 MLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEDLLKQSENIPNGETYQMLSKVSKECAIWIVGGSIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRPRMPDFRKIISGN 132
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Match: SMESG000072713.1 (SMESG000072713.1) HSP 1 Score: 140.198 bits (352), Expect = 4.840e-38 Identity = 93/280 (33.21%), Postives = 152/280 (54.29%), Query Frame = 2
Query: 80 LKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGS-EDLLKQSENIPNGETYQMLSKVSKECAIWI-VGG---SIPELRANKIYNSSMIFNPNGQLIGTYSKMHLFDSNRP---IYKESETSVPGNELFT-FNIPLCKEKNITVGIGICHDLRFPEVAGIYTRNKKCNLLLYPAAFNMATGPAYWELLGRGIALDNQCYVGLCSIARDYEAYHISYGHSLATDPWGKVICSLDEK-EGILEFSVDLNVVDEVRSMIPIRKQERDDVY 889
+K+ +IQ +S K N + I+ A+K+G K+ LPE F+ S E L SE+I +G+ K++ + I++ +GG +P+ + KIYN+ ++ + G + Y K+HLFD + P ++ES G+ + PL + +G+ IC+DLRFPE+ G R N+L YP+AF + TG A+W +L R A++NQC+V + + SYGHSL DPWG +I E G++ +DL+V+ +VR +P+ R D++
Sbjct: 5 VKIGVIQLRSTSNKLENFKICKKFIEEASKNGAKMAFLPENFDFMSESKEQTLSLSESI-DGDLISNYRKIAVDKNIYLSLGGFHRKVPQ-ESGKIYNTHVVIDNEGHIKEIYDKLHLFDCDIPNLESFRESSVVKAGDSVRPPVETPLGR-----MGLSICYDLRFPEL-GQTLRQSLANVLTYPSAFTVKTGEAHWHILLRCRAIENQCFVIAAAQEGQHNPKRASYGHSLVVDPWGTIIAEHTETGPGLMMCDIDLSVIGKVRLNLPVFAHRRLDLF 276 The following BLAST results are available for this feature:
BLAST of Omega-amidase NIT2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Omega-amidase NIT2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of Omega-amidase NIT2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Omega-amidase NIT2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Omega-amidase NIT2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of Omega-amidase NIT2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Omega-amidase NIT2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Omega-amidase NIT2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Omega-amidase NIT2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032965 ID=SMED30032965|Name=Omega-amidase NIT2|organism=Schmidtea mediterranea sexual|type=transcript|length=944bpback to top protein sequence of SMED30032965-orf-1 >SMED30032965-orf-1 ID=SMED30032965-orf-1|Name=SMED30032965-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=278bp MLKVALIQNIVSSCKATNLSQVESKIKLATKSGVKLVCLPEYFNCPFGSEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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