SMED30032949

Overview
NameSMED30032949
Smed IDSMED30032949
Length (bp)1884
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032949 (SMED30032949) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032949 (SMED30032949) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032949 (SMED30032949) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032949 (SMED30032949) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032949

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30032949SMESG000004884.1 Contig761GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30032949SMESG000022823.1 Contig761GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
parenchymal cellSMED30032949SMESG000022823.1 dd_Smed_v4_8783_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30032949SMESG000022823.1 dd_Smed_v6_8783_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032949 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002110:47855..56059 -9171
Homology
BLAST of SMED30032949 vs. Planmine SMEST
Match: SMESG000022823.1 (SMESG000022823.1)

HSP 1 Score: 1080.47 bits (2793), Expect = 0.000e+0
Identity = 535/543 (98.53%), Postives = 539/543 (99.26%), Query Frame = 3
Query:  198 MSNLQISIDEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKCKLLEIGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITDLLIGSICCFCEMRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNFDPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVKSSKDFSEWKCSKCGLLNYDEFCMACLNEKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSSMESEKCVICRNETNCNCKSNTKNSAITNENHKETLVENWENNKNNDASATNINSDQWKCDKCGMLNYDDFCSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKCKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSIKDISGMFWLCKKCSAINDYEKKVCTSFSCKQKFDFTTHKCEICRKKFRGNSCKYCETKCPTCFYNRCEKCSILPKFKTN 1826
            MSNLQISIDEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKCKLL+IGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITDLLIGSICCFCEMRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNFDPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVK+SKDFSEWKCSKCGLLNYDEFCMACLNEKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSS+ESEKCVICRNETNCNCKSNTKNSAITNENHKETLVENWE   NNDAS TNINSDQWKCDKCGMLNYDDFCSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCK+CKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSIKDISGMFWLCKKCSAINDYEKKVCTSFSCKQKFDFTTHKCEICRKKFRGNSCKYCETKCPTCFYNRCEKCSILPKFKTN
Sbjct:    1 MSNLQISIDEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKCKLLDIGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITDLLIGSICCFCEMRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNFDPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVKTSKDFSEWKCSKCGLLNYDEFCMACLNEKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSSIESEKCVICRNETNCNCKSNTKNSAITNENHKETLVENWE---NNDASTTNINSDQWKCDKCGMLNYDDFCSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKQCKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSIKDISGMFWLCKKCSAINDYEKKVCTSFSCKQKFDFTTHKCEICRKKFRGNSCKYCETKCPTCFYNRCEKCSILPKFKTN 540          
BLAST of SMED30032949 vs. Planmine SMEST
Match: SMESG000022833.1 (SMESG000022833.1)

HSP 1 Score: 60.077 bits (144), Expect = 3.840e-9
Identity = 110/475 (23.16%), Postives = 187/475 (39.37%), Query Frame = 3
Query:  168 EDILYANRSVMSNLQISIDEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKCKLLEIGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITD-LLIGSICCFCE-------------MRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNF------DPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVKSSKDF-----------SEWKCSKCGL---LNYDEFCMACLNEKVITNQKYGQEINLEKSGA---KNSVEKRNTTSTYRYENVSSMES-EKCVICRNETNCNCKSNTKNSAITNENHKETLVENWENNKNNDASATNINSDQWKCDKCGMLN-YDDF-CSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKC-KSIKIAADRRFCDTC 1469
            E++LY N  + ++  IS   +N Y  + LY   ++C   AQ L  +Y        C L     + N  +    G E ++    ++++E +  ++  H ++Y +E+   + K+    D ++IG IC  CE             M  D      NV +    D++  ++N  + L  V      K +    +   + ++      D    LN  + + ++ +     S+ +   +    N+  K  ++L  + ENI K S DF           + W+C  C       + +FC  CL +K   +Q   +  N++K      K  +EK N    +  E  S   S  +C    ++T+  C     N+   N +H    VE                 D W C KC M N +D + C  C         W CE+C   N+     C+ C   N N+  CKKC K+  I  D  FC +C
Sbjct:   55 EEVLYVN--LAASKAISQLAWNAYQNNGLY---KDCHINAQYLEKIYFI---MGNCNL-----YTNYLDKFKLGTEFLE----NVLIEMLSRYIIIHCILYYKEIDNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLN-SSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDK--QSQIDSENKNMDKISVTEDKILIEKEN----FNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENISHD---VE-----------------DTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSC 485          
BLAST of SMED30032949 vs. Planmine SMEST
Match: SMESG000022814.1 (SMESG000022814.1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.416e-8
Identity = 85/351 (24.22%), Postives = 131/351 (37.32%), Query Frame = 3
Query:  897 WKC--SKCGLLNY--DEFCMACLNEKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSSMESEKCVIC---RNETNCNCKSNTKNSAITNENHKETLVENWE--------NNKNNDASATNINSDQ---------------------WKCDKCGMLNYD---DFCSACLNER------------------RTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKCKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSI---KDISG---MFWLCKKCSAINDYEKKVCTS------------FSCKQKFDFTTHKCEICRK 1724
            WKC  S CG  N   D++C  C  ++   NQ  G+ IN+ K G K  +        +       ++S+KC IC   R E   N K       + N    E + ENW+         N  N    ++   D+                     W C KCG+ N D   D C  C   R                    +  WKC +C+ +N++ +  C +CG+  E  +LC  C S +  A    C  C      W CW   C  +N+   +YC  C   +     + I   + +SG     W+C+KC+ IN      C +            + CK   +++ +KC  C +
Sbjct:  401 WKCQQSICGKANVGSDKYCSRCGVDRA-ANQNRGEIINVLKDGRKEKMWICTKCGLFN----GDLDSDKCEICECSRKEEKEN-KYEQNRGRLGNPAGGE-IDENWDCWSRSCRKRNYGNHQYCSHCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGDLDSDKCEICEEMRYEKDAYEIITDVEMKRAAGVLNDWKCGKCECVNNYDLVSCKACGEIKE--KLCNIC-SERFQAAGEICPGC---NARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKENNYYCWANGYCPGRRGRLCYYCKCWVEYSQYKCPNCDR 738          
BLAST of SMED30032949 vs. Planmine SMEST
Match: SMESG000022780.1 (SMESG000022780.1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.081e-7
Identity = 56/212 (26.42%), Postives = 80/212 (37.74%), Query Frame = 3
Query: 1245 WKCDKCGMLNYD---DFCSACLNER------------------RTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKCKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSI---KDISG---MFWLCKKCSAIN-DYEKKVCTSFSCKQKFDFTTHKCEICRKKFRGNSCKYC-------ETKCPTC 1775
            W C KCG  N D   D C  C   R                    +  WKC +C+ +N++ +  C +CG+  E  +LC  C S +  A    C  C      W CW   C  +N+   +YC  C   +     + I   + +SG     W+C+KC+ IN DY      +  C  +               RG  C YC       + KCP C
Sbjct:  552 WICTKCGFFNGDLGSDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKE--KLCNIC-SKRFQAAGEICPGC---NARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYNDYCWANGYCPGR---------------RGRLCYYCKCWVEHSQYKCPNC 742          
BLAST of SMED30032949 vs. Planmine SMEST
Match: SMESG000022822.1 (SMESG000022822.1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.105e-7
Identity = 67/269 (24.91%), Postives = 108/269 (40.15%), Query Frame = 3
Query:  930 DEFCMACLN--EKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSSMESEKCVICRNETNCNCKSNTKNSAITNENHKETLVENWENNKNNDASATNI---------NSDQWKCDKCGMLN--YDDFCSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKCKSIKIAADRRFCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKK----DIFSIKDISGM---FWLCKKCSAINDYEKKVCTSFSC 1676
            DE C  CL    ++ T  +   + N+++SG ++S      T     EN +    E  +   NE + +  +NT    + ++  KE  + N      N+ + TN+         N D+W C+ C  LN      C  CLN  R   +W C  C  +N      C +C K +++++              RF          W CW   C +LN+P   +C  C  QR       DI   +  +G    FW+C+KC   N+   + C   +C
Sbjct:  132 DETCSVCLALVSEMATKSRNNNDHNIDRSG-ESSFSGNEKTIMKNNENRNEEGKEMPLTTENE-DYSTNNNTSKKILEHDVGKENPIRN----DKNEENVTNLKSFHKLLIDNRDKWLCEMCQCLNPFQSADCQGCLNSCRDKCIWMCSWCDLINHINTSICSACEKRDKSVE--------------RFL--------MWKCWKPHCEHLNNPDCKFCKKCFLQRDAPKSLSDIIERQMGNGRILKFWICEKCWKYNNENFRKCEGKNC 372          

HSP 2 Score: 54.6842 bits (130), Expect = 1.881e-7
Identity = 134/549 (24.41%), Postives = 215/549 (39.16%), Query Frame = 3
Query:  138 EENFRNILNVEDIL-YANRSVMSNLQISIDEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKCKLLEIGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITDLLIGSICCFCEMRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSF------IYQNVDKVPNFDPITHLNGANYDYKITYKPEKNSYENI----NEIQNAANRKEKSTENLKINEENIVKSSKDFSEWKCSKCGLLN--YDEFCMACLN---EKVITNQKYGQEINLEKSGAKNSVEKRNTTSTYRYENVSSMESEKC--VICRNETNCNCKSNTKNSAITNENHKETLVENWENNKNNDASATNINSDQWKCDKCGMLNYDDF--CSA--CLNERRTIKLWKCERCKKLNDFFIEKCFSCGKT--NENIQLCKKCKSIKIAADRRFCDTC-YNNAPH---WSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFS--IKDISGM----FWLCKKCSAINDYEKKVCTSFSCKQ 1682
            E N +N L V+D++    +  + NL +   E  +Y +D LY E  N +KK Q+  N Y+  + Y+ C L +        + K   +    EI    I   I +FVK+   I++ E +       E  +++I   C +CE  ++     C+VC AL  +M   + N+ +        +  ++SF      I +N +          L   N DY       K   E+     N I+N  N  E++  NLK   + ++ +     +W C  C  LN      C  CLN   +K I    +   IN   +   ++ EKR+ +       V      KC    C +  N +CK   K       +  ++L +  E    N           W C+KC   N ++F  C    C + R    +       ++N   IE   S  K   N N   C+KC  +    D + C  C + +  H   W CW   C   N   + +C  CK  R+  D  +  +K  +G      W+C+KC   N  E   C   +C Q
Sbjct:    6 EYNPQNFLVVDDLIELLMKKKLENLSLYFIE--SYDSDKLYSEFYNLKKKLQLYFNHYNNNELYKSCLLKD--------DEKFTSVHPDGEIQ---ICHIIDDFVKHFCTIWKMETR------NEKQEMIIPENCFYCENPIESDDETCSVCLALVSEMATKSRNNNDH----NIDRSGESSFSGNEKTIMKNNENRNEEGKEMPLTTENEDYSTNNNTSKKILEHDVGKENPIRNDKN--EENVTNLKSFHKLLIDNR---DKWLCEMCQCLNPFQSADCQGCLNSCRDKCIWMCSWCDLINHINTSICSACEKRDKS-------VERFLMWKCWKPHCEHLNNPDCKFCKK--CFLQRDAPKSLSDIIERQMGNGRILK-----FWICEKCWKYNNENFRKCEGKNCGHLRYENAVKWNATNSQINTGQIESQHSDNKITLNPNQWQCEKCTLVNENRDYK-CVVCNFPSQSHRSPWQCWKVNCGKKNKGEDVFCLQCKLDRNPPDPITKLVKSYAGKEIKEIWICEKCGNENRKENDKCDKKNCYQ 511          
The following BLAST results are available for this feature:
BLAST of SMED30032949 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032949 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022823.10.000e+098.53SMESG000022823.1[more]
SMESG000022833.13.840e-923.16SMESG000022833.1[more]
SMESG000022814.15.416e-824.22SMESG000022814.1[more]
SMESG000022780.11.081e-726.42SMESG000022780.1[more]
SMESG000022822.11.105e-724.91SMESG000022822.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032949 ID=SMED30032949|Name=SMED30032949|organism=Schmidtea mediterranea sexual|type=transcript|length=1884bp
ATTCTTAAAATACTGTGTTTAAATAATTCAAAAATCAAATTTATTTTCCG
AGAAAATTTTATTATTTAATATTTGTAGAAAATTGTAATTATCAAAAACA
TTAAATGACGAACATAAAGGCTTTATATTCTTACATCGAAGAGAATTTTA
GGAACATCTTAAACGTTGAAGATATTCTTTACGCCAATCGCTCAGTAATG
TCTAATCTACAAATTTCAATAGATGAGTATAATAATTATTCTACTGATAA
TTTATATTTGGAACTGAGAAATTGCCGAAAAAAAGCACAGATTTTACTTA
ATTTATATCACTGTCCTAAAGAATATGAAAAATGTAAACTCTTGGAAATT
GGTTCCCATTTAAATAATACGAATAATAAACCTTTCGGCATTGAAAATGT
TCAAGAGATAAATTCAGATATTATTGTTGAAGCTATCGGTGAATTTGTAA
AATATCATCACGTGATATATGAAAGAGAATTACAAGTTGAATTAGATAAA
AATAAAGAAATAACTGATTTATTAATAGGGAGTATTTGTTGTTTTTGTGA
AATGCGATTAGATCGTGGTCAAAGCTACTGTAACGTTTGTGAAGCTCTAA
AAGACGACATGACAAAATATGCAACAAATCATTATGAAACATTAATGTTG
GTAAAAACTCAACAACCGAAAAAGACATCCTTTATTTACCAAAATGTTGA
TAAAGTTCCAAATTTTGATCCTATCACTCATCTGAATGGAGCAAATTATG
ATTACAAAATCACTTACAAACCCGAAAAAAATTCTTACGAAAATATAAAT
GAAATACAAAATGCAGCAAATAGAAAAGAAAAATCTACTGAAAACTTGAA
AATAAATGAAGAAAACATTGTAAAAAGCAGTAAAGATTTCAGTGAGTGGA
AATGTAGCAAGTGTGGATTGTTAAATTACGATGAATTTTGCATGGCTTGT
TTAAATGAGAAGGTTATAACTAATCAAAAATATGGTCAAGAAATAAATTT
GGAGAAAAGTGGAGCAAAAAACTCCGTGGAAAAGCGGAACACCACTTCAA
CATACAGATATGAAAATGTCTCTTCAATGGAATCGGAAAAATGTGTTATT
TGTCGCAATGAAACAAATTGCAACTGCAAATCCAATACGAAAAACTCAGC
AATTACTAACGAAAACCATAAAGAAACTTTGGTAGAAAATTGGGAAAATA
ATAAAAATAATGATGCGAGTGCCACCAATATAAATTCTGATCAATGGAAA
TGTGACAAATGTGGAATGTTAAACTACGATGACTTTTGTTCGGCTTGTTT
GAATGAAAGGAGAACAATCAAATTATGGAAATGTGAAAGATGCAAGAAAT
TGAATGATTTTTTCATCGAAAAATGCTTTTCATGTGGTAAAACAAACGAG
AACATTCAACTGTGCAAGAAGTGCAAATCAATAAAAATCGCTGCTGATCG
GCGTTTCTGTGATACGTGTTATAATAACGCACCACATTGGAGTTGTTGGA
ATGAAAAATGCTCCTATTTAAACGATCCATTCAATAATTATTGTGCTATT
TGTAAATTTCAAAGGCATAAAAAAGATATTTTTTCCATCAAAGATATAAG
TGGAATGTTTTGGCTGTGTAAAAAATGCAGTGCAATCAATGATTATGAAA
AGAAAGTTTGCACGTCATTTTCATGTAAGCAAAAGTTCGATTTTACCACG
CACAAATGTGAAATATGCAGAAAGAAATTTAGAGGGAATTCGTGTAAATA
TTGCGAAACTAAATGTCCAACATGCTTTTACAATAGATGTGAAAAATGTT
CAATCTTACCAAAATTTAAAACCAATTGAAAATGTTTATAAAAATTGACT
TTTAAAAGTATTTTTACACAGAATATAAAATTTT
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protein sequence of SMED30032949-orf-1

>SMED30032949-orf-1 ID=SMED30032949-orf-1|Name=SMED30032949-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=575bp
MTNIKALYSYIEENFRNILNVEDILYANRSVMSNLQISIDEYNNYSTDNL
YLELRNCRKKAQILLNLYHCPKEYEKCKLLEIGSHLNNTNNKPFGIENVQ
EINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITDLLIGSICCFCEM
RLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDK
VPNFDPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKI
NEENIVKSSKDFSEWKCSKCGLLNYDEFCMACLNEKVITNQKYGQEINLE
KSGAKNSVEKRNTTSTYRYENVSSMESEKCVICRNETNCNCKSNTKNSAI
TNENHKETLVENWENNKNNDASATNINSDQWKCDKCGMLNYDDFCSACLN
ERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKKCKSIKIAADRR
FCDTCYNNAPHWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSIKDISG
MFWLCKKCSAINDYEKKVCTSFSCKQKFDFTTHKCEICRKKFRGNSCKYC
ETKCPTCFYNRCEKCSILPKFKTN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
IPR000082SEA_dom
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0007155cell adhesion
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 231..254
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 263..285
e-value: 0.49
score: 14.3
coord: 457..483
e-value: 0.58
score: 13.8
coord: 498..524
e-value: 0.97
score: 12.0
coord: 376..398
e-value: 1.3
score: 11.1
coord: 403..427
e-value: 0.65
score: 13.4
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 459..480
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 405..424