cilia- and flagella-associated protein 46
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032947 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16
Homology
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Human
Match: CFAP46 (cilia and flagella associated protein 46 [Source:HGNC Symbol;Acc:HGNC:25247]) HSP 1 Score: 238.424 bits (607), Expect = 8.744e-62 Identity = 192/759 (25.30%), Postives = 345/759 (45.45%), Query Frame = 2
Query: 914 NEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILD--HDLSMKEGENDKDVHGKFTK--TFSWENVVT--RINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPV------FLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQIT 3154
+E++ LL ELA+ SL + C++ +++M S++ L+ E + + E + ES + A ++ +L I++ L +R + R+I + CA+ WN CLP+LQ +L + L VAD LE + L L QVH+E+ + +++ A ++L+KA LD Y + I+ +L++ LY+ P D+A +EQ + + + K R LL G LAPD F ++LD ++ + G+N G+FT +W + V+ + +L R GN +DK ++++W +L K++RK +WD+CR AS F +LYD+ + K ++++ R+G + G D S+ + + E + Q ++ LL + E FI AEA + LR+EG +L P+DL +HP G++ + + EW +Y +E LS + +LR+A + ++Q W QNA Y+ N++ H++ LV + + ++ + ++ LA G+I ++P+ F+ N F SP LD A ++ K A+++C++ +++T+ P T+ T+ QLI WV+ KQLLQQ +G LGT++ + R + +++M + C + S+ ++ C+W + V L L +L
Sbjct: 286 SEEKMLLLFELARFSLTLKCMEISSACLSDLKKMESKDPGKLI-EMECLECESEALRLESKMKVYNRAAVEAQLDIIQRLDVALQRAVRLGDPRVIHVVCATQWNTCLPLLQHNLRHHLRKPLAGVADVLEKLDSLMTLLRCQVHMEMAQIEEDEDRLEPATEHLRKAARLDSLGLYRDRIQMASTRLRLCTTLYQAPERAEDKAIMAVEQAKKATPKD--SVRKKRALLVNAGLALAPDAFQIVLDSENEAKVSTGKN----RGRFTYLCAKAWHHTVSVDKAAGHLRRLGNENDKERIQIWAELAKVARKQGVWDVCRTASRFCLLYDNVKVK---KLRL----RRGKKKRG-----------------------RDGSVQDTWSQPE-------VVLQRQVCPDLLRKFAEVGFIHAEATVHLLRSEGVELNDRAIPPEDLSQHPAGYVPEPPEVNAEWITYRTWIESLSRCAMNNWLRSAEIGQEIQEAWIVQNAVVYVLNHNHHLILAGRQKELVDALYHLLSIVKATGHSGDPVMLVTLCNTLARGLIISWIPVQAAEKSRKFMRPNAFHSP-----LDAGA------------------------TSEIKTAVEVCEFALNLTNGSAP------------------------EETVPTGTRQQLIATWVKAKQLLQQQIGPRLGTEEQGTNEDVSSVTRVLVALEM-YSCNGLGLMDFTVPSLAQLVKMASECNWSDPLVELQTLTRLTHFA 946 HSP 2 Score: 129.798 bits (325), Expect = 6.219e-29 Identity = 260/1198 (21.70%), Postives = 469/1198 (39.15%), Query Frame = 2
Query: 4727 LPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIP-EYCAHVEIIKANLEEYKKQNKSQVLP-MNFETQSTLPLKLSHLN-------------RKLTKFGYIHETWIKIAWILVEIGYNQEA----SEVICVAEELSKMLND--------EKMNTECNLLKAKLMINQGE---------------FDEALLYIDAIGS----CHFTFKQWSEIIDLLIEIGTDQANE----HGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQ--IIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESK--EWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEH-SLNQQFLYCAFIDTKHKTANLSKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFP-----------PGNERNSPSNQKANKETISQPGK---------DSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTK-IIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQ--ITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKER 8074
LP S EEW+ + ++ L + + I P + D L++ L + LT P+L + IS D V +K L+ HLR + ++A HE+ + V E + + I E+ K+ + LP +N +T P ++ L+ R L + H W+ A +L+E+ Q A SE +EL + + + N E N +AK MI Q + EALL ++ G CH + ++I+ + ++ N I+ + R L ++ RVA+ + L+ +M+ GLL ++R+ +D G N +V ++ A + +L E K+ YL + + + + +++ L + E L Q + D+ V N P+ RKL R KL L + ++M E+ Q + L +++ +Y++ D S +W + + + L + + +I A+ L G + L AM+ P + + L KS + +++ K + E+ + + K +M+ + YL QA E++ Q + +++ D A+ +++ G D + Q L L Q+ + MR++LL S QLAA +LQ + +R R T SL V+++L + AW+ V+ LL ++ + +++F H S ++ LY A + K ++ K A+G K+ R+A SP+ F + + QT+ Q + E ALN + L++EE + + L LGP+ E L FP P +S ++ +KE + G+ D I+++ D L LE + SS+SR+FSLQ+L +RL V K+ +S+ P + R + KK K IP R +P + +D++ +F+VDP +++ + S +T +L Q T RW G +G P+ + E L + + E+F+S + L+ +N + Q D + ++ +R
Sbjct: 1417 LPMSIEEWASYSCPEEVLSVLKQDRSDSTVNPSSIQKPTYSLYF--LDHLVKALQKMCLHELTVPVLQLGVLIS--DSVVGSKGLSDLYHLRLAHACSELKLREAAARHEEAVGQVCVSELEQASCRKEIALKKEKNKEPLLEESLPALNEQTLPVQPGEIKPLDAKDKILKMNGETGRDLDGTSFPH-LWMLKAEVLLEMNLYQPARLLLSEAYLAFQELDEPCAEAQCLLLLAQLANKEKNYGQAKKMIAQAQHLGGSEEFWYNSTLTLAEALLSMEHSGREATVCHI----FQKLINAFKILKKERPNRLPLLEFMITDLEARCLSLR----VRVAQHSAVTEPTECSLLLKEMDDGLLEIERKF--IDCGCKENCVDV-KLERAKIKRLRAQNEKDEEQKTAYYLEAYGLAQGAVAEEEGRLHSIQGLYGLAQGAMAEEEGRLHSVQGLLSLQDLQNV---------NTPLMRKLARLKLGLVEMALDMLQFIWEEAHG---QQSEQGSLEKLLADYLQNT-SDYTSVGLQWFTLKRTLAHGALAQLGSLQPLSVGCVEIRARLLGLAGRALHLLAMQADPVHPTCYWEAGPSVGAKLSGLKSLELEVEEEGATKSSRDPPASRAAPEEHCRRGED------LKRRMVLAQQYLAQASEVLLQCLQVALGSGLLDVAAAASLEMVECVGTLDPATTCQFLALSQSCSASETMRDVLLAATANTSSSQLAA-LLQLQHQLRCQDR-----TTTSLGARVEQRLAAVSKAWQNLCVTEQHFNLLNEMP--PTFWILFLHLSGDRSRLYGAAYE---KPKFITAAKGKVQAVGGSCKVMRLAISPT----------------------------AFSHLLACAQQFRKQTQAQ-VYSEDMALNIGSEPEGLQVEEKERPV-QRLSSVLGPLEELLQPLFPLLSLSKARVQTPAVVADSGKSKGKDKERKTSTGQHSTVQPEVADKIVLVADRHLLELPLEGLSVFDEGTISSVSREFSLQMLWNRLHKEETEGGVK--------KEGRSRDPKK----RSLAKKGRKGSIP--RTIPPDCIIVDSDNFKFVVDPYEEAQGPEMLTPVS-----ITQDILERFQDTFTSRWAGHLGSKHFPSQAQWEQALGSCSGFFFYGMESFLSHILVERLVAMNLQECQVAVLLDLARSYQSLKR 2519 HSP 3 Score: 122.479 bits (306), Expect = 1.203e-26 Identity = 56/214 (26.17%), Postives = 120/214 (56.07%), Query Frame = 3
Query: 126 ATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDD 767
+ D+ V+ E LK ++ +C+++YF + Q+L RA+L +AQ+ P+ + + + +KAINF+K E R+++L+YN++++Y+++VR +L+P + +L SLS I L + + + ++R++LML L++C++ AG ++E + A F+ H P + + + ++ + + + KN ++ + LK +++D
Sbjct: 45 SPDLFVLCAEQALKMRQPEVSEDCIQMYFKVKAPITQFLGRAHLCRAQMCAPKSAENLEEFENCVTEYMKAINFAKGEPRYYFLVYNASVLYWQMVRPFLKPGYRHHLIPSLSQIINVLSQTEEEDKEWRAELMLELLECYLQAGRKEEAARFCSTAAPFIKSHVPQKYRQIFSVMVRHELMDELQLKEEKKNSISLSVTFYINMLKAKAEQND 258 HSP 4 Score: 111.309 bits (277), Expect = 3.039e-23 Identity = 51/147 (34.69%), Postives = 86/147 (58.50%), Query Frame = 2
Query: 3743 EQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILL 4183
E DL +R ++ +F Q+ W LK LDEA+ LPRT H+L +F+H ++ K +L + +++QKFK + E+ +A +W +A+ S S +L Y +I++L+ KV+ +ME QWL H +P+E ++ +A+ ILL
Sbjct: 1104 EDDLALRAALYGLLFHSHADQDDWEGGLKVLDEAVQVLPRTAHRLLIFKHMVIVKAKLGQNFSMEIQKFKAESEDYLARMWHRLALNSPSVSGELACYNNAIQALQKPEMEWQKVEYLMEFGQWLHHRHFPLEDVVFHLRWAVEILL 1250
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Human
Match: CFAP46 (cilia and flagella associated protein 46 [Source:HGNC Symbol;Acc:HGNC:25247]) HSP 1 Score: 120.553 bits (301), Expect = 7.667e-28 Identity = 51/181 (28.18%), Postives = 108/181 (59.67%), Query Frame = 3
Query: 126 ATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSI 668
+ D+ V+ E LK ++ +C+++YF + Q+L RA+L +AQ+ P+ + + + +KAINF+K E R+++L+YN++++Y+++VR +L+P + +L SLS I L + + + ++R++LML L++C++ AG ++E + A F+ H P + + + ++ + +
Sbjct: 45 SPDLFVLCAEQALKMRQPEVSEDCIQMYFKVKAPITQFLGRAHLCRAQMCAPKSAENLEEFENCVTEYMKAINFAKGEPRYYFLVYNASVLYWQMVRPFLKPGYRHHLIPSLSQIINVLSQTEEEDKEWRAELMLELLECYLQAGRKEEAARFCSTAAPFIKSHVPQKYRQIFSVMVRHEL 225
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Xenopus
Match: ENSXETT00000034331.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:19360502:19399394:1 gene:ENSXETG00000006684.1 transcript:ENSXETT00000034331.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 157.147 bits (396), Expect = 1.344e-38 Identity = 125/476 (26.26%), Postives = 204/476 (42.86%), Query Frame = 2
Query: 1736 VTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKG 3163
V + +L R + + ++RLW DL K++RK ++WD+CR A F +LYDDGRW + +K + +G + NA+ A TN E+ LL L E FI AEA I L++E +L P P+D + HP G+ ++ +W Y + + +LS FL+AA L V+L W T NAA Y+ NY+ H+++ + D L ++ N ++ +++N LA G+I +++P SP ++ K KK G+ S +SV+ +C+Y++D+T+ P + I + Q++ WV+ KQLLQQ +G LGT++ E GQ + R + +++M F + S++ LC W + V L LL +LA K
Sbjct: 162 VQKTEGHLKRIEDKNAAERVRLWADLAKVARKQEVWDVCRAACRFCLLYDDGRW----------NIQKKDTCLGLEGELSNAK--AAFTN----------------------------------ERALLRTLAEIRFINAEATIHLLKSENCKLNEPPIPPEDTRMHPVGYTAVNPEDNPDWIVYRDWISELSRYATENFLQAAELGVELHEGWITHNAAVYILNYNKHLIS-------TERLSELTDALHKL-NTVLLAMLSN---ALAKGLILRWIP-------GSAASPKDASIHSAKVKK-------------APGKGSEKSVH-------VCEYVLDLTNGRMP------------------------EEMVPISVQQQILATWVKSKQLLQQQIGPKLGTEE-ESNEGQNLMTRVVIAIEMHSCNGLGLMDF-SVPSLSQVFDMTCLCHWADPFVELQLLTRLAHFAYKA 527 HSP 2 Score: 96.2857 bits (238), Expect = 3.516e-19 Identity = 44/116 (37.93%), Postives = 81/116 (69.83%), Query Frame = 3
Query: 186 ANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLL 533
+ +CL+LYF +P +NQ+ RAYL +AQL P+ L+K + + LKAI F K+++R+++L++N+++IY+++V+ +L+ L SLSS+ K L+E+++ + +R+QLM+L
Sbjct: 7 SKDCLQLYFRNNPPQNQFFGRAYLCKAQLHIPQ----SDQLEKSVVYYLKAIGFGKQQQRYYFLVFNASVIYWQMVQPFLKSGSRHLLIPSLSSVVKALDEIDERDHTWRAQLMIL 118
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Xenopus
Match: fen1 (flap structure-specific endonuclease 1 [Source:Xenbase;Acc:XB-GENE-955518]) HSP 1 Score: 60.8474 bits (146), Expect = 2.982e-9 Identity = 43/198 (21.72%), Postives = 76/198 (38.38%), Query Frame = 2
Query: 7856 QITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIR----------------NPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAV 8401
Q TP W G++G P+ E E + ++ ++ E F++ L +N + Q + D + T ++ R + L + A L+S G++S + NQW ++ N + L G G+ + +R P E L++ P+ + VLYGLPN+ V
Sbjct: 10 QFTPHWEGIIGSSHIPSHAEWEKLTTDCSAFLFYGMERFLAHILSDKFLALNLKECQLMILLDLVRTSESFARQSKVDVQKRATSLTLERPVETALLLSATGIRSVMLNQWHTTLEKNAKRLDCLAENLLATGKTTGQSVHSLRRLQWNSNLLNDEGDNIKPDDGEEEILMREPVSKPPAWDPSVFSYVLYGLPNMVV 207
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Mouse
Match: Cfap46 (cilia and flagella associated protein 46 [Source:MGI Symbol;Acc:MGI:2444387]) HSP 1 Score: 301.212 bits (770), Expect = 3.859e-81 Identity = 273/1116 (24.46%), Postives = 497/1116 (44.53%), Query Frame = 2
Query: 917 EQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVH-GKFTKTFSWENV--------VTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSS---FQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEF---YKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQNTSVL----------MQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLFLEPHTXXXXXXXXXXXXXKLP-HILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLN 4186
E++ LL EL ++ L C+ +++M +E+ LL E + + EL + S + ++ +L I++ L IR N I + C + WNICLP+LQ +L + L +A+ LE + L L QVH+E+ +++ AI+++QKA+ LD Y + I+ +L++ LY+ P P D+A +EQ + + K R +L G LAPD F ++LD EN+ V GK F++ V + +L R G +DK ++ +W +L K++ + +WD+CR AS F +LYD+ + K S+ ++ V MS+ +D ++++ ++ LL ++ + FI AEA + LR+EG QL P+DL +HP G++ + + EW +Y +E LS + ++RAA + +L W QNA Y+ N++ H++ LV S F ++ + + M+ ++ N LA G+I ++P T +K+KK+ + G ++ST ++ + A+++C++ +++T+ P + + LI WV+ KQLLQQ +G+ LGTD+ + R + +++M + C + P L+ V ++ C+W + V L L +L +++ + ++ A ++ + H+ + +N AKLA LC+ Q S++ + + V++ + N LV + ++W L+ +N + ++ II+ ++ T +KK+ E ++ LP H D Q T + FL E DLT+R ++ +F + W LK LDEA+ LPRT H+L +F+H ++ K +L + +++QKFK++KE +A++W +A S + +L Y+ +I++L+ NN KVD IME S+W+++ +P++ ++D+AI ILL+
Sbjct: 287 EEKILLLFELGRLCLTLKNEPIASDCLQYLKKMETEDTGKLL-EMEGLECELDALRLGSKLKMYLRGAVEEQLLIIKRMDNILQRAIRLGNPTTIHVLCTAQWNICLPLLQHNLRHHLRKPLTNIAEILEKVDSLMILLRCQVHMEMAYIEQDDDRVEPAIEHMQKAMRLDSLGIYRDKIKITYRRLQLGTMLYQSPDRPEDKATMAIEQAKKATPKD--SARKKRAILVNAGLALAPDTFQIVLD-------SENESKVSIGKIKGRFTYLCAKARHHILSVDKAAGHLRRLGYENDKDRIVIWAELAKVAWQQDVWDVCRTASRFCLLYDNVKAKR-------------------STKLKRGRKKKVIEGSMSDWAPSDMAMHK------------------QMPPDLLRKIAKVLFISAEATVHLLRSEGVQLNNQAIPPEDLSQHPAGYVPEPPESNSEWITYRTWIENLSQYAMNNWMRAAEIGQELGESWIVQNAVVYVLNHNRHLILAGRQKELVESLFHFLSIIKIVGYTEDSVMLVMLCN---ALARGLIMSWIP----------------TQTPEKSKKN-----VRPTLVHGPLESST--LSDIRAAVEVCEFALNLTNGNVP------------------------EDIVPTNVRQHLIATWVKAKQLLQQQIGQRLGTDEQSTNEDISSVTRVLVALEM-YSCNGLGLMDFTVPPLVQV---VKMASECNWSDHLVELQTLTRLTHFAY---MAHDHEVTMACSQNATQMGLK-HLRSFEPSN----------AKLAAEM------LCMVSCIQGRSIMENLKGRKQLRLAAAKAFVESARFGGLANSNSLVMMAARHYWNTWLPLLSSPANRKKAKASLQRIISIINKTENKKQ------------------ETDKTLPLHQWPSADFQTGGT--GPEIPFLPGA---------EDDLTLRAALYTLLFNSHADRNDWEMGLKVLDEAVQVLPRTAHRLLIFKHMVIVKAKLGQNFTMEIQKFKDEKESYLASMWYRLAQNSKTVCGELSCYQNAIQALQKPENNWQKVDYIMEFSEWMYYKQFPLDDVFDHLDWAIEILLS 1252 HSP 2 Score: 191.815 bits (486), Expect = 7.512e-48 Identity = 278/1277 (21.77%), Postives = 523/1277 (40.96%), Query Frame = 2
Query: 4715 PADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSP-FVIPEYCAHVEIIKANLEEYKKQNKSQVLPMNFETQSTLP--------------LKLSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRL------RLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVI-EKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYG---HVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPI-----FEKLGNFFPP--GNERNSPSNQKANKETI--SQPGKDSIIILTDEILSVASLESIPKLK--SSISRDFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQ--ITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIR---NPPI--EEVPELIKNLSLTS-----PSESFIKSNLVLYGLPNIAV 8401
P D +PTS EW+ + S+D L + P + D L + L + L+ PIL + + ++ V +K L HLR LQ + A HHE+ I F E + + I E+ K+ LP E Q TL LK++ K + + W+ +L+E+ +Q A ++ A+ + L D + LL A+L + +A+L ++ + W + L E + +IS L +++ NRV+ ++ +L + + ++ + L D ++ L E+E +K +++ ++ +I + + K+ ++ + +K + + +F I + S + N P+ R+L R KL L++ +++ + ++ + ++ + + L + D +W + + + + L + +C DI AQ L+ G H++ ++ +YP+ W L+ + T+K L +L+ + ++ +E +S + + E+ S K K +M+ R +L Q E++ Q I S+++ D V +A+ +++ G D + Q L L Q+ T+ MR +LL + QLAA +LQ +R+ + + SL+ SV++KL S+F AW+ V+ +L ++ + L +HS ++ LY A + + + P K +G K+ R+ATSP+ I DL + F + +T++ EQ + ++ S I+E++++YL PI F + N P G+ + +K K ++ QP + +I++ D+ L LE + L +S+SRDFSLQ+L +RL + + KK + KK+ K I + R +P ID++ ++F+VDP +++ + E+T +L + + T RW+G +G+ P+ + E L + E F+S + L+ +N + Q + D + +++R ++ S S A L+SL+GV+S V NQW L+ N + L G +G+ ++ I N P +E P++ KN L+ P N VLYGLP+ A+
Sbjct: 1464 PPDEIPTSIGEWASYSCSEDLLHVFKQDKSDCTINSSSFQKPTYSLYY--IDHLAKALQKMFLHELSIPILQL--GVLIAEAVVESKSLADLYHLRLLQVCSDLRLHYAATHHEEVIGQAYFGDMEQASCRKEIAFKKEKNKEPEMEDYLPTLHE-QLTLSDSVEVKALIAQDKILKINGETGKGLEGTSYPQLWLLKGEVLLELELHQPARLLLSEAQWAFQELGDICSEAQSLLLLAELANKEKNHAQAMLMVERAQQLGGAEEFWYKSTLTLAEAILSSEDRRKEISVCSLFQKLIDTFRALQNERPNRVSLLEFLIMELEARCVKLRIQFACNLTDEEFNEHPF-----------LLRELEDHLLEVEQNLIFGGYYKNYVDLKLDHAKIKRLCAQKEKDEEQKTAYYLEMYSLSQKAVSEEEEVFHRIQALLSLHDLQNINSPVMRRLARLKLSLAETCLDLLQLVCKEALDLQMEQGSFEKL--LADFLQNTTDYTSIGLQWFFLKRTLPHLVLTQLESLQPLCVGCTDIRAQLLILAGKALHLLYVQTDPVYPS--FCWEEELL-----VGTKKDSLRSLETDTEDRSVETSSSAVFKEAPKEQ----SRKAEGLKKRMVLARGFLAQTSEVLLQCIQTSLSNNLLDIVASASIEMVECIGILDPITTCQFLTLSQSCSTSEMMRKVLLTATKNTGSSQLAA-LLQLHQRLRQQDKMST-----SLFASVEQKLASSFRAWQSLCVTEQHFSILNEIPPVFR-ILFLQHSQDRSCLYGAAFE---RPKTMPSPKGKLITVGGYCKVTRVATSPTAISDLLTSIQWF-----------QRQTRVKEQAL--------------------------------CTQSLSSILEHMEDYLKPITPQLTFPESRNQMPTAVGDAGKNKGREKERKISLPSGQPDPEYLILIVDKFLLELPLEGLSMLNEVTSLSRDFSLQMLWNRLHKEEREGSMKKEVKSKDLKK------------KTPGKKALKSI-MTRIIPPECNLIDSDTIKFVVDPYEEARGTELMR-----PIEITQEILEKYRDTFTGRWMGHLGNHNFPSQADWEQLLDSCRGFFFYGMETFLSHLEIERLVAMNLQECQMMILLDLTRSSHSQKRTIESSEGQSASQRSLVKPIQTAILLSLVGVESIVDNQWPTLMQDNMLRATILWDNLLAIGKPLGKTVRLIHKLCNEPGSQDEKPQVSKNEPLSELQPHHPESLTTALNWVLYGLPHQAI 2640 HSP 3 Score: 113.235 bits (282), Expect = 5.409e-24 Identity = 53/188 (28.19%), Postives = 111/188 (59.04%), Query Frame = 3
Query: 93 SKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLI 656
S+ + E F + D+ V+ E LK I+ +C+++YF + Q+L RA+L +AQL P+ + + + +KAINF+K E R+++L++N++++Y+ +VR +L+P ++ + SLS I L + + + ++R++LML L++C++ AG +++ + A F+ P+ + + +T++
Sbjct: 36 SELDPTESF--SPDLFVLCAEQALKMKEPEISEDCIQMYFKVKAPITQFLGRAHLCRAQLCAPQSEENVEEFENCVTQYMKAINFAKGEPRYYFLVFNASVLYWNMVRPFLKPGYHHLVIPSLSQIITVLNQTEEEDKEWRAELMLELLECYLQAGRKEDATKFCLTAAPFVRTQVPHKYRQMFSTMV 221
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Mouse
Match: Cfap46 (cilia and flagella associated protein 46 [Source:MGI Symbol;Acc:MGI:2444387]) HSP 1 Score: 135.576 bits (340), Expect = 1.244e-33 Identity = 97/362 (26.80%), Postives = 166/362 (45.86%), Query Frame = 2
Query: 1406 LQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVH-GKFTKTFSWENV--------VTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSF 2464
+QKA+ LD Y + I+ +L++ LY+ P P D+A +EQ + + K R +L G LAPD F ++LD EN+ V GK F++ V + +L R G +DK ++ +W +L K++ + +WD+CR AS F +LYD+ +K + S++ ++ V MS+ +D ++++ ++ LL ++ + FI AEA + LR+EG QL P+DL +HP G++ + + EW +Y +E LS + ++RAA + +L W QNA Y+ N++ H++ LV S
Sbjct: 1 MQKAMRLDSLGIYRDKIKITYRRLQLGTMLYQSPDRPEDKATMAIEQAKKATPKD--SARKKRAILVNAGLALAPDTFQIVLD-------SENESKVSIGKIKGRFTYLCAKARHHILSVDKAAGHLRRLGYENDKDRIVIWAELAKVAWQQDVWDVCRTASRFCLLYDN--------VKAKRSTK-----------LKRGRKKKVIEGSMSDWAPSDMAMHK------------------QMPPDLLRKIAKVLFISAEATVHLLRSEGVQLNNQAIPPEDLSQHPAGYVPEPPESNSEWITYRTWIENLSQYAMNNWMRAAEIGQELGESWIVQNAVVYVLNHNRHLILAGRQKELVESL 316
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Mouse
Match: Cfap46 (cilia and flagella associated protein 46 [Source:MGI Symbol;Acc:MGI:2444387]) HSP 1 Score: 121.709 bits (304), Expect = 3.491e-27 Identity = 98/343 (28.57%), Postives = 167/343 (48.69%), Query Frame = 2
Query: 917 EQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVH-GKFTKTFSWENV--------VTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIK 1918
E++ LL EL ++ L C+ +++M +E+ LL E + + EL + S + ++ +L I++ L IR N I + C + WNICLP+LQ +L + L +A+ LE + L L QVH+E+ +++ AI+++QKA+ LD Y + I+ +L++ LY+ P P D+A +EQ + + K R +L G LAPD F ++LD EN+ V GK F++ V + +L R G +DK ++ +W +L K++ + +WD+CR AS F +LYD+ + K ++K
Sbjct: 287 EEKILLLFELGRLCLTLKNEPIASDCLQYLKKMETEDTGKLL-EMEGLECELDALRLGSKLKMYLRGAVEEQLLIIKRMDNILQRAIRLGNPTTIHVLCTAQWNICLPLLQHNLRHHLRKPLTNIAEILEKVDSLMILLRCQVHMEMAYIEQDDDRVEPAIEHMQKAMRLDSLGIYRDKIKITYRRLQLGTMLYQSPDRPEDKATMAIEQAKKATPKD--SARKKRAILVNAGLALAPDTFQIVLD-------SENESKVSIGKIKGRFTYLCAKARHHILSVDKAAGHLRRLGYENDKDRIVIWAELAKVAWQQDVWDVCRTASRFCLLYDNVKAKRSTKLK 619 HSP 2 Score: 112.464 bits (280), Expect = 2.555e-24 Identity = 50/181 (27.62%), Postives = 107/181 (59.12%), Query Frame = 3
Query: 126 ATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSI 668
+ D+ V+ E LK I+ +C+++YF + Q+L RA+L +AQL P+ + + + +KAINF+K E R+++L++N++++Y+ +VR +L+P ++ + SLS I L + + + ++R++LML L++C++ AG +++ + A F+ P+ + + +T++ +
Sbjct: 45 SPDLFVLCAEQALKMKEPEISEDCIQMYFKVKAPITQFLGRAHLCRAQLCAPQSEENVEEFENCVTQYMKAINFAKGEPRYYFLVFNASVLYWNMVRPFLKPGYHHLVIPSLSQIITVLNQTEEEDKEWRAELMLELLECYLQAGRKEDATKFCLTAAPFVRTQVPHKYRQMFSTMVRYEV 225
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Mouse
Match: Cfap46 (cilia and flagella associated protein 46 [Source:MGI Symbol;Acc:MGI:2444387]) HSP 1 Score: 108.227 bits (269), Expect = 1.908e-25 Identity = 46/162 (28.40%), Postives = 100/162 (61.73%), Query Frame = 3
Query: 183 IANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSI 668
I+ +C+++YF + Q+L RA+L +AQL P+ +E + + +KAINF+K E R+++L++N++++Y+ +VR +L+P ++ + SLS I L + + + ++R++LML L++C++ AG +++ + A F+ P+ + + +T++ +
Sbjct: 6 ISEDCIQMYFKVKAPITQFLGRAHLCRAQLCAPQS-EENEEFENCVTQYMKAINFAKGEPRYYFLVFNASVLYWNMVRPFLKPGYHHLVIPSLSQIITVLNQTEEEDKEWRAELMLELLECYLQAGRKEDATKFCLTAAPFVRTQVPHKYRQMFSTMVRYEV 166
BLAST of cilia- and flagella-associated protein 46 vs. UniProt/SwissProt
Match: sp|Q8IYW2|CFA46_HUMAN (Cilia- and flagella-associated protein 46 OS=Homo sapiens OX=9606 GN=CFAP46 PE=2 SV=3) HSP 1 Score: 238.424 bits (607), Expect = 4.199e-61 Identity = 192/759 (25.30%), Postives = 345/759 (45.45%), Query Frame = 2
Query: 914 NEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILD--HDLSMKEGENDKDVHGKFTK--TFSWENVVT--RINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPV------FLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQIT 3154
+E++ LL ELA+ SL + C++ +++M S++ L+ E + + E + ES + A ++ +L I++ L +R + R+I + CA+ WN CLP+LQ +L + L VAD LE + L L QVH+E+ + +++ A ++L+KA LD Y + I+ +L++ LY+ P D+A +EQ + + + K R LL G LAPD F ++LD ++ + G+N G+FT +W + V+ + +L R GN +DK ++++W +L K++RK +WD+CR AS F +LYD+ + K ++++ R+G + G D S+ + + E + Q ++ LL + E FI AEA + LR+EG +L P+DL +HP G++ + + EW +Y +E LS + +LR+A + ++Q W QNA Y+ N++ H++ LV + + ++ + ++ LA G+I ++P+ F+ N F SP LD A ++ K A+++C++ +++T+ P T+ T+ QLI WV+ KQLLQQ +G LGT++ + R + +++M + C + S+ ++ C+W + V L L +L
Sbjct: 286 SEEKMLLLFELARFSLTLKCMEISSACLSDLKKMESKDPGKLI-EMECLECESEALRLESKMKVYNRAAVEAQLDIIQRLDVALQRAVRLGDPRVIHVVCATQWNTCLPLLQHNLRHHLRKPLAGVADVLEKLDSLMTLLRCQVHMEMAQIEEDEDRLEPATEHLRKAARLDSLGLYRDRIQMASTRLRLCTTLYQAPERAEDKAIMAVEQAKKATPKD--SVRKKRALLVNAGLALAPDAFQIVLDSENEAKVSTGKN----RGRFTYLCAKAWHHTVSVDKAAGHLRRLGNENDKERIQIWAELAKVARKQGVWDVCRTASRFCLLYDNVKVK---KLRL----RRGKKKRG-----------------------RDGSVQDTWSQPE-------VVLQRQVCPDLLRKFAEVGFIHAEATVHLLRSEGVELNDRAIPPEDLSQHPAGYVPEPPEVNAEWITYRTWIESLSRCAMNNWLRSAEIGQEIQEAWIVQNAVVYVLNHNHHLILAGRQKELVDALYHLLSIVKATGHSGDPVMLVTLCNTLARGLIISWIPVQAAEKSRKFMRPNAFHSP-----LDAGA------------------------TSEIKTAVEVCEFALNLTNGSAP------------------------EETVPTGTRQQLIATWVKAKQLLQQQIGPRLGTEEQGTNEDVSSVTRVLVALEM-YSCNGLGLMDFTVPSLAQLVKMASECNWSDPLVELQTLTRLTHFA 946 HSP 2 Score: 129.798 bits (325), Expect = 2.987e-28 Identity = 260/1198 (21.70%), Postives = 469/1198 (39.15%), Query Frame = 2
Query: 4727 LPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIP-EYCAHVEIIKANLEEYKKQNKSQVLP-MNFETQSTLPLKLSHLN-------------RKLTKFGYIHETWIKIAWILVEIGYNQEA----SEVICVAEELSKMLND--------EKMNTECNLLKAKLMINQGE---------------FDEALLYIDAIGS----CHFTFKQWSEIIDLLIEIGTDQANE----HGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQ--IIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESK--EWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEH-SLNQQFLYCAFIDTKHKTANLSKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFP-----------PGNERNSPSNQKANKETISQPGK---------DSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTK-IIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQ--ITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKER 8074
LP S EEW+ + ++ L + + I P + D L++ L + LT P+L + IS D V +K L+ HLR + ++A HE+ + V E + + I E+ K+ + LP +N +T P ++ L+ R L + H W+ A +L+E+ Q A SE +EL + + + N E N +AK MI Q + EALL ++ G CH + ++I+ + ++ N I+ + R L ++ RVA+ + L+ +M+ GLL ++R+ +D G N +V ++ A + +L E K+ YL + + + + +++ L + E L Q + D+ V N P+ RKL R KL L + ++M E+ Q + L +++ +Y++ D S +W + + + L + + +I A+ L G + L AM+ P + + L KS + +++ K + E+ + + K +M+ + YL QA E++ Q + +++ D A+ +++ G D + Q L L Q+ + MR++LL S QLAA +LQ + +R R T SL V+++L + AW+ V+ LL ++ + +++F H S ++ LY A + K ++ K A+G K+ R+A SP+ F + + QT+ Q + E ALN + L++EE + + L LGP+ E L FP P +S ++ +KE + G+ D I+++ D L LE + SS+SR+FSLQ+L +RL V K+ +S+ P + R + KK K IP R +P + +D++ +F+VDP +++ + S +T +L Q T RW G +G P+ + E L + + E+F+S + L+ +N + Q D + ++ +R
Sbjct: 1417 LPMSIEEWASYSCPEEVLSVLKQDRSDSTVNPSSIQKPTYSLYF--LDHLVKALQKMCLHELTVPVLQLGVLIS--DSVVGSKGLSDLYHLRLAHACSELKLREAAARHEEAVGQVCVSELEQASCRKEIALKKEKNKEPLLEESLPALNEQTLPVQPGEIKPLDAKDKILKMNGETGRDLDGTSFPH-LWMLKAEVLLEMNLYQPARLLLSEAYLAFQELDEPCAEAQCLLLLAQLANKEKNYGQAKKMIAQAQHLGGSEEFWYNSTLTLAEALLSMEHSGREATVCHI----FQKLINAFKILKKERPNRLPLLEFMITDLEARCLSLR----VRVAQHSAVTEPTECSLLLKEMDDGLLEIERKF--IDCGCKENCVDV-KLERAKIKRLRAQNEKDEEQKTAYYLEAYGLAQGAVAEEEGRLHSIQGLYGLAQGAMAEEEGRLHSVQGLLSLQDLQNV---------NTPLMRKLARLKLGLVEMALDMLQFIWEEAHG---QQSEQGSLEKLLADYLQNT-SDYTSVGLQWFTLKRTLAHGALAQLGSLQPLSVGCVEIRARLLGLAGRALHLLAMQADPVHPTCYWEAGPSVGAKLSGLKSLELEVEEEGATKSSRDPPASRAAPEEHCRRGED------LKRRMVLAQQYLAQASEVLLQCLQVALGSGLLDVAAAASLEMVECVGTLDPATTCQFLALSQSCSASETMRDVLLAATANTSSSQLAA-LLQLQHQLRCQDR-----TTTSLGARVEQRLAAVSKAWQNLCVTEQHFNLLNEMP--PTFWILFLHLSGDRSRLYGAAYE---KPKFITAAKGKVQAVGGSCKVMRLAISPT----------------------------AFSHLLACAQQFRKQTQAQ-VYSEDMALNIGSEPEGLQVEEKERPV-QRLSSVLGPLEELLQPLFPLLSLSKARVQTPAVVADSGKSKGKDKERKTSTGQHSTVQPEVADKIVLVADRHLLELPLEGLSVFDEGTISSVSREFSLQMLWNRLHKEETEGGVK--------KEGRSRDPKK----RSLAKKGRKGSIP--RTIPPDCIIVDSDNFKFVVDPYEEAQGPEMLTPVS-----ITQDILERFQDTFTSRWAGHLGSKHFPSQAQWEQALGSCSGFFFYGMESFLSHILVERLVAMNLQECQVAVLLDLARSYQSLKR 2519 HSP 3 Score: 122.479 bits (306), Expect = 5.779e-26 Identity = 56/214 (26.17%), Postives = 120/214 (56.07%), Query Frame = 3
Query: 126 ATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDD 767
+ D+ V+ E LK ++ +C+++YF + Q+L RA+L +AQ+ P+ + + + +KAINF+K E R+++L+YN++++Y+++VR +L+P + +L SLS I L + + + ++R++LML L++C++ AG ++E + A F+ H P + + + ++ + + + KN ++ + LK +++D
Sbjct: 45 SPDLFVLCAEQALKMRQPEVSEDCIQMYFKVKAPITQFLGRAHLCRAQMCAPKSAENLEEFENCVTEYMKAINFAKGEPRYYFLVYNASVLYWQMVRPFLKPGYRHHLIPSLSQIINVLSQTEEEDKEWRAELMLELLECYLQAGRKEEAARFCSTAAPFIKSHVPQKYRQIFSVMVRHELMDELQLKEEKKNSISLSVTFYINMLKAKAEQND 258 HSP 4 Score: 111.309 bits (277), Expect = 1.459e-22 Identity = 51/147 (34.69%), Postives = 86/147 (58.50%), Query Frame = 2
Query: 3743 EQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILL 4183
E DL +R ++ +F Q+ W LK LDEA+ LPRT H+L +F+H ++ K +L + +++QKFK + E+ +A +W +A+ S S +L Y +I++L+ KV+ +ME QWL H +P+E ++ +A+ ILL
Sbjct: 1104 EDDLALRAALYGLLFHSHADQDDWEGGLKVLDEAVQVLPRTAHRLLIFKHMVIVKAKLGQNFSMEIQKFKAESEDYLARMWHRLALNSPSVSGELACYNNAIQALQKPEMEWQKVEYLMEFGQWLHHRHFPLEDVVFHLRWAVEILL 1250
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Match: V4CMX4 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_237561 PE=4 SV=1) HSP 1 Score: 753.436 bits (1944), Expect = 0.000e+0 Identity = 659/2653 (24.84%), Postives = 1195/2653 (45.04%), Query Frame = 2
Query: 1046 EADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHGKFTK----TFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKN------------------HFQ-IKIEN-----------SSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNST-----ESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLV-KITEKAVKVSPETHIGVVKRANKTNTNTGTVDAK-LALSFIYFCHGLCLHKTGQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLFLEPHTXXXXXXXXXXXXXKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNTE----------ATKEKSLSHPNSSVKNRSRNNFRKPGSG--LKP-----NVKNENIQETPEMXXXXXXXXXXXXXXXPTWFFLQEF------------RPR----TLEDICDIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMK-STGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIPEYCA------------HVEI------IKANLEEYKKQNKSQVLPMNFETQSTLPLK------LSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAI-----GSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLD---LGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIE---ISMEKLEIFDFLMKVNEKTNFK-SLLIKKLIVIEKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKE-SKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFID-TKHKTA--NLSKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFPPGNERNSPSNQKAN-----KETISQPGKDSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRL--KGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGS-----------------------FFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNP---------------------PI---------------------------EEVPELIKNLSLTSPSESFIKS---NLVLYGLPNIAVS 8404
E +F++ E+++ E ++ RL ++++C+ ++N R + ++++GC + WN+ LP+ QP+L I HL ++A++LE I L +L Q+H E+ EQI A+++++KA+ LD + Y E +E +H+L+++ +LY++P D A ++EQ R N + I R LL + G L+PD FL++LD + ++ + K TK + V ++ ++ R GN +D+ + RLW DL K +RK ++WD+CRVA+ F +LYDD RWK+ HFQ + + N S K +R GA+S ++ TS + + + D S+ L N K LL L E FI EA+I LR+E +L P P+D +K PKG++ ED +W YC+ ++ LS FLR L V L W +AA Y+WNY+ H+L + H ++ + + D LRQ+ + ++ N LA+ +I ++P P P S + D+K K + P TVG + T E+ KKA+++C+Y + +T+ + T I + I + L++ WV KQ+ Q + + LGTDD GQR + +A+ + +M + +N +F D +++ + Q ++ C W E + L L KL + S ++LV K +EKA++ + +G + K + + V+ + L+ + L ++ G+N S+ Q ++ + + YAR+ +Y LV + ++W L+ + I + + +++ C++ T D + + E + I+ I D K + K+ L ++ DL++R ++ +FQ + + +W E++ ++D+AI D+PRT H+L +F+HR++ K RL +++D+QKFK++ E+ VA +WR +A+ S QL +Y+ +IE+L + +N LK+D ++E SQWL+ + +PIE +++A++ILLN + + +LS N K + R + +KP + KP ++K+E + E+ + + T FF+ + PR T+ED+ DI+ L A++ G +D L+ A + R+WQV++++ +KE+ K V T++ KS + + + PKRKGP D LP S EEW+ +++ D+ LEA + K H ++ P + + L E L GY L+ P+L+ D ++R K ++ H++ L+ ++ + HEK + + + E A V++ +K +L + K + + +T ++ P K + HL + L + E W A +L+ GYNQ A E + A ++ D+K+ + + +L N+ ++ + + G F FK II+ ++ D N++ ++ L MK+ N++ E + N + + + L + ++AT+ L V ++M+ L E+ E+ E Y E + ++ + + + K + L++ L V+++ + I + +F D + + +S + N P+ R+L ++ + LI++ + ++ + L K + +V++Y+ E K+W + + ++ L + H++ I + Q LL G + L L+PN +QW M+ + S+L QD + K I E+T++ + S ++ + + +R YL QA E + Q++ +S+ H + +A+ ++++ GQFD + Q L LHQ+ T+ + ++L + +L+A + QR+ ++ ++NL+ ++ SV L + AWK +V N LELLK+ F++ +HS ++ FLY A +D KH + + +++ R KI + TSP +E+L +R K +++L E + R + I+N + E + A Q+ + E + +++ ++ YL P+ L N +SP+ AN K+ + P + II+L D +L LE++ L+ SS+SRDFSLQ+ HR + + DP K + + R+ R+ +K K++PL+R + +DT R ++ + + G+ F + +T EQQ TPRW+GL GDD P+ E E+YLKE++S I+ E +S P L +N D L C D +T K+ R + S DL + +A L SL G++ + NQW + N ++ F L+ G G+ ++ + +P P E+VPE + SES IKS NLV YGLPN+ ++
Sbjct: 391 ELEFLDCEMMVKVLGEKQESYQKVSVDTRLLSMKRCEEAIMNSKRQGDPNIVQVGCVTQWNLSLPLQQPNLRHHIRKHLTVIAEALEEINSLLVQLRCQIHTELSHCEEDSEQIQVAMQHIKKALSLDDGEVYRERLEVALHRLELRSELYQQPERAEDLAAMIIEQAR---NCDSGTIRMKRSLLVKAGEALSPDAFLLVLDSESETRDIASGKGPQTMITKLACKAQQFNKCVKKVEGHIKRLGNENDRERARLWADLAKTARKHEVWDVCRVAARFTLLYDDDRWKSVSEDRLSLIIEAQYLTKQHFQRVHMANTYYLVFSPVTVSREKESREDGATSEIEKKSTSRIGSRPTT----PDQSL----------PLYN---------KDLLRILAEVNFILGEAYIHLLRSEKVELNNVPIPPEDTRKRPKGYVAKKPEEDPDWLEYCDWIKMLSEMSTKSFLRGLELGVVLDEAWLVCSAAAYIWNYNNHVLAGNRHREILDTLSAVLDGLRQVGHAGETVMLVNICNALAYSLILPWIPAPA---PKEVQEPPTSPSPDEKGKDKK--PVRATVGTAKSKGGVTVTICSEAQPDLKKAIEVCEYGIQVTNG---------------------DNTRDI---VPISIRLPLLQTWVCVKQMALQQIPKTLGTDDETNMEGQRGMTKAIVATEMSALSKNGIMEFKDGINLTEITQLVENCRWTEKFIELQLWSKLTFL----SFDQKQHSLVLKCSEKALRFAV---VGTQPKNRKMDPHRAMVEQEMLSYTSCILGQSLMVNMAGKN-SIRRQAMEAFLNSARYARNAKNYGLVMSAARHYWNACVPLLNQPIERELIKEPVRVLLQCITATADSPK-------------KEDKEEKTGQGEGIVNIKGDNK----EEEKSSRLVG--------GIDDDLSLRAALYGLLFQAYADRGEWEESMVAMDQAITDMPRTKHRLLIFKHRVMVKARLGQDVQMDIQKFKDESEDYVAHMWRRVALSSKVTAEQLSSYQNAIEALTSVESNWLKIDYLLEFSQWLYVNQFPIEDSIDQVEWAVDILLNMKFESPLKKDALSDSTNNLSKDNKKEKKKGRMSAKKPKTPELEKPVIETVDIKSEKAESEKEIKAEDYVPVVKQADIGHTLFFVLDICVGPVFLCILGVLPRDMNLTIEDLTDIQQLDYLFRAHVILAQIHGRGSPCYKDTLLTAYSYLQRIWQVSITASGPTLKELAKVPV-----TETAMSGKNSAKSKGKKDKEKEVVKEKPKRKGPLDVLPVSTEEWANYDVPDEVLEAFKHEAMNKHGVNTHTLTKPLLTSHY--LEELTEELRALGYNHLSLPVLAFQDLMARG--IFNNKAMSNLYHIKSLEVCYELDIKTGCLFHEKAVGNMEISEEEQARSRDEIAIWKEKQVQVAREELRVKESLVRLATEGKIEKRSLTSKTMNS-PNKEEKLETVQHLGKVLGAVS-MREIWTLTAEVLIRQGYNQHAREYLHEANLAAQAFGDKKLESGILYILGQLAFNEAQYGQCFNLCRSAQNLQCGDEMFWFKSTMLIIEATLK---DYENKNNKRVARKIVLHAMKEF--NQIYEER--------PNRLSSICFILSSFEAKLATIQSTILMEGGRKSTVPKVMKGLLSACEKFENSIEKLLGLGYRREALPFIKQHADVLKHIAREAIEPEIKHTYLLQSLAVLKEGVAITEDLFADSLSLATLQDSRSMNSPLQRELVEIQIKCGELLIDILKLQSQEVRVQQLNDERKGSVLLLVEDYIRETPKYTHMEKDWVEIKQSVGEEAMLLLINSHSLAGNIIPLRTQSLLSIGKCLHLLGQSLHPNPPSQWLNKHME----MLKGSSELGENQDVESEKLI----------EETKQTIKYSYQIKQKQKLYESSRSYLLQACECLLQALNLSLNHHLFKLASSASLEMVELMGQFDPMAASQFLALHQSCETSVNLGSLLQRAQLDPVTSKLSALLHQRNNILDSDI--QTNLSSSTIMSSVNSALEKDWQAWKRLDVVSNHLELLKEFPP-NFNFIVLQHSPDRSFLYGAILDKPKHSSTGAGGKGGKHHSSSVPSRAKIFGVETSPKILEELVERFRFHKQSVQQLQLKQEYQRTQAALRNKMLKNIDNSIK-----QEAKVQTAEDQLEEQRLAEEFQQLVIAMETYLKPVINPLHNAL---RSTSSPTGSMANLAKEPKDHVVSP--ECIILLADPVLLEMPLEALECLQSESVSSLSRDFSLQMFSHRFHQEALAGEDPNTDKHKKKAKDAGQPMS--RIPGAREAKQKQAKLVPLDRPIQPWQFPVDTMNFRCILYHIDKALVNMRVLGGACMSCLFDHCYDDCAETEENKPVTLFNHLLT---EYEQQFTPRWLGLTGDDHSPSVGEWEVYLKESSSFIFYGMERLLSYIPPSKLAALNIPDCVMLFCLDMTQTNKSFLRQSKIDILKSHNDLSLEKPVESAMLSSLSGIRCLLANQWPCTLAENSHKLTLAFKDLLEKGKTTGQSVRLMMSPQKRANETRAEEEAKELEAASSPAGKGKDADKHDDKHPKGTTGTPDVGLESEKVPE------VPPESESQIKSSWFNLVCYGLPNVVIT 2893 HSP 2 Score: 163.31 bits (412), Expect = 6.215e-36 Identity = 76/224 (33.93%), Postives = 135/224 (60.27%), Query Frame = 3
Query: 105 INECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKI---MKDKDD 767
I+E +D+ V+ E + + + CL+++F P NQ+L RAYL QAQLI P + L+K + +I+KAI +K +R+H+++YN+++IY++ R +L+P + QYL SL I K L++++D + ++R+QLM+ LI+CH+DAG R + I ATF+ ++ P + K + +++N + K KN P + + ++CK+K+ MK++ D
Sbjct: 41 IDETEAFNSDLYVLCAETAYQYGLTDMTRECLKMFFMKPPPANQFLCRAYLCQAQLIAPHDANNWAQLEKAVVYIVKAITIAKTNQRYHFIVYNASVIYWQFCRPFLKPGYRQYLATSLHQIVKALDDIDDQDFEWRAQLMIGLIECHLDAGRRSDASHIATAAATFIKQNVPAMFKQVFGLMVHNQLVETSKLHKDIKNSPELSVYYKICKIKLSLEMKEQKD 264
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Match: A0A2T7P8L8 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_09022 PE=4 SV=1) HSP 1 Score: 682.174 bits (1759), Expect = 0.000e+0 Identity = 615/2469 (24.91%), Postives = 1132/2469 (45.85%), Query Frame = 2
Query: 932 LLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHG------KFTKTFS-----WENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNS---------TESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKV-SPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCL-HKTGQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKK--------RLKLFLEPHTXXXXXXXXXXXXXKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNTEATK----EKSLSHPNSSV-------KNRSRNNFRKP-----GSGLKPNVKNENIQETPEMXXXXXXXXX----XXXXXXPTWFFLQEFRPRTLE---------------DICDIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINS-------PFVIPEYCAHVEIIKANLEEYKKQNKSQVLPMNFETQSTL----PLKLSHLNRKLTKFGYI--HETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAIGSCHFTFKQ-WSEIIDLLIE-IGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATL----------DLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFK-SLLIKKLIVIEKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEV-IPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDT-------EKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANL---SKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVE-NKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFPPGNERNSPSNQKANKETISQPGKDSIIILTDEILSVASLESIPKLKSSISRDFSLQVLHHRL--KGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINK---KSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLRE-QQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMIN 8011
LL+ELA + L + C+ +++ ++ +Q +F+ L++ E + + RL+ I++C+ ++N IR + +I+ GC + WN+CLP+LQP+L IH L L+A++LE I L KL Q+H E+ K + EQI A+++L+KA+ LD Y E +E + +L+++ +LY +P P D+A ++EQ + + ++ R +L + G LAPD FL++LD END +G F+K S + + V++ +L R G +D+ + RLW DL K +RK +WD+CRVA+ F +LYDDGRWK + S K R G D + + +E ++ L+ N + ++ L+ L E FI EA + LR+EG QL P P+ K PKG D +W YC + +LS + FLR L VDL PW NAA Y+WNY+ HILT H ++ + D L+++ + ++ N LA +IQ ++P ++P K +KD++ SK + Q E+ KKA+++C+Y +++TS + I + +++ WV KQ+ QQ +G+ GTDD GQ+ + RA+ + +M+ + N +F D+ S+ D Q ++ C W ++ + L +L +L + S+ N +++ ++ A+++ S +H+ K + + V ++ L++ G L G S+ ++ + +AR+ +Y LV + ++W L+ + + + I+ I++C++ T +KK ++ L EP + + E+K P + + T+DN DLT+R M+ +FQ + Q + +LK++D+A+AD+PRT H+L +F+HR++TK +L ++++D QKF+ + E+ VA +WR++A+ SN QL +Y+ +IESL + SN KVD ++E SQWL+++ +P++ +++AI+ILLN + + +K + PN++ K R +K G +++ ++ E MT+ E T +F P+ E D+ +++ L V A+L G G D L+ A G ++R+WQV + +K + + E ++P K K A +K + + SE QS K +R+GP D +P EEW+V+ D+ +EA + MK +GL+ + P M D L L GY L P+L+ D +SRS + LN IH R L+ L ++ + HE + + F E A + KA + + + K + E+ S SHL + L G + E W +A +L+ +GY Q+A E + A ++ +D + L KL + +F +A + A H + W E + LL+E + D N++ R L++ H N +K++N++ N +N+ + + + A++ D+ ++V + E +E++ L K+ + ++ E L + + + ++ L V+++ + + +F D + S ES + P+ R+L + L+E+ ++ V++ L + K + ++V+EY + + K W + ++ + L + H++ I ++ + L G V+++ A P+ W + +IE + ++ + + K + + L ++D KN Q + M+K Q ++ Y A E + Q ++++IA + L+ FGQFD L+ Q L L+Q+ +T+ ++ +L K R + +LAA + Q +IR + +NL + ++ SV + L AWK + N L++LK+ F+I +HS ++ FLY A ++ + S P G R KI + T P ++ + +R K ++ L+ E +TQ +++ L + ++ Q+ +E I +EE++ ++ +++ YL P+ + + +++ +N+ I P D ++ L E LS + I S++RDFSLQ+ +HR+ + E+ VD KK K + P L+ + N+ K +K + R + +A++ N ++++VDP ++ S ++ G F++ +L + +Q+T +W+G+ G+D P+ E ELY+KE+++ ++ E ++ SP L +N
Sbjct: 309 LLMELAHLCLDLDLGKVASVCVEEMKTCPVKDQSFCIQ-MEFLECLLMVKSLGENQESYQKSVVDLRLQAIKRCEEAIMNAIRHGDPSVIQAGCVAEWNLCLPLLQPNLRHYIHKTLSLIAEALENIQSLLIKLRCQLHTELAKCEESKEQIQVAMEHLKKALALDDGNVYQERLEVMLRRLQLRSQLYRQPERPEDQAAMIIEQAQKADSGSVL---MKRSMLVKAGEALAPDAFLLVLD-------SENDSRDNGTIKSPLTFSKKLSAKARFFNHYVSKAEGHLKRLGKENDRERARLWADLAKTARKQGVWDVCRVAARFCLLYDDGRWKGSLPRPM---SSKQERTKG---------------------DTKEKTEDETDKKAGSRPLTPNKPVE-LYDRDLVRMLAEVNFINGEAIVLLLRSEGVQLNDKPIFPESHSKLPKGKTVAKPENDPDWLEYCNWIRELSEASTSSFLRGLELGVDLMEPWLVCNAAAYIWNYNNHILTQMRHREILLPLTAVLDGLKRVGHAGETSMLVNICNTLAFALIQPWIP----------EAP--------KEEKDQDDLNSKNKLKVSVSQGKIKGSAVSLVPEATVDIKKAVEVCEYAINMTSR---------------------------EHIVPIAARKLILQTWVMSKQMAQQQIGKTFGTDDESSTEGQKEMTRAIVATEMLSLNSNGIMEFKDVPSIADVAQMVENCQWTDSLIKLQMLTRLTSLAYH---SHQHNLVMRCSKAALELASINSHM-----EKKGDIHQYMVKQEM-LAYASILQGQSLVDNMGGKHSLRRDAMEAFLGGASFARNAGNYDLVMTAARHYWNACIDLVSQPMERQLLKEPIKTILDCITATWNKKSKIEERVEQVALTEEPKI----DDINKKDEKKTP-----STETSISTIDNQS-----------------DDLTLRAAMYGVLFQSYADQGELEMSLKAMDQAVADMPRTKHRLLIFKHRVITKAKLGQNVQMDFQKFQGESEDYVAHMWRKVALCSNDLREQLSSYQNAIESLISPSNGFQKVDYLLEFSQWLYYNHFPLQDVLDQVEWAIDILLNMQDEEAMQLKKEGTKPNATTLDSKKLSKAAKRTEKKKSKELPLGKASVTSLQKISVTEGSGMTSTGREDKALDGRLEVSDQETEISAGDFVPQAKEVVIGVLPSNPGLKISDLTNVQQLDALVRSHILLAELTGQDGTCYADILLQAQGYLVRLWQVLIKEAGPVMKIVQQGHPE-QAPPKGKGSAKEKKSTKEQKSEVTQSSVK-ERRRGPLDVVPELIEEWAVYNAPDELIEAFQQE-SMKKSGLNVDTFPKPMLTMHYLDILTRQLRVLGYNHLALPVLAFQDLLSRS--MLINHSLNILIHARALEVCLELDLKAGCEFHELSMGNISISEKEQFQSREEIALWKEKKAQVAREEMRVKDSLARTTAESSSRAKDGRKTTASHLGKVL---GAVCQREVWADLAEVLIRLGYLQKAREYLNEANIAAEAFSDTTLQGHIMYLFGKLAFEEAQFGQATNFCKAAQEIHQGDEMFWFETMLLLVESVICDYTNKNR-----RHLALKLLAHAVNEF--LKITNER---PNRYATLNFIMTTFEAKAASVQMQIILDHHKDINSPVAMKSVLTVCEKLDNCVEKLIRLGHRRKA----VTLTTEHASYLIMLARAATEVQVEHGYYLEALSVLKEAASLAEQVFTDALTLTSLQESQTLSFPVQRELANVYACQGELLVEVAALQVKEMRSIQLAEQRKGSIIRLVEEYTHQSPLLSPTQKGWLSKRLTVFEEAMTVLINAHSLAGNITELKIKALAGLGKVLRILAEHKDPDPPPLWL--VWEIEQLYQNADTEETEQEATEAGKINDKQDHVLASDDDNTTETRRFSKNSQLILSMTK---QHEASKLYYVYATECLFQGLSLAIAKKHKALAGQIALDLVKCFGQFDPNLASQFLALYQSCQTSLKLEELLYKAQRDPTSSRLAALLHQHQTLIRNNI--STNLGKSNMMTSVLKLLEQDCQAWKQLEILPNHLDILKEFPQ-NFNFVILQHSPDRDFLYGAVMEKPKISGGGGGKSNPRVSTGPGGSRAKIFGVDTDPRVLQSIIEKFRQHKQNVQTLLLKQEYRRTQAIQRQRMLENTDDDSKLSQKGTVE-------ILDESQHLEEDFQNLVNDMENYLRPVIAAVDAALSLTSSKDA-----SNECFILLPDAD-LLELPLEALSCLQADGI----DSLTRDFSLQMFYHRMFSGAVTENAGVD--------KKKKQEVPQVLSRIQGANEPKGKQSKSNLVKRSVQPWQVAVENNNIKYIVDPYREGSETDERKPGDQFRH-----ILEDYEQLTHKWLGIYGEDHTPSVGEWELYIKESSTFLFYGFERLVAYLSPAKLSALN 2601 HSP 2 Score: 149.058 bits (375), Expect = 1.612e-31 Identity = 77/229 (33.62%), Postives = 134/229 (58.52%), Query Frame = 3
Query: 111 ECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDD-----GSVIN 782
E F Q D+ V E ++ +K I +CL+++F NQ+L RAYL QAQL+ P+ ++ LDK + +ILKAI+ +K+ R+H+L+YN+++IY+ R +L+PNF Q+L +SL + + L E +D + ++R++L + LI+C +DAG + + I A F+ ++ P+L K + ++ + + L K K P + + R+C+LK D + G +IN
Sbjct: 31 ELFGQ--DLFVQCAEIAFEHGLKDITRDCLKMFFLKQQPTNQFLIRAYLCQAQLLAPQDANKPEELDKAVVYILKAISLAKQTDRYHFLVYNASVIYWHFCRPFLKPNFRQFLAQSLHQVVQALGETDDQDFEWRAKLTISLIECLLDAGQKADASSISMVGAAFIKQNVPHLYKQIFGLMVRHQLVELTKLNKEAKTSPELIIYYRICRLKCSLDLQEVRDYQGEIIN 257
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Match: A0A267H1S6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026945g4 PE=4 SV=1) HSP 1 Score: 672.929 bits (1735), Expect = 0.000e+0 Identity = 601/2415 (24.89%), Postives = 1078/2415 (44.64%), Query Frame = 2
Query: 791 KILSSLTGVDEVSISSSSETLNTKPDQKSTIV--IPINSKKLT--NEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGE------NDKDVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHF-QIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSM----------KQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAG-QNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDA-EIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQN-TSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRL-KLFLEPHTXXXXXXXXXXXXXKLP------------HILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNT---EATKEKSLSHPNSSVKNRSRNNFRKPGSG------------------------------LKPNVKNENIQETPEMXXXXXXXXXXXXXXXPTWFFLQEFRPRTLEDICDIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGL--HEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIPEYCAHVEIIKAN---LEEYKKQNKSQVLPMNFETQSTL----------PLKLS---------------------HLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYI---DAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNS----DNLIGKMN--YGLL----LLDRRIATL-DLGRSRNWENVS-EMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKE-WRIVTNGICDKICSTLSDVHTICS---QIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKM----------------SKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPA--------AIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFPPGNERNSPSNQKANKETISQPGKDSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRLKG 7576
KI L D+V++ + + K Q ++ I++K+L+ + ++ LLLELA++++ + FD QC++ ++ E +F+N ++ + E ++ R + IE+C LLN R I+ GCA+ +N+ LP+LQP+L + L LVA SLE I L ++L QVHIE+ + A LEQ+ A+++L+KA D EY E I +L ++ +LY +P P D A Q+LEQ + + + + + R LLAQ+G LAPD F ++ + S G + +H K + + +I+ +L R G T+D+ + RLW DL + +R ++WD+CRVA F +LYDDGRWKN Q K E S+K + + + ++ + D DS ++KR +S +++ + + EK L+ L E FI AE+ + +R++G QL P PKD +K P+G ++ D +W +YC+ + +LS ++CF+R+A L ++L PW +AA Y+WNY H+L + +L + D LR+ ++ +++ N +G+I++++P P+ + ++ K Q A Q S E V K+A+++ Y +D+T+ P I + + L+++W+Q KQ+ QQ VG+ LG ++ + R + RA +V+M+W+ +N +F + V + Q + C W + V L L +KLA I ++ +N + +K ++KA+++ G ++ + + TV + LS G L + Q +V Q + + AC +AR Y L++ + +W + L+ + + + ++ ++ +S TVD+KRL KLF P +E A E+ P +Q +Q ++ LF + S LE+DLT+R M+ +FQ + + + K++D+AIA +PRT H+L +F+HR+LT+ +L S+ +D+QKF+N+ E++ A LWR +A+ S+ +SQL AYK +IE L ++ LKVDL++E SQWL+ + +P+ELC+ +D+AI+ILL E KE ++ K R + + P S L + E QE + NP+ + F + + R L + C A+L + R L A CV+R++QVA+ + A +E K+KVE + + + + + PKRKG D++P + E+W+ +++ D+ LE + M + GL H I+ P I + C L E L G L+ P+L++ D ++R + ++ N +HL + L +N + HE F E V+ + N L ++ ++ ++QV + TL P +L+ HL +L + +H W+++A +L E Q A ++ + + + D + +L + + +F +A + A+ + + W LLIE + +S+ + LR E +L Q+ N D L+G++ +G L LDR L GR+ W V+ + L +L + + F+ L ME + +++ + +L+ + D P + P R AKL L+DCL+++ I V++ + AK T+ V+E+V+E ES+ W D L D H++ + +P A L G ++L A L P+ QW L+DI DP + ++ + E++D+ + + +A + ++R L QA E++ Q+ +++ + D A++ L++ GQFD ++ Q L LHQ+ TA Q+R L LR +++ + AA + Q D + +++ N + + + ++ L T AW+ + V ++ K L GFL +HS + ++LY ++ + +KP + R+ + R TS ++E L + R FKS++ LV E + ++ + I + +R+ + L + ++ ++ ++ ++ + EY+GP + P + + S + ++ QP D +++L D L+ LE++ +L S +SRDFSLQ++ RL G
Sbjct: 270 KIYRQLLAEDDVTVQAPKGKKDAKKSQAASKADAPAISTKRLSQHSPEKPKLLLELARLAVDQGQFDLATQCLSAIKACPPLPDQHFYTEVEFLNCYFIVKKLGPNEESYAKPIVQLRQQAIERCHEALLNANRQGRPDTIQAGCATQFNLILPLLQPNLRNLVRKPLSLVAQSLEAIDSLQFRLRCQVHIELARIEADLEQVQPALEHLRKAASFDDFSEYREGIAAMQKRLLLRSELYREPDSPEDRACQMLEQAEAA-SGKAVGVRMRRALLAQVGLALAPDAFGAAMEAENSGAGGGGQPKLGDPTQLHLYAAKASQYRQSIDKISGHLKRLGATNDRVRARLWADLARTARSQEVWDVCRVACRFCLLYDDGRWKNPVPQAKPEGDSKKKKKQQHQQTSPDPTQPQQPQSGSGNASDDHDSDAEKSKRGSVDSRGGSDAFLDAASAAPATRPNLYEKELVRTLAEVGFICAESHVHLVRSQGVQLYERPLPPKDTRKLPRGQVRKDPEADPDWNAYCDWVTELSAFCLSCFVRSAQLGLELNEPWLVTSAAAYVWNYGQHLLAMGRQNLLAQHLLPLVDCLRRGGHDGRTELLVNVCTAAGYGLIREWLP-PLPKRQPAAGEGEDGADGAAAPAAKKKPGSGKAKPQAAAPVQISPEGVPDCKRAVEVTAYALDVTNGNRP------------------------EDAIAVGMRYPLLQVWLQAKQMCQQAVGKGLGAEEGLDSYDPPRQMTRACAAVEMLWLGRNGVMEFKEAPGVGEAAQMVTDCQWTDNLVALQLWVKLAFIALEMQENNLV---LKCSDKALQLDGHLDDGQQQQQPQIQQHRQTV-GREHLSLAASLKGQALMRLMQGKMAVRRQAMSAFLLACRHARQARSYQLIAAAARLYWNAAASLVGQPIERQLLLEPLQTMLEAMSATVDRKRLKKLFERPEGQEGSAAAAAPEEDDSPTDPEERRLEQKIQFVQSAPEQTVL------GLFPPAVSPPPSAKQLEEDLTLRANMYGVLFQAYADKGDLDKGSKAMDDAIASMPRTKHRLLIFKHRILTRAKLGQSVAMDIQKFRNEPEDKQAELWRIVALSSHGMKSQLEAYKNAIECLTLPEHDWLKVDLLVEFSQWLYTNGFPLELCTAQVDWAIDILLGMAFEEPVKE------DTDKKARGKARQKGPPSAASTVASQQSKKAAAAKSAATAGSAKDAAKTLPHQSEAEEKQEKDQQQMILPAEKRIVIGANPSAKPVASF-----SALFNARQLDNLLRCFCLLARLTTPASPAHRHALHAAHACVLRLFQVALPAGLTATREAAKTKVEEKPGGGGGKGGKGGKG----GGKEKEPQKEKPKRKGGLDFVPQTTEDWATYDVPDELLEVFRRQDLMPAAGLGRHNIAKPTITLYY--CRLLTEKLRNSGLSHLSLPVLALQDCLTR--DLCKSRPANCLVHLTAMDVCLELNLHKGVEFHE------FCAGELAPGVDELTENRKMLVQW-RERRAQVRREEARARETLAELARELGRDPAELTAAAGAVHGGGGDAEDDSLAGGHLGEQLGEL-QLHTQWLEMARLLTEQAQYQTARLLLREVQLTASEIGDRRCLAGALQELGRLALREKQFGQAAQCVRQAQAMMQGWASEEDWFLSCQLLIEATAGDYTKKDALSRAKTEALRSIAVFE------ELGRQRSNKASLIDFLVGRLELLFGRLQIAWALDRLSQRLCSRGRAHRWLEVARRFLHCGLERLTNGRDFRHALECFQQLGTCQMEMSRL------ASDEEGKRQMLVDAASCFRAATRYAEAFVADAQTSGPPGDLRKVCPPAHRLAAAAKLSLADCLLQLLEIQVDEERTQAELQLAKSADTKAVEEFVQETPRLTESQSAWMSKVRLAFDLAVGQLQDAHSLSAPGGPLP-TRAHSLALIGRALRLLASRLDPDSPAQWR--LLDI-DPSAWPAGDSGDTAAAAAAGTDAEVAEEAGEDKDSSATAVKTPPLVAAAAREREAKAARRTRQAWTAFQRSRDRLAQATEVLQQAQTVALHKDALDVASQASQELLECIGQFDLSIAWQQLTLHQSCETAKQLRRTLRLALRDSNLSETAALINQLDNL--EAEAAFCNGLQSRVAAAARDTLRRTSLAWRQSCVDHAHQDIGKSLPPFL-GFLALQHSPDCRYLYAGAMERQRA----AKPGKAAQSGAGAAGHPLQSRSFVLRRDTSKERLERLLDEVRDFKSEVGTLVLKHEYQRSQAAKQKRMLEAITAGVDGERSGSANQ-LTEEHKADLELLQTWFARLVADTAEYIGPALHRFERLNRPSSA-TTVSTSRREQQQQQQP-DDCLVLLIDPNLAELPLEALQQLHFDSLSGMSRDFSLQLVARRLFG 2595 HSP 2 Score: 197.208 bits (500), Expect = 0.000e+0 Identity = 87/231 (37.66%), Postives = 149/231 (64.50%), Query Frame = 3
Query: 87 LISKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDDGSVI 779
+++ + NE D+ V+L E +KN + I+ CLR+Y SLD NQ+ ARA+L QA+L++P + AL+KPI ++LK I +K ++H+L++N++++Y E+VR +L+P+F ++LC+SLS + K LE ++D + ++R++LM +L++C+IDAG RKE + + + A F+ K+ P KDLL + NS+G K W+ + + + RLCKLK+ + D V+
Sbjct: 33 ILADKKPNESLPIGFDIFVLLAEEAVKNGLDAISKECLRIYLSLDAPNNQFRARAFLAQAKLLQPTSSEHPEALEKPIAYVLKTIELCRKIPKYHFLVFNASVVYSELVRPFLKPHFRRFLCQSLSQVVKALEAIDDKDYEWRAELMKILLECYIDAGLRKESLLLAEQAANFVTKNVPGHFKDLLELISVNSLGEHGKLQWWIRGSAELAIYYRLCKLKVAIKERDAEVV 263 HSP 3 Score: 122.479 bits (306), Expect = 1.870e-23 Identity = 70/219 (31.96%), Postives = 112/219 (51.14%), Query Frame = 2
Query: 7652 RLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMET-----RKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNP 8293
R+ RD +KK +KIIPL R +P G +A+DT+ +R +VDP D + + F+ M R QQ TPRW+G+ GDD P+ E E+YL+E I+ E F++ P + + D + + FD +T R++K +V++S LR + A L++L GV + +QWA + N + TF + L+ G VG+ + + P
Sbjct: 2647 RIPGLRDASKKQSKIIPLVRPVPPGHLAVDTSHIRHVVDPYLDCAETEQWKPLDQFRAMMERPE-RAQQFTPRWLGVTGDDHAPSVGEYEVYLREGTGFIFYGMERFLAHVPPHAVASLGLTDCRLVALFDMAQTSQSFLRQSKIDVVKRSD-----QLRLEKPVETALLMTLAGVPALCCHQWASTLKENATRLGTFLADVLEGGKTVGQAVNFVYEP 2859
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Match: A0A1W5BDM0 (cilia- and flagella-associated protein 46 OS=Ciona intestinalis OX=7719 GN=LOC100175618 PE=4 SV=1) HSP 1 Score: 642.114 bits (1655), Expect = 0.000e+0 Identity = 610/2513 (24.27%), Postives = 1130/2513 (44.97%), Query Frame = 2
Query: 1100 EKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDK----DVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKF--PDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITV----KGSVSNSINTLVKITEKAV--KVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKT-GQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLFLEPHTXXXXXXXXXXXXXKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNT--------EATKEKSLSHPNSSVKNRSRNNFRKPGSGLKPNVKNENIQETPEMXXXXXXXXXXXXXXXPTWFFLQE------FRPRTL----EDICDIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIPEYCAHVEIIKANLE------EYKKQNKSQVLPMNFET--------------QSTLPLKLSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYI---DAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGD----ISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQIIFDDIYRVCS---PLESSA-FNLPIDRKLCRAKLMLSDCLIEMFSITV-EDTAEKVLQHRAKDHLTQIVDEYV--EEVIPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPD---INAQCLLKYGHVMKLRAMELYP-NKRNQWSRPLMDIEDPLETQKSKLVNLQDSSN--NKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRY---YLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGF--LIFEHSLNQQFLYCAFID----TKHKTANLSKPNNKPAAI---GDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQT---EIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGN-FFPPGNERNSPSNQK-ANKETISQPGKDSIIILTDEILSVASLESIPKLKS----SISRDFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPH-----RLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPS 8347
E + ++++ RL I++ + L N +RS N+ +++ CA+ WN+CLP+LQ +L + L VA +LE I L L +VH E+ K A+EQI A+K++ KA+ LD Y E ++ +H+L++ +LY P P D A ++EQ R + +E + R LL + G LAPD F +LD + K K +V + W V + +L R G+ +D+ + RLWVDLVK +R+ +WD+CRVA+ F +LYDD RWK + + K E S ++G+++ S+ A + DS+ K D S + + LL L E ++I EA I LR EG +L P+ P D ++ PKG ++ EW Y + + L+ Q + FLRA L ++L+ W + A YLWNYS H+L S + L FQ + ++Q+ + ++ V+A G+I+ ++P L ++ + +A+T + K +S + G T + ++ K+AL++C+Y + +T+ + I + QL+ WVQ KQ+LQQ + GT++ +I QR + +++ ++M+ + +N+ +F P++ V D +D C W + V L + +LAQ++ V N + + +KAV K + E + KR TV ++ L++ G L T N S Q + V + + ++ LV +W + LI + + +E+++ +S+ + EP + K ++++ D + + + +++ + S D T+R M+ FQ F + +W E L +D A+ +PRT H+L LF+HR++ K +L ++++D+ KF+++ E+ V+ +W +A+ S QL A++K+IE+L + K + ++EL+ W++ +++ I+ +++A +IL+N + ++ VK SR + KP++K + ++TP + E E P L ED+ R L + A + G R +A V+R+W+++++S V +K + K + T+ + K + + PV+ + KRKGP D LPTS EW+ +E D+ ++A G+++ +I G + L E L + L+ P+L + I+R + + Q I+++ + ++ +A E +I++ F+ + I K LE E KQ + ++ N + P ++ RK+ + E W + A +L+++GY Q ++ A ++ ND + + A L ++G +A+ + AIG W E I LLIE T + D ++K+ R L D KLS + ++ IG + L IA R + + +L KL + E + + L I ME + L K N+++ + I + +++ Q D++ R+C+ PLE+ +LPI R++ +L S L++ ++ ED K + AK L + V+ YV E ++ + W + S L+ +HT Q+P + A+ + G ++L + ++P + + QW D+E L Q + V ++ S+ + +E + + + + Q + ++AK+ + + L+QA E+ Q I +SI + D V A +++ Q D Q + L LHQ++ ++ M+ +++K++ S QLA+ + Q ++++ S L ++ K + + L+ F AWK ++ N L+LLKD+ S F +I +HS +++ LYC +D + T SK +K + R + +I P + ++ + F+ +L + E + R + ++ I LE++A M ++I E + +I+ ++++Y+ P+ + P E+ +NQ +N + +S+ + IIIL L LE++ L++ S++RDFSLQ+LH R DP +A + + K+ D P + A + + + KI+PLNR+LP +IDT+ ++VDPS + S + F+ + + Q TPRW GL G + P+ + E + ++ I+ E FI+ P + +N ++ Q D+ +T K+ + + L + A L+SL G NQW + N ++ TF L+ G+ VG+ +++I NP +++ +++ ++ +P+
Sbjct: 11 EAYSKSSVEVRLSAIKRMEQALENAVRSGNANVVQGACATQWNLCLPLLQRNLRKHVRRPLTAVAKALEDISSLQVTLRCRVHTELAKCEEAVEQIEAAVKHVNKALELDDSGFYHERLQTMLHRLQLSTQLYRTPDRPEDVAAIIIEQARKS--SESGSVRMKRSLLVKAGLALAPDAFQYVLDSENETKASGGMKGLVSEVQILGARARQWSKCVKKAPGHLKRLGDENDRERSRLWVDLVKTARQQHVWDVCRVAATFCLLYDDARWKVNPKEK-EPSEKEGSKDKRKSANTSKAPGTG------------DSASVAGK----SPDTDQGSTDSNLHDADLLRMLAEVHYINGEALIHLLRCEGLKLNESPDPPVDNRQRPKGHEYKKPEQEPEWAEYTDWIHHLNKQATSAFLRAIELGIELEETWIICSGAAYLWNYSNHLLVASRYTELAPMFQQVLFGMQQIGHSTEATLLVRICEVIAKGLIRPWIPKKSSLEGSVTQATTAATKDTSRKKTAGKSTGNTAPGTTAGLAVDPDGMDDVKRALEVCEYGLQVTNG------------------------SNQGDIISTSVRHQLLATWVQVKQMLQQQISPGFGTENEKIP-EQRDMTKSLVMLEMLSLNRNDMMEFTVPEISEVCDA---VDECSWNNSGVELEVWSRLAQLSRLQKNHSLVVRCSNNALALEQKAVNKKHTKEKQADIHKR---------TVALEM-LAYASCIRGESLMDTCAANPSARRQAMHSFVDSAKFGERAGNFSLVMMACRRWWNAALPLIPSPLERELLRQPLEVLL--ISILRTANNAAVVDEPE----------DSGGKEKGVVKVIDGEGDGSSNTKQSIGV-------SLGDPSDDRTLRSAMYGLQFQTFADKGEWEEGLVVMDRALHHMPRTKHRLLLFKHRVIVKAKLGKNVQMDIAKFRDESEDYVSHMWHRVALCSRETLEQLMAFQKAIEALHSDDTQWQKFEYLLELATWMYSNEFSIDDTVNIVEWAADILINMKFHVELPEQTQADRGKGKKGKMVKPASRAQPPPKTTESKPSLKQQ--KKTPASRQKKQDEPKKVEEKQEEQHLPVEREAIIGVEPNNLNVKFEDLHSTRQLDGLIRSHVMLATISGKGSKSHRTFCQLAYSFVMRLWKISLTSAGVVMKHLPKPETTAADDTRKGSGSKKAKATPA-QTAPVKERI---KRKGPPDALPTSTSEWATYEPPDEIIKAFLFDT--SDCGINKGTITCAGMTVFYLLKLSEYLRDCSLHNLSLPVLCLAAVITRG--ILQSDSWTQLINIQQITVCYELHLINAAEQWENWIDNKFLTEQ---EQTISKQELEIKQISKELAKQEEERIRKANGDALVQDTKTSSAKSTSSKREPGPITLTGRKVGGVN-LSEIWAQKAKVLLKLGYYQHCRSLLNEALTVANYNNDTVLLVDIYHGLADLATHEGNNGQAIQLLRKAQAIGGDELF---WQENILLLIEAITLNQDPSPDDVVKVTKVIRRSLECLD---------KLSATRPTKNSTIGYLKSIL------IARSSFSR---LASCTSQPAVYLRKLVDAERTLQDLGFHRELASIHMEHADALLKLSKQNKESQH--------VYILQAIKLLQTSVDNVTRLCNDVVPLETDKEVSLPIHREMMNLQLHFSSLLLDAAEVSANEDRLSKAAE-AAKGALERFVENYVGTAESELNETAMLWNAQRTTFVHRAMSVLNTMHT---QLPTQHVLRAKWMYLIGRSLRLCSEGVHPDDPKYQW-----DVEALLPEQMLRDVGIKSSTGFMSTSMEEVNEDPTQQQHDVTSRQMKLFSNEAKTLDSTSEFSYKLLSQATEVFAQCINMSIQLQAIDIVSLAALEMVECCAQHDPQTATLYLALHQSAESSKAMKELVMKSVSDPSTSQLASLLHQDSWLMKGYI--SSGLDNCTVKKDIMKLLSDKFTAWKRLQLASNHLDLLKDMP---SNFNIIIMQHSPDKRTLYCGILDKPKSSTSTTDGKSKGKDKQTSSQTDTSRAAVYKIHVDPEKYNEMMDQHSDFQQRLMSFLLQSEYQRSQLAMRQKMLQQLSKSATVISISETALEEQA------MEEMKIREEFGDILNDMEKYVEPLSSHIKTKLLQPIIEQ---ANQHLSNGQLLSELTPEMIIILAGPTLMQLPLEALKCLQTPHVDSVTRDFSLQMLHRRWF----VDPEEAAAAAEKKRNDLKTAPKTPKGGKDAGKKGVATPAAKIVPLNRELPPDCASIDTHSFHYIVDPSNECSETEQYRPAQVFQQLLQ---MYGQTFTPRWNGLFGTEVFPSVGQWEEMINNCSAFIFYGMERFIAQLPPAKVAALNLDECQLALLIDKSQTNKSFRHQSKVDVDKTNTLLSIEKPLQTAMLLSLSGANCVCLNQWHTTSESNAAKLHTFMECVLEQGLTVGQAIRKITNPTLKKPEPVVEEVAPITPA 2374
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Match: A0A267GM73 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026945g3 PE=4 SV=1) HSP 1 Score: 526.168 bits (1354), Expect = 0.000e+0 Identity = 339/1169 (29.00%), Postives = 589/1169 (50.38%), Query Frame = 2
Query: 791 KILSSLTGVDEVSISSSSETLNTKPDQKSTIV--IPINSKKLT--NEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGE------NDKDVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHF-QIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSM----------KQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAG-QNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDA-EIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQN-TSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRL-KLFLEPHTXXXXXXXXXXXXXKLP------------HILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLN 4186
KI L D+V++ + + K Q ++ I++K+L+ + ++ LLLELA++++ + FD QC++ ++ E +F+N ++ + E ++ R + IE+C LLN R I+ GCA+ +N+ LP+LQP+L + L LVA SLE I L ++L QVHIE+ + A LEQ+ A+++L+KA D EY E I +L ++ +LY +P D A Q+LEQ + + + + + R LLAQ+G LAPD F ++ + S G + +H K + + +I+ +L R G T+D+ + RLW DL + +R ++WD+CRVA F +LYDDGRWKN Q K E S+K + + + ++ + D DS ++KR +S +++ + + EK L+ L E FI AE+ + +R++G QL P PKD +K P+G ++ D +W +YC+ + +LS ++CF+R+A L ++L PW +AA Y+WNY H+L + +L + D LR+ ++ +++ N +G+I++++P P+ + ++ K Q A Q S E V K+A+++ Y +D+T+ P I + + L+++W+Q KQ+ QQ VG+ LG ++ + R + RA T+V+M+W+ +N +F + V + Q + C W + V L L +KLA I ++ +N + +K ++KA+ + G ++ + + TV + LS G L + Q +V Q + + AC +AR Y L++ + +W + L+ + + + ++ ++ +S TVD+KRL KLF P +E A E+ P +Q +Q ++ LF + S LE+DLT+R M+ +FQ + + + K++D+AIA +PRT H+L +F+HR+LT+ +L S+ +D+QKF+N+ E++ A LWR +A+ S+ +SQL AYK +IE + ++ LKVDL++E SQWL+ + +P+ELC+ +D+AI+ILL
Sbjct: 270 KIYRQLLAEDDVTVQAPKGKKDAKKSQAASKADAPAISTKRLSQHSPEKPKLLLELARLAVDQGQFDLATQCLSAIKACPPLPDQHFYTEVEFLNCYFIVKKLGPNEESYAKPIVQLRQQAIERCHEALLNANRQGRPDTIQAGCATQFNLILPLLQPNLRNLVRKPLSLVAQSLEAIDSLQFRLRCQVHIELARIEADLEQVQPALEHLRKAASFDDFSEYREGIAAMQKRLLLRSELYREPDSAEDRACQMLEQAEAA-SGKAVGVRMRRALLAQVGLALAPDAFGAAMEAENSGAGGGGQLKLGDPTQLHLYAAKASQYRQSIDKISGHLKRLGATNDRVRARLWADLARTARSQEVWDVCRVACRFCLLYDDGRWKNPVPQAKPEGDSKKKKKQQHQQTSPDPTQPQQPQSGSGNASDDHDSDAEKSKRGSVDSRGGSDAFLDAASAAPATRPNLYEKELVRTLAEVGFICAESHVHLVRSQGVQLYERPLPPKDTRKLPRGQVRKDPEADPDWNAYCDWVTELSAFCLSCFVRSAQLGLELNEPWLVTSAAAYVWNYGQHLLAMGRQNLLAQHLLPLVDCLRRGGHDGRTELLVNVCTAAGYGLIREWLP-PLPKRQPAAGEGEDGADGAAAPAAKKKPGSGKAKPQAAAPVQISPEGVPDCKRAVEVTAYALDVTNGNRP------------------------EDAIAVGMRYPLLQVWLQAKQMCQQAVGKGLGAEEGLDSYDPPRQMTRACTAVEMLWLGRNGVMEFKEAPGVGEAAQMVTDCQWIDNLVALQLWVKLAFIALEMQENNLV---LKCSDKALHLDGHLDDGQQQQQPQIQQHRQTV-GREHLSLAASLKGQALMRLMQGKMAVRRQAMSAFLLACRHARQARSYQLIAAAARLYWNAAASLVGQPIERQLLLEPLQTMLEAMSATVDRKRLKKLFERPEGQEGSAAAAAPEEDDSPTDPEERRLEQKIQFVQSAPEQTVL------GLFPPAVSPPPSAKQLEEDLTLRANMYGVLFQAYADKGDLDKGSKAMDDAIASMPRTKHRLLIFKHRILTRAKLGQSVAMDIQKFRNEPEDKQAELWRIVALSSHGMKSQLEAYKNAIECITLPEHDWLKVDLLVEFSQWLYTNGFPLELCTAQVDWAIDILLG 1402 HSP 2 Score: 197.208 bits (500), Expect = 0.000e+0 Identity = 87/231 (37.66%), Postives = 149/231 (64.50%), Query Frame = 3
Query: 87 LISKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDDGSVI 779
+++ + NE D+ V+L E +KN + I+ CLR+Y SLD NQ+ ARA+L QA+L++P + AL+KPI ++LK I +K ++H+L++N++++Y E+VR +L+P+F ++LC+SLS + K LE ++D + ++R++LM +L++C+IDAG RKE + + + A F+ K+ P KDLL + NS+G K W+ + + + RLCKLK+ + D V+
Sbjct: 33 ILADKKPNESLPIGFDIFVLLAEEAVKNGLDAISKECLRIYLSLDAPNNQFRARAFLAQAKLLQPTSSEHPEALEKPIAYVLKTIELCRKIPKYHFLVFNASVVYSELVRPFLKPHFRRFLCQSLSQVVKALEAIDDKDYEWRAELMKILLECYIDAGLRKESLLLAEQAANFVTKNVPGHFKDLLELISVNSLGEHGKLQWWIRGSAELAIYYRLCKLKVAIKERDAEVV 263 HSP 3 Score: 183.341 bits (464), Expect = 5.934e-42 Identity = 250/1134 (22.05%), Postives = 473/1134 (41.71%), Query Frame = 2
Query: 4424 DIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGL--HEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIPEYCAHVEIIKAN---LEEYKKQNKSQVLPMNFETQSTL----------PLKLS---------------------HLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYI---DAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNS----DNLIGKMN--YGLL----LLDRRIATL-DLGRSRNWENVSE-MMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKE-WRIVTNGICDKICSTLSDVHTICSQ---IPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDP-------------LETQKSKLVNLQDSSNNKEIENNSY---GLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPAA--------IGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIFEKLGNFFPPGNERNSPSNQKANKETISQPGKDSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRLKG 7576
+ R L + C A+L + R L A CV+R++QVA+ + A +E K+KVE + + + + + PKRKG D++P + E+W+ +++ D+ LE + M + GL H I+ P I + C L E L G L+ P+L++ D ++R + ++ N +HL + L +N + HE F E V+ + N L +++ + ++QV + TL P +L+ HL +L + +H W+++A +L E Q A ++ + + + D + +L + + +F +A + A+ + + W LLIE + +S+ + LR E +L Q+ N D L+G++ +G L LDR L GR+ W V+ + L +L + + F+ L ME + +++ K LL+ + D P + F P R AK L+DCL+++ I V++ + + AK T+ V+E+V+E ES+ W D L D H++ S +P A+ L G ++L A L P+ QW L+DI DP + +++ +K+ S L+ E+ +++ + +A + ++R L QA E++ Q+ +++ + D A++ L++ GQFD ++ Q L LHQ+ TA Q+R L LR +++ + AA + Q D + +++ N + + + ++ L T AW+ + V ++ K L GFL +HS + ++LY ++ + +KP + + R+ + R TS ++E L + R FKS++ LV E + ++ + I + +R+ L + ++ ++ ++ ++ + EY+GP + P + ++++ ++ QP D +++L D ++ LE++ +L S +SRDFSLQ++ RL G
Sbjct: 1504 NARQLDNLLRCFCLLARLTTPASPAHRHALHAAHACVLRLFQVALPAGLTATREAAKTKVEEKPGGGGGKGGKGGKG----GGKEKEPQKEKPKRKGGLDFVPQTTEDWATYDVPDELLEVFRRQDLMPAAGLGRHNIAKPTITLYY--CRLLTEKLRNSGLSHLSLPVLALQDCLTR--DLCKSRPANCLVHLTAMDVCLELNLYKGVEFHE------FCAGEIAPGVDELTENRKMLVQWR-ERRAQVRREEARARETLAELARELGRDPAELTAAAGAVHGGGGDAEDDSLAGGHLGEQLGEL-QLHTQWLEMARLLTEQAQYQTARLLLREVQLTASEIGDRRCLAGALQELGRLALREKQFGQAAQCVRQAQAMMQGWASEEDWFLSCQLLIEATAGDYTKKDALSRAKTEALRSIAVFE------ELGRQRSNKASLIDFLVGRLELLFGRLQIAWALDRLSQRLCSRGRAHRWLEVARRFLHCGLERLTNGRDFRHALECFQQLGTCQMEMSRL------ASDEEGKKQLLVDAASCFRAATRYAEAFVADAQTSGPPGDLRKFCPPAHRLAAAAKQSLADCLLQLLEIQVDEERTQAERQLAKSADTKAVEEFVQETPRLTESQSAWMSKVRLAFDLAVGQLQDAHSLSSPGGPLP-TRARSLALIGRALRLLASRLDPDSPAQWR--LLDI-DPSAWPAGDSGDTAAAAAAGTDAEVAEEAGEDKDSSATSVKTPPLVAAAAREREAKAARRTRQAWTAFQRSRDRLAQATEVLQQAQTVALHKDALDVASQASQELLECIGQFDLSIAWQQLTLHQSCETAKQLRRTLRLALRDSNLSETAALINQLDNL--EAEAAFCNGLQSRVAAAARDTLRRTSLAWRQSCVDHAHQDIGKSLPPFL-GFLALQHSPDCRYLYAGAMERQRA----AKPGKAAQSAAGAAGHPLQSRSFVLRRDTSKERLERLLDEVRDFKSEVGTLVLKHEYQRSQAAKQKRMLEAITAGVDGERSG-SANHLTEEHKADLELLQTWFARLVADTAEYIGPALHRFERLNRPSSATTVSTSRREQQQ--QQP-DDCLVLLIDPNMAELPLEALQQLHFDSLSGMSRDFSLQLVARRLFG 2594 HSP 4 Score: 122.865 bits (307), Expect = 1.371e-23 Identity = 72/226 (31.86%), Postives = 114/226 (50.44%), Query Frame = 2
Query: 7652 RLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMET-----RKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPE 8314
R+ RD +KK +KIIPL R +P G +A+DT+ +R +VDP D + + F+ M R QQ TPRW+G+ GDD P+ E E+YL+E I+ E F++ P + + D + + FD +T R++K +V++S LR + A L++L GV + +QWA + N + TF + L+ G VG+ + + P PE
Sbjct: 2646 RIPGLRDASKKQSKIIPLVRPVPPGHLAVDTSHIRHVVDPYLDCAETEQWKPLDQFRAMMERPE-RAQQFTPRWLGVTGDDHAPSVGEYEVYLREGTGFIFYGMERFLAHVPPHAVASLGLTDCRLVALFDMAQTSQSFLRQSKIDVVKRSD-----QLRLEKPVETALLMTLAGVPALCCHQWASTLKENASRLGTFLADVLEGGKTVGQAVNFVYEPYKRANPE 2865
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Cavefish
Match: ENSAMXT00000040417.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000591.1:11312:17800:-1 gene:ENSAMXG00000041134.1 transcript:ENSAMXT00000040417.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 123.25 bits (308), Expect = 1.035e-30 Identity = 63/171 (36.84%), Postives = 98/171 (57.31%), Query Frame = 3
Query: 204 LYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYL---------LKDLLTTLINNSIGTLEKYL 689
+YF +P NQ+L RAY QAQL P +DK + + LKAI SK R+H+L++N++L+Y++ VR +LRP Q+L SLS + LE V +P+ +R++LML L++C +D+G KE T+ F+ H P + L DLL + + ++G L + L
Sbjct: 2 MYFETNPPSNQFLCRAYFCQAQLNSPHTVTTVEDMDKAVMYYLKAIEISKDYPRYHFLVFNASLLYFQTVRAFLRPGQRQHLVCSLSQVVSALEAVQEPDYAWRAELMLHLVECLVDSGKHKEAAAFSKVTSEFIEAHKPEMYPRIFSIQVLHDLLDS--SQTVGKLSRKL 170
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Cavefish
Match: ENSAMXT00000030730.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:12242508:12247413:1 gene:ENSAMXG00000038086.1 transcript:ENSAMXT00000030730.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 108.612 bits (270), Expect = 3.915e-26 Identity = 53/130 (40.77%), Postives = 79/130 (60.77%), Query Frame = 3
Query: 141 VVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLML 530
V+ E L+ K IA +CL +YF +P NQ+L AY QAQL P +DK + + LKAI SK R+H+L++N++L+Y++ VR LRP +Q+L SLS + LE V +P+ +R++LML
Sbjct: 43 VLCSEQALQLGCKEIAEHCLMMYFETNPPSNQFLCHAYFCQAQLNSPHTVTTVEDMDKAVMYYLKAIEISKDYPRYHFLVFNASLLYFQTVRASLRPGQWQHLVCSLSQVVSALEAVLEPDYAWRAELML 172
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Cavefish
Match: ENSAMXT00000039877.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002288.1:158806:177299:-1 gene:ENSAMXG00000029056.1 transcript:ENSAMXT00000039877.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.0922 bits (170), Expect = 3.350e-12 Identity = 60/246 (24.39%), Postives = 107/246 (43.50%), Query Frame = 2
Query: 7538 DFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRK---TKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVG 8266
DFSLQV + RL+ PV++ + + K + A + K+ K+ P+N LP S+ +DT+ +++VDP + + S + + + Q TP W G++G + + ELE L +S I+ F++ P L +N + Q FD ++ ++ VQ+S SK + + A L++L G S + NQW+ N ++ L ++G
Sbjct: 9 DFSLQVFYTRLQ---RQQPVESDNKKETKGG-------KPAKGKGDQSKAIKVAPVNCVLPPHSLPVDTHNFKYIVDPYNEGGDCEWSSTAEIMRKTLQ---MYSHQFTPLWEGVIGSESTCSPTELERLLVNCSSFIFYGMGRFLAHIQPSRLATLNLAECQMAILFDLVQNSTLPCQSQQEVQESKFCSKTHVPLETPIQCAYLLTLCGAHSVMLNQWSSSARTNAQNMEAIMENLLKKKTDIG 241
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Cavefish
Match: ENSAMXT00000038776.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002288.1:158806:177388:-1 gene:ENSAMXG00000029056.1 transcript:ENSAMXT00000038776.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.0922 bits (170), Expect = 3.350e-12 Identity = 60/246 (24.39%), Postives = 107/246 (43.50%), Query Frame = 2
Query: 7538 DFSLQVLHHRLKGMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRK---TKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVG 8266
DFSLQV + RL+ PV++ + + K + A + K+ K+ P+N LP S+ +DT+ +++VDP + + S + + + Q TP W G++G + + ELE L +S I+ F++ P L +N + Q FD ++ ++ VQ+S SK + + A L++L G S + NQW+ N ++ L ++G
Sbjct: 9 DFSLQVFYTRLQ---RQQPVESDNKKETKGG-------KPAKGKGDQSKAIKVAPVNCVLPPHSLPVDTHNFKYIVDPYNEGGDCEWSSTAEIMRKTLQ---MYSHQFTPLWEGVIGSESTCSPTELERLLVNCSSFIFYGMGRFLAHIQPSRLATLNLAECQMAILFDLVQNSTLPCQSQQEVQESKFCSKTHVPLETPIQCAYLLTLCGAHSVMLNQWSSSARTNAQNMEAIMENLLKKKTDIG 241
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002904.1 (pep scaffold:Pmarinus_7.0:GL476575:294585:302054:-1 gene:ENSPMAG00000002655.1 transcript:ENSPMAT00000002904.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.5362 bits (140), Expect = 9.706e-9 Identity = 24/80 (30.00%), Postives = 45/80 (56.25%), Query Frame = 2
Query: 3821 ALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLK 4060
A+ +++ A++ PR NH+L L+R R+ K RL ++EL+ +F+ E A W +A+ QL Y+ ++ +L+
Sbjct: 253 AMATVNHALSSTPRGNHRLPLYRRRVWLKTRLGQALELESARFREGGERACARAWLSLALAHPHPAQQLLCYRHALLALQ 332
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Nematostella
Match: EDO49124 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGW4]) HSP 1 Score: 172.94 bits (437), Expect = 4.825e-43 Identity = 104/344 (30.23%), Postives = 184/344 (53.49%), Query Frame = 2
Query: 923 RCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHGKFTK----TFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKG 1942
R LL++LA++ L+ + +C+ ++ + + L E +F+ EL++ + E + ++ R+K I+K K L+ IR +I+ GC ++WN+CLP+LQP+L + L L+A +LE I L L Q H+E+ K +Q+ A+ +LQKA+ LD +Y + +E + +L+++ LY P P D A ++EQ R++ + + ++ R L + G L+PD F+ ++D + K+ K K ++ V++ +L R G+ +D+ + R+W DL K +RK +WD+ RVA+ F +LYD+GRW I SS +G
Sbjct: 254 RVPLLVDLARLCLENDCHELAAECLDSLRGPIKDLGMSL--EVEFMKCELMVKSLGNKKENYSKNVVEVRIKAIQKHKQFLITAIRRGIPNIIQAGCVTMWNLCLPLLQPNLRHHVRKQLTLMAQALEDIDSLLVVLRCQCHMELAKCEEDEDQLEYALTHLQKALSLDDSGQYRDRLETSLTRLQLRATLYRTPDRPEDLAAMIIEQARASDDASTVRMK--RASLVKAGEALSPDAFIHVMDSENDTKDPSGAKGPPTPLGKLAGMATQYQKAVSKAAGHLKRLGDENDQERARIWADLAKTARKQHVWDVTRVAARFCLLYDNGRW------SIRGSSGRG 587 HSP 2 Score: 131.724 bits (330), Expect = 2.093e-30 Identity = 68/212 (32.08%), Postives = 124/212 (58.49%), Query Frame = 3
Query: 132 DMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDD 767
D+ V+ E K + +C++++F NQ+L RAY QAQL P + DK + F+LKAINF+KK R+++L+YN++++Y++ R +L+PN+ +YL KSL + K L++++D + +R+QLM+ LI+CH+DAG +++ + + T F + P L +L I + + K TK+ + F ++ +L+ + + +D
Sbjct: 1 DLFVLCAEAAYKLGKLDVMEDCIQMFFLKPVPSNQFLCRAYFCQAQLHAPSSSESPEQFDKSVEFLLKAINFAKKNPRYYFLVYNASVLYWQFCRPFLKPNYRKYLAKSLHQVVKALDDIDDKDYDWRAQLMIALIECHVDAGRKEDATLVSNATLQFTKSNVPSLYTTVLGLQIRYRLIDISKVSKETKSSNELYVFYKIQRLRTLIETED 212 HSP 3 Score: 126.716 bits (317), Expect = 7.559e-29 Identity = 92/340 (27.06%), Postives = 157/340 (46.18%), Query Frame = 2
Query: 2153 AEAFIQALRNEGAQLLTFPERPKD-LKKHPKGFIKDTSN--EDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSAS-TNLDKKAKKDRESPKSKTVGQTGAGQNST----ESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQN---EFYKFPDLLSVNDCL--QY-IDLCDWEEASVLLNL 3130
+Q LR + QL P P+D +K PK + N E EW +YC + LS + ++ F RAA + V+L PW N+A YLWNY+ H+LT + +V + + D ++ ++ ++ + G++Q + +P + +PS + K K R+S K G + A E N+ K+AL++ +Y +++TS P + + + LI+ WV CKQLL Q +G+NLG DD E Q P+ +++ +++M+ + N EF + P L V++ + QY + C +LLN+
Sbjct: 585 GRGALQFLRTQNVQLGDKPIPPEDTVKLRPKRYEDQQVNLEEQPEWVTYCNWISSLSQEAISSFQRAAEIGVELNEPWLICNSAVYLWNYTNHMLTQGRYRDIVQIYTPVLDAMKATGHDGETILLCQLCNAICQGLMQPW--VPPLAVPTAPGTPSKEKPDKADKGAKGRQSGKGVKGGASKANVQGVTIDPEGTNELKQALEVIEYSLNVTSGTEP------------------------KNQVPVGVRHHLIKTWVFCKQLLNQQIGKNLGHDD-ESPQSQGPMCKSLCALEMLSLQGNGLPEFQQSPTLPEVSEIVVGQYRVAFCSIFLGILLLNI 897 HSP 4 Score: 70.0922 bits (170), Expect = 1.301e-11 Identity = 26/73 (35.62%), Postives = 52/73 (71.23%), Query Frame = 2
Query: 3734 TSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFK 3952
++ +++L +R M+ +FQ + + W + L+++D+AI D+PRT+H+L +F+HR++TK +L ++ D+ KFK
Sbjct: 1050 SAYDEELMMRAAMYGVLFQAYADKCDWEQGLRAMDQAIKDMPRTHHRLLIFKHRVITKAKLGRNVLFDMGKFK 1122
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Medaka
Match: ENSORLT00000044229.1 (cilia- and flagella-associated protein 46-like [Source:NCBI gene;Acc:105355713]) HSP 1 Score: 71.2478 bits (173), Expect = 5.953e-12 Identity = 94/398 (23.62%), Postives = 158/398 (39.70%), Query Frame = 2
Query: 7313 YSEIMENLQEYLGPIFEKLGNFF---PPGN----ERNSPSNQKANKETISQPGKDSIIILTDEILSVASLESIPKLK----SSISRDFSLQVLHHRLK----GMGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLRE------QQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQI-----------RNPPIEEVPEL--IKNLSLTSPSESFIKSNLVLYGLPNIAVS 8404
+ E+++++ +YL P+ +L NF PP + + S + ++ + ++PG + ++IL D L LES+ L+ S +SRDFSLQ + RL ESD K K + A + K+T + P+ P+ ++ +D + VD + E + ++RE Q T W G E E L+ ++ IYV + F+ + P+ L +N + + FD + + S Q L Q+ A L+SL GV V NQW + N T L + G+ +Q + RNP + L K +T +F N +LYGLP + ++
Sbjct: 63 FREVVQDMDDYLNPLLTQL-NFSCMRPPASPFQGSEVTKSKAEEDEASTAEPG-EGLVILADRNLLELPLESLSILQEEGLSFVSRDFSLQFFYSRLSPDKTQKAESD---------NKTDTKLSKGAKGKAHQSQGIKATPVGPVQ---PSLAIPVDIQNFHYFVDS----------------EGERCLPLMREVLETHMQPFTHLWGG-------SRVSETERVLRRCSAFIYVGRDRFVGIIPPKRLAALNLPECRLALLFDHVHKQAAPHSQSNHDPHKSMQRLALQEPVETALLLSLSGVSCVVLNQWPCSLQQNALNAATVLDELLRVRKSSGQTIQTLRGGVNSAEPHHRNPGWRDTGRLNNSKEDGVTGTPSAF---NCILYGLPYLTIT 420
BLAST of cilia- and flagella-associated protein 46 vs. Planmine SMEST
Match: SMESG000014118.1 (SMESG000014118.1) HSP 1 Score: 4263.76 bits (11057), Expect = 0.000e+0 Identity = 2146/2192 (97.90%), Postives = 2155/2192 (98.31%), Query Frame = 2
Query: 785 SKKILSSLTGVDEVSISSSSETLNTKPDQKSTIVIPINSKKLTNEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEADFVNFXXXXXXXXXXNEKITPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCASLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKMRLWVDLVKLSRKVQLWDICRVASCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRAAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVGQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFXXXXXXXXXXXXXXXXXXKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNEFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLFLEPHTXXXXXXXXXXXXXKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNTEATKEKSLSHPNSSVKNRSRNNFRKPGSGLKPNVKNENIQETPEMXXXXXXXXXXXXXXXPTWFFLQEFRPRTLEDICDIRMLSKFVICLTTQAKLLGNSGGKSRDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTXXXXXXXXXXXXXXPNSEPVQSKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIFHHEKFINSPFVIPEYCAHVEIIKANLEEYKKQNKSQVLPMNFETQSTLPLKLSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLIVIEKCLEIYQIIFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKRNQWSRPLMDIEDPLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAIEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILLKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVKEKLNSTFPAWKMTNVSLNPLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPAAIGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQTEIQRAFLEKEALNANIQMNILEIEENYSEIMENLQEYLGPIF 7360
+KKILSSLTGVDE SISSSSETLNTKPDQKSTIVIPINSKKLTNEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQE N PATLKFRLKTIEKCKTTLLNVIRSENSRLIELGC SLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKM LWVDLVKLSRKVQLWDICRV+SCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLR+AALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTV QTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFPTETTNPVPSPNTNSNNNKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQN+FYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLF EPHTEEEGPR+EAELEEKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNTEATKEKSLSHPNSSVKNRSRNNFRKPGSGLKPNVKNENIQETPEMTNDTSNTDNNEELTNPTWFFLQEFRPRTLEDICDIRMLSKFVICLTTQ+KLLGNSGGKS DNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTKSKDKAAKKDKSAKPNSEPVQ KTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAI HHEKFINSPFVIPEYCAHVEIIKANLEEYKKQNKSQVLPMNFETQSTLPLKLSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKL+VIEKCLEIYQI FDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNK NQWSRPLMDIED LETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQA+EMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNIL KTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSV EKLNSTFPAWKMTNVSLN LELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPAA GDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQ EIQRAFLEKEALNANIQMNILEI+ENYSEIMENLQEYLGPI
Sbjct: 265 AKKILSSLTGVDEASISSSSETLNTKPDQKSTIVIPINSKKLTNEQRCSLLLELAKISLQKSIFDACHQCITKVQQMASEEMLPLLQEFKREN---------------NPATLKFRLKTIEKCKTTLLNVIRSENSRLIELGCVSLWNICLPILQPSLVLKIHSHLVLVADSLEMIGCLNYKLCAQVHIEVGKGYAALEQINDAIKYLQKAILLDRKKEYTELIEHFIHKLKMQEKLYEKPLDPCDEARQLLEQVRSTLNNEIIPIEKLRPLLAQIGHCLAPDDFLVILDHDLSMKEGENDKDVHGKFTKTFSWENVVTRINSYLLRSGNTDDKRKMSLWVDLVKLSRKVQLWDICRVSSCFAILYDDGRWKNHFQIKIENSSRKGNRNMGASSGMQNAETSAVETNQMSELDVTDSSINENKRRKEESDLSNNSMKQSRLEKVLLEQLTETYFIRAEAFIQALRNEGAQLLTFPERPKDLKKHPKGFIKDTSNEDKEWKSYCESLEKLSNQIVACFLRSAALSVDLQAPWFTQNAATYLWNYSMHILTLSDHGILVSSFQTMYDYLRQMINEKMVDIITNYGIVLAHGMIQKYMPIPVFLLDNIFDSPSASTNLDKKAKKDRESPKSKTVSQTGAGQNSTESVNQFKKALDICQYLMDITSDVPPFPTETTNPVPSPNTNSNNNKTAQINRTIDIKTKGQLIEIWVQCKQLLQQPVGRNLGTDDAEIKLGQRPIVRAMTSVQMVWICQNDFYKFPDLLSVNDCLQYIDLCDWEEASVLLNLLMKLAQITVKGSVSNSINTLVKITEKAVKVSPETHIGVVKRANKTNTNTGTVDAKLALSFIYFCHGLCLHKTGQNTSVLMQCLQLLVKACCYARDGNDYFLVSQISHYFWRISQVLIKRFSNTCQIAKEIEIIINCLSLTVDKKRLKLFFEPHTEEEGPRVEAELEEKLPHILQIGDDQKLITLDNNKALFLTSYRRQMSKTSLEQDLTIRIQMHIAIFQCFCFQEKWTEALKSLDEAIADLPRTNHKLDLFRHRLLTKVRLNHSIELDVQKFKNQKEEEVATLWREIAIVSNSRESQLFAYKKSIESLKNKSNNLLKVDLIMELSQWLFHSDYPIELCSQYIDFAINILLNTEATKEKSLSHPNSSVKNRSRNNFRKPGSGLKPNVKNENIQETPEMTNDTSNTDNNEELTNPTWFFLQEFRPRTLEDICDIRMLSKFVICLTTQSKLLGNSGGKSTDNLIMASGCVIRMWQVAMSSLTVAIKEIGKSKVEIESPTKSKDKAAKKDKSAKPNSEPVQIKTKVPKRKGPADWLPTSNEEWSVFEISDDALEAITSKVYMKSTGLHEISIPNIGQFMGACDALIEMLAEQGYELLTFPILSIMDAISRSDETVPAKLLNQYIHLRWLQFSLRINNQDAIIHHEKFINSPFVIPEYCAHVEIIKANLEEYKKQNKSQVLPMNFETQSTLPLKLSHLNRKLTKFGYIHETWIKIAWILVEIGYNQEASEVICVAEELSKMLNDEKMNTECNLLKAKLMINQGEFDEALLYIDAIGSCHFTFKQWSEIIDLLIEIGTDQANEHGDISKIRLRLLRMKDHTENRVAEIKLSNQQLNSDNLIGKMNYGLLLLDRRIATLDLGRSRNWENVSEMMEAFLGKLEEIESLHESNKSFKYLIEISMEKLEIFDFLMKVNEKTNFKSLLIKKLLVIEKCLEIYQIFFDDIYRVCSPLESSAFNLPIDRKLCRAKLMLSDCLIEMFSITVEDTAEKVLQHRAKDHLTQIVDEYVEEVIPDKESKEWRIVTNGICDKICSTLSDVHTICSQIPDINAQCLLKYGHVMKLRAMELYPNKPNQWSRPLMDIEDLLETQKSKLVNLQDSSNNKEIENNSYGLLENEDTEKNYQSSIKMSKAKSQMIQNRYYLNQAVEMINQSIAISIAHEFYDCVVTATELLIDIFGQFDCQLSGQLLCLHQNSRTAAQMRNILSKTLRKASMGQLAAFVLQRDYMIRKSKREESNLTEFSLYKSVMEKLNSTFPAWKMTNVSLNSLELLKDLSYLTSGFLIFEHSLNQQFLYCAFIDTKHKTANLSKPNNKPAATGDRTKICRIATSPSQIEDLQNYWRGFKSKLEKLVFNVENKTQIFEQRINLYSEIENQIEIQRAFLEKEALNANIQMNILEIQENYSEIMENLQEYLGPIL 2441 HSP 2 Score: 493.812 bits (1270), Expect = 1.251e-140 Identity = 236/240 (98.33%), Postives = 237/240 (98.75%), Query Frame = 3
Query: 87 LISKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDDGSVINEAKKFFPA 806
LISKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDDGSVINEAKK +
Sbjct: 32 LISKRNINECFEQATDMCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKPIGFILKAINFSKKEKRFHYLIYNSALIYYEIVRNYLRPNFYQYLCKSLSSICKTLEEVNDPNIQFRSQLMLLLIQCHIDAGHRKEVIQIVDGTATFLGKHSPYLLKDLLTTLINNSIGTLEKYLWYTKNHPAMQTFIRLCKLKIMKDKDDGSVINEAKKILSS 271 HSP 3 Score: 63.929 bits (154), Expect = 2.602e-9 Identity = 32/41 (78.05%), Postives = 32/41 (78.05%), Query Frame = -2
Query: 6 CLFKTFIYVSFRNQYLARAYLIQAQLIKPERFDEETALDKP 128
CL F NQYLARAYLIQAQLIKPERFDEETALDKP
Sbjct: 68 CLRLYFSLDPQENQYLARAYLIQAQLIKPERFDEETALDKP 108
BLAST of cilia- and flagella-associated protein 46 vs. Planmine SMEST
Match: SMESG000014116.1 (SMESG000014116.1) HSP 1 Score: 472.626 bits (1215), Expect = 3.924e-152 Identity = 263/279 (94.27%), Postives = 264/279 (94.62%), Query Frame = 2
Query: 7577 MGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV 8413
MGESDPVDAPSTTDIKKKDKSKTPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVDPSQDSSNSNSPSLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFL +QKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV
Sbjct: 1 MGESDPVDAPSTTDIKKKDKSKTPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVDPSQDSSNSNSPSLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFL---------------LQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV 264
BLAST of cilia- and flagella-associated protein 46 vs. Planmine SMEST
Match: SMESG000014116.1 (SMESG000014116.1) HSP 1 Score: 471.47 bits (1212), Expect = 9.396e-152 Identity = 263/279 (94.27%), Postives = 263/279 (94.27%), Query Frame = 2
Query: 7577 MGESDPVDAPXXXXXXXXXXXXXPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVXXXXXXXXXXXXXLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQFLCCFDRMETRKTKERIVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV 8413
MGESDPVDAPSTTDIKKKDKSKTPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVDPSQDSSNSNSPSLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQ IVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV
Sbjct: 1 MGESDPVDAPSTTDIKKKDKSKTPHRLAAFRDINKKSTKIIPLNRQLPTGSMAIDTNQMRFLVDPSQDSSNSNSPSLGSFFKNEMTISVLREQQITPRWVGLVGDDECPTTCELELYLKEANSMIYVNTENFISLFSPQLLLMINANDLQ----------------IVQKSSAMSKQDLRSQDSTSAAQLISLMGVKSFVFNQWAGLIDWNKWEIKTFFSGFLDDGMNVGECLQQIRNPPIEEVPELIKNLSLTSPSESFIKSNLVLYGLPNIAVSGSV 263 The following BLAST results are available for this feature:
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of cilia- and flagella-associated protein 46 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of cilia- and flagella-associated protein 46 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 4
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of cilia- and flagella-associated protein 46 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of cilia- and flagella-associated protein 46 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032947 ID=SMED30032947|Name=cilia- and flagella-associated protein 46|organism=Schmidtea mediterranea sexual|type=transcript|length=8424bpback to top protein sequence of SMED30032947-orf-1 >SMED30032947-orf-1 ID=SMED30032947-orf-1|Name=SMED30032947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2468bp MASEEMLPLLQEADFVNFELLMMESESLNEKITPATLKFRLKTIEKCKTTback to top protein sequence of SMED30032947-orf-2 >SMED30032947-orf-2 ID=SMED30032947-orf-2|Name=SMED30032947-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=225bp MCVVLGENCLKNDIKIIANNCLRLYFSLDPQENQYLARAYLIQAQLIKPEback to top Annotated Terms
The following terms have been associated with this transcript:
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