X1.A.G7.1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032913 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10Note: Hover over icons to view figure legend
Homology
BLAST of X1.A.G7.1 vs. TrEMBL
Match: V9XRT9 (X1.A.G7.1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 8.910e-16 Identity = 68/213 (31.92%), Postives = 107/213 (50.23%), Query Frame = 3 Query: 177 SPPNYNDIVKSPLTGPTLSIPPPQYEG---IAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGII---LFVLMIWDSSLKCGDVCFKNQKQT-ITYISNAIPI 794 SPP Y+ + L T I Q + V ++ E S + H I+G I + G + IDP+LP + +YP+S+ G+FMI+SG+ SI ++N K LI + I NILALI N IGIL AI Y + ++ + G IF V +GI+ L VL+I++S+L C ++C + Q+ + + Y++ P+ Sbjct: 16 SPPEYSQQPTNLLINST-GIHSDQVQDGPVRLVLLESKEIRSGSKFINSKSHRIFGYILIACGIFVLCIDPLLPILLEYPFSISIAGVFMIISGIFSIQAVKRNLKLYLIFAKILNILALI----NIGIGILV---------------AILYGTAFYHRQ--NGILIFTVFYGILTMSLLVLLIFNSALICKEICCRQQRMSQVQYVACEQPV 206
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046815.1 (SMESG000046815.1) HSP 1 Score: 483.411 bits (1243), Expect = 5.453e-175 Identity = 237/242 (97.93%), Postives = 241/242 (99.59%), Query Frame = 3 Query: 87 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKNQKQTITYISNAIPIVPVGHI 812 MEQS+AGFTTEE+AIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMI+SGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFK QKQTITYISNA+PIVPVGHI Sbjct: 1 MEQSNAGFTTEEYAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKKQKQTITYISNALPIVPVGHI 242
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046819.1 (SMESG000046819.1) HSP 1 Score: 483.411 bits (1243), Expect = 7.173e-175 Identity = 236/242 (97.52%), Postives = 241/242 (99.59%), Query Frame = 3 Query: 87 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKNQKQTITYISNAIPIVPVGHI 812 MEQS+AGFTTEE+AIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMI+SGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKG AIFNVVFG+ILFVLMIWDSSLKCGDVCFKNQKQTITYISNAIPIVP+GHI Sbjct: 1 MEQSNAGFTTEEYAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGLAIFNVVFGMILFVLMIWDSSLKCGDVCFKNQKQTITYISNAIPIVPIGHI 242
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046812.1 (SMESG000046812.1) HSP 1 Score: 474.552 bits (1220), Expect = 1.802e-171 Identity = 232/242 (95.87%), Postives = 236/242 (97.52%), Query Frame = 3 Query: 87 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKNQKQTITYISNAIPIVPVGHI 812 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPL GPTLSIPPPQYEGI VPPQTTEESQNSMLRYKRMHLIYGTI VVLGGIQVAIDPILPSIFQYPYSMYPGGLFMI+SGLCSIYGSRKNNKCSLIASLIFNILA+IVIFTNYIIGILA NQSGF FTPCNQYN+ICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFK QKQTITYISNA+PIVPVGHI Sbjct: 1 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLNGPTLSIPPPQYEGIVVPPQTTEESQNSMLRYKRMHLIYGTIYVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILAMIVIFTNYIIGILALNQSGFSFTPCNQYNSICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKKQKQTITYISNALPIVPVGHI 242
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046804.1 (SMESG000046804.1) HSP 1 Score: 409.068 bits (1050), Expect = 8.800e-146 Identity = 203/228 (89.04%), Postives = 210/228 (92.11%), Query Frame = 3 Query: 87 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEGIAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKNQKQTIT 770 MEQSDAGFTTEEHAII NSDRH QT++PLS NYNDIVKSPL GPTLSIPPPQYEGI VPPQTTEESQNSMLRYKRMHLIYG ISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMI+SGLCSIYGSRKNNKCSLIASLIFNILALIVI TN+II L NQSGF FTPCNQYN+ICYPSVSFYTEGVKG AIFNVVFG+ILFVLMI D +LKC DVCFKNQKQTIT Sbjct: 1 MEQSDAGFTTEEHAIIPNSDRHYFQTYQPLSLSNYNDIVKSPLNGPTLSIPPPQYEGITVPPQTTEESQNSMLRYKRMHLIYGIISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILALIVISTNFIISFLVLNQSGFSFTPCNQYNSICYPSVSFYTEGVKGLAIFNVVFGMILFVLMICDFNLKCIDVCFKNQKQTIT 228
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046817.1 (SMESG000046817.1) HSP 1 Score: 224.942 bits (572), Expect = 5.621e-73 Identity = 113/241 (46.89%), Postives = 162/241 (67.22%), Query Frame = 3 Query: 87 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEG-------IAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIVSGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKNQKQ-TITYISNA 785 M + + GFTT+E+A + QTF+P +PPNYNDI+K P + +PPP+Y+G I V E QN +LR+K+ HL G + ++GGIQ+ +DP+LP++F YPYS++PGGLFM++SG+CSI+GSR+ ++CSL ASL F+ILAL+++F II I+ C +CYPSV FY+ G+KGF+IFN+++GIILFVL+ WDS +KCGDVC ++Q +TY N Sbjct: 1 MARPNEGFTTDEYATVSEPVPPYPQTFQPPNPPNYNDIIKIPTDVNVMYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVILRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFDILALVLVFIAIIIAIVVIIAGDSILF-CKSSIYLCYPSVDFYSSGLKGFSIFNLIYGIILFVLLCWDSGIKCGDVCCCKRRQPRVTYNPNG 240 The following BLAST results are available for this feature:
BLAST of X1.A.G7.1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of X1.A.G7.1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of X1.A.G7.1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032913 ID=SMED30032913|Name=X1.A.G7.1|organism=Schmidtea mediterranea sexual|type=transcript|length=897bpback to top protein sequence of SMED30032913-orf-1 >SMED30032913-orf-1 ID=SMED30032913-orf-1|Name=SMED30032913-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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