SMED30032845

Overview
NameSMED30032845
Smed IDSMED30032845
Length (bp)3486
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032845 (SMED30032845) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032845 (SMED30032845) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032845 (SMED30032845) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032845 (SMED30032845) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032845

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30032845SMESG000036221.1 dd_Smed_v4_16440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30032845SMESG000036221.1 dd_Smed_v4_16440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
dorsal-ventral muscle cellSMED30032845SMESG000036221.1 dd_Smed_v4_16440_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032845 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002183:22862..35956 -17108
Homology
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000036221.1 (SMESG000036221.1)

HSP 1 Score: 1925.21 bits (4986), Expect = 0.000e+0
Identity = 1072/1079 (99.35%), Postives = 1075/1079 (99.63%), Query Frame = 1
Query:   79 AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQFLIPHENFEFKTSTEEQLDISSRVFSSQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQXXXXXXXXXXXXXSNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDXXXXXXXXXXXXXXXXISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPXXXXXXXXXXXXXXXXXXXXXXLNKQLTVTSAKSIDTNTLFLTMKXXXXXXXXXXXXXXXXXXXXXRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQXXXXXXXXXXXXXXXXXXXXXXXQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHXXXXXXXXXXXXXXXQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKESTSNHTNSPNINNYSTSH 3315
            AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQF IPHENFEFKTSTEEQLDISSRVF+SQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKIT+TKKEILIEESNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSK+SYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILL FNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPIITKSQSTSTETSKISS STKSLNKQLTVTSAKSIDTNTLFLTMKILIGLVICVVIIIILNVVIKIRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQNSNNQHPPLLPPPGPLYNSSNSSQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHDSDSLASGSGSKTSSQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKES SNHTNSPNINNYSTSH
Sbjct:   16 AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQFQIPHENFEFKTSTEEQLDISSRVFASQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKITDTKKEILIEESNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSKRSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLTFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPIITKSQSTSTETSKISSLSTKSLNKQLTVTSAKSIDTNTLFLTMKILIGLVICVVIIIILNVVIKIRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQNSNNQHPPLLPPPGPLYNSSNSSQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHDSDSLASGSGSKTSSQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKESASNHTNSPNINNYSTSH 1094          
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 160.999 bits (406), Expect = 1.098e-39
Identity = 152/672 (22.62%), Postives = 305/672 (45.39%), Query Frame = 1
Query:   94 INITPLRSGFVKYPGEFSDNYE-IIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLG-TNSKIFDRKYCQMDVNKDTSVCSVQFLIPHENFEFKTSTEEQLDISSRVFS--SQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQXXXXXXXXXXXXXSNSLENSG-----------GQRIVELLVYLRIHHLNSIPKKSD---NWISCKIIQASFDXXXXXXXXXXXXXXXXISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPF-VRKTSNE-ISKKIIRKPD----------WNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYK-----NSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLL----QSVQIGSSEISIFDNHSSK-IAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNL 1989
            I+I+P  + F  Y  +     +   + NP  +  N++    +  K       ++  ++ TDK ++       T++ T+  IC+F +   + + +  +CC + ++    T++  +  K+C+  +++ +S C +  +   ++F  K S+ +      R F+  +    C+S +  +  LA+     L+    L V W  I  ++    L IG P  S++P S   FP+K+ S  +++ + +QC    +L++ S+ FD   F    +      + I+      LEN G            + ++  ++Y RI     + +K+D    W SC+II           +  +   +  + I +N   F I PILK   +FSI + +  PI +  + + + S H++ D SF D+T +  C  +  S + CP  + K+S++    K   K D          ++++L +++WK  +  PI LW A DW+L    + +NKI+GW             C  +FQ +R K+L R++      +K +YG +  +  P LD+T  + K +I+ S ++  +     F  L    QS  +   E+ +    S + +A + ++    +V I +++  C+  + + Q  +     + ++ V   L  D+ + CY  I++  +D+ +  L
Sbjct:   24 ISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQY--YDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMF-QKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITIL---LENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQT-VHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKVINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQS-DVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRL 687          
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000067886.1 (SMESG000067886.1)

HSP 1 Score: 73.559 bits (179), Expect = 8.705e-13
Identity = 73/319 (22.88%), Postives = 152/319 (47.65%), Query Frame = 1
Query: 1234 IGDESFSDITQTTECQPVSPSVLKCPF-VRKTSNEISKKI--IRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYKNSNYQ--------CEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIP---LLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQT--INSNDLPFHKILVS-QRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTEC 2139
            I D+S  D+T    CQ    S LKCP  +   ++E S+ I   +  +W++ L L SWK  + +  ++   + W ++ +D  LNKI+GW    +  +        C+ ++Q+T  K+  R+        K Y   S++++ P   +LD+T  +   ++ ++    ++    +++ LQ ++ G  ++ + D +++ +    ++    +V + D+EVSC   +++     + S  +  H I +S  R ++  ++ C   I LIF DN + N+       F +K I     +++ +L    Y   ++     F  +++ES  C
Sbjct:  377 IFDDSIIDVTDKLHCQSFDKSKLKCPSNMESYTDEQSRYINNYKSLEWSIELKLGSWKTVLDIKPQIINENFWEISISDYVLNKINGWRGPENRIRSALLKDETCKLLYQITPIKIFRRV--------KMY---SDINQDPVEKMLDVTNQIDIASLSITPKIVSIHKYGNYLFLQGIKEGDFKLDVKDYNNNVMVSKSLKVLGDKVYVKDIEVSCYPSINLTSKSLVFSFLVNIHYINMSFNRFNK--SKDCVISIDLIFTDNSRCNIFTIPNNQFFVKFITNREVLEVFELNDMFYNYFKFNNNTSFYVEIMESKMC 682          
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000068172.1 (SMESG000068172.1)

HSP 1 Score: 69.707 bits (169), Expect = 1.173e-11
Identity = 95/385 (24.68%), Postives = 170/385 (44.16%), Query Frame = 1
Query: 1129 IFPILKRSIYFSILDTVMGP-IEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKT--SNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWN-YKNSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELD--KIPLLDMTPYLRKQNIKLSTNN-FNVD--LRNDFMLLQSVQIG-SSEISIFDNHS-SKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINS----NDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQ-----YKEPGKFIFQLLESTEC-LFRLIFQKDVNIYENTKSTNPIDPSLQ 2220
            I P++K S    I      P I+   + L++    N  D S   I+   +  PV P  L      ++  S +++ +    P W++ +       T+  P+ +W+ + W+L  +D  L  I  W  Y+  + +C P ++V R K++ RL       +K Y G  + D  +IP +D+T   +   IK    + F+++  L  ++ L+ + Q   SS I +F  +S + +    I AD Q +S+VD+ V+CI  + + Q   S    N   ++K +     S+  ++ CY  + + F+D     L     T +QL L +        D + FIY  +       +   +    L E   C   RLIF K +  +   + +N I P+ Q
Sbjct:  370 ILPVVKES--RDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNP-WSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQIKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTN-------PDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIE-WVTVEPSNNIVPNTQ 743          
The following BLAST results are available for this feature:
BLAST of SMED30032845 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032845 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000036221.10.000e+099.35SMESG000036221.1[more]
SMESG000014253.11.098e-3922.62SMESG000014253.1[more]
SMESG000067886.18.705e-1322.88SMESG000067886.1[more]
SMESG000068172.11.173e-1124.68SMESG000068172.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032845 ID=SMED30032845|Name=SMED30032845|organism=Schmidtea mediterranea sexual|type=transcript|length=3486bp
TCTTAATAGAAAATTGCAATCCTATTATAAAAAATGGAAATATTATTTTT
GTTAATTTGGATTTTAATACCATTTCTAGCAGAATGCACAACTATAAATA
TTACGCCTCTTCGATCTGGATTTGTCAAATATCCGGGTGAATTTAGCGAT
AACTATGAAATTATCTATAAAAATCCATTGGAAAATAAAATGAATATCTC
TGTATTGTTTGGGAATTTGACGAAAACCAAAAATTTAGAACCTCTAAATT
ATATAACAAATGTTTTTACGGACAAAACGAGATTCTCCATTCAATCAAAT
CAACTGACTGACACCTCGACAGTTTATGCAATTTGTCAATTCACTCATGG
AAACTCCAGACCCTTTGAATACAACAAGGTTTGCTGCGAAATGATTCTTA
AAAGGCTGGGAACAAACAGTAAAATCTTCGACCGAAAATACTGTCAAATG
GACGTCAATAAAGATACGAGTGTTTGTTCGGTTCAGTTTCTAATTCCTCA
TGAAAATTTCGAATTTAAAACCTCCACCGAAGAGCAATTGGATATTTCTT
CGAGGGTTTTTTCATCACAAGATGGAAAATGTTCCTCAACGGCCATCGGA
GTGAGCAATTTGGCGCTTGGCTATCCAACAAAGTTGACTATCATGCAAGA
TTTACTGGTGAAATGGAAAATAATCGACTCAACAGATTCATTCGGAGAAT
TATTAATCGGTCTTCCTTTGGATTCAATGGAACCGGGATCAAAGTCCATT
TTCCCATTGAAAATTAATTCAACAGCGGTTTACAACAGTCTCGCAATCCA
ATGTTCCCATGGCCGAAGTCTCAAGCTATCGTCGATTTCATTCGACTCAG
TGAGATTTACTGGCACTTTACAGAAAATTACTGAAACCAAGAAAGAGATC
CTGATTGAGGAAAGTAATAGCTTAGAAAATTCCGGTGGTCAACGCATCGT
TGAATTGCTGGTGTATTTGAGAATTCATCATTTGAATTCGATTCCCAAAA
AATCAGACAATTGGATTAGCTGTAAAATAATTCAGGCCTCTTTCGATAAA
AACAAGTCGAAAAAATCCCGAAAAACAACAGAAAAAGAAAAGAAAATTTC
CATTCAAGTCAATATTGGACATTTTTATATTTTTCCAATTTTAAAGAGGT
CAATTTATTTCTCTATTCTTGACACAGTCATGGGTCCAATAGAAATGGGG
AAAAATGATCTGAAGATTTTATCTGCTCATAATATTGGAGATGAATCGTT
TTCAGATATCACACAAACAACAGAGTGTCAACCAGTCTCACCGTCGGTAT
TGAAGTGTCCATTCGTTAGAAAAACCAGCAATGAGATTTCAAAGAAAATT
ATTCGAAAGCCCGATTGGAATGTCGTTCTATCACTAAGAAGCTGGAAGAA
GACGGTCACGGTACCAATTGAATTGTGGCAAGCAGATGACTGGAGACTCA
CCACAACTGATCGTACTTTAAATAAAATTTCTGGTTGGAATTATAAAAAC
TCAAATTATCAATGTGAACCCGTATTTCAAGTAACAAGATGTAAATTATT
AGCAAGACTTATGAAAGATACTTTTGATTCTAAAAAGTCCTATTATGGTC
AAAGTGAATTAGACAAAATTCCATTGCTCGACATGACTCCTTACCTGAGA
AAGCAAAATATTAAATTATCAACCAACAATTTTAATGTTGATTTGAGAAA
CGATTTCATGCTTCTTCAAAGTGTCCAAATTGGTTCTTCTGAAATTTCTA
TCTTTGATAATCACAGTAGTAAAATTGCATTTGACTTCATCCAAGCTGAT
GCTCAAGAAGTGTCAATAGTTGATATGGAGGTTTCTTGCATCCATGATGT
GCACATTAATCAGACAATCAATTCCAATGATCTTCCATTTCACAAGATTC
TTGTCAGCCAGAGGCTTTCAGAAGACTCGACTCAAGCTTGCTATTTTCAG
ATTCTGTTGATTTTTAATGATAACAAAAAATCAAACTTATTAGACTACAA
ATTTACAAATTTCCAATTGAAATTGATCCACACAGAAAGATCCATCGATA
TCAAGGATCTGGAAAGCTTCATTTATTATCCATCCCAATATAAAGAGCCA
GGAAAATTTATTTTTCAGCTGCTAGAATCGACTGAATGTTTATTCAGATT
GATTTTCCAAAAGGATGTAAATATTTATGAAAATACAAAGTCAACAAATC
CCATTGATCCTTCTCTTCAATCACCGATAATCACTAAATCCCAATCCACT
TCCACAGAGACTTCCAAAATTTCCTCCCCTTCGACAAAGTCCTTGAATAA
ACAACTCACAGTAACCTCAGCAAAAAGTATCGATACAAACACTTTGTTTT
TAACAATGAAAATACTAATTGGATTGGTCATTTGTGTAGTGATAATTATT
ATTTTGAATGTTGTGATAAAAATTCGCAACCGTAAAAGTGCTTCTCTTCT
TGATAGACATTTAAATAACTGCCAAATCTATGGTCAAGTTGAAAAGTTAA
CTACAAGTGCCTACTTGCCGCTGGAAATTACGAGTCAAAATTCCAACAAC
CAGCACCCACCTCTCCTTCCACCTCCGGGACCTTTATATAACTCATCGAA
TTCATCGCAAATGGAATTAACTGTCAATGCTACTCCCGATAGGTTTTGGG
ATTTGCCTCACACAGTTGACAAATCTGAGCGTAAAATGACAACTAATCAC
ATGGTATTAGGAATGCAAATGAATCCTTTCACTGGTCAAGGCCATTCGAT
ACCATTTCCAAATGTATTTTCCAACAGTCGGACTTCGTCATCTCCACATA
GTGATCACATTGAATTTGGTTATACAAACTCTAGTGGTTCAAAAATATCT
CACTCAAGAGCTTTGCAACAGTCTCAAAACGGATTCAGTTATCCACCCGG
TTCCATTCATGATAGCGATTCATTAGCTTCCGGATCCGGCAGTAAGACAT
CCAGTCAAATCGCAGTTATTGTTCCTCCATGTCCTCCACTTCGATGGAAT
AACAACTGGAATTCAAATCAATTTCCCGGTTATTTCCCTAATGACAAAGA
TACAAACCTAGATCCTTCAATATCACAGTTTTCAAATGAGCAAAGCAGCC
AAATAAGCTTAACAAACAGTCACAGCAGTTCTTGGGAAATTAAGCAGCCT
TTGGTTTTACCCCCAATGGGGTTTAATTCAATTGAAAATGTCTGTGAAAA
AAGTAGCGGCGTTGGAAGTCTAACTAGCACGCAGCCGGGATCCGATTGCA
GCAAAAAAGAATCCACATCTAACCACACTAATTCTCCCAATATAAATAAT
TACTCCACATCTCATTAAAAAATCTCCGAAAAAAATTAATTCCAACTGAC
AAATTTTCGGGGAAATTAAAACTATTCTAGCATTTCAGTTGGAATCGAGT
TGATTTGACTCTCCCACTTGGCATAATTGAATGAAGGGACAGTTGAGTGT
ATTGCGGTTTCTAAATCACATTCTGTACAAATATTC
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protein sequence of SMED30032845-orf-1

>SMED30032845-orf-1 ID=SMED30032845-orf-1|Name=SMED30032845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1095bp
MEILFLLIWILIPFLAECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLE
NKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAI
CQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSV
QFLIPHENFEFKTSTEEQLDISSRVFSSQDGKCSSTAIGVSNLALGYPTK
LTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVY
NSLAIQCSHGRSLKLSSISFDSVRFTGTLQKITETKKEILIEESNSLENS
GGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKKSRKTTE
KEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHN
IGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLS
LRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYKNSNYQCEPVFQV
TRCKLLARLMKDTFDSKKSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNF
NVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEV
SCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKS
NLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLEST
ECLFRLIFQKDVNIYENTKSTNPIDPSLQSPIITKSQSTSTETSKISSPS
TKSLNKQLTVTSAKSIDTNTLFLTMKILIGLVICVVIIIILNVVIKIRNR
KSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQNSNNQHPPLLPPPGP
LYNSSNSSQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFT
GQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNG
FSYPPGSIHDSDSLASGSGSKTSSQIAVIVPPCPPLRWNNNWNSNQFPGY
FPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSI
ENVCEKSSGVGSLTSTQPGSDCSKKESTSNHTNSPNINNYSTSH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0002028dorsal-ventral muscle cell
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR031437TMEM132_M
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031437Transmembrane protein family 132, middle domainPFAMPF16070TMEM132coord: 464..665
e-value: 1.7E-10
score: 40.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1059..1094
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.895
NoneNo IPR availableTMHMMTMhelixcoord: 773..795