SMED30032845
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032845 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30032845 aligns in the following genomic locations:
Homology
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000036221.1 (SMESG000036221.1) HSP 1 Score: 1925.21 bits (4986), Expect = 0.000e+0 Identity = 1072/1079 (99.35%), Postives = 1075/1079 (99.63%), Query Frame = 1 Query: 79 AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQFLIPHENFEFKTSTEEQLDISSRVFSSQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQXXXXXXXXXXXXXSNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDXXXXXXXXXXXXXXXXISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPXXXXXXXXXXXXXXXXXXXXXXLNKQLTVTSAKSIDTNTLFLTMKXXXXXXXXXXXXXXXXXXXXXRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQXXXXXXXXXXXXXXXXXXXXXXXQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHXXXXXXXXXXXXXXXQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKESTSNHTNSPNINNYSTSH 3315 AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQF IPHENFEFKTSTEEQLDISSRVF+SQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKIT+TKKEILIEESNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSK+SYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILL FNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPIITKSQSTSTETSKISS STKSLNKQLTVTSAKSIDTNTLFLTMKILIGLVICVVIIIILNVVIKIRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQNSNNQHPPLLPPPGPLYNSSNSSQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHDSDSLASGSGSKTSSQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKES SNHTNSPNINNYSTSH Sbjct: 16 AECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLGTNSKIFDRKYCQMDVNKDTSVCSVQFQIPHENFEFKTSTEEQLDISSRVFASQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQKITDTKKEILIEESNSLENSGGQRIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKKSRKTTEKEKKISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWNYKNSNYQCEPVFQVTRCKLLARLMKDTFDSKRSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLTFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTECLFRLIFQKDVNIYENTKSTNPIDPSLQSPIITKSQSTSTETSKISSLSTKSLNKQLTVTSAKSIDTNTLFLTMKILIGLVICVVIIIILNVVIKIRNRKSASLLDRHLNNCQIYGQVEKLTTSAYLPLEITSQNSNNQHPPLLPPPGPLYNSSNSSQMELTVNATPDRFWDLPHTVDKSERKMTTNHMVLGMQMNPFTGQGHSIPFPNVFSNSRTSSSPHSDHIEFGYTNSSGSKISHSRALQQSQNGFSYPPGSIHDSDSLASGSGSKTSSQIAVIVPPCPPLRWNNNWNSNQFPGYFPNDKDTNLDPSISQFSNEQSSQISLTNSHSSSWEIKQPLVLPPMGFNSIENVCEKSSGVGSLTSTQPGSDCSKKESASNHTNSPNINNYSTSH 1094
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1) HSP 1 Score: 160.999 bits (406), Expect = 1.098e-39 Identity = 152/672 (22.62%), Postives = 305/672 (45.39%), Query Frame = 1 Query: 94 INITPLRSGFVKYPGEFSDNYE-IIYKNPLENKMNISVLFGNLTKTKNLEPLNYITNVFTDKTRFSIQSNQLTDTSTVYAICQFTHGNSRPFEYNKVCCEMILKRLG-TNSKIFDRKYCQMDVNKDTSVCSVQFLIPHENFEFKTSTEEQLDISSRVFS--SQDGKCSSTAIGVSNLALGYPTKLTIMQDLLVKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSISFDSVRFTGTLQXXXXXXXXXXXXXSNSLENSG-----------GQRIVELLVYLRIHHLNSIPKKSD---NWISCKIIQASFDXXXXXXXXXXXXXXXXISIQVNIGHFYIFPILKRSIYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPF-VRKTSNE-ISKKIIRKPD----------WNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYK-----NSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIPLLDMTPYLRKQNIKLSTNNFNVDLRNDFMLL----QSVQIGSSEISIFDNHSSK-IAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNL 1989 I+I+P + F Y + + + NP + N++ + K ++ ++ TDK ++ T++ T+ IC+F + + + + +CC + ++ T++ + K+C+ +++ +S C + + ++F K S+ + R F+ + C+S + + LA+ L+ L V W I ++ L IG P S++P S FP+K+ S +++ + +QC +L++ S+ FD F + + I+ LEN G + ++ ++Y RI + +K+D W SC+II + + + + I +N F I PILK +FSI + + PI + + + + S H++ D SF D+T + C + S + CP + K+S++ K K D ++++L +++WK + PI LW A DW+L + +NKI+GW C +FQ +R K+L R++ +K +YG + + P LD+T + K +I+ S ++ + F L QS + E+ + S + +A + ++ +V I +++ C+ + + Q + + ++ V L D+ + CY I++ +D+ + L Sbjct: 24 ISISPYLNAFKIYKKQSDFTLKSFAFINPAVHNFNLTTKLEDDIKDNQFIKSDFSVSIHTDKHQY--YDLDKTESYTINTICKFDYIPEKIYLFRNICCRITIQDFNFTSNDFYSIKFCKFSLSRHSSACFIDLMF-QKSFIPKPSSADHFYFGLRAFAVATNSDHCTSHSDDMRILAVSKLYPLSFSNGLSVFWTNIFQSNLNFSLEIGRPFYSIDPDSFVSFPIKLISDKMFSQMNLQCIISENLEIKSVFFDENIFDAQYKIAALPNRFIITIL---LENKGMNYLKSSQWWVDRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKLQGESKSVAYTSKILQT-VHIAINTDKFIIIPILKTRYFFSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLSMMKSSDQYFHTKFDNKSDNGRTRILPNKFDLILEMKTWKTVLFAPINLWVAQDWKLDINVKVINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDEKFSELIVDPQS-DVNDVEVRLLSVDSKRLLAIERVKIRLDKVIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKMCYLFIVVKLSDSSEIRL 687
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000067886.1 (SMESG000067886.1) HSP 1 Score: 73.559 bits (179), Expect = 8.705e-13 Identity = 73/319 (22.88%), Postives = 152/319 (47.65%), Query Frame = 1 Query: 1234 IGDESFSDITQTTECQPVSPSVLKCPF-VRKTSNEISKKI--IRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWNYKNSNYQ--------CEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELDKIP---LLDMTPYLRKQNIKLSTNNFNVDLRNDFMLLQSVQIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQT--INSNDLPFHKILVS-QRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQYKEPGKFIFQLLESTEC 2139 I D+S D+T CQ S LKCP + ++E S+ I + +W++ L L SWK + + ++ + W ++ +D LNKI+GW + + C+ ++Q+T K+ R+ K Y S++++ P +LD+T + ++ ++ ++ +++ LQ ++ G ++ + D +++ + ++ +V + D+EVSC +++ + S + H I +S R ++ ++ C I LIF DN + N+ F +K I +++ +L Y ++ F +++ES C Sbjct: 377 IFDDSIIDVTDKLHCQSFDKSKLKCPSNMESYTDEQSRYINNYKSLEWSIELKLGSWKTVLDIKPQIINENFWEISISDYVLNKINGWRGPENRIRSALLKDETCKLLYQITPIKIFRRV--------KMY---SDINQDPVEKMLDVTNQIDIASLSITPKIVSIHKYGNYLFLQGIKEGDFKLDVKDYNNNVMVSKSLKVLGDKVYVKDIEVSCYPSINLTSKSLVFSFLVNIHYINMSFNRFNK--SKDCVISIDLIFTDNSRCNIFTIPNNQFFVKFITNREVLEVFELNDMFYNYFKFNNNTSFYVEIMESKMC 682
BLAST of SMED30032845 vs. Planmine SMEST
Match: SMESG000068172.1 (SMESG000068172.1) HSP 1 Score: 69.707 bits (169), Expect = 1.173e-11 Identity = 95/385 (24.68%), Postives = 170/385 (44.16%), Query Frame = 1 Query: 1129 IFPILKRSIYFSILDTVMGP-IEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKT--SNEISKKIIRKPDWNVVLSLRSWKKTVTVPIELWQAXXXXXXXXXXXXNKISGWN-YKNSNYQCEPVFQVTRCKLLARLMKDTFDSKKSYYGQSELD--KIPLLDMTPYLRKQNIKLSTNN-FNVD--LRNDFMLLQSVQIG-SSEISIFDNHS-SKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINS----NDLPFHKILVSQRLSEDSTQACYFQILLIFNDNKKSNLLDYKFTNFQLKLIHTERSIDIKDLESFIYYPSQ-----YKEPGKFIFQLLESTEC-LFRLIFQKDVNIYENTKSTNPIDPSLQ 2220 I P++K S I P I+ + L++ N D S I+ + PV P L ++ S +++ + P W++ + T+ P+ +W+ + W+L +D L I W Y+ + +C P ++V R K++ RL +K Y G + D +IP +D+T + IK + F+++ L ++ L+ + Q SS I +F +S + + I AD Q +S+VD+ V+CI + + Q S N ++K + S+ ++ CY + + F+D L T +QL L + D + FIY + + + L E C RLIF K + + + +N I P+ Q Sbjct: 370 ILPVVKES--RDIFSIYSKPNIDFQNDPLQLYIITNYRDRSVKKISSKFQMYPVDPIHLSVHSNNQSLMSMDMNYQTYLNP-WSIQVQFIDNLHTLISPVRVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQIKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISADQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTN-------PDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKIE-WVTVEPSNNIVPNTQ 743 The following BLAST results are available for this feature:
BLAST of SMED30032845 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032845 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032845 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032845 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032845 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032845 ID=SMED30032845|Name=SMED30032845|organism=Schmidtea mediterranea sexual|type=transcript|length=3486bpback to top protein sequence of SMED30032845-orf-1 >SMED30032845-orf-1 ID=SMED30032845-orf-1|Name=SMED30032845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1095bp MEILFLLIWILIPFLAECTTINITPLRSGFVKYPGEFSDNYEIIYKNPLEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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