SMED30032843
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032843 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30032843 aligns in the following genomic locations:
Homology
BLAST of SMED30032843 vs. Planmine SMEST
Match: SMESG000024319.1 (SMESG000024319.1) HSP 1 Score: 1012.68 bits (2617), Expect = 0.000e+0 Identity = 550/571 (96.32%), Postives = 562/571 (98.42%), Query Frame = -3 Query: 90 MTNTARENLVNSLPVLKDKLFIKTGENSDKSINKILLRNNIDNITFSDGLFYATNKVTEFDFSTGCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPKIIERISKNSYYYTSCSEMLLVLKKHKLQLEYDSLNNIIDPGVILKINSFYKFIEKNSDNIKCQIDYIKYIKYIXXXXXXXXXXXXXXXXXXNAVSFISNILNFKIKLLNRGVEKCFYXXXXXXXXXSDLGAAVVEXXXXXXXXXXXXXXXXIPKFALPLKPKTMLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPVESNQEKSCISDFLTTTRSSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIKSDISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLEKLVMCDSVLCPLNIKNQHWVLFVYIKSTSESFVME 1802 MTNTARENLVNSLPVLKDKLFIKTGENSDKSINK+LLRNNIDNITFSDGLFYATNKVTEFDFSTGCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPKIIERISKNSYYYTSCSEMLLVLKKHKLQLEYDSLNNIIDPGVILKINSFYKFIEKNSDNIKCQIDYIKYI ENKSTFKSSNKNDNSSQNAVSFISNILNFKIKLLNRGVEKCFYSKSSKSICSSDLGAAVVELIDFFKEILLKKFKEEIPKFALPLKPKTMLNSCIQ+DNLENSVLAVKSPSVGMVQ+SVDSHLHLSDKFTVPVESN+ +SC+SDFLTT R STVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEI+ DISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLE+LV CD++LCPLNIKNQHWVLFVYIKS+SESFVM+ Sbjct: 1 MTNTARENLVNSLPVLKDKLFIKTGENSDKSINKLLLRNNIDNITFSDGLFYATNKVTEFDFSTGCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPKIIERISKNSYYYTSCSEMLLVLKKHKLQLEYDSLNNIIDPGVILKINSFYKFIEKNSDNIKCQIDYIKYIN---ENKSTFKSSNKNDNSSQNAVSFISNILNFKIKLLNRGVEKCFYSKSSKSICSSDLGAAVVELIDFFKEILLKKFKEEIPKFALPLKPKTMLNSCIQIDNLENSVLAVKSPSVGMVQVSVDSHLHLSDKFTVPVESNRGRSCVSDFLTTPRPSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIRGDISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLERLVKCDTILCPLNIKNQHWVLFVYIKSSSESFVMD 568
BLAST of SMED30032843 vs. Planmine SMEST
Match: SMESG000062936.1 (SMESG000062936.1) HSP 1 Score: 490.345 bits (1261), Expect = 6.694e-157 Identity = 239/246 (97.15%), Postives = 244/246 (99.19%), Query Frame = -3 Query: 90 MLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPVESNQEKSCISDFLTTTRSSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIKSDISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLEKLVMCDSVLCPLNIKNQHWVLFVYIKSTSESFVME 827 MLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPVESNQEKSCISDFLTT RSSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIKSDISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLE+LV CD++LCPLNIKNQHWVLFVYIKS+SESFVM+ Sbjct: 1 MLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPVESNQEKSCISDFLTTPRSSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIKSDISEVGTEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLERLVKCDTILCPLNIKNQHWVLFVYIKSSSESFVMD 246
BLAST of SMED30032843 vs. Planmine SMEST
Match: SMESG000035969.1 (SMESG000035969.1) HSP 1 Score: 334.724 bits (857), Expect = 2.304e-107 Identity = 225/423 (53.19%), Postives = 287/423 (67.85%), Query Frame = -3 Query: 90 WGPKIIERISKNSYYYTSCSEMLLVLKKHKLQLEYDSLNNIIDPGVILKINSFYKFIEKNSDNIKCQIDYIKYIKYIXXXXXXXXXXXXXXXXXXNAVSFISNILNFKIKLLNRGVEKCFYXXXXXXXXXSDLGAAVVEXXXXXXXXXXXXXXXXIPKFALPLKPKTMLNSCIQVDNLENSVLAVKSPSVGMVQMS-VDSHLHLSDKFTVPVESN----QEKSCISD--FLTTTRSSTVKA-SENSDLSV-VLKTAQSSTIMPVIETTEHNSVEIKSDISEVG-TEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLEKLVMCDSVLCPLNIKNQHWVLFVYIKSTSESFVME 1328 WGPKII I+ S+Y+ +CSEMLLVL+KHKLQLEYDSLNN+IDP VI KINS YKFIEKNSD+IKCQ + Y + E FKS N + + NAVSF NIL FKI LLNRG+ KCFYSKS+K+ C D G + ELIDF LLKKFK+EIPKFALPLKP+T+ ++ +V + V +VK +++ V + ++S+ V+SN E +S+ F T ++ S LS V++ +S +PV+ + + +E + + + EI+ G++ +++SPF KTRS IK K + KE QSLS+ LIDKYGPHDK+KWFTDLDIS YLEWKIN DKHFALNAGIVDAIASK++DYKPPLLE+LV CD+VLCPLNIKN+HW LFVY KST+ES VM+ Sbjct: 2 WGPKIIRAINNQSHYHNACSEMLLVLRKHKLQLEYDSLNNVIDPAVISKINSLYKFIEKNSDDIKCQYN---YANNLLEQSKLFKSKNHDKELTSNAVSFTLNILRFKINLLNRGLAKCFYSKSTKTTCPYDQGIVITELIDFLTAFLLKKFKDEIPKFALPLKPETVKSA--KVGDRVPLVSSVKPKKTNPNKITLVPTGNYVSEPV---VKSNMNLYSETKVVSNKTFDARTHKHIIQILPPKSTLSPNVMEDINAS--LPVVNSEK--KIEKQCTVEQPAPAEIVRRTVGENSENDMNSPFYKTRSHIKAKGKPKECPS--DSQSLSSVLIDKYGPHDKAKWFTDLDISCYLEWKINGDKHFALNAGIVDAIASKMEDYKPPLLERLVKCDTVLCPLNIKNKHWFLFVYEKSTAESLVMD 410
BLAST of SMED30032843 vs. Planmine SMEST
Match: SMESG000062934.1 (SMESG000062934.1) HSP 1 Score: 281.952 bits (720), Expect = 3.475e-86 Identity = 134/217 (61.75%), Postives = 166/217 (76.50%), Query Frame = -3 Query: 1119 SLPVLKDKLFIKTGENSDKSINKILLRNNIDNITFSDGLFYATNKVTEFDFSTGCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPKIIERISKNSYYYTSCSEMLLVLKKHKLQLEYDSLNNIIDPGVILKINSFYKFIEKNSDNIKCQIDY 1769 +P L +KL + G+ KS N IL R + +++FSDG FY T FD S+G DILFNLGHVNF K+SKSN + +E L+I N EK+I +IFLLNCLK+E KI++ SK+ Y+ FAYNYILNCG+LHSV VDEL N+R+E+F+ WGPKII I+ S+Y+ +CSEMLLVL+KHKLQLEYDSLNN+IDP VI KINS YKFIEKNSD+IKCQ Y Sbjct: 3 GIPQLHNKLMSEDGDRLHKSANGILKRKGLSHVSFSDGSFYTAKSNTAFDLSSGGPDILFNLGHVNFRKLSKSNDELRETLQIVNPKEKLIKLIFLLNCLKIEGKISKTSKISYNNFAYNYILNCGILHSVAPVDELLNFRIENFEMWGPKIIRAINNQSHYHNACSEMLLVLRKHKLQLEYDSLNNVIDPAVISKINSLYKFIEKNSDDIKCQYHY 219 HSP 2 Score: 214.157 bits (544), Expect = 6.421e-61 Identity = 129/271 (47.60%), Postives = 173/271 (63.84%), Query Frame = -3 Query: 90 PKFALPLKPKTMLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPV-ESNQEKSCISDFLTTTRSSTVK---ASENSDLSVVL--KTAQSSTIM-------PVIETTEHNSVEIKSDISEVG-TEILNDPSGDSCAGEVSSPFCKTRSQIKFKDRVKETERVPMPQSLSATLIDKYGPHDKSKWFTDLDISSYLEWKINDDKHFALNAGIVDAIASKLDDYKPPLLEKLVMCDSVLCPLNIKNQHWVLFVYIKSTSESFVME 860 PKFALPLKP+T+ ++ +V + V +VK ++++ + D + PV ESN +F + T+ + K A + + +L K+ S +M PV+ + + +E + + + EI+ G++ +V+SPF KTRS IK K + KE QSLS+ LIDKYGPHDK+KWFTDLDIS YLEWKIN DKHFALNAGIVDAIASK++DYKPPLLE+LV CD+VLCPLNI N+HWVLFVY KST+ESFVM+ Sbjct: 238 PKFALPLKPETVKSA--KVGDRVTLVSSVKPKKTNPNKITL---VPTGDYVSEPVVESNM------NFYSETKVVSYKTFDARTHKHIIQILPPKSTLSPNVMEDINASLPVVNSEKKEKIEKQCTVEQPAPAEIVRRTVGENSENDVNSPFYKTRSHIKAKGKPKECPS--DSQSLSSVLIDKYGPHDKAKWFTDLDISCYLEWKINGDKHFALNAGIVDAIASKMEDYKPPLLERLVKCDTVLCPLNINNKHWVLFVYEKSTAESFVMD 495
BLAST of SMED30032843 vs. Planmine SMEST
Match: SMESG000047025.1 (SMESG000047025.1) HSP 1 Score: 251.521 bits (641), Expect = 1.448e-73 Identity = 158/198 (79.80%), Postives = 171/198 (86.36%), Query Frame = -3 Query: 447 AVSFISNILNFKIKLLNRGVEKCFYXXXXXXXXXSDLGAAVVEXXXXXXXXXXXXXXXXIPKFALPLKPKTMLNSCIQVDNLENSVLAVKSPSVGMVQMSVDSHLHLSDKFTVPVESNQEKSCISDFLTTTRSSTVKASENSDLSVVLKTAQSSTIMPVIETTEHNSVEIKSDISEVGTEILNDPSGDSCAGEVSSPF 1040 AVSFISNILNFKI+LLNRG EKC YSKSSKS CSSD G +VVELIDFFKEIL KK KEEI KFA+PLKPKTMLNSCIQ DNLENSVLAVK P+VGMVQMSVD H SDKFTVPVESN+EKS IS FL T RSS+VKASE SDLSVVLKTAQS++IMPVIETTEHNSVEIK DIS VGT+ LN P+ D+C E++SP Sbjct: 498 AVSFISNILNFKIRLLNRGGEKCLYSKSSKSTCSSDFGVSVVELIDFFKEILFKKLKEEILKFAMPLKPKTMLNSCIQDDNLENSVLAVKPPTVGMVQMSVDLHYFCSDKFTVPVESNREKSGISTFLMTPRSSSVKASEISDLSVVLKTAQSTSIMPVIETTEHNSVEIKGDISVVGTKTLNGPNSDNCRNELNSPL 695 The following BLAST results are available for this feature:
BLAST of SMED30032843 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032843 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032843 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032843 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032843 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032843 ID=SMED30032843|Name=SMED30032843|organism=Schmidtea mediterranea sexual|type=transcript|length=1939bpback to top protein sequence of SMED30032843-orf-1 >SMED30032843-orf-1 ID=SMED30032843-orf-1|Name=SMED30032843-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=582bp MTNTARENLVNSLPVLKDKLFIKTGENSDKSINKILLRNNIDNITFSDGLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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