Titin

Overview
NameTitin
Smed IDSMED30032833
Length (bp)2208
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Titin (SMED30032833) t-SNE clustered cells

Violin plots show distribution of expression levels for Titin (SMED30032833) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Titin (SMED30032833) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Titin (SMED30032833) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30032833

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30032833h1SMcG0011201 Contig47022uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032833h1SMcG0011201 Contig47022newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032833h1SMcG0011201 Contig5121GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032833h1SMcG0011201 Contig18125newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032833h1SMcG0011201 Contig18125uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30032833h1SMcG0011201 dd_Smed_v4_475_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032833h1SMcG0011201 dd_Smed_v4_475_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30032833h1SMcG0011201 dd_Smed_v4_475_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30032833h1SMcG0011201 dd_Smed_v4_475_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30032833h1SMcG0011201 dd_Smed_v4_475_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30032833h1SMcG0011201 dd_Smed_v6_475_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30032833h1SMcG0011201 dd_Smed_v6_475_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403])

HSP 1 Score: 108.612 bits (270), Expect = 2.233e-23
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1510 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 1954

HSP 2 Score: 72.4034 bits (176), Expect = 3.390e-12
Identity = 86/368 (23.37%), Postives = 148/368 (40.22%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVI--SDSVNKF-YGDGTINYLELTRCTE-VGQHNIRVVATNPLGQMASDCILTVN 1480
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+  S+               +NL+    S+ ++  +N Q  ++ +  + + +   Q PSF + L  + +  G   +F   +   P+  I W  N + ++   D V  F    G    L +   +E  GQ++ +  A N  G+      LTV 
Sbjct: 3191 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQVSST---------------ANLSLEGFSKFEENTSNSQWHVSLSV-SFKKEPLGQKPSFIQPLSSLRVHNGETVRFHARVSGIPKPEIQWFHNQQLILPTKDVVFHFEESTGMALMLIVDAYSEHAGQYSCK--AANSAGEATCAATLTVT 3511

HSP 3 Score: 63.1586 bits (152), Expect = 1.974e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  897 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1126

HSP 4 Score: 60.8474 bits (146), Expect = 1.219e-8
Identity = 80/355 (22.54%), Postives = 146/355 (41.13%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER--GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-------KVDNNV---------NFIE----------------NQHVQQDENEPQYPDKHESSQ---SPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAF-RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            + P F+  L + + V+  + +R  ++V+  G     ++W  + + +L        FE   G  ++ I+ +  E +G Y C A + +G+  +  AT+ VTP+A       +++  +         +F+                 N+ ++     P YP K +S +    P F K++ SN  ++ G    L   V   P+     K+ W  NG  ++  + +  VFD    +L IL+T  ED+G YTC+A N+ G       +  C    KI SK              E +   E   A  ++ E       P F K L+ +   +G  A F   +   P  ++ W   N+++ +
Sbjct: 3417 QKPSFIQPLSS-LRVHNGETVRFHARVS--GIPKPEIQWFHNQQLILPTKDVVFHFEESTGMALMLIVDAYSEHAGQYSCKAANSAGE-ATCAATLTVTPKAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEV-SNADISMGDVATLSVTVIGIPKP----KIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYG-------KTICSAYLKINSKG-------------EGHKDTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAPTVTWFKENKQLCT 3742

HSP 5 Score: 56.225 bits (134), Expect = 2.868e-7
Identity = 64/250 (25.60%), Postives = 104/250 (41.60%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDL------ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDK 1222
            V W KDGK L     F   +   G   L I    E D G YVC    + G +++N   +    ++  +      E +   Q   E     K E  +  P  SK +      ++ ++ +      ++   +PR        W K+G++++ G ++    D+G   L+I  T   D G+YTC  KN+ G   SS    I  I D +    S    S I+  KK    +QEEI+     +E+ +  +A S +K
Sbjct: 26377 VTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAI----WTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKK--AVVQEEISQKALRSEEIKMSEAKSQEK 26620

HSP 6 Score: 55.8398 bits (133), Expect = 3.634e-7
Identity = 112/505 (22.18%), Postives = 195/505 (38.61%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDN--------NVNFIENQH---VQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC-IPDEKIVSKSNL------------NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTI 1762
            A R L+     +TV E +  R S      G+   +V WL+ G+ L T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +         V   E  H   V   E +P   +K +    S  P  ++ L++  +        L  +V+       +V W+K+G+ +    + N  F   Y A          DG Y  +  NN    ES   +  C I  E   SK+NL              S IS+ KK +    E  +   R TE +  +     P    GLQD T+   + AKF +     PR + +W+ + + +      K   D    +LE+  T  ++ G +   V   N  G ++S C LT+ +I +              +K+++ K  ++TP+    + +     E   K  R+ E+K    K+ + L  ++   KV  + + K + +T   ++I
Sbjct: 26188 AARILTK-PRSMTVYEGESARFSCDTD--GEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVK-SPEPRVKSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKE---IAKLTCVVESSVLRAKEVTWYKDGKKL----KENGHFQFHYSA----------DGTYE-LKINNL--TESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTE--STVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTV--KNSAGSVSSSCKLTIKAIKDTEA-----------QKVSTQKTSEITPQKKAVVQE-----EISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSI 26648

HSP 7 Score: 53.9138 bits (128), Expect = 1.384e-6
Identity = 60/234 (25.64%), Postives = 93/234 (39.74%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   +S+  P F K L +N    +G +V  E  V      TLK  W K+GQ +SLG     + +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 25349 VTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGED-SCKAKLTVT------------------------LHPPPTDSTLRPMF-KRLLANAECQEGQSVCFEIRVSGIPPPTLK--WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYKKQEFKSKEEHERHVQKQIDKTLRMAE 25545
[Close]
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403])

HSP 1 Score: 108.612 bits (270), Expect = 2.271e-23
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1510 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 1954

HSP 2 Score: 72.0182 bits (175), Expect = 4.562e-12
Identity = 87/391 (22.25%), Postives = 154/391 (39.39%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQ------------------------EDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+  SS + ++    E I+ +    E  + ++K+F ++      +   + E Q                        ++   P F K + +  I  G+ A   + +   P+  I W  N   +   +  KF  DG  + L +  T+  + G++    +A+N  G+      L +NS
Sbjct: 3191 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLSLEAPESILHERIEQEIEM-EMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYT--CMASNDYGKTICSAYLKINS 3548

HSP 3 Score: 63.5438 bits (153), Expect = 1.829e-9
Identity = 87/351 (24.79%), Postives = 139/351 (39.60%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWL-KDGKPLLTGTRF--STVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV-------------NFIE----------------NQHVQQDENEPQYPDKHESSQ---SPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAF-RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            P  ++ L++ +T +E  P R   +V+      L V W  KD K  +  +RF   T FE  Y  L+I  +  ED G Y  VA +  GQ  S        PE+ +   +             +F+                 N+ ++     P YP K +S +    P F K++ SN  ++ G    L   V   P+     K+ W  NG  ++  + +  VFD    +L IL+T  ED+G YTC+A N+ G       +  C    KI SK              E +   E   A  ++ E       P F K L+ +   +G  A F   +   P  ++ W   N+++ +
Sbjct: 3299 PAIITPLQDTVT-SEGQPARFQCRVS---GTDLKVSWYSKDKK--IKPSRFFRMTQFEDTYQ-LEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEV-SNADISMGDVATLSVTVIGIPKP----KIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYG-------KTICSAYLKINSKG-------------EGHKDTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAPTVTWFKENKQLCT 3617

HSP 4 Score: 63.1586 bits (152), Expect = 2.007e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  897 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1126

HSP 5 Score: 56.225 bits (134), Expect = 2.703e-7
Identity = 64/250 (25.60%), Postives = 104/250 (41.60%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDL------ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDK 1222
            V W KDGK L     F   +   G   L I    E D G YVC    + G +++N   +    ++  +      E +   Q   E     K E  +  P  SK +      ++ ++ +      ++   +PR        W K+G++++ G ++    D+G   L+I  T   D G+YTC  KN+ G   SS    I  I D +    S    S I+  KK    +QEEI+     +E+ +  +A S +K
Sbjct: 26252 VTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAI----WTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKK--AVVQEEISQKALRSEEIKMSEAKSQEK 26495

HSP 6 Score: 55.8398 bits (133), Expect = 3.513e-7
Identity = 112/505 (22.18%), Postives = 195/505 (38.61%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDN--------NVNFIENQH---VQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC-IPDEKIVSKSNL------------NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTI 1762
            A R L+     +TV E +  R S      G+   +V WL+ G+ L T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +         V   E  H   V   E +P   +K +    S  P  ++ L++  +        L  +V+       +V W+K+G+ +    + N  F   Y A          DG Y  +  NN    ES   +  C I  E   SK+NL              S IS+ KK +    E  +   R TE +  +     P    GLQD T+   + AKF +     PR + +W+ + + +      K   D    +LE+  T  ++ G +   V   N  G ++S C LT+ +I +              +K+++ K  ++TP+    + +     E   K  R+ E+K    K+ + L  ++   KV  + + K + +T   ++I
Sbjct: 26063 AARILTK-PRSMTVYEGESARFSCDTD--GEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVK-SPEPRVKSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKE---IAKLTCVVESSVLRAKEVTWYKDGKKL----KENGHFQFHYSA----------DGTYE-LKINNL--TESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTE--STVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTV--KNSAGSVSSSCKLTIKAIKDTEA-----------QKVSTQKTSEITPQKKAVVQE-----EISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSI 26523

HSP 7 Score: 53.9138 bits (128), Expect = 1.372e-6
Identity = 60/234 (25.64%), Postives = 93/234 (39.74%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   +S+  P F K L +N    +G +V  E  V      TLK  W K+GQ +SLG     + +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 25224 VTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGED-SCKAKLTVT------------------------LHPPPTDSTLRPMF-KRLLANAECQEGQSVCFEIRVSGIPPPTLK--WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYKKQEFKSKEEHERHVQKQIDKTLRMAE 25420
[Close]
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403])

HSP 1 Score: 108.612 bits (270), Expect = 2.291e-23
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1510 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 1954

HSP 2 Score: 66.2402 bits (160), Expect = 2.314e-10
Identity = 90/417 (21.58%), Postives = 153/417 (36.69%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEA---------------ESSHSQITCIPDEKIVSKSNLN--ESSISQ--------------LKKFENNIQEEINDAF-----------------------RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTV 1477
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+                +SS   +      + V+ S+    ES ISQ              ++ FE+  Q  I+ A                        +    +   +AP     LQDVT+  G+ A+F+  +       + W+    ++      K   +G I +L +     V Q     +  N  G+  +  +L+V
Sbjct: 3191 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEVQALDRQSSGKDVRESTKSQAVADSSFTKEESKISQKEIKSFQGSSYEYEVQVFESVSQSSIHTAASVQDTQLCHTASLSQIAESTELSKECAKESTGEAPKIFLHLQDVTVKCGDTAQFLCVLKDDSFIDVTWTHEGAKIEESERLKQSQNGNIQFLTICNVQLVDQGLYSCIVHNDCGERTTSAVLSV 3578

HSP 3 Score: 63.1586 bits (152), Expect = 1.908e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  897 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1126

HSP 4 Score: 56.225 bits (134), Expect = 2.992e-7
Identity = 64/250 (25.60%), Postives = 104/250 (41.60%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDL------ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDK 1222
            V W KDGK L     F   +   G   L I    E D G YVC    + G +++N   +    ++  +      E +   Q   E     K E  +  P  SK +      ++ ++ +      ++   +PR        W K+G++++ G ++    D+G   L+I  T   D G+YTC  KN+ G   SS    I  I D +    S    S I+  KK    +QEEI+     +E+ +  +A S +K
Sbjct: 26444 VTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAI----WTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKK--AVVQEEISQKALRSEEIKMSEAKSQEK 26687

HSP 5 Score: 55.8398 bits (133), Expect = 3.889e-7
Identity = 112/505 (22.18%), Postives = 195/505 (38.61%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDN--------NVNFIENQH---VQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC-IPDEKIVSKSNL------------NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTI 1762
            A R L+     +TV E +  R S      G+   +V WL+ G+ L T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +         V   E  H   V   E +P   +K +    S  P  ++ L++  +        L  +V+       +V W+K+G+ +    + N  F   Y A          DG Y  +  NN    ES   +  C I  E   SK+NL              S IS+ KK +    E  +   R TE +  +     P    GLQD T+   + AKF +     PR + +W+ + + +      K   D    +LE+  T  ++ G +   V   N  G ++S C LT+ +I +              +K+++ K  ++TP+    + +     E   K  R+ E+K    K+ + L  ++   KV  + + K + +T   ++I
Sbjct: 26255 AARILTK-PRSMTVYEGESARFSCDTD--GEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVK-SPEPRVKSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKE---IAKLTCVVESSVLRAKEVTWYKDGKKL----KENGHFQFHYSA----------DGTYE-LKINNL--TESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTE--STVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTV--KNSAGSVSSSCKLTIKAIKDTEA-----------QKVSTQKTSEITPQKKAVVQE-----EISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSI 26715

HSP 6 Score: 53.9138 bits (128), Expect = 1.431e-6
Identity = 60/234 (25.64%), Postives = 93/234 (39.74%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   +S+  P F K L +N    +G +V  E  V      TLK  W K+GQ +SLG     + +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 25416 VTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGED-SCKAKLTVT------------------------LHPPPTDSTLRPMF-KRLLANAECQEGQSVCFEIRVSGIPPPTLK--WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYKKQEFKSKEEHERHVQKQIDKTLRMAE 25612

HSP 7 Score: 53.5286 bits (127), Expect = 1.695e-6
Identity = 54/211 (25.59%), Postives = 88/211 (41.71%), Query Frame = 2 
Query:  722 NQHVQQDENEPQYPDKHESSQ---SPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAF-RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            N+ ++     P YP K +S +    P F K++ SN  ++ G    L   V   P+     K+ W  NG  ++  + +  VFD    +L IL+T  ED+G YTC+A N+ G       +  C    KI SK              E +   E   A  ++ E       P F K L+ +   +G  A F   +   P  ++ W   N+++ +
Sbjct: 3624 NETLELLSESPVYPTKFDSEKEGTGPIFIKEV-SNADISMGDVATLSVTVIGIPKP----KIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYG-------KTICSAYLKINSKG-------------EGHKDTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAPTVTWFKENKQLCT 3809
[Close]
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403])

HSP 1 Score: 108.227 bits (269), Expect = 2.775e-23
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1556 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 2000

HSP 2 Score: 71.633 bits (174), Expect = 5.739e-12
Identity = 88/391 (22.51%), Postives = 149/391 (38.11%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQE----EINDAFRNTEDQEDDQA--------------------PSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY----LELTRCTEVGQHNIRVVATNPLGQMASDCILTV 1477
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+  SS + ++    E I+ +    E  +   + F     E    +  DAF ++ED +                        PSF + L    + EG    F   + + P   + W  NN  + +        +  +++    LE+ R  +    + R++A N  G   S   L V
Sbjct: 3237 EPPQVLQELQP-VTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLSLEAPESILHERIEQEIEMEMKELFSEGESEHSERDTRDAFSDSEDIDHKSMAAKRYASRISSTSSWPEYFKPSFTQKLTFKYVLEGEPVVFTCRLIACPTPEMTWFHNNRPIPTGLRRIIKAESDLHHHSSSLEIKRVQDRDSGSYRLLAINSEGSAESTASLLV 3597

HSP 3 Score: 63.929 bits (154), Expect = 1.344e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  943 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1172
[Close]
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403])

HSP 1 Score: 108.227 bits (269), Expect = 2.898e-23
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1556 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 2000

HSP 2 Score: 72.0182 bits (175), Expect = 4.095e-12
Identity = 87/391 (22.25%), Postives = 154/391 (39.39%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQ------------------------EDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+  SS + ++    E I+ +    E  + ++K+F ++      +   + E Q                        ++   P F K + +  I  G+ A   + +   P+  I W  N   +   +  KF  DG  + L +  T+  + G++    +A+N  G+      L +NS
Sbjct: 3237 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLSLEAPESILHERIEQEIEM-EMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYT--CMASNDYGKTICSAYLKINS 3594

HSP 3 Score: 63.5438 bits (153), Expect = 1.628e-9
Identity = 87/351 (24.79%), Postives = 139/351 (39.60%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWL-KDGKPLLTGTRF--STVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV-------------NFIE----------------NQHVQQDENEPQYPDKHESSQ---SPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAF-RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            P  ++ L++ +T +E  P R   +V+      L V W  KD K  +  +RF   T FE  Y  L+I  +  ED G Y  VA +  GQ  S        PE+ +   +             +F+                 N+ ++     P YP K +S +    P F K++ SN  ++ G    L   V   P+     K+ W  NG  ++  + +  VFD    +L IL+T  ED+G YTC+A N+ G       +  C    KI SK              E +   E   A  ++ E       P F K L+ +   +G  A F   +   P  ++ W   N+++ +
Sbjct: 3345 PAIITPLQDTVT-SEGQPARFQCRVS---GTDLKVSWYSKDKK--IKPSRFFRMTQFEDTYQ-LEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEV-SNADISMGDVATLSVTVIGIPKP----KIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYG-------KTICSAYLKINSKG-------------EGHKDTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAPTVTWFKENKQLCT 3663

HSP 4 Score: 63.1586 bits (152), Expect = 1.945e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  943 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1172

HSP 5 Score: 62.3882 bits (150), Expect = 3.995e-9
Identity = 65/283 (22.97%), Postives = 112/283 (39.58%), Query Frame = 2 
Query:  347 KGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSH--------------SQITCIPDEKIVSKSNLNESSISQLKKFENNIQ 1153
            K    V+  Y     + P  +      + V E+DP+ L   VA  G   L V+WLKDGK ++    FS  FE       I   +++DSG Y    ++  G +  + A +RV                    D+N P           P F+K L + +    G ++H+E  V     + +   W K+G+ +S  +++  V      +L++     ED   YTC   N  G+   S                +   IPD  +  K+ L  +   ++K F+++++
Sbjct: 6050 KNESGVERCYAFLLVQEPAQIVEKAKSVDVTEKDPMTLECVVA--GTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRVL-------------------DQNIP-----------PSFTKKL-TKMDKVLGSSIHMECKVS--GSLPISAQWFKDGKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGILTVKEPPSFLVKPGRQQAIPDSTVEFKAILKGTPPFKIKWFKDDVE 6296

HSP 6 Score: 61.2326 bits (147), Expect = 9.239e-9
Identity = 66/257 (25.68%), Postives = 99/257 (38.52%), Query Frame = 2 
Query:  305 VNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQS--------NPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKN-GQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES 1048
            +N  G +        G     S    KE + P         +TV+E + ++LS  V   G   + ++WLK G+ +    R S  F  G  VL++    + DSG YVC A + +G + +         PA    T +A VD  + F+         +EPQ     E + + + +K          GG            D    V W K   + ++ G R           L+I  T   D G+Y CVA N  GE ES
Sbjct: 9241 MNDAGLYTCKVSNDAGSALCTSSIVIKEPKKPPVFDQHLTPVTVSEGEYVQLSCHVQ--GSEPIRIQWLKAGREIKPSDRCSFSFASGTAVLELRDVAKADSGDYVCKASNVAGSDTTKSKVTIKDKPAVAPATKKAAVDGRLFFV---------SEPQSIRVVEKTTATFIAK---------VGG------------DPIPNVKWTKGKWRQLNQGGRVFIHQKGDEAKLEIRDTTKTDSGLYRCVAFNEHGEIES 9465

HSP 7 Score: 59.6918 bits (143), Expect = 2.754e-8
Identity = 65/263 (24.71%), Postives = 104/263 (39.54%), Query Frame = 2 
Query:  290 PNGNIVNAQGNHVASFG----RPKG---------HGSVQSQYESKEFEAPRFLSNLEN-EITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            PN  I +A+  H+        + +G          G+   Q E    + P F+  LE  +  +N++  + L  QV    D  ++V W KDG+ L  G  +   FE     L+I  +  +DSG YVC A +++G +  + ATV V                       EP           P F K ++ +  +  G +  L   +  +    ++V W KN + +S  +     F      LDI     ED G Y+C A N+ G
Sbjct: 4522 PNWRISDAENKHILELSNLTIQDRGVYSCKASNKFGADICQAELIIIDKPHFIKELEPVQSAINKK--VHLECQVD--EDRKVTVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAGSSSCS-ATVTV----------------------REP-----------PSFVKKVDPSYLMLPGESARLHCKL--KGSPVIQVTWFKNNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVG 4744

HSP 8 Score: 55.8398 bits (133), Expect = 3.431e-7
Identity = 64/250 (25.60%), Postives = 104/250 (41.60%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDL------ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDK 1222
            V W KDGK L     F   +   G   L I    E D G YVC    + G +++N   +    ++  +      E +   Q   E     K E  +  P  SK +      ++ ++ +      ++   +PR        W K+G++++ G ++    D+G   L+I  T   D G+YTC  KN+ G   SS    I  I D +    S    S I+  KK    +QEEI+     +E+ +  +A S +K
Sbjct: 33676 VTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAI----WTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKK--AVVQEEISQKALRSEEIKMSEAKSQEK 33919

HSP 9 Score: 55.8398 bits (133), Expect = 3.929e-7
Identity = 112/505 (22.18%), Postives = 195/505 (38.61%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDN--------NVNFIENQH---VQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC-IPDEKIVSKSNL------------NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTI 1762
            A R L+     +TV E +  R S      G+   +V WL+ G+ L T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +         V   E  H   V   E +P   +K +    S  P  ++ L++  +        L  +V+       +V W+K+G+ +    + N  F   Y A          DG Y  +  NN    ES   +  C I  E   SK+NL              S IS+ KK +    E  +   R TE +  +     P    GLQD T+   + AKF +     PR + +W+ + + +      K   D    +LE+  T  ++ G +   V   N  G ++S C LT+ +I +              +K+++ K  ++TP+    + +     E   K  R+ E+K    K+ + L  ++   KV  + + K + +T   ++I
Sbjct: 33487 AARILTK-PRSMTVYEGESARFSCDTD--GEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVK-SPEPRVKSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKE---IAKLTCVVESSVLRAKEVTWYKDGKKL----KENGHFQFHYSA----------DGTYE-LKINNL--TESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTE--STVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTV--KNSAGSVSSSCKLTIKAIKDTEA-----------QKVSTQKTSEITPQKKAVVQE-----EISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSI 33947

HSP 10 Score: 54.6842 bits (130), Expect = 7.797e-7
Identity = 74/405 (18.27%), Postives = 150/405 (37.04%), Query Frame = 2 
Query:  479 AGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEA-------------------------------ESSHS-----QITCIPDEKIVSKS--------------NLNESSISQLKKF----ENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVNSIN---NYAQDLKHI 1516
            +G   +SV W +DG  +++G +  + F      L++      D+G+Y CVA + +G ++ +                       VQ+  +  Q PD                ++ V  G ++   +++  R     KV W K  + +  G   N   +     L++    P + G Y+C+  N+ G A                               E++++      ++ I DE ++S+S               + ES+I    ++    EN   ++I +A  +       + P F + L+ V    G  A     +   P   I W   + ++ S    K      +  L + +   ++VG+++ +  A N +G +AS  +L + +      +A+ LK +
Sbjct: 7692 SGSAPISVGWFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAVLT-------------------VQEPPSFEQTPD----------------SVEVLPGMSLTFTSVI--RGTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAGSASCTTHLFVKEPATFVKRLADFSVETGSPIVLEATYTGTPPISVSWIKDEYLISQSERCSITMTEKSTILEILESTIEDYAQYSCLIENEAGQDICEALVSVL-----EPPYFIEPLEHVEAVIGEPATLQCKVDGTPEIRISWYKEHTKLRSAPAYKMQFKNNVASLVINKVDHSDVGEYSCK--ADNSVGAVASSAVLVIKARKLPPFFARKLKDV 8052

HSP 11 Score: 54.299 bits (129), Expect = 1.231e-6
Identity = 60/234 (25.64%), Postives = 93/234 (39.74%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   +S+  P F K L +N    +G +V  E  V      TLK  W K+GQ +SLG     + +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 32648 VTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGED-SCKAKLTVT------------------------LHPPPTDSTLRPMF-KRLLANAECQEGQSVCFEIRVSGIPPPTLK--WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYKKQEFKSKEEHERHVQKQIDKTLRMAE 32844

HSP 12 Score: 52.7582 bits (125), Expect = 3.666e-6
Identity = 53/225 (23.56%), Postives = 84/225 (37.33%), Query Frame = 2 
Query:  479 AGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-SNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNI 1150
            AG + +S++W KD + +    ++   F      L+I      DSG Y C A +K+G NQ S   TV+                                   + PYF +  +S   VN    V L+ +V     +T+K  W K+ + +  G+  +   D    +L++      D G Y C   N+ G A S  +     P + I   S +      Q   FE  I
Sbjct: 5626 AGSHPISIQWFKDDQEISASEKYKFSFHDNTAFLEISQLEGTDSGTYTCSATNKAGHNQCSGHLTVK-----------------------------------EPPYFVEKPQSQ-DVNPNTRVQLKALVGGTAPMTIK--WFKDNKELHSGAARSVWKDDTSTSLELFAAKATDSGTYICQLSNDVGTATSKATLFVKEPPQFIKKPSPVLVLRNGQSTTFECQI 5812

HSP 13 Score: 51.6026 bits (122), Expect = 7.461e-6
Identity = 54/224 (24.11%), Postives = 87/224 (38.84%), Query Frame = 2 
Query:  365 QSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            +S Y +KEF+           I V +E  + L ++VA  G     + W KD   L +G ++ T  +   V L IL  +  D+G Y C   ++ G   S+  + RVT                      EP           P F K +ES  ++  G A    T+   +  + + V W K+   ++        F+    +L +     + DG Y C AKN+ G
Sbjct: 5318 ESPYFTKEFKP----------IEVLKEYDVMLLAEVA--GTPPFEITWFKDNTILRSGRKYKTFIQDHLVSLQILKFVAADAGEYQCRVTNEVG---SSICSARVT--------------------LREP-----------PSFIKKIESTSSLRGGTAAFQATL---KGSLPITVTWLKDSDEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAG 5492
[Close]
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87])

HSP 1 Score: 174.096 bits (440), Expect = 1.612e-44
Identity = 131/440 (29.77%), Postives = 212/440 (48.18%), Query Frame = 2 
Query:  380 SKEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-------NNVNFIENQHVQQDENEPQYP--DKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES-SISQLKKFENNI-QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK------KVDVTPELTTALH-KPKPTAEKIVKIER 1636
            S + +AP+ +  L + +  + E D I L  +V P  D  L VEWL++G PL   +RF   FE G+V LDILY+  ED+G Y  V ++  G+ +S    + V P   +D       N  + +E+   Q  + + Q    D +  S  ++P F   L+ NI V +G     ET V P ND  LKV+W K+ + + LG RF +  D G+  LD+LY  P+D G Y CVA N  G+   S +++ C     +++ S + +   +S +KK   NI   E   A +  + Q    AP F   L+++ + E   A+F   +  +PR  + W IN  + +     K   DG ++YL +++        +  +A N  G+  S   L +   +++ Q    + P N +K    L+      + D    L  A    PKP A+K++ +ER
Sbjct: 3109 STQIDAPQVVQQLVDSVENILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQ-NIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMIS-AKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQKQ----APQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGEVVAIARNTEGETISTATLDIFQNDDFRQ--TKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVER 3537

HSP 2 Score: 169.859 bits (429), Expect = 3.059e-43
Identity = 110/388 (28.35%), Postives = 183/388 (47.16%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-KVDNN-------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRAS--------IVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            P+FLS ++      E       +++ P  D +L V WLKDG+PL    R       G V L +  +  ED+GVY CV  +  GQ QS+     V  +  ++D+        + ++++  +       + P++  S ++P F+++L   I V +   VH E  + P NDV + V+W+ NG  +    RF+ +FD GY ALD+LY YP+D G YT VA+N  GEA S+   +  +  EK++     +   + ++K+ E + ++ I +     ED+  D AP F   L D+ ++E        NIH   RA+        I W +N   +++ S  K   +     L++            V A+N LG+    C++TV        D +H
Sbjct:  468 PKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV--VGTEKVLYLEPHHPEGLERIKELEQDRRQGIAE----VEDRTCDAAPKFLNDLPDIQLNEHE------NIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 843

HSP 3 Score: 151.754 bits (382), Expect = 2.171e-37
Identity = 119/400 (29.75%), Postives = 185/400 (46.25%), Query Frame = 2 
Query:  404 FLSNLENE-ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPE----AKVDNNVNFIENQHVQQDENEPQ-YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ--EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIV--------WSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK 1555
            FL N  N  + V E +PI L  QV P  DNSL + WL+DG+ L  G RF T ++ G+V LDIL    +D+G Y C A++  GQ ++  AT+R  P+      V +  ++   Q ++  +  PQ  PD     Q P F K L   I   +G  V+LE  V P +D +L  +W  NGQ +    RF    D GY AL+ILY YPED+G YT V +N  GEA+S+   I C       + S  + +S+ ++ + E  IQ  E + D        ++ + P+  K L   TID  + ++    +H   + + +        W  N   + + S  +   D     L++            +V  N  G+  + C   V+ + +   D  H       ++M  L+
Sbjct:  891 FLKNTTNRYLQVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQEVPDL--PHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST-VDINCGHTGGNFTDS-FHPNSLHRIAELETPIQRAEPLPD--------KEKEVPTIAKPL-PATIDSVHESQ---TLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRLKSILSDTAHPESLRRIREMEQLQ 1273

HSP 4 Score: 145.591 bits (366), Expect = 1.774e-35
Identity = 85/254 (33.46%), Postives = 139/254 (54.72%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNN---------VNFIEN----QHVQQDENEPQYP---------DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIV 1090
            AP+FL++L + I +NE + I +  +  P  D ++ +EW  +G+PLLTG+R  T+ E G++ LDI  +I EDSG Y   A +  G+       + VTP  ++ ++         +N++EN      V+ ++  PQ P         D  + S + +        + V +G  +HLE  V+P ND +LK+ W ++GQS+  G RF   +D G+ +LDIL  Y +D G YTC A+N+ G+AE+  + I C P + I+
Sbjct:  739 APKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQESLGKINYLENLNKYGRVEIEDKGPQEPPTFVVPLQADLGDVSPTFFLKNTTNRYLQVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAIL 989

HSP 5 Score: 145.591 bits (366), Expect = 2.135e-35
Identity = 112/380 (29.47%), Postives = 172/380 (45.26%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLESNIT-VNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            F+ P F  NL ++    E   IRL  ++ P  D ++ V W ++G+PL   +RF       YV LDIL    EDSGVY C A S  G+  ++        ++ + + ++    + VQ+ EN  +    D      +P F   L S++  + +G  +HLE  V+P ND  L V W  NGQ ++ G RF    D GY ALDILY + +D G + CVA+N+ GEA++  +  T +P   I + S   ES     +K +  + E    A     D E D AP F + L  +   E  +A F   +   + P   I W  + + +++ +  K   D     L++            V A+N  G       LTV        D +H
Sbjct: 1417 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTI-ANFTVLPRGTIYTDSQHPES----WQKIQ--VLEAPRAAAPEKPDAEHD-APQFIEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTVTGNAVILGDTQH 1788

HSP 6 Score: 142.895 bits (359), Expect = 1.558e-34
Identity = 113/415 (27.23%), Postives = 185/415 (44.58%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN-PYKKMASLKKVDVTPELTTALHKPK 1603
            ++FEAP  ++ L+ +  + E     L +Q  P  D S+ VEWLKDG+P+    R+  V + G+ VLDIL+ ++ D+G Y     + SG+  ++ +         +    N  + + V+  E+     P+  ++     +P F + L + +   +GG  H     +P ND  L+V W+ +G+ +  GSR   +   GY  L+I  TYPED+G Y C A N  GEA +S +++TC P E I+S + L E   +  ++     + E     R      D   P F   L     + EG  A     +   + PR  I W  N + V   +  K   D     L+LT             ATN   Q  SD + T         +LK +      Y+  ++ ++ +   EL   +H+PK
Sbjct: 2603 QQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTS-TKLTCTPKEGIISATQLPERMANAGRRI---AEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATN---QHGSDEVST---------ELKVVGGGGVSYEWQSTAERKERITELEDWIHRPK 3001

HSP 7 Score: 137.117 bits (344), Expect = 1.029e-32
Identity = 118/415 (28.43%), Postives = 193/415 (46.51%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT--------PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT--IDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK-KVD---VTPELT 1582
            + P F+  L   I   E D + L +QV P  DNSL+ EWL +G+PL+   RF    + GY+ L+ILY   ED+G Y  V ++++G+ QS   TV +          ++   N+++ I          EP  PDK +  + P  +K L + I +V++   +HLE  V P +D TL+ +W  NG  +   SR+  + D G+ +LDI Y   ED G YT V  N+ G AE+S   Q+  +   K +     +  S+ ++++      E++  A + ++D    Q P F + L   T  + EG +     ++   + P   I W  +   ++  S  +   D     LE               ATN +G+  +D  L      N   D  H   E+ ++K+  ++ +VD    TPELT
Sbjct: 1020 QPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS---TVDINCGHTGGNFTDSFHPNSLHRIAELETPIQRAEP-LPDKEK--EVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRL---KSILSDTAHPESLRRIREM-----EQLQPA-KPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTDIFLECKPRRNIYLDTHH---ESSWQKIQEIENRVDEREPTPELT 1416

HSP 8 Score: 133.65 bits (335), Expect = 1.241e-31
Identity = 98/306 (32.03%), Postives = 149/306 (48.69%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH---------VQQDENE--PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQ 1231
            G  SV+   E    EAP+F   L  +I V E + +   +++ PA D  ++VEW  +G PL    RF  +F+ GYV LD+LY+  +DSG Y  VA+++ G+ +SN   V  T        V ++E  H         ++QD  +   +  D+     +P F  DL  +I +N+   +H++    P ND T+ ++W  NG+ +  GSR   + + G+ ALDI     ED G Y+  A N  GEA      IT  P  +I+S +   ES    L K   N  E +N   R   ED+   + P+F   LQ
Sbjct:  590 GPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELVVGT------EKVLYLEPHHPEGLERIKELEQDRRQGIAEVEDR-TCDAAPKFLNDL-PDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEA-IRQCVITVTPAGQILSDTQHQES----LGKI--NYLENLNKYGRVEIEDKGPQEPPTFVVPLQ 880

HSP 9 Score: 132.109 bits (331), Expect = 3.855e-31
Identity = 115/427 (26.93%), Postives = 194/427 (45.43%), Query Frame = 2 
Query:  434 VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDEN--EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHP----RASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVD-VTPELTTALHKPKPTAEKIVKIERNAEVKARHTKT 1669
            + E   + +   V P  D SL +EW  DG+PL+ G+R  T+ + GYV L+ L+   ED+G Y C A +  G+     AT  +  E K   N+ +++  H      +Q+ EN  + + P    + Q P F+++L       +G ++ LE  + P ND T++V W +NGQ +   SRF    +  Y  LDIL  Y ED GVYTC A +  GEA +S + + C   + ++  + ++++S  ++++ EN  + E  DA      + +  AP F   L     +        +     P    + ++ W  N + + +   ++F       Y+ L       Q       VA N LG+  +    TV        D +H  PE+ ++K+  L+      PE   A H      E +  I+R  E ++ H +T
Sbjct: 1300 LKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGE-----ATTDIFLECKPRRNI-YLDTHHESSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT-VKCAAGKSLLLDT-MHDASWKRVQEIENREKLEAVDA------EPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANG--HRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH--PES-WQKIQVLEAPRAAAPEKPDAEHDAPQFIEPLDSIDR-MEFQSAHFQT 1706

HSP 10 Score: 128.642 bits (322), Expect = 4.982e-30
Identity = 109/381 (28.61%), Postives = 164/381 (43.04%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-----DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHS--HPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            E P FL+ LE+   V E  P+ L S   PA D  L V W K+G+PL       T  E G+  LDI  + E+ +GVY     +  G+  S  A++RV     +  N    E+    Q      E EP+ P        P  +  ++     N+G  VH E ++ P ND  L+V W +NG  ++ GS++    D G C LD+ YTYPED+GVY     N  GEA SS + + C   + I+      ES   I +++  +  ++E          D E    P F + L    ++ E   A F   +     P  +I W +N E + + S  K   D     +++ +         + VA N  G+  S   + V       QD
Sbjct: 2211 EKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVST-ASIRVAGTGPILGNTRHEESWKRIQILEAPKEAEPEAPAP--VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQKLEE---------ADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEAVSTATIEVQGKEVILQD 2577

HSP 11 Score: 128.257 bits (321), Expect = 5.434e-30
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKK-FENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            ++ AP F   L +   +NE D       + P GD +L V+W  +G P+    R S   E G   L I + I  D+G Y CVA +  G   S  +  ++  E+    +   +  Q V   EN                         + +G ++HLE  V P ND  L V+W +NG  +   SRF   F+ G+ +LDILY YPED+G Y  V +N+ GEA S  ++IT +P   +   S  + +    L+  F+ + Q ++     +  ++ D +AP F   LQ++ + EG   +F   +   + P   + W  + + V+     +   D     L+L             VATN  GQ      L     ++   D
Sbjct: 3010 DYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKG---SATSVGKIAVESSTQIDAPQVVQQLVDSVEN-------------------------ILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITD 3356

HSP 12 Score: 121.324 bits (303), Expect = 8.458e-28
Identity = 105/389 (26.99%), Postives = 170/389 (43.70%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE---SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTID-----EGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +E+E P+++   EN   V E   + L   V P+GD  L +EWL +G PL    RF   +E G  +L I++ +  DSGVY C A +  G+  ++        E  + ++ + +  + +Q+ E  P+  ++ E     + P F   LES   V +G  +HLE+   P  D  LKV W KNGQ +          + G+  LDI     + +GVYT    N+ GEA S+ S I       I+  +  +E S  +++  E              E + +  AP +D       ID     EG+   F  +I   + PR  + W  N   +   S      D  I  L++  T   + G + +R+   NP G+  S   L  +  +    D++H
Sbjct: 2076 REYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEKILYDSQHPVSWERIQELE-APKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVSTAS-IRVAGTGPILGNTR-HEESWKRIQILEA-----------PKEAEPEAPAPVYDHPAITTQIDDKECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRI--WNPEGEAVSSATLKCHGKDAILGDVQH 2448

HSP 13 Score: 99.7525 bits (247), Expect = 4.407e-21
Identity = 79/271 (29.15%), Postives = 118/271 (43.54%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-----------------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +GN  ++  +H     + K   +    Y   +  AP F + L+N I V E +  R  +QVAP  D  L VEW KD KP+L G RF +  + G+  LD+LY++ +D+G Y CVA ++ GQ                 +   P  +RV+   K + N+ + E     Q + +          Q+P F+  L +  +T NQ           PR  VT    W  NG     G RF   FD G   L +  +   D G    +A+N  GE  S+
Sbjct: 3221 HGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQN-IGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQK----------QAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVT----WFINGNQCLHGHRFKLNFD-GLHYLTVSKSRISDAGEVVAIARNTEGETIST 3475

HSP 14 Score: 98.5969 bits (244), Expect = 9.279e-21
Identity = 68/228 (29.82%), Postives = 104/228 (45.61%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
             + P F S+L ++  V E   I L +++ P  D  L V WL DG  L +  ++      G+  LDI  +   DSG+Y C A +K G++               +N    I   H  QD              +P F   ++      + G    E  + P ND TL+V W K+GQ +   +R     + G  +L +  TYPED GVYTCV  N+ G+A+SS +++T +
Sbjct:  364 LDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGES---------------ENQATIIIFAHSSQD-------------LTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSS-AELTTV 561

HSP 15 Score: 94.7449 bits (234), Expect = 1.712e-19
Identity = 93/414 (22.46%), Postives = 177/414 (42.75%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS---QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKP 1606
            P+F++ L +   V E  P    +Q  P  D   SV+W  +G PL   +R     + G V LD+ Y++ ED+G Y  V K+  G+++++   +  T  A +  +    ++    Q+   P+ P         + P F + L+S   + +G    LE  + P ND  L+V W  N Q +   +  +   D G  +L I   Y    GVY+C A N+ G A +S + +     E ++    L+ S  + L+K +    E I+   R    + + + P + +G ++   + EG       ++     P   + W +N   +   + N+F     +G+  +  + +    + G +  R  A N  G+ ++   +T         D +H      ++++  L+   +  E+   + K KP
Sbjct: 1816 PKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAEPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLL----LDTSHPASLQKIQE--LEAIDKYARLDAPEREYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPL--RNANRFRQEYEFGNAILTIVHVL-PHDSGVYTCR--AWNTQGEASTSATVTTAGYEKILYDSQHPV---SWERIQELEAPKIVEEIEEIVQKEKP 2213

HSP 16 Score: 94.3597 bits (233), Expect = 1.997e-19
Identity = 79/271 (29.15%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-PEAKVDNNVNFIENQHVQQDENEPQY-----PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFEN-NIQEEIND 1168
            E   +E P F+  L++   + E     L +++ P  D +L V+W  + +PL+     ST  + G V L I       +GVY C A + SG N    A V V   E  + +  +    Q +Q+ E   +Y     P++    + P + +  E+   V +G  V L  +V+P  D  L+++W  NG  +   +RF   ++ G   L I++  P D GVYTC A N  GEA +S + +T    EKI+  S  +  S  ++++ E   I EEI +
Sbjct: 1941 EGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSG-NAVTSANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPERE--YEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTS-ATVTTAGYEKILYDSQ-HPVSWERIQELEAPKIVEEIEE 2206

HSP 17 Score: 92.0485 bits (227), Expect = 1.012e-18
Identity = 84/364 (23.08%), Postives = 146/364 (40.11%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            +AP F + L N++ + +  P   S Q+   G+   +++W KDG+PL    RF  V E G   L     I  D+G+Y  VAK+  G             EA+    +N    +  +  E  P+Y       ++P F+  ++  I V++G             D T++  W++N + +     +     +G   L I     ED   Y   A N  G+A SS + +   P    ++KS ++                    A   + D+    +P F   L D++  +G+  KF   +  +P  ++ W    + + + +  K   DG    LEL R T       ++V  N  G   S   LT+++
Sbjct: 3899 KAPDFSARL-NDLRIQQNGPAEFSCQIG--GEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVG-------------EARCKARLNVNLQKTGKGAEEGPRY-------EAPRFTSQIQP-IVVDEGKGAQFSAQFSGFPDPTIR--WYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKA-SSVANLVLTPRSGRIAKSTISRG----------------GSASYQSSDKAAADSPHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLSA 4219

HSP 18 Score: 84.3445 bits (207), Expect = 2.583e-16
Identity = 98/379 (25.86%), Postives = 154/379 (40.63%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +P+F  +L+++  V E    RL  +  P  D +L + WL +GK +L  +R  T  + G   L+I      D G Y  VA +  G+        RV+    V  + +FI+ Q          Y  K   + +      P F  DL S   V +G  +HLET + P ND  L+V W  +G  +    ++      G+ +LDI  T   D G+Y+C A N  GE+E+  + I         S  +L    +SQ++ F  + ++E+  +F     Q     P  D  L+   + +G            P A+ +    N  V+S S++  Y +    Y     C     H          GQ  S   LT   I+    D KH
Sbjct:  238 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGE-------ARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATIIIF-----AHSSQDLTPKFLSQIQPF--HCEQELGRSFFEARIQ-----PINDPTLRVSWLKDGQPL---------PNANRIQIFQNFGVVSLSLHPTYPEDAGVYT----CVLFNSH----------GQAQSSAELTTVWIDTLQLDSKH 572

HSP 19 Score: 50.447 bits (119), Expect = 5.548e-6
Identity = 36/99 (36.36%), Postives = 44/99 (44.44%), Query Frame = 2 
Query:  755 QYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            Q  D   S  SP F   L+S   V +G +  LE    P  D  L++ W  NG+ +   SR     D G  AL+I      D G YT VA N  GEA  S
Sbjct:  228 QLQDVSASKSSPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVS 325
[Close]
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87])

HSP 1 Score: 174.096 bits (440), Expect = 1.648e-44
Identity = 131/440 (29.77%), Postives = 212/440 (48.18%), Query Frame = 2 
Query:  380 SKEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-------NNVNFIENQHVQQDENEPQYP--DKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES-SISQLKKFENNI-QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK------KVDVTPELTTALH-KPKPTAEKIVKIER 1636
            S + +AP+ +  L + +  + E D I L  +V P  D  L VEWL++G PL   +RF   FE G+V LDILY+  ED+G Y  V ++  G+ +S    + V P   +D       N  + +E+   Q  + + Q    D +  S  ++P F   L+ NI V +G     ET V P ND  LKV+W K+ + + LG RF +  D G+  LD+LY  P+D G Y CVA N  G+   S +++ C     +++ S + +   +S +KK   NI   E   A +  + Q    AP F   L+++ + E   A+F   +  +PR  + W IN  + +     K   DG ++YL +++        +  +A N  G+  S   L +   +++ Q    + P N +K    L+      + D    L  A    PKP A+K++ +ER
Sbjct: 3815 STQIDAPQVVQQLVDSVENILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQ-NIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMIS-AKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQKQ----APQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGEVVAIARNTEGETISTATLDIFQNDDFRQ--TKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVER 4243

HSP 2 Score: 170.244 bits (430), Expect = 2.937e-43
Identity = 112/400 (28.00%), Postives = 189/400 (47.25%), Query Frame = 2 
Query:  362 VQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-KVDNN-------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRAS--------IVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +Q  + S++   P+FLS ++      E       +++ P  D +L V WLKDG+PL    R       G V L +  +  ED+GVY CV  +  GQ QS+     V  +  ++D+        + ++++  +       + P++  S ++P F+++L   I V +   VH E  + P NDV + V+W+ NG  +    RF+ +FD GY ALD+LY YP+D G YT VA+N  GEA S+   +  +  EK++     +   + ++K+ E + ++ I +     ED+  D AP F   L D+ ++E        NIH   RA+        I W +N   +++ S  K   +     L++            V A+N LG+    C++TV        D +H
Sbjct: 1179 IQFAHSSQDL-TPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV--VGTEKVLYLEPHHPEGLERIKELEQDRRQGIAE----VEDRTCDAAPKFLNDLPDIQLNEHE------NIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 1565

HSP 3 Score: 151.754 bits (382), Expect = 2.177e-37
Identity = 119/404 (29.46%), Postives = 188/404 (46.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEIT-VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPE----AKVDNNVNFIENQHVQQDENEPQ-YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ--EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIV--------WSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK 1555
            E P F+  L+ ++  V E +PI L  QV P  DNSL + WL+DG+ L  G RF T ++ G+V LDIL    +D+G Y C A++  GQ ++  AT+R  P+      V +  ++   Q ++  +  PQ  PD     Q P F K L   I   +G  V+LE  V P +D +L  +W  NGQ +    RF    D GY AL+ILY YPED+G YT V +N  GEA+S+   I C       + S  + +S+ ++ + E  IQ  E + D        ++ + P+  K L   TID  + ++    +H   + + +        W  N   + + S  +   D     L++            +V  N  G+  + C   V+ + +   D  H       ++M  L+
Sbjct: 1593 EPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQEVPDL--PHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST-VDINCGHTGGNFTDS-FHPNSLHRIAELETPIQRAEPLPD--------KEKEVPTIAKPL-PATIDSVHESQ---TLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRLKSILSDTAHPESLRRIREMEQLQ 1979

HSP 4 Score: 148.673 bits (374), Expect = 1.982e-36
Identity = 116/420 (27.62%), Postives = 202/420 (48.10%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-------QHVQQDEN-EPQYP-DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMAS---LKKVDVTPELT 1582
            KE E P     L   I +V+E   + L +QV P  DN+L  EWL +G PL   +R+  + + G+V LDI Y I EDSG Y  V  + +G+ ++       + E +VD   + + +       + +++ E  +P  P D   ++Q P F++ L      + +G +VH++ +V P ND +L+++W  +G+ +  GSR   + D GY  L+ L+ +PED G YTC A N  GEA ++   + C P   I   ++ +ESS  ++++ EN + E      R    +   Q P+F + L D     EG + +    +I ++  P   + W+ N + +   S  +F      +Y+ L      G+ +      A +  G+ A+ C +   +  +   D  H A     +++ +   L+ VD  PE T
Sbjct: 1855 KEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-------SCEFQVDRLKSILSDTAHPESLRRIREMEQLQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE------REPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVND-PTMRVTWTRNGQPLPEAS--RFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKT 2256

HSP 5 Score: 145.591 bits (366), Expect = 2.159e-35
Identity = 112/380 (29.47%), Postives = 172/380 (45.26%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            F+ P F  NL ++    E   IRL  ++ P  D ++ V W ++G+PL   +RF       YV LDIL    EDSGVY C A S  G+  ++        ++ + + ++    + VQ+ EN  +    D      +P F   L S++  + +G  +HLE  V+P ND  L V W  NGQ ++ G RF    D GY ALDILY + +D G + CVA+N+ GEA++  +  T +P   I + S   ES     +K +  + E    A     D E D AP F + L  +   E  +A F   +   + P   I W  + + +++ +  K   D     L++            V A+N  G       LTV        D +H
Sbjct: 2123 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTI-ANFTVLPRGTIYTDSQHPES----WQKIQ--VLEAPRAAAPEKPDAEHD-APQFIEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTVTGNAVILGDTQH 2494

HSP 6 Score: 142.895 bits (359), Expect = 1.690e-34
Identity = 114/416 (27.40%), Postives = 185/416 (44.47%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV---DNNVNFIENQHVQQD--ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN-PYKKMASLKKVDVTPELTTALHKPK 1603
            ++FEAP  ++ L+ +  + E     L +Q  P  D S+ VEWLKDG+P+    R+  V + G+ VLDIL+ ++ D+G Y     + SG+  S   +  V+  + +     N    +   + +D     P+  ++     +P F + L + +   +GG  H     +P ND  L+V W+ +G+ +  GSR   +   GY  L+I  TYPED+G Y C A N  GEA +S +++TC P E I+S + L E   +  ++     + E     R      D   P F   L     + EG  A     +   + PR  I W  N + V   +  K   D     L+LT             ATN   Q  SD + T         +LK +      Y+  ++ ++ +   EL   +H+PK
Sbjct: 3309 QQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGE-ASTSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTS-TKLTCTPKEGIISATQLPERMANAGRRI---AEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATN---QHGSDEVST---------ELKVVGGGGVSYEWQSTAERKERITELEDWIHRPK 3707

HSP 7 Score: 133.65 bits (335), Expect = 1.298e-31
Identity = 98/306 (32.03%), Postives = 149/306 (48.69%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH---------VQQDENE--PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQ 1231
            G  SV+   E    EAP+F   L  +I V E + +   +++ PA D  ++VEW  +G PL    RF  +F+ GYV LD+LY+  +DSG Y  VA+++ G+ +SN   V  T        V ++E  H         ++QD  +   +  D+     +P F  DL  +I +N+   +H++    P ND T+ ++W  NG+ +  GSR   + + G+ ALDI     ED G Y+  A N  GEA      IT  P  +I+S +   ES    L K   N  E +N   R   ED+   + P+F   LQ
Sbjct: 1312 GPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELVVGT------EKVLYLEPHHPEGLERIKELEQDRRQGIAEVEDR-TCDAAPKFLNDL-PDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEA-IRQCVITVTPAGQILSDTQHQES----LGKI--NYLENLNKYGRVEIEDKGPQEPPTFVVPLQ 1602

HSP 8 Score: 128.642 bits (322), Expect = 4.613e-30
Identity = 109/381 (28.61%), Postives = 164/381 (43.04%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-----DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHS--HPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            E P FL+ LE+   V E  P+ L S   PA D  L V W K+G+PL       T  E G+  LDI  + E+ +GVY     +  G+  S  A++RV     +  N    E+    Q      E EP+ P        P  +  ++     N+G  VH E ++ P ND  L+V W +NG  ++ GS++    D G C LD+ YTYPED+GVY     N  GEA SS + + C   + I+      ES   I +++  +  ++E          D E    P F + L    ++ E   A F   +     P  +I W +N E + + S  K   D     +++ +         + VA N  G+  S   + V       QD
Sbjct: 2917 EKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVST-ASIRVAGTGPILGNTRHEESWKRIQILEAPKEAEPEAPAP--VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQKLEE---------ADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEAVSTATIEVQGKEVILQD 3283

HSP 9 Score: 128.257 bits (321), Expect = 5.394e-30
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKK-FENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            ++ AP F   L +   +NE D       + P GD +L V+W  +G P+    R S   E G   L I + I  D+G Y CVA +  G   S  +  ++  E+    +   +  Q V   EN                         + +G ++HLE  V P ND  L V+W +NG  +   SRF   F+ G+ +LDILY YPED+G Y  V +N+ GEA S  ++IT +P   +   S  + +    L+  F+ + Q ++     +  ++ D +AP F   LQ++ + EG   +F   +   + P   + W  + + V+     +   D     L+L             VATN  GQ      L     ++   D
Sbjct: 3716 DYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKG---SATSVGKIAVESSTQIDAPQVVQQLVDSVEN-------------------------ILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITD 4062

HSP 10 Score: 121.324 bits (303), Expect = 8.535e-28
Identity = 105/389 (26.99%), Postives = 170/389 (43.70%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE---SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTID-----EGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +E+E P+++   EN   V E   + L   V P+GD  L +EWL +G PL    RF   +E G  +L I++ +  DSGVY C A +  G+  ++        E  + ++ + +  + +Q+ E  P+  ++ E     + P F   LES   V +G  +HLE+   P  D  LKV W KNGQ +          + G+  LDI     + +GVYT    N+ GEA S+ S I       I+  +  +E S  +++  E              E + +  AP +D       ID     EG+   F  +I   + PR  + W  N   +   S      D  I  L++  T   + G + +R+   NP G+  S   L  +  +    D++H
Sbjct: 2782 REYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEKILYDSQHPVSWERIQELE-APKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVSTAS-IRVAGTGPILGNTR-HEESWKRIQILEA-----------PKEAEPEAPAPVYDHPAITTQIDDKECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRI--WNPEGEAVSSATLKCHGKDAILGDVQH 3154

HSP 11 Score: 99.7525 bits (247), Expect = 4.472e-21
Identity = 79/271 (29.15%), Postives = 118/271 (43.54%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-----------------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +GN  ++  +H     + K   +    Y   +  AP F + L+N I V E +  R  +QVAP  D  L VEW KD KP+L G RF +  + G+  LD+LY++ +D+G Y CVA ++ GQ                 +   P  +RV+   K + N+ + E     Q + +          Q+P F+  L +  +T NQ           PR  VT    W  NG     G RF   FD G   L +  +   D G    +A+N  GE  S+
Sbjct: 3927 HGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQN-IGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQK----------QAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVT----WFINGNQCLHGHRFKLNFD-GLHYLTVSKSRISDAGEVVAIARNTEGETIST 4181

HSP 12 Score: 94.3597 bits (233), Expect = 2.131e-19
Identity = 93/414 (22.46%), Postives = 177/414 (42.75%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS---QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKP 1606
            P+F++ L +   V E  P    +Q  P  D   SV+W  +G PL   +R     + G V LD+ Y++ ED+G Y  V K+  G+++++   +  T  A +  +    ++    Q+   P+ P         + P F + L+S   + +G    LE  + P ND  L+V W  N Q +   +  +   D G  +L I   Y    GVY+C A N+ G A +S + +     E ++    L+ S  + L+K +    E I+   R    + + + P + +G ++   + EG       ++     P   + W +N   +   + N+F     +G+  +  + +    + G +  R  A N  G+ ++   +T         D +H      ++++  L+   +  E+   + K KP
Sbjct: 2522 PKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAEPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLL----LDTSHPASLQKIQE--LEAIDKYARLDAPEREYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPL--RNANRFRQEYEFGNAILTIVHVL-PHDSGVYTCR--AWNTQGEASTSATVTTAGYEKILYDSQHPV---SWERIQELEAPKIVEEIEEIVQKEKP 2919

HSP 13 Score: 94.3597 bits (233), Expect = 2.341e-19
Identity = 79/271 (29.15%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-PEAKVDNNVNFIENQHVQQDENEPQY-----PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFEN-NIQEEIND 1168
            E   +E P F+  L++   + E     L +++ P  D +L V+W  + +PL+     ST  + G V L I       +GVY C A + SG N    A V V   E  + +  +    Q +Q+ E   +Y     P++    + P + +  E+   V +G  V L  +V+P  D  L+++W  NG  +   +RF   ++ G   L I++  P D GVYTC A N  GEA +S + +T    EKI+  S  +  S  ++++ E   I EEI +
Sbjct: 2647 EGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSG-NAVTSANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPERE--YEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTS-ATVTTAGYEKILYDSQ-HPVSWERIQELEAPKIVEEIEE 2912

HSP 14 Score: 92.0485 bits (227), Expect = 1.175e-18
Identity = 84/363 (23.14%), Postives = 145/363 (39.94%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            +AP F + L N++ + +  P   S Q+   G+   +++W KDG+PL    RF  V E G   L     I  D+G+Y  VAK+  G             EA+    +N    +  +  E  P+Y       ++P F+  ++  I V++G             D T++  W++N + +     +     +G   L I     ED   Y   A N  G+A SS + +   P    ++KS ++                    A   + D+    +P F   L D++  +G+  KF   +  +P  ++ W    + + + +  K   DG    LEL R T       ++V  N  G   S   LT++
Sbjct: 4569 KAPDFSARL-NDLRIQQNGPAEFSCQIG--GEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVG-------------EARCKARLNVNLQKTGKGAEEGPRY-------EAPRFTSQIQP-IVVDEGKGAQFSAQFSGFPDPTIR--WYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKA-SSVANLVLTPRSGRIAKSTISRG----------------GSASYQSSDKAAADSPHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4888

HSP 15 Score: 87.4261 bits (215), Expect = 2.689e-17
Identity = 102/385 (26.49%), Postives = 160/385 (41.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFI---INIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +P+F  +L+++  V E    RL  +  P  D +L + WL +GK +L  +R  T  + G   L+I      D G Y  VA +  G+        RV+    V  + +FI+ Q          Y  K   + +      P F  DL S   V +G  +HLET + P ND  L+V W  +G  +    ++      G+ +LDI  T   D G+Y+C A N  GE+E   +Q T I    IV KS+        L++FE + Q ++ D           D  P F   +Q    ++     F    I   + P   + W  + + +  ++ +  F   G ++  L  T   + G +    V  N  GQ  S   LT   I+    D KH
Sbjct:  936 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGE-------ARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESE---NQATII----IVPKSD--------LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYT--CVLFNSHGQAQSSAELTTVWIDTLQLDSKH 1294

HSP 16 Score: 72.0182 bits (175), Expect = 1.418e-12
Identity = 71/250 (28.40%), Postives = 106/250 (42.40%), Query Frame = 2 
Query:  356 GSVQSQY-----ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG-------------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            GS QS++     ++K  EAP F    ++  T+ + D ++L  + A  G   +S +W KD +P+ +G  ++   +     L I  +  ++ G Y C A +K G             Q  + PA   +    KVD     +E      +    Q  D   S  SP F   L+S   V +G +  LE    P  D  L++ W  NG+ +   SR     D G  AL+I      D G YT VA N  GEA  S
Sbjct:  787 GSFQSRFSLNVLQAKSPEAPEFTGKFQS-TTLYDGDSVKLYCKAAGEG---VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTTTLKGRVVVNSRQKFNGPAHREMITLRKVDK----VERSRTPVN----QLQDVSASKSSPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVS 1023
[Close]
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87])

HSP 1 Score: 173.711 bits (439), Expect = 1.737e-44
Identity = 131/440 (29.77%), Postives = 212/440 (48.18%), Query Frame = 2 
Query:  380 SKEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-------NNVNFIENQHVQQDENEPQYP--DKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES-SISQLKKFENNI-QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK------KVDVTPELTTALH-KPKPTAEKIVKIER 1636
            S + +AP+ +  L + +  + E D I L  +V P  D  L VEWL++G PL   +RF   FE G+V LDILY+  ED+G Y  V ++  G+ +S    + V P   +D       N  + +E+   Q  + + Q    D +  S  ++P F   L+ NI V +G     ET V P ND  LKV+W K+ + + LG RF +  D G+  LD+LY  P+D G Y CVA N  G+   S +++ C     +++ S + +   +S +KK   NI   E   A +  + Q    AP F   L+++ + E   A+F   +  +PR  + W IN  + +     K   DG ++YL +++        +  +A N  G+  S   L +   +++ Q    + P N +K    L+      + D    L  A    PKP A+K++ +ER
Sbjct: 3815 STQIDAPQVVQQLVDSVENILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQ-NIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMIS-AKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQKQ----APQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGEVVAIARNTEGETISTATLDIFQNDDFRQ--TKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVER 4243

HSP 2 Score: 169.859 bits (429), Expect = 3.041e-43
Identity = 112/400 (28.00%), Postives = 189/400 (47.25%), Query Frame = 2 
Query:  362 VQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-KVDNN-------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRAS--------IVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +Q  + S++   P+FLS ++      E       +++ P  D +L V WLKDG+PL    R       G V L +  +  ED+GVY CV  +  GQ QS+     V  +  ++D+        + ++++  +       + P++  S ++P F+++L   I V +   VH E  + P NDV + V+W+ NG  +    RF+ +FD GY ALD+LY YP+D G YT VA+N  GEA S+   +  +  EK++     +   + ++K+ E + ++ I +     ED+  D AP F   L D+ ++E        NIH   RA+        I W +N   +++ S  K   +     L++            V A+N LG+    C++TV        D +H
Sbjct: 1179 IQFAHSSQDL-TPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV--VGTEKVLYLEPHHPEGLERIKELEQDRRQGIAE----VEDRTCDAAPKFLNDLPDIQLNEHE------NIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 1565

HSP 3 Score: 151.754 bits (382), Expect = 2.233e-37
Identity = 119/404 (29.46%), Postives = 188/404 (46.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEIT-VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPE----AKVDNNVNFIENQHVQQDENEPQ-YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ--EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIV--------WSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK 1555
            E P F+  L+ ++  V E +PI L  QV P  DNSL + WL+DG+ L  G RF T ++ G+V LDIL    +D+G Y C A++  GQ ++  AT+R  P+      V +  ++   Q ++  +  PQ  PD     Q P F K L   I   +G  V+LE  V P +D +L  +W  NGQ +    RF    D GY AL+ILY YPED+G YT V +N  GEA+S+   I C       + S  + +S+ ++ + E  IQ  E + D        ++ + P+  K L   TID  + ++    +H   + + +        W  N   + + S  +   D     L++            +V  N  G+  + C   V+ + +   D  H       ++M  L+
Sbjct: 1593 EPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQEVPDL--PHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST-VDINCGHTGGNFTDS-FHPNSLHRIAELETPIQRAEPLPD--------KEKEVPTIAKPL-PATIDSVHESQ---TLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRLKSILSDTAHPESLRRIREMEQLQ 1979

HSP 4 Score: 148.673 bits (374), Expect = 1.945e-36
Identity = 116/420 (27.62%), Postives = 202/420 (48.10%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-------QHVQQDEN-EPQYP-DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMAS---LKKVDVTPELT 1582
            KE E P     L   I +V+E   + L +QV P  DN+L  EWL +G PL   +R+  + + G+V LDI Y I EDSG Y  V  + +G+ ++       + E +VD   + + +       + +++ E  +P  P D   ++Q P F++ L      + +G +VH++ +V P ND +L+++W  +G+ +  GSR   + D GY  L+ L+ +PED G YTC A N  GEA ++   + C P   I   ++ +ESS  ++++ EN + E      R    +   Q P+F + L D     EG + +    +I ++  P   + W+ N + +   S  +F      +Y+ L      G+ +      A +  G+ A+ C +   +  +   D  H A     +++ +   L+ VD  PE T
Sbjct: 1855 KEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-------SCEFQVDRLKSILSDTAHPESLRRIREMEQLQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE------REPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVND-PTMRVTWTRNGQPLPEAS--RFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKT 2256

HSP 5 Score: 145.591 bits (366), Expect = 2.157e-35
Identity = 114/382 (29.84%), Postives = 175/382 (45.81%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            F+ P F  NL ++    E   IRL  ++ P  D ++ V W ++G+PL   +RF       YV LDIL    EDSGVY C A S  G+  ++        ++ + + ++    + VQ+ EN  +    D      +P F   L S++  + +G  +HLE  V+P ND  L V W  NGQ ++ G RF    D GY ALDILY + +D G + CVA+N+ GEA++  +  T +P   I + S   ES     +K +  + E    A     D E D AP F + L  +   E  +A F   +   + P   I W  + + +++   N+F       Y+ L     V + +    V A+N  G       LTV        D +H
Sbjct: 2123 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTI-ANFTVLPRGTIYTDSQHPES----WQKIQ--VLEAPRAAAPEKPDAEHD-APQFIEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNS--NRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTVTGNAVILGDTQH 2494

HSP 6 Score: 142.895 bits (359), Expect = 1.588e-34
Identity = 114/416 (27.40%), Postives = 185/416 (44.47%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV---DNNVNFIENQHVQQD--ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN-PYKKMASLKKVDVTPELTTALHKPK 1603
            ++FEAP  ++ L+ +  + E     L +Q  P  D S+ VEWLKDG+P+    R+  V + G+ VLDIL+ ++ D+G Y     + SG+  S   +  V+  + +     N    +   + +D     P+  ++     +P F + L + +   +GG  H     +P ND  L+V W+ +G+ +  GSR   +   GY  L+I  TYPED+G Y C A N  GEA +S +++TC P E I+S + L E   +  ++     + E     R      D   P F   L     + EG  A     +   + PR  I W  N + V   +  K   D     L+LT             ATN   Q  SD + T         +LK +      Y+  ++ ++ +   EL   +H+PK
Sbjct: 3309 QQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGE-ASTSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTS-TKLTCTPKEGIISATQLPERMANAGRRI---AEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATN---QHGSDEVST---------ELKVVGGGGVSYEWQSTAERKERITELEDWIHRPK 3707

HSP 7 Score: 133.265 bits (334), Expect = 1.465e-31
Identity = 98/306 (32.03%), Postives = 149/306 (48.69%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH---------VQQDENE--PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQ 1231
            G  SV+   E    EAP+F   L  +I V E + +   +++ PA D  ++VEW  +G PL    RF  +F+ GYV LD+LY+  +DSG Y  VA+++ G+ +SN   V  T        V ++E  H         ++QD  +   +  D+     +P F  DL  +I +N+   +H++    P ND T+ ++W  NG+ +  GSR   + + G+ ALDI     ED G Y+  A N  GEA      IT  P  +I+S +   ES    L K   N  E +N   R   ED+   + P+F   LQ
Sbjct: 1312 GPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELVVGT------EKVLYLEPHHPEGLERIKELEQDRRQGIAEVEDR-TCDAAPKFLNDL-PDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEA-IRQCVITVTPAGQILSDTQHQES----LGKI--NYLENLNKYGRVEIEDKGPQEPPTFVVPLQ 1602

HSP 8 Score: 128.642 bits (322), Expect = 4.689e-30
Identity = 109/381 (28.61%), Postives = 164/381 (43.04%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-----DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHS--HPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            E P FL+ LE+   V E  P+ L S   PA D  L V W K+G+PL       T  E G+  LDI  + E+ +GVY     +  G+  S  A++RV     +  N    E+    Q      E EP+ P        P  +  ++     N+G  VH E ++ P ND  L+V W +NG  ++ GS++    D G C LD+ YTYPED+GVY     N  GEA SS + + C   + I+      ES   I +++  +  ++E          D E    P F + L    ++ E   A F   +     P  +I W +N E + + S  K   D     +++ +         + VA N  G+  S   + V       QD
Sbjct: 2917 EKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVST-ASIRVAGTGPILGNTRHEESWKRIQILEAPKEAEPEAPAP--VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQKLEE---------ADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEAVSTATIEVQGKEVILQD 3283

HSP 9 Score: 128.257 bits (321), Expect = 5.878e-30
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKK-FENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            ++ AP F   L +   +NE D       + P GD +L V+W  +G P+    R S   E G   L I + I  D+G Y CVA +  G   S  +  ++  E+    +   +  Q V   EN                         + +G ++HLE  V P ND  L V+W +NG  +   SRF   F+ G+ +LDILY YPED+G Y  V +N+ GEA S  ++IT +P   +   S  + +    L+  F+ + Q ++     +  ++ D +AP F   LQ++ + EG   +F   +   + P   + W  + + V+     +   D     L+L             VATN  GQ      L     ++   D
Sbjct: 3716 DYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKG---SATSVGKIAVESSTQIDAPQVVQQLVDSVEN-------------------------ILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITD 4062

HSP 10 Score: 121.324 bits (303), Expect = 8.827e-28
Identity = 105/389 (26.99%), Postives = 170/389 (43.70%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE---SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTID-----EGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +E+E P+++   EN   V E   + L   V P+GD  L +EWL +G PL    RF   +E G  +L I++ +  DSGVY C A +  G+  ++        E  + ++ + +  + +Q+ E  P+  ++ E     + P F   LES   V +G  +HLE+   P  D  LKV W KNGQ +          + G+  LDI     + +GVYT    N+ GEA S+ S I       I+  +  +E S  +++  E              E + +  AP +D       ID     EG+   F  +I   + PR  + W  N   +   S      D  I  L++  T   + G + +R+   NP G+  S   L  +  +    D++H
Sbjct: 2782 REYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEKILYDSQHPVSWERIQELE-APKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVSTAS-IRVAGTGPILGNTR-HEESWKRIQILEA-----------PKEAEPEAPAPVYDHPAITTQIDDKECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRI--WNPEGEAVSSATLKCHGKDAILGDVQH 3154

HSP 11 Score: 99.7525 bits (247), Expect = 4.826e-21
Identity = 79/271 (29.15%), Postives = 118/271 (43.54%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-----------------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +GN  ++  +H     + K   +    Y   +  AP F + L+N I V E +  R  +QVAP  D  L VEW KD KP+L G RF +  + G+  LD+LY++ +D+G Y CVA ++ GQ                 +   P  +RV+   K + N+ + E     Q + +          Q+P F+  L +  +T NQ           PR  VT    W  NG     G RF   FD G   L +  +   D G    +A+N  GE  S+
Sbjct: 3927 HGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQN-IGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQK----------QAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVT----WFINGNQCLHGHRFKLNFD-GLHYLTVSKSRISDAGEVVAIARNTEGETIST 4181

HSP 12 Score: 94.3597 bits (233), Expect = 1.939e-19
Identity = 93/414 (22.46%), Postives = 177/414 (42.75%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS---QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKP 1606
            P+F++ L +   V E  P    +Q  P  D   SV+W  +G PL   +R     + G V LD+ Y++ ED+G Y  V K+  G+++++   +  T  A +  +    ++    Q+   P+ P         + P F + L+S   + +G    LE  + P ND  L+V W  N Q +   +  +   D G  +L I   Y    GVY+C A N+ G A +S + +     E ++    L+ S  + L+K +    E I+   R    + + + P + +G ++   + EG       ++     P   + W +N   +   + N+F     +G+  +  + +    + G +  R  A N  G+ ++   +T         D +H      ++++  L+   +  E+   + K KP
Sbjct: 2522 PKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAEPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLL----LDTSHPASLQKIQE--LEAIDKYARLDAPEREYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPL--RNANRFRQEYEFGNAILTIVHVL-PHDSGVYTCR--AWNTQGEASTSATVTTAGYEKILYDSQHPV---SWERIQELEAPKIVEEIEEIVQKEKP 2919

HSP 13 Score: 94.3597 bits (233), Expect = 2.223e-19
Identity = 79/271 (29.15%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-PEAKVDNNVNFIENQHVQQDENEPQY-----PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFEN-NIQEEIND 1168
            E   +E P F+  L++   + E     L +++ P  D +L V+W  + +PL+     ST  + G V L I       +GVY C A + SG N    A V V   E  + +  +    Q +Q+ E   +Y     P++    + P + +  E+   V +G  V L  +V+P  D  L+++W  NG  +   +RF   ++ G   L I++  P D GVYTC A N  GEA +S + +T    EKI+  S  +  S  ++++ E   I EEI +
Sbjct: 2647 EGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSG-NAVTSANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPERE--YEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTS-ATVTTAGYEKILYDSQ-HPVSWERIQELEAPKIVEEIEE 2912

HSP 14 Score: 92.0485 bits (227), Expect = 1.155e-18
Identity = 84/363 (23.14%), Postives = 145/363 (39.94%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            +AP F + L N++ + +  P   S Q+   G+   +++W KDG+PL    RF  V E G   L     I  D+G+Y  VAK+  G             EA+    +N    +  +  E  P+Y       ++P F+  ++  I V++G             D T++  W++N + +     +     +G   L I     ED   Y   A N  G+A SS + +   P    ++KS ++                    A   + D+    +P F   L D++  +G+  KF   +  +P  ++ W    + + + +  K   DG    LEL R T       ++V  N  G   S   LT++
Sbjct: 4528 KAPDFSARL-NDLRIQQNGPAEFSCQIG--GEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVG-------------EARCKARLNVNLQKTGKGAEEGPRY-------EAPRFTSQIQP-IVVDEGKGAQFSAQFSGFPDPTIR--WYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKA-SSVANLVLTPRSGRIAKSTISRG----------------GSASYQSSDKAAADSPHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4847

HSP 15 Score: 87.4261 bits (215), Expect = 2.687e-17
Identity = 102/385 (26.49%), Postives = 160/385 (41.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFI---INIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +P+F  +L+++  V E    RL  +  P  D +L + WL +GK +L  +R  T  + G   L+I      D G Y  VA +  G+        RV+    V  + +FI+ Q          Y  K   + +      P F  DL S   V +G  +HLET + P ND  L+V W  +G  +    ++      G+ +LDI  T   D G+Y+C A N  GE+E   +Q T I    IV KS+        L++FE + Q ++ D           D  P F   +Q    ++     F    I   + P   + W  + + +  ++ +  F   G ++  L  T   + G +    V  N  GQ  S   LT   I+    D KH
Sbjct:  936 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGE-------ARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESE---NQATII----IVPKSD--------LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYT--CVLFNSHGQAQSSAELTTVWIDTLQLDSKH 1294

HSP 16 Score: 72.0182 bits (175), Expect = 1.429e-12
Identity = 71/250 (28.40%), Postives = 106/250 (42.40%), Query Frame = 2 
Query:  356 GSVQSQY-----ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG-------------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            GS QS++     ++K  EAP F    ++  T+ + D ++L  + A  G   +S +W KD +P+ +G  ++   +     L I  +  ++ G Y C A +K G             Q  + PA   +    KVD     +E      +    Q  D   S  SP F   L+S   V +G +  LE    P  D  L++ W  NG+ +   SR     D G  AL+I      D G YT VA N  GEA  S
Sbjct:  787 GSFQSRFSLNVLQAKSPEAPEFTGKFQS-TTLYDGDSVKLYCKAAGEG---VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTTTLKGRVVVNSRQKFNGPAHREMITLRKVDK----VERSRTPVN----QLQDVSASKSSPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVS 1023
[Close]
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87])

HSP 1 Score: 173.711 bits (439), Expect = 1.739e-44
Identity = 131/440 (29.77%), Postives = 212/440 (48.18%), Query Frame = 2 
Query:  380 SKEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-------NNVNFIENQHVQQDENEPQYP--DKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES-SISQLKKFENNI-QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK------KVDVTPELTTALH-KPKPTAEKIVKIER 1636
            S + +AP+ +  L + +  + E D I L  +V P  D  L VEWL++G PL   +RF   FE G+V LDILY+  ED+G Y  V ++  G+ +S    + V P   +D       N  + +E+   Q  + + Q    D +  S  ++P F   L+ NI V +G     ET V P ND  LKV+W K+ + + LG RF +  D G+  LD+LY  P+D G Y CVA N  G+   S +++ C     +++ S + +   +S +KK   NI   E   A +  + Q    AP F   L+++ + E   A+F   +  +PR  + W IN  + +     K   DG ++YL +++        +  +A N  G+  S   L +   +++ Q    + P N +K    L+      + D    L  A    PKP A+K++ +ER
Sbjct: 3176 STQIDAPQVVQQLVDSVENILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQ-NIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMIS-AKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQKQ----APQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGEVVAIARNTEGETISTATLDIFQNDDFRQ--TKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVER 3604

HSP 2 Score: 170.244 bits (430), Expect = 2.783e-43
Identity = 112/400 (28.00%), Postives = 189/400 (47.25%), Query Frame = 2 
Query:  362 VQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-KVDNN-------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRAS--------IVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +Q  + S++   P+FLS ++      E       +++ P  D +L V WLKDG+PL    R       G V L +  +  ED+GVY CV  +  GQ QS+     V  +  ++D+        + ++++  +       + P++  S ++P F+++L   I V +   VH E  + P NDV + V+W+ NG  +    RF+ +FD GY ALD+LY YP+D G YT VA+N  GEA S+   +  +  EK++     +   + ++K+ E + ++ I +     ED+  D AP F   L D+ ++E        NIH   RA+        I W +N   +++ S  K   +     L++            V A+N LG+    C++TV        D +H
Sbjct:  540 IQFAHSSQDL-TPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV--VGTEKVLYLEPHHPEGLERIKELEQDRRQGIAE----VEDRTCDAAPKFLNDLPDIQLNEHE------NIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 926

HSP 3 Score: 151.754 bits (382), Expect = 2.359e-37
Identity = 119/404 (29.46%), Postives = 188/404 (46.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEIT-VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPE----AKVDNNVNFIENQHVQQDENEPQ-YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ--EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIV--------WSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK 1555
            E P F+  L+ ++  V E +PI L  QV P  DNSL + WL+DG+ L  G RF T ++ G+V LDIL    +D+G Y C A++  GQ ++  AT+R  P+      V +  ++   Q ++  +  PQ  PD     Q P F K L   I   +G  V+LE  V P +D +L  +W  NGQ +    RF    D GY AL+ILY YPED+G YT V +N  GEA+S+   I C       + S  + +S+ ++ + E  IQ  E + D        ++ + P+  K L   TID  + ++    +H   + + +        W  N   + + S  +   D     L++            +V  N  G+  + C   V+ + +   D  H       ++M  L+
Sbjct:  954 EPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQEVPDL--PHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST-VDINCGHTGGNFTDS-FHPNSLHRIAELETPIQRAEPLPD--------KEKEVPTIAKPL-PATIDSVHESQ---TLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRLKSILSDTAHPESLRRIREMEQLQ 1340

HSP 4 Score: 148.288 bits (373), Expect = 2.539e-36
Identity = 116/420 (27.62%), Postives = 202/420 (48.10%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-------QHVQQDEN-EPQYP-DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMAS---LKKVDVTPELT 1582
            KE E P     L   I +V+E   + L +QV P  DN+L  EWL +G PL   +R+  + + G+V LDI Y I EDSG Y  V  + +G+ ++       + E +VD   + + +       + +++ E  +P  P D   ++Q P F++ L      + +G +VH++ +V P ND +L+++W  +G+ +  GSR   + D GY  L+ L+ +PED G YTC A N  GEA ++   + C P   I   ++ +ESS  ++++ EN + E      R    +   Q P+F + L D     EG + +    +I ++  P   + W+ N + +   S  +F      +Y+ L      G+ +      A +  G+ A+ C +   +  +   D  H A     +++ +   L+ VD  PE T
Sbjct: 1216 KEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-------SCEFQVDRLKSILSDTAHPESLRRIREMEQLQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE------REPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVND-PTMRVTWTRNGQPLPEAS--RFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKT 1617

HSP 5 Score: 145.591 bits (366), Expect = 2.279e-35
Identity = 112/380 (29.47%), Postives = 172/380 (45.26%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLESNIT-VNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            F+ P F  NL ++    E   IRL  ++ P  D ++ V W ++G+PL   +RF       YV LDIL    EDSGVY C A S  G+  ++        ++ + + ++    + VQ+ EN  +    D      +P F   L S++  + +G  +HLE  V+P ND  L V W  NGQ ++ G RF    D GY ALDILY + +D G + CVA+N+ GEA++  +  T +P   I + S   ES     +K +  + E    A     D E D AP F + L  +   E  +A F   +   + P   I W  + + +++ +  K   D     L++            V A+N  G       LTV        D +H
Sbjct: 1484 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTI-ANFTVLPRGTIYTDSQHPES----WQKIQ--VLEAPRAAAPEKPDAEHD-APQFIEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTVTGNAVILGDTQH 1855

HSP 6 Score: 142.51 bits (358), Expect = 1.784e-34
Identity = 114/416 (27.40%), Postives = 185/416 (44.47%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV---DNNVNFIENQHVQQD--ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN-PYKKMASLKKVDVTPELTTALHKPK 1603
            ++FEAP  ++ L+ +  + E     L +Q  P  D S+ VEWLKDG+P+    R+  V + G+ VLDIL+ ++ D+G Y     + SG+  S   +  V+  + +     N    +   + +D     P+  ++     +P F + L + +   +GG  H     +P ND  L+V W+ +G+ +  GSR   +   GY  L+I  TYPED+G Y C A N  GEA +S +++TC P E I+S + L E   +  ++     + E     R      D   P F   L     + EG  A     +   + PR  I W  N + V   +  K   D     L+LT             ATN   Q  SD + T         +LK +      Y+  ++ ++ +   EL   +H+PK
Sbjct: 2670 QQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGE-ASTSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTS-TKLTCTPKEGIISATQLPERMANAGRRI---AEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATN---QHGSDEVST---------ELKVVGGGGVSYEWQSTAERKERITELEDWIHRPK 3068

HSP 7 Score: 133.265 bits (334), Expect = 1.431e-31
Identity = 98/306 (32.03%), Postives = 149/306 (48.69%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH---------VQQDENE--PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQ 1231
            G  SV+   E    EAP+F   L  +I V E + +   +++ PA D  ++VEW  +G PL    RF  +F+ GYV LD+LY+  +DSG Y  VA+++ G+ +SN   V  T        V ++E  H         ++QD  +   +  D+     +P F  DL  +I +N+   +H++    P ND T+ ++W  NG+ +  GSR   + + G+ ALDI     ED G Y+  A N  GEA      IT  P  +I+S +   ES    L K   N  E +N   R   ED+   + P+F   LQ
Sbjct:  673 GPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELVVGT------EKVLYLEPHHPEGLERIKELEQDRRQGIAEVEDR-TCDAAPKFLNDL-PDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEA-IRQCVITVTPAGQILSDTQHQES----LGKI--NYLENLNKYGRVEIEDKGPQEPPTFVVPLQ 963

HSP 8 Score: 128.257 bits (321), Expect = 5.744e-30
Identity = 109/381 (28.61%), Postives = 164/381 (43.04%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-----DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHS--HPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            E P FL+ LE+   V E  P+ L S   PA D  L V W K+G+PL       T  E G+  LDI  + E+ +GVY     +  G+  S  A++RV     +  N    E+    Q      E EP+ P        P  +  ++     N+G  VH E ++ P ND  L+V W +NG  ++ GS++    D G C LD+ YTYPED+GVY     N  GEA SS + + C   + I+      ES   I +++  +  ++E          D E    P F + L    ++ E   A F   +     P  +I W +N E + + S  K   D     +++ +         + VA N  G+  S   + V       QD
Sbjct: 2278 EKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVST-ASIRVAGTGPILGNTRHEESWKRIQILEAPKEAEPEAPAP--VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQKLEE---------ADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEAVSTATIEVQGKEVILQD 2644

HSP 9 Score: 128.257 bits (321), Expect = 6.105e-30
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKK-FENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            ++ AP F   L +   +NE D       + P GD +L V+W  +G P+    R S   E G   L I + I  D+G Y CVA +  G   S  +  ++  E+    +   +  Q V   EN                         + +G ++HLE  V P ND  L V+W +NG  +   SRF   F+ G+ +LDILY YPED+G Y  V +N+ GEA S  ++IT +P   +   S  + +    L+  F+ + Q ++     +  ++ D +AP F   LQ++ + EG   +F   +   + P   + W  + + V+     +   D     L+L             VATN  GQ      L     ++   D
Sbjct: 3077 DYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKG---SATSVGKIAVESSTQIDAPQVVQQLVDSVEN-------------------------ILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITD 3423

HSP 10 Score: 120.939 bits (302), Expect = 1.062e-27
Identity = 105/389 (26.99%), Postives = 170/389 (43.70%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE---SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTID-----EGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +E+E P+++   EN   V E   + L   V P+GD  L +EWL +G PL    RF   +E G  +L I++ +  DSGVY C A +  G+  ++        E  + ++ + +  + +Q+ E  P+  ++ E     + P F   LES   V +G  +HLE+   P  D  LKV W KNGQ +          + G+  LDI     + +GVYT    N+ GEA S+ S I       I+  +  +E S  +++  E              E + +  AP +D       ID     EG+   F  +I   + PR  + W  N   +   S      D  I  L++  T   + G + +R+   NP G+  S   L  +  +    D++H
Sbjct: 2143 REYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEKILYDSQHPVSWERIQELE-APKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVSTAS-IRVAGTGPILGNTR-HEESWKRIQILEA-----------PKEAEPEAPAPVYDHPAITTQIDDKECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRI--WNPEGEAVSSATLKCHGKDAILGDVQH 2515

HSP 11 Score: 99.7525 bits (247), Expect = 4.897e-21
Identity = 79/271 (29.15%), Postives = 118/271 (43.54%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-----------------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +GN  ++  +H     + K   +    Y   +  AP F + L+N I V E +  R  +QVAP  D  L VEW KD KP+L G RF +  + G+  LD+LY++ +D+G Y CVA ++ GQ                 +   P  +RV+   K + N+ + E     Q + +          Q+P F+  L +  +T NQ           PR  VT    W  NG     G RF   FD G   L +  +   D G    +A+N  GE  S+
Sbjct: 3288 HGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQN-IGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQK----------QAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVT----WFINGNQCLHGHRFKLNFD-GLHYLTVSKSRISDAGEVVAIARNTEGETIST 3542

HSP 12 Score: 94.3597 bits (233), Expect = 2.002e-19
Identity = 93/414 (22.46%), Postives = 177/414 (42.75%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS---QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKP 1606
            P+F++ L +   V E  P    +Q  P  D   SV+W  +G PL   +R     + G V LD+ Y++ ED+G Y  V K+  G+++++   +  T  A +  +    ++    Q+   P+ P         + P F + L+S   + +G    LE  + P ND  L+V W  N Q +   +  +   D G  +L I   Y    GVY+C A N+ G A +S + +     E ++    L+ S  + L+K +    E I+   R    + + + P + +G ++   + EG       ++     P   + W +N   +   + N+F     +G+  +  + +    + G +  R  A N  G+ ++   +T         D +H      ++++  L+   +  E+   + K KP
Sbjct: 1883 PKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAEPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLL----LDTSHPASLQKIQE--LEAIDKYARLDAPEREYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPL--RNANRFRQEYEFGNAILTIVHVL-PHDSGVYTCR--AWNTQGEASTSATVTTAGYEKILYDSQHPV---SWERIQELEAPKIVEEIEEIVQKEKP 2280

HSP 13 Score: 94.3597 bits (233), Expect = 2.237e-19
Identity = 79/271 (29.15%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-PEAKVDNNVNFIENQHVQQDENEPQY-----PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFEN-NIQEEIND 1168
            E   +E P F+  L++   + E     L +++ P  D +L V+W  + +PL+     ST  + G V L I       +GVY C A + SG N    A V V   E  + +  +    Q +Q+ E   +Y     P++    + P + +  E+   V +G  V L  +V+P  D  L+++W  NG  +   +RF   ++ G   L I++  P D GVYTC A N  GEA +S + +T    EKI+  S  +  S  ++++ E   I EEI +
Sbjct: 2008 EGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSG-NAVTSANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPERE--YEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTS-ATVTTAGYEKILYDSQ-HPVSWERIQELEAPKIVEEIEE 2273

HSP 14 Score: 91.6633 bits (226), Expect = 1.288e-18
Identity = 84/363 (23.14%), Postives = 145/363 (39.94%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            +AP F + L N++ + +  P   S Q+   G+   +++W KDG+PL    RF  V E G   L     I  D+G+Y  VAK+  G             EA+    +N    +  +  E  P+Y       ++P F+  ++  I V++G             D T++  W++N + +     +     +G   L I     ED   Y   A N  G+A SS + +   P    ++KS ++                    A   + D+    +P F   L D++  +G+  KF   +  +P  ++ W    + + + +  K   DG    LEL R T       ++V  N  G   S   LT++
Sbjct: 4004 KAPDFSARL-NDLRIQQNGPAEFSCQIG--GEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVG-------------EARCKARLNVNLQKTGKGAEEGPRY-------EAPRFTSQIQP-IVVDEGKGAQFSAQFSGFPDPTIR--WYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKA-SSVANLVLTPRSGRIAKSTISRG----------------GSASYQSSDKAAADSPHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4323

HSP 15 Score: 87.4261 bits (215), Expect = 2.898e-17
Identity = 102/385 (26.49%), Postives = 160/385 (41.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFI---INIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +P+F  +L+++  V E    RL  +  P  D +L + WL +GK +L  +R  T  + G   L+I      D G Y  VA +  G+        RV+    V  + +FI+ Q          Y  K   + +      P F  DL S   V +G  +HLET + P ND  L+V W  +G  +    ++      G+ +LDI  T   D G+Y+C A N  GE+E   +Q T I    IV KS+        L++FE + Q ++ D           D  P F   +Q    ++     F    I   + P   + W  + + +  ++ +  F   G ++  L  T   + G +    V  N  GQ  S   LT   I+    D KH
Sbjct:  297 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGE-------ARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESE---NQATII----IVPKSD--------LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYT--CVLFNSHGQAQSSAELTTVWIDTLQLDSKH 655

HSP 16 Score: 72.4034 bits (176), Expect = 1.259e-12
Identity = 71/250 (28.40%), Postives = 106/250 (42.40%), Query Frame = 2 
Query:  356 GSVQSQY-----ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG-------------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            GS QS++     ++K  EAP F    ++  T+ + D ++L  + A  G   +S +W KD +P+ +G  ++   +     L I  +  ++ G Y C A +K G             Q  + PA   +    KVD     +E      +    Q  D   S  SP F   L+S   V +G +  LE    P  D  L++ W  NG+ +   SR     D G  AL+I      D G YT VA N  GEA  S
Sbjct:  148 GSFQSRFSLNVLQAKSPEAPEFTGKFQS-TTLYDGDSVKLYCKAAGEG---VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTTTLKGRVVVNSRQKFNGPAHREMITLRKVDK----VERSRTPVN----QLQDVSASKSSPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVS 384
[Close]
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87])

HSP 1 Score: 173.711 bits (439), Expect = 1.747e-44
Identity = 131/440 (29.77%), Postives = 212/440 (48.18%), Query Frame = 2 
Query:  380 SKEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-------NNVNFIENQHVQQDENEPQYP--DKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES-SISQLKKFENNI-QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK------KVDVTPELTTALH-KPKPTAEKIVKIER 1636
            S + +AP+ +  L + +  + E D I L  +V P  D  L VEWL++G PL   +RF   FE G+V LDILY+  ED+G Y  V ++  G+ +S    + V P   +D       N  + +E+   Q  + + Q    D +  S  ++P F   L+ NI V +G     ET V P ND  LKV+W K+ + + LG RF +  D G+  LD+LY  P+D G Y CVA N  G+   S +++ C     +++ S + +   +S +KK   NI   E   A +  + Q    AP F   L+++ + E   A+F   +  +PR  + W IN  + +     K   DG ++YL +++        +  +A N  G+  S   L +   +++ Q    + P N +K    L+      + D    L  A    PKP A+K++ +ER
Sbjct: 3176 STQIDAPQVVQQLVDSVENILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQ-NIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMIS-AKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQKQ----APQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGEVVAIARNTEGETISTATLDIFQNDDFRQ--TKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVER 3604

HSP 2 Score: 169.859 bits (429), Expect = 3.227e-43
Identity = 112/400 (28.00%), Postives = 189/400 (47.25%), Query Frame = 2 
Query:  362 VQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-KVDNN-------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRAS--------IVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +Q  + S++   P+FLS ++      E       +++ P  D +L V WLKDG+PL    R       G V L +  +  ED+GVY CV  +  GQ QS+     V  +  ++D+        + ++++  +       + P++  S ++P F+++L   I V +   VH E  + P NDV + V+W+ NG  +    RF+ +FD GY ALD+LY YP+D G YT VA+N  GEA S+   +  +  EK++     +   + ++K+ E + ++ I +     ED+  D AP F   L D+ ++E        NIH   RA+        I W +N   +++ S  K   +     L++            V A+N LG+    C++TV        D +H
Sbjct:  540 IQFAHSSQDL-TPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV--VGTEKVLYLEPHHPEGLERIKELEQDRRQGIAE----VEDRTCDAAPKFLNDLPDIQLNEHE------NIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 926

HSP 3 Score: 151.369 bits (381), Expect = 2.880e-37
Identity = 119/404 (29.46%), Postives = 188/404 (46.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEIT-VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPE----AKVDNNVNFIENQHVQQDENEPQ-YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ--EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIV--------WSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLK 1555
            E P F+  L+ ++  V E +PI L  QV P  DNSL + WL+DG+ L  G RF T ++ G+V LDIL    +D+G Y C A++  GQ ++  AT+R  P+      V +  ++   Q ++  +  PQ  PD     Q P F K L   I   +G  V+LE  V P +D +L  +W  NGQ +    RF    D GY AL+ILY YPED+G YT V +N  GEA+S+   I C       + S  + +S+ ++ + E  IQ  E + D        ++ + P+  K L   TID  + ++    +H   + + +        W  N   + + S  +   D     L++            +V  N  G+  + C   V+ + +   D  H       ++M  L+
Sbjct:  954 EPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETV-ATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQEVPDL--PHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST-VDINCGHTGGNFTDS-FHPNSLHRIAELETPIQRAEPLPD--------KEKEVPTIAKPL-PATIDSVHESQ---TLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSCEFQVDRLKSILSDTAHPESLRRIREMEQLQ 1340

HSP 4 Score: 148.288 bits (373), Expect = 2.714e-36
Identity = 116/420 (27.62%), Postives = 202/420 (48.10%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEI-TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-------QHVQQDEN-EPQYP-DKHESSQSPYFSKDLESNI-TVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHN--IRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMAS---LKKVDVTPELT 1582
            KE E P     L   I +V+E   + L +QV P  DN+L  EWL +G PL   +R+  + + G+V LDI Y I EDSG Y  V  + +G+ ++       + E +VD   + + +       + +++ E  +P  P D   ++Q P F++ L      + +G +VH++ +V P ND +L+++W  +G+ +  GSR   + D GY  L+ L+ +PED G YTC A N  GEA ++   + C P   I   ++ +ESS  ++++ EN + E      R    +   Q P+F + L D     EG + +    +I ++  P   + W+ N + +   S  +F      +Y+ L      G+ +      A +  G+ A+ C +   +  +   D  H A     +++ +   L+ VD  PE T
Sbjct: 1216 KEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-------SCEFQVDRLKSILSDTAHPESLRRIREMEQLQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE------REPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVND-PTMRVTWTRNGQPLPEAS--RFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKT 1617

HSP 5 Score: 145.206 bits (365), Expect = 2.636e-35
Identity = 112/380 (29.47%), Postives = 172/380 (45.26%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLESNIT-VNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            F+ P F  NL ++    E   IRL  ++ P  D ++ V W ++G+PL   +RF       YV LDIL    EDSGVY C A S  G+  ++        ++ + + ++    + VQ+ EN  +    D      +P F   L S++  + +G  +HLE  V+P ND  L V W  NGQ ++ G RF    D GY ALDILY + +D G + CVA+N+ GEA++  +  T +P   I + S   ES     +K +  + E    A     D E D AP F + L  +   E  +A F   +   + P   I W  + + +++ +  K   D     L++            V A+N  G       LTV        D +H
Sbjct: 1484 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCTVKCAAGKSLLLDTMHDASWKRVQEIENREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTI-ANFTVLPRGTIYTDSQHPES----WQKIQ--VLEAPRAAAPEKPDAEHD-APQFIEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTVTGNAVILGDTQH 1855

HSP 6 Score: 142.51 bits (358), Expect = 2.009e-34
Identity = 114/416 (27.40%), Postives = 185/416 (44.47%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV---DNNVNFIENQHVQQD--ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN-PYKKMASLKKVDVTPELTTALHKPK 1603
            ++FEAP  ++ L+ +  + E     L +Q  P  D S+ VEWLKDG+P+    R+  V + G+ VLDIL+ ++ D+G Y     + SG+  S   +  V+  + +     N    +   + +D     P+  ++     +P F + L + +   +GG  H     +P ND  L+V W+ +G+ +  GSR   +   GY  L+I  TYPED+G Y C A N  GEA +S +++TC P E I+S + L E   +  ++     + E     R      D   P F   L     + EG  A     +   + PR  I W  N + V   +  K   D     L+LT             ATN   Q  SD + T         +LK +      Y+  ++ ++ +   EL   +H+PK
Sbjct: 2670 QQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGE-ASTSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTS-TKLTCTPKEGIISATQLPERMANAGRRI---AEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATN---QHGSDEVST---------ELKVVGGGGVSYEWQSTAERKERITELEDWIHRPK 3068

HSP 7 Score: 133.265 bits (334), Expect = 1.698e-31
Identity = 98/306 (32.03%), Postives = 149/306 (48.69%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH---------VQQDENE--PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQ 1231
            G  SV+   E    EAP+F   L  +I V E + +   +++ PA D  ++VEW  +G PL    RF  +F+ GYV LD+LY+  +DSG Y  VA+++ G+ +SN   V  T        V ++E  H         ++QD  +   +  D+     +P F  DL  +I +N+   +H++    P ND T+ ++W  NG+ +  GSR   + + G+ ALDI     ED G Y+  A N  GEA      IT  P  +I+S +   ES    L K   N  E +N   R   ED+   + P+F   LQ
Sbjct:  673 GPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELVVGT------EKVLYLEPHHPEGLERIKELEQDRRQGIAEVEDR-TCDAAPKFLNDL-PDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEA-IRQCVITVTPAGQILSDTQHQES----LGKI--NYLENLNKYGRVEIEDKGPQEPPTFVVPLQ 963

HSP 8 Score: 128.257 bits (321), Expect = 6.030e-30
Identity = 109/381 (28.61%), Postives = 164/381 (43.04%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-----DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHS--HPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            E P FL+ LE+   V E  P+ L S   PA D  L V W K+G+PL       T  E G+  LDI  + E+ +GVY     +  G+  S  A++RV     +  N    E+    Q      E EP+ P        P  +  ++     N+G  VH E ++ P ND  L+V W +NG  ++ GS++    D G C LD+ YTYPED+GVY     N  GEA SS + + C   + I+      ES   I +++  +  ++E          D E    P F + L    ++ E   A F   +     P  +I W +N E + + S  K   D     +++ +         + VA N  G+  S   + V       QD
Sbjct: 2278 EKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVST-ASIRVAGTGPILGNTRHEESWKRIQILEAPKEAEPEAPAP--VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQKLEE---------ADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEAVSTATIEVQGKEVILQD 2644

HSP 9 Score: 127.872 bits (320), Expect = 7.301e-30
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKK-FENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            ++ AP F   L +   +NE D       + P GD +L V+W  +G P+    R S   E G   L I + I  D+G Y CVA +  G   S  +  ++  E+    +   +  Q V   EN                         + +G ++HLE  V P ND  L V+W +NG  +   SRF   F+ G+ +LDILY YPED+G Y  V +N+ GEA S  ++IT +P   +   S  + +    L+  F+ + Q ++     +  ++ D +AP F   LQ++ + EG   +F   +   + P   + W  + + V+     +   D     L+L             VATN  GQ      L     ++   D
Sbjct: 3077 DYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKG---SATSVGKIAVESSTQIDAPQVVQQLVDSVEN-------------------------ILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITD 3423

HSP 10 Score: 120.939 bits (302), Expect = 1.106e-27
Identity = 105/389 (26.99%), Postives = 170/389 (43.70%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE---SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTID-----EGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +E+E P+++   EN   V E   + L   V P+GD  L +EWL +G PL    RF   +E G  +L I++ +  DSGVY C A +  G+  ++        E  + ++ + +  + +Q+ E  P+  ++ E     + P F   LES   V +G  +HLE+   P  D  LKV W KNGQ +          + G+  LDI     + +GVYT    N+ GEA S+ S I       I+  +  +E S  +++  E              E + +  AP +D       ID     EG+   F  +I   + PR  + W  N   +   S      D  I  L++  T   + G + +R+   NP G+  S   L  +  +    D++H
Sbjct: 2143 REYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEKILYDSQHPVSWERIQELE-APKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVSTAS-IRVAGTGPILGNTR-HEESWKRIQILEA-----------PKEAEPEAPAPVYDHPAITTQIDDKECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRI--WNPEGEAVSSATLKCHGKDAILGDVQH 2515

HSP 11 Score: 99.3673 bits (246), Expect = 5.552e-21
Identity = 79/271 (29.15%), Postives = 118/271 (43.54%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-----------------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +GN  ++  +H     + K   +    Y   +  AP F + L+N I V E +  R  +QVAP  D  L VEW KD KP+L G RF +  + G+  LD+LY++ +D+G Y CVA ++ GQ                 +   P  +RV+   K + N+ + E     Q + +          Q+P F+  L +  +T NQ           PR  VT    W  NG     G RF   FD G   L +  +   D G    +A+N  GE  S+
Sbjct: 3288 HGNQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQN-IGVLEGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMISAKLACQGASHVITDSQMPQGLRVSNVKKDNKNIYWSEQGGAVQPKQK----------QAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVT----WFINGNQCLHGHRFKLNFD-GLHYLTVSKSRISDAGEVVAIARNTEGETIST 3542

HSP 12 Score: 94.3597 bits (233), Expect = 2.175e-19
Identity = 93/414 (22.46%), Postives = 177/414 (42.75%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS---QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKP 1606
            P+F++ L +   V E  P    +Q  P  D   SV+W  +G PL   +R     + G V LD+ Y++ ED+G Y  V K+  G+++++   +  T  A +  +    ++    Q+   P+ P         + P F + L+S   + +G    LE  + P ND  L+V W  N Q +   +  +   D G  +L I   Y    GVY+C A N+ G A +S + +     E ++    L+ S  + L+K +    E I+   R    + + + P + +G ++   + EG       ++     P   + W +N   +   + N+F     +G+  +  + +    + G +  R  A N  G+ ++   +T         D +H      ++++  L+   +  E+   + K KP
Sbjct: 1883 PKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAEPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLL----LDTSHPASLQKIQE--LEAIDKYARLDAPEREYEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPL--RNANRFRQEYEFGNAILTIVHVL-PHDSGVYTCR--AWNTQGEASTSATVTTAGYEKILYDSQHPV---SWERIQELEAPKIVEEIEEIVQKEKP 2280

HSP 13 Score: 93.9745 bits (232), Expect = 2.369e-19
Identity = 79/271 (29.15%), Postives = 129/271 (47.60%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-PEAKVDNNVNFIENQHVQQDENEPQY-----PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFEN-NIQEEIND 1168
            E   +E P F+  L++   + E     L +++ P  D +L V+W  + +PL+     ST  + G V L I       +GVY C A + SG N    A V V   E  + +  +    Q +Q+ E   +Y     P++    + P + +  E+   V +G  V L  +V+P  D  L+++W  NG  +   +RF   ++ G   L I++  P D GVYTC A N  GEA +S + +T    EKI+  S  +  S  ++++ E   I EEI +
Sbjct: 2008 EGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSG-NAVTSANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPERE--YEKPQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTS-ATVTTAGYEKILYDSQ-HPVSWERIQELEAPKIVEEIEE 2273

HSP 14 Score: 91.6633 bits (226), Expect = 1.485e-18
Identity = 84/363 (23.14%), Postives = 145/363 (39.94%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            +AP F + L N++ + +  P   S Q+   G+   +++W KDG+PL    RF  V E G   L     I  D+G+Y  VAK+  G             EA+    +N    +  +  E  P+Y       ++P F+  ++  I V++G             D T++  W++N + +     +     +G   L I     ED   Y   A N  G+A SS + +   P    ++KS ++                    A   + D+    +P F   L D++  +G+  KF   +  +P  ++ W    + + + +  K   DG    LEL R T       ++V  N  G   S   LT++
Sbjct: 3889 KAPDFSARL-NDLRIQQNGPAEFSCQIG--GEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVG-------------EARCKARLNVNLQKTGKGAEEGPRY-------EAPRFTSQIQP-IVVDEGKGAQFSAQFSGFPDPTIR--WYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKA-SSVANLVLTPRSGRIAKSTISRG----------------GSASYQSSDKAAADSPHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4208

HSP 15 Score: 87.0409 bits (214), Expect = 3.370e-17
Identity = 102/385 (26.49%), Postives = 160/385 (41.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFI---INIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +P+F  +L+++  V E    RL  +  P  D +L + WL +GK +L  +R  T  + G   L+I      D G Y  VA +  G+        RV+    V  + +FI+ Q          Y  K   + +      P F  DL S   V +G  +HLET + P ND  L+V W  +G  +    ++      G+ +LDI  T   D G+Y+C A N  GE+E   +Q T I    IV KS+        L++FE + Q ++ D           D  P F   +Q    ++     F    I   + P   + W  + + +  ++ +  F   G ++  L  T   + G +    V  N  GQ  S   LT   I+    D KH
Sbjct:  297 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGE-------ARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDLPNFHSDLRSQ-EVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESE---NQATII----IVPKSD--------LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYT--CVLFNSHGQAQSSAELTTVWIDTLQLDSKH 655

HSP 16 Score: 72.0182 bits (175), Expect = 1.391e-12
Identity = 71/250 (28.40%), Postives = 106/250 (42.40%), Query Frame = 2 
Query:  356 GSVQSQY-----ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG-------------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            GS QS++     ++K  EAP F    ++  T+ + D ++L  + A  G   +S +W KD +P+ +G  ++   +     L I  +  ++ G Y C A +K G             Q  + PA   +    KVD     +E      +    Q  D   S  SP F   L+S   V +G +  LE    P  D  L++ W  NG+ +   SR     D G  AL+I      D G YT VA N  GEA  S
Sbjct:  148 GSFQSRFSLNVLQAKSPEAPEFTGKFQS-TTLYDGDSVKLYCKAAGEG---VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTTTLKGRVVVNSRQKFNGPAHREMITLRKVDK----VERSRTPVN----QLQDVSASKSSPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVS 384
[Close]
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332683)

HSP 1 Score: 176.407 bits (446), Expect = 3.621e-45
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3808 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDL--FDPDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4192

HSP 2 Score: 160.229 bits (404), Expect = 5.489e-40
Identity = 112/380 (29.47%), Postives = 183/380 (48.16%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S   +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V   + ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1022 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSAC-VRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPV--KTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1387

HSP 3 Score: 158.688 bits (400), Expect = 1.713e-39
Identity = 121/397 (30.48%), Postives = 192/397 (48.36%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N I   E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G ++ +  ++ C   E+IV+ +  NE+ + Q+   E        D  R T  +E D    QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1288 MQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIG-SDETQVELQCRSGEQIVTVTQ-NEAGLEQIHYLE--------DRSRYTRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1661

HSP 4 Score: 145.591 bits (366), Expect = 2.543e-35
Identity = 117/406 (28.82%), Postives = 183/406 (45.07%), Query Frame = 2 
Query:  368 SQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
            S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1808 SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEA-INSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL-RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVV------EEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2193

HSP 5 Score: 145.206 bits (365), Expect = 3.712e-35
Identity = 112/389 (28.79%), Postives = 189/389 (48.59%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++ L+++   +  +      A NPLG+  +   + V S  +   D +H
Sbjct: 2087 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEA-VNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVS-LDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVSEASIQMDTQH 2460

HSP 6 Score: 143.28 bits (360), Expect = 1.713e-34
Identity = 105/371 (28.30%), Postives = 176/371 (47.44%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVIS 1336
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N + + S
Sbjct: 3502 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPS 3855

HSP 7 Score: 140.198 bits (352), Expect = 1.549e-33
Identity = 107/381 (28.08%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      ++    V  + +    Q +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1252

HSP 8 Score: 140.198 bits (352), Expect = 1.577e-33
Identity = 103/390 (26.41%), Postives = 181/390 (46.41%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-------------PEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G   +   + +VT             PE      +  +E   +      P+ P    E    P F+  + +++TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F       Y+ +        + G++  R  ATN  G+  +  I+ V+
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG---TATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRFQPIYDFGYVAMNFGWVYPEDSGEYVCR--ATNLYGKDETRAIIKVS 4047

HSP 9 Score: 132.109 bits (331), Expect = 4.495e-31
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3261 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTAT-MKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3675

HSP 10 Score: 131.724 bits (330), Expect = 5.888e-31
Identity = 106/382 (27.75%), Postives = 168/382 (43.98%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT             ATN  G   +   L          D +H
Sbjct: 2485 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKE------GRPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQH 2857

HSP 11 Score: 129.798 bits (325), Expect = 2.997e-30
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  611 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 859

HSP 12 Score: 127.872 bits (320), Expect = 9.857e-30
Identity = 101/386 (26.17%), Postives = 187/386 (48.45%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I  +  +  +     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1556 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPV-RDVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI--VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTVY-AEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1928

HSP 13 Score: 122.865 bits (307), Expect = 4.588e-28
Identity = 89/308 (28.90%), Postives = 141/308 (45.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSI----SQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2356 PVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQ--HVLLEAQVEPRA 2650

HSP 14 Score: 115.931 bits (289), Expect = 6.033e-26
Identity = 100/386 (25.91%), Postives = 172/386 (44.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3773

HSP 15 Score: 113.235 bits (282), Expect = 4.524e-25
Identity = 98/376 (26.06%), Postives = 163/376 (43.35%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQ-HVQQDENEPQYPDKHES-------SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVN 1480
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +   Q    Q   E + P   ++       ++ P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + + 
Sbjct: 3128 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKI-ANLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKIQ 3494

HSP 16 Score: 110.538 bits (275), Expect = 2.526e-24
Identity = 92/372 (24.73%), Postives = 153/372 (41.13%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++      +T      + P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E         A N  G+  +   L   S ++   D
Sbjct: 3029 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQA-----DTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRASLKCASKSSLILD 3364

HSP 17 Score: 107.842 bits (268), Expect = 1.983e-23
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2888 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3122

HSP 18 Score: 88.1965 bits (217), Expect = 2.227e-17
Identity = 101/412 (24.51%), Postives = 165/412 (40.05%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI 1516
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV ++   GQ+ S   LTV      A  L+ I
Sbjct: 4471 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSST--GQVESSAKLTVKKRRISAFQLRTI 4805

HSP 19 Score: 69.707 bits (169), Expect = 1.078e-11
Identity = 93/395 (23.54%), Postives = 154/395 (38.99%), Query Frame = 2 
Query:  338 GRPKGHGSV--QSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTED------QEDDQAPSFDKGL-QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF--YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            G  K  GSV    Q   ++ + PRF+  LE +     E  + +   +  +   S S +W    +P+ +      V +     L I     +  G + C A++  G   S  ATV + P+                         ++ E  +SP F ++L   + V  G A+ L   V  +   T KV+W+ N + ++         D +G C L I   +PE++G Y CVA N  G               K VSK+N+      +++ FE     EI     + ED        D+QAP   K L + +   EG  AK  + +   P+  + W  ++E++ +    +   + DGT + L +          I   A NPLG   +  +  V  I
Sbjct: 6819 GEAKSSGSVVVSDQKPDEQIKPPRFIQPLEPKYFGEHE--VAIIEAIVESEPLS-SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAENVGGSVTST-ATVNLIPQ-------------------------EEAEEFESPRFVEELVQPVEVMDGEALLLTCQVTGKP--TPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIG---------------KSVSKTNV------KIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAVEGI 7160

HSP 20 Score: 57.3806 bits (137), Expect = 5.917e-8
Identity = 73/337 (21.66%), Postives = 116/337 (34.42%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +   +P           P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4419 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKGGIQP-----------PSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4698
[Close]
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0072848)

HSP 1 Score: 176.022 bits (445), Expect = 4.170e-45
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3808 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFD--PDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4192

HSP 2 Score: 161.77 bits (408), Expect = 2.006e-40
Identity = 112/380 (29.47%), Postives = 184/380 (48.42%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S + +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V   + ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1022 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTS-ACVRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPV--KTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1387

HSP 3 Score: 159.073 bits (401), Expect = 1.148e-39
Identity = 119/393 (30.28%), Postives = 191/393 (48.60%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N  T+ E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G  E +  ++ C   E+IV+ +  NE+ + Q+   E+  +       R   D+   QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1288 MQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDE-TQVELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRY----TRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1661

HSP 4 Score: 147.902 bits (372), Expect = 4.531e-36
Identity = 117/407 (28.75%), Postives = 183/407 (44.96%), Query Frame = 2 
Query:  365 QSQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
             S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1807 HSRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGE-AINSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPLR-NAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPP------KPVVEEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2193

HSP 5 Score: 146.362 bits (368), Expect = 1.515e-35
Identity = 112/389 (28.79%), Postives = 189/389 (48.59%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++ L+++   +  +      A NPLG+  +   + V S  +   D +H
Sbjct: 2087 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGE-AVNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVS-LDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVSEASIQMDTQH 2460

HSP 6 Score: 144.05 bits (362), Expect = 7.996e-35
Identity = 105/371 (28.30%), Postives = 176/371 (47.44%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVIS 1336
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N + + S
Sbjct: 3502 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPS 3855

HSP 7 Score: 140.969 bits (354), Expect = 7.060e-34
Identity = 107/381 (28.08%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      ++    V  + +    Q +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1252

HSP 8 Score: 140.198 bits (352), Expect = 1.293e-33
Identity = 94/341 (27.57%), Postives = 160/341 (46.92%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKF 1354
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G          + + N       PE      +  +E   +      P+ P    E    P F+  + S +TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRF 4003

HSP 9 Score: 134.42 bits (337), Expect = 8.541e-32
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3261 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3675

HSP 10 Score: 133.65 bits (335), Expect = 1.643e-31
Identity = 106/382 (27.75%), Postives = 168/382 (43.98%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT             ATN  G   +   L          D +H
Sbjct: 2485 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKEG------RPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQH 2857

HSP 11 Score: 129.413 bits (324), Expect = 3.348e-30
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  611 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 859

HSP 12 Score: 129.413 bits (324), Expect = 3.558e-30
Identity = 101/386 (26.17%), Postives = 187/386 (48.45%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I  +  +  +     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1556 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPVR-DVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI--VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTVY-AEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1928

HSP 13 Score: 122.865 bits (307), Expect = 3.759e-28
Identity = 89/310 (28.71%), Postives = 142/310 (45.81%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSI----SQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            + P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2354 QRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQ--HVLLEAQVEPRA 2650

HSP 14 Score: 117.472 bits (293), Expect = 1.602e-26
Identity = 100/387 (25.84%), Postives = 173/387 (44.70%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3399 KAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3773

HSP 15 Score: 115.546 bits (288), Expect = 6.708e-26
Identity = 99/375 (26.40%), Postives = 162/375 (43.20%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQ-HVQQDENEPQYPDKHESSQS-------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTV 1477
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +   Q    Q   E + P   ++  S       P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + +
Sbjct: 3128 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKIA-NLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKI 3493

HSP 16 Score: 112.849 bits (281), Expect = 5.212e-25
Identity = 86/338 (25.44%), Postives = 143/338 (42.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTE 1402
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++      +T      + P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E
Sbjct: 3029 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQA-----DTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYE 3330

HSP 17 Score: 108.227 bits (269), Expect = 1.223e-23
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2888 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3122

HSP 18 Score: 85.1149 bits (209), Expect = 1.764e-16
Identity = 100/401 (24.94%), Postives = 162/401 (40.40%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNK---FYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV  T+  GQ+ S   LTV S
Sbjct: 4471 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRV--TSSTGQVESSAKLTVKS 4794

HSP 19 Score: 55.4546 bits (132), Expect = 2.179e-7
Identity = 73/337 (21.66%), Postives = 116/337 (34.42%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +   +P           P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4419 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKGGIQP-----------PSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4698
[Close]
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0309042)

HSP 1 Score: 176.022 bits (445), Expect = 4.648e-45
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3823 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFD--PDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4207

HSP 2 Score: 161.384 bits (407), Expect = 2.135e-40
Identity = 112/380 (29.47%), Postives = 184/380 (48.42%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S + +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V   + ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1037 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTS-ACVRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPV--KTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1402

HSP 3 Score: 159.073 bits (401), Expect = 1.301e-39
Identity = 119/393 (30.28%), Postives = 191/393 (48.60%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N  T+ E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G  E +  ++ C   E+IV+ +  NE+ + Q+   E+  +       R   D+   QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1303 MQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDE-TQVELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRY----TRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1676

HSP 4 Score: 147.902 bits (372), Expect = 4.994e-36
Identity = 117/407 (28.75%), Postives = 183/407 (44.96%), Query Frame = 2 
Query:  365 QSQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
             S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1822 HSRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGE-AINSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPLR-NAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPP------KPVVEEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2208

HSP 5 Score: 146.362 bits (368), Expect = 1.700e-35
Identity = 112/389 (28.79%), Postives = 189/389 (48.59%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++ L+++   +  +      A NPLG+  +   + V S  +   D +H
Sbjct: 2102 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGE-AVNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVS-LDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVSEASIQMDTQH 2475

HSP 6 Score: 144.05 bits (362), Expect = 8.811e-35
Identity = 105/371 (28.30%), Postives = 176/371 (47.44%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVIS 1336
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N + + S
Sbjct: 3517 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPS 3870

HSP 7 Score: 140.969 bits (354), Expect = 7.188e-34
Identity = 107/381 (28.08%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      ++    V  + +    Q +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  905 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1267

HSP 8 Score: 140.198 bits (352), Expect = 1.375e-33
Identity = 94/341 (27.57%), Postives = 160/341 (46.92%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------QNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKF 1354
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G          + + N       PE      +  +E   +      P+ P    E    P F+  + S +TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F
Sbjct: 3691 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRF 4018

HSP 9 Score: 134.42 bits (337), Expect = 9.243e-32
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3276 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3690

HSP 10 Score: 133.65 bits (335), Expect = 1.673e-31
Identity = 106/382 (27.75%), Postives = 168/382 (43.98%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT             ATN  G   +   L          D +H
Sbjct: 2500 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKEG------RPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQH 2872

HSP 11 Score: 129.413 bits (324), Expect = 3.408e-30
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  626 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 874

HSP 12 Score: 129.028 bits (323), Expect = 3.882e-30
Identity = 101/386 (26.17%), Postives = 187/386 (48.45%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I  +  +  +     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1571 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPVR-DVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI--VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTVY-AEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1943

HSP 13 Score: 122.865 bits (307), Expect = 3.961e-28
Identity = 89/310 (28.71%), Postives = 142/310 (45.81%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSI----SQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            + P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2369 QRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQ--HVLLEAQVEPRA 2665

HSP 14 Score: 117.472 bits (293), Expect = 1.747e-26
Identity = 100/387 (25.84%), Postives = 173/387 (44.70%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3414 KAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3788

HSP 15 Score: 115.546 bits (288), Expect = 6.710e-26
Identity = 99/375 (26.40%), Postives = 162/375 (43.20%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQ-HVQQDENEPQYPDKHESSQS-------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTV 1477
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +   Q    Q   E + P   ++  S       P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + +
Sbjct: 3143 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKIA-NLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKI 3508

HSP 16 Score: 112.849 bits (281), Expect = 5.443e-25
Identity = 86/338 (25.44%), Postives = 143/338 (42.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTE 1402
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++      +T      + P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E
Sbjct: 3044 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQA-----DTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYE 3345

HSP 17 Score: 108.227 bits (269), Expect = 1.299e-23
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2903 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3137

HSP 18 Score: 85.1149 bits (209), Expect = 1.795e-16
Identity = 100/401 (24.94%), Postives = 162/401 (40.40%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNK---FYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV  T+  GQ+ S   LTV S
Sbjct: 4486 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRV--TSSTGQVESSAKLTVKS 4809

HSP 19 Score: 55.4546 bits (132), Expect = 2.143e-7
Identity = 73/337 (21.66%), Postives = 116/337 (34.42%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +   +P           P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4434 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKGGIQP-----------PSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4713
[Close]
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332677)

HSP 1 Score: 176.022 bits (445), Expect = 4.830e-45
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3808 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDL--FDPDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4192

HSP 2 Score: 159.844 bits (403), Expect = 9.277e-40
Identity = 112/380 (29.47%), Postives = 183/380 (48.16%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S   +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V   + ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1022 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSAC-VRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPV--KTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1387

HSP 3 Score: 158.688 bits (400), Expect = 1.653e-39
Identity = 121/397 (30.48%), Postives = 192/397 (48.36%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N I   E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G ++ +  ++ C   E+IV+ +  NE+ + Q+   E        D  R T  +E D    QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1288 MQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIG-SDETQVELQCRSGEQIVTVTQ-NEAGLEQIHYLE--------DRSRYTRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1661

HSP 4 Score: 145.206 bits (365), Expect = 4.346e-35
Identity = 117/406 (28.82%), Postives = 183/406 (45.07%), Query Frame = 2 
Query:  368 SQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
            S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1808 SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEA-INSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL-RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVV------EEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2193

HSP 5 Score: 144.821 bits (364), Expect = 5.708e-35
Identity = 114/390 (29.23%), Postives = 189/390 (48.46%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++  +   R  + G +  R  A NPLG+  +   + V S  +   D +H
Sbjct: 2087 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEA-VNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCR--AVNPLGEAVTTASMRVVSEASIQMDTQH 2460

HSP 6 Score: 142.51 bits (358), Expect = 2.454e-34
Identity = 104/365 (28.49%), Postives = 173/365 (47.40%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSIN 1318
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N
Sbjct: 3502 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRN 3849

HSP 7 Score: 139.813 bits (351), Expect = 2.067e-33
Identity = 107/381 (28.08%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      ++    V  + +    Q +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1252

HSP 8 Score: 139.813 bits (351), Expect = 2.067e-33
Identity = 103/390 (26.41%), Postives = 181/390 (46.41%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-------------PEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G   +   + +VT             PE      +  +E   +      P+ P    E    P F+  + +++TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F       Y+ +        + G++  R  ATN  G+  +  I+ V+
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG---TATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRFQPIYDFGYVAMNFGWVYPEDSGEYVCR--ATNLYGKDETRAIIKVS 4047

HSP 9 Score: 130.568 bits (327), Expect = 1.600e-30
Identity = 99/341 (29.03%), Postives = 159/341 (46.63%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELT 1390
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT
Sbjct: 2485 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKE------GRPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLT 2816

HSP 10 Score: 130.183 bits (326), Expect = 1.823e-30
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3261 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTAT-MKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3675

HSP 11 Score: 130.183 bits (326), Expect = 2.345e-30
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  611 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 859

HSP 12 Score: 127.487 bits (319), Expect = 1.322e-29
Identity = 101/386 (26.17%), Postives = 187/386 (48.45%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I  +  +  +     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1556 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPV-RDVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI--VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTV-YAEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1928

HSP 13 Score: 124.02 bits (310), Expect = 1.620e-28
Identity = 72/232 (31.03%), Postives = 123/232 (53.02%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQD------------ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +AP F ++L++   ++E   + L +QV P  D +L +EW K+G  L TG+R  + F+ G V L I     +DS +Y C A ++ G+  S       T   K+++  ++++ + +  D            E  P+ P+   + ++P F   L +NI   +   V  E  V+P  D T+ ++W  NGQ +   ++F +++D GYCALD+  +Y E+ GVYTC A N+ G A +S
Sbjct: 2620 QAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVS-------TSSLKIEDR-HWLQAESLHPDSLPRIGELEAPKEGRPEAPEP--TYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTS 2840

HSP 14 Score: 122.094 bits (305), Expect = 6.305e-28
Identity = 89/308 (28.90%), Postives = 141/308 (45.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSI----SQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2356 PVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQ--HVLLEAQVEPRA 2650

HSP 15 Score: 114.005 bits (284), Expect = 2.366e-25
Identity = 100/386 (25.91%), Postives = 172/386 (44.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3773

HSP 16 Score: 110.923 bits (276), Expect = 1.848e-24
Identity = 98/377 (25.99%), Postives = 165/377 (43.77%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF--IENQHVQQDENEPQYPDKHESSQS-------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVN 1480
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +  ++   +Q+   E + P   ++  S       P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + + 
Sbjct: 3128 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRI-RELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKI-ANLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKIQ 3494

HSP 17 Score: 109.383 bits (272), Expect = 7.117e-24
Identity = 91/372 (24.46%), Postives = 153/372 (41.13%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++ + +     +      P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E         A N  G+  +   L   S ++   D
Sbjct: 3029 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQADTSAPLVAE-----PPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRASLKCASKSSLILD 3364

HSP 18 Score: 107.457 bits (267), Expect = 2.600e-23
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2888 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3122

HSP 19 Score: 88.9669 bits (219), Expect = 1.159e-17
Identity = 106/433 (24.48%), Postives = 171/433 (39.49%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI---------------APENPY 1534
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV  T+  GQ+ S   LTV      A  L+ I               APE+P+
Sbjct: 4471 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRV--TSSTGQVESSAKLTVKKRRISAFQLRTIDSAEDESSSSGREDSAPESPH 4826

HSP 20 Score: 70.0922 bits (170), Expect = 7.419e-12
Identity = 93/395 (23.54%), Postives = 154/395 (38.99%), Query Frame = 2 
Query:  338 GRPKGHGSV--QSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTED------QEDDQAPSFDKGL-QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF--YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            G  K  GSV    Q   ++ + PRF+  LE +     E  + +   +  +   S S +W    +P+ +      V +     L I     +  G + C A++  G   S  ATV + P+                         ++ E  +SP F ++L   + V  G A+ L   V  +   T KV+W+ N + ++         D +G C L I   +PE++G Y CVA N  G               K VSK+N+      +++ FE     EI     + ED        D+QAP   K L + +   EG  AK  + +   P+  + W  ++E++ +    +   + DGT + L +          I   A NPLG   +  +  V  I
Sbjct: 6819 GEAKSSGSVVVSDQKPDEQIKPPRFIQPLEPKYFGEHE--VAIIEAIVESEPLS-SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAENVGGSVTST-ATVNLIPQ-------------------------EEAEEFESPRFVEELVQPVEVMDGEALLLTCQVTGKP--TPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIG---------------KSVSKTNV------KIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAVEGI 7160

HSP 21 Score: 67.781 bits (164), Expect = 3.473e-11
Identity = 93/394 (23.60%), Postives = 161/394 (40.86%), Query Frame = 2 
Query:  371 QYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDE------NEPQYPDKHES--SQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIV-SKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCT--EVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            Q E++EFE+PRF+  L   + V + + + L+ QV   G  +  VEW  + + + T  + +T+ +  +G   L I     E+ G Y CVA +K G++ S               NV     +++   E      +E    D+  S   Q+P   K L   I   +G    LE  V   P+     KV W ++ + +     +    F+ G   L I + YP+D G  +  A N  G A ++         E IV SK       +SQ+        EE+  A +  +      +PS    ++D     G  AKF I    +P   I W  NN ++ +    +         LE+ + T  + G +N +++  N +G   +     ++S +   ++
Sbjct: 6933 QEEAEEFESPRFVEELVQPVEVMDGEALLLTCQVT--GKPTPKVEWYHNAEKI-TENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-------------TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKP----KVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTT----ALFAVEGIVGSKDYRKPEWVSQM--------EEMQVALKAAK-----CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLI--NEIGMTMTRAKFDISSTSTIVEE 7287

HSP 22 Score: 56.6102 bits (135), Expect = 9.251e-8
Identity = 73/337 (21.66%), Postives = 116/337 (34.42%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +   +P           P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4419 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKGGIQP-----------PSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4698
[Close]
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332679)

HSP 1 Score: 175.637 bits (444), Expect = 6.625e-45
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3808 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDL--FDPDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4192

HSP 2 Score: 159.458 bits (402), Expect = 1.100e-39
Identity = 112/380 (29.47%), Postives = 183/380 (48.16%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S   +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V   + ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1022 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSAC-VRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPV--KTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1387

HSP 3 Score: 158.303 bits (399), Expect = 2.491e-39
Identity = 121/397 (30.48%), Postives = 192/397 (48.36%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N I   E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G ++ +  ++ C   E+IV+ +  NE+ + Q+   E        D  R T  +E D    QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1288 MQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIG-SDETQVELQCRSGEQIVTVTQ-NEAGLEQIHYLE--------DRSRYTRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1661

HSP 4 Score: 144.436 bits (363), Expect = 5.867e-35
Identity = 117/406 (28.82%), Postives = 183/406 (45.07%), Query Frame = 2 
Query:  368 SQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
            S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1808 SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEA-INSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL-RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVV------EEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2193

HSP 5 Score: 144.436 bits (363), Expect = 6.694e-35
Identity = 114/390 (29.23%), Postives = 189/390 (48.46%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++  +   R  + G +  R  A NPLG+  +   + V S  +   D +H
Sbjct: 2087 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEA-VNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCR--AVNPLGEAVTTASMRVVSEASIQMDTQH 2460

HSP 6 Score: 142.124 bits (357), Expect = 3.369e-34
Identity = 104/365 (28.49%), Postives = 173/365 (47.40%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSIN 1318
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N
Sbjct: 3502 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRN 3849

HSP 7 Score: 139.428 bits (350), Expect = 2.787e-33
Identity = 107/381 (28.08%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      ++    V  + +    Q +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1252

HSP 8 Score: 139.428 bits (350), Expect = 2.836e-33
Identity = 103/390 (26.41%), Postives = 181/390 (46.41%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-------------PEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G   +   + +VT             PE      +  +E   +      P+ P    E    P F+  + +++TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F       Y+ +        + G++  R  ATN  G+  +  I+ V+
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG---TATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRFQPIYDFGYVAMNFGWVYPEDSGEYVCR--ATNLYGKDETRAIIKVS 4047

HSP 9 Score: 130.568 bits (327), Expect = 1.672e-30
Identity = 106/382 (27.75%), Postives = 168/382 (43.98%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT             ATN  G   +   L          D +H
Sbjct: 2485 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKE------GRPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQH 2857

HSP 10 Score: 130.183 bits (326), Expect = 1.906e-30
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3261 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTAT-MKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3675

HSP 11 Score: 129.798 bits (325), Expect = 2.722e-30
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  611 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 859

HSP 12 Score: 127.102 bits (318), Expect = 1.856e-29
Identity = 101/386 (26.17%), Postives = 187/386 (48.45%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I  +  +  +     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1556 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPV-RDVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI--VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTV-YAEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1928

HSP 13 Score: 122.094 bits (305), Expect = 7.503e-28
Identity = 89/308 (28.90%), Postives = 141/308 (45.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSI----SQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2356 PVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQ--HVLLEAQVEPRA 2650

HSP 14 Score: 113.62 bits (283), Expect = 2.694e-25
Identity = 100/386 (25.91%), Postives = 172/386 (44.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3773

HSP 15 Score: 110.923 bits (276), Expect = 2.140e-24
Identity = 98/376 (26.06%), Postives = 163/376 (43.35%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQ-HVQQDENEPQYPDKHES-------SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVN 1480
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +   Q    Q   E + P   ++       ++ P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + + 
Sbjct: 3128 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKI-ANLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKIQ 3494

HSP 16 Score: 109.383 bits (272), Expect = 7.002e-24
Identity = 91/372 (24.46%), Postives = 153/372 (41.13%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++ + +     +      P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E         A N  G+  +   L   S ++   D
Sbjct: 3029 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQADTSAPLVAE-----PPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRASLKCASKSSLILD 3364

HSP 17 Score: 107.457 bits (267), Expect = 2.625e-23
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2888 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3122

HSP 18 Score: 88.5817 bits (218), Expect = 1.589e-17
Identity = 101/412 (24.51%), Postives = 165/412 (40.05%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI 1516
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV ++   GQ+ S   LTV      A  L+ I
Sbjct: 4471 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSST--GQVESSAKLTVKKRRISAFQLRTI 4805

HSP 19 Score: 70.4774 bits (171), Expect = 6.159e-12
Identity = 93/395 (23.54%), Postives = 154/395 (38.99%), Query Frame = 2 
Query:  338 GRPKGHGSV--QSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTED------QEDDQAPSFDKGL-QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF--YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            G  K  GSV    Q   ++ + PRF+  LE +     E  + +   +  +   S S +W    +P+ +      V +     L I     +  G + C A++  G   S  ATV + P+                         ++ E  +SP F ++L   + V  G A+ L   V  +   T KV+W+ N + ++         D +G C L I   +PE++G Y CVA N  G               K VSK+N+      +++ FE     EI     + ED        D+QAP   K L + +   EG  AK  + +   P+  + W  ++E++ +    +   + DGT + L +          I   A NPLG   +  +  V  I
Sbjct: 6819 GEAKSSGSVVVSDQKPDEQIKPPRFIQPLEPKYFGEHE--VAIIEAIVESEPLS-SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAENVGGSVTST-ATVNLIPQ-------------------------EEAEEFESPRFVEELVQPVEVMDGEALLLTCQVTGKP--TPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIG---------------KSVSKTNV------KIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAVEGI 7160

HSP 20 Score: 67.3958 bits (163), Expect = 4.519e-11
Identity = 93/394 (23.60%), Postives = 161/394 (40.86%), Query Frame = 2 
Query:  371 QYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDE------NEPQYPDKHES--SQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIV-SKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCT--EVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            Q E++EFE+PRF+  L   + V + + + L+ QV   G  +  VEW  + + + T  + +T+ +  +G   L I     E+ G Y CVA +K G++ S               NV     +++   E      +E    D+  S   Q+P   K L   I   +G    LE  V   P+     KV W ++ + +     +    F+ G   L I + YP+D G  +  A N  G A ++         E IV SK       +SQ+        EE+  A +  +      +PS    ++D     G  AKF I    +P   I W  NN ++ +    +         LE+ + T  + G +N +++  N +G   +     ++S +   ++
Sbjct: 6933 QEEAEEFESPRFVEELVQPVEVMDGEALLLTCQVT--GKPTPKVEWYHNAEKI-TENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-------------TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKP----KVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTT----ALFAVEGIVGSKDYRKPEWVSQM--------EEMQVALKAAK-----CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLI--NEIGMTMTRAKFDISSTSTIVEE 7287

HSP 21 Score: 56.6102 bits (135), Expect = 8.947e-8
Identity = 73/337 (21.66%), Postives = 116/337 (34.42%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +   +P           P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4419 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKGGIQP-----------PSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4698
[Close]
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.2 (titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030113-2])

HSP 1 Score: 111.694 bits (278), Expect = 1.854e-24
Identity = 105/418 (25.12%), Postives = 179/418 (42.82%), Query Frame = 2 
Query:  359 SVQSQYESKE-FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTV---FERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-------------PATVRVTPEAKV---DNNVNFIENQHVQQDENEPQYPDKH--ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI----APENP 1531
            S+    E+KE    P+F+  L+N I+V E   + L+  V   G+    + WLK+   +++  ++  +     +G     I  +   DS  Y   A +K+G++ +              P    + P+      D     IE  H++  + + +  D +  E  Q P+F K L S + + + G VH E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V  S L E    Q       I  E   +    +D  +   P      + + + EG  AK+   +  +P   + W +N   +      + + DG I+YLE+T C      ++RV+A NP G       L +    ++   L+      APE P
Sbjct:  965 SMTLTVEAKENLVRPQFIEKLKN-ISVKEGSLVELA--VKAIGNPLPDIVWLKNSD-IISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAGRDTTRCRVNVEVEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEKQQKPHFKKKLTS-VRMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFGADQTSATLI--VKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTGDDMSEKSKPEIVLLPEPIRVFEGETAKYRCRVTGYPTPKVNWYLNGLLIRKSKRFRLHYDG-IHYLEITDCKSYDSGDVRVLAENPEGTAEHTVKLEIQQKEDFRTILRRAPELKAPEAP 1374

HSP 2 Score: 67.3958 bits (163), Expect = 6.632e-11
Identity = 60/232 (25.86%), Postives = 102/232 (43.97%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESS------QSPYFSKDLESNITVNQGGAVHLETIV-DPRNDVTLKVDWHKNGQSMSLGSRFNAVF--DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  ++ L+N +TV E + + L  Q++  G  + ++ W ++   +     F   +E+ Y  L I  +  EDSG + C A S++G  + S    V+V+ E +    +   E Q    +  +    +    S       +P+F K       V  G  V +  I   P+  +     W K+G  ++ G R+      D G C L+I  T+ +D G YT  AKN  GE  +S S +
Sbjct:  392 PTLIAGLKN-VTVTEGESVTLECQIS--GHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCYLLVKVSEEIESREEITVQEKQRTVVEAKDETMSEISSVSVDTGAPAAPFFVKKPVVQKLVEGGSVVFVCQIGGSPKPHIY----WKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGETSASTSLL 616

HSP 3 Score: 53.5286 bits (127), Expect = 1.173e-6
Identity = 52/235 (22.13%), Postives = 96/235 (40.85%), Query Frame = 2 
Query:  887 KVDWHKNGQSMSLGSRFNA-VFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS--------------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQ--APSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFY--GDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN-----YAQDLKHIA 1519
            K+ W+K+G+ +  G R+   V   G  +L +    PED+G+YT  A N  G A SS              + Q   +   +  S  +   +S S  +    ++         +T++ + ++   P F +        EG  A+F + +   P     W  + ++V++D  +K     DGT + + +    +       VVA N  G+      LTV +  N     + + LK+I+
Sbjct:  756 KIAWYKDGKRIKHGGRYQMEVLQDGRASLRLPVVLPEDEGIYTAFASNMKGNAVSSGKLYVESTAAAQPYYPQAEAVRRVQSTSPMSARSASYSPGRSPARSVSRSPARRLDDTDESQLERLYKPVFVQKPSSFRCSEGQTARFDLKVVGRPMPDTFWFHSGQQVVNDYTHKIVVKEDGTQSMIVVPAMPQ-DSGEWTVVAQNRAGKTTVSMTLTVEAKENLVRPQFIEKLKNIS 989

HSP 4 Score: 53.1434 bits (126), Expect = 1.474e-6
Identity = 55/221 (24.89%), Postives = 90/221 (40.72%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFN-AVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            E P+ + +++  ++V    P R S +V   G     V W K+ + L +G +   + E     L ++    ED+  Y C AK+  G   S+ +     PE              V  D   P  P        P     ++ N + N+G +   +  V   +   L + W+   + +     F  + FD   C L+I   YPED+G YTCVA+N+ G    S
Sbjct: 2662 EPPQIIRHMQ-PLSVEAGKPARFSVEVT--GIPQPQVSWYKNSQALSSGFKCKFLREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSASLNVEVPEV-------------VSPDTGAPLSP--------PVVLTPIQ-NTSANEGQSARFQCRVSGED---LTITWYCKDKEIKQSDIFRVSQFDEN-CQLEITRVYPEDEGEYTCVARNSAGMVSCS 2853
[Close]
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.2 (titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030113-2])

HSP 1 Score: 109.768 bits (273), Expect = 5.613e-24
Identity = 101/404 (25.00%), Postives = 173/404 (42.82%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTV---FERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-------------PATVRVTPEAKV---DNNVNFIENQHVQQDENEPQYPDKH--ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI----APENP 1531
            P+F+  L+N I+V E   + L+  V   G+    + WLK+   +++  ++  +     +G     I  +   DS  Y   A +K+G++ +              P    + P+      D     IE  H++  + + +  D +  E  Q P+F K L S + + + G VH E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V  S L E    Q       I  E   +    +D  +   P      + + + EG  AK+   +  +P   + W +N   +      + + DG I+YLE+T C      ++RV+A NP G       L +    ++   L+      APE P
Sbjct:  935 PQFIEKLKN-ISVKEGSLVELA--VKAIGNPLPDIVWLKNSD-IISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAGRDTTRCRVNVEVEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEKQQKPHFKKKLTS-VRMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFGADQTSATLI--VKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTGDDMSEKSKPEIVLLPEPIRVFEGETAKYRCRVTGYPTPKVNWYLNGLLIRKSKRFRLHYDG-IHYLEITDCKSYDSGDVRVLAENPEGTAEHTVKLEIQQKEDFRTILRRAPELKAPEAP 1330

HSP 2 Score: 75.8702 bits (185), Expect = 1.756e-13
Identity = 69/236 (29.24%), Postives = 100/236 (42.37%), Query Frame = 2 
Query:  374 YESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-DENEPQYPDKHESSQSPYFSKDLESN-----------ITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAE 1045
            Y+ ++ + P F   L   + +    P+    ++ P GD ++ VEWL DGKPL    R   + E GY  LD   +   DSGV  C A +K G +Q++ AT+ V  E  +  +    E + VQ+ DE E      HE   S     D+              I V +G        V      T KV+W+ NG  +    RF   +D G   L+I      D G    +A+N  G AE
Sbjct: 1074 YDKEKQQKPHFKKKL-TSVRMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFGADQTS-ATLIVKDEKSLVEDSQLPEGRKVQRMDEME---RIAHEGGPSGVTGDDMSEKSKPEIVLLPEPIRVFEGETAKYRCRVT--GYPTPKVNWYLNGLLIRKSKRFRLHYD-GIHYLEITDCKSYDSGDVRVLAENPEGTAE 1301

HSP 3 Score: 71.2478 bits (173), Expect = 5.069e-12
Identity = 62/237 (26.16%), Postives = 91/237 (38.40%), Query Frame = 2 
Query:  356 GSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT 1066
            G+     E +  + P F+ + E+  TV   +P+RL  QV    D  +S+ W++DGK L T       FE     LDI  S  +D+G Y C A +++G +  + +     P                                  P F K LE  I   QG +  L   V  +    L V W  N + ++   +    F      L+I+     D G YTC   N  G  ES  SQ+T
Sbjct: 4266 GTATCCTELRVVDKPSFVKSFES-TTVAVGNPLRLECQVDE--DTGVSIIWMRDGKKLHTTMDCKLAFEEKIACLDIQKSKLKDTGTYTCTAANEAGSSSCSSSVTVQEP----------------------------------PVFVKRLEPKILWKQGMSSRLSCTV--KGSPELHVTWFLNDKPLNSSEKHKITFKTNQATLEIIDLSESDSGNYTCEVMNEAG-CESCSSQVT 4462

HSP 4 Score: 65.0846 bits (157), Expect = 3.350e-10
Identity = 57/215 (26.51%), Postives = 95/215 (44.19%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIV-DPRNDVTLKVDWHKNGQSMSLGSRFNAVF--DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            +TV E + + L  Q++  G  + ++ W ++   +     F   +E+ Y  L I  +  EDSG + C A S++G   +   +  +  +AK D  ++ I +  V  D   P          +P+F K       V  G  V +  I   P+  +     W K+G  ++ G R+      D G C L+I  T+ +D G YT  AKN  GE  +S S +
Sbjct:  379 VTVTEGESVTLECQIS--GHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAG---TISTSCYLLVKAK-DETMSEISS--VSVDTGAPA---------APFFVKKPVVQKLVEGGSVVFVCQIGGSPKPHIY----WKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGETSASTSLL 572

HSP 5 Score: 64.6994 bits (156), Expect = 4.783e-10
Identity = 94/408 (23.04%), Postives = 153/408 (37.50%), Query Frame = 2 
Query:  284 NLPNGNIVNA----QGNHVASFGRPKGHGSVQSQYESKEFE-APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISD----SVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            N P+  I+ A     G +        G  S  ++   KE +  P F       I   E   +++  +VA  G   L+V W KDG+ + T   +   F+    VL I  S   DSG YVC A +++G   S   +V +T              Q V    + P  P                  +TVN+G  + L   V  R    LK+ W K+ + +S  +     F  G   L++      D G Y C A N  G +E   S++T        + +    S   ++KKF+N    E+                      + V I E   A FI  +   P  ++ W       ++     S+ +   +  +   E+T+ ++ GQ+  R VA+N  G++     L V+
Sbjct: 6880 NTPHLTILKADKADSGRYSCHASNDVGKDSCSTEVSVKERKIPPSFTKKPSTTIEDIEGKVVKIEGRVA--GSQPLTVNWYKDGREIFTSDFYDVTFKSSLAVLCIKKSQLSDSGTYVCKATNEAG-TASFEVSVHITA-------------QKVPPSFDLPLKP------------------VTVNEGETLTLSCHV--RGSPPLKIQWMKDRRELSSSANTKITFVDGTATLEMTRVSKTDSGDYLCKATNEAG-SEFCKSKVTIKDKPGAPAPAQAAPSPPQEVKKFDNLFFIEVP---------------------KSVHIVEKGTATFIAKVGGDPIPNVKWMKGKWRQMTHGGRISIEQKGQEAKMEIKEVTK-SDSGQY--RCVASNKHGEIECSTDLNVD 7226

HSP 6 Score: 62.003 bits (149), Expect = 2.789e-9
Identity = 81/382 (21.20%), Postives = 148/382 (38.74%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFN-AVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS----------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQ-EEINDAFRNTEDQE--------DDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTV 1477
            E P+ + +++  ++V    P R S +V   G     V W K+ + L +G +   + E     L ++    ED+  Y C AK+  G   S+ +     PE              V  D   P  P        P     ++ N + N+G +   +  V   +   L + W+   + +     F  + FD   C L+I   YPED+G YTCVA+N+ G    S            +I    +E++   S+L   +  + + +  ++   E +       +Q+          + P F+  L    +  G + +F + +   P+  + W  N + + S S+  F  +     L +T+  +  +      A+N  GQ     IL V
Sbjct: 2618 EPPQIIRHMQ-PLSVEAGKPARFSVEVT--GIPQPQVSWYKNSQALSSGFKCKFLREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSASLNVEVPEV-------------VSPDTGAPLSP--------PVVLTPIQ-NTSANEGQSARFQCRVSGED---LTITWYCKDKEIKQSDIFRVSQFDEN-CQLEITRVYPEDEGEYTCVARNSAGMVSCSALLKVDVTRVREEIEAKIEEEVKEFSSLTVKTEEEEETYSTSLHLPEKSKTLELKPEQKRFSSSLERKREKPVFESKLTPAEVTIGESVRFTVTVSGFPKPKVQWFHNGKAITSSSIYTFVEERDEYSLIITKVKKDYEGEYSCTASNRFGQTTCKTILKV 2970

HSP 7 Score: 58.5362 bits (140), Expect = 3.174e-8
Identity = 55/200 (27.50%), Postives = 83/200 (41.50%), Query Frame = 2 
Query:  440 EEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE 1039
            E  P++   +VA  G + + V WLKDG+ L + + ++  F+    VL+I       SG Y CVA +  G      A+ R    AK+                 EP+YP        P F K L   + V+ G  V LE  +     + +KV W K+ + +  G  +          L IL     D G Y+C A N+ G+
Sbjct: 6744 EGQPVQFECRVA--GSSPMEVSWLKDGEALRSDSEYTMSFDDNSAVLNIAKGEMRHSGEYTCVATNSVGS-----ASCR----AKLTL--------------QEPRYP--------PVFDKKL-VPVDVSVGDTVELECHMT--GSMPIKVTWSKDHKDIRTGGNYKISCVENTPHLTILKADKADSGRYSCHASNDVGK 6907

HSP 8 Score: 51.2174 bits (121), Expect = 5.324e-6
Identity = 52/235 (22.13%), Postives = 96/235 (40.85%), Query Frame = 2 
Query:  887 KVDWHKNGQSMSLGSRFNA-VFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS--------------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQ--APSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFY--GDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN-----YAQDLKHIA 1519
            K+ W+K+G+ +  G R+   V   G  +L +    PED+G+YT  A N  G A SS              + Q   +   +  S  +   +S S  +    ++         +T++ + ++   P F +        EG  A+F + +   P     W  + ++V++D  +K     DGT + + +    +       VVA N  G+      LTV +  N     + + LK+I+
Sbjct:  712 KIAWYKDGKRIKHGGRYQMEVLQDGRASLRLPVVLPEDEGIYTAFASNMKGNAVSSGKLYVESTAAAQPYYPQAEAVRRVQSTSPMSARSASYSPGRSPARSVSRSPARRLDDTDESQLERLYKPVFVQKPSSFRCSEGQTARFDLKVVGRPMPDTFWFHSGQQVVNDYTHKIVVKEDGTQSMIVVPAMPQ-DSGEWTVVAQNRAGKTTVSMTLTVEAKENLVRPQFIEKLKNIS 945

HSP 9 Score: 51.2174 bits (121), Expect = 5.461e-6
Identity = 73/364 (20.05%), Postives = 121/364 (33.24%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            EA  F+  L+N   ++ E    L  +   AG   + V+W KDG+ + +  +++        +L+ L S +E +G Y C   + +G +    A   + P                                  P+F + LE  + V  G AV L+  +    ++  K+ W K    +         F +G   L +      D G YTC A+N+ G A SS H  +                                          Q+    PSF K +  +   EG   +F   +       + W  + E + SDS      D     L + +           VATN +G  +    LT+ 
Sbjct: 6540 EAAHFVKKLKN---LSVEMGKSLILECTYAGSPKILVKWHKDGQEIYSSYKYNITTTESSCILECLNSDKEAAGKYTCEVSNDAGHDICEAAVSILEP----------------------------------PFFIESLEP-MEVTAGDAVCLKCQIGGTPEI--KISWFKADGKVRSSPTCKMEFFKGIACLKLAKVAKSDIGEYTCKAENSIGSATSSCHLTV------------------------------------------QDVKTPPSFPKKITSIMQIEGQPVQFECRVAGSSPMEVSWLKDGEALRSDSEYTMSFDDNSAVLNIAKGEMRHSGEYTCVATNSVGSASCRAKLTLQ 6821

HSP 10 Score: 51.2174 bits (121), Expect = 5.697e-6
Identity = 58/252 (23.02%), Postives = 93/252 (36.90%), Query Frame = 2 
Query:  311 AQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT 1066
            A G +        GH   ++       E P F+ +LE  + V   D + L  Q+   G   + + W K    + +       F +G   L +    + D G Y C A++  G   S  ++  +T                VQ  +  P +P K  S             I   +G  V  E  V       ++V W K+G+++   S +   FD     L+I        G YTCVA N+ G A S  +++T
Sbjct: 6608 AAGKYTCEVSNDAGHDICEAAVSI--LEPPFFIESLE-PMEVTAGDAVCLKCQIG--GTPEIKISWFKADGKVRSSPTCKMEFFKGIACLKLAKVAKSDIGEYTCKAENSIG---SATSSCHLT----------------VQDVKTPPSFPKKITS-------------IMQIEGQPVQFECRV--AGSSPMEVSWLKDGEALRSDSEYTMSFDDNSAVLNIAKGEMRHSGEYTCVATNSVGSA-SCRAKLT 6819
[Close]
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.1 (titin, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030616-413])

HSP 1 Score: 99.3673 bits (246), Expect = 9.538e-21
Identity = 101/385 (26.23%), Postives = 161/385 (41.82%), Query Frame = 2 
Query:  359 SVQSQYESKE-FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPAT------VRVTPEAKVDNNVNFIENQHVQQDENEP--------QYPD----KHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLG 1447
            S+    E+KE    P+F+  L N I+V E   + L+  V   G+    + WLK+   +          E  +G     I  S   DS  Y   A +K+G++ +          V+  PE K+       + + +   E EP        Q+ D      E  Q P+F K L S + + + G  H E  + P  D T+ V+W  +G+ ++  +R   V + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V ++ L E    Q       I  E   +    +D  +   P      + V + EG  AKF   +  +P   + W +N   +      +   DG I+YLE+         +++V A NP G
Sbjct:  967 SMTLTVEAKENLVRPQFVEKLRN-ISVKEGTLVELA--VKAIGNPLPDIVWLKNSDIISPHKHPHIRIEGGKGEANFKIPSSAGSDSAWYTATAINKAGRDTTRCRVNVEVDAVKPQPEKKLVIPKGTYKAKEIAAPELEPLHLRYGQEQWEDGDLYDKEKQQKPHFKKKLTS-VRLKRFGPAHFECRLTPIGDPTMIVEWLHDGKPLAAANRLRMVNEFGYCSLDYEAAYSRDSGVITCRATNKFGVDQTSATLI--VKDEKSLVEETQLPEGRRGQRIDEIERIAHEGGPSGVTGDDNLEKTKPEIVLLPEPVRVMEGETAKFRCRVTGYPTPKVNWYLNGLLIRKSKRFRLQFDG-IHYLEIVDTKSYDSGDVKVSAENPEG 1344

HSP 2 Score: 73.559 bits (179), Expect = 8.066e-13
Identity = 70/236 (29.66%), Postives = 97/236 (41.10%), Query Frame = 2 
Query:  374 YESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-DENEPQYPDKHESSQSPYFSKD-----------LESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAE 1045
            Y+ ++ + P F   L   + +    P     ++ P GD ++ VEWL DGKPL    R   V E GY  LD   +   DSGV  C A +K G +Q++ AT+ V  E  +       E +  Q+ DE E      HE   S     D           L   + V +G        V      T KV+W+ NG  +    RF   FD G   L+I+ T   D G     A+N  G  E
Sbjct: 1120 YDKEKQQKPHFKKKL-TSVRLKRFGPAHFECRLTPIGDPTMIVEWLHDGKPLAAANRLRMVNEFGYCSLDYEAAYSRDSGVITCRATNKFGVDQTS-ATLIVKDEKSLVEETQLPEGRRGQRIDEIERI---AHEGGPSGVTGDDNLEKTKPEIVLLPEPVRVMEGETAKFRCRVT--GYPTPKVNWYLNGLLIRKSKRFRLQFD-GIHYLEIVDTKSYDSGDVKVSAENPEGVTE 1347

HSP 3 Score: 66.6254 bits (161), Expect = 1.212e-10
Identity = 83/397 (20.91%), Postives = 155/397 (39.04%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQ-----------------YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVF--DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS--------------------HSQITCIPDE-----------------------KIVSKSNLNESSIS----QLKKFENNIQEEINDAFRN-------TEDQE---------DDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVI 1333
            P  ++ L+N +TV E + + L  Q++  G  + ++ W ++   +     F   +E+ Y  L I  +  EDSG + C A S++G  + S    V+V+ E +   + + +  +HV+    + Q                  P +  +  +P+F K       V +G  V  +  V   P+  +     W K+G  ++ G R+   +  D G C L+I  T+ +D G YT  A+N  GE+ +S                     +++  I +E                       ++  ++ + +S IS    Q+   E    +E+             TED E         D  +P F+  ++   + EG +A F   +   P   IVW  + + +I
Sbjct:  393 PTIVAALKN-VTVTEGETVALECQIS--GYPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCYLLVKVSEEIESREDFSNVP-EHVEMSAQQRQGAIEAKEETMSEISAVSEPVEAGAGAAPFFVKKPTVQKLV-EGDRVVFDCQVGGTPKPHIY----WKKSGIPLTTGYRYRVAYNKDTGACKLEISMTFADDAGEYTVFARNPLGESSASAILLDEESYDQYVKKQEDHFKTEVPTIAEEPKVADVPAAFETVTEKDQLLMQRRMAQQTQMMQSFISEQELQMSSLEQRFIQEVEFHLLKITYQELVTEDGEQLDMSVPEHDAVSPVFNTPVKGYRLREGMSATFHCRMSGTPLPKIVWYKDGKRII 780

HSP 4 Score: 62.7734 bits (151), Expect = 1.936e-9
Identity = 77/350 (22.00%), Postives = 141/350 (40.29%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-------HSQITCIP---DEKIVSKSNLNESSISQLKKFENNIQEEIN-------DAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINYLEL 1387
            EAP+ + +++  ++V    P R S +V   G     V W K+ + L +G +     E     L ++    ED+  Y C AK+  G   S+ A++ V                    + +E   P+      +P     L+ + +V +G +   +  V   +   LK+ W++  + + +   F        C LDI   Y  D G YTCVA+N+ G    S       HS  T        ++V+   + E+  S ++ FE+     ++       +  + T ++E   +P     + DV +  G+ A+F  +    P   IVW  N   +  ++ V   +  G I+ + L
Sbjct: 2665 EAPQIIRHMQ-PLSVEAGKPARFSVEVT--GIPQPQVSWYKNSQALSSGFKCKFQREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSS-ASLNV--------------------EVSEVVSPETVAPVSAPVIITSLQ-DTSVEEGESARFQCRVSGDD---LKITWYRRDKEIVMSDNFQMTQFGDTCQLDISRAYVADAGEYTCVARNSGGMVSCSAVLTVNDHSVSTVASVSTHSEMVTSQTMTETRSSTMESFESVSTVMMSSTSVKSIEVQQVTSEEEYSTSPVLTLKMSDVIVSLGDVAQFNCSFSGQPFTEIVWDHNGRTITNTERVQYAHEAGLISLIIL 2986

HSP 5 Score: 61.2326 bits (147), Expect = 5.627e-9
Identity = 87/390 (22.31%), Postives = 142/390 (36.41%), Query Frame = 2 
Query:  326 VASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRV---TPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            + S  R K    V+S     +   P F + L++ I+ + +  ++L+ +++  GD   S+ W+KDGK L  G ++    E G   L+I  +   DSGVY C A++  G   +N  T+ +   T  A ++      E   + + EN       +E  QS    K  ++ +T++QG  V L   +   +D    V W  NG  +S    +          L I      D G+ TC       EA +    + C                                  F  T       +PSF        ++EG    F   I   P   + W  +N  +   S  K      +  LE+   T        V A N  GQ ++   L V ++
Sbjct: 28627 IKSPTRVKSPPMVKSPTPKDKTSKPSFSTELKD-ISASADTIVKLTVKLS--GDLKPSISWMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVGSVSTN-CTLTIQARTTAAALEGKSELTE--EIVKKEN------TYEEVQSYTEIKASKTQMTISQGQTVTLRASIPEASD----VKWILNGAELSNSESYRYGVSGSDHTLTIKSISHHDQGILTC-------EARTEQGVVKC---------------------------------QFDMTVSATHSGSPSFLVQPHSQNVNEGQNVTFTCEITGEPSPEVEWLKDNAVISITSNMKLSRSKNVYTLEIHNATIEDSGKFTVKAKNKFGQCSATASLNVLTL 28960

HSP 6 Score: 53.9138 bits (128), Expect = 9.831e-7
Identity = 90/420 (21.43%), Postives = 154/420 (36.67%), Query Frame = 2 
Query:  302 IVNAQGNHVASFGRPKGHGSVQSQ-------YESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSP------------YFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSL---GSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNI-RVVATNPLGQMASDCILTVNSINN 1492
            +V  + N V S  R    GS++SQ        +   FE       +++ I  NE D I+L   +    D++  V W    + L  G +    +E G   + I      D G Y C   ++ G++ +         E  V+   ++ E    +  +   +  D     Q P            Y  +D+   +T+     VH E    PR      V W KNGQ +       ++  V D+G   L I    P+DD  Y+ VA+N  G+ +S  +++T +P                +    +N ++                  P F + L ++   EG + +F + +  HP  S+ W  +   +      +   +G   Y+   R T      I RV ATN  G  +    L V  + +
Sbjct: 27466 MVKKEWNTVVSSARVASGGSIRSQRGVTISKVKIAPFEHGPVAGQIKHSI-ANEGDDIKLICNID-NYDSTTEVTWYCGVRQLEEGAKCEITYEDGLATITIKGITRTDDGTYRCKVVNEYGEDSAY-------AELFVEGVRSYREFYTARVVKKVKRRVDTARLLQRPPEFTLPLCNRTAYIGEDVRFGVTIT----VHPE----PR------VIWLKNGQKIMPDDDDKKYTLVSDKGLYQLVIRKLDPDDDAEYSVVARNRFGD-DSCKARLTVVP----------------RPSPADNTLR------------------PMFKRLLANLECKEGQSVRFEMRVSGHP--SLKWEKDGMPLAFGPQIEVVHEGLDYYVLHVRGTLPEDSGIYRVTATNTAGSASCQATLKVERVTH 27825

HSP 7 Score: 51.6026 bits (122), Expect = 4.072e-6
Identity = 51/238 (21.43%), Postives = 100/238 (42.02%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDG-------KPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQS-----------------NPATVRVTPEAKVDNNVNFIENQHVQQDE---------NEP--QYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            +TV+E + ++ +  V   G+ + SV W+++        + L+T T + + FE   V +        D G Y  V ++  G+ ++                 +P  V+     ++ +       Q ++  E         + P  + P   + +  P FS +L+ +I+ +    V L   V    D+   + W K+G+++S G ++    + G   L+I      D GVYTC A+N+ G
Sbjct: 28505 LTVSEGESVKFTCDVD--GEPAPSVTWMREAQIIVSSQRHLITSTEYKSTFEINKVEMS-------DGGNYTVVVENSEGKQEAQFSLTIRKPTPVQKVVASPPRVKSPESPRIKSPFGIRSPQRIKSPEPIKSPTRVKSPPMVKSPTPKDKTSKPSFSTELK-DISASADTIVKL--TVKLSGDLKPSISWMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVG 28730
[Close]
BLAST of Titin vs. Ensembl Zebrafish
Match: obsl1b (obscurin like cytoskeletal adaptor 1b [Source:ZFIN;Acc:ZDB-GENE-050809-29])

HSP 1 Score: 67.0106 bits (162), Expect = 7.009e-11
Identity = 96/397 (24.18%), Postives = 166/397 (41.81%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIE-EDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMS---LGSRFNAVF-DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT-CIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTV-----------NSINNYAQDLKHIAPENP 1531
            APRFL+     +  +  D + L  Q+   GD   +V W ++ + +    R+  VFE G V   I+ S+  EDSG Y+C AK+  G+  +  AT++V  EA+          +   ++EN+P+           +  K L + +   +      +   +PR +V     W K+G+ ++     + F+  + D G+  L I  T   D GVYTC A+N  GE+ +    +    P  +   + N N  +    K  +   +     A R     +DD  P+  K ++   + EG  AKF   +   P+  I+W  + + ++S      Y D    + L++  C +         A+N  GQ  S   L V           N +  + +DL  +  E P
Sbjct:    7 APRFLAYPRPVVVQSGTDAV-LKCQIG--GDPRPAVIWERNNEKIHPEGRYR-VFEDGNVYNLIITSVTMEDSGQYICKAKNCIGETYA-AATLKVEGEAQ----------EMEVREENKPR-----------FLIKPLSTRVGRGEDAMFSCKLWGNPRPEVM----WEKDGKKLNEIFESTHFSISYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHE--DEGNRNGYTNGHWKAHQGKQRSGRQVATR----LKDDPLPNSAK-VKMFAVTEGKHAKFRCYVTGKPKPEILWRKDGKLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCSASNTAGQTLSAVHLIVKEPPVRFKQPLNDLQVWERDLAVLECEVP 366

HSP 2 Score: 63.929 bits (154), Expect = 7.512e-10
Identity = 72/274 (26.28%), Postives = 114/274 (41.61%), Query Frame = 2 
Query:  260 TNMNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPL---LTGTRFSTVFERG-YVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPAT-VRVTPEAKVDNNVNFIENQHVQQDENEP----QYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            T++  EDS        N  G   A+    K  G  Q + E +E   PRFL    +      ED +  S ++   G+    V W KDGK L      T FS  ++ G +  L I  +   D GVY C A+++ G++ +     V   P  + + N N   N H +  + +     Q   + +    P  +K     +T  +           P+ ++     W K+G+ +  G R+    DR GY  L +LY   +D+GVY C A N  G+  S+
Sbjct:   68 TSVTMEDSGQYICKAKNCIGETYAA-ATLKVEGEAQ-EMEVREENKPRFLIKPLSTRVGRGEDAM-FSCKLW--GNPRPEVMWEKDGKKLNEIFESTHFSISYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHEDEGNRNGYTNGHWKAHQGKQRSGRQVATRLKDDPLPNSAKVKMFAVTEGKHAKFRCYVTGKPKPEIL----WRKDGKLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCSASNTAGQTLSA 332
[Close]
BLAST of Titin vs. Ensembl Zebrafish
Match: obsl1b (obscurin like cytoskeletal adaptor 1b [Source:ZFIN;Acc:ZDB-GENE-050809-29])

HSP 1 Score: 67.0106 bits (162), Expect = 7.262e-11
Identity = 96/397 (24.18%), Postives = 166/397 (41.81%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIE-EDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMS---LGSRFNAVF-DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT-CIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTV-----------NSINNYAQDLKHIAPENP 1531
            APRFL+     +  +  D + L  Q+   GD   +V W ++ + +    R+  VFE G V   I+ S+  EDSG Y+C AK+  G+  +  AT++V  EA+          +   ++EN+P+           +  K L + +   +      +   +PR +V     W K+G+ ++     + F+  + D G+  L I  T   D GVYTC A+N  GE+ +    +    P  +   + N N  +    K  +   +     A R     +DD  P+  K ++   + EG  AKF   +   P+  I+W  + + ++S      Y D    + L++  C +         A+N  GQ  S   L V           N +  + +DL  +  E P
Sbjct:    7 APRFLAYPRPVVVQSGTDAV-LKCQIG--GDPRPAVIWERNNEKIHPEGRYR-VFEDGNVYNLIITSVTMEDSGQYICKAKNCIGETYA-AATLKVEGEAQ----------EMEVREENKPR-----------FLIKPLSTRVGRGEDAMFSCKLWGNPRPEVM----WEKDGKKLNEIFESTHFSISYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHE--DEGNRNGYTNGHWKAHQGKQRSGRQVATR----LKDDPLPNSAK-VKMFAVTEGKHAKFRCYVTGKPKPEILWRKDGKLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCSASNTAGQTLSAVHLIVKEPPVRFKQPLNDLQVWERDLAVLECEVP 366

HSP 2 Score: 63.929 bits (154), Expect = 7.028e-10
Identity = 72/274 (26.28%), Postives = 114/274 (41.61%), Query Frame = 2 
Query:  260 TNMNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPL---LTGTRFSTVFERG-YVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPAT-VRVTPEAKVDNNVNFIENQHVQQDENEP----QYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            T++  EDS        N  G   A+    K  G  Q + E +E   PRFL    +      ED +  S ++   G+    V W KDGK L      T FS  ++ G +  L I  +   D GVY C A+++ G++ +     V   P  + + N N   N H +  + +     Q   + +    P  +K     +T  +           P+ ++     W K+G+ +  G R+    DR GY  L +LY   +D+GVY C A N  G+  S+
Sbjct:   68 TSVTMEDSGQYICKAKNCIGETYAA-ATLKVEGEAQ-EMEVREENKPRFLIKPLSTRVGRGEDAM-FSCKLW--GNPRPEVMWEKDGKKLNEIFESTHFSISYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHEDEGNRNGYTNGHWKAHQGKQRSGRQVATRLKDDPLPNSAKVKMFAVTEGKHAKFRCYVTGKPKPEIL----WRKDGKLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCSASNTAGQTLSA 332
[Close]
BLAST of Titin vs. Ensembl Xenopus
Match: PALLD (palladin, cytoskeletal associated protein [Source:NCBI gene;Acc:100144658])

HSP 1 Score: 62.003 bits (149), Expect = 4.024e-9
Identity = 73/294 (24.83%), Postives = 120/294 (40.82%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVA------------------------------KSKSG---QNQSNPATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCA--------LDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSIS 1123
            A RF+  L ++  V E   ++L  +    G+    + W  +GK L          +  G   L I  + EED+G Y CVA                              KSK G   Q Q   ++V +T     P+  +   V    +  VQQ  +   Y  +  S      +P F+K+++ N+T ++G  V LE  V  +    +KV W + G  +     F  +  +  CA        L I  T+PED G++TC A+N+ G    S +Q+T +      +++N++   IS
Sbjct:  281 AARFIQKLRSQ-EVAEGSRVKLECRAT--GNPLPEIRWFCEGKELSNSPDIHISSDSNGLHTLVIAEAFEEDTGRYTCVASNCLGSDTSSAEIFVEGASSSDSESESFAFKSKQGAMPQAQKKTSSVSLTIGPSSPKCGITTAVMQPISIPVQQVRSPTSYLYQDRSKTPTFCAPAFTKEMQ-NLTASEGQVVVLECRV--KGTPPVKVKWFRQGSEIQDSPDFRILQKKPRCASEPEEICTLVIAETFPEDAGIFTCTARNDYGSVSCS-AQLTVLSANNTHTRNNVSSEEIS 567
[Close]
BLAST of Titin vs. Ensembl Xenopus
Match: PALLD (palladin, cytoskeletal associated protein [Source:NCBI gene;Acc:100144658])

HSP 1 Score: 59.6918 bits (143), Expect = 1.952e-8
Identity = 70/277 (25.27%), Postives = 112/277 (40.43%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVA------------------------------KSKSG---QNQSNPATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCA--------LDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            A RF+  L ++  V E   ++L  +    G+    + W  +GK L          +  G   L I  + EED+G Y CVA                              KSK G   Q Q   ++V +T     P+  +   V    +  VQQ  +   Y  +  S      +P F+K+++ N+T ++G  V LE  V  +    +KV W + G  +     F  +  +  CA        L I  T+PED G++TC A+N+ G    S +Q+T +
Sbjct:  246 AARFIQKLRSQ-EVAEGSRVKLECRAT--GNPLPEIRWFCEGKELSNSPDIHISSDSNGLHTLVIAEAFEEDTGRYTCVASNCLGSDTSSAEIFVEGASSSDSESESFAFKSKQGAMPQAQKKTSSVSLTIGPSSPKCGITTAVMQPISIPVQQVRSPTSYLYQDRSKTPTFCAPAFTKEMQ-NLTASEGQVVVLECRV--KGTPPVKVKWFRQGSEIQDSPDFRILQKKPRCASEPEEICTLVIAETFPEDAGIFTCTARNDYGSVSCS-AQLTVL 515
[Close]
BLAST of Titin vs. Ensembl Xenopus
Match: PALLD (palladin, cytoskeletal associated protein [Source:NCBI gene;Acc:100144658])

HSP 1 Score: 59.3066 bits (142), Expect = 2.052e-8
Identity = 70/277 (25.27%), Postives = 112/277 (40.43%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVA------------------------------KSKSG---QNQSNPATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCA--------LDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            A RF+  L ++  V E   ++L  +    G+    + W  +GK L          +  G   L I  + EED+G Y CVA                              KSK G   Q Q   ++V +T     P+  +   V    +  VQQ  +   Y  +  S      +P F+K+++ N+T ++G  V LE  V  +    +KV W + G  +     F  +  +  CA        L I  T+PED G++TC A+N+ G    S +Q+T +
Sbjct:  269 AARFIQKLRSQ-EVAEGSRVKLECRAT--GNPLPEIRWFCEGKELSNSPDIHISSDSNGLHTLVIAEAFEEDTGRYTCVASNCLGSDTSSAEIFVEGASSSDSESESFAFKSKQGAMPQAQKKTSSVSLTIGPSSPKCGITTAVMQPISIPVQQVRSPTSYLYQDRSKTPTFCAPAFTKEMQ-NLTASEGQVVVLECRV--KGTPPVKVKWFRQGSEIQDSPDFRILQKKPRCASEPEEICTLVIAETFPEDAGIFTCTARNDYGSVSCS-AQLTVL 538
[Close]
BLAST of Titin vs. Ensembl Xenopus
Match: PALLD (palladin, cytoskeletal associated protein [Source:NCBI gene;Acc:100144658])

HSP 1 Score: 59.3066 bits (142), Expect = 2.193e-8
Identity = 70/277 (25.27%), Postives = 112/277 (40.43%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVA------------------------------KSKSG---QNQSNPATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCA--------LDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            A RF+  L ++  V E   ++L  +    G+    + W  +GK L          +  G   L I  + EED+G Y CVA                              KSK G   Q Q   ++V +T     P+  +   V    +  VQQ  +   Y  +  S      +P F+K+++ N+T ++G  V LE  V  +    +KV W + G  +     F  +  +  CA        L I  T+PED G++TC A+N+ G    S +Q+T +
Sbjct:  246 AARFIQKLRSQ-EVAEGSRVKLECRAT--GNPLPEIRWFCEGKELSNSPDIHISSDSNGLHTLVIAEAFEEDTGRYTCVASNCLGSDTSSAEIFVEGASSSDSESESFAFKSKQGAMPQAQKKTSSVSLTIGPSSPKCGITTAVMQPISIPVQQVRSPTSYLYQDRSKTPTFCAPAFTKEMQ-NLTASEGQVVVLECRV--KGTPPVKVKWFRQGSEIQDSPDFRILQKKPRCASEPEEICTLVIAETFPEDAGIFTCTARNDYGSVSCS-AQLTVL 515
[Close]
BLAST of Titin vs. Ensembl Xenopus
Match: PALLD (palladin, cytoskeletal associated protein [Source:NCBI gene;Acc:100144658])

HSP 1 Score: 59.3066 bits (142), Expect = 2.200e-8
Identity = 70/277 (25.27%), Postives = 112/277 (40.43%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVA------------------------------KSKSG---QNQSNPATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCA--------LDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            A RF+  L ++  V E   ++L  +    G+    + W  +GK L          +  G   L I  + EED+G Y CVA                              KSK G   Q Q   ++V +T     P+  +   V    +  VQQ  +   Y  +  S      +P F+K+++ N+T ++G  V LE  V  +    +KV W + G  +     F  +  +  CA        L I  T+PED G++TC A+N+ G    S +Q+T +
Sbjct:  246 AARFIQKLRSQ-EVAEGSRVKLECRAT--GNPLPEIRWFCEGKELSNSPDIHISSDSNGLHTLVIAEAFEEDTGRYTCVASNCLGSDTSSAEIFVEGASSSDSESESFAFKSKQGAMPQAQKKTSSVSLTIGPSSPKCGITTAVMQPISIPVQQVRSPTSYLYQDRSKTPTFCAPAFTKEMQ-NLTASEGQVVVLECRV--KGTPPVKVKWFRQGSEIQDSPDFRILQKKPRCASEPEEICTLVIAETFPEDAGIFTCTARNDYGSVSCS-AQLTVL 515
[Close]
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864])

HSP 1 Score: 105.531 bits (262), Expect = 1.454e-22
Identity = 116/470 (24.68%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   + H+  T I  ++   KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1516 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYG---TDHTSATLIVKDE---KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 1960

HSP 2 Score: 62.7734 bits (151), Expect = 1.713e-9
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  900 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1128

HSP 3 Score: 58.9214 bits (141), Expect = 2.809e-8
Identity = 77/345 (22.32%), Postives = 131/345 (37.97%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIND-----------AFRNTEDQEDDQ--------------------APSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV 1330
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +     P+      S L+E    Q+   E  ++EE+ +           AF ++ED +                        P+F + L      EG    F  ++ ++P   + W  NN  +
Sbjct: 3197 EPPQVLQELQP-VTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATLRLEAPE------SILHERIGQQI---EMEMKEELFEGEADGSGDARGAFSDSEDIDHHSLMARRYANRTSSTSSWPEYFKPTFTQKLTFQYALEGEPIVFTCSLVAYPTPEMTWFHNNRPI 3503
[Close]
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864])

HSP 1 Score: 105.145 bits (261), Expect = 1.923e-22
Identity = 117/470 (24.89%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   +S + I  + DE    KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1516 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDE----KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 1960

HSP 2 Score: 77.0258 bits (188), Expect = 7.737e-14
Identity = 89/388 (22.94%), Postives = 155/388 (39.95%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPD----EKIVSKSNLNESSISQLKKFENNIQEEIND----------------AFRNTE--DQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +     P+    E+I  +  +    I+ L   E + Q   +D                  R+T+   +++  AP F + + DV I   + AK  + +   P+  I W  N   +   +  KF  DG  + L +  TR  + G++    +A+N  G+      L ++
Sbjct: 3197 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYT--CLASNEYGKAVCSAHLRIS 3553

HSP 3 Score: 63.1586 bits (152), Expect = 1.619e-9
Identity = 87/341 (25.51%), Postives = 143/341 (41.94%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEW-LKDGKPLLTGTRF--STVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRV-TPEAKVDNNVN-------------FIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGA-VHLETIVD--------PRNDVTL------KVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAE-SSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            P  ++ L++ +T +E  P R   QV+      L V W  KD K  +  +RF   T FE  Y  L+I  +  ED G Y  VA +  GQ  S  AT+R+  PE+ +   +                  +  Q   ++ + P+ +E+    S+ P  S   +S     +G A V +  I D         +  VT+      K+ W  NG  ++  + +  VFD    +L IL+T  +D+G YTC+A N  G+A  S+H +I+                      + E + + E  +  +  E  +    P F K L+ V    G  A F  ++H  P  +++W
Sbjct: 3305 PAIVTPLQDTVT-SEGRPARFQCQVS---GTDLKVSWYCKDKK--IKPSRFFRMTQFEDTYQ-LEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSR---QEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKAVCSAHLRIS---------------------PRGERSTEMESGEK-KALEKPKGPCPPYFFKELKPVHCGPGIPAVFEYSVHGEPAPTVLW 3612

HSP 4 Score: 62.3882 bits (150), Expect = 2.234e-9
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  900 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1128

HSP 5 Score: 53.9138 bits (128), Expect = 1.008e-6
Identity = 61/234 (26.07%), Postives = 94/234 (40.17%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   E++  P F K L +N   ++G +V  E  V      TLK  W K+GQ +SLG     V +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 25182 VTWYKSGQKIKPGDDEKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKHGED-SCKAKLTVT------------------------LHPPPTETTLRPMF-KRLLANAECHEGQSVCFEIRVSGIPAPTLK--WEKDGQPLSLGPHIEIVHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYVKQEFQSKEERERHVQKQIDKTLRMAE 25378
[Close]
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864])

HSP 1 Score: 105.145 bits (261), Expect = 2.048e-22
Identity = 117/470 (24.89%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   +S + I  + DE    KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1562 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDE----KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 2006

HSP 2 Score: 71.2478 bits (173), Expect = 5.134e-12
Identity = 80/364 (21.98%), Postives = 134/364 (36.81%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +                      +L+  E+ + E I        + E   AP   + ++ + +  G+ AKF   I   P     W     E+             ++ LE+ R   V        A+N  G ++    LTV 
Sbjct: 3243 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATL---------------------RLEAPESILHERIGQQI----EMEMKAAPVIKRRIEPLEVALGHLAKFTCEIQGAPNVRFQWFKAGREIYESDKCSIRSSNYVSSLEILRTQVVDCGEYTCKASNEYGSVSCTATLTVT 3552

HSP 3 Score: 64.6994 bits (156), Expect = 5.392e-10
Identity = 76/341 (22.29%), Postives = 132/341 (38.71%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSH--------------SQITCIPDEKIVSKSNLNESSISQLKKFENNIQ----------EEINDAFRN-------------TEDQEDDQAPSFDKGLQ-DVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            + V E+DP+ L   VA  G   L V+WLKDGK ++    FS  FE       I   +++DSG Y    ++  G +  + A +RV                    D++ P           P F+K L + +    G ++H+E  V     + +   W K+G+ +S  +++  V      +L++     ED   YTC   N  G+   S                +   IPD  +  K+ L  +   ++K  +++++           E + +F N              +   D + P F K L+    I  G++A+    I   P   +VW  N  E+ +
Sbjct: 5156 VDVTEKDPVTLECVVA--GTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRVL-------------------DQDIP-----------PSFTKKL-TKMDKVLGSSIHMECKVS--GSLPISAQWFKDGKEISTSAKYRLVCHENTVSLEVSNLELEDTANYTCKVSNVAGDNACSGILTVKEPPSFLVKPERQQAIPDSTVEFKAVLKGTPPFKIKWLKDDVELVSGPKCFIGLEGSTSFLNLYSVDSSKTGQYTCQVTNDVEPPKFVKKLEASKIIKAGDSARLECKITGSPEIQVVWYRNEHELTA 5460

HSP 4 Score: 62.7734 bits (151), Expect = 1.971e-9
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  946 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1174

HSP 5 Score: 55.0694 bits (131), Expect = 4.152e-7
Identity = 84/411 (20.44%), Postives = 156/411 (37.96%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS--------------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            A R L+     ITV+E +  R S      G+   +V WL++G+ + T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +       + V+  E    +P     +++              P  ++ L++  + +      L   V+       +V W+K+G+ +     F   +   G   L I      D G Y C      G +++S           H Q++ I +    +KS    +  ++ KK E    E I+              P    GL+D T+   + AKF I +   P+ +I W+ + + +   S  K   +     LE+  T  ++ G +   V  TN  G ++S C LT+ ++ +
Sbjct: 32603 AARILTK-PRSITVHEGESARFSCDTD--GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFTLTVQKARVIEKAVTSPPRVK-SPEPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKD---IAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTSFQFTGQSFKSIHEQVSSISE---TTKSVQKTAESAEAKKQEPIAPESIS------------SKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTV--TNSAGSVSSSCKLTIKAVKD 32989

HSP 6 Score: 53.9138 bits (128), Expect = 9.120e-7
Identity = 61/234 (26.07%), Postives = 94/234 (40.17%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   E++  P F K L +N   ++G +V  E  V      TLK  W K+GQ +SLG     V +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 31763 VTWYKSGQKIKPGDDEKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKHGED-SCKAKLTVT------------------------LHPPPTETTLRPMF-KRLLANAECHEGQSVCFEIRVSGIPAPTLK--WEKDGQPLSLGPHIEIVHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYVKQEFQSKEERERHVQKQIDKTLRMAE 31959

HSP 7 Score: 53.9138 bits (128), Expect = 9.676e-7
Identity = 82/329 (24.92%), Postives = 143/329 (43.47%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA--KVDNNVNFIE--NQHVQQD----ENEPQYPDKHES-SQSPYFSKDL-ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHS-QITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFII--NIHSHPRASIVWSINNEEVISDS 1342
            P+   +L+ E +   +D  +L+  V  +   +  V W KDGK L     F   +   G   L I    E D G YVC     SG+  ++  + + T ++   +   V+ I    + VQ+     E + Q P   ES S  P     L ++ ++ +      ++   +P+  +T    W K+G++++ GS++    ++    L+IL T   D G+Y C   N+ G   SS    I  + D +    S    S ++  KK   + QEEI+     +E+ +  +     K  + + I E  A+K +I   +     AS+  SI +EEV   S
Sbjct: 32761 PKITQSLKAEAS---KDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEV---SGEGGTSKTSFQFTGQSFKSIHEQVSSISETTKSVQKTAESAEAKKQEPIAPESISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTIT----WTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKK--ASAQEEISQKALTSEEIKMSEV----KSHETLAIKE-EASKVLIAEEVKRSAAASLEKSIVHEEVTKTS 33072

HSP 8 Score: 52.373 bits (124), Expect = 2.693e-6
Identity = 79/372 (21.24%), Postives = 125/372 (33.60%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGD-GTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +AP       N+ ++ +  P+ L    +  G   +SV W K+G  ++   R +        +L+IL S  EDSG Y C  ++ SG++  +   + + P                                  PYF K LE   +TV    ++  +    P     + V W+K    +   +     F      L        D G Y C A+N+ GE  SS                                        F     QE    PSF + L+DV    G    F   +      S+ W  + +  + DS N    F  +  T+N  +  R    GQ++    ATNP+G  +S   L +    N
Sbjct: 7859 KAPAIFVKRLNDYSIEKGKPLILEGTFS--GTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEP----------------------------------PYFVKQLEPVKVTVGDSASLQCQLAGTPE----IGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS---------------------------------------TFLTV--QEQKLPPSFSRQLRDVQETVGLPVVFECAVSGSEPISVSWYKDGKP-LKDSPNIQTSFLDNIATLNIFKTDRSLS-GQYS--CTATNPIGSASSGAKLILTEGKN 8145

HSP 9 Score: 52.373 bits (124), Expect = 3.057e-6
Identity = 62/232 (26.72%), Postives = 97/232 (41.81%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVR-------VTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKN-GQSMSLGSRFNAVFDRGYCA-LDILYTYPEDDGVYTCVAKNNCGEAES 1048
            KE + P         +T +E D ++LS  V   G   + ++WLK G+ +    R S  F  G  +L++  + + DSG YVC A + +G + S    T++          +A VD  + F+         +EPQ     E + + + +K          GG            D    V W K   + ++ G R   +  +G  A L+I  T   D G+Y CVA N  GE ES
Sbjct: 8334 KEPKKPPVFDQHLAPVTASEGDSVQLSCHVQ--GSEPIRIQWLKAGREVKPSDRCSFSFASGTAMLELKETAKADSGDYVCKASNVAGSDTSKCKVTIKEKPAAAPAAKKAAVDGKLFFV---------SEPQSIRVVEKTTATFIAK---------VGG------------DPIPNVKWTKGKWRQLNQGGRI-LIHQKGDEAKLEIRDTTKTDSGLYRCVAFNKHGEIES 8532

HSP 10 Score: 51.9878 bits (123), Expect = 4.324e-6
Identity = 50/215 (23.26%), Postives = 85/215 (39.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            E+P F    ++ I V +E  + L ++VA  G     + W KD   L +G ++ T  +   V L +L  +  D+G Y C   ++ G   S+  + RVT                      EP           P F K +E+  ++  G A    T+   +  + + V W K+   ++        F+    +L +     + DG Y C AKN+ G
Sbjct: 4397 ESPYFTKEFKS-IEVLKEYDVMLLAEVA--GTPPFEITWFKDNTTLRSGRKYKTFLQDQLVSLQVLKFVAADAGEYQCRVTNEVG---SSTCSARVT--------------------LREP-----------PSFIKKIEATSSLRGGTAAFQATL---KGSLPITVTWLKDNDEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAG 4571
[Close]
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864])

HSP 1 Score: 105.145 bits (261), Expect = 2.048e-22
Identity = 117/470 (24.89%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   +S + I  + DE    KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1562 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDE----KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 2006

HSP 2 Score: 71.2478 bits (173), Expect = 5.134e-12
Identity = 80/364 (21.98%), Postives = 134/364 (36.81%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +                      +L+  E+ + E I        + E   AP   + ++ + +  G+ AKF   I   P     W     E+             ++ LE+ R   V        A+N  G ++    LTV 
Sbjct: 3243 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATL---------------------RLEAPESILHERIGQQI----EMEMKAAPVIKRRIEPLEVALGHLAKFTCEIQGAPNVRFQWFKAGREIYESDKCSIRSSNYVSSLEILRTQVVDCGEYTCKASNEYGSVSCTATLTVT 3552

HSP 3 Score: 64.6994 bits (156), Expect = 5.392e-10
Identity = 76/341 (22.29%), Postives = 132/341 (38.71%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSH--------------SQITCIPDEKIVSKSNLNESSISQLKKFENNIQ----------EEINDAFRN-------------TEDQEDDQAPSFDKGLQ-DVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            + V E+DP+ L   VA  G   L V+WLKDGK ++    FS  FE       I   +++DSG Y    ++  G +  + A +RV                    D++ P           P F+K L + +    G ++H+E  V     + +   W K+G+ +S  +++  V      +L++     ED   YTC   N  G+   S                +   IPD  +  K+ L  +   ++K  +++++           E + +F N              +   D + P F K L+    I  G++A+    I   P   +VW  N  E+ +
Sbjct: 5156 VDVTEKDPVTLECVVA--GTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRVL-------------------DQDIP-----------PSFTKKL-TKMDKVLGSSIHMECKVS--GSLPISAQWFKDGKEISTSAKYRLVCHENTVSLEVSNLELEDTANYTCKVSNVAGDNACSGILTVKEPPSFLVKPERQQAIPDSTVEFKAVLKGTPPFKIKWLKDDVELVSGPKCFIGLEGSTSFLNLYSVDSSKTGQYTCQVTNDVEPPKFVKKLEASKIIKAGDSARLECKITGSPEIQVVWYRNEHELTA 5460

HSP 4 Score: 62.7734 bits (151), Expect = 1.971e-9
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  946 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1174

HSP 5 Score: 55.4546 bits (132), Expect = 3.221e-7
Identity = 84/411 (20.44%), Postives = 156/411 (37.96%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS--------------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            A R L+     ITV+E +  R S      G+   +V WL++G+ + T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +       + V+  E    +P     +++              P  ++ L++  + +      L   V+       +V W+K+G+ +     F   +   G   L I      D G Y C      G +++S           H Q++ I +    +KS    +  ++ KK E    E I+              P    GL+D T+   + AKF I +   P+ +I W+ + + +   S  K   +     LE+  T  ++ G +   V  TN  G ++S C LT+ ++ +
Sbjct: 32603 AARILTK-PRSITVHEGESARFSCDTD--GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFTLTVQKARVIEKAVTSPPRVK-SPEPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKD---IAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTSFQFTGQSFKSIHEQVSSISE---TTKSVQKTAESAEAKKSEPIAPESIS------------SKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTV--TNSAGSVSSSCKLTIKAVKD 32989

HSP 6 Score: 53.9138 bits (128), Expect = 9.120e-7
Identity = 61/234 (26.07%), Postives = 94/234 (40.17%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   E++  P F K L +N   ++G +V  E  V      TLK  W K+GQ +SLG     V +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 31763 VTWYKSGQKIKPGDDEKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKHGED-SCKAKLTVT------------------------LHPPPTETTLRPMF-KRLLANAECHEGQSVCFEIRVSGIPAPTLK--WEKDGQPLSLGPHIEIVHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYVKQEFQSKEERERHVQKQIDKTLRMAE 31959

HSP 7 Score: 52.373 bits (124), Expect = 2.693e-6
Identity = 79/372 (21.24%), Postives = 125/372 (33.60%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGD-GTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +AP       N+ ++ +  P+ L    +  G   +SV W K+G  ++   R +        +L+IL S  EDSG Y C  ++ SG++  +   + + P                                  PYF K LE   +TV    ++  +    P     + V W+K    +   +     F      L        D G Y C A+N+ GE  SS                                        F     QE    PSF + L+DV    G    F   +      S+ W  + +  + DS N    F  +  T+N  +  R    GQ++    ATNP+G  +S   L +    N
Sbjct: 7859 KAPAIFVKRLNDYSIEKGKPLILEGTFS--GTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEP----------------------------------PYFVKQLEPVKVTVGDSASLQCQLAGTPE----IGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS---------------------------------------TFLTV--QEQKLPPSFSRQLRDVQETVGLPVVFECAVSGSEPISVSWYKDGKP-LKDSPNIQTSFLDNIATLNIFKTDRSLS-GQYS--CTATNPIGSASSGAKLILTEGKN 8145

HSP 8 Score: 52.373 bits (124), Expect = 2.739e-6
Identity = 78/331 (23.56%), Postives = 141/331 (42.60%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA--KVDNNVNFIEN---------QHVQQDENEPQYPDKHESSQSPYFSKDL-ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHS-QITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFII--NIHSHPRASIVWSINNEEVISDS 1342
            P+   +L+ E +   +D  +L+  V  +   +  V W KDGK L     F   +   G   L I    E D G YVC     SG+  ++  + + T ++   +   V+ I           +  +  ++EP  P+    S  P     L ++ ++ +      ++   +P+  +T    W K+G++++ GS++    ++    L+IL T   D G+Y C   N+ G   SS    I  + D +    S    S ++  KK   + QEEI+     +E+ +  +     K  + + I E  A+K +I   +     AS+  SI +EEV   S
Sbjct: 32761 PKITQSLKAEAS---KDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEV---SGEGGTSKTSFQFTGQSFKSIHEQVSSISETTKSVQKTAESAEAKKSEPIAPE--SISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTIT----WTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKK--ASAQEEISQKALTSEEIKMSEV----KSHETLAIKE-EASKVLIAEEVKRSAAASLEKSIVHEEVTKTS 33072

HSP 9 Score: 52.373 bits (124), Expect = 3.057e-6
Identity = 62/232 (26.72%), Postives = 97/232 (41.81%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVR-------VTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKN-GQSMSLGSRFNAVFDRGYCA-LDILYTYPEDDGVYTCVAKNNCGEAES 1048
            KE + P         +T +E D ++LS  V   G   + ++WLK G+ +    R S  F  G  +L++  + + DSG YVC A + +G + S    T++          +A VD  + F+         +EPQ     E + + + +K          GG            D    V W K   + ++ G R   +  +G  A L+I  T   D G+Y CVA N  GE ES
Sbjct: 8334 KEPKKPPVFDQHLAPVTASEGDSVQLSCHVQ--GSEPIRIQWLKAGREVKPSDRCSFSFASGTAMLELKETAKADSGDYVCKASNVAGSDTSKCKVTIKEKPAAAPAAKKAAVDGKLFFV---------SEPQSIRVVEKTTATFIAK---------VGG------------DPIPNVKWTKGKWRQLNQGGRI-LIHQKGDEAKLEIRDTTKTDSGLYRCVAFNKHGEIES 8532

HSP 10 Score: 51.9878 bits (123), Expect = 4.324e-6
Identity = 50/215 (23.26%), Postives = 85/215 (39.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            E+P F    ++ I V +E  + L ++VA  G     + W KD   L +G ++ T  +   V L +L  +  D+G Y C   ++ G   S+  + RVT                      EP           P F K +E+  ++  G A    T+   +  + + V W K+   ++        F+    +L +     + DG Y C AKN+ G
Sbjct: 4397 ESPYFTKEFKS-IEVLKEYDVMLLAEVA--GTPPFEITWFKDNTTLRSGRKYKTFLQDQLVSLQVLKFVAADAGEYQCRVTNEVG---SSTCSARVT--------------------LREP-----------PSFIKKIEATSSLRGGTAAFQATL---KGSLPITVTWLKDNDEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAG 4571
[Close]
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864])

HSP 1 Score: 104.76 bits (260), Expect = 2.475e-22
Identity = 117/470 (24.89%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   +S + I  + DE    KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1562 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDE----KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 2006

HSP 2 Score: 72.0182 bits (175), Expect = 2.650e-12
Identity = 104/397 (26.20%), Postives = 165/397 (41.56%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEW-LKDGKPLLTGTRF--STVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES-SQSPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVF----DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC------IPDEKIVSKSNLNESSISQLKKFENNIQEEIND----------------AFRNTE--DQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P  ++ L++ +T +E  P R   QV+      L V W  KD K  +  +RF   T FE  Y  L+I  +  ED G Y  VA +  GQ  S  AT+R+   +K +      E     Q    P    K E+  Q P F + L S++ V+ G  V     V   P+      + W  N Q + L S+ + VF      G   + I+  YPE  G Y+C A N+ GEA  + +          I  E+I  +  +    I+ L   E + Q   +D                  R+T+   +++  AP F + + DV I   + AK  + +   P+  I W  N   +   +  KF  DG  + L +  TR  + G++    +A+N  G+      L ++
Sbjct: 3351 PAIVTPLQDTVT-SEGRPARFQCQVS---GTDLKVSWYCKDKK--IKPSRFFRMTQFEDTYQ-LEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEGFSKSE------EVTSKSQWHTSPSVSLKKEALGQKPIFIQPL-SSLRVHSGETVRFHARVSGVPKP----AIQWFHNHQPI-LPSK-DVVFHFEESTGMALMLIVDAYPEHAGKYSCRAANSAGEATCAATLTVTAKAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYT--CLASNEYGKAVCSAHLRIS 3724

HSP 3 Score: 68.5514 bits (166), Expect = 3.180e-11
Identity = 86/371 (23.18%), Postives = 144/371 (38.81%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT--CIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINYLEL---TRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +         E++ SKS  + S    LKK                  +   Q P F + L  + +  G   +F   +   P+ +I W  N++ ++ S  V   + + T   L L         G+++ R  A N  G+      LTV +
Sbjct: 3243 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATLRLEGFSKSEEVTSKSQWHTSPSVSLKK------------------EALGQKPIFIQPLSSLRVHSGETVRFHARVSGVPKPAIQWFHNHQPILPSKDVVFHFEESTGMALMLIVDAYPEHAGKYSCR--AANSAGEATCAATLTVTA 3564

HSP 4 Score: 62.3882 bits (150), Expect = 2.355e-9
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  946 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1174

HSP 5 Score: 60.077 bits (144), Expect = 1.138e-8
Identity = 78/353 (22.10%), Postives = 133/353 (37.68%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSH--------------SQITCIPDEKIVSKSNLNESSISQLKKFENNIQ----------EEINDAFRN----------------TEDQEDD---------QAPSFDKGLQ-DVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            + V E+DP+ L   VA  G   L V+WLKDGK ++    FS  FE       I   +++DSG Y    ++  G +  + A +RV                    D++ P           P F+K L + +    G ++H+E  V     + +   W K+G+ +S  +++  V      +L++     ED   YTC   N  G+   S                +   IPD  +  K+ L  +   ++K  +++++           E + +F N                T D   D         + P F K L+    I  G++A+    I   P   +VW  N  E+ +
Sbjct: 6163 VDVTEKDPVTLECVVA--GTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRVL-------------------DQDIP-----------PSFTKKL-TKMDKVLGSSIHMECKVS--GSLPISAQWFKDGKEISTSAKYRLVCHENTVSLEVSNLELEDTANYTCKVSNVAGDNACSGILTVKEPPSFLVKPERQQAIPDSTVEFKAVLKGTPPFKIKWLKDDVELVSGPKCFIGLEGSTSFLNLYSVDSSKTGQYTCQVTNDVGSDSCTTMLLVTEPPKFVKKLEASKIIKAGDSARLECKITGSPEIQVVWYRNEHELTA 6479

HSP 6 Score: 58.5362 bits (140), Expect = 3.421e-8
Identity = 77/344 (22.38%), Postives = 145/344 (42.15%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER--GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT---PEAKVDNNVN-------------FIENQHVQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGA-VHLETIVD--------PRNDVTL------KVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAE-SSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            + P F+  L + + V+  + +R  ++V+  G    +++W  + +P+L        FE   G  ++ I+ +  E +G Y C A + +G+  +  AT+ VT   PE+ +   +                  +  Q   ++ + P+ +E+    S+ P  S   +S     +G A V +  I D         +  VT+      K+ W  NG  ++  + +  VFD    +L IL+T  +D+G YTC+A N  G+A  S+H +I+                      + E + + E  +  +  E  +    P F K L+ V    G  A F  ++H  P  +++W
Sbjct: 3469 QKPIFIQPLSS-LRVHSGETVRFHARVS--GVPKPAIQWFHNHQPILPSKDVVFHFEESTGMALMLIVDAYPEHAGKYSCRAANSAGE-ATCAATLTVTAKAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSR---QEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKAVCSAHLRIS---------------------PRGERSTEMESGEK-KALEKPKGPCPPYFFKELKPVHCGPGIPAVFEYSVHGEPAPTVLW 3783

HSP 7 Score: 53.9138 bits (128), Expect = 8.819e-7
Identity = 61/234 (26.07%), Postives = 94/234 (40.17%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   E++  P F K L +N   ++G +V  E  V      TLK  W K+GQ +SLG     V +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 33634 VTWYKSGQKIKPGDDEKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKHGED-SCKAKLTVT------------------------LHPPPTETTLRPMF-KRLLANAECHEGQSVCFEIRVSGIPAPTLK--WEKDGQPLSLGPHIEIVHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYVKQEFQSKEERERHVQKQIDKTLRMAE 33830

HSP 8 Score: 52.373 bits (124), Expect = 2.787e-6
Identity = 79/372 (21.24%), Postives = 125/372 (33.60%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGD-GTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +AP       N+ ++ +  P+ L    +  G   +SV W K+G  ++   R +        +L+IL S  EDSG Y C  ++ SG++  +   + + P                                  PYF K LE   +TV    ++  +    P     + V W+K    +   +     F      L        D G Y C A+N+ GE  SS                                        F     QE    PSF + L+DV    G    F   +      S+ W  + +  + DS N    F  +  T+N  +  R    GQ++    ATNP+G  +S   L +    N
Sbjct: 8878 KAPAIFVKRLNDYSIEKGKPLILEGTFS--GTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEP----------------------------------PYFVKQLEPVKVTVGDSASLQCQLAGTPE----IGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS---------------------------------------TFLTV--QEQKLPPSFSRQLRDVQETVGLPVVFECAVSGSEPISVSWYKDGKP-LKDSPNIQTSFLDNIATLNIFKTDRSLS-GQYS--CTATNPIGSASSGAKLILTEGKN 9164

HSP 9 Score: 52.373 bits (124), Expect = 3.190e-6
Identity = 62/232 (26.72%), Postives = 97/232 (41.81%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVR-------VTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKN-GQSMSLGSRFNAVFDRGYCA-LDILYTYPEDDGVYTCVAKNNCGEAES 1048
            KE + P         +T +E D ++LS  V   G   + ++WLK G+ +    R S  F  G  +L++  + + DSG YVC A + +G + S    T++          +A VD  + F+         +EPQ     E + + + +K          GG            D    V W K   + ++ G R   +  +G  A L+I  T   D G+Y CVA N  GE ES
Sbjct: 9353 KEPKKPPVFDQHLAPVTASEGDSVQLSCHVQ--GSEPIRIQWLKAGREVKPSDRCSFSFASGTAMLELKETAKADSGDYVCKASNVAGSDTSKCKVTIKEKPAAAPAAKKAAVDGKLFFV---------SEPQSIRVVEKTTATFIAK---------VGG------------DPIPNVKWTKGKWRQLNQGGRI-LIHQKGDEAKLEIRDTTKTDSGLYRCVAFNKHGEIES 9551

HSP 10 Score: 51.6026 bits (122), Expect = 4.590e-6
Identity = 50/215 (23.26%), Postives = 85/215 (39.53%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            E+P F    ++ I V +E  + L ++VA  G     + W KD   L +G ++ T  +   V L +L  +  D+G Y C   ++ G   S+  + RVT                      EP           P F K +E+  ++  G A    T+   +  + + V W K+   ++        F+    +L +     + DG Y C AKN+ G
Sbjct: 5404 ESPYFTKEFKS-IEVLKEYDVMLLAEVA--GTPPFEITWFKDNTTLRSGRKYKTFLQDQLVSLQVLKFVAADAGEYQCRVTNEVG---SSTCSARVT--------------------LREP-----------PSFIKKIEATSSLRGGTAAFQATL---KGSLPITVTWLKDNDEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAG 5578
[Close]
BLAST of Titin vs. UniProt/SwissProt
Match: sp|Q9I7U4|TITIN_DROME (Titin OS=Drosophila melanogaster OX=7227 GN=sls PE=1 SV=3)

HSP 1 Score: 175.252 bits (443), Expect = 8.429e-44
Identity = 118/400 (29.50%), Postives = 197/400 (49.25%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATV--RVTPEAKVDNNV------------------NFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQ---EEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQ-MASDCILTVNSINNYAQDLK 1510
            F  P+F + +   +TV+E + +R   QV P  D SL VEW ++GKPL +G R+  +F+ G+V LDILY   EDSG YVC A +  G++++  ATV  +  P   + N V                   +    H+ +D+     PD+    Q P F   ++  +T+ +      E  + P  D  +KV+W  NG+ +   +RF  ++D GY A++  + YPED G Y C A N  G+ E+  + I       IV  S L  +  SI ++++ E + Q   +E++       D +    P F   L+  T++EG+ A+F + +  HPR  ++W IN   V+  S  K   DG   +L++ +  +     + V+A N +G+ +A+  +  V   ++Y   LK
Sbjct: 3808 FGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDL--FDPDR---KQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDET-RAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDP------DAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLK 4192

HSP 2 Score: 158.688 bits (400), Expect = 2.176e-38
Identity = 112/380 (29.47%), Postives = 183/380 (48.16%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVD-NNVNFIENQHVQQDEN----EPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD----QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINY--LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            +APRFL  L+    + E       ++V P  D  L +EW  +G+ +    R  T ++ GYV LDI     ED+GVY+ VA++K G+ Q   AT+ V   + +D ++++    +  Q  EN    EPQY  +  S   P F   L     ++ G  +HLE  ++P  D T++V+W  NG+ +++GSRF   +D G+ ALDI+     D G YT  A N+ G A +S   +  I    +V+++  NE S+ Q++  E++         R    QE+D    QAP F +GL ++   EG        +     P   I W +N + V +   ++F      +Y  L+L  C  +        A N LG+  + C + + + N+
Sbjct: 1022 QAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQ-ATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSAC-VRVIDHTDVVTETQ-NEQSLEQIQLLEDS---------RRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTG--HRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKND 1387

HSP 3 Score: 157.918 bits (398), Expect = 3.274e-38
Identity = 119/397 (29.97%), Postives = 195/397 (49.12%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD---KHESSQ--------SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNI----QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
             +AP+F   L N I   E   + L  ++ P GD S+ +EW  +GKP+ TG RF   +E  YV LD+L     DSGVY C A+++ G+  ++  +VR+     +  N   +E Q+ +    + QY +   +H  S+         P F    +S     +GG  H E  ++P  D  LKV+W KNG+ +++G RF  + D GY ALDI++   ED GVYTC A N  G ++ +  ++ C   E+IV+ +  NE+ + Q+   E+      +EEI+++ +        QAP F   L++V I E   A F   +I + S P   + W  NN  + S S  +F       ++ L    T   + G +  R  A N +G+  +  +  V++  +   + +H
Sbjct: 1288 MQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTS-CSVRI-----IAKNDLILETQN-ESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIG-SDETQVELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTK--------QAPVFTTSLKNVEIKENQRAHFECRLIPV-SDPSMRVEWYHNNLPLKSGS--RFTETNNFGFVALDIMSTLPEDAGTYTCR--AYNAVGEAITSAVAVVHTKKSIYLESQH 1661

HSP 4 Score: 143.665 bits (361), Expect = 1.236e-33
Identity = 117/406 (28.82%), Postives = 183/406 (45.07%), Query Frame = 2 
Query:  368 SQYESKEFE------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHESSQ----SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC---IPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVV--ATNPLGQMASDCILTVNSINNYAQDLKH 1513
            S+Y+ +E E      AP F++ L     + E        ++ P  D +L VEW  +GKPL TG RF T ++ G+  LDIL    EDSG Y C   +  G+   N   + VT  + + +     E QH      +Q  E+  ++  K +  Q     P F + L  N  VN+G  VHLE  + P ND T+KV+W+ NG+ +  G RF   +D G+ ALDILY + ED G Y C AKN  GEA +     TC   +   K +    L+   + ++++ E                +E  Q P F   L ++  + EG  A     +   + P   I W  N +++ +   +++       Y+ L      G+     +  ATN LG+  + C + V +  +   D +H
Sbjct: 1808 SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEA-INSIVLNVTSRSSIIH-----ETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL-RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVT-----TCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVV------EEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTG--HRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQH 2193

HSP 5 Score: 143.665 bits (361), Expect = 1.247e-33
Identity = 114/390 (29.23%), Postives = 189/390 (48.46%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS---------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKF---IINIHSHPRASIVWSINNEEVISDS--VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            + P FL+ L N   + E +   L  +V P  D +L +EW  +GK L TG R+ T  + GYV LDILY   ED+G Y+C A ++ G+   N   VRV     ++     ++ QH    E   +   K  ++++         P+F+ +L  +  + +G   H E  V P +D  L+++++ NG+ +   SRF+  FD GY +LDI +   ED G Y+  A N  G+A SS + +  IP   I+S +   E  + +++K E+    +  +     E     Q P F + LQ++  I+E   A F   +I +   P   + W   NE++I DS  + K +  G ++  +   R  + G +  R  A NPLG+  +   + V S  +   D +H
Sbjct: 2087 QKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEA-VNTCNVRV-----LNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLESTAPHQRQEP----ETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPV-GDPNLKVEW-YRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCR--AVNPLGEAVTTASMRVVSEASIQMDTQH 2460

HSP 6 Score: 141.739 bits (356), Expect = 4.990e-33
Identity = 104/365 (28.49%), Postives = 173/365 (47.40%), Query Frame = 2 
Query:  278 DSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV--------NFIENQHVQQDENEPQYPDKH-----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSIN 1318
            +S LP G  +    + +A     +G GS  +++  +    + P F+++   ++ + E        ++ P  D S+ V+W  +GK L  G+R  T+ + G+V+L+I    + DSG+Y C A +K G+     ATV    + K    +        NF       Q   E  +  +      E    P F+++++ N+ V +GG +H +  V+P  D T++++W  NG  M+ GSR + + D G+ ALD+ Y Y  D G YTC A N  G A +S +++TC     IV +S L E   S+ LK+ E      I +A +  E  E    P F   +  VT+DE  A +F   +   + P   + W  N
Sbjct: 3502 ESQLPKG--MEGTIDRIAEL---EGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGE-----ATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERG---RIPEAPKVVE--EVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRN 3849

HSP 7 Score: 138.658 bits (348), Expect = 4.016e-32
Identity = 103/390 (26.41%), Postives = 181/390 (46.41%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVT-------------PEAKVDNNVNFIENQHVQQDENEPQYPDK-HESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGE----AESSHSQITCIPDEKIVSKSNLNESSISQL----KKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL----TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P+F   +++ + V E  PI    +V P GD ++ +EW  +G  + TG+R   + + G++ LD+ Y    DSG Y C A +K G   +   + +VT             PE      +  +E   +      P+ P    E    P F+  + +++TV++  AV  E  V+P+ D +L+V+W++NG+ +  G R+  +FD G+ +LDILY Y ED G Y C A NN GE    A  S  ++  I  +  V +      +++Q+    KK+ + +    +D F    D +  Q P F   +++ +T+ E    KF   +     P   + W  N + ++    N+F       Y+ +        + G++  R  ATN  G+  +  I+ V+
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG---TATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRI------PEAPKVVEEVFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLF----DPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHK--NRFQPIYDFGYVAMNFGWVYPEDSGEYVCR--ATNLYGKDETRAIIKVS 4047

HSP 8 Score: 138.658 bits (348), Expect = 4.620e-32
Identity = 110/387 (28.42%), Postives = 176/387 (45.48%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH------VQQDENEPQYPDKHES----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQ-LKKFENNIQEEINDAFRNTEDQED-----DQAPSFDKGLQDVT-IDEGNAAKFIINIH--------SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQM-ASDCILTVN 1480
            P+F + ++N++ + E       +++ P GD+++ VEWLKDG+PL   +R +T    GYV L I      D+G Y C A +  GQ+ +      V     +  N    E+QH      +Q  E+  +Y  + E     +Q+P F   L+    + +G   H E  V+P++D+ L ++W+ NG+S++  +R    +D GY ALDI     ED GVY  VA+N  GEA+   + I       + ++S+++ SS+ + L +   N++   N  F   E Q D        P F   L D   I +G       NIH          P   + W  N   V   S  + Y D     L++ + T        V ATN LG    S C+  ++
Sbjct:  890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTT------VAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE---NKPF--VEPQYDIEEISKSKPVFVTPLSDPKPIHDGK------NIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVID 1252

HSP 9 Score: 129.413 bits (324), Expect = 3.260e-29
Identity = 82/254 (32.28%), Postives = 137/254 (53.94%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN---NVNFIENQHVQQDENEPQYPDKHES-----SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES-------SHSQITC---IPDEK 1084
            E + +  P+F+  L+N +   E   + + +++ P GD ++ VEW  +G+PL    R ++VF+ G++ LD+L  +  DSG Y+C   + SG  +S  A + V     ++    N N +  Q++ Q E+  +Y  + ES     +Q+P F + L       +G  VH E  V P ND +++V+W+K+G  ++  SR  A+F+ GY +L+IL+   ED G YT  A N  GEA S       S SQ+T    IP+++
Sbjct:  611 EDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL--QYINQLEDYSRY-QRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQ 859

HSP 10 Score: 129.413 bits (324), Expect = 3.946e-29
Identity = 99/341 (29.03%), Postives = 159/341 (46.63%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIEN-QHVQQDENE----PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELT 1390
            FE P F   L     + E       ++V P GD SL  EW  +G  L  G+R  T  + G+V LDI   + ED+GVY+C A + +G+  S+ A ++V  ++ +D      E+ + ++  E      P+        Q+P F+  L+S   +++G  V LE  V+PR D  L+++W KNG S++ GSR  + FD G   L I     +D  +YTC A N  GEA S+ S    I D   +   +L+  S+ ++ + E   +       R    +   + P F   L ++   E +  +F  N+     P  SI W  N + + + +  K   D     L+LT
Sbjct: 2485 FEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSS--LKIEDRHWLQAESLHPDSLPRIGELEAPKE------GRPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLT 2816

HSP 11 Score: 129.028 bits (323), Expect = 5.349e-29
Identity = 116/427 (27.17%), Postives = 187/427 (43.79%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL---NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVT----PELTTALHKPKP 1606
            E P+F++ + +   + E       +++ P  D  L VEW  +GK L  G RF T  + G V+LDILY  EE+SGVY   A++K G++ +  A+++   ++ +          +  +  I N          +   +    ++P F+  LE+   + +G   H E  + P +D  LKV+W+ NG+ +  GSRF    D G+  L+I   YPED G Y+C A N  GEA ++ + +       I+ +S L    E +I ++ + E           R+TE   DD   + P F     D+ I E   A F   +   + P   + W  N + + + S  K   D     LE+  C +         ATN  G+    C L V        + +   P N      SL+K++ T     EL T   +P P
Sbjct: 3261 EPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREET-TEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTAT-MKIQGKRSIIMESQLPKGMEGTIDRIAELEGL-------GSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQ--LPSNFRTGTESLQKLEETMHKREELVTEDEQPNP 3675

HSP 12 Score: 126.331 bits (316), Expect = 3.871e-28
Identity = 103/391 (26.34%), Postives = 190/391 (48.59%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA---KVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL-----NESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF-DKGLQDVTIDEGNAAKFIINIHSH--PRASIVWSINNEEVISDSVNKF-----YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKH 1513
            +AP F ++L+N + + E        ++ P  D S+ VEW  +  PL +G+RF+     G+V LDI+ ++ ED+G Y C A +  G+  ++   V  T ++   +  +       QH++      +   + E  SQ+P F+  +  ++ V +  AVH E  + P  D  L V+W +NGQ +   +R   + D GY AL++ Y  PED G YTC A N  G+A +S S I       + SK+++     +E+++ ++ + E++ + +     R  E+     AP F  K +    + EG +A +   I  +  P   + W  N + +   + ++F     +G   ++ L +    + G++  RV  TN LG+  +  +L V S ++   + +H
Sbjct: 1556 QAPVFTTSLKN-VEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPV-RDVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLI-------VQSKTSIQLETQHEAAMHKIHQLEDHSRYQ----RREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPL--STGHRFRTTYDFGFAALDILTV-YAEDSGEYTCRV--TNNLGEAINSIVLNVTSRSSIIHETQH 1928

HSP 13 Score: 123.635 bits (309), Expect = 2.494e-27
Identity = 72/232 (31.03%), Postives = 123/232 (53.02%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQD------------ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            +AP F ++L++   ++E   + L +QV P  D +L +EW K+G  L TG+R  + F+ G V L I     +DS +Y C A ++ G+  S       T   K+++  ++++ + +  D            E  P+ P+   + ++P F   L +NI   +   V  E  V+P  D T+ ++W  NGQ +   ++F +++D GYCALD+  +Y E+ GVYTC A N+ G A +S
Sbjct: 2620 QAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVS-------TSSLKIEDR-HWLQAESLHPDSLPRIGELEAPKEGRPEAPEP--TYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTS 2840

HSP 14 Score: 121.324 bits (303), Expect = 1.257e-26
Identity = 89/308 (28.90%), Postives = 141/308 (45.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF---IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSIS----QLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDV-TIDEGNAAKFIINIHSHPRA 1297
            P F   L+N   +NE       +++ P GD +L VEW ++ K +   +R +   + G+V LDI +  +ED GVY+C A +  G+  +  A++RV  EA +  +      I   H  +    P+  +     + P F++ L     + +G   H E  V P  D +LK +W  NG  + +GSR     D G+  LDI    PED GVY C A N  GEA SS +        K+ +KSN++   +     +  + +      + + F ++  Q   QAP F   LQ    + EG     ++     PRA
Sbjct: 2356 PVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTT-ASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAM-------KVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQ---QAPVFTTHLQSYDKLHEGQHV--LLEAQVEPRA 2650

HSP 15 Score: 112.464 bits (280), Expect = 6.243e-24
Identity = 100/386 (25.91%), Postives = 172/386 (44.56%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV----------DNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLN---ESSISQLKKFENNI--QEEINDAFRNTEDQEDDQAPSFDKGLQD-VTIDEGNAAKFIINIH--SHPRASIVWSINNEEVISDS-VNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            AP F   LEN   + E +     +++ PA D  L VEW  +G+PL  G+RF T  + G+V+L+I     EDSG Y C A ++ G+  +  AT+++  +  +          +  ++ I         +    PD        + +     ++ + +    H E  + P ND +++VDW  NG+++  GSR   + D G+  L+I   Y  D G+YTC A N  GEA  S  ++     + IV +  L     +    L+K E  +  +EE+      TED++ +  P F + ++D + + EG    F   +     P   I W  N   + + S V++    G I   ++     + G++  R  ATN  G   +   +T    +N
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPF--DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREEL-----VTEDEQPN-PPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCR--ATNKWGTATTSAKVTCKGKHN 3773

HSP 16 Score: 109.768 bits (273), Expect = 5.082e-23
Identity = 97/377 (25.73%), Postives = 166/377 (44.03%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF--IENQHVQQDENEPQYPDKHES-------SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINI--HSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVN 1480
            + PRF S+++    + +         + P  D  L VEW  +GK +    R  TV + GYVVLDI Y  + DSG YVC A +K G++ +     R T        V +  ++   +Q+   E + P   ++       ++ P F   +     + +G + H E  + P  D  L V+W+ NG+ +  G RF    D G   LDILY Y E+ GVY   A+N  GE + + + + C     ++  S L       L+K   N++  +      T ++   +AP F   L+++  + EG  A F   I     P+  + W  N   + + S  + + D     LE++     + G+++ R  A N  G+  +   + + 
Sbjct: 3128 QKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFT-----RTTLNCGGRGGVFYDSLQPDSLQRI-RELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGE-DVTRASLKCASKSSLILDSQLPRGMEGGLEKI-ANLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCR--AINEYGEAVTTATMKIQ 3494

HSP 17 Score: 108.227 bits (269), Expect = 1.647e-22
Identity = 91/372 (24.46%), Postives = 153/372 (41.13%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKFIINIH--SHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            PRF +   N   + E +     + + P GD S+ +EW  +GK L    R  T++  G V L++L +  ED+G Y C A +K G              A++  N+  ++                    Q P F+  ++    +  G + H E  + P ND  LKV+W+ NG+ M   +R   V D GY  LDI Y    D G Y C A N  GE + + + + C     +   S L   S+ ++++ E    ++ + +     +      P F   + DVT + EG +A F   +   + P   + W  N +++      + + D  I  L++  C E         A N  G+  +   L   S ++   D
Sbjct: 3029 PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGT-------------AEISCNLECVDKPR----------------GQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDS-LQPDSLQRIRELECPQGQQADTSAPLVAE-----PPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRASLKCASKSSLILD 3364

HSP 18 Score: 106.686 bits (265), Expect = 4.019e-22
Identity = 67/239 (28.03%), Postives = 114/239 (47.70%), Query Frame = 2 
Query:  386 EFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQN--------QSNPATVRVTPEAKVDNNVNFIEN--QHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCI 1072
            ++  P +   L+ E  ++E  PI L + V P  D +L +EW  +GK L  G+RF    E G+V +D++     D G+Y C A +K+G+          S    +  T   K    +  I++    +++D ++P+ PD       P F+ +  +   + +G   H E  + P  D ++ ++W  NG+ +    R   ++  G  AL++L T  ED G YTC A N  G AE S   + C+
Sbjct: 2888 QYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPD---LGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEIS-CNLECV 3122

HSP 19 Score: 88.5817 bits (218), Expect = 1.557e-16
Identity = 101/412 (24.51%), Postives = 165/412 (40.05%), Query Frame = 2 
Query:  293 NGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI 1516
             G       N V    R  G G +Q          P F++ +++  TV      R  ++V   G   L V WLK+G  +    +F  + E     L I+    EDSG Y CVA + +G             EA+ D +        + Q  ++P+ P    S ++P+  + L+S  TV +G  V     VD +   T +  W + G++    SR+  +  +G Y  L I   +PED+G Y CVA+N  G  ++S +Q+   P E      NL+                                AP     L+DV++ EG  A+F   +    +A+ V      ++I ++ +    F G+  +  +  T   + G   +RV ++   GQ+ S   LTV      A  L+ I
Sbjct: 4471 RGGTAKCSANLVVEERRRAGKGGIQ---------PPSFVTTIQS-TTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAG-------------EARCDGDC-------IVQSPSKPEKPTTPGSEKAPHIVEQLKSQ-TVEEGSKVIFRCRVDGKPTPTAR--WMR-GENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTS-AQLKVRPIE------NLD--------------------------------APPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSST--GQVESSAKLTVKKRRISAFQLRTI 4805

HSP 20 Score: 69.707 bits (169), Expect = 9.140e-11
Identity = 93/395 (23.54%), Postives = 154/395 (38.99%), Query Frame = 2 
Query:  338 GRPKGHGSV--QSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTED------QEDDQAPSFDKGL-QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF--YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            G  K  GSV    Q   ++ + PRF+  LE +     E  + +   +  +   S S +W    +P+ +      V +     L I     +  G + C A++  G   S  ATV + P+                         ++ E  +SP F ++L   + V  G A+ L   V  +   T KV+W+ N + ++         D +G C L I   +PE++G Y CVA N  G               K VSK+N+      +++ FE     EI     + ED        D+QAP   K L + +   EG  AK  + +   P+  + W  ++E++ +    +   + DGT + L +          I   A NPLG   +  +  V  I
Sbjct: 6819 GEAKSSGSVVVSDQKPDEQIKPPRFIQPLEPKYFGEHE--VAIIEAIVESEPLS-SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAENVGGSVTST-ATVNLIPQ-------------------------EEAEEFESPRFVEELVQPVEVMDGEALLLTCQVTGKP--TPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIG---------------KSVSKTNV------KIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAVEGI 7160

HSP 21 Score: 67.3958 bits (163), Expect = 5.199e-10
Identity = 93/394 (23.60%), Postives = 161/394 (40.86%), Query Frame = 2 
Query:  371 QYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDE------NEPQYPDKHES--SQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIV-SKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCT--EVGQHNIRVVATNPLGQMASDCILTVNSINNYAQD 1504
            Q E++EFE+PRF+  L   + V + + + L+ QV   G  +  VEW  + +  +T  + +T+ +  +G   L I     E+ G Y CVA +K G++ S               NV     +++   E      +E    D+  S   Q+P   K L   I   +G    LE  V   P+     KV W ++ + +     +    F+ G   L I + YP+D G  +  A N  G A ++         E IV SK       +SQ+        EE+  A +  +      +PS    ++D     G  AKF I    +P   I W  NN ++ +    +         LE+ + T  + G +N +++  N +G   +     ++S +   ++
Sbjct: 6933 QEEAEEFESPRFVEELVQPVEVMDGEALLLTCQVT--GKPTPKVEWYHNAE-KITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-------------TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKP----KVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTT----ALFAVEGIVGSKDYRKPEWVSQM--------EEMQVALKAAK-----CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLI--NEIGMTMTRAKFDISSTSTIVEE 7287

HSP 22 Score: 56.225 bits (134), Expect = 1.250e-6
Identity = 73/337 (21.66%), Postives = 115/337 (34.12%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            G+ + +V+W ++  P+            G  +L I     EDS V+ CVA+++ G              AK   N+   E +   +              Q P F   ++S  TV  G     +  V       L V W KNG  +    +F  + +     L I+  + ED G Y CVA N  GEA        C  D  + S S   + +    +K                       AP   + L+  T++EG+   F   +   P  +  W      V      +    G    L ++      +   + VA N LG + +   L V  I N
Sbjct: 4419 GEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGT-------------AKCSANLVVEERRRAGKG-----------GIQPPSFVTTIQST-TVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR-------CDGDCIVQSPSKPEKPTTPGSEK-----------------------APHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIEN 4698
[Close]
BLAST of Titin vs. UniProt/SwissProt
Match: sp|Q8WZ42|TITIN_HUMAN (Titin OS=Homo sapiens OX=9606 GN=TTN PE=1 SV=4)

HSP 1 Score: 108.227 bits (269), Expect = 1.392e-22
Identity = 113/468 (24.15%), Postives = 203/468 (43.38%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFE--RGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++         E  +G   L I  ++ +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D G+ TC A N  G   +S + I  + DEK +V +S L E    + ++++ E    E        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G +     L +    ++   L+  APE         P K    ++KVD          +P  T E  ++VK++R   +   H K P+  E+E+L  K
Sbjct: 1556 PMFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVD----------RPVDTTETKEVVKLKRAERIT--HEKVPE--ESEELRSK 2000

HSP 2 Score: 72.0182 bits (175), Expect = 1.967e-11
Identity = 87/391 (22.25%), Postives = 154/391 (39.39%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQ------------------------EDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G YT VA N  G+  SS + ++    E I+ +    E  + ++K+F ++      +   + E Q                        ++   P F K + +  I  G+ A   + +   P+  I W  N   +   +  KF  DG  + L +  T+  + G++    +A+N  G+      L +NS
Sbjct: 3237 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIITPLQDTVT-SEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLSLEAPESILHERIEQEIEM-EMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYT--CMASNDYGKTICSAYLKINS 3594

HSP 3 Score: 63.5438 bits (153), Expect = 7.816e-9
Identity = 87/351 (24.79%), Postives = 139/351 (39.60%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWL-KDGKPLLTGTRF--STVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNV-------------NFIE----------------NQHVQQDENEPQYPDKHESSQ---SPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAF-RNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVIS 1336
            P  ++ L++ +T +E  P R   +V+      L V W  KD K  +  +RF   T FE  Y  L+I  +  ED G Y  VA +  GQ  S        PE+ +   +             +F+                 N+ ++     P YP K +S +    P F K++ SN  ++ G    L   V   P+     K+ W  NG  ++  + +  VFD    +L IL+T  ED+G YTC+A N+ G       +  C    KI SK              E +   E   A  ++ E       P F K L+ +   +G  A F   +   P  ++ W   N+++ +
Sbjct: 3345 PAIITPLQDTVT-SEGQPARFQCRVS---GTDLKVSWYSKDKK--IKPSRFFRMTQFEDTYQ-LEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEV-SNADISMGDVATLSVTVIGIPKP----KIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYG-------KTICSAYLKINSKG-------------EGHKDTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAPTVTWFKENKQLCT 3663

HSP 4 Score: 63.1586 bits (152), Expect = 9.338e-9
Identity = 61/245 (24.90%), Postives = 111/245 (45.31%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSG----------------QNQSNPATVRVTPEAK--VDNNVNFIENQHVQQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S +V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G                + ++   T + T E K  V++    + +  + +++  P  P       +PYF +K +   +   +GG+V     V  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  943 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEP------AAPYFITKPVVQKLV--EGGSVVFGCQVGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSASASLL 1172

HSP 5 Score: 62.3882 bits (150), Expect = 1.918e-8
Identity = 65/283 (22.97%), Postives = 112/283 (39.58%), Query Frame = 2 
Query:  347 KGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSH--------------SQITCIPDEKIVSKSNLNESSISQLKKFENNIQ 1153
            K    V+  Y     + P  +      + V E+DP+ L   VA  G   L V+WLKDGK ++    FS  FE       I   +++DSG Y    ++  G +  + A +RV                    D+N P           P F+K L + +    G ++H+E  V     + +   W K+G+ +S  +++  V      +L++     ED   YTC   N  G+   S                +   IPD  +  K+ L  +   ++K F+++++
Sbjct: 6050 KNESGVERCYAFLLVQEPAQIVEKAKSVDVTEKDPMTLECVVA--GTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRVL-------------------DQNIP-----------PSFTKKL-TKMDKVLGSSIHMECKVS--GSLPISAQWFKDGKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGILTVKEPPSFLVKPGRQQAIPDSTVEFKAILKGTPPFKIKWFKDDVE 6296

HSP 6 Score: 61.2326 bits (147), Expect = 4.437e-8
Identity = 66/257 (25.68%), Postives = 99/257 (38.52%), Query Frame = 2 
Query:  305 VNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQS--------NPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKN-GQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES 1048
            +N  G +        G     S    KE + P         +TV+E + ++LS  V   G   + ++WLK G+ +    R S  F  G  VL++    + DSG YVC A + +G + +         PA    T +A VD  + F+         +EPQ     E + + + +K          GG            D    V W K   + ++ G R           L+I  T   D G+Y CVA N  GE ES
Sbjct: 9241 MNDAGLYTCKVSNDAGSALCTSSIVIKEPKKPPVFDQHLTPVTVSEGEYVQLSCHVQ--GSEPIRIQWLKAGREIKPSDRCSFSFASGTAVLELRDVAKADSGDYVCKASNVAGSDTTKSKVTIKDKPAVAPATKKAAVDGRLFFV---------SEPQSIRVVEKTTATFIAK---------VGG------------DPIPNVKWTKGKWRQLNQGGRVFIHQKGDEAKLEIRDTTKTDSGLYRCVAFNEHGEIES 9465

HSP 7 Score: 55.8398 bits (133), Expect = 1.648e-6
Identity = 64/250 (25.60%), Postives = 104/250 (41.60%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHE-SSQSPYFSKDL------ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDK 1222
            V W KDGK L     F   +   G   L I    E D G YVC    + G +++N   +    ++  +      E +   Q   E     K E  +  P  SK +      ++ ++ +      ++   +PR        W K+G++++ G ++    D+G   L+I  T   D G+YTC  KN+ G   SS    I  I D +    S    S I+  KK    +QEEI+     +E+ +  +A S +K
Sbjct: 33676 VTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAI----WTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKK--AVVQEEISQKALRSEEIKMSEAKSQEK 33919

HSP 8 Score: 55.8398 bits (133), Expect = 1.887e-6
Identity = 112/505 (22.18%), Postives = 195/505 (38.61%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDN--------NVNFIENQH---VQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITC-IPDEKIVSKSNL------------NESSISQLKKFENNIQEEINDAFRNTEDQEDD---QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTI 1762
            A R L+     +TV E +  R S      G+   +V WL+ G+ L T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +         V   E  H   V   E +P   +K +    S  P  ++ L++  +        L  +V+       +V W+K+G+ +    + N  F   Y A          DG Y  +  NN    ES   +  C I  E   SK+NL              S IS+ KK +    E  +   R TE +  +     P    GLQD T+   + AKF +     PR + +W+ + + +      K   D    +LE+  T  ++ G +   V   N  G ++S C LT+ +I +              +K+++ K  ++TP+    + +     E   K  R+ E+K    K+ + L  ++   KV  + + K + +T   ++I
Sbjct: 33487 AARILTK-PRSMTVYEGESARFSCDTD--GEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVK-SPEPRVKSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKE---IAKLTCVVESSVLRAKEVTWYKDGKKL----KENGHFQFHYSA----------DGTYE-LKINNL--TESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISETKKSDQKTTE--STVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTV--KNSAGSVSSSCKLTIKAIKDTEA-----------QKVSTQKTSEITPQKKAVVQE-----EISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSI 33947

HSP 9 Score: 54.6842 bits (130), Expect = 3.744e-6
Identity = 74/405 (18.27%), Postives = 150/405 (37.04%), Query Frame = 2 
Query:  479 AGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEA-------------------------------ESSHS-----QITCIPDEKIVSKS--------------NLNESSISQLKKF----ENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRC--TEVGQHNIRVVATNPLGQMASDCILTVNSIN---NYAQDLKHI 1516
            +G   +SV W +DG  +++G +  + F      L++      D+G+Y CVA + +G ++ +                       VQ+  +  Q PD                ++ V  G ++   +++  R     KV W K  + +  G   N   +     L++    P + G Y+C+  N+ G A                               E++++      ++ I DE ++S+S               + ES+I    ++    EN   ++I +A  +       + P F + L+ V    G  A     +   P   I W   + ++ S    K      +  L + +   ++VG+++ +  A N +G +AS  +L + +      +A+ LK +
Sbjct: 7692 SGSAPISVGWFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAVLT-------------------VQEPPSFEQTPD----------------SVEVLPGMSLTFTSVI--RGTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAGSASCTTHLFVKEPATFVKRLADFSVETGSPIVLEATYTGTPPISVSWIKDEYLISQSERCSITMTEKSTILEILESTIEDYAQYSCLIENEAGQDICEALVSVL-----EPPYFIEPLEHVEAVIGEPATLQCKVDGTPEIRISWYKEHTKLRSAPAYKMQFKNNVASLVINKVDHSDVGEYSCK--ADNSVGAVASSAVLVIKARKLPPFFARKLKDV 8052

HSP 10 Score: 54.299 bits (129), Expect = 5.912e-6
Identity = 60/234 (25.64%), Postives = 93/234 (39.74%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   +S+  P F K L +N    +G +V  E  V      TLK  W K+GQ +SLG     + +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 32648 VTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGED-SCKAKLTVT------------------------LHPPPTDSTLRPMF-KRLLANAECQEGQSVCFEIRVSGIPPPTLK--WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYKKQEFKSKEEHERHVQKQIDKTLRMAE 32844
[Close]
BLAST of Titin vs. UniProt/SwissProt
Match: sp|A2ASS6|TITIN_MOUSE (Titin OS=Mus musculus OX=10090 GN=Ttn PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.776e-21
Identity = 117/470 (24.89%), Postives = 199/470 (42.34%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSN-PATVRVT-----PEAKVDNNVNFIENQHVQQDENEPQYP--DKHESSQSPYFSKDLE---------SNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRN-----TEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPE--------NPYKKMASLKKVDVTPELTTALHKPKPTAE--KIVKIERNAEVKARHTKTPDVLEAEQLYDK 1705
            P F+  L+N   VN ++  RL  +V   G+ +  + WLK+   ++     R      RG   L I   I +DS  Y   A +K+G++ +     V V      PE K+         + +   E EP +    + +  +   + K+ +         +++ + + G  H E  + P  D T+ V+W  +G+ +   +R   + + GYC+LD    Y  D GV TC A N  G   +S + I  + DE    KS + ES +   KK    I+E    A        T DQ++ Q P      + V + EG  A+F   +  +P+  + W +N + +      +   DG I+YL++  C       ++V A NP G       L +    ++   L+  APE         P K    ++KVD          +P  T+E  ++VK++R   +   H K  +  E+E+L  K
Sbjct: 1562 PAFVEKLKN---VNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDE----KSLVEESQLPDGKKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRR-APEPKPEFHVHEPGKLQFEVQKVD----------RPVDTSETKEVVKLKRAERIT--HEKVSE--ESEELRSK 2006

HSP 2 Score: 77.411 bits (189), Expect = 4.502e-13
Identity = 89/388 (22.94%), Postives = 155/388 (39.95%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV-FDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPD----EKIVSKSNLNESSISQLKKFENNIQEEIND----------------AFRNTE--DQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            E P+ L  L+  +TV    P R  + ++  G     + W K+ + L TG +   + +     L ++ +  ED+ VY C AK+  G   ++ +     PE              V  D+  P YP        P     L+  +T ++G     +  V   +   LKV W+   + +     F    F+  Y  L+I   YPED+G Y  VA N  G+  S+ +     P+    E+I  +  +    I+ L   E + Q   +D                  R+T+   +++  AP F + + DV I   + AK  + +   P+  I W  N   +   +  KF  DG  + L +  TR  + G++    +A+N  G+      L ++
Sbjct: 3243 EPPQVLQELQ-PVTVQSGKPARFCAVIS--GRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEV-------------VSPDQEMPVYP--------PAIVTPLQDTVT-SEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQVSSTATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYT--CLASNEYGKAVCSAHLRIS 3599

HSP 3 Score: 62.3882 bits (150), Expect = 1.676e-8
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVN---------FIENQHV-------QQDENEPQYPDKHESSQSPYF-SKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            P  +S L+N +TV E + + L   ++  G  S  V W ++   + +   F   F+ G   L I  +  EDSG + C A +++G  + S    V+V+ E   +  +          F+E++ V        +++  P  P       +P+F SK +   +   +GG+V  E  +  +P+  V     W K+G  ++ G R+   +++  G C L I  T+ +D G YT V +N  GE  +S S +
Sbjct:  946 PTLVSGLKN-VTVIEGESVTLECHIS--GYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEP------AAPFFISKPVVQKLV--EGGSVVFECQIGGNPKPHVY----WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGETSASASLL 1174

HSP 4 Score: 55.0694 bits (131), Expect = 3.298e-6
Identity = 82/329 (24.92%), Postives = 142/329 (43.16%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA--KVDNNVNFIE--NQHVQQDENEP----QYPDKHES-SQSPYFSKDL-ESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHS-QITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFII--NIHSHPRASIVWSINNEEVISDS 1342
            P+   +L+ E +   +D  +L+  V  +   +  V W KDGK L     F   +   G   L I    E D G YVC     SG+  ++  + + T ++   +   V+ I    + VQ+    P    Q P   ES S  P     L ++ ++ +      ++   +P+  +T    W K+G++++ GS++    ++    L+IL T   D G+Y C   N+ G   SS    I  + D +    S    S ++  KK   + QEEI+     +E+ +  +     K  + + I E  A+K +I   +     AS+  SI +EEV   S
Sbjct: 34507 PKITQSLKAEAS---KDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEV---SGEGGTSKTSFQFTGQSFKSIHEQVSSISETTKSVQKTAESPEAKKQEPIAPESISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTIT----WTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKK--ASAQEEISQKALTSEEIKMSEV----KSHETLAIKE-EASKVLIAEEVKRSAAASLEKSIVHEEVTKTS 34818

HSP 5 Score: 54.299 bits (129), Expect = 5.526e-6
Identity = 84/411 (20.44%), Postives = 155/411 (37.71%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQ-----------------SNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQS--------------PYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----------HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLEL--TRCTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            A R L+     ITV+E +  R S      G+   +V WL++G+ + T  R      +     +I      D G Y  V ++  G+ +                 ++P  V+ +PE +V +       + V+  E    +P     +++              P  ++ L++  + +      L   V+       +V W+K+G+ +     F   +   G   L I      D G Y C      G +++S           H Q++ I +    +KS    +   + KK E    E I+              P    GL+D T+   + AKF I +   P+ +I W+ + + +   S  K   +     LE+  T  ++ G +   V  TN  G ++S C LT+ ++ +
Sbjct: 34349 AARILTK-PRSITVHEGESARFSCDTD--GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFTLTVQKARVIEKAVTSPPRVK-SPEPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKD---IAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTSFQFTGQSFKSIHEQVSSISE---TTKSVQKTAESPEAKKQEPIAPESIS------------SKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTV--TNSAGSVSSSCKLTIKAVKD 34735

HSP 6 Score: 53.9138 bits (128), Expect = 6.435e-6
Identity = 61/234 (26.07%), Postives = 94/234 (40.17%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTG---TRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD-RGYCALDILYTYPEDDGVYTCVAKNNCGEAES-SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTE 1186
            V W K G+ +  G    +++   ++G   L I     +D   Y  VA++K G++ S  A + VT                         +P   E++  P F K L +N   ++G +V  E  V      TLK  W K+GQ +SLG     V +   Y AL I  T PED G Y   A N  G     +H Q         V +    +      ++ E ++Q++I+   R  E
Sbjct: 33509 VTWYKSGQKIKPGDDEKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKHGED-SCKAKLTVT------------------------LHPPPTETTLRPMF-KRLLANAECHEGQSVCFEIRVSGIPAPTLK--WEKDGQPLSLGPHIEIVHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQ---------VERLRYVKQEFQSKEERERHVQKQIDKTLRMAE 33705
[Close]
BLAST of Titin vs. UniProt/SwissProt
Match: sp|O01761|UNC89_CAEEL (Muscle M-line assembly protein unc-89 OS=Caenorhabditis elegans OX=6239 GN=unc-89 PE=1 SV=3)

HSP 1 Score: 83.9593 bits (206), Expect = 4.694e-15
Identity = 101/399 (25.31%), Postives = 160/399 (40.10%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFE----------APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQ-NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR---GYCALDILYTYPEDDGVYTCVAKNNCGEAES---SHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQA--PSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGT--INYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            G    Q+ Y+ +  E          AP+    LE++ TVN   PIRLS +V        SV W KDG PL   +R S  +E  G   L I  S EED G Y CVA +  G  N S    V+V               Q V+++  EP            +F+K L  ++  ++G +  L+  V    D   ++ W++NGQ +  G R   V +    G C+L +  +   D+G+Y C A+N  G+A++   +H Q+                                   A   TE  + D+   P F   L D+++  GN       +   P  S+ WS +   +I DS  ++  + +  +  L +   T   +   R VATN  G   +   + ++
Sbjct: 5357 GQAETQATYQVEILEHVEEEKRREYAPKINPPLEDK-TVNGGQPIRLSCKVDAI--PRASVVWYKDGLPLRADSRTSIQYEEDGTATLAINDSTEEDIGAYRCVATNAHGTINTSCSVNVKVP-------------KQEVKKEGEEP------------FFTKGL-VDLWADRGDSFTLKCAV--TGDPFPEIKWYRNGQLLRNGPR--TVIETSPDGSCSLTVNESTMSDEGIYRCEAENAHGKAKTQATAHVQM-----------------------------------ALGKTEKPKMDEGKPPKFILELSDMSVSLGNVIDLECKVTGLPNPSVKWSKDGGPLIEDSRFEWSNEASKGVYQLRIKNATVHDEGTYRCVATNENGSATTKSFVRMD 5687

HSP 2 Score: 65.0846 bits (157), Expect = 2.470e-9
Identity = 91/381 (23.88%), Postives = 140/381 (36.75%), Query Frame = 2 
Query:  362 VQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER---GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFD--RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVI-SDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSI 1486
            V  Q   KE E P F   L + +  +  D   L   V   GD    ++W ++G+ L  G R  TV E    G   L +  S   D G+Y C A++  G+ ++  AT                   HVQ    + + P K +  + P F  +L S+++V+ G  + LE  V    + ++K  W K+G  +   SRF    +  +G   L I      D+G Y CVA N  G A                        +     + ++ +   +  A          Q P F   + DV   EG   K    + + P   +VW  +   V  SD +        +  L +  C        RV+ATNP G        TV  +
Sbjct: 5475 VPKQEVKKEGEEPFFTKGLVD-LWADRGDSFTLKCAVT--GDPFPEIKWYRNGQLLRNGPR--TVIETSPDGSCSLTVNESTMSDEGIYRCEAENAHGKAKTQ-ATA------------------HVQMALGKTEKP-KMDEGKPPKFILEL-SDMSVSLGNVIDLECKVTGLPNPSVK--WSKDGGPLIEDSRFEWSNEASKGVYQLRIKNATVHDEGTYRCVATNENGSA------------------------TTKSFVRMDDGLGSGVVTA---------SQPPRFTLKMGDVRTTEGQPLKLECKVDASPLPEMVWYKDGAIVTPSDRIQISLSPDGVATLLIPSCVYDDDGIYRVIATNPSGTAQDKGTATVKKL 5794

HSP 3 Score: 60.4622 bits (145), Expect = 7.027e-8
Identity = 89/372 (23.92%), Postives = 143/372 (38.44%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTR--FSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQ--SPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNA-VFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV-ISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            +E EAP F+  L+    V +  P  L  +V   G     V+W KDG P+    +       E G   L I  +   D G Y C A +K+G++++         E K+                  P+Y  + ++++   P F + L+    V +G  V LE  V+  +   +K  + KN Q + +G      V + G   L I     ED G Y C A N  G+A ++        D KI   + + E    +  + E   Q E      +++      AP F + L+  T+ E   A     +   PR  I W+   +EV +S  V   + D     L     T+      R  A N  G   ++  + V 
Sbjct: 3575 EEIEAPLFVQGLK-PYEVEQGKPAELVVRVE--GKPEPEVKWFKDGVPIAIDNQHVIEKKGENGSHTLVIKDTNNADFGKYTCQATNKAGKDET-------VGELKI------------------PKYSFEKQTAEEVKPLFIEPLKETFAV-EGDTVVLECKVNKESHPQIK--FFKNDQPVEIGQHMQLEVLEDGNIKLTIQNAKKEDVGAYRCEAVNVAGKANTNA-------DLKIQFAAKVEEHVTDESGQLEEIGQFETVGDTASSKTDTGRGAPEFVELLRSCTVTEKQQAILKCKVKGEPRPKIKWTKEGKEVEMSARVRAEHKDDGTLTLTFDNVTQADAGEYRCEAENEYGSAWTEGPIIVT 3908

HSP 4 Score: 58.151 bits (139), Expect = 3.017e-7
Identity = 79/373 (21.18%), Postives = 149/373 (39.95%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTV-FERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGS----RFNAVFD--RGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNK----FYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTV 1477
            +AP+ +  LEN I + E+   RL  + +  GD   +++W KDG+ +    R   +    G   L +  +  +D+G Y CVA++  G              A+   +VN I      +D +           ++P F+  L        G +V  E +  P  +    + W K+G  + L S    +  A  D  +     D+ +     +G + CVA N  G A S+ +++    D  I S+                    E+N        + ++  P   +GL +++I EGN  + I+     P  ++ W  + +E++ D  +     F  +  I++L +   +   +    + ATN LG   ++  L +
Sbjct: 5813 KAPKLMEPLEN-IRIPEKQSFRLRCKFS--GDPKPTIKWFKDGERVFPYGRLQLIESPDGVCELVVDSATRQDAGGYRCVAENTYGS-------------ARTSCDVNVIRGDRKPRDID-----SSIREGKAPGFTTPLTIR-RAKPGDSVTFECL--PFGNPFPSIKWLKDG--LELFSDEKIKMEAAADGTQRLILSDVTFL---SEGYFRCVATNEHGTA-STKAELVIEGDRTIGSRP-----------------LPEVNG-------EPEECKPRIRRGLYNMSIHEGNVVEMIVCATGIPTPTVKWYKDGQEIVGDGPDGKRVIFTDERGIHHLVIVNASPDDEGEYSLEATNKLGSAKTEGSLNI 6131

HSP 5 Score: 53.9138 bits (128), Expect = 7.161e-6
Identity = 63/233 (27.04%), Postives = 96/233 (41.20%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLT----GTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPR--NDVTLKVDWHKNGQSMSLGSRFN-AVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIP 1075
            PR    L N ++++E + + +   V   G  + +V+W KDG+ ++     G R     ERG   L I+ +  +D G Y   A +K G              AK + ++N I  +H+   +     P        P F + L++    N     H+ TI D         +V+W  NG+ +  G R        G  AL IL T  ED G Y   AKN+ G A SS      +P
Sbjct: 6038 PRIRRGLYN-MSIHEGNVVEMI--VCATGIPTPTVKWYKDGQEIVGDGPDGKRVIFTDERGIHHLVIVNASPDDEGEYSLEATNKLGS-------------AKTEGSLNIIRPRHIADADERGGMP------FPPGFVRQLKNKHVFN-----HMPTIFDCLVVGHPAPEVEWFHNGKKIVPGGRIKIQSCGGGSHALIILDTTLEDAGEYVATAKNSHGSASSSAVLDVTVP 6243

HSP 6 Score: 53.9138 bits (128), Expect = 7.663e-6
Identity = 66/251 (26.29%), Postives = 104/251 (41.43%), Query Frame = 2 
Query:  317 GNHVASFGRPKGHGSVQSQYE-SKEFEAPRFLSNL-ENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTR--FSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGS-----RFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEA 1042
            G + A    P G     ++ E ++  E P F+  L   E+ +NE   + ++ +  P      SVEWLKDG+P+ T +    + V   G   + I  +  EDSG Y C A        +NPA      EAK + N   ++N            P +     SP   K+ ES         + ++ +  P       V+W K+   +S+ +     +  AV   G  +L I      D G+Y+C A+N  GEA
Sbjct: 3258 GKYTAKASNPAGTAESSAEAEVTQSLEKPTFVRELVTTEVKINETATLSVTVKGVP----DPSVEWLKDGQPVQTDSSHVIAKVEGSGSYSITIKDARLEDSGKYACRA--------TNPAG-----EAKTEANFAVVKN----------LVPPEFVEKLSPLEVKEKEST-------TLSVKVVGTPEPS----VEWFKDDTPISIDNVHVIQKQTAV---GSFSLTINDARQGDVGIYSCRARNEAGEA 3467
[Close]
BLAST of Titin vs. UniProt/SwissProt
Match: sp|P11799|MYLK_CHICK (Myosin light chain kinase, smooth muscle OS=Gallus gallus OX=9031 GN=Mylk PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 5.810e-13
Identity = 90/365 (24.66%), Postives = 149/365 (40.82%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVD--PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF--YGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            P+F S  ++ +  +E   I+  S+V+  G     VEW K+G P+ TG       E G   L +  +   DSG Y C A +  GQ  ++       P+ +                              +P FS  L+   TV++G    L+  V   P  ++T    W  N Q +      ++ F+ G   L +    PEDDG+YTC+A+NN G A S  +Q+T      +  K +  ++  +Q  K        +N  F          AP F KGL D+ + +G+     + + ++P   I+W  N +E+       F   G+    Y++     + G++     A N LG+  +   LTV 
Sbjct:  429 PQFESRPQS-LEASEGQEIKFKSKVS--GKPKPDVEWFKEGVPIKTGEGIQIYEEDGTHCLWLKKACLGDSGSYSCAAFNPRGQTSTSWLLTVKRPKVE----------------------------EVAPCFSSVLK-GCTVSEGQDFVLQCYVGGVPVPEIT----WLLNEQPIQYA---HSTFEAGVAKLTVQDALPEDDGIYTCLAENNAGRA-SCSAQVT------VKEKKSSKKAEGTQAAK--------LNKTF----------APIFLKGLTDLKVMDGSQVIMTVEVSANPCPEIIWLHNGKEIQETEDFHFEKKGNEYSLYIQEVFPEDTGKYTCE--AWNELGETQTQATLTVQ 727
[Close]
BLAST of Titin vs. TrEMBL
Match: A0A504Y520 (Titin OS=Fasciola gigantica OX=46835 GN=FGIG_04181 PE=4 SV=1)

HSP 1 Score: 350.517 bits (898), Expect = 1.043e-103
Identity = 177/460 (38.48%), Postives = 278/460 (60.43%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF-----------IENQHVQQD-ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN--DAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKS 1726
            P+F+ +  ++ TVNE +P+ ++S+V PAGD+SL VEW K+GKPL   +RF+T F+RGY VLD LY+ E+D+G Y CVA +  GQ+QS+   + V PE  V  +              +ENQ +Q   +   +Y D+ + ++ P F K L   + + +    H ET+++P ND TL V+W+K+G+ +S+G RFNA+ DRG+  LDILY YPED+G Y C+AKN  G+ +S+   + C  +  IV+ S L++ S+S L+  +N   E++   D  +  E+QE   AP+FD     +T+ EG+ A+F++     PR  + W I+ E + S       + Y DG I++LE  R        IRVVA N LG+ A D +L +    ++  DL+H+ PENP++K+A LKKV+ + EL++A ++ +P A  + +IER +E+K R     + +EAE LY +V A L++
Sbjct:  422 PQFVQHPVSQATVNEGEPLSITSRVRPAGDSSLRVEWYKNGKPLAASSRFTTTFDRGYAVLDFLYTNEDDTGDYSCVATNNQGQDQSSGCRITVVPEEGVVTDTQLPEESMVASLAAMENQMMQNGLDRGSRYRDESKPTRPPTFLKPLNPQVGIRESTPAHFETLIEPANDATLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCIAKNALGQVQSNVVDLQCAGEANIVTSSVLSKESVSYLQSLDNWNSEQMAGYDTVK-VEEQETPAAPTFDILPVPITVTEGSPARFMVKAGGFPRPKVQWYIDGELLASQGGGGNWRIYQDGGISHLEFHRVGNPCTQQIRVVARNNLGESAVDTVLVIEPQVDFRPDLRHVEPENPFRKLAQLKKVERSNELSSAFNRTRPKALDLRRIERESEIKGRSVADAESIEAENLYTRVQAGLRT 880

HSP 2 Score: 110.153 bits (274), Expect = 5.494e-21
Identity = 97/332 (29.22%), Postives = 149/332 (44.88%), Query Frame = 2 
Query:  278 DSNLPNGNIVN---AQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV-------DNNVNFIENQHVQQDENEPQYP----DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQ---SMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD 1201
            D+ LP  ++V    A  N +   G  +G     S+Y  ESK    P FL  L  ++ + E  P    + + PA D +L+VEW KDGKP+  G RF+ + +RG+ +LDILY   ED+G Y C+AK+  GQ QSN   ++   EA +         +V+++++      E    Y     ++ E+  +P F   L   ITV +G       +V        KV W+ +G+   S   G  +    D G   L+              VA+NN GE+          Q+   PD + V   N     ++QLKK E +   E++ AF  T  +  D
Sbjct:  524 DTQLPEESMVASLAAMENQMMQNGLDRG-----SRYRDESKPTRPPTFLKPLNPQVGIRESTPAHFETLIEPANDATLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCIAKNALGQVQSNVVDLQCAGEANIVTSSVLSKESVSYLQSLDNWNSEQMAGYDTVKVEEQETPAAPTFDI-LPVPITVTEGSPARF--MVKAGGFPRPKVQWYIDGELLASQGGGGNWRIYQDGGISHLEFHRVGNPCTQQIRVVARNNLGESAVDTVLVIEPQVDFRPDLRHVEPENPFR-KLAQLKKVERS--NELSSAFNRTRPKALD 844
[Close]
BLAST of Titin vs. TrEMBL
Match: A0A4E0RJF1 (Titin OS=Fasciola hepatica OX=6192 GN=D915_002543 PE=4 SV=1)

HSP 1 Score: 347.821 bits (891), Expect = 9.699e-103
Identity = 175/460 (38.04%), Postives = 277/460 (60.22%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF-----------IENQHVQQD-ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN--DAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKS 1726
            P+F+ +  ++ TVNE +P+ ++S+V PAGD++L VEW K+GKPL   +RF+T F+RGY VLD LY+ E+D+G Y CVA +  GQ+QS+   + V PE  V  +              +ENQ +Q   +   +Y D+   ++ P F K L   + + +    H ET+++P ND +L V+W+K+G+ +S+G RFNA+ DRG+  LDILY YPED+G Y C+AKN  G+ +S+   + C  +  IV+ S L++ S+S L+  +N   E++   D  +  E+QE   AP+FD     +T+ EG+ A+F++     PR  + W I+ E + S       + Y DG I++LE  R        IRVVA N LG+ A D +L +    ++  DL+H+ PENP++K+A LKKV+ + EL++A ++ +P A  + +IER +E+K R     + +EAE LY +V A L++
Sbjct:  423 PQFVQHPCSQATVNEGEPLSITSRVRPAGDSTLRVEWYKNGKPLAASSRFTTTFDRGYAVLDFLYTNEDDTGDYSCVATNNQGQDQSSGCRITVVPEEGVVTDTQLPEESMVASLAAMENQMMQNGLDRGSRYRDESRPTRPPTFLKPLNPQVGIRESTPAHFETLIEPANDASLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCIAKNALGQVQSNVVDLQCAGEANIVTSSVLSKESVSYLQSLDNWNSEQMAGYDTVK-VEEQETPAAPTFDILPVPITVTEGSPARFMVKAGGFPRPKVQWYIDGELLASQGGGGNWRIYQDGGISHLEFHRVGSPCTQQIRVVARNNLGESAVDTVLVIEPQVDFRPDLRHVEPENPFRKLAQLKKVERSNELSSAFNRTRPKALDLRRIERESEIKGRSVADAESIEAENLYTRVQAGLRT 881

HSP 2 Score: 111.309 bits (277), Expect = 2.740e-21
Identity = 97/332 (29.22%), Postives = 149/332 (44.88%), Query Frame = 2 
Query:  278 DSNLPNGNIVN---AQGNHVASFGRPKGHGSVQSQY--ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV-------DNNVNFIENQHVQQDENEPQYP----DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQ---SMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD 1201
            D+ LP  ++V    A  N +   G  +G     S+Y  ES+    P FL  L  ++ + E  P    + + PA D SL+VEW KDGKP+  G RF+ + +RG+ +LDILY   ED+G Y C+AK+  GQ QSN   ++   EA +         +V+++++      E    Y     ++ E+  +P F   L   ITV +G       +V        KV W+ +G+   S   G  +    D G   L+              VA+NN GE+          Q+   PD + V   N     ++QLKK E +   E++ AF  T  +  D
Sbjct:  525 DTQLPEESMVASLAAMENQMMQNGLDRG-----SRYRDESRPTRPPTFLKPLNPQVGIRESTPAHFETLIEPANDASLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCIAKNALGQVQSNVVDLQCAGEANIVTSSVLSKESVSYLQSLDNWNSEQMAGYDTVKVEEQETPAAPTFDI-LPVPITVTEGSPARF--MVKAGGFPRPKVQWYIDGELLASQGGGGNWRIYQDGGISHLEFHRVGSPCTQQIRVVARNNLGESAVDTVLVIEPQVDFRPDLRHVEPENPFR-KLAQLKKVERS--NELSSAFNRTRPKALD 845
[Close]
BLAST of Titin vs. TrEMBL
Match: A0A183A5N1 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2266 PE=4 SV=1)

HSP 1 Score: 343.584 bits (880), Expect = 1.126e-100
Identity = 177/460 (38.48%), Postives = 275/460 (59.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF-----------IENQHVQQDENE-PQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN--DAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKS 1726
            P+F+ +   + TVNE +P+ ++++V PAGD+SL VEW K+GKPL   +RF+T F+RGY VLD LY+ E+D+G Y CVA +  GQ+QS+   + V PE  V                 +ENQ +Q   +  P+Y D+   ++ P F K L     + +    H ET+++P ND TL V+W+K+G+ +S+G RFNA+ DRG+  LDILY YPED+G Y CVAKN  G+ +S+   + C+ +  IV+ S L++ S+S L+  +N   +++   D  +  E++E   AP+FD     +T+ EG+ A+F++     PR  + W I+ E + S       + Y DG I++LE  R        IR VA N LG+ A D +L +    ++  DL+H+ PENP++K+A LKKV+ + EL++A ++P+P A  + +IER  E KAR     + +EAE LY +V A L++
Sbjct:  418 PQFIQHPIPQSTVNEGEPLSINARVRPAGDSSLRVEWYKNGKPLAASSRFTTTFDRGYAVLDFLYTNEDDTGDYSCVATNSQGQDQSSGCRITVIPEEGVVTETQLPEESMVASLAAMENQMMQNGMDRGPRYRDEGRPTRPPTFLKPLNPQTGIREAAPAHFETLIEPANDATLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCVAKNALGQVQSNVVDLQCVGEANIVTSSVLSKESVSYLQSLDNWSSDQMAGYDTVK-VEEEEAPAAPTFDILPVALTVTEGSPARFMVKAGGFPRPKVQWYIDGELLASTGGGGNWRIYQDGGISHLEFHRVGNPCTQQIRAVAKNNLGEAAVDTVLVIEPQADFRPDLRHVEPENPFRKLAQLKKVERSNELSSAFNRPRPQALDLRRIERENEFKARSAAEAESIEAENLYTRVQAQLRT 876

HSP 2 Score: 105.145 bits (261), Expect = 2.231e-19
Identity = 86/294 (29.25%), Postives = 129/294 (43.88%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV-------DNNVNFIENQHVQQDENEPQYP----DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQ---SMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD 1201
            E +    P FL  L  +  + E  P    + + PA D +L+VEW KDGKP+  G RF+ + +RG+ +LDILY   ED+G Y CVAK+  GQ QSN   ++   EA +         +V+++++      +    Y     ++ E+  +P F   L   +TV +G       +V        KV W+ +G+   S   G  +    D G   L+              VAKNN GEA          Q    PD + V   N     ++QLKK E +   E++ AF     Q  D
Sbjct:  553 EGRPTRPPTFLKPLNPQTGIREAAPAHFETLIEPANDATLTVEWYKDGKPVSMGCRFNAILDRGFAILDILYCYPEDNGAYWCVAKNALGQVQSNVVDLQCVGEANIVTSSVLSKESVSYLQSLDNWSSDQMAGYDTVKVEEEEAPAAPTFDI-LPVALTVTEGSPARF--MVKAGGFPRPKVQWYIDGELLASTGGGGNWRIYQDGGISHLEFHRVGNPCTQQIRAVAKNNLGEAAVDTVLVIEPQADFRPDLRHVEPENPFR-KLAQLKKVERS--NELSSAFNRPRPQALD 840
[Close]
BLAST of Titin vs. TrEMBL
Match: H2KSJ1 (Titin OS=Clonorchis sinensis OX=79923 GN=CLF_109481 PE=4 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 1.213e-98
Identity = 178/459 (38.78%), Postives = 279/459 (60.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN-----------NVNFIENQHVQQD-ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKS 1726
            P+F+ +   +I VNE +P  ++++V PAGD+SL VEW K+GKPL   +RF+ +F+RGY VL+ LY+ E+D+G Y CVA + SGQ+QS+   + V PE  V             N+  +ENQ +Q       +Y D+ +S++ P F K L     + +    H ETI++P ND +L+V+W+K+G+ +S+G RFNA+ DRG+  LDILY YPED+G Y C+AKN+ G+ +S+  ++ C P+  IV+ S L++ S+S L+  +N   E+++   R   ED+E   APSFD     +T+ EGN A+F++     PR  + W ++ E + S       + Y DG I++LE  R        +  VA N LG+ +++ +L +    ++  DL+H+ PENP+KK+A LKKV+ T EL +A ++P+P A  + +IER AE  AR     + LEAE LY +V + L++
Sbjct:  371 PQFVQHPVGQINVNEGEPTNVTARVRPAGDSSLRVEWYKNGKPLSASSRFNAIFDRGYAVLEFLYTNEDDTGDYSCVATNDSGQDQSSSCHINVIPEENVITDSQLPDESMIANLAAMENQMMQNGLARGTRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVVELQCSPEASIVTNSVLSKESVSYLQSLDNWGSEQMSGYDRPRIEDEEAPSAPSFDILPVAITVTEGNPARFLVKAGGFPRPRVQWYLDGELLASTGGGGNWRVYQDGGISHLEFHRVGPPCTQKVLAVARNNLGEASAETVLVIEPQIDFRPDLRHVEPENPFKKLAQLKKVERTSELNSAFNRPRPQALDLRRIERQAEYNARAAAENEALEAENLYARVQSQLRT 829

HSP 2 Score: 121.709 bits (304), Expect = 1.196e-24
Identity = 101/331 (30.51%), Postives = 153/331 (46.22%), Query Frame = 2 
Query:  278 DSNLPNGNIVN---AQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNN-------VNFIENQHVQQDE-----NEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQ---SMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD 1201
            DS LP+ +++    A  N +   G  +G    + + E K    P+F+  L  +  + E  P    + + PA D+SL VEW KDGKP+  G RF+ + +RG+ VLDILY   ED+G Y C+AK+  GQ QSN   ++ +PEA +  N       V+++++      E     + P+  D+ E+  +P F   L   ITV +G       +V        +V W+ +G+   S   G  +    D G   L+     P        VA+NN GEA +        QI   PD + V   N  +  ++QLKK E     E+N AF     Q  D
Sbjct:  473 DSQLPDESMIANLAAMENQMMQNGLARG---TRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVVELQCSPEASIVTNSVLSKESVSYLQSLDNWGSEQMSGYDRPRIEDE-EAPSAPSFDI-LPVAITVTEGNPARF--LVKAGGFPRPRVQWYLDGELLASTGGGGNWRVYQDGGISHLEFHRVGPPCTQKVLAVARNNLGEASAETVLVIEPQIDFRPDLRHVEPENPFK-KLAQLKKVERT--SELNSAFNRPRPQALD 793

HSP 3 Score: 118.242 bits (295), Expect = 1.530e-23
Identity = 77/240 (32.08%), Postives = 119/240 (49.58%), Query Frame = 2 
Query:  764 DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL-NESSISQLKKFENN-IQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCI 1468
            D  +S+  P F +     I VN+G   ++   V P  D +L+V+W+KNG+ +S  SRFNA+FDRGY  L+ LYT  +D G Y+CVA N+ G+ +SS   I  IP+E +++ S L +ES I+ L   EN  +Q  +    R  ++ +  + P F K L   T + E   A F  II   +     + W  + + V          D     L++  C          +A N LGQ+ S+ +
Sbjct:  363 DGLDSTGPPQFVQHPVGQINVNEGEPTNVTARVRPAGDSSLRVEWYKNGKPLSASSRFNAIFDRGYAVLEFLYTNEDDTGDYSCVATNDSGQDQSSSCHINVIPEENVITDSQLPDESMIANLAAMENQMMQNGLARGTRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVV 602
[Close]
BLAST of Titin vs. TrEMBL
Match: A0A3R7CUY5 (Titin OS=Clonorchis sinensis OX=79923 GN=sls PE=4 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 1.492e-98
Identity = 178/459 (38.78%), Postives = 279/459 (60.78%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDN-----------NVNFIENQHVQQD-ENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR-NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVN---KFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKS 1726
            P+F+ +   +I VNE +P  ++++V PAGD+SL VEW K+GKPL   +RF+ +F+RGY VL+ LY+ E+D+G Y CVA + SGQ+QS+   + V PE  V             N+  +ENQ +Q       +Y D+ +S++ P F K L     + +    H ETI++P ND +L+V+W+K+G+ +S+G RFNA+ DRG+  LDILY YPED+G Y C+AKN+ G+ +S+  ++ C P+  IV+ S L++ S+S L+  +N   E+++   R   ED+E   APSFD     +T+ EGN A+F++     PR  + W ++ E + S       + Y DG I++LE  R        +  VA N LG+ +++ +L +    ++  DL+H+ PENP+KK+A LKKV+ T EL +A ++P+P A  + +IER AE  AR     + LEAE LY +V + L++
Sbjct:  371 PQFVQHPVGQINVNEGEPTNVTARVRPAGDSSLRVEWYKNGKPLSASSRFNAIFDRGYAVLEFLYTNEDDTGDYSCVATNDSGQDQSSSCHINVIPEENVITDSQLPDESMIANLAAMENQMMQNGLARGTRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVVELQCSPEASIVTNSVLSKESVSYLQSLDNWGSEQMSGYDRPRIEDEEAPSAPSFDILPVAITVTEGNPARFLVKAGGFPRPRVQWYLDGELLASTGGGGNWRVYQDGGISHLEFHRVGPPCTQKVLAVARNNLGEASAETVLVIEPQIDFRPDLRHVEPENPFKKLAQLKKVERTSELNSAFNRPRPQALDLRRIERQAEYNARAAAENEALEAENLYARVQSQLRT 829

HSP 2 Score: 121.709 bits (304), Expect = 1.207e-24
Identity = 101/331 (30.51%), Postives = 153/331 (46.22%), Query Frame = 2 
Query:  278 DSNLPNGNIVN---AQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNN-------VNFIENQHVQQDE-----NEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQ---SMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-----HSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD 1201
            DS LP+ +++    A  N +   G  +G    + + E K    P+F+  L  +  + E  P    + + PA D+SL VEW KDGKP+  G RF+ + +RG+ VLDILY   ED+G Y C+AK+  GQ QSN   ++ +PEA +  N       V+++++      E     + P+  D+ E+  +P F   L   ITV +G       +V        +V W+ +G+   S   G  +    D G   L+     P        VA+NN GEA +        QI   PD + V   N  +  ++QLKK E     E+N AF     Q  D
Sbjct:  473 DSQLPDESMIANLAAMENQMMQNGLARG---TRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVVELQCSPEASIVTNSVLSKESVSYLQSLDNWGSEQMSGYDRPRIEDE-EAPSAPSFDI-LPVAITVTEGNPARF--LVKAGGFPRPRVQWYLDGELLASTGGGGNWRVYQDGGISHLEFHRVGPPCTQKVLAVARNNLGEASAETVLVIEPQIDFRPDLRHVEPENPFK-KLAQLKKVERT--SELNSAFNRPRPQALD 793

HSP 3 Score: 118.242 bits (295), Expect = 1.584e-23
Identity = 77/240 (32.08%), Postives = 119/240 (49.58%), Query Frame = 2 
Query:  764 DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL-NESSISQLKKFENN-IQEEINDAFRNTEDQEDDQAPSFDKGLQDVT-IDEGNAAKF--IINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCI 1468
            D  +S+  P F +     I VN+G   ++   V P  D +L+V+W+KNG+ +S  SRFNA+FDRGY  L+ LYT  +D G Y+CVA N+ G+ +SS   I  IP+E +++ S L +ES I+ L   EN  +Q  +    R  ++ +  + P F K L   T + E   A F  II   +     + W  + + V          D     L++  C          +A N LGQ+ S+ +
Sbjct:  363 DGLDSTGPPQFVQHPVGQINVNEGEPTNVTARVRPAGDSSLRVEWYKNGKPLSASSRFNAIFDRGYAVLEFLYTNEDDTGDYSCVATNDSGQDQSSSCHINVIPEENVITDSQLPDESMIANLAAMENQMMQNGLARGTRYRDEPKSTRLPQFVKPLNPQTGLRESAPAHFETIIEPANDSSLEVEWYKDGKPVSMGCRFNAILDRGFAVLDILYCYPEDNGAYWCMAKNSLGQVQSNVV 602
[Close]
BLAST of Titin vs. Ensembl Cavefish
Match: ENSAMXT00000044348.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:23245281:23271894:-1 gene:ENSAMXG00000032661.1 transcript:ENSAMXT00000044348.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 1.155e-20
Identity = 64/229 (27.95%), Postives = 106/229 (46.29%), Query Frame = 2 
Query:  773 ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNES----SISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLG 1447
            E  Q P+F K L S + + Q G VH E  + P  D T+ V+W  +G+ +   +R   V + GYC+LD    Y  D GV TC A N  G  ++S + +     + +V ++ L E      I ++K+  +     ++      E+  +   P      + V + EG  AKF   +  +P   + W +N + V+     +   DG I+YLE+  C       ++V+A NP G
Sbjct:   18 EKQQKPHFKKKLTS-VRLKQFGPVHFECRLTPIGDPTMIVEWLHDGKPLEAANRLRMVNEFGYCSLDYEAAYARDSGVITCRATNKFGIDQTSATLLV-REGKGLVEETQLPEGRSAQRIDEIKRLAHEGPSGVSG-----EEVTEKIKPEIVLLPEPVRVLEGETAKFRCRVTGYPAPKVNWYLNGQLVLKSKRFRLLFDG-IHYLEVVDCKSYDAGEMKVLAENPEG 238

HSP 2 Score: 69.3218 bits (168), Expect = 3.520e-12
Identity = 91/378 (24.07%), Postives = 144/378 (38.10%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQ-DENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLET-IVDPRNDVT----LKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV----ISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNY 1495
            + P F   L   + + +  P+    ++ P GD ++ VEWL DGKPL    R   V E GY  LD   +   DSGV  C A +K G +Q++ AT+ V     +       E +  Q+ DE +    +          ++ ++  I +       LE      R  VT     KV+W+ NGQ +    RF  +FD G   L+++     D G    +A+N                           E  I    KFE   +E+     R T            KG    T+  G  A F   +   P  S+ W  ++  +      + VN   G G +  + + +  + G ++ R  A+N  G+ +    L V    NY
Sbjct:   21 QKPHFKKKL-TSVRLKQFGPVHFECRLTPIGDPTMIVEWLHDGKPLEAANRLRMVNEFGYCSLDYEAAYARDSGVITCRATNKFGIDQTS-ATLLVREGKGLVEETQLPEGRSAQRIDEIKRLAHEGPSGVSGEEVTEKIKPEIVLLPEPVRVLEGETAKFRCRVTGYPAPKVNWYLNGQLVLKSKRFRLLFD-GIHYLEVVDCKSYDAGEMKVLAEN--------------------------PEGVIEHTVKFEVQQKEDFRTVLRQT------------KGPAPCTV--GGNAVFEYRVAGVPLPSVQWLKDSCHIQPGKFCNMVNNVDGSGFLKIINVQK-NDSGLYSCR--ASNLRGEASCSADLIVFLGTNY 352
[Close]
BLAST of Titin vs. Ensembl Cavefish
Match: obsl1b (obscurin like cytoskeletal adaptor 1b [Source:ZFIN;Acc:ZDB-GENE-050809-29])

HSP 1 Score: 74.7146 bits (182), Expect = 2.776e-13
Identity = 94/378 (24.87%), Postives = 160/378 (42.33%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVV-LDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMS---LGSRFNAVF-DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT-CIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDL 1507
            APRFL+     +  +  D + L  Q+   GD   +V W ++ + +  G R+  VFE G V  L I     EDSG Y+C AK+  G+  +  AT++V  EA+          +  Q++EN+P+           +  K L + +   +      +   +PR +V     W K+G+ ++     + F+  + D G+  L I  T   D GVYTC A+N  GE+ +    +    P  +   + N N  +    K  +   +     A R     +D+  P+  K ++   + EG  AKF   +   P+  I+W  +   ++S      Y D    + L++  C +         A+N  GQ  S   L+V     + Q L
Sbjct:    7 APRFLAYPRPVVVQSGTDAV-LKCQIG--GDPRPAVIWERNNEKIHPGGRYR-VFEDGNVYNLIITLVTAEDSGQYICKAKNSIGETYA-AATLKVEGEAQ----------EMEQREENKPR-----------FLIKPLSTRVGRGEDAVFSCKLWGNPRPEVA----WEKDGKKLNEIFESTHFSVGYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHE--DEGNRNGYTNGHWKGHQGKPRSGRPIAAR----LKDEPLPNSAK-VKMFAVTEGKHAKFRCYVTGKPKPEIIWRKDGRLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCAASNTAGQTLSAVHLSVKPPVRFKQPL 347

HSP 2 Score: 70.0922 bits (170), Expect = 7.846e-12
Identity = 74/274 (27.01%), Postives = 115/274 (41.97%), Query Frame = 2 
Query:  260 TNMNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPL---LTGTRFSTVFERG-YVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPAT-VRVTPEAKVDNNVNFIENQHVQQDENEPQ--YPDKHESSQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            T + +EDS        N+ G   A+    K  G  Q + E +E   PRFL    +      ED +  S ++   G+    V W KDGK L      T FS  ++ G +  L I  +   D GVY C A+++ G++ +     V   P  + + N N   N H +  + +P+   P        P  +        V +G        V   P+ ++     W K+G+ +  G R+    DR GY  L +LY   +D+GVY C A N  G+  S+
Sbjct:   68 TLVTAEDSGQYICKAKNSIGETYAA-ATLKVEGEAQ-EMEQREENKPRFLIKPLSTRVGRGEDAV-FSCKLW--GNPRPEVAWEKDGKKLNEIFESTHFSVGYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHEDEGNRNGYTNGHWKGHQGKPRSGRPIAARLKDEPLPNSAKVKMFAVTEGKHAKFRCYVTGKPKPEII----WRKDGRLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCAASNTAGQTLSA 332
[Close]
BLAST of Titin vs. Ensembl Cavefish
Match: OBSCN (obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF [Source:HGNC Symbol;Acc:HGNC:15719])

HSP 1 Score: 73.9442 bits (180), Expect = 5.205e-13
Identity = 106/431 (24.59%), Postives = 178/431 (41.30%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIE-EDSGVYVCVAKSKSGQNQSNPATVRV-TPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGL--QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALH--KPKPTAE-----KIVKIERNAEVK 1651
            APRFL+      +++      LS  V   G    +V W K    + TG RF T  E G V    +Y +  +DSG Y+C AK+  G+  +   T++V  PE  VD                 P +  K  SS+             V  GG V     V  R   T   +W K+G+ +   +R     D    +L I      D G YTC A+N  G A+++   +  + D +++S S+ + S +S L+K +  + ++I+  +R +           D G+  +   + EG  AK    +  HP+  I+W  +   +     +  Y D   N+ L++  C +         A+N  GQ  S  ++ V              P+ P+K+   L+ V+V  + T  L    P PT +     +  ++E+NA+ +
Sbjct:    9 APRFLTR-PKAFSLSVGRDATLSCTVV--GTPVPAVTWEKGKLHICTGGRFKTA-EDGDVYRLTIYDLTLDDSGQYMCRAKNTVGEAYA-AVTLKVGLPEPVVDRA---------------PAFTVKPVSSR-------------VGLGGDVTFFCRVAARP--TANFEWEKDGRYLGETNRIKITSDSESSSLRIQSIRSMDSGTYTCRAQNPIGRAQAAAVLVVDLQDTRLLS-SDKSTSLLSHLQKRKEEMCKDIS-LYRMS-----------DSGVFTRTCMVTEGKHAKLSCFVTGHPKPQIIWRKDRGNISEGRRHIMYEDQAENFILKILYCKQSDNGLYTCNASNMAGQTYSAVLVIVKE------------PKVPFKR--KLEDVEVKEKETAMLQCEVPGPTTQANWFMEETRLEQNAKYR 377
[Close]
BLAST of Titin vs. Ensembl Cavefish
Match: OBSCN (obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF [Source:HGNC Symbol;Acc:HGNC:15719])

HSP 1 Score: 73.1738 bits (178), Expect = 7.832e-13
Identity = 111/456 (24.34%), Postives = 181/456 (39.69%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIE-EDSGVYVCVAKSKSGQNQSNPATVRV-TPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDA----------------------FRNTEDQEDDQAPSFDKGLQDV-----TIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALH--KPKPTAE-----KIVKIERNAEVK 1651
            APRFL+      +++      LS  V   G    +V W K    + TG RF T  E G V    +Y +  +DSG Y+C AK+  G+  +   T++V  PE  VD                 P +  K  SS+             V  GG V     V  R   T   +W K+G+ +   +R     D    +L I      D G YTC A+N  G A+++   +  + D +++S S+ + S +S L+K +  + ++I+                         R T DQE   +    K L+ V      + EG  AK    +  HP+  I+W  +   +     +  Y D   N+ L++  C +         A+N  GQ              Y+  L  + P+ P+K+   L+ V+V  + T  L    P PT +     +  ++E+NA+ +
Sbjct:    9 APRFLTR-PKAFSLSVGRDATLSCTVV--GTPVPAVTWEKGKLHICTGGRFKTA-EDGDVYRLTIYDLTLDDSGQYMCRAKNTVGEAYA-AVTLKVGLPEPVVDR---------------APAFTVKPVSSR-------------VGLGGDVTFFCRVAARP--TANFEWEKDGRYLGETNRIKITSDSESSSLRIQSIRSMDSGTYTCRAQNPIGRAQAAAVLVVDLQDTRLLS-SDKSTSLLSHLQKRKEEMCKDISLYRMSDSAALSSSSSSRIVEDHVNLRLTTDQEQQVSSLTHKLLKGVFTRTCMVTEGKHAKLSCFVTGHPKPQIIWRKDRGNISEGRRHIMYEDQAENFILKILYCKQSDNGLYTCNASNMAGQ-------------TYSAVLVIVKPKVPFKR--KLEDVEVKEKETAMLQCEVPGPTTQANWFMEETRLEQNAKYR 413
[Close]
BLAST of Titin vs. Ensembl Cavefish
Match: obsl1a (obscurin like cytoskeletal adaptor 1a [Source:ZFIN;Acc:ZDB-GENE-060503-649])

HSP 1 Score: 70.0922 bits (170), Expect = 8.024e-12
Identity = 57/204 (27.94%), Postives = 86/204 (42.16%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFSTVFER----------GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPAT-VRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR-GYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            GD    V W KDGK L        ++E           G+  L I  +   D GVY C A +K G   +     V   PE + +N+ N   N H   ++++ +YP + + S+ P  +       TV +G        V   P+ ++     W K+G+ +  G R     DR GY  L +LY   +D G+Y C A N  G   S+
Sbjct:  180 GDPLPEVVWEKDGKQL------KEIYESAHFHIGQQDGGWFQLKIFRTRAPDGGVYTCKASNKHGTTVAGAVLLVEPVPEHRGENHQNGFTNGHWSPNQSKEKYP-RAKHSKEPQLNAAKAKKFTVTEGKHAKFRCYVTGKPKPEIV----WKKDGEPIEPGRRHLLFEDREGYYTLKVLYCKQQDCGLYICAASNALGNTLSA 372

HSP 2 Score: 55.4546 bits (132), Expect = 2.417e-7
Identity = 93/402 (23.13%), Postives = 154/402 (38.31%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRV--------------TPEAKVDNNVNFIEN-----QHVQQDENEPQYPDKH----ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMS---LGSRFN-AVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT-CIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQED-DQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLK 1510
            APR L      + V       L  Q+   GD    V W +   P++   R+    +     L I     ED+G Y+C AK+  G+  +  AT++V               PE + +  V   E      QH QQ +  P+ PDK     + ++  +  K L   +   +  A   +   DP  +V     W K+G+ +      + F+    D G+  L I  T   D GVYTC A N  G   +    +   +P+     +   +++  +      N  +E+   A  + E Q +  +A  F       T+ EG  AKF   +   P+  IVW  + E +     +  + D    Y L++  C +         A+N LG   S   L+V     + + L+
Sbjct:    7 APRVL-RYPRPVLVKRGSDALLRCQIG--GDPQPDVVWERKNVPIVPDGRYCIAQDGKVYTLAISGVTLEDAGQYICRAKNSIGETYA-AATLKVEEQSQEDQLEKPFQVPEVQQEVKVVPPEQEIKTFQHHQQIQPTPK-PDKEADLFQDNRPRFLIKPLSLRVDRGEDAAFSCKLWGDPLPEVV----WEKDGKQLKEIYESAHFHIGQQDGGWFQLKIFRTRAPDGGVYTCKASNKHGTTVAGAVLLVEPVPEH----RGENHQNGFTNGHWSPNQSKEKYPRAKHSKEPQLNAAKAKKF-------TVTEGKHAKFRCYVTGKPKPEIVWKKDGEPIEPGRRHLLFEDREGYYTLKVLYCKQQDCGLYICAASNALGNTLSAVHLSVKPPVRFKRGLQ 388
[Close]
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000826.1 (pep scaffold:Pmarinus_7.0:GL476876:251045:270583:1 gene:ENSPMAG00000000753.1 transcript:ENSPMAT00000000826.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 113.62 bits (283), Expect = 4.519e-26
Identity = 102/418 (24.40%), Postives = 181/418 (43.30%), Query Frame = 2 
Query:  359 SVQSQYESKE-FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGT--RFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNN----------------------VNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNES--SISQLKKFENNI------QEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLK 1510
            SV+   E+KE  E PRF+    N + V E   ++L+++    G+ +  + W ++   +       +     +G   L I  +   D   Y  +A +++G+     A  +V  EA + +                       +  +  +H Q   NE    DK E+ Q P+F K L S + + Q G VH E  + P  D  + V+W  +GQ +   +RF  V + GYC+LD    YP D G+ TC A N  G  ++S S I  + DEK +V +S L E      +L++ E  +      +E++ DA          + P F    +   + +G  A++   +   P+  ++W +N + +      +   DG I+YLE+  C       +++VA N  G +    +L +    ++   L+
Sbjct:  276 SVELVVEAKERLEKPRFVEK-PNNVCVKEGSAVQLTARAI--GNPTPDIVWQRNNDIIFPHKFPHYEVENTKGESTLTIYSAKPSDMAWYTIIALNRAGRTM---ARCKVQVEAMLSDRQPEPERKLIIPKGVYRDIAPPVLEPLHLRHGQDQWNEGDLYDK-ETQQRPFFKKKLTS-VRLKQFGPVHFECQMTPIGDPHMVVEWLHDGQPLQAANRFRIVCEFGYCSLDYEVAYPRDSGIITCRATNKFGCDQTSGSLI--VKDEKSLVEESQLPEGRRGFHRLQELEKGMAGTAVSKEDVPDA---------REKPYFVMYPEPCRVYDGETARYHCRVTGVPQPKVLWFLNGQRIHRSKRFRLRYDG-IHYLEIVDCKSYDTGEVKLVAENSEGSVECKAVLEILPKQDFRAVLR 673

HSP 2 Score: 50.447 bits (119), Expect = 1.671e-6
Identity = 64/275 (23.27%), Postives = 106/275 (38.55%), Query Frame = 2 
Query:  761 PDKHESSQSPYFSKDLESNIT---VNQG--GAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAV--FDRGYCALDILYTYPEDDGVYTCVAKNNCGEA--------ESSHSQITCIPDEKIVSKSNL-------NESSISQLKKFENNIQEEIN-------DAFRNTEDQ---EDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFY--GDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNS 1483
            P +H     P F   +ES +    V +G   A   +    P      K+ W+K+G+ +  G  +  V    +G   L I    PED+G+YT +A N  G A        E + +    +P     ++  +        + S SQL    N  Q  ++        A ++T  +   E    P F +  +  ++ EG  A+F + +   P     W  N ++V++D  +K     DGT   L +   T        VVA N  G  +    L V +
Sbjct:   14 PPRHSEDTGPSFEPAVESRLVSQRVQEGFPAAFFCQLSGHP----LPKILWYKDGRRIFTGPHYQLVGPTRQGEVELHIPSILPEDEGIYTVLACNASGNAVCSAKLSIEPAGNGAGGLPSPMSPARQQIPMPAWPGTQGSASQLPPPANRSQRPLSPERAEPVGALQDTSAEILVERLHKPMFVQKPESASVTEGQTARFDLRVVGRPMPDTFWFHNGKQVLNDQTHKVVVKEDGT-QSLIVVPATPADAGEWTVVAQNKGGSTSVSVELVVEA 283
[Close]
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002502.1 (pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-1 gene:ENSPMAG00000002253.1 transcript:ENSPMAT00000002502.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 2.972e-25
Identity = 129/499 (25.85%), Postives = 217/499 (43.49%), Query Frame = 2 
Query:  359 SVQSQYESKE-FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYV---VLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEA-------KVDNNVNFIENQHVQQDENEP---QYPDKH---------ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFR------NTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKK-----VDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEA-------EQLYDKV-AANLKSK 1729
            +VQ   ++KE    P F+  L N ++V E  P++LS  V  AG+    + WLK+ + +++  +F  +   G      L I  + + DS  Y   A +K+G++ +    V V  EA       K+  +    + + V   E EP   +Y  +          E  Q P F K L S + + Q G VH E  + P  D T+ V+W  + + +   +RF  V + GYC+LD    YP D G+ TC A N  G  ++S + I  + DE    KS + ES + + ++    IQE    A        +TE   +   P      +   + EG  A++   +  +P+  + W +N + +      +   DG I YL++  C       ++++A NP G+      + V    ++   L+  APE   + +A   K     V V  E   A  +P     K+ + ERN  +  + T+  D L +       E  Y+ + A  LKS+
Sbjct:  942 TVQLIVDAKEHLSRPHFVEKLRN-VSVKEGAPVQLS--VKAAGNPIPDIVWLKNSE-IISSQKFPNMRIEGATGESALSIDTTTQHDSAWYTSTAINKAGRDTTR-CKVNVEAEAFEPEPERKMVISKGIYKLKEVTAPELEPLHLRYGQEQWEEGDLYDKEKEQKPLFKKKLTS-VRLKQFGPVHFECRLTPIGDPTMVVEWLHDSKPLDAANRFRMVNEFGYCSLDYEVAYPRDSGIITCRATNKYGADQTSATLI--VKDE----KSLVEESQLPEGRRGIQRIQEMERQALEGGIVSVSTEGTLEKVKPEIVLFPEPARVLEGETARYRCRVTGYPQPRVSWYLNGQLIHKSKRYRLRYDG-IYYLDIVDCKSYDSGEVKLLAENPEGKAEHTVKIEVQLREDFRTLLRK-APEPKPEGLAEPGKPFFEVVKVKGEQAEAAGQPVEVV-KLKRTERN--IHEKQTEETDELRSKFKRRTEEGYYEAITAVELKSR 1423

HSP 2 Score: 71.633 bits (174), Expect = 5.604e-13
Identity = 62/230 (26.96%), Postives = 89/230 (38.70%), Query Frame = 2 
Query:  350 GHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQS-NPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            G  S  +  E KE + P          +V   +P  L   V   G     + WLKD K +  GT +   F     +L IL + + DSGVYVC A +++G + S  P T++                                E   +P F + LES + V  G    LE  V     V +KV W K+ + +     +  +F      L IL     D G+YTC A N+ G
Sbjct: 6136 GKISCAASLEVKERKNPPSFDQQLAPASVTAGEPADLGCHVT--GSPPFKISWLKDNKEIRPGTNYKFSFVDNTPLLTILKTDKRDSGVYVCRAANEAGYDTSCAPVTIK--------------------------------ERRVAPTFDQKLES-LNVTTGERAELECHVT--GSVPIKVTWAKDNREIRAAGNYRMIFMENIARLTILKVGKLDSGIYTCAASNDVG 6328

HSP 3 Score: 68.5514 bits (166), Expect = 5.048e-12
Identity = 57/212 (26.89%), Postives = 97/212 (45.75%), Query Frame = 2 
Query:  431 TVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAV-HLETIVDPRNDVTLKVDWHKNGQSMSLG--SRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            TV E + +R+  QVA  G  + ++ W ++   +     F   F+ G   L I  +  EDSG + C A +++G   S  + + V  E  +     F+E+       +E    D      +PYF +  +    V  G  V H +    P+  V     W K+G  ++ G   R+   +++  G C+L+I  T+ +D G YTCV +N  G+A +S
Sbjct:  387 TVVEGESVRMECQVA--GFPTPAIMWFREEYRIERSADFQIAFQAGVASLTIREAFAEDSGRFTCTATNEAG-TVSTSSYLLVQGEESI-----FLED-------SEASGTDTGGDGIAPYFVQKPQPQHLVEGGSVVFHCQIKGSPKPHVY----WKKSGIPLTTGYRRRYKVHYNKETGECSLEISMTFSDDAGEYTCVVRNKQGDAAAS 579

HSP 4 Score: 58.151 bits (139), Expect = 8.333e-9
Identity = 53/196 (27.04%), Postives = 79/196 (40.31%), Query Frame = 2 
Query:  449 PIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            P+RL  +V    D  ++V W K GK +    + S  F+    VL+IL S   D+G YVC A S++G ++S  ++V    E K D                             P   + L+  +     G VHL+  +  +    +KV W KN   +    R    F      L+I  +  ED   YTC A+N  G
Sbjct: 4266 PLRLECEVD--DDKGVTVAWTKSGKEIRPSRKLSFTFKNKLAVLEILKSELADTGDYVCTASSEAG-SESTASSV----EVKAD-----------------------------PAGLRALQPEVK-EAKGLVHLDCSL--KGSPVIKVTWFKNDNEVKADGRVQLSFVNNVAVLEIRDSETEDSADYTCEAQNEVG 4422

HSP 5 Score: 55.8398 bits (133), Expect = 4.095e-8
Identity = 83/336 (24.70%), Postives = 131/336 (38.99%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKD-GKPLLTGTRFSTVFERGYVVLDILYSIEE--DSGVYVCVAKSKSGQNQSNPAT-VRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHL-ETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYC--ALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEE------------------INDAFRNTEDQEDDQAPS-----FDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            EAP+ +   E+   +  +D +    +    GD   SV W K+ G+        ST+F +  V +  L ++    D+G Y CVAK+  G+ +   A  V +  E+ VDN V                      S   P F K L        G  + + E +  P  +V     W K+ + +   S  N V ++  C   L I    P D GVY C A N  GEA+ S + +  +P    V   N+  S ++Q    E ++ ++                  ++ A R  E    D  PS     F+  + DV +  G  A F   I + P A++ W
Sbjct: 2647 EAPQIIKATEDMFCIEGDDAM---FECLVFGDPEPSVTWAKEMGR--------STMFMKAGVDISWLSTLSRASDAGDYSCVAKNVVGEVECCSALFVEMPAESSVDNKV----------------------SETPPAFLKPLTGKKVYENGTLIFVCEVVGKPPPEVL----WMKDRRLIQPLS--NVVMEQEGCHYILQIKDVKPTDAGVYVCEATNFIGEAK-SFANVEVLP----VFDRNIATSPVTQHHFIEFDVDQDDLSRSPSPQEILLEGVQNVDLANREIEQVNIDVGPSETPPRFEGRIADVAVPIGAKAVFASRILAAPAANVAW 2938

HSP 6 Score: 53.9138 bits (128), Expect = 1.727e-7
Identity = 52/214 (24.30%), Postives = 87/214 (40.65%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            AP F       +   E   ++L S+V+  G   +++ W KD K L         F+    ++ +  +   DSG+YVC A     +N++  AT +VT        +N  E +H                  +P F K ++S +TV +G  +     V       L++ W K+ + +    + +  +  G C L+I      D G YTC A N  G
Sbjct: 6911 APMFTKKPLEILDEEEGKGVKLDSRVS--GSQPIAITWTKDDKDLHAHPGRELSFKNNIALMSLKSTKVSDSGLYVCKA-----ENEAGTATFKVT--------LNIKERKH------------------APNFDKKVQS-LTVVEGDTLSASCRVS--GSEPLQIMWLKDRKEVQPFGKVSIAYSEGDCTLEISQVTKADAGEYTCKAVNEAG 7088

HSP 7 Score: 52.373 bits (124), Expect = 4.811e-7
Identity = 65/311 (20.90%), Postives = 114/311 (36.66%), Query Frame = 2 
Query:  428 ITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVT--LKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKF 1354
            + V+E   +RL  +VA  G   +   W KD   + +G     V +    +L++      +SG YVC A++  G + ++ A + VT                              E    P F++ L+ ++   +GGA     + D R   T    V W K+G+ ++ G ++   F+     L+IL     D+  Y+C   N+ G       +I+C                                    + E +E    PSFD+ L   ++  G  A    ++   P   I W  +N+E+   +  KF
Sbjct: 5970 VKVSEPGSVRLECEVA--GTPVIKTSWFKDDVLITSGLNRKPVQKLNLAILELRNVQYSESGEYVCKAENDIG-SATSVAMLTVT------------------------------ERKNPPVFTRKLK-DVQGVEGGAA----MFDCRATGTPPFAVAWFKHGKEITPGEKYRMSFEENVVFLEILNLATNDEEEYSCTVSNDVG-------KISCAA----------------------------------SLEVKERKNPPSFDQQLAPASVTAGEPADLGCHVTGSPPFKISWLKDNKEIRPGTNYKF 6201
[Close]
BLAST of Titin vs. Ensembl Sea Lamprey
Match: mylka (myosin, light chain kinase a [Source:ZFIN;Acc:ZDB-GENE-030131-8207])

HSP 1 Score: 63.5438 bits (153), Expect = 1.456e-10
Identity = 57/206 (27.67%), Postives = 91/206 (44.17%), Query Frame = 2 
Query:  482 GDNSLSVEWLKDGKPLLTGTRFST-VFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENE------PQYPDKHESS---QSP--YFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQI 1063
            G+    V W K+G+PL  G  F      RG   L+++   E+DSG Y+C A++++G  Q       +T E KV+ +    + +  ++DEN       P  P +   S   +SP  + +K   + +   QGG  + +    P+  VT    W K    +     F          L++L    ED GVYTC   N+ G+A +S   I
Sbjct:    4 GNPEPQVLWFKNGQPLAAGEHFRVERSSRGAFSLEVVGLGEDDSGKYLCEARNEAGTRQ-------ITVELKVEGD----DRRLTRRDENTAACSGFPIPPVESRPSIWGESPPKFVNKPSRAVVREGQGGKFNCKITGRPQPQVT----WRKGDTELHNDGHFKVYEKSSLHFLEVLDVKEEDSGVYTCTITNSAGKASASAELI 194

HSP 2 Score: 60.077 bits (144), Expect = 1.759e-9
Identity = 85/340 (25.00%), Postives = 128/340 (37.65%), Query Frame = 2 
Query:  497 SVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLET--IVDPRNDVT-LKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV--ISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQ 1501
            +VEW KDG  + T  R+S    +G   L I    + D G + C AK+  G+  +   T+ V      D                           Q+P FS  L +  TV +     LE   +  P   VT LK D   N   MS           GYC L +     +D GVYTC+A+N+ G+A  S + +T     +  S+ N  + ++ Q                           P F KGL D+ + +G+     + + + P A IVW  N  E+    D V +   D    +++     + G +     A N  GQ  S  +LTV    +  Q
Sbjct:  369 AVEWSKDGVVIKTSERWSVEENQGGHQLIIFDVQKYDGGAFACAAKNSDGKATTATFTLIVKGVTDAD---------------------------QAPGFSAPLVA-CTVEEQQEFTLECHLLGKPEPSVTWLKDDKPVNSAVMSCRD--------GYCTLYVGAAGLDDGGVYTCIAENHLGKAYCS-ATVTVREPRRRTSRRNEEKPAVKQ-------------------------SPPVFVKGLCDLQVMDGSEVHLTVKVAAIPPAEIVWLHNGNEIKESEDFVLESKDDEHTLHIQEVFPEDTGCYTCE--AWNEAGQAQSQALLTVQEPQDGVQ 644

HSP 3 Score: 50.447 bits (119), Expect = 1.519e-6
Identity = 44/208 (21.15%), Postives = 83/208 (39.90%), Query Frame = 2 
Query:  869 RNDVTLKVDWHKNGQSMSLGSRFNA-VFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSIN 1489
            R +   +V W KNGQ ++ G  F      RG  +L+++    +D G Y C A+N  G  + +  ++    D++ +++ + N ++ S         +  I            +  P F        + EG   KF   I   P+  + W   + E+ +D   K Y   ++++LE+    E          TN  G+ ++   L +   N
Sbjct:    3 RGNPEPQVLWFKNGQPLAAGEHFRVERSSRGAFSLEVVGLGEDDSGKYLCEARNEAGTRQIT-VELKVEGDDRRLTRRDENTAACSGFPIPPVESRPSI----------WGESPPKFVNKPSRAVVREGQGGKFNCKITGRPQPQVTWRKGDTELHNDGHFKVYEKSSLHFLEVLDVKEEDSGVYTCTITNSAGKASASAELIIQISN 199
[Close]
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004663.1 (pep scaffold:Pmarinus_7.0:GL479759:1154:24713:-1 gene:ENSPMAG00000004240.1 transcript:ENSPMAT00000004663.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.151 bits (139), Expect = 6.994e-9
Identity = 46/175 (26.29%), Postives = 78/175 (44.57%), Query Frame = 2 
Query:  518 GKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEA 1042
            G+P+ +G R     E+G   L + ++  ED G Y   A +  G+   +   +   P A   +N++  +   +     EP+  +  E   +P FS+ L  ++ V +GG   LE     R  +  +V W + G+ + LG R  +  + G   L I      D GVY C+  N+ G A
Sbjct:    1 GRPVQSGERRRVSSEKGLHCLSVPHASAEDEGEYTVTAANTHGEVSCSCTLIVSEPRAPHASNIS--KRDMMPSIAEEPELGEA-ERDAAPDFSQPL-CDVAVLEGGCAVLECRGQAR--IPPQVSWLRQGEQVPLGGRVTSKEENGTFRLIIQSAKLADAGVYKCILSNSAGRA 169
[Close]
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49553 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL7])

HSP 1 Score: 78.5666 bits (192), Expect = 3.276e-15
Identity = 97/393 (24.68%), Postives = 154/393 (39.19%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES------SHSQITC-------IPDEKIVS-------KSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDD-QAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTR--CTEVGQHNIRVVATNPLGQMASDCILTVNSINN 1492
            K+ + P F+S LE  I + E D  +LS  V   G     V+W +D   +    RF   ++     L +  +  +D GVY CV  +  G   S               +V  ++     + +  P + +K        F + LE  I V Q   V       P+  VT    W KNG+ +  G          Y  L IL +  ED G Y C+A+N+ G AE           +T        +PD +  +       K  L +  I    ++E      I  AF   E   +  +AP F   L+ + + EG+   ++  +   PR ++ W  +N+ + +D+  K   DG +  L L+   C + G +N   V TN  G   S   L V+   N
Sbjct:   11 KKGDTPTFMSKLE-PIEITEGDDFQLSCNVT--GRPQPEVQWFRDEVLIKPSRRFKASYDGNVSALTVKEAELDDDGVYKCVVTNNFGSVSSKA-------------DVTVLKKHLRPERDYAPIFEEKQ-------FLEPLE--IQVRQPVEVSYHVRGRPKPTVT----WWKNGKELRDGKHVEIRSGGTYHTLYILRSEEEDAGSYKCLARNDVGSAERLFEVQVEEPSVTWYRNKNEIVPDGRSRTTFDGETYKLILQDIKIKDAGQYECIASNPIGKAFCTAEMTVNKREAPVFVSKLRPLFVTEGDNVSWMCKVAGMPRPTVEWYRDNQPLRADTRWKMDFDGRVCSLFLSEADCKDKGVYN--CVITNSAGSAFSSAELQVDKKAN 372

HSP 2 Score: 61.6178 bits (148), Expect = 6.892e-10
Identity = 82/332 (24.70%), Postives = 125/332 (37.65%), Query Frame = 2 
Query:  344 PKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSF---DKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV 1330
            P G     ++    + EAP F+S L   + V E D +    +VA  G    +VEW +D +PL   TR+   F+     L +  +  +D GVY CV  + +G   S       + E +VD   N            +P+  +K    Q+    +  E+   V   G         PR +VT    W++N   +   SRF+   D    +L I  T   D G Y CVA N  G               KI S+ +L+                           +E   AP F   D+  + V + E +A  F + +   P  +IVW  + E V
Sbjct:  260 PIGKAFCTAEMTVNKREAPVFVSKL-RPLFVTEGDNVSWMCKVA--GMPRPTVEWYRDNQPLRADTRWKMDFDGRVCSLFLSEADCKDKGVYNCVITNSAGSAFS-------SAELQVDKKAN------------KPKVIEKMNDVQT---VEGTEARFDVRFSGL--------PRPEVT----WYRNSIKVRNESRFHLSEDGDRYSLTITDTKLVDTGTYKCVATNEGG---------------KITSRGDLDV--------------------------RERQFAPEFYGDDELKEPVVVQESDAINFKVRVRGKPVPTIVWYKDGEMV 513
[Close]
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49552 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL6])

HSP 1 Score: 65.4698 bits (158), Expect = 3.631e-11
Identity = 94/417 (22.54%), Postives = 159/417 (38.13%), Query Frame = 2 
Query:  317 GNHVASFGRPKGHGSVQSQYESKEFE-APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVV-LDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQY---------PDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT--------CIPDEKIVSKSNLNESSISQ---------LKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFI-INIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVN 1480
            G +      P G  +++ +   KE + AP FL    ++   +      ++      G  +  + W KDG+PL   TR   +  RG V  L IL    ED+G Y C A ++ G    +   V VT   K   +V  +  +          +         P   +    P FS  L    T+        + +V+   +    V+W K+   +    RF   FD   C L    T  +D G+Y CV +N+ G   SS + I          +P  K V     +E+ +           ++ F  + +  + D  R   +Q D+    +   + ++ +++    K I  N H    + + W  N++ V  D + + Y DG +  L + R T       R    N LG  +S   L+VN
Sbjct:   13 GTYRCVVHTPAGQTTIRGELHIKEKQQAPLFLDEKVSQEKFSLPQGQEMNKSFTVTGSPTPHITWYKDGEPL-DDTRRIDIRSRGNVYYLGILNLKPEDAGTYTCEAGNRIGAAYRS-FEVDVTATLKRAEHVLLLSGRGSAMFMTTLLHIPLPFLSTVPKPKDKWSLPAFSSKLRPLETLE---GEETKFVVNVSGEPIPTVEWFKDDTPIKHEDRFRTYFDGRVCMLVFRKTGLDDQGIYKCVLRNDVGTVSSSANLIVSKKSASPQVVPKIKFVDSFECSEARLEAHVAGVPRPTVEWFLGSAK--LCDGERYRLEQTDNL---YTLVISNLHMEDTGPYKIIATNEHGSTGSVVEWFKNDKPVKHDHLVRSYFDGKVIRLIVRRTTLEDAGLYRCTILNDLGTASSMADLSVN 419
[Close]
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49558 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGM2])

HSP 1 Score: 63.5438 bits (153), Expect = 1.578e-10
Identity = 55/191 (28.80%), Postives = 82/191 (42.93%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT 1066
            VEWL+DG+P+  G RF   FE+   VL I+ +   D G Y CVA SK+G++         + E  VD                        E+   P F + + SNI + +G     +  +   P  +VT    W+K+   +  G RF    D    +L I      D G Y+C A N  G+A S + ++T
Sbjct:  391 VEWLRDGRPIKPGRRFQIEFEKDRSVLTIIDAKLNDEGEYTCVATSKAGKDSC-------SVELLVD------------------------EAVVPPEFVRKI-SNIELTEGEIARFDVRITGTPAPEVT----WYKDEVPIEDGGRFEIYSDDDKHSLVIRDCALSDTGSYSCKATNEAGQA-SCYGELT 544

HSP 2 Score: 57.3806 bits (137), Expect = 1.343e-8
Identity = 84/390 (21.54%), Postives = 155/390 (39.74%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPL--LTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNN-----------------------C-GEAESSHSQITCIPDEK----IVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTV 1477
            P F S L + I VNE + + L   V   G     V+W KDGKP+  L   RF          L I  +   D+G Y+C A + +G+  S+              NV+ +   HV      P+ P        P F ++L+ +IT+ +G +   +  V    +   KV W+ NG+ +      + V D  + +L +++   +  G YTCVA+N                        C G+    + ++  + D      ++  + L++ ++   K   +  + E +     T    D+  P F + L+   + EG+     + +   P+  + W  + E V   S+     +G ++ L +   T   +     +A N +G+++    L V
Sbjct:    3 PEFKSALRD-IEVNEGNTVTLRIHVT--GSPKPEVDWFKDGKPVSGLPHRRFEVGCVGEVYTLVIKEARGSDTGKYLCKAYNSAGEVTSS-------------CNVDVL---HVA-----PKEP--------PKFERNLK-DITIAEGCSTRFDVQVTGSPEP--KVFWYHNGKLVQPRDHVDIVHDDEHSSLILMHCPLDLSGKYTCVAENEVENDAVRLEVEVTGEPKPVVLWFCEGKPIEENRRVYTMRDGSTSVLVIENAELDDEAMYTCKAINDLGEVECSSEMLPT----DEAGPKFLQRLELKEVVEGSRTDLEVEVSGKPKPVVEWLKDGELVKPSSLMTLETEGQVHTLTIMNTTLDDEGEYTCIAKNKVGEVSCTAELLV 353
[Close]
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49550 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL4])

HSP 1 Score: 61.6178 bits (148), Expect = 3.268e-10
Identity = 60/215 (27.91%), Postives = 93/215 (43.26%), Query Frame = 2 
Query:  398 PRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFS--TVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            P F   L  ++ V E D  R   +V   G     VEW      +L   RF   +V E GY  L +   ++ED+G+Y C+A +K+G+     +T R   E                   NE Q+  + E+ + P   + L+   T+ +G A+ +E  V  + + T  + W K+G      +R    FD   C L I      D+G+Y C AKN+ G
Sbjct:  131 PEFKEKL-TKVEVTEGD--RAKFEVRVKGYPYPEVEWYHGSNRILDEDRFEIESVKETGYYSLTVGEVVQEDAGMYKCIAVNKAGK-----STCRADLEV------------------NERQFAPEFETEE-PTILQRLQPVDTI-EGEAIKMECRVSGKPEPT--ITWLKDGLVFLTDNRIKTYFDGDVCTLTIRDVKLGDEGIYRCNAKNSSG 315

HSP 2 Score: 52.373 bits (124), Expect = 3.038e-7
Identity = 81/354 (22.88%), Postives = 142/354 (40.11%), Query Frame = 2 
Query:  407 LSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER-GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHES---SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRF--NAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNI-RVVATNPLG 1447
            + N+E  I  +    I++     PA      V + ++ + +  G R+S V E  G  VL I  +  ED+G Y C+A + +G+  S    + V  E  +   ++    +   +++        H +   +  P F + L + + V +G     E  V  +     +V+W+     +    RF   +V + GY +L +     ED G+Y C+A N  G++       TC  D ++      NE   +   +FE                    + P+  + LQ V   EG A K    +   P  +I W  +    ++D+  K Y DG +  L + R  ++G   I R  A N  G
Sbjct:    7 MKNVEGRIGEDVRFEIQVEGTPVPA------VTFHRESEQIYDGGRYSIVEEENGLFVLTIRNTRPEDAGEYQCIAVNGAGEVTSK-GYLHVEEELTIPQFIDMGTYKCQVRNDLGRTSTSAHLNIIVATRPEFKEKL-TKVEVTEGDRAKFEVRV--KGYPYPEVEWYHGSNRILDEDRFEIESVKETGYYSLTVGEVVQEDAGMYKCIAVNKAGKS-------TCRADLEV------NERQFA--PEFET-------------------EEPTILQRLQPVDTIEGEAIKMECRVSGKPEPTITWLKDGLVFLTDNRIKTYFDGDVCTLTI-RDVKLGDEGIYRCNAKNSSG 315
[Close]
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49549 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL3])

HSP 1 Score: 58.5362 bits (140), Expect = 2.046e-9
Identity = 65/309 (21.04%), Postives = 118/309 (38.19%), Query Frame = 2 
Query:  383 KEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            K  E P F+  L ++  + E++  R   +V   G    + EWL+  KP+  G R++ + +     L+I     ED G+Y CV+ + SG + +   T R+T EA                             +  P F+ +L  +  V +G  + L+  + P+     K++W K+ + +    RF    +     L I     +D+G++ CV  N+ G A       +C  +  +      +  SI  L+                           F + L+D  + +G+   F + I   P   +VW
Sbjct:   13 KRGEPPEFIRKLRDQF-IQEKETARFVCRVI--GRPDPTYEWLRGDKPITDGGRYTIMSDDEGQTLEITDCTVEDEGLYKCVSTNSSGTDTT---TARLTVEA----------------------------PAPKPVFASEL-IDKQVKEGENLSLDVKL-PQEYKAEKIEWFKDNELIRDRPRFTVTSEDTRHTLTIATATFDDEGIFKCVLLNDFGIA-------SCSAEILVEGMRRRSRDSICALE---------------------------FQQELKDREVLDGDTIHFDVRITGRPEPKVVW 251
[Close]
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000027090.1 (pep primary_assembly:ASM223467v1:21:27247530:27253950:1 gene:ENSORLG00000024593.1 transcript:ENSORLT00000027090.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.8853 bits (211), Expect = 4.015e-18
Identity = 62/233 (26.61%), Postives = 103/233 (44.21%), Query Frame = 2 
Query:  836 GAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFE-NNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPEN 1528
            G  H E  + P  D T+ V+W  + + ++  +R   V + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V ++ L E      +  E   +  E   A  + +++ +   P      +   + EG+ A+F   +  +P   + W +N + +      +   DG I YLE+          +RVVA NPLG       L +    ++   L+  APE 
Sbjct:    5 GPAHFECRLTPIGDPTMVVEWLHDSKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRASNKFGVDQTSATLI--VKDEKGLVEETQLPEGRKGAYRVDEIERMVHEGGPAGVSADEESEKTKPEIVLLPEPARVLEGDIARFRCRVTGYPAPKVNWYLNGQLIRKSKRYRLNYDG-IYYLEIVDTKSYDSGEVRVVADNPLGTSEHTVKLEIQQREDFRSVLRR-APEQ 233

HSP 2 Score: 69.3218 bits (168), Expect = 1.607e-12
Identity = 66/213 (30.99%), Postives = 92/213 (43.19%), Query Frame = 2 
Query:  449 PIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQH--VQQDENEPQYPDKHESSQSPYFSKDLESN------ITVNQGGAVHLETIVDPRNDVT----LKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS 1051
            P     ++ P GD ++ VEWL D KPL    R   V E GY  LD   +   DSGV  C A +K G +Q++ AT+ V  E  +       E +    + DE E      HE   +   S D ES       + + +   V    I   R  VT     KV+W+ NGQ +    R+   +D G   L+I+ T   D G    VA N  G +E +
Sbjct:    6 PAHFECRLTPIGDPTMVVEWLHDSKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRASNKFGVDQTS-ATLIVKDEKGLVEETQLPEGRKGAYRVDEIERMV---HEGGPAG-VSADEESEKTKPEIVLLPEPARVLEGDIARFRCRVTGYPAPKVNWYLNGQLIRKSKRYRLNYD-GIYYLEIVDTKSYDSGEVRVVADNPLGTSEHT 212
[Close]
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000046443.1 (pep primary_assembly:ASM223467v1:21:968974:990901:1 gene:ENSORLG00000030355.1 transcript:ENSORLT00000046443.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 76.6406 bits (187), Expect = 4.251e-15
Identity = 63/217 (29.03%), Postives = 91/217 (41.94%), Query Frame = 2 
Query:  449 PIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKD-----------LESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQIT 1066
            P     ++ P GD ++ VEWL DGKPL    R   V E GY  LD   +   DSGV  C A +K G +Q++ AT+ V  E  +       E +      +E +    HE       +++           L   + V +G        V        KV+W+ NGQ          +  +    L +L  +PED  +Y+C AKN+ GEA SS   I 
Sbjct:   37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTS-ATLIVKDEKGLVVETQLPEGRKGAHRIDEMER-LAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVT--GYPAPKVNWYLNGQ----------LIRKNEHTLMLLEVFPEDAAIYSCSAKNDYGEATSSAPLIV 239

HSP 2 Score: 73.559 bits (179), Expect = 4.223e-14
Identity = 47/179 (26.26%), Postives = 84/179 (46.93%), Query Frame = 2 
Query:  800 KDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFE-NNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV 1330
            ++ +S++ +   G  H E  + P  D T+ V+W  +G+ ++  +R   V + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V ++ L E      +  E   +  E        E+Q +   P      + V + EG+ A+F   +  +P   + W +N + +
Sbjct:   24 EEYKSSVRMKCFGPAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTSATLI--VKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVTGYPAPKVNWYLNGQLI 200
[Close]
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000044036.1 (pep primary_assembly:ASM223467v1:21:968974:997146:1 gene:ENSORLG00000030355.1 transcript:ENSORLT00000044036.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.7886 bits (177), Expect = 2.400e-13
Identity = 47/179 (26.26%), Postives = 84/179 (46.93%), Query Frame = 2 
Query:  800 KDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEK-IVSKSNLNESSISQLKKFE-NNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEV 1330
            ++ +S++ +   G  H E  + P  D T+ V+W  +G+ ++  +R   V + GYC+LD    Y  D GV TC A N  G  ++S + I  + DEK +V ++ L E      +  E   +  E        E+Q +   P      + V + EG+ A+F   +  +P   + W +N + +
Sbjct:   24 EEYKSSVRMKCFGPAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTSATLI--VKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVTGYPAPKVNWYLNGQLI 200

HSP 2 Score: 65.0846 bits (157), Expect = 7.757e-11
Identity = 74/298 (24.83%), Postives = 111/298 (37.25%), Query Frame = 2 
Query:  449 PIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKD-----------LESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            P     ++ P GD ++ VEWL DGKPL    R   V E GY  LD   +   DSGV  C A +K G +Q++ AT+ V  E  +       E +      +E +    HE       +++           L   + V +G        V        KV+W+ NGQ +           + Y +L I  T  E +G Y C   N  G++  S               S+L    + QLK+ E           R  +    D+ P F K ++   + +G    F   +   P   I W
Sbjct:   37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTS-ATLIVKDEKGLVVETQLPEGRKGAHRIDEMER-LAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVT--GYPAPKVNWYLNGQLIR---------KKNY-SLVINKTQTEHEGEYFCSVSNPFGQSTCS---------------SHL----LVQLKEPERG---------REVDLTPHDKPPKFTKAIESSEVPQGGQCFFRYVVTGQPLPDIQW 292
[Close]
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000036245.1 (pep primary_assembly:ASM223467v1:17:27817163:27827046:1 gene:ENSORLG00000022942.1 transcript:ENSORLT00000036245.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 3.972e-12
Identity = 99/403 (24.57%), Postives = 160/403 (39.70%), Query Frame = 2 
Query:  395 APRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIE-EDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAV--HLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN--------------------DAFR--NTEDQEDDQAPS-FDKGL--QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINY-LELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHI 1516
            APRFL+      +V       LS  +   G  +  V W KD   L +G RF TV E G V    +Y +  EDSG Y+C AK+  G+  +   T++V   A++                          S ++P F     S+  V  GG V  H      P  +     +W K+G+ +   +R   V D     L I      D G YTC A+N+ G ++++ +      D +  S  + N S +S L+K +  ++++I+                    D     N E Q    A +   KG+  +  T+ EG  AK    +  HP+  I+W  +   +     +  Y D   N+ L++  C +         ATN  GQ  S  ++TV     + + L+ +
Sbjct:    9 APRFLTR-PKAFSVCVGKDAALSCTIV--GSPTPVVSWEKDKLKLTSGGRFKTV-EDGDVYRLTIYDLTLEDSGQYMCRAKNSVGEAYA-AVTLKVALPAEM--------------------------SQRAPAFMVK-PSSTRVGLGGDVVFHCRVAAHPEAN----FEWEKDGRYLGETNRIKIVSDGDSSTLKIQSVRSLDGGTYTCRAQNSAGRSQAAATLGVDTQDARHTS-VDKNTSLLSHLQKRKEEMKKDISIYHSEEKSSSVSSSSTTNVADVLSSLNLEHQLRASALAKLPKGVFTRTCTVTEGKHAKLSCLVTGHPKPHIIWRKDGTNISEGRRHVIYEDQAENFILKILYCKQSDNGLYTCNATNMAGQTYSAVLVTVKPKVPFRRKLQDV 374
[Close]
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000046039.1 (pep primary_assembly:ASM223467v1:21:27279628:27287449:1 gene:ENSORLG00000030310.1 transcript:ENSORLT00000046039.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.5438 bits (153), Expect = 6.898e-11
Identity = 50/197 (25.38%), Postives = 83/197 (42.13%), Query Frame = 2 
Query:  446 DPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCG 1036
            +P+RL  QV    D ++SV W +DGK +         FE    +L+I  S  +DSG YVC+A +++G   S+     VT + K+   ++ +                  E S SP F      N +V +               + + ++W K+G  +    R+   F      L+I   + +D G+Y C A+N  G
Sbjct:   38 NPLRLECQVDE--DTAVSVTWTRDGKKVHQSMDCKLSFEDKVAILEIPKSKLKDSGKYVCMATNEAG---SSTCEAVVTVQGKIFMIISLL------------------ELSSSPRFKVQSTDNSSVLK--------------IIKIDINWFKDGSEIVASDRYKVAFSNSVATLEICGAHAKDSGLYYCEARNEAG 197
[Close]
BLAST of Titin vs. Planmine SMEST
Match: SMESG000076529.1 (SMESG000076529.1)

HSP 1 Score: 1053.12 bits (2722), Expect = 0.000e+0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 2 
Query:  266 MNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTIXXXXXXXXXXXVAVKSQVEYDTNY 1834
            MNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTIPQGKPKMPPPPVAVKSQVEYDTNY
Sbjct:    1 MNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTIPQGKPKMPPPPVAVKSQVEYDTNY 523
[Close]
BLAST of Titin vs. Planmine SMEST
Match: SMESG000029197.1 (SMESG000029197.1)

HSP 1 Score: 317.005 bits (811), Expect = 2.055e-97
Identity = 175/464 (37.72%), Postives = 273/464 (58.84%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENE-----------PQYPDKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDA-FRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNIS 1741
            + P F+ + E     NE DP  L+++V PAGD +L+V+W KDG PL  G+RF+T FERG+ +LDILY+ +EDSG Y CVAK+  G +Q++P+T+ V     +D +V  + N H+ Q+  E           P+  D H  +  + P F K +     +  G +VHLET V P  D +L ++W KNG+ ++ G RFN VFDRG+  LDI+    ED G Y C A+N  G+A+S+  ++    +E +V+KS L + S+  L+  +    E+++ + FR  E++E++  P FD    ++T++EGN A+F++ +  +PR  I W +N   VI  S+ K Y DG+I+YLE  RC   G+  I V A N LG+  ++  L+V   N++  +LK   PENP++K+++LKKV V+PEL  A  K KP+A ++   ER A       ++ +  +AE LY  V + L+    +S
Sbjct:  201 DPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVN----LDESV--VLNSHLPQEMTESIAAIEASLNTPKRQDIHIDAPIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSNFRYEEEEENESLPHFDIKPINLTVEEGNPARFLVKVSGYPRPQIQWFLNECLVIPTSIQKVYSDGSISYLEFQRCKIPGESFIVVSAKNKLGEAVANATLSVIPENDFRPELKRTDPENPFRKLSTLKKVAVSPELQNAFRKTKPSAVQLSNYERGAP------RSKEASDAENLYANVQSRLRRSDKLS 652

HSP 2 Score: 94.3597 bits (233), Expect = 9.300e-20
Identity = 49/126 (38.89%), Postives = 68/126 (53.97%), Query Frame = 2 
Query:  773 ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNI 1150
            E +  P F K  E     N+G    L   V P  D  L V W+K+G  ++ GSRFN  F+RG+  LDILYT+ ED G Y+CVAKNN G  ++S S +    DE +V  S+L +     +   E ++
Sbjct:  198 EITDPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQEMTESIAAIEASL 323
[Close]
BLAST of Titin vs. Planmine SMEST
Match: SMESG000029197.1 (SMESG000029197.1)

HSP 1 Score: 310.457 bits (794), Expect = 7.362e-95
Identity = 175/470 (37.23%), Postives = 273/470 (58.09%), Query Frame = 2 
Query:  392 EAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENE-----------PQYPDKHESS--QSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDA-FRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAP------ENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNIS 1741
            + P F+ + E     NE DP  L+++V PAGD +L+V+W KDG PL  G+RF+T FERG+ +LDILY+ +EDSG Y CVAK+  G +Q++P+T+ V     +D +V  + N H+ Q+  E           P+  D H  +  + P F K +     +  G +VHLET V P  D +L ++W KNG+ ++ G RFN VFDRG+  LDI+    ED G Y C A+N  G+A+S+  ++    +E +V+KS L + S+  L+  +    E+++ + FR  E++E++  P FD    ++T++EGN A+F++ +  +PR  I W +N   VI  S+ K Y DG+I+YLE  RC   G+  I V A N LG+  ++  L+V   N++  +LK   P      ENP++K+++LKKV V+PEL  A  K KP+A ++   ER A       ++ +  +AE LY  V + L+    +S
Sbjct:  201 DPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNV----NLDESV--VLNSHLPQEMTESIAAIEASLNTPKRQDIHIDAPIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSNFRYEEEEENESLPHFDIKPINLTVEEGNPARFLVKVSGYPRPQIQWFLNECLVIPTSIQKVYSDGSISYLEFQRCKIPGESFIVVSAKNKLGEAVANATLSVIPENDFRPELKRTDPAILLRNENPFRKLSTLKKVAVSPELQNAFRKTKPSAVQLSNYERGAP------RSKEASDAENLYANVQSRLRRSDKLS 658

HSP 2 Score: 94.3597 bits (233), Expect = 9.984e-20
Identity = 49/126 (38.89%), Postives = 68/126 (53.97%), Query Frame = 2 
Query:  773 ESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNI 1150
            E +  P F K  E     N+G    L   V P  D  L V W+K+G  ++ GSRFN  F+RG+  LDILYT+ ED G Y+CVAKNN G  ++S S +    DE +V  S+L +     +   E ++
Sbjct:  198 EITDPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQEMTESIAAIEASL 323
[Close]
BLAST of Titin vs. Planmine SMEST
Match: SMESG000041478.1 (SMESG000041478.1)

HSP 1 Score: 305.834 bits (782), Expect = 4.302e-92
Identity = 176/458 (38.43%), Postives = 260/458 (56.77%), Query Frame = 2 
Query:  398 PRFLSNLE-NEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV--DNNVNFIENQHVQQDE-NEPQYPDKHES--SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTG 1747
            P F+  ++  EI+V+E  P      + P     LS +W  +G+ + T +R +   E     L+ILY++  D+G Y+CV  S+ G+ +S P T+ V  E  +  D     I   H  + E N P+   K +   +  P F K LE    +N+G +V L   V+P ND +L V+W+KN   +  GSR    +DRG   L+I+    ED G Y  +A N  G+ +S    I+C   EKIV+ S+L  + +   +LK  E ++       F+NTED +++  P+FD    ++T  EG  AKF++ +   P  ++ W I++  V+ DSV K Y DG+INYLE+ RC+E+G   + V ATNPLG    +  LTV  + ++  DL+HI PENP+KKMA LKKV+ T EL  A  K KPTAE+IVKIE  +E K++  K+PDV+E E+LYD+VAA L+   NI  G
Sbjct:  335 PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRK--NIGPG 790

HSP 2 Score: 265.003 bits (676), Expect = 5.154e-77
Identity = 161/436 (36.93%), Postives = 243/436 (55.73%), Query Frame = 2 
Query:  239 TTEIATNTNMNS--EDSNLPNGNIVNAQGNHVA------SFGRPKGHGSVQSQYESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP---------DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGL--QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEV-GQHNIRVVATNPLGQMASD-----CIL 1471
            + E   N+N NS  ++  +PNG  +      V       S   P+  GS         +++P F  +LE+E+ V+E +P+RL ++V PA D SL + W KDGK L  G+RFST+F+RG++VLDILY+IE+D+G YVCVAKS SG  QSN A VR+ PE+ VD      E    + +  +  Y          DKH +   P F   L +++T+ QG  +H+E  ++  +D +L  +W K+G S+ LGSR + ++DRGYCALDI + YPED+G Y+CVA+N+ G+AE+    +TC P+E I++KSNL ESSISQLK  EN  +E      R  +       PSF + +  +++++ EG  A F + I             N + +S   N +Y +G I    +  C+ + G+  + + A N L  + SD     C+L
Sbjct:    6 SMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR-RGS------DHLYDSPTFRQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEE----YARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDP----------GNGQKLS---NDWYHNGEI----VKTCSRLTGKLEMNISALNILYTLPSDTGSYICVL 413
[Close]
BLAST of Titin vs. Planmine SMEST
Match: SMESG000005752.1 (SMESG000005752.1)

HSP 1 Score: 69.707 bits (169), Expect = 8.538e-12
Identity = 75/283 (26.50%), Postives = 120/283 (42.40%), Query Frame = 2 
Query:  500 VEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVF-DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEI----ND--------AFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVW 1309
            ++W K+G  +     F+  ++   V L I     +DSG Y C+ K+ +G  + N ATV V           F+                      +P F   L+S   + +G  + L   V+     T ++ W+K+GQ ++    FN V  D    +L I    P D G Y   AKN  GEA +          E IV+++ ++E +I + +K + N+Q +I    ND         F  T+ +E  Q P F K L+ + I EG  A F +    +P   +VW
Sbjct: 4050 IKWYKNGAEINKSPEFTLSYKENRVSLTIPEVTPKDSGTYKCLVKNVAGDTE-NYATVTV--------KTIFM----------------------APKFIFGLQSQ-QILEGTNIKLSVKVE--GFPTPEIKWYKDGQVITSSPNFNLVVEDDNIFSLIIPNVRPMDQGKYCVSAKNKAGEAFTM--------GELIVAENKISEQTIVEQRK-KTNVQVDIIVPQNDNRLPQSSKMFSPTKKKEKIQPPEFVKILEPLKIKEGKKAFFSVEYKGNPEPVVVW 4289

HSP 2 Score: 69.3218 bits (168), Expect = 1.149e-11
Identity = 77/288 (26.74%), Postives = 127/288 (44.10%), Query Frame = 2 
Query:  401 RFLSNLENEIT--VNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTR--FSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQS-----------NPATVRVTPEAKVDNNV---------------NFIE-NQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVF--DRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN 1165
            +F S LE EIT  V E     L+ +     D+ +SV+W  D   L+   R   + + E     L I+   E+D+G Y+C+  S   Q ++           +  T+ VT  AK D  +                FIE +    + E E +Y    E +++P F  DL   +TV  G  + L  +V   + +  K  W+ N + +         +  + G C L+I   +PED G+Y CVA+N+ G AE   ++   I DE     ++ NE  + ++K  E +  ++IN
Sbjct: 6223 KFKSELEPEITKLVGE----TLNLECCYDSDSPVSVKWTLDDVELIESPRVNLALISEVQVCTLTIVNLNEDDTGEYMCIITSDESQAETGTFVTIVQPGEDEQTITVTSSAKSDIEIIKPDLTSIDDSEVSFTFIEKDTKDTKIEKEQEYEIAVELAEAPNFISDLYP-VTVMDGEKLCLSCVVK-ASPMPQKTIWYHNNEIIEPNDELKLTYSTEDGKCLLEIAEVFPEDAGLYKCVARNDYGVAE---TETEVIVDEIEYVSNDENEEIVIEIKPTEESSVKQIN 6501

HSP 3 Score: 63.929 bits (154), Expect = 5.347e-10
Identity = 62/254 (24.41%), Postives = 113/254 (44.49%), Query Frame = 2 
Query:  452 IRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKS--KSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIV--DPRNDVTLKVDWHKNGQSMSLGSRFNAVFDR--GYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQE 1195
            + L  ++ PA D  + VEW K+  P+++    +  ++ G  +L I    EEDSG Y+C A S  +S    SN      + E +   +     +  +  D  +       E +      + L + +++ + G + L+ ++   P N VT    W+ +G  +   S    + ++  G C L +   +PED+G Y C  +N     ++  + +  IPD   V K  + ES + + K  E N    IND   N +D++
Sbjct: 9461 LLLECEITPAVD--VVVEWFKNLTPVISNEHIAITYDLGKCILMIAEVEEEDSGHYICKASSRERSISTSSNVTVKNDSSEMRESEDETIDASFEIDDDNKQSTDNVNSEFALRSVIKELLPNEMSIPESGKLLLQCVIVQSPENRVT----WYLDGDLLE-NSGIYEINNKPDGVCQLIVYEAFPEDEGYYCCKIENQFSTIQTG-TNVHIIPDSVAVQKPKI-ESKLPE-KAVEEN--SSINDFLLNMDDEQ 9702

HSP 4 Score: 62.3882 bits (150), Expect = 1.516e-9
Identity = 86/378 (22.75%), Postives = 146/378 (38.62%), Query Frame = 2 
Query:  377 ESKEFEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNP-ATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPDKHESSQSPYFSKDLES-NITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRG-YCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQ-DVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYA 1498
            E+K    P+F+  LE  I V E D ++L ++V   G  +  VEW K+G  +     +    +   V L I  +  +D+G Y  +A++  G  +S+   TV +TPE+                 +N PQ      S Q P   ++ +S  + +N+      + I  P  +V+    W+ N   +      + ++    Y  L I      D G YT  A N  GEA+                              F  ++  E    F  TE      AP F + L+  +T  EG   K ++++  +P   + W  N   V +    + + DG ++ L +    +       V+A NP G +  +  L V   N+Y 
Sbjct: 5041 ETKSTYPPKFVRELEKSIQVKEGDSVKLLAEVD--GYPTPVVEWYKNGIVIEKNPDYKLFRDGNVVSLYIHETFTDDTGEYNLIARNPFGSVKSSAHLTVTITPESA----------------DNVPQ------SKQIPRIIQEPKSVKLNLNKKAVFEAKVIGSPVPEVS----WYHNDIFIEESENVSIIYSNNEYTKLIINSVKETDAGTYTVKAVNIVGEAQ------------------------------FSTSLTVEAPTKFPKTE------APKFIRSLEPSMTAKEGEPCKLLVDVMGNPNPKLEWYKNGVLVDNMPDYQLFRDGNVHTLYIKELFQDDSGKYIVIANNPNGTVQCETNLVVT--NDYT 5352

HSP 5 Score: 54.299 bits (129), Expect = 4.561e-7
Identity = 61/238 (25.63%), Postives = 103/238 (43.28%), Query Frame = 2 
Query:  389 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEE-DSGVYVCVAKSKSGQ-----------NQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYPD------KHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAES 1048
            F AP+F+  L+++  + E   I+LS +V   G  +  ++W KDG+ + +   F+ V E   +   I+ ++   D G Y   AK+K+G+           N+ +  T+ V    K +  V+ I  Q+   D   PQ         K E  Q P F K LE  + + +G        V+ + +    V W + G  +  G  +      G+  L I   + +D G +TC  +N  G  ES
Sbjct: 4112 FMAPKFIFGLQSQ-QILEGTNIKLSVKVE--GFPTPEIKWYKDGQVITSSPNFNLVVEDDNIFSLIIPNVRPMDQGKYCVSAKNKAGEAFTMGELIVAENKISEQTI-VEQRKKTNVQVDIIVPQN---DNRLPQSSKMFSPTKKKEKIQPPEFVKILEP-LKIKEGKKAFFS--VEYKGNPEPVVVWLREGLQLENGPDYQITTKPGFSQLTIAEVFKDDAGKFTCRLENKAGRIES 4339

HSP 6 Score: 51.6026 bits (122), Expect = 2.562e-6
Identity = 34/106 (32.08%), Postives = 54/106 (50.94%), Query Frame = 2 
Query:  764 DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESS-HSQITCIPD 1078
            D+ +S+  P F ++LE +I V +G +V L   VD     T  V+W+KNG  +     +    D    +L I  T+ +D G Y  +A+N  G  +SS H  +T  P+
Sbjct: 5040 DETKSTYPPKFVRELEKSIQVKEGDSVKLLAEVDGYP--TPVVEWYKNGIVIEKNPDYKLFRDGNVVSLYIHETFTDDTGEYNLIARNPFGSVKSSAHLTVTITPE 5143
The following BLAST results are available for this feature:
BLAST of Titin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TTN2.233e-2324.15titin [Source:HGNC Symbol;Acc:HGNC:12403][more]
TTN2.271e-2324.15titin [Source:HGNC Symbol;Acc:HGNC:12403][more]
TTN2.291e-2324.15titin [Source:HGNC Symbol;Acc:HGNC:12403][more]
TTN2.775e-2324.15titin [Source:HGNC Symbol;Acc:HGNC:12403][more]
TTN2.898e-2324.15titin [Source:HGNC Symbol;Acc:HGNC:12403][more]
back to top
BLAST of Titin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ketn-11.612e-4429.77KETtiN (Drosophila actin-binding) homolog; Kettin ... [more]
ketn-11.648e-4429.77KETtiN (Drosophila actin-binding) homolog; Kettin ... [more]
ketn-11.737e-4429.77KETtiN (Drosophila actin-binding) homolog; Kettin ... [more]
ketn-11.739e-4429.77KETtiN (Drosophila actin-binding) homolog; Kettin ... [more]
ketn-11.747e-4429.77KETtiN (Drosophila actin-binding) homolog; Kettin ... [more]
back to top
BLAST of Titin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sls3.621e-4529.50gene:FBgn0086906 transcript:FBtr0332683[more]
sls4.170e-4529.50gene:FBgn0086906 transcript:FBtr0072848[more]
sls4.648e-4529.50gene:FBgn0086906 transcript:FBtr0309042[more]
sls4.830e-4529.50gene:FBgn0086906 transcript:FBtr0332677[more]
sls6.625e-4529.50gene:FBgn0086906 transcript:FBtr0332679[more]
back to top
BLAST of Titin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttn.21.854e-2425.12titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GEN... [more]
ttn.25.613e-2425.00titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GEN... [more]
ttn.19.538e-2126.23titin, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GEN... [more]
obsl1b7.009e-1124.18obscurin like cytoskeletal adaptor 1b [Source:ZFIN... [more]
obsl1b7.262e-1124.18obscurin like cytoskeletal adaptor 1b [Source:ZFIN... [more]
back to top
BLAST of Titin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PALLD4.024e-924.83palladin, cytoskeletal associated protein [Source:... [more]
PALLD1.952e-825.27palladin, cytoskeletal associated protein [Source:... [more]
PALLD2.052e-825.27palladin, cytoskeletal associated protein [Source:... [more]
PALLD2.193e-825.27palladin, cytoskeletal associated protein [Source:... [more]
PALLD2.200e-825.27palladin, cytoskeletal associated protein [Source:... [more]
back to top
BLAST of Titin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ttn1.454e-2224.68titin [Source:MGI Symbol;Acc:MGI:98864][more]
Ttn1.923e-2224.89titin [Source:MGI Symbol;Acc:MGI:98864][more]
Ttn2.048e-2224.89titin [Source:MGI Symbol;Acc:MGI:98864][more]
Ttn2.048e-2224.89titin [Source:MGI Symbol;Acc:MGI:98864][more]
Ttn2.475e-2224.89titin [Source:MGI Symbol;Acc:MGI:98864][more]
back to top
BLAST of Titin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9I7U4|TITIN_DROME8.429e-4429.50Titin OS=Drosophila melanogaster OX=7227 GN=sls PE... [more]
sp|Q8WZ42|TITIN_HUMAN1.392e-2224.15Titin OS=Homo sapiens OX=9606 GN=TTN PE=1 SV=4[more]
sp|A2ASS6|TITIN_MOUSE1.776e-2124.89Titin OS=Mus musculus OX=10090 GN=Ttn PE=1 SV=1[more]
sp|O01761|UNC89_CAEEL4.694e-1525.31Muscle M-line assembly protein unc-89 OS=Caenorhab... [more]
sp|P11799|MYLK_CHICK5.810e-1324.66Myosin light chain kinase, smooth muscle OS=Gallus... [more]
back to top
BLAST of Titin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504Y5201.043e-10338.48Titin OS=Fasciola gigantica OX=46835 GN=FGIG_04181... [more]
A0A4E0RJF19.699e-10338.04Titin OS=Fasciola hepatica OX=6192 GN=D915_002543 ... [more]
A0A183A5N11.126e-10038.48Uncharacterized protein OS=Echinostoma caproni OX=... [more]
H2KSJ11.213e-9838.78Titin OS=Clonorchis sinensis OX=79923 GN=CLF_10948... [more]
A0A3R7CUY51.492e-9838.78Titin OS=Clonorchis sinensis OX=79923 GN=sls PE=4 ... [more]
back to top
BLAST of Titin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000044348.11.155e-2027.95pep primary_assembly:Astyanax_mexicanus-2.0:19:232... [more]
obsl1b2.776e-1324.87obscurin like cytoskeletal adaptor 1b [Source:ZFIN... [more]
OBSCN5.205e-1324.59obscurin, cytoskeletal calmodulin and titin-intera... [more]
OBSCN7.832e-1324.34obscurin, cytoskeletal calmodulin and titin-intera... [more]
obsl1a8.024e-1227.94obscurin like cytoskeletal adaptor 1a [Source:ZFIN... [more]
back to top
BLAST of Titin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSPMAT00000000826.14.519e-2624.40pep scaffold:Pmarinus_7.0:GL476876:251045:270583:1... [more]
ENSPMAT00000002502.12.972e-2525.85pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-... [more]
mylka1.456e-1027.67myosin, light chain kinase a [Source:ZFIN;Acc:ZDB-... [more]
ENSPMAT00000004663.16.994e-926.29pep scaffold:Pmarinus_7.0:GL479759:1154:24713:-1 g... [more]
back to top
BLAST of Titin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Titin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO495533.276e-1524.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495523.631e-1122.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495581.578e-1028.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495503.268e-1027.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495492.046e-921.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Titin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000027090.14.015e-1826.61pep primary_assembly:ASM223467v1:21:27247530:27253... [more]
ENSORLT00000046443.14.251e-1529.03pep primary_assembly:ASM223467v1:21:968974:990901:... [more]
ENSORLT00000044036.12.400e-1326.26pep primary_assembly:ASM223467v1:21:968974:997146:... [more]
ENSORLT00000036245.13.972e-1224.57pep primary_assembly:ASM223467v1:17:27817163:27827... [more]
ENSORLT00000046039.16.898e-1125.38pep primary_assembly:ASM223467v1:21:27279628:27287... [more]
back to top
BLAST of Titin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076529.10.000e+0100.00SMESG000076529.1[more]
SMESG000029197.12.055e-9737.72SMESG000029197.1[more]
SMESG000029197.17.362e-9537.23SMESG000029197.1[more]
SMESG000041478.14.302e-9238.43SMESG000041478.1[more]
SMESG000005752.18.538e-1226.50SMESG000005752.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032833 ID=SMED30032833|Name=Titin|organism=Schmidtea mediterranea sexual|type=transcript|length=2208bp
CAGAAAATCACAGACTCTTCGAGCATGTCTAAGAATGACATAGAAATTAA
AATTGTGCCGAACGTTCTGTTTATCGATTTATGTGCATTTTTAATTTCCC
AATTTCGGTACCTGCAGAGCACGCATACTTGTGTTATTCTAAAGAAAAAA
GACCAACACAAAGTCGAGTGTGTTAGACAAAATCTTTTAAGCCCAAATCA
CTGTTTTAAATATTTAGAAATAAAATCTTTCATTTTCAACAACTGAAATA
GCCACTAATACTAATATGAACTCTGAAGATTCGAACCTCCCAAACGGAAA
TATTGTCAACGCTCAGGGAAACCACGTAGCTTCATTTGGTAGACCTAAAG
GACATGGCAGCGTTCAAAGTCAATATGAATCGAAAGAGTTTGAAGCACCA
CGGTTTTTATCGAATTTAGAAAATGAAATCACTGTCAATGAAGAAGACCC
AATAAGACTTTCAAGTCAAGTTGCTCCAGCGGGAGATAATTCTCTTTCTG
TTGAATGGTTAAAAGATGGAAAACCCCTTTTAACAGGAACAAGATTTAGT
ACTGTTTTCGAAAGAGGATATGTGGTGCTCGATATTCTATATTCAATTGA
AGAGGATAGTGGCGTTTATGTTTGTGTAGCTAAAAGCAAATCTGGGCAAA
ATCAAAGTAACCCTGCTACGGTTCGGGTGACACCAGAAGCTAAAGTGGAT
AATAATGTAAATTTCATCGAAAATCAACACGTTCAACAAGATGAAAATGA
GCCTCAATATCCTGACAAACATGAATCATCGCAGTCCCCATATTTTTCGA
AAGATCTTGAGTCTAATATTACTGTTAATCAAGGAGGTGCGGTGCATTTG
GAAACTATTGTTGATCCACGAAACGATGTAACGTTAAAAGTTGATTGGCA
TAAAAACGGACAATCAATGTCATTGGGAAGCCGGTTCAATGCGGTATTTG
ATCGAGGATATTGTGCCCTCGATATTTTGTATACATATCCTGAAGATGAT
GGAGTTTACACATGTGTTGCTAAAAATAATTGCGGAGAAGCTGAAAGTAG
TCATTCACAAATCACTTGTATTCCTGATGAAAAAATAGTTTCAAAGTCAA
ATTTGAACGAATCTTCGATTTCTCAACTGAAAAAATTTGAAAACAACATC
CAAGAAGAAATTAACGATGCGTTCAGAAATACGGAAGACCAAGAAGACGA
TCAAGCGCCAAGTTTTGACAAAGGTCTTCAAGATGTAACAATAGATGAAG
GAAATGCAGCGAAGTTCATAATTAATATTCATAGTCATCCTAGGGCTTCC
ATCGTTTGGTCAATTAACAATGAAGAAGTTATCTCGGATTCGGTTAACAA
ATTTTATGGAGACGGGACCATTAACTATTTGGAGCTTACACGATGCACGG
AAGTTGGTCAGCACAACATTCGTGTGGTAGCCACCAATCCATTGGGTCAG
ATGGCGTCGGACTGTATCTTAACAGTAAATTCAATTAACAACTATGCACA
GGATTTAAAGCACATTGCTCCAGAAAATCCATACAAAAAAATGGCTTCAT
TGAAAAAAGTAGATGTTACACCCGAACTTACAACTGCATTACATAAACCA
AAACCGACTGCAGAGAAAATAGTTAAAATAGAAAGAAATGCTGAGGTCAA
GGCTCGTCACACGAAAACGCCAGATGTTTTGGAAGCGGAACAATTGTACG
ATAAAGTGGCGGCAAACTTAAAAAGTAAAGGAAATATTTCAACAGGAAAG
CGACAAACGATTCCACAAGGTAAACCAAAAATGCCTCCACCACCGGTGGC
TGTGAAATCGCAGGTCGAGTATGATACCAATTACCCTAGTGATGATATGC
CACCACCGCCGTCAGTCTAAATTAATTTCATGAATGCTACTTTCAAACAT
TTTTAAGAACGCTTTTCCTATTCAATTTGATTTTGAATGTGAAATTTATC
AAACATTTTATATTTGAAAACGTTATGTTTTAATCCCTTTGCTTGATAAT
AAGGTTTAATAAGCTATAATAAATCTCTAGCTCTTATTCACATGTATTGT
CTTGCCTAAATATAATCTTGGTTTCTAAACCCCTAAATCCACCATTTTAG
GATTTAAGTTTGGCACATTTTTCGGAAATGTAGGATAATCTCATTTTCCC
AAGATTTTCGTTTAGGAAATTTGGAATATTTTTGACAATTTATTAAAACA
TTTTCTAG
back to top

protein sequence of SMED30032833-orf-1

>SMED30032833-orf-1 ID=SMED30032833-orf-1|Name=SMED30032833-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=535bp
MNSEDSNLPNGNIVNAQGNHVASFGRPKGHGSVQSQYESKEFEAPRFLSN
LENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFER
GYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNF
IENQHVQQDENEPQYPDKHESSQSPYFSKDLESNITVNQGGAVHLETIVD
PRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTC
VAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEIN
DAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSI
NNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDC
ILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAE
KIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLKSKGNISTGKRQTIP
QGKPKMPPPPVAVKSQVEYDTNYPSDDMPPPPSV*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR003599Ig_sub
IPR013783Ig-like_fold
IPR013098Ig_I-set
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003779actin binding
GO:0008307structural constituent of muscle
Vocabulary: cellular component
TermDefinition
GO:0000794condensed nuclear chromosome
GO:0005634nucleus
GO:0005694chromosome
GO:0005737cytoplasm
GO:0005863striated muscle myosin thick filament
GO:0005875microtubule associated complex
GO:0030017sarcomere
GO:0030018Z disc
GO:0031674I band
Vocabulary: biological process
TermDefinition
GO:0007049cell cycle
GO:0007062sister chromatid cohesion
GO:0007067mitotic nuclear division
GO:0007076mitotic chromosome condensation
GO:0007275multicellular organism development
GO:0007498mesoderm development
GO:0007517muscle organ development
GO:0007519skeletal muscle tissue development
GO:0007520myoblast fusion
GO:0007522visceral muscle development
GO:0007525somatic muscle development
GO:0016203muscle attachment
GO:0019233sensory perception of pain
GO:0035206regulation of hemocyte proliferation
GO:0040011locomotion
GO:0045214sarcomere organization
GO:0051301cell division
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 281..301
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 488..534
NoneNo IPR availablePANTHERPTHR13817:SF113coord: 33..282
NoneNo IPR availablePANTHERPTHR13817TITINcoord: 33..282
NoneNo IPR availableCDDcd00096Igcoord: 73..131
e-value: 2.00727E-8
score: 49.1628
NoneNo IPR availableCDDcd00096Igcoord: 206..262
e-value: 6.01159E-8
score: 47.622
IPR003599Immunoglobulin subtypeSMARTSM00409IG_3ccoord: 52..139
e-value: 1.8E-5
score: 34.2
coord: 182..269
e-value: 2.6
score: 16.5
coord: 321..405
e-value: 180.0
score: 0.6
IPR013098Immunoglobulin I-setPFAMPF07679I-setcoord: 46..131
e-value: 5.4E-10
score: 39.2
coord: 175..262
e-value: 2.5E-11
score: 43.5
coord: 315..404
e-value: 4.8E-8
score: 32.9
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 169..268
e-value: 5.7E-15
score: 57.3
coord: 40..141
e-value: 1.1E-15
score: 59.6
coord: 309..407
e-value: 1.3E-11
score: 46.6
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 175..267
score: 9.7
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 45..137
score: 9.736
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 41..138
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 313..406
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 174..262