SCO-spondin

Overview
NameSCO-spondin
Smed IDSMED30032818
Length (bp)2338
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SCO-spondin (SMED30032818) t-SNE clustered cells

Violin plots show distribution of expression levels for SCO-spondin (SMED30032818) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SCO-spondin (SMED30032818) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SCO-spondin (SMED30032818) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032818

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 41

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_074463SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_019902SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_025596SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_015921SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_015920SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 SmedASXL_015919SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30032818h1SMcG0018856 dd_Smed_v4_1647_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30032818h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032818h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30032818h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032818h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig46457uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig46457newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig45454uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig45454newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018854 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018854 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018855 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018855 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018853 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018853 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018855 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018855 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032818h1SMcG0018856 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30032818h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30032818h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30032818h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 dd_Smed_v6_651_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 dd_Smed_v6_1647_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018855 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032818h1SMcG0018856 dd_Smed_v6_12625_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30032818h1SMcG0018856 OX_Smed_1.0.05555ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30032818h1SMcG0018856 Contig46551uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032818h1SMcG0018856 Contig46551newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30032818h1SMcG0018856 dd_Smed_v4_1647_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30032818h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
gutSMED30032818h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SCO-spondin vs. TrEMBL
Match: A0A1I8G0U9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 6.837e-23
Identity = 169/634 (26.66%), Postives = 266/634 (41.96%), Query Frame = 3
Query:  147 SRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTL--TVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCEC---LPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKD-CVSRKECMCLDENGGIRKNGEEWTINCKNYVC-DGIVTKRIFNDCQGVSLNCPP-EFQIMVDCCMVCNIXXXXXXXPHIS----------VCKF----ENQTLQ--VGETVIR-KVDFCEDDTCVCNQVGGLTCTR---NGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCD--IGDLENLELNQFKKYCNLTNLICG---ECPSTDFRYIDSAT--GIQYCLG-----ACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQT--CRKQICLKFQENCLKDGLCDGSICECKEP-LVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICS 1919
            S G     F+ + TD  G     ++V+     G  +  +V  L++  C+E      ++      TT +C      +I+ C+ + C +G W  +K C + C      +  + + CC+C      +T  + TTT    TTTP C  P+  + C++   +      + S+  +  C+ GC+CP     N  + CV  ++C C   +G +R  G+ W ++C  Y C +  ++      C+  S+ CP     +  DCC  C     T                 VC +     N+T++  + ET +   V       C C     + C     +  +   C ESC  K      V  N    C C C  S +            C+LPC+  +G L+   L    K    T   C      P      + SAT  G ++C         C +NGK++     V ++ + G+ IPC  C C  +    C    NC  TTT+   TT+    +  K   EESV  T  CR++IC   + +C+  G C+   CECK   L FNGT C+N   C C D     + K GE W + TD C+ H+C  N+I+T + R +CP   CP   ++ +     KCC IC+
Sbjct: 1953 SSGLVSLSFSNETTD--GVSATAVRVTFPRGSGVKVNPSVSGLLISACFEV-----STTVVAPTTTPICASGDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRKCHYIEPECSSEGKFPCESGCQCPSGYRMNSTNHCVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTISNLGRVPCR--SVTCPAGSMMVTADCCPSCVTKPPTTGPETTLPPTTTVAPKRVCHYLDPATNRTVEKSINETWVETAVGTSCKRVCSCTTAAMVECKSVAISAAEKAQCLESCQKKYPTDKDVKANFDSSCRCGCTSSCTELKV--------CELPCNETVGSLDEQNLYCLSKGLFKTEPKCTCSEALPGYGSSEVRSATFGGAKHCFAQKTCTKSCNYNGKRVPIHGVVKEVDSTGSVIPCRECTCGESFTMSCNTVKNCTVTTTEAPKTTTAPGCSCAKP-YEESVNSTGKCREKICHYLKGSCVSRGECN---CECKGAYLFFNGTTCVNQANCYCKDENGALR-KNGEKWQNPTDPCKTHMCVDNQIQTSDRRPDCPKFVCPPDSVRQV--PPGKCCAICT 2562          
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267DLR6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026350g2 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 8.945e-23
Identity = 176/683 (25.77%), Postives = 281/683 (41.14%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKF-EFNFDQTDKNGEQFEYMKVSIRTNVGYTL--TVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCEC---LPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKD-CVSRKECMCLDENGGIRKNGEEWTINCKNYVC-DGIVTKRIFNDCQGVSLNCPP-EFQIMVDCCMVC----------NIXXXXXXXPHISVCKF----ENQTLQ--VGETVIR-KVDFCEDDTCVCNQVGGLTCTR---NGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCD--IGDLENLELNQFKKYCNLTNLICG---ECPSTDFRYIDSAT--GIQYCLG-----ACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQT--CRKQICLKFQENCLKDGLCDGSICECKEP-LVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICS 1919
            E   V  +TP+  +   I  LE+       V + F   SD     +  +  S      +F     +G     ++V+     G  +  +V  L++  C+E      ++      TT +C      +I+ C+ + C +G W  +K C + C      +  + + CC+C      +T  + TTT    TTTP C  P+  + C++   +      + S+  +  C+ GC+CP     N  + CV  ++C C   +G +R  G+ W ++C  Y C +  ++      C+  S+ CP     +  DCC  C               T T     VC +     N+T++  + ET +   V       C C     + C     +  +   C E C  K      V  N    C C C  S +            C+LPC+  +G L+   L    K    T   C      P      + SAT  G ++C         C +NGK++     V ++ + G+ IPC  C C  +    C    NC  TTT+   TT+    +  K   EESV  T  CR++IC   + +C+  G C+   CECK   L FNGT C+N   C C D     + K GE W + TD C+ H+C  N+I+T + R +CP   CP   ++ +     KCC +C+
Sbjct: 1921 EPRLVFAITPRPGSRTIITKLELTATRARSVEVEFTSGSDIWYSKRVATPSSSGLVSLSFSNETTDGVSATAVRVTFPRGSGVKVNPSVSGLLISACFE-----VSTTVVAPTTTPICASGDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRKCHYIEPECSSEGKFPCESGCQCPSGYRMNSTNHCVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTISNLGRVPCK--SVTCPAGSMMVTADCCPSCVTKPPTTGPETTLPPTTTVAPKRVCHYLDPATNRTVEKSINETWVETAVGTSCKRVCSCTTAAMVECKSVAISAAEKAQCLEICQKKYPTDKDVKANFDSSCRCGCTSSCTELKV--------CELPCNETVGSLDEQNLYCLSKGLFKTEPKCTCSEALPGYGSSEVRSATFGGAKHCFAQKTCTKSCNYNGKRVPIHGVVKEVDSTGSVIPCRECTCGESFTMSCNTVKNCTVTTTEAPKTTTAPGCSCAKP-YEESVNSTGKCREKICHYLKGSCVSRGECN---CECKGAYLFFNGTTCVNQANCYCKDENGALR-KNGEKWQNPTDPCKTHMCVDNQIQTSDRRPDCPKFVCPPDSVRQV--PPGKCCAVCT 2581          
BLAST of SCO-spondin vs. TrEMBL
Match: A0A267GUZ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026350g1 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 4.124e-22
Identity = 179/688 (26.02%), Postives = 283/688 (41.13%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKF-EFNFDQTDKNGEQFEYMKVSIRTNVGYTL--TVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCEC---LPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKD-CVSRKECMCLDENGGIRKNGEEWTINCKNYVC-DGIVTKRIFNDCQGVSLNCPP-EFQIMVDCCMVCNIXXXXXXXPHIS----------VCKF----ENQTLQ--VGETVIR-KVDFCEDDTCVCNQVGGLTCTR---NGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCD--IGDLENLELNQFKKYCNLTNLI-------CGEC-PSTDFRYIDSAT--GIQYCLG-----ACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQT--CRKQICLKFQENCLKDGLCDGSICECKEP-LVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICS 1919
            E   V  +TP+  +   I  LE+       V + F   SD     +  +  S      +F     +G     ++V+     G  +  +V  L++  C+E      ++      TT +C      +I+ C+ + C +G W  +K C + C      +  + + CC+C      +T  + TTT    TTTP C  P+  + C++   +      + S+  +  C+ GC+CP     N  + CV  ++C C   +G +R  G+ W ++C  Y C +  ++      C+  S+ CP     +  DCC  C     T                 VC +     N+T++  + ET +   V       C C     + C     +  +   C ESC  K      V  N    C C C  S +            C+LPC+  +G L+   L     YC    L        C E  P      + SAT  G ++C         C +NGK++     V ++ + G+ IPC  C C  +    C    NC  TTT+   TT+    +  K   EESV  T  CR++IC   + +C+  G C+   CECK   L FNGT C+N   C C D     + K GE W + TD C+ H+C  N+I+T + R +CP   CP   ++ +     KCC IC+
Sbjct: 1921 EPRLVFAITPRPGSRTIITKLELTATRARSVEVEFTSGSDIWYSKRVATPSSSGLVSLSFSNETTDGVSATAVRVTFPRGSGVKVNPSVSGLLISACFEV-----STTVVAPTTTPICASGDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRKCHYIEPECSSEGKFPCESGCQCPSGYRMNSTNHCVKSEDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTISNLGRVPCR--SVTCPAGSMMVTADCCPSCVTKPPTTGPETTLPPTTTVAPKRVCHYLDPATNRTVEKSINETWVETAVGTSCKRVCSCTTAAMVECKSVAISAAEKAQCLESCQKKYPTDKDVKANFDSSCRCGCTSSCTELKV--------CELPCNETVGSLDEQNL-----YCLSKGLFKTEPKCTCSEALPGYGSSEVRSATFGGAKHCFAQKTCTKSCNYNGKRVPIHGVVKEVDSTGSVIPCRECTCGESFTMSCNTVKNCTVTTTEAPKTTTAPGCSCAKP-YEESVNSTGKCREKICHYLKGSCVSRGECN---CECKGAYLFFNGTTCVNQANCYCKDENGALR-KNGEKWQNPTDPCKTHMCVDNQIQTSDRRPDCPKFVCPPDSVRQV--PPGKCCAICT 2581          
BLAST of SCO-spondin vs. TrEMBL
Match: A0A1I8GY38 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.916e-17
Identity = 154/580 (26.55%), Postives = 241/580 (41.55%), Query Frame = 3
Query:  270 IVKVCYESVSTAQTSAPTQEVTTSVCKEDSTD--IITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCEC---LPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVC-DGIVTKRIFNDCQGVSLNCPP-EFQIMVDCCMVC----------NIXXXXXXXPHISVCKF----ENQTLQ--VGETVIR-KVDFCEDDTCVCNQVGGLTCTR---NGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCD--IGDLENLELNQFKKYCNLTNLICG---ECPSTDFRYIDSAT--GIQYCLG-----ACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQT--CRKQICLKFQENCLKDGLCDGSICECKEP-LVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTI 1883
            +V + + + +T   SA    VT    +  S D  +I+ C+ + C +G W  +K C + C      +  + + CC+C      +T  + TTT    TTTP C  P+  + C++   +      + S+  +  C+ GC               +K+C C   +G +R  G+ W ++C  Y C +  ++      C+  S+ CP     +  DCC  C               T T     VC +     N+T++  + ET +   V       C C     + C     +  +   C ESC  K      V  N    C C C  S +            C+LPC+  +G L+   L    K    T   C      P      + SAT  G ++C         C +NGK++     V ++ + G+ IPC  C C  +    C    NC  TTT+   TT+    +  K   EESV  T  CR++IC   + +C+  G C+   CECK   L FNGT C+N   C C D     + K GE W + TD C+ H+C  N+I+T + R +CP   CP   ++ +
Sbjct: 1963 LVSLSFSNETTDGVSATAVRVTFPAAQASSGDKKVISECISQVCESGAWIRKKSCSIECGSSCSIVHFSGDACCKCSCGTTTTTRVVTTTTAPPTTTTPMCAAPMEPSDCMRKCRKCHYIEPECSSEGKFPCESGCH--------------QKDCSCRSPDGKVRSAGDVWVVDCIRYTCANNTISNLGRVPCR--SVTCPAGSMMVTADCCPSCVTKPPTTGPETTLPPTTTVAPKRVCHYLDPATNRTVEKSINETWVETAVGTSCKRVCSCTTAAMVECKSVAISAAEKAQCLESCQKKYPTDKDVKANFDSSCRCGCTSSCTELKV--------CELPCNETVGSLDEQNLYCLSKGLFKTEPKCTCSEALPGYGSSEVRSATFGGAKHCFAQKTCTKSCNYNGKRVPIHGVVKEVDSTGSVIPCRECTCGESFTMSCNTVKNCTVTTTEAPKTTTAPGCSCAKP-YEESVNSTGKCREKICHYLKGSCVSRGECN---CECKGAYLFFNGTTCVNQANCYCKDENGALR-KNGEKWQNPTDPCKTHMCVDNQIQTSDRRPDCPKFVCPPDSVRQV 2513          
BLAST of SCO-spondin vs. TrEMBL
Match: A0A0L8FZQ8 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22003392mg PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.223e-7
Identity = 55/169 (32.54%), Postives = 79/169 (46.75%), Query Frame = 3
Query:  345 CKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXX----------------XXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQE---CKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVC 794
            C E+ T I+  C  + CI+G +     C   C++    LV  D +CC+C+ ++T                    T+     TTT  C+ PL  T C+K    + C   +  NCT      C+ GC+CP NL FNG  CV    C+CLD+ G +RK G+ W  +C  Y C
Sbjct:  999 CIENETRILNECFNEICIDGEFQRVPACNKKCADN-ETLVIRDKECCQCVALTTPVTRTNVYTTTRYSSGTQWTTSISPTQTTTGVCEEPLERTDCMKEC--TKCHLLE-PNCTPTGPFICETGCDCPVNLVFNGTHCVYPGSCICLDDEGNLRKPGDVWIKDCYEYTC 1163          
BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000981.1 (pep scaffold:Pmarinus_7.0:GL476384:615201:646381:-1 gene:ENSPMAG00000000884.1 transcript:ENSPMAT00000000981.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 4.284e-6
Identity = 25/73 (34.25%), Postives = 31/73 (42.47%), Query Frame = 3
Query:  549 PFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEW 767
            PF   P  H     P C + C    L     Q+C  GC C +    +G  CV  ++C CLD NG     GE W
Sbjct:  771 PFPCEPGSHYESCAPPCPATCSNASLPGGCNQQCAEGCVCDDGFVLSGDKCVPTEQCGCLDNNGHYHPLGESW 843          
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 1318.52 bits (3411), Expect = 0.000e+0
Identity = 738/739 (99.86%), Postives = 739/739 (100.00%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIXXXXXXXPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 2219
            EISFVIKLTPKSNT+VNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE
Sbjct:  344 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 1082          

HSP 2 Score: 101.293 bits (251), Expect = 1.859e-21
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            EISFVIKLTPKSNTEVNIVNLEINVNINSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  177 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 272          

HSP 3 Score: 72.0182 bits (175), Expect = 1.600e-12
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVT 2207
            ICT++FCSEYTC+ EC+  E R  +C+ L+C  R KE F+N+T I CTR  +ENC STS  +  +T
Sbjct: 6667 ICTYMFCSEYTCKGECVTGESRNVDCSKLICVGRSKEGFRNDTYIVCTRRIEENCVSTSTSSPVLT 6732          

HSP 4 Score: 68.9366 bits (167), Expect = 1.450e-11
Identity = 33/56 (58.93%), Postives = 42/56 (75.00%), Query Frame = 3
Query:  123 EIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            +I+    K  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:   50 QILSPVRKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 105          

HSP 5 Score: 64.6994 bits (156), Expect = 2.672e-10
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTS 2198
            ICT+++CSEYTC+ EC+  E R  +C+ LVC    +EA+KN+T I CT+  +ENC ST+ +++
Sbjct: 6506 ICTYMYCSEYTCKGECVTGESRNVDCSKLVCVGLSEEAYKNDTYIVCTKRIEENCISTTTRST 6568          

HSP 6 Score: 51.9878 bits (123), Expect = 2.639e-6
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKST 2183
            IC + +CS YTC   CLN E R ++C N +C + L E FKN+T I C    + NC ST
Sbjct: 1376 ICIYGYCSVYTCNKTCLNYEWR-KDCKNFLCKNPLTEIFKNDTYILCAGKVETNCGST 1432          
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 1318.52 bits (3411), Expect = 0.000e+0
Identity = 738/739 (99.86%), Postives = 739/739 (100.00%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIXXXXXXXPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 2219
            EISFVIKLTPKSNT+VNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE
Sbjct:  344 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 1082          

HSP 2 Score: 101.293 bits (251), Expect = 1.859e-21
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            EISFVIKLTPKSNTEVNIVNLEINVNINSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  177 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 272          

HSP 3 Score: 72.0182 bits (175), Expect = 1.600e-12
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVT 2207
            ICT++FCSEYTC+ EC+  E R  +C+ L+C  R KE F+N+T I CTR  +ENC STS  +  +T
Sbjct: 6667 ICTYMFCSEYTCKGECVTGESRNVDCSKLICVGRSKEGFRNDTYIVCTRRIEENCVSTSTSSPVLT 6732          

HSP 4 Score: 68.9366 bits (167), Expect = 1.450e-11
Identity = 33/56 (58.93%), Postives = 42/56 (75.00%), Query Frame = 3
Query:  123 EIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            +I+    K  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:   50 QILSPVRKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 105          

HSP 5 Score: 64.6994 bits (156), Expect = 2.672e-10
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTS 2198
            ICT+++CSEYTC+ EC+  E R  +C+ LVC    +EA+KN+T I CT+  +ENC ST+ +++
Sbjct: 6506 ICTYMYCSEYTCKGECVTGESRNVDCSKLVCVGLSEEAYKNDTYIVCTKRIEENCISTTTRST 6568          

HSP 6 Score: 51.9878 bits (123), Expect = 2.639e-6
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKST 2183
            IC + +CS YTC   CLN E R ++C N +C + L E FKN+T I C    + NC ST
Sbjct: 1376 ICIYGYCSVYTCNKTCLNYEWR-KDCKNFLCKNPLTEIFKNDTYILCAGKVETNCGST 1432          
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 1318.52 bits (3411), Expect = 0.000e+0
Identity = 738/739 (99.86%), Postives = 739/739 (100.00%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIXXXXXXXPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 2219
            EISFVIKLTPKSNT+VNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE
Sbjct:  342 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 1080          

HSP 2 Score: 101.293 bits (251), Expect = 1.875e-21
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            EISFVIKLTPKSNTEVNIVNLEINVNINSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  175 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 270          

HSP 3 Score: 72.0182 bits (175), Expect = 1.600e-12
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVT 2207
            ICT++FCSEYTC+ EC+  E R  +C+ L+C  R KE F+N+T I CTR  +ENC STS  +  +T
Sbjct: 6665 ICTYMFCSEYTCKGECVTGESRNVDCSKLICVGRSKEGFRNDTYIVCTRRIEENCVSTSTSSPVLT 6730          

HSP 4 Score: 68.1662 bits (165), Expect = 3.004e-11
Identity = 32/49 (65.31%), Postives = 39/49 (79.59%), Query Frame = 3
Query:  144 KSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            K  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:   55 KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 103          

HSP 5 Score: 64.6994 bits (156), Expect = 2.672e-10
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTS 2198
            ICT+++CSEYTC+ EC+  E R  +C+ LVC    +EA+KN+T I CT+  +ENC ST+ +++
Sbjct: 6504 ICTYMYCSEYTCKGECVTGESRNVDCSKLVCVGLSEEAYKNDTYIVCTKRIEENCISTTTRST 6566          

HSP 6 Score: 51.9878 bits (123), Expect = 2.594e-6
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKST 2183
            IC + +CS YTC   CLN E R ++C N +C + L E FKN+T I C    + NC ST
Sbjct: 1374 ICIYGYCSVYTCNKTCLNYEWR-KDCKNFLCKNPLTEIFKNDTYILCAGKVETNCGST 1430          
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 1318.52 bits (3411), Expect = 0.000e+0
Identity = 738/739 (99.86%), Postives = 739/739 (100.00%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIXXXXXXXPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 2219
            EISFVIKLTPKSNT+VNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE
Sbjct:  342 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 1080          

HSP 2 Score: 101.293 bits (251), Expect = 1.843e-21
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            EISFVIKLTPKSNTEVNIVNLEINVNINSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  175 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 270          

HSP 3 Score: 72.0182 bits (175), Expect = 1.628e-12
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVT 2207
            ICT++FCSEYTC+ EC+  E R  +C+ L+C  R KE F+N+T I CTR  +ENC STS  +  +T
Sbjct: 6646 ICTYMFCSEYTCKGECVTGESRNVDCSKLICVGRSKEGFRNDTYIVCTRRIEENCVSTSTSSPVLT 6711          

HSP 4 Score: 68.1662 bits (165), Expect = 2.978e-11
Identity = 32/49 (65.31%), Postives = 39/49 (79.59%), Query Frame = 3
Query:  144 KSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            K  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:   55 KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 103          

HSP 5 Score: 65.0846 bits (157), Expect = 2.627e-10
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTS 2198
            ICT+++CSEYTC+ EC+  E R  +C+ LVC    +EA+KN+T I CT+  +ENC ST+ +++
Sbjct: 6485 ICTYMYCSEYTCKGECVTGESRNVDCSKLVCVGLSEEAYKNDTYIVCTKRIEENCISTTTRST 6547          

HSP 6 Score: 51.6026 bits (122), Expect = 2.661e-6
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKST 2183
            IC + +CS YTC   CLN E R ++C N +C + L E FKN+T I C    + NC ST
Sbjct: 1374 ICIYGYCSVYTCNKTCLNYEWR-KDCKNFLCKNPLTEIFKNDTYILCAGKVETNCGST 1430          
BLAST of SCO-spondin vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 1317.75 bits (3409), Expect = 0.000e+0
Identity = 738/739 (99.86%), Postives = 739/739 (100.00%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPXXXXXXXXXXXXXXXXXPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIXXXXXXXPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLXXXXXXXXXXXXXXXKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNXXXXXXXXXXXXXXXXXXXXXXXVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 2219
            EISFVIKLTPKSNT+VNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE
Sbjct:  344 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVSTAQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKTHKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCESMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRKNGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNITTTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRNGDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYKCDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCLGACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVSTTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGSICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLCKGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYVTITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISE 1082          

HSP 2 Score: 101.293 bits (251), Expect = 1.891e-21
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXXXXXXXFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            EISFVIKLTPKSNTEVNIVNLEINVNINSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  177 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 272          

HSP 3 Score: 72.0182 bits (175), Expect = 1.713e-12
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVT 2207
            ICT++FCSEYTC+ EC+  E R  +C+ L+C  R KE F+N+T I CTR  +ENC STS  +  +T
Sbjct: 6687 ICTYMFCSEYTCKGECVTGESRNVDCSKLICVGRSKEGFRNDTYIVCTRRIEENCVSTSTSSPVLT 6752          

HSP 4 Score: 68.9366 bits (167), Expect = 1.462e-11
Identity = 33/56 (58.93%), Postives = 42/56 (75.00%), Query Frame = 3
Query:  123 EIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 290
            +I+    K  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:   50 QILSPVRKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 105          

HSP 5 Score: 64.6994 bits (156), Expect = 2.788e-10
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKSTSKQTS 2198
            ICT+++CSEYTC+ EC+  E R  +C+ LVC    +EA+KN+T I CT+  +ENC ST+ +++
Sbjct: 6526 ICTYMYCSEYTCKGECVTGESRNVDCSKLVCVGLSEEAYKNDTYIVCTKRIEENCISTTTRST 6588          

HSP 6 Score: 51.6026 bits (122), Expect = 2.776e-6
Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 3
Query: 2010 ICTFVFCSEYTCENECLNQEGRAQNCNNLVCSSRLKEAFKNETVIFCTRIKQENCKST 2183
            IC + +CS YTC   CLN E R ++C N +C + L E FKN+T I C    + NC ST
Sbjct: 1376 ICIYGYCSVYTCNKTCLNYEWR-KDCKNFLCKNPLTEIFKNDTYILCAGKVETNCGST 1432          
The following BLAST results are available for this feature:
BLAST of SCO-spondin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8G0U96.837e-2326.66Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DLR68.945e-2325.77Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267GUZ74.124e-2226.02Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GY381.916e-1726.55Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A0L8FZQ87.223e-732.54Uncharacterized protein (Fragment) OS=Octopus bima... [more]
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BLAST of SCO-spondin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000000981.14.284e-634.25pep scaffold:Pmarinus_7.0:GL476384:615201:646381:-... [more]
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BLAST of SCO-spondin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCO-spondin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025545.11.859e-2199.86SMESG000025545.1[more]
SMESG000025545.11.859e-2199.86SMESG000025545.1[more]
SMESG000025545.11.875e-2199.86SMESG000025545.1[more]
SMESG000025545.11.843e-2199.86SMESG000025545.1[more]
SMESG000025545.11.891e-2199.86SMESG000025545.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032818 ID=SMED30032818|Name=SCO-spondin|organism=Schmidtea mediterranea sexual|type=transcript|length=2338bp
ATGAAATCAGCTTTGTTATCAAATTGACACCGAAATCAAACACAGAAGTG
AACATTGTCAACCTAGAAATCAATGTCAACATAAACTCAATAGTGACAAT
TACATTCGGAAAGAAGAGTGATGAAATCGTCGAATCACAATATAAATCAC
GGGGCTCAGAAAAATTTGAATTTAATTTCGACCAAACCGACAAAAACGGA
GAACAATTTGAATACATGAAAGTTTCGATTCGAACAAATGTTGGATATAC
TCTGACAGTCAAAAATCTGATAGTGAAAGTTTGCTATGAGAGTGTATCGA
CAGCTCAAACCTCTGCTCCTACTCAGGAAGTCACAACATCGGTTTGCAAG
GAAGACTCCACCGATATAATCACTAACTGTTTGATGAAAAAATGCATTAA
CGGGACTTGGACCCATCGTAAAGTCTGCTTACTGACCTGTTCTGAGAAAA
CTCATAAATTGGTTGAAAATGATAATCAATGCTGCGAATGTTTGCCTATA
TCCACAATTTCAATCCGAACCACCACGCCTGTAATGGCAACCACCACTCC
ATTCTGTAAACCACCATTAATTCACACATTCTGTGTAAAACCGACTTGTG
AATCCATGTGCGAAGCTCAACAACTCTCAAATTGTACGCGACAAGAGTGC
AAACCTGGGTGTGAGTGTCCTGAAAACCTGTTTTTCAACGGCAAGGATTG
TGTCTCAAGAAAAGAATGCATGTGCTTAGATGAAAATGGTGGAATTAGAA
AGAATGGTGAAGAATGGACAATAAATTGCAAGAATTATGTATGCGATGGT
ATTGTGACCAAACGAATATTCAATGATTGTCAAGGAGTATCTCTAAATTG
CCCCCCAGAATTCCAAATTATGGTAGATTGCTGCATGGTATGTAACATTA
CCACGACTACGGCAACTACTCCTCACATTTCTGTATGCAAATTTGAAAAT
CAAACTTTACAAGTTGGTGAAACAGTAATACGAAAAGTTGATTTCTGTGA
GGACGACACTTGCGTTTGCAACCAAGTTGGAGGATTAACCTGTACCAGAA
ATGGAGACAAACTCCGAAATTGCAAAGAAAGTTGCTCATCTAAAAAATGT
GACGGATTGATCGTACCGAGAAATGAAAAATGTATCTGCGAATGCAATTG
CTTGCCATCATCCAGTATTCCCCCCACTCCATGCTCAACATATAGCTACA
AGTGTGATTTGCCTTGCGACATCGGAGATTTGGAAAACCTGGAATTGAAT
CAGTTCAAAAAATATTGTAATCTCACCAATCTTATTTGCGGTGAATGCCC
ATCTACCGATTTTCGCTACATTGACAGTGCCACAGGAATTCAATACTGTC
TTGGCGCATGCTGTAAATTTAATGGAAAGAAAATTAAAGCCGGTGAAAGT
GTACCTCAATTAACAAATAATGGCACTGAGATTCCGTGTAGCTATTGCCA
ATGTCTCATTAATGGCAGATTTAAATGCATGAAAGATGACAACTGTGTAT
CAACAACTACAAAAACTGTGACTACTACATCTATCATTTGTCCAACTACA
TGTAAGAAAATCGTCGAAGAATCAGTTTTTCAAACTTGTAGAAAACAAAT
TTGCCTCAAATTTCAGGAAAATTGTTTGAAAGATGGCTTATGTGATGGAT
CAATATGCGAATGCAAAGAACCTCTTGTTTTTAATGGAACAGATTGCATC
AATGTCAAAGAATGCCCATGCTTAGATCCAATTACTAGAAAGCAATTTAA
AGTTGGAGAATCATGGATAGATGCAACGGACGAATGCAGAATTCATTTAT
GTAAAGGAAACGAAATAGAAACCAAAGAAATACGAAAAGAATGTCCCATA
TTAGAATGTCCAGAAGGGTGCATTAAAACCATTGTTGAAGAGAGAAATAA
ATGTTGTCCAATATGCTCATGTAATGAAACTACAACGTCTACAAAATATG
TCACAATTACACCAACTCAATCTTCGATATCAACAGCAATCACAACCAAA
CCACCAAATATTTGCACATTCGTGTTCTGTTCAGAGTATACTTGTGAAAA
TGAATGCCTAAATCAGGAAGGAAGAGCACAAAATTGTAATAATCTAGTCT
GTTCTAGTCGTTTAAAAGAAGCCTTTAAAAACGAAACCGTAATATTTTGT
ACTCGGATTAAACAAGAAAATTGTAAATCAACATCAAAACAAACAAGCAC
AGTTACAAATATTTCAGAAACAACCAAAACTTCAACCGTTTTTCCACCAA
CCATCACAACAACATCAACAGATGTTCCATTAACCACCACAACGATCTCA
ACAAGTACTCAAACGATCACCACAGCAACATCAACAGC
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protein sequence of SMED30032818-orf-1

>SMED30032818-orf-1 ID=SMED30032818-orf-1|Name=SMED30032818-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=778bp
EISFVIKLTPKSNTEVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSR
GSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYESVST
AQTSAPTQEVTTSVCKEDSTDIITNCLMKKCINGTWTHRKVCLLTCSEKT
HKLVENDNQCCECLPISTISIRTTTPVMATTTPFCKPPLIHTFCVKPTCE
SMCEAQQLSNCTRQECKPGCECPENLFFNGKDCVSRKECMCLDENGGIRK
NGEEWTINCKNYVCDGIVTKRIFNDCQGVSLNCPPEFQIMVDCCMVCNIT
TTTATTPHISVCKFENQTLQVGETVIRKVDFCEDDTCVCNQVGGLTCTRN
GDKLRNCKESCSSKKCDGLIVPRNEKCICECNCLPSSSIPPTPCSTYSYK
CDLPCDIGDLENLELNQFKKYCNLTNLICGECPSTDFRYIDSATGIQYCL
GACCKFNGKKIKAGESVPQLTNNGTEIPCSYCQCLINGRFKCMKDDNCVS
TTTKTVTTTSIICPTTCKKIVEESVFQTCRKQICLKFQENCLKDGLCDGS
ICECKEPLVFNGTDCINVKECPCLDPITRKQFKVGESWIDATDECRIHLC
KGNEIETKEIRKECPILECPEGCIKTIVEERNKCCPICSCNETTTSTKYV
TITPTQSSISTAITTKPPNICTFVFCSEYTCENECLNQEGRAQNCNNLVC
SSRLKEAFKNETVIFCTRIKQENCKSTSKQTSTVTNISETTKTSTVFPPT
ITTTSTDVPLTTTTISTSTQTITTATST
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0050840extracellular matrix binding
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007399nervous system development
GO:0030154cell differentiation
GO:0007155cell adhesion
GO:0007339binding of sperm to zona pellucida
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002075pigment cell
Vocabulary: cellular component
TermDefinition
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 172..286
e-value: 2.8E-9
score: 39.2