Serine/threonine-protein kinase DCLK3
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032800 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Match: DCLK3 (doublecortin like kinase 3 [Source:HGNC Symbol;Acc:HGNC:19005]) HSP 1 Score: 338.961 bits (868), Expect = 1.417e-102 Identity = 154/278 (55.40%), Postives = 210/278 (75.54%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLG--NSIRFSKPI 3194
+ EK+Y+ G IG GNF+ VKE + + T + +A KIIDK RLK KED+++ EI I++S HPNIVKL E +ET +++L+ EY++ GDLFDAI E++KFPE +A+M+ D+ +AL+++H K IVHRD+KPENLL ++ LKL+DFGLA + P++ +CGTPTYVAPEILSE+GYG+EVD+WA+GVI YILLCGFPPFRSPER+Q ELF I+ G FE++ PYWD IS +KDL++ +L+VDP KR+T L HPWI T G N+++ K +
Sbjct: 351 NVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKTNTVKRQKQV 628
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Match: DCLK3 (doublecortin like kinase 3 [Source:HGNC Symbol;Acc:HGNC:19005]) HSP 1 Score: 338.576 bits (867), Expect = 1.211e-100 Identity = 154/278 (55.40%), Postives = 210/278 (75.54%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLG--NSIRFSKPI 3194
+ EK+Y+ G IG GNF+ VKE + + T + +A KIIDK RLK KED+++ EI I++S HPNIVKL E +ET +++L+ EY++ GDLFDAI E++KFPE +A+M+ D+ +AL+++H K IVHRD+KPENLL ++ LKL+DFGLA + P++ +CGTPTYVAPEILSE+GYG+EVD+WA+GVI YILLCGFPPFRSPER+Q ELF I+ G FE++ PYWD IS +KDL++ +L+VDP KR+T L HPWI T G N+++ K +
Sbjct: 520 NVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKTNTVKRQKQV 797 HSP 2 Score: 89.3521 bits (220), Expect = 2.071e-17 Identity = 44/111 (39.64%), Postives = 74/111 (66.67%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGS-NWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEK---MSDHS 1046
KPR++T+++ G + +LLN+ S Q++E LL+DIS+A GS WK +++++N+ G+EI+ SDFFR G F+A+G K+P L + ++ V EL+PN + ++ HS
Sbjct: 93 KPRVVTVVKLGGQRPRKITLLLNRRSVQTFEQLLADISEALGSPRWKNDRVRKLFNLKGREIRSVSDFFREGDAFIAMG-KEP-LTLKSIQVAVEELYPNKARALTLAQHS 201
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 292.738 bits (748), Expect = 3.227e-85 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET ++FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T L HPW+
Sbjct: 393 YKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 650 HSP 2 Score: 72.0182 bits (175), Expect = 4.152e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T I+P + +G + + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNINPNWSVNIKGG--------------------TSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 270
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 293.123 bits (749), Expect = 3.858e-85 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET ++FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T L HPW+
Sbjct: 410 YKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 667 HSP 2 Score: 72.4034 bits (176), Expect = 3.765e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T I+P + +G + + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNINPNWSVNIKGG--------------------TSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 270
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002]) HSP 1 Score: 292.352 bits (747), Expect = 2.326e-84 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET ++FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T L HPW+
Sbjct: 393 YKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWV 650 HSP 2 Score: 72.4034 bits (176), Expect = 3.904e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T I+P + +G + + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNINPNWSVNIKGG--------------------TSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 270
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Match: cmk-1 (Calcium/calmodulin-dependent protein kinase type 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ0]) HSP 1 Score: 227.254 bits (578), Expect = 4.973e-66 Identity = 111/259 (42.86%), Postives = 163/259 (62.93%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKA-TGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLA-VEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y + +G+G FS+V A+ K+ G+ +A K IDKK LK KE+ +E EI++L+ H NIV+L + ++ V+LV E + G+LFD I + E ++ ++R + A+ ++H G+VHRD+KPENLL ++ + +SDFGL+ E + CGTP YVAPE+L ++ YG VD+W+ GVI YILLCG+PPF + + LF I GE+E+ PYWD+IS +KD I +++ DP RFT D L HPWI
Sbjct: 22 YDFRDVLGTGAFSKVFLAESKSDAGQMYAVKCIDKKALKGKEESLENEIKVLRKLRHNNIVQLFDTYDEKQFVYLVMELVTGGELFDRIVAKGSYTEQDASNLIRQVLEAVGFMHDNGVVHRDLKPENLLYYNQDEDSKIMISDFGLSKTEDSGVMATACGTPGYVAPEVLQQKPYGKAVDVWSIGVIAYILLCGYPPFY--DESDANLFAQIIKGEYEFDAPYWDQISDSAKDFITHLMCCDPEARFTCQDALSHPWI 278
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 214.927 bits (546), Expect = 1.136e-61 Identity = 111/264 (42.05%), Postives = 161/264 (60.98%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE-DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEI--KEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
F Y + +G G FS V+ K TG FAAKII+ K+L ++ +E E RI + +HPNIV+L + + + +LV + + G+LF+ I + E+ ++ ++ I ++ Y HS GIVHRD+KPENLL + K +KL+DFGLA+E+ E + GTP Y++PE+L + Y VDIWA GVI YILL G+PPF + +Q L+ IKAG ++Y P WD ++PE+K LI++ML V+P KR T L PWI
Sbjct: 8 FSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWI 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 214.927 bits (546), Expect = 1.136e-61 Identity = 111/264 (42.05%), Postives = 161/264 (60.98%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE-DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEI--KEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
F Y + +G G FS V+ K TG FAAKII+ K+L ++ +E E RI + +HPNIV+L + + + +LV + + G+LF+ I + E+ ++ ++ I ++ Y HS GIVHRD+KPENLL + K +KL+DFGLA+E+ E + GTP Y++PE+L + Y VDIWA GVI YILL G+PPF + +Q L+ IKAG ++Y P WD ++PE+K LI++ML V+P KR T L PWI
Sbjct: 8 FSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWI 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 214.927 bits (546), Expect = 1.136e-61 Identity = 111/264 (42.05%), Postives = 161/264 (60.98%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE-DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEI--KEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
F Y + +G G FS V+ K TG FAAKII+ K+L ++ +E E RI + +HPNIV+L + + + +LV + + G+LF+ I + E+ ++ ++ I ++ Y HS GIVHRD+KPENLL + K +KL+DFGLA+E+ E + GTP Y++PE+L + Y VDIWA GVI YILL G+PPF + +Q L+ IKAG ++Y P WD ++PE+K LI++ML V+P KR T L PWI
Sbjct: 8 FSDNYDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGIVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFW--DEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWI 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Match: F32D8.1 (pep chromosome:WBcel235:V:10880837:10883399:1 gene:WBGene00009326.1 transcript:F32D8.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F32D8.1) HSP 1 Score: 214.157 bits (544), Expect = 1.158e-61 Identity = 105/269 (39.03%), Postives = 163/269 (60.59%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Q+++ +D EK Y++G IG GNFS V ++K G A K ++K+ ++ K ++ E+ +L +H +I+ + + F T N+ F+V E+ + GDL++ I +N + E +A++ + AL Y+H + +VHRD+KPENLL K ++KL DFGLA I PLY ICGTPTY APE+L E GY DIW+ GV+ Y++L G+ PFR+P+ QT LF+ I + P W IS ++KDL++ ++ +R + HPWI
Sbjct: 59 QRVDGRQDIEKQYEIGAIIGKGNFSSVHLTRRKEDGTKCALKQVEKRAMRGKCFFVDNEVEMLSLIQHDHIISIIDAFSTENQYFIVFEHAQYGDLYETIRKNGRIEEPDAAIITLQVASALNYLHERNVVHRDVKPENLLLVDK---FSVKLCDFGLACHILGPLYRICGTPTYCAPEVLRETGYSTRCDIWSLGVVLYVMLVGYAPFRAPD--QTRLFKLIMQAKPNLDMPEWKGISLKAKDLVSRLMNKTEERRPLASQIMAHPWI 322
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Match: CG17528 (gene:FBgn0261387 transcript:FBtr0111274) HSP 1 Score: 283.878 bits (725), Expect = 8.086e-82 Identity = 135/273 (49.45%), Postives = 181/273 (66.30%), Query Frame = 3
Query: 2358 ETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI--KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGN 3170
E + Y LG IG GNF+ V + K + TG +A KIIDK + K KE I+ E+R++K HP+I+ L + ++LV EY+ GDLFDAI + +F E+QS +M+R + A+ Y+HS GIVHRDIKPENLL + ++ LKL+DFGLA E+ + LY +CGTPTYVAPEIL E GYG+++D+WA+G+I YILLCGFPPF +P+ Q LF+ I +G +E+ PYW +I +DLI NML DP RFT D L H W T+GN
Sbjct: 468 ELPSNIRNTYSLGRIIGDGNFAIVFKIKHRQTGHSYALKIIDKNKCKGKEHYIDAEVRVMKKLNHPHIISLILSVDQNTNMYLVLEYVSGGDLFDAITQVTRFSENQSRIMIRHLGAAMTYLHSMGIVHRDIKPENLLVKLDEHGNVLELKLADFGLACEVNDLLYAVCGTPTYVAPEILLEVGYGLKIDVWAAGIILYILLCGFPPFVAPDNQQEPLFDAIISGIYEFPDPYWSDIGDGVRDLIANMLQADPDVRFTSEDILDHSW--TIGN 738 HSP 2 Score: 81.6481 bits (200), Expect = 2.523e-15 Identity = 76/279 (27.24%), Postives = 117/279 (41.94%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGGQVQDIDDLEHRNIYIC-CGQESLKTYDYDTKYFELIHKGHLHN--HNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGD------KDPNLNQEEVRNI 1001
RK R+ F NG+R + G + ++ RY L +DLT+ IP VR IY+ G ++ +D+LE Y+C C E+ K +Y+T + + L N NSH P N S P G +RL +R PRI+T+IR+G KP ++LLNK +S S++ +L+ I+ +++++ + G + + SDFF S F A G +D L EE R I
Sbjct: 155 RKALRIKFYRNGDRFYPGITIPVSNERYRSFERLFEDLTRLLEENVKIPGAVRTIYNLCGKKITSLDELEDGQSYVCSCNNENFKKVEYNTGS-QPLSNLPLSNSRSNSHRLAKCRPSSPLKNGLLAGSSPFPACGGGT---------------GNGSPLIASRLSDRV---------TVVHPRIVTLIRSGTKPRRIMRLLLNKRNSPSFDHVLTAITQVV--RLDTGYVRKVFTLSGIPVVRLSDFFGSDDVFFAYGTERINTAEDFKLEAEEQRAI 406
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Match: CG17528 (gene:FBgn0261387 transcript:FBtr0111277) HSP 1 Score: 283.878 bits (725), Expect = 8.086e-82 Identity = 135/273 (49.45%), Postives = 181/273 (66.30%), Query Frame = 3
Query: 2358 ETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI--KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGN 3170
E + Y LG IG GNF+ V + K + TG +A KIIDK + K KE I+ E+R++K HP+I+ L + ++LV EY+ GDLFDAI + +F E+QS +M+R + A+ Y+HS GIVHRDIKPENLL + ++ LKL+DFGLA E+ + LY +CGTPTYVAPEIL E GYG+++D+WA+G+I YILLCGFPPF +P+ Q LF+ I +G +E+ PYW +I +DLI NML DP RFT D L H W T+GN
Sbjct: 468 ELPSNIRNTYSLGRIIGDGNFAIVFKIKHRQTGHSYALKIIDKNKCKGKEHYIDAEVRVMKKLNHPHIISLILSVDQNTNMYLVLEYVSGGDLFDAITQVTRFSENQSRIMIRHLGAAMTYLHSMGIVHRDIKPENLLVKLDEHGNVLELKLADFGLACEVNDLLYAVCGTPTYVAPEILLEVGYGLKIDVWAAGIILYILLCGFPPFVAPDNQQEPLFDAIISGIYEFPDPYWSDIGDGVRDLIANMLQADPDVRFTSEDILDHSW--TIGN 738 HSP 2 Score: 81.6481 bits (200), Expect = 2.523e-15 Identity = 76/279 (27.24%), Postives = 117/279 (41.94%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGGQVQDIDDLEHRNIYIC-CGQESLKTYDYDTKYFELIHKGHLHN--HNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGD------KDPNLNQEEVRNI 1001
RK R+ F NG+R + G + ++ RY L +DLT+ IP VR IY+ G ++ +D+LE Y+C C E+ K +Y+T + + L N NSH P N S P G +RL +R PRI+T+IR+G KP ++LLNK +S S++ +L+ I+ +++++ + G + + SDFF S F A G +D L EE R I
Sbjct: 155 RKALRIKFYRNGDRFYPGITIPVSNERYRSFERLFEDLTRLLEENVKIPGAVRTIYNLCGKKITSLDELEDGQSYVCSCNNENFKKVEYNTGS-QPLSNLPLSNSRSNSHRLAKCRPSSPLKNGLLAGSSPFPACGGGT---------------GNGSPLIASRLSDRV---------TVVHPRIVTLIRSGTKPRRIMRLLLNKRNSPSFDHVLTAITQVV--RLDTGYVRKVFTLSGIPVVRLSDFFGSDDVFFAYGTERINTAEDFKLEAEEQRAI 406
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0333670) HSP 1 Score: 246.128 bits (627), Expect = 3.923e-72 Identity = 130/290 (44.83%), Postives = 181/290 (62.41%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKAT-GKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLA-VEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTE 3212
++L E+ Y L +G+G FSEV+ A+ K + G+ FA KIIDKK LK KE+ +E EIR+L+ HPNIV+L E +E +KV+LV E + G+LFD I E + E ++ ++R I A+ Y+H +G+VHRD+KPENLL + + +SDFGL+ +E + CGTP YVAPE+L+++ YG VD+W+ GVI YILLCG+PPF + N LF I G+FE+ PYWDEIS +K I N++ V KR+T L H WI GN S+ I V+E
Sbjct: 19 KELNKQVSIEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKALKGKEESLENEIRVLRRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCGYPPFY--DENDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWIS--GNEAS-SRNIHGTVSE 303
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0089067) HSP 1 Score: 236.113 bits (601), Expect = 2.397e-68 Identity = 131/305 (42.95%), Postives = 182/305 (59.67%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKAT-GKPFAAKIIDKKRLKDKEDLIEEEIRILK---------SC------EHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLA-VEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTE 3212
++L E+ Y L +G+G FSEV+ A+ K + G+ FA KIIDKK LK KE+ +E EIR+L+ C HPNIV+L E +E +KV+LV E + G+LFD I E + E ++ ++R I A+ Y+H +G+VHRD+KPENLL + + +SDFGL+ +E + CGTP YVAPE+L+++ YG VD+W+ GVI YILLCG+PPF + N LF I G+FE+ PYWDEIS +K I N++ V KR+T L H WI GN S+ I V+E
Sbjct: 19 KELNKQVSIEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKALKGKEESLENEIRVLRRFSANHFDGKCLNGTRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCGYPPFY--DENDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWIS--GNEAS-SRNIHGTVSE 318
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0089065) HSP 1 Score: 236.113 bits (601), Expect = 2.397e-68 Identity = 131/305 (42.95%), Postives = 182/305 (59.67%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKAT-GKPFAAKIIDKKRLKDKEDLIEEEIRILK---------SC------EHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLA-VEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTE 3212
++L E+ Y L +G+G FSEV+ A+ K + G+ FA KIIDKK LK KE+ +E EIR+L+ C HPNIV+L E +E +KV+LV E + G+LFD I E + E ++ ++R I A+ Y+H +G+VHRD+KPENLL + + +SDFGL+ +E + CGTP YVAPE+L+++ YG VD+W+ GVI YILLCG+PPF + N LF I G+FE+ PYWDEIS +K I N++ V KR+T L H WI GN S+ I V+E
Sbjct: 19 KELNKQVSIEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKALKGKEESLENEIRVLRRFSANHFDGKCLNGTRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCGYPPFY--DENDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWIS--GNEAS-SRNIHGTVSE 318
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 302.368 bits (773), Expect = 6.969e-89 Identity = 148/302 (49.01%), Postives = 202/302 (66.89%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISK--NTS 3281
Y++G IG GNF+ VKE +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++VM+ D+ AL Y+H IVHRDIKPENLL C NG +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G E+ P+WD IS +K+LI +ML V+ R+T D L HPW+ +D E +++++ + K N++ K NTS
Sbjct: 392 YKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWV------------TEDAAMENNMKMEVAGKLKKHFNSVQKQSNTS 681 HSP 2 Score: 101.293 bits (251), Expect = 2.644e-21 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
S++ + +++ + +T + +K K+V F NG++ F G + +++ +R+ + LL +LT+ S+ + P GVR IY +GG+ + +DDL Y+C E + DY NP + N + S P + N L ER K I KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA +++R+Y ++GK+I DFF F+A G
Sbjct: 43 SAHCSFYRTRTLQSLTSE-----KKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNV--------------------NPN-WSVNVKTGASRSMPSL-----------------------TATKNELRERESKDYI-------KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACG 264
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 303.138 bits (775), Expect = 7.665e-89 Identity = 148/302 (49.01%), Postives = 202/302 (66.89%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISK--NTS 3281
Y++G IG GNF+ VKE +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++VM+ D+ AL Y+H IVHRDIKPENLL C NG +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G E+ P+WD IS +K+LI +ML V+ R+T D L HPW+ +D E +++++ + K N++ K NTS
Sbjct: 420 YKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWV------------TEDAAMENNMKMEVAGKLKKHFNSVQKQSNTS 709 HSP 2 Score: 101.293 bits (251), Expect = 2.257e-21 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
S++ + +++ + +T + +K K+V F NG++ F G + +++ +R+ + LL +LT+ S+ + P GVR IY +GG+ + +DDL Y+C E + DY NP + N + S P + N L ER K I KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA +++R+Y ++GK+I DFF F+A G
Sbjct: 43 SAHCSFYRTRTLQSLTSE-----KKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNV--------------------NPN-WSVNVKTGASRSMPSL-----------------------TATKNELRERESKDYI-------KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACG 264
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 302.368 bits (773), Expect = 7.687e-89 Identity = 148/302 (49.01%), Postives = 202/302 (66.89%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISK--NTS 3281
Y++G IG GNF+ VKE +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++VM+ D+ AL Y+H IVHRDIKPENLL C NG +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G E+ P+WD IS +K+LI +ML V+ R+T D L HPW+ +D E +++++ + K N++ K NTS
Sbjct: 401 YKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWV------------TEDAAMENNMKMEVAGKLKKHFNSVQKQSNTS 690 HSP 2 Score: 100.908 bits (250), Expect = 2.816e-21 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
S++ + +++ + +T + +K K+V F NG++ F G + +++ +R+ + LL +LT+ S+ + P GVR IY +GG+ + +DDL Y+C E + DY NP + N + S P + N L ER K I KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA +++R+Y ++GK+I DFF F+A G
Sbjct: 43 SAHCSFYRTRTLQSLTSE-----KKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNV--------------------NPN-WSVNVKTGASRSMPSL-----------------------TATKNELRERESKDYI-------KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACG 264
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 302.368 bits (773), Expect = 7.687e-89 Identity = 148/302 (49.01%), Postives = 202/302 (66.89%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISK--NTS 3281
Y++G IG GNF+ VKE +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++VM+ D+ AL Y+H IVHRDIKPENLL C NG +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G E+ P+WD IS +K+LI +ML V+ R+T D L HPW+ +D E +++++ + K N++ K NTS
Sbjct: 401 YKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWV------------TEDAAMENNMKMEVAGKLKKHFNSVQKQSNTS 690 HSP 2 Score: 100.908 bits (250), Expect = 2.816e-21 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
S++ + +++ + +T + +K K+V F NG++ F G + +++ +R+ + LL +LT+ S+ + P GVR IY +GG+ + +DDL Y+C E + DY NP + N + S P + N L ER K I KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA +++R+Y ++GK+I DFF F+A G
Sbjct: 43 SAHCSFYRTRTLQSLTSE-----KKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNV--------------------NPN-WSVNVKTGASRSMPSL-----------------------TATKNELRERESKDYI-------KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACG 264
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170]) HSP 1 Score: 302.368 bits (773), Expect = 1.215e-88 Identity = 148/302 (49.01%), Postives = 202/302 (66.89%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISK--NTS 3281
Y++G IG GNF+ VKE +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++VM+ D+ AL Y+H IVHRDIKPENLL C NG +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G E+ P+WD IS +K+LI +ML V+ R+T D L HPW+ +D E +++++ + K N++ K NTS
Sbjct: 417 YKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWV------------TEDAAMENNMKMEVAGKLKKHFNSVQKQSNTS 706 HSP 2 Score: 100.908 bits (250), Expect = 2.951e-21 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
S++ + +++ + +T + +K K+V F NG++ F G + +++ +R+ + LL +LT+ S+ + P GVR IY +GG+ + +DDL Y+C E + DY NP + N + S P + N L ER K I KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA +++R+Y ++GK+I DFF F+A G
Sbjct: 43 SAHCSFYRTRTLQSLTSE-----KKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNV--------------------NPN-WSVNVKTGASRSMPSL-----------------------TATKNELRERESKDYI-------KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACG 264
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Match: ovch1 (ovochymase 1 [Source:Xenbase;Acc:XB-GENE-6449526]) HSP 1 Score: 316.235 bits (809), Expect = 2.090e-93 Identity = 146/265 (55.09%), Postives = 199/265 (75.09%), Query Frame = 3
Query: 2382 YYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK-NGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 3173
YY++G +IG GNF+ VK + + +A KIIDK +L+ KED+IE E+RI+K HPNIVKL +ET +++L+ EYI+ GDLFD I E+IKF E +A+M+ D+ AL YIHSK IVHRD+KPENLL +G +LKL+DFGLA+ P++ +CGTPTYVAPEILSE+GYG++VD+WA+GVI YILLCGFPPFRSP+RNQ ELF+ I++GE+E++ PYWD IS E KDLI+ +L+++P R++ L H W+ + G +
Sbjct: 428 YYEIGRTIGDGNFAVVKVCRPWNENQEYAMKIIDKSKLRGKEDIIENEVRIIKHLSHPNIVKLFSDYETEKEIYLILEYIRGGDLFDVITESIKFTEHDAALMLIDLCEALKYIHSKHIVHRDLKPENLLVQHNPDGSKSLKLADFGLAIYATGPIFTVCGTPTYVAPEILSEKGYGLKVDMWATGVILYILLCGFPPFRSPDRNQEELFQIIQSGEYEFLPPYWDHISEEVKDLISKLLVLNPEIRYSAKCVLQHSWVTSRGQT 692 HSP 2 Score: 83.9593 bits (206), Expect = 8.486e-16 Identity = 47/123 (38.21%), Postives = 73/123 (59.35%), Query Frame = 3
Query: 702 IAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFG-SNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSAD-DSRL 1064
I N KPRI+T+++ G P +LLNK S Q++E L++DIS+A G WK ++++YN+ GKEI+ SDFFR F+A+G + L + ++ EL+P E+ + S D D +L
Sbjct: 30 IHSENSPVKPRIVTVVKPGSHPLKKITLLLNKRSVQTFEQLVADISEALGFPRWKNDRVRKLYNLRGKEIRSISDFFRGDDIFIALGRE--KLTTKCTESVFNELYP--ERSAKKSKDQDGKL 148
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257]) HSP 1 Score: 280.411 bits (716), Expect = 3.794e-84 Identity = 136/286 (47.55%), Postives = 193/286 (67.48%), Query Frame = 3
Query: 2319 LSQRIVPVLAQQLETDEDFE------KYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
L +PV+ + DE F+ + Y++G +IG GNF+ VKE +++TG+ +A KII+K + + KE +I+ E+ IL+ +HPNIV L E+ + P++++LV E +K GDLFDAI K+ E + M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G ++ PYWD +S +K+LI ML VD +R++ L L HPW+
Sbjct: 31 LKATYLPVICKADIMDEGFQVPVSISERYKVGRTIGDGNFAIVKECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGHMDFPSPYWDNVSDSAKELITMMLQVDVDQRYSALKVLEHPWV 316
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257]) HSP 1 Score: 280.026 bits (715), Expect = 8.297e-84 Identity = 135/279 (48.39%), Postives = 188/279 (67.38%), Query Frame = 3
Query: 2322 SQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
S R VPV + Y++G +IG GNF+ VKE +++TG+ +A KII+K + + KE +I+ E+ IL+ +HPNIV L E+ + P++++LV E +K GDLFDAI K+ E + M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G ++ PYWD +S +K+LI ML VD +R++ L L HPW+
Sbjct: 53 SARSVPV---------SISERYKVGRTIGDGNFAIVKECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGHMDFPSPYWDNVSDSAKELITMMLQVDVDQRYSALKVLEHPWV 322
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257]) HSP 1 Score: 277.715 bits (709), Expect = 1.204e-83 Identity = 130/258 (50.39%), Postives = 182/258 (70.54%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G +IG GNF+ VKE +++TG+ +A KII+K + + KE +I+ E+ IL+ +HPNIV L E+ + P++++LV E +K GDLFDAI K+ E + M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G ++ PYWD +S +K+LI ML VD +R++ L L HPW+
Sbjct: 50 YKVGRTIGDGNFAIVKECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGHMDFPSPYWDNVSDSAKELITMMLQVDVDQRYSALKVLEHPWV 307
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257]) HSP 1 Score: 280.026 bits (715), Expect = 1.262e-83 Identity = 130/258 (50.39%), Postives = 182/258 (70.54%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G +IG GNF+ VKE +++TG+ +A KII+K + + KE +I+ E+ IL+ +HPNIV L E+ + P++++LV E +K GDLFDAI K+ E + M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G ++ PYWD +S +K+LI ML VD +R++ L L HPW+
Sbjct: 83 YKVGRTIGDGNFAIVKECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGHMDFPSPYWDNVSDSAKELITMMLQVDVDQRYSALKVLEHPWV 340
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Match: Dclk3 (doublecortin-like kinase 3 [Source:MGI Symbol;Acc:MGI:3039580]) HSP 1 Score: 343.969 bits (881), Expect = 5.309e-103 Identity = 154/269 (57.25%), Postives = 208/269 (77.32%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 3173
D EK+Y +G IG GNF+ VKE + + T + +A K+IDK +LK KED+++ EI I++S HPNIVKL E +ET +++L+ EY++ GDLFDAI EN+KFPE ++AVM+ D+ +AL+++H K IVHRD+KPENLL ++ I LKL+DFGLA + P++ +CGTPTYVAPEILSE+GYG+EVD+WA+GVI YILLCGFPPFRSPER+Q ELF I+ G+FE++ PYWD IS +KDL+ N+L VDP KR+T L HPWI +G++
Sbjct: 509 DVEKHYDIGGVIGDGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQSLSHPNIVKLHEVYETEAEIYLIMEYVQGGDLFDAIVENVKFPEPEAAVMITDLCKALVHMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLAKYVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFEFLSPYWDNISDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHT 777 HSP 2 Score: 85.8853 bits (211), Expect = 1.994e-16 Identity = 39/105 (37.14%), Postives = 69/105 (65.71%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFG-SNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMS 1037
KPR++T+++ G +P +LLN+ S Q++E LLSDIS+A G WK +++++ + G+E++ SDFFR G F+A+G K+P L + ++ + EL+P ++
Sbjct: 95 KPRVVTVVKLGGQPLRKATLLLNRRSVQTFEQLLSDISEALGFPRWKNDRVRKLFTLKGREVKSVSDFFREGDAFIAMG-KEP-LTLKSIQLAMEELYPKNRALA 197
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 293.123 bits (749), Expect = 2.782e-85 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE + TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET +FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+HS IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VD+WA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T + L HPW+
Sbjct: 409 YRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 666 HSP 2 Score: 73.1738 bits (178), Expect = 1.606e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T ++P + +G L + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNVNPNWSVNIKGG---------------------TTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 292.738 bits (748), Expect = 1.055e-84 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE + TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET +FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+HS IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VD+WA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T + L HPW+
Sbjct: 392 YRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 649 HSP 2 Score: 73.1738 bits (178), Expect = 1.538e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T ++P + +G L + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNVNPNWSVNIKGG---------------------TTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Match: Dclk2 (doublecortin-like kinase 2 [Source:MGI Symbol;Acc:MGI:1918012]) HSP 1 Score: 292.738 bits (748), Expect = 1.066e-84 Identity = 139/258 (53.88%), Postives = 180/258 (69.77%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE + TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET +FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+HS IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VD+WA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T + L HPW+
Sbjct: 393 YRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 650 HSP 2 Score: 73.1738 bits (178), Expect = 1.501e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T ++P + +G L + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNVNPNWSVNIKGG---------------------TTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861]) HSP 1 Score: 280.796 bits (717), Expect = 1.444e-84 Identity = 129/258 (50.00%), Postives = 183/258 (70.93%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G +IG GNF+ VKE +++T + +A KII K + + KE +I+ E+ IL+ +HPNIV L E+ + P +++LV E +K GDLFDAI K+ E ++ M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G+ ++ PYWD +S +K+LIN ML+V+ +RF+ + L HPW+
Sbjct: 83 YKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWV 340
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Match: sp|Q8BWQ5|DCLK3_MOUSE (Serine/threonine-protein kinase DCLK3 OS=Mus musculus OX=10090 GN=Dclk3 PE=1 SV=2) HSP 1 Score: 343.969 bits (881), Expect = 3.714e-102 Identity = 154/269 (57.25%), Postives = 208/269 (77.32%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 3173
D EK+Y +G IG GNF+ VKE + + T + +A K+IDK +LK KED+++ EI I++S HPNIVKL E +ET +++L+ EY++ GDLFDAI EN+KFPE ++AVM+ D+ +AL+++H K IVHRD+KPENLL ++ I LKL+DFGLA + P++ +CGTPTYVAPEILSE+GYG+EVD+WA+GVI YILLCGFPPFRSPER+Q ELF I+ G+FE++ PYWD IS +KDL+ N+L VDP KR+T L HPWI +G++
Sbjct: 509 DVEKHYDIGGVIGDGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQSLSHPNIVKLHEVYETEAEIYLIMEYVQGGDLFDAIVENVKFPEPEAAVMITDLCKALVHMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLAKYVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFEFLSPYWDNISDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHT 777 HSP 2 Score: 85.8853 bits (211), Expect = 1.394e-15 Identity = 39/105 (37.14%), Postives = 69/105 (65.71%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFG-SNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMS 1037
KPR++T+++ G +P +LLN+ S Q++E LLSDIS+A G WK +++++ + G+E++ SDFFR G F+A+G K+P L + ++ + EL+P ++
Sbjct: 95 KPRVVTVVKLGGQPLRKATLLLNRRSVQTFEQLLSDISEALGFPRWKNDRVRKLFTLKGREVKSVSDFFREGDAFIAMG-KEP-LTLKSIQLAMEELYPKNRALA 197
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Match: sp|Q9C098|DCLK3_HUMAN (Serine/threonine-protein kinase DCLK3 OS=Homo sapiens OX=9606 GN=DCLK3 PE=2 SV=2) HSP 1 Score: 338.961 bits (868), Expect = 6.804e-102 Identity = 154/278 (55.40%), Postives = 210/278 (75.54%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLG--NSIRFSKPI 3194
+ EK+Y+ G IG GNF+ VKE + + T + +A KIIDK RLK KED+++ EI I++S HPNIVKL E +ET +++L+ EY++ GDLFDAI E++KFPE +A+M+ D+ +AL+++H K IVHRD+KPENLL ++ LKL+DFGLA + P++ +CGTPTYVAPEILSE+GYG+EVD+WA+GVI YILLCGFPPFRSPER+Q ELF I+ G FE++ PYWD IS +KDL++ +L+VDP KR+T L HPWI T G N+++ K +
Sbjct: 351 NVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDLKPENLLVQRNEDKSTTLKLADFGLAKHVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKTNTVKRQKQV 628
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Match: sp|Q5MPA9|DCLK2_RAT (Serine/threonine-protein kinase DCLK2 OS=Rattus norvegicus OX=10116 GN=Dclk2 PE=1 SV=2) HSP 1 Score: 294.278 bits (752), Expect = 2.114e-84 Identity = 138/258 (53.49%), Postives = 181/258 (70.16%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET ++FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VD+WA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T + L HPW+
Sbjct: 409 YRIGKVIGDGNFAVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 666 HSP 2 Score: 74.7146 bits (182), Expect = 3.317e-12 Identity = 63/258 (24.42%), Postives = 108/258 (41.86%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T ++P + +G L + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGV-------------------RTIYTVDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNVNPNWSVNIKGG---------------------TTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 269
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Match: sp|D2I3C6|DCLK2_AILME (Serine/threonine-protein kinase DCLK2 OS=Ailuropoda melanoleuca OX=9646 GN=DCLK2 PE=3 SV=1) HSP 1 Score: 294.278 bits (752), Expect = 3.791e-84 Identity = 139/258 (53.88%), Postives = 181/258 (70.16%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++TGK FA KIIDK + KE LIE E+ IL+ +HPNI+ L E+ ET ++FLV E +K GDLFDAI + K+ E + M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I AG+ E+ PYWD I+ +K+LI+ ML V+ R T + L HPW+
Sbjct: 411 YKIGKVIGDGNFAVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV 668 HSP 2 Score: 72.7886 bits (177), Expect = 1.538e-11 Identity = 63/258 (24.42%), Postives = 110/258 (42.64%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADST--ISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
+K K+ F NG+R F G + +I+ +R+ + LL +LT+ S+ + P GV R IY G + + D L S++ P F D T I+P + +G +++ + K + E+ KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI++A ++R+ +DGK++ DFF F+A G
Sbjct: 69 KKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLMELTRSLSDNVNLPQGV-------------------RTIYTIDGSRKVTSLD------------ELLEGESYVCASNEP---FRKVDYTKNINPNWSVNIKGGTARALAAPS--------------------SVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAI--KLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACG 270
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Match: sp|O08875|DCLK1_RAT (Serine/threonine-protein kinase DCLK1 OS=Rattus norvegicus OX=10116 GN=Dclk1 PE=1 SV=1) HSP 1 Score: 283.493 bits (724), Expect = 4.122e-84 Identity = 133/273 (48.72%), Postives = 190/273 (69.60%), Query Frame = 3
Query: 2358 ETDEDFE------KYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
E+DE F+ + Y++G +IG GNF+ VKE +++T + +A KII K + + KE +I+ E+ IL+ +HPNIV L E+ + P +++LV E +K GDLFDAI K+ E ++ M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G+ ++ PYWD +S +K+LIN ML+V+ +RF+ + L HPW+
Sbjct: 68 ESDEGFQIPATITERYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWV 340
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Match: K1S2X4 (Serine/threonine-protein kinase DCLK3 OS=Crassostrea gigas OX=29159 GN=CGI_10028458 PE=4 SV=1) HSP 1 Score: 373.244 bits (957), Expect = 1.606e-110 Identity = 181/352 (51.42%), Postives = 247/352 (70.17%), Query Frame = 3
Query: 2226 PRSETVNLFMKGKSFQKPTRTK---------KQSSVLQNPLSQRIV--PVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIR--FSKPIKDVVTETRVELQMET 3239
PR + + K KS + P TK K+ + + +IV L +Q+ DE Y LG ++G GNF+ V+++K KA +A K+IDK +L+ KE ++E EI I+K C H NIVKL E++ET +K++LV E +K GDLFDAI +++KF E SA M++D+ AL Y+HS+ IVHRD+KPENLL K+G I+LKL+DFGLA+E+KEP++ +CGTPTYVAPEILSE GYG+EVD+WA GVI YILLCGFPPFRSP+RNQTELFEFIKAGE+E++ PYWD ISP +KDLI ++L+VD KR+T ++ L HPWI+ G+ R S + ++ TR EL+++
Sbjct: 419 PRDQKADAVTKEKSPRDPKATKEKTDPALKDKEKAEKKRKSKSKIVRKSKLERQVSGDEHITNKYDLGRTLGDGNFAIVRQSKLKAGSGEYAMKVIDKPKLRGKEHMVENEIEIMKDCHHHNIVKLYEEYETSDKIYLVMELVKGGDLFDAITQSVKFGEVDSARMVKDLCNALFYLHSRSIVHRDLKPENLLVHRNKDGSISLKLADFGLAMEVKEPIFTVCGTPTYVAPEILSEIGYGLEVDMWAVGVITYILLCGFPPFRSPDRNQTELFEFIKAGEYEFLSPYWDNISPYAKDLIEHLLVVDKKKRYTAIETLSHPWILLNGDMSRPPESSKLDEMRKNTRKELELQA 770 HSP 2 Score: 97.4413 bits (241), Expect = 9.596e-17 Identity = 53/148 (35.81%), Postives = 91/148 (61.49%), Query Frame = 3
Query: 714 NMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSPDASY 1157
N+ KP+++TI+R G KP + KILLN+ S QS+E L++DIS+AFG +K ++++++I G+E+Q SDFFR F+ +G + +L + ++ +IV EL+P+ S + R ++ NK K +N+ +T DH D+ +
Sbjct: 91 NIPLKPKVVTIVRNGHKPRNNVKILLNRRSVQSFEQLMADISEAFGPKYKNNKVKKLFSIRGREVQGISDFFRDDDVFIGVGGE--SLTETDIVDIVEELYPD----SSMAKSILRELERNKRKRHNL---AKQTQADHDADKRDSGF 229
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Match: A0A210QQR8 (Serine/threonine-protein kinase DCLK3 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04931 PE=4 SV=1) HSP 1 Score: 366.696 bits (940), Expect = 1.823e-107 Identity = 172/297 (57.91%), Postives = 226/297 (76.09%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGN--SIRFSKPIKDVVTETRVELQ 3230
+Q+ D Y LG ++G GNF+ VK+A K T FA KIIDK +LK KE ++E EI I+K C+HPNIVKL E++ET +K++LV E +K GDLFDAI +++KF E SA M++D+ AL Y+HS+ IVHRD+KPENLL K+ I LKL+DFGLA+++K+P+Y +CGTPTYVAPEILSE GYG+EVD+WA GVICYILLCGFPPFRS +RNQTELFEFIKAGE+E++ PYWD+IS +KDLI ++LIV+ KRFT +D L HPWI+ G+ I + ++ + T+TR EL+
Sbjct: 539 RQVSGDSHILSKYDLGKTLGDGNFAIVKQATLKNTKNEFAMKIIDKAKLKGKEHMVENEIEIMKDCQHPNIVKLYEEYETIDKIYLVMELVKGGDLFDAITQSVKFGEVDSAHMVKDLCNALFYLHSRSIVHRDLKPENLLVHRNKDSTITLKLADFGLAMDVKDPIYTVCGTPTYVAPEILSEIGYGLEVDMWAVGVICYILLCGFPPFRSADRNQTELFEFIKAGEYEFLSPYWDQISRGAKDLIEHLLIVEKKKRFTAIDTLSHPWIMYNGDMGKITDNAELEKLKTKTRNELE 835 HSP 2 Score: 140.198 bits (352), Expect = 5.004e-30 Identity = 102/307 (33.22%), Postives = 156/307 (50.81%), Query Frame = 3
Query: 153 SQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYF--SNFIPTGVRRIYDRNGGQ-VQDIDDLEHRNIYICCGQESLK-TYDYDTKYFE--LIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKS----------KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAEL-FPN 1022
SQ+ +DI D ++LR+P+++ F VNG+ F GK L+ITP+RY NDLL+DLT S +P GVR+IY +GG+ V +I+ L IY+ G E+ K Y + E + G N N+ P + D + YRG R Y + R Y N S KP+++TI+R G +P + KILLN+ S QS+E L+ D+S+AFG ++ ++++Y I G+E+Q SDFFR FVA+G + L+ ++ +I+ E+ FP+
Sbjct: 18 SQTHKDIVDTKMKDLRRPRKIRFYVNGDGYFKGKKLNITPHRYSTFNDLLNDLTGKLPSSVNLPYGVRQIYTPSGGRRVTEIEGLADGQIYVLAGFEAFKPQVRYGQQVLEPWSMEVGR-RNLNAR------PHNHDIEGDGAMYGHGS--YRGGRSYGAFQPGPYLQKSTG----------HRRYPGAFGTANKGSYLQNADSGQVKPKVITIVRNGARPRNNIKILLNRRSVQSFEQLMLDVSEAFGPKYRNNKMEKLYTIRGREVQGISDFFRDENVFVAVGIE--KLSGGDLIDIIEEIDFPD 303
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Match: F6SUL1 (Protein kinase domain-containing protein OS=Ciona intestinalis OX=7719 GN=LOC100181877 PE=3 SV=2) HSP 1 Score: 346.665 bits (888), Expect = 8.748e-107 Identity = 158/268 (58.96%), Postives = 207/268 (77.24%), Query Frame = 3
Query: 2370 DFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGN 3170
+ E Y++G +IG GNF+ VKE + + T FA KIIDK +LK KED+IE EI I+K+C HPNIV+L E+FET N+++LV EY+K GDLFDAI E++KF E +A M+ D+ AL ++HSK I+HRD+KPENLL S K+G + LKL+DFGLA+E+ EP+Y +CGTPTYVAPEIL+E GYG+EVD+WA+GVI YILLCGFPPFRS ER+Q ELFE I+ G++E++ PYWD IS +KDLI +L+VD +R+T L HPWI + G
Sbjct: 5 NIEARYEIGKTIGDGNFAVVKECRLRNTESEFAMKIIDKSKLKGKEDMIENEIAIMKNCHHPNIVRLIEEFETENEIYLVLEYVKGGDLFDAITESVKFTERDAANMVADLSEALAFLHSKNIIHRDLKPENLLVSRNKDGSMTLKLADFGLAMEVTEPIYTVCGTPTYVAPEILAETGYGLEVDMWATGVITYILLCGFPPFRSLERDQEELFEIIQLGDYEFLSPYWDNISEAAKDLIQRLLVVDTKRRYTAEQVLSHPWIQSEGT 272
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Match: R7TC90 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_217544 PE=4 SV=1) HSP 1 Score: 359.377 bits (921), Expect = 3.104e-105 Identity = 161/279 (57.71%), Postives = 216/279 (77.42%), Query Frame = 3
Query: 2343 LAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSI 3176
L +Q+ + + Y + ++G GNF+ VK+ + K T +A KIIDK +LK KE +IE EI I+K C NIV+L E+FET + ++L+ E +K GDLFDAI +++KFPE +S+ M+RD+ AL Y+HS+ IVHRD+KPENL+ C K+G ++LKL+DFGLA+E+K+ ++ +CGTPTYVAPEILSE GYG+EVD+WA GVI YILLCGFPPFRSP+RNQTELFEFIKAGE+E++ PYWD+IS +KDLI+ +L+VD KRFT +D L H WIIT G S+
Sbjct: 489 LERQVSSVQHVMDRYDVSKTLGDGNFAIVKQCQLKETKADYAMKIIDKAKLKGKEQMIENEIEIMKKCNQANIVRLIEEFETKDDIYLIMELVKGGDLFDAITQSVKFPEVKSSSMVRDLANALHYLHSREIVHRDLKPENLMVCHNKDGSMSLKLADFGLAMEVKQDIFTVCGTPTYVAPEILSETGYGLEVDMWALGVISYILLCGFPPFRSPDRNQTELFEFIKAGEYEFLSPYWDKISRSAKDLISRLLVVDRKKRFTAIDTLSHAWIITNGGSL 767 HSP 2 Score: 93.2041 bits (230), Expect = 2.258e-15 Identity = 41/105 (39.05%), Postives = 69/105 (65.71%), Query Frame = 3
Query: 708 ETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPN 1022
+ N+ KP+++T++R KP + KILLN+ S QSYE L+ DI++AFG WK +++++ + G+E++ SDFFR F+ IG++ L+ V I+ EL+P+
Sbjct: 139 QENIPMKPKVITVVRNSGKPRTSVKILLNRRSVQSYEQLMKDITEAFGPKWKNNKMRKLFTVRGREVKGISDFFREDDVFIGIGNE--QLSSGHVAEILEELYPD 241
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Match: A0A1I8IN06 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 339.732 bits (870), Expect = 5.824e-104 Identity = 150/270 (55.56%), Postives = 205/270 (75.93%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIR 3179
FE+Y LG ++G GNF+ V+ K+ +G +AAK+IDKK+L+ KE ++ E+RI++ C HPNIV+L +++ET +++L+ E +K GDLFDAI + +KF E Q++ M+ DI AL Y+HS+ IVHRD+KPENLL K+ I+LKL DFGLA+E++E ++ +CGTPTYVAPEILSE GYG+EVD+WA GVI YILLCGFPPFRS +R Q+ELF+ IK G FEY+ PYWD +S +KDLIN +L+VD R T +D LCH W+++ G S R
Sbjct: 2 FERY-DLGRTLGDGNFAVVRACVKRDSGNEYAAKVIDKKKLRGKEHMVLNEVRIMRLCRHPNIVRLVDEYETKAEIYLIMELVKGGDLFDAISQVVKFNEIQASSMVADIAGALHYLHSRRIVHRDLKPENLLVYRSKDNKISLKLGDFGLAMEVEEAIFTVCGTPTYVAPEILSETGYGLEVDMWALGVIAYILLCGFPPFRSRDRRQSELFQMIKTGTFEYLSPYWDHVSAPAKDLINRLLVVDKAARLTAIDVLCHAWVLSRGGSQR 270
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Match: ENSAMXT00000039497.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:8818577:8831816:1 gene:ENSAMXG00000040052.1 transcript:ENSAMXT00000039497.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 298.901 bits (764), Expect = 1.060e-87 Identity = 140/268 (52.24%), Postives = 187/268 (69.78%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWII---TLGNSI 3176
Y++G IG GNF+ VKE ++ATG+ +A KIIDK + KE LI E+ IL+ HP+I+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++ M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q ELFE I G E+ PYWD ++P +KDLI ML V+ R+T + L HPW++ TL N++
Sbjct: 384 YKVGKVIGDGNFAVVKECVERATGQEYALKIIDKAKCSGKEHLIANEVAILRRVRHPSIIMLIEEVDTPTELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLTGALRYLHRMCIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSERNQQEELFEQILLGRLEFPAPYWDNVTPSAKDLIGKMLQVNVSARYTAEEVLSHPWVLDDSTLENNV 651 HSP 2 Score: 68.9366 bits (167), Expect = 2.490e-11 Identity = 28/78 (35.90%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
KP+++T+IR+G+KP +ILLNK ++ S++ +L+DI++A +++++Y +DGK++ DFF F+A G
Sbjct: 195 KPKLVTVIRSGVKPRKAVRILLNKKTAHSFDQVLADITEAI--KLDSGAVKKLYTLDGKQLTSLQDFFGDDDVFIACG 270 HSP 3 Score: 58.9214 bits (141), Expect = 2.400e-8 Identity = 32/104 (30.77%), Postives = 60/104 (57.69%), Query Frame = 3
Query: 153 SQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
+++ R +T + ++ ++V F NG+R F+G L +++ +R+ L+ LL +LT+ ++ +P GVR IY +G ++ +D+L Y+C ES K DY
Sbjct: 62 TRTLRALTSE-----KRARKVRFYRNGDRYFTGLLYAVSADRFRSLDTLLAELTRSLADSLHLPQGVRAIYTVDGAKKISSLDELVEGESYVCASNESYKKVDY 160
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Match: ENSAMXT00000043405.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:8818577:8831816:1 gene:ENSAMXG00000040052.1 transcript:ENSAMXT00000043405.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 298.516 bits (763), Expect = 1.132e-87 Identity = 140/268 (52.24%), Postives = 187/268 (69.78%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWII---TLGNSI 3176
Y++G IG GNF+ VKE ++ATG+ +A KIIDK + KE LI E+ IL+ HP+I+ L E+ +TP +++LV E +K GDLFDAI + K+ E ++ M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q ELFE I G E+ PYWD ++P +KDLI ML V+ R+T + L HPW++ TL N++
Sbjct: 370 YKVGKVIGDGNFAVVKECVERATGQEYALKIIDKAKCSGKEHLIANEVAILRRVRHPSIIMLIEEVDTPTELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLTGALRYLHRMCIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSERNQQEELFEQILLGRLEFPAPYWDNVTPSAKDLIGKMLQVNVSARYTAEEVLSHPWVLDDSTLENNV 637 HSP 2 Score: 68.5514 bits (166), Expect = 2.584e-11 Identity = 28/78 (35.90%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
KP+++T+IR+G+KP +ILLNK ++ S++ +L+DI++A +++++Y +DGK++ DFF F+A G
Sbjct: 195 KPKLVTVIRSGVKPRKAVRILLNKKTAHSFDQVLADITEAI--KLDSGAVKKLYTLDGKQLTSLQDFFGDDDVFIACG 270 HSP 3 Score: 58.9214 bits (141), Expect = 2.609e-8 Identity = 32/104 (30.77%), Postives = 60/104 (57.69%), Query Frame = 3
Query: 153 SQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
+++ R +T + ++ ++V F NG+R F+G L +++ +R+ L+ LL +LT+ ++ +P GVR IY +G ++ +D+L Y+C ES K DY
Sbjct: 62 TRTLRALTSE-----KRARKVRFYRNGDRYFTGLLYAVSADRFRSLDTLLAELTRSLADSLHLPQGVRAIYTVDGAKKISSLDELVEGESYVCASNESYKKVDY 160
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 302.753 bits (774), Expect = 1.227e-87 Identity = 147/320 (45.94%), Postives = 208/320 (65.00%), Query Frame = 3
Query: 2361 TDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWII---TLGNSIR----------FSKPIKDVVTETRVELQMETAIFKSNNTISKNT 3278
T + Y++G IG GNF+ VK+ +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP++++LV E +K GDLFD+I + K+ E ++ M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G ++ PYW+ IS +K+LI ML V+ R+T D LCHPW+ + N+++ F+ K T V + M TA+ K N +S++
Sbjct: 434 TSSSILEKYKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWVTEDAAMENNMKMEVTGKLKKHFTSVQKQGNTSAGVSVIMSTALDKENIQLSRHA 753 HSP 2 Score: 72.4034 bits (176), Expect = 1.996e-12 Identity = 51/174 (29.31%), Postives = 86/174 (49.43%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSPDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNK 1247
KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA ++R+Y ++GK+I DFF F+A G + Q++ + + K S+ + R++ K+ + + MT R SP ++ R + S V P H ST Q P K
Sbjct: 189 KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGVVKRLYTLEGKQITCLQDFFGEDDVFIACGPEKFRYAQDDF------VLDHSGKASEAFLTECRVL---KSSYGSRSMTPVRAGGS---KSPGSTRR------SKSPGTVRRNPGHFSTSSQSPVK 342 HSP 3 Score: 56.6102 bits (135), Expect = 1.634e-7 Identity = 32/112 (28.57%), Postives = 62/112 (55.36%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
S++ + +++ + +T + +K ++V F NG+R F G + +++ +R+ L+ LL +LT+ S+ + P GVR IY +G +V +DDL Y+C E + +Y
Sbjct: 43 SAHCSFYRTRTLQSLTTE-----KKARKVRFYRNGDRYFKGLVYAVSGDRFRSLDALLMELTRSLSDNVNLPQGVRFIYTVDGARRVTSLDDLLEGESYVCASNEPFRKMEY 149
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 299.671 bits (766), Expect = 1.231e-87 Identity = 138/270 (51.11%), Postives = 190/270 (70.37%), Query Frame = 3
Query: 2349 QQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
+ L + EKY ++G IG GNF+ VK+ +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP++++LV E +K GDLFD+I + K+ E ++ M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G ++ PYW+ IS +K+LI ML V+ R+T D LCHPW+
Sbjct: 415 KNLTSSSILEKY-KVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWV 683 HSP 2 Score: 72.4034 bits (176), Expect = 1.929e-12 Identity = 51/174 (29.31%), Postives = 86/174 (49.43%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSPDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNK 1247
KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA ++R+Y ++GK+I DFF F+A G + Q++ + + K S+ + R++ K+ + + MT R SP ++ R + S V P H ST Q P K
Sbjct: 189 KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGVVKRLYTLEGKQITCLQDFFGEDDVFIACGPEKFRYAQDDF------VLDHSGKASEAFLTECRVL---KSSYGSRSMTPVRAGGS---KSPGSTRR------SKSPGTVRRNPGHFSTSSQSPVK 342 HSP 3 Score: 56.225 bits (134), Expect = 2.020e-7 Identity = 32/112 (28.57%), Postives = 62/112 (55.36%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
S++ + +++ + +T + +K ++V F NG+R F G + +++ +R+ L+ LL +LT+ S+ + P GVR IY +G +V +DDL Y+C E + +Y
Sbjct: 43 SAHCSFYRTRTLQSLTTE-----KKARKVRFYRNGDRYFKGLVYAVSGDRFRSLDALLMELTRSLSDNVNLPQGVRFIYTVDGARRVTSLDDLLEGESYVCASNEPFRKMEY 149
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338]) HSP 1 Score: 298.901 bits (764), Expect = 1.720e-87 Identity = 135/258 (52.33%), Postives = 185/258 (71.71%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VK+ +++TGK FA KIIDK + + KE LIE E+ +L+ +HPNI+ L E+ +TP++++LV E +K GDLFD+I + K+ E ++ M+ ++ AL Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PLY +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q +LF+ I G ++ PYW+ IS +K+LI ML V+ R+T D LCHPW+
Sbjct: 378 YKVGKIIGDGNFAVVKDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATLVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNQQEDLFDQILQGRLDFPSPYWENISHSAKELIGRMLQVNVEARYTAEDVLCHPWV 635 HSP 2 Score: 71.2478 bits (173), Expect = 3.832e-12 Identity = 31/78 (39.74%), Postives = 51/78 (65.38%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
KP+++T+IR+G+KP +ILLNK ++ S+E +L+DI+DA ++R+Y ++GK+I DFF F+A G
Sbjct: 190 KPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAI--KLDSGVVKRLYTLEGKQITCLQDFFGEDDVFIACG 265 HSP 3 Score: 54.299 bits (129), Expect = 8.172e-7 Identity = 32/112 (28.57%), Postives = 62/112 (55.36%), Query Frame = 3
Query: 129 SSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
S++ + +++ + +T + +K ++V F NG+R F G + +++ +R+ L+ LL +LT+ S+ + P GVR IY +G +V +DDL Y+C E + +Y
Sbjct: 44 SAHCSFYRTRTLQSLTTE-----KKARKVRFYRNGDRYFKGLVYAVSGDRFRSLDALLMELTRSLSDNVNLPQGVRFIYTVDGARRVTSLDDLLEGESYVCASNEPFRKMEY 150
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010548.1 (pep scaffold:Pmarinus_7.0:GL477749:266235:284843:-1 gene:ENSPMAG00000009555.1 transcript:ENSPMAT00000010548.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 305.834 bits (782), Expect = 3.121e-91 Identity = 141/290 (48.62%), Postives = 200/290 (68.97%), Query Frame = 3
Query: 2337 PVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKP--------FAAKIIDKKRLKDKEDLIEEEIRIL-KSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSI-KNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEIS---PESKDLINNMLIVDPLKRFTVLDCLCHPWIITLG 3167
P L + + +D E Y++G +IG GNF+ VKE ++ ++G A KIIDK +L +E+++ E+ + + +HPN+V+L E E+P+ +LV E ++ GDLFDAI E+++F E +A M+ D+ RAL Y+H+ IVHRD+KPENLL +G LKL+D GLA+E+ P+Y +CGTPTYVAPEIL+E GYG+EVD+WA+GVI YILLCGFPPFRS ER+Q ELFE I+ GE+E++ PYWD IS +KDL++ +L+VD +R+T L HPW+ G
Sbjct: 347 PNLPMPIVSLQDIESRYEIGRTIGDGNFATVKECRRVSSGDGSAGEAEWDLAMKIIDKAKLVGREEMLRSEVALTTRLGQHPNVVRLVEAMESPSDAYLVLERVRGGDLFDAITESVRFTERHAAAMVADLCRALQYLHANNIVHRDVKPENLLVQRNADGGTTLKLADLGLAMEVTGPIYTVCGTPTYVAPEILAETGYGLEVDMWAAGVITYILLCGFPPFRSLERDQEELFEIIQLGEYEFLSPYWDHISDGHEAAKDLVSRLLVVDTARRYTARQALHHPWVSDGG 636 HSP 2 Score: 88.1965 bits (217), Expect = 4.361e-18 Identity = 76/261 (29.12%), Postives = 118/261 (45.21%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFIPTGVRRIYDRNGGQ--VQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSF-FKILLNKTSSQSYEDLLSDISDAFG-SNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKD 968
R+PK V F NG+R F + L ITP+RY +LL DL++ +P GVRR+Y GG +D L Y+C E ++ K F + N + YN +T T +G R N L +P E + ++P+++T++R G + +LLN+ ++QS+E+LL DIS+A G W+ ++R+Y + G+E + SDFFR F+A KD
Sbjct: 1 RRPKVVRFYRNGDRYFRARTLHITPHRYVSWPELLADLSRAVP--LPYGVRRLYTPLGGSPVAGSVDALRDGEAYVCASFEPFRS----VKDF---------SRNKQPQLSYN---------ATCGASTICLDKGVR---------------------QNFLR----RP---ENYVPARPKVITVVRVGGRGPRRRITLLLNRRAAQSFEELLGDISEALGLPRWRTNHVRRLYTLRGREARSVSDFFRGDDVFLAATRKD 209
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008748.1 (pep scaffold:Pmarinus_7.0:GL476577:591:63029:-1 gene:ENSPMAG00000007907.1 transcript:ENSPMAT00000008748.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 216.468 bits (550), Expect = 1.267e-62 Identity = 109/265 (41.13%), Postives = 165/265 (62.26%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDL-IEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEI---KEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
F YQL +G G FS V+ K +TG+ FAAKII+ K+L ++ +E E RI + +H NIV+L E +LV + + G+LF+ I + E+ ++ ++ I ++ + H GIVHRD+KPENLL + K +KL+DFGLA+E+ ++ + GTP Y++PE+L + YG VD+WA GVI YILL G+PPF + +Q +L++ IKAG +++ P WD ++ E+K+LIN ML ++P KR T + L HPWI
Sbjct: 10 FTDEYQLYEELGKGAFSVVRRCVKVSTGQEFAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHESIAEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVHHCHQHGIVHRDLKPENLLLASKMKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFW--DEDQHKLYQQIKAGAYDFPSPEWDTVTAEAKNLINQMLTINPAKRITAAEALKHPWI 272
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003135.1 (pep scaffold:Pmarinus_7.0:GL491838:799:5626:1 gene:ENSPMAG00000002863.1 transcript:ENSPMAT00000003135.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 197.593 bits (501), Expect = 2.495e-57 Identity = 103/257 (40.08%), Postives = 161/257 (62.65%), Query Frame = 3
Query: 2412 GNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEY----IKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVI---CGTPTYV---APEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPW 3152
G FSEV A++++TG+ A K I K L+ K++ +E E+ +L +H NI+ LE+ +ET + ++L+ + I+ G+LFD I E + E ++ ++R + RA+ ++H +G+VHRD+KPENLL + +SDFGL+ +++ P V+ CGTP YV APE+L+++ Y VD W+ GVI YILLCG+PPF + + LF+ I E+E+ PYWD+IS +KD I +++ D +RFT L HPW
Sbjct: 2 GAFSEVVLAEERSTGRHVAIKCIAKTALRGKDNTVENEVCVLSRIKHENIIALEDIYETDSHLYLIMQLVLLLIRGGELFDRIVERGFYTERDASALIRQVLRAVSHLHGQGVVHRDLKPENLLYYSAEDDSKIMISDFGLS-KVEAPGSVMSTACGTPGYVACEAPEVLAQKPYTRAVDCWSIGVISYILLCGYPPFY--DEVDSRLFDQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDVAERFTCEQALQHPW 255
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Match: phkg1b (phosphorylase kinase, gamma 1b (muscle) [Source:ZFIN;Acc:ZDB-GENE-050522-52]) HSP 1 Score: 201.06 bits (510), Expect = 1.017e-56 Identity = 114/289 (39.45%), Postives = 168/289 (58.13%), Query Frame = 3
Query: 2337 PVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIID-------KKRLKDKEDLIEEEIRILKSCE-HPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIK--EPLYVICGTPTYVAPEIL------SEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
PV AQ DF Y+ +GSG S V+ K TG+ FA KIID ++ L+D +E+ ILK+ HP+I+ L + +E+ FLV + +K G+LFD + E + E + +MR IF + ++H+K IVHRD+KPEN+L + +N++LSDFG +++++ E L +CGTP Y++PE+L S GYG EVD+WA GVI Y LL G PPF R Q + I G++++ P W++ S KDLI+ +L+V+P KRFT L HP+
Sbjct: 15 PVAAQ------DFNDKYEPKEVLGSGVSSVVRRCVHKRTGQDFAVKIIDITPDRLSEEELEDIYSSTVKEVDILKAVSGHPHIITLIDAYESSTFFFLVFDLMKRGELFDYLTEKVALSEKDTRSIMRSIFEVVHFLHAKNIVHRDLKPENILL---DDNMNIRLSDFGFSMQLRPGERLRELCGTPGYLSPELLKCSMDESHPGYGKEVDLWACGVIMYTLLAGSPPFW--HRKQMTMLRLIMDGKYDFSTPEWEDRSDTVKDLISRLLVVEPEKRFTAEQALAHPFF 292
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Match: stk33 (serine/threonine kinase 33 [Source:ZFIN;Acc:ZDB-GENE-040724-242]) HSP 1 Score: 194.126 bits (492), Expect = 1.604e-55 Identity = 103/286 (36.01%), Postives = 165/286 (57.69%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE-DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK---NGL--INLKLSDFGLAVEIKEP------LYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPI 3194
F+ YY+LG +GSG+F V EA TG +A K ++K+R L+E E+ ILK +H NI+ LEE ET +V+LV E + G+L + + + E+++ + + A+ Y+H IVHRD+K EN+L G+ ++++++DFGL+ ++E L CGTP Y+A E++ Q Y + D+W+ GVI Y+LLCG PPF + +Q +LFE I GE + +P W+E+S +K+L+ +L VDP R T + L W+ G++ +P+
Sbjct: 1 FQNYYRLGRQLGSGSFGTVYEATHVETGVKWAIKTVNKERAGSSGVRLLEREVVILKRVQHQNIIHLEEVLETHKRVYLVMELCELGELRAVLEQRKRLSEAEARHVAHSLAGAIAYLHKHDIVHRDLKLENILAKSSEPHEGIDHLDIRVTDFGLSA-VREGNGAASMLQSPCGTPIYMAAEVIQSQDYSQQCDVWSIGVILYMLLCGEPPFMA--HSQEKLFELIIKGELSFTQPAWEEVSESAKELLRGLLKVDPAHRLTASELLHDHWVT--GDTSSLGRPL 281
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Match: CMK1 (Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication [Source:SGD;Acc:S000001910]) HSP 1 Score: 199.904 bits (507), Expect = 4.450e-56 Identity = 110/269 (40.89%), Postives = 165/269 (61.34%), Query Frame = 3
Query: 2376 EKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIE---EEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIK---EPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEF--EYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWII 3158
+K Y G ++G+G F V++AK TG+ A KI+ KK LK + +E +E+ IL+ HPNIV ++ FE+ +K +++ + K G+LFD I + KF E + ++ +I A+ Y+HS+ IVHRD+KPENLL K+ L ++DFG+A +K E LY G+ YVAPE+L++ G+G DIW+ GVI Y LLCG+ FR+ ER Q + + GE+ ++ +PYWD +S ++K I L +DP KR T + L PWII
Sbjct: 34 KKKYVFGKTLGAGTFGVVRQAKNTETGEDVAVKILIKKALKGNKVQLEALYDELDILQRLHHPNIVAFKDWFESKDKFYIITQLAKGGELFDRILKKGKFTEEDAVRILVEILSAVKYMHSQNIVHRDLKPENLLYIDKSDESPLVVADFGIAKRLKSDEELLYKPAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSAFRA-ERVQ-DFLDECTTGEYPVKFHRPYWDSVSNKAKQFILKALNLDPSKRPTAAELLEDPWII 300
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Match: DUN1 (Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role [Source:SGD;Acc:S000002259]) HSP 1 Score: 199.134 bits (505), Expect = 2.990e-55 Identity = 118/333 (35.44%), Postives = 181/333 (54.35%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKD--KEDLIEEEIRILKSCEHPNIVKLEEQFETPN-----KVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGL----------------INLKLSDFGLAVEIKEPLYV--ICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS*L*CL 3296
F+KY LG +G+G+++ VKEAK K TG+ A KI ++ D K EE IL +HPNIV L + F P + +LV E I +G+LF+ I + +S + + + L Y+H + I+HRDIKPEN+L +I I +K++DFGLA E + +CGTP+YVAPE+L+++GY +VD+W++GVI Y+ LCGFPPF S + L E I ++ + PYWD+I LI+N+L+++P +R+ + + L HPW D+ ++ V L+++ ++N I K S L CL
Sbjct: 197 FDKYL-LGKELGAGHYALVKEAKNKKTGQQVAVKIFHAQQNDDQKKNKQFREETNILMRVQHPNIVNLLDSFVEPISKSQIQKYLVLEKIDDGELFERIVRKTCLRQDESKALFKQLLTGLKYLHEQNIIHRDIKPENILLNITRRENPSQVQLGPWDEDEIDIQVKIADFGLAKFTGEMQFTNTLCGTPSYVAPEVLTKKGYTSKVDLWSAGVILYVCLCGFPPF-SDQLGPPSLKEQILQAKYAFYSPYWDKIDDSVLHLISNLLVLNPDERYNIDEALNHPW-------------FNDIQQQSSVSLELQRLQI-TDNKIPKTYSELSCL 513
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Match: CMK2 (Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005376]) HSP 1 Score: 180.259 bits (456), Expect = 2.406e-49 Identity = 109/306 (35.62%), Postives = 169/306 (55.23%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE---DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIK---EPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEF--EYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVT--ETRVELQMETAIFKSNNTISK 3272
Y G ++G+G+F V++A+K +T + A KI+ KK L+ ++ EE+ IL+ HPNIV ++ FE+ +K ++V + G+LFD I KF E + ++ I A+ Y+HSK +VHRD+KPEN+L K+ L ++DFG+A ++K + +Y G+ YVAPE+L++ G+G DIW+ GVI Y LLCG+ PF + + E A + + PYWD IS + K I L ++P R T + L PWI + + S + DV R +L+ I K NN I +
Sbjct: 47 YIFGRTLGAGSFGVVRQARKLSTNEDVAIKILLKKALQGNNVQLQMLYEELSILQKLSHPNIVSFKDWFESKDKFYIVTQLATGGELFDRILSRGKFTEVDAVEIIVQILGAVEYMHSKNVVHRDLKPENVLYVDKSENSPLVIADFGIAKQLKGEEDLIYKAAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSPFIA--ESVEGFMEECTASRYPVTFHMPYWDNISIDVKRFILKALRLNPADRPTATELLDDPWITS--KRVETSNILPDVKKGFSLRKKLRDAIEIVKLNNRIKR 348
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Match: KCC4 (Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication [Source:SGD;Acc:S000000529]) HSP 1 Score: 179.489 bits (454), Expect = 2.074e-46 Identity = 105/289 (36.33%), Postives = 164/289 (56.75%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDL----------IEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEP--LYVICGTPTYVAPEILSEQGY-GIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTE 3212
++LG ++G G+ +V+ A+ + TG A K+I K + + IE EI I+K HPN++ L + +ET N ++L+ EY ++G+LF+ + ++ PE ++ R I + Y H+ GIVHRD+KPENLL + N+K++DFG+A + L CG+P Y APEI+S Y G D+W+ GVI + LL G PF N +L ++ G+FE P EIS +++DLI +L+VDP +R + D L HP ++ +I+ SK IKD+ E
Sbjct: 21 WKLGETLGFGSTGKVQLAQHERTGHRTAVKVISKSIFNNNGNHSNDDSVLPYNIEREIVIMKLLSHPNVLSLYDVWETNNNLYLILEYAEKGELFNLLVDHGPLPEREAINCFRQIIIGISYCHALGIVHRDLKPENLLL---DSFYNIKIADFGMAALQTDADLLETSCGSPHYAAPEIVSGLPYEGFASDVWSCGVILFALLTGRLPFDEENGNVRDLLLKVQKGQFEM--PNDTEISRDAQDLIGKILVVDPRQRIKIRDILSHP-LLKKYQTIKDSKSIKDLPRE 303
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Match: GIN4 (Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication [Source:SGD;Acc:S000002915]) HSP 1 Score: 176.792 bits (447), Expect = 2.030e-45 Identity = 112/295 (37.97%), Postives = 161/295 (54.58%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE------------DL----IEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEP--LYVICGTPTYVAPEILSEQGY-GIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTE 3212
++LG ++G G+ +V+ A+ +TG+ A K+I K D IE EI I+K HPN+++L + +ET ++LV EY ++G+LF+ + E PE ++ R I + Y H+ GIVHRD+KPENLL K N+K++DFG+A E L CG+P Y APEI+S Y G D+W+ GVI + LL G PF + N L ++ GEFE P DEIS E++DLI +L VDP +R D L HP ++ SIR SK I+ + E
Sbjct: 19 WKLGETLGLGSTGKVQLARNGSTGQEAAVKVISKAVFNTGNVSGTSIVGSTTPDALPYGIEREIIIMKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVERGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHK---YNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLLKVQKGEFEM--PSDDEISREAQDLIRKILTVDPERRIKTRDILKHP-LLQKYPSIRDSKSIRGLPRE 307
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Match: EDO42432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1C2]) HSP 1 Score: 324.709 bits (831), Expect = 8.644e-100 Identity = 144/273 (52.75%), Postives = 200/273 (73.26%), Query Frame = 3
Query: 2337 PVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK-NGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPW 3152
P + L TD++ Y G +G GNF+ V++ K K T K FA KIIDK++++ KE +I++EI I++ C HPNIV+L E +++ +++LV E IK GDLFDAI ++KF E + RD+ +AL Y+H + IVHRD+KPENLL + +G +LKL+DFGLA+E+ P++ +CGTPTYVAPEIL E GYG++VD+WA+GVI YI+LCGFPPFRSP R+Q ELF+ I++GEFE++ PYWD +S ++KDLI+ ML+VDP KR T L H W
Sbjct: 230 PKYKRPLVTDKEVTDVYNFGQKLGDGNFAIVRQCKDKITQKEFAIKIIDKRKIRGKEKMIDDEIAIMRRCRHPNIVRLFEDYDSATEIYLVMELIKGGDLFDAISSSVKFTEHVAKSYFRDMCKALAYLHKRKIVHRDLKPENLLVHKRSDGQTHLKLADFGLAMEVTAPIFTVCGTPTYVAPEILEENGYGLKVDMWAAGVITYIMLCGFPPFRSPNRDQDELFDLIQSGEFEFLSPYWDPVSDDAKDLISTMLVVDPTKRATADQVLQHRW 502 HSP 2 Score: 51.2174 bits (121), Expect = 2.146e-6 Identity = 32/87 (36.78%), Postives = 48/87 (55.17%), Query Frame = 3
Query: 207 KRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFIPTGVRRIYDR-NGGQVQDIDDLEHRNIYICCGQESLKTYDYDTK 464
K V+F NG+ FS ++ITP +Y + LL +L+ +P GVR + D+ NG QV++I+DL+ Y+C LK Y K
Sbjct: 6 KTVIFYKNGD-VFSKYPVTITPRKYRSFDILLAELS--MKTELPQGVRYLLDKENGAQVKNIEDLQDGKEYVCASNARLKRLAYGKK 89
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Match: EDO44488 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVP7]) HSP 1 Score: 313.153 bits (801), Expect = 6.444e-99 Identity = 145/258 (56.20%), Postives = 198/258 (76.74%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ V+E K + T K +A KII+K ++K KE +IE EI IL+ +H +IV+L E++ETP ++FLV E +K GDLF+AI E K+ E ++ M+RD+ AL Y+H IVHRDIKPENLL ++ NG +LKL+DFGLA+E+K P++++CGTPTYVAPEIL E GYG++VDIWA+GVI YILLCGFPPFRSP +Q ELF+ I AGEFEY+ P+W++IS +KDLI++ML+VD +R++ L HPW+
Sbjct: 1 YRIGKVIGDGNFAVVRECKHRKTNKEYALKIINKAKVKGKEHMIENEISILRRVKHNHIVELIEEYETPREIFLVMELVKGGDLFEAIVEATKYTEVHASHMVRDLASALDYLHCNSIVHRDIKPENLLVLNLPNGRKSLKLADFGLAMEVKSPMFLVCGTPTYVAPEILDESGYGLKVDIWAAGVISYILLCGFPPFRSP--DQDELFDLILAGEFEYLSPFWNDISSSAKDLIDHMLVVDANQRYSAKQVLAHPWV 256
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Match: EDO36253 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ85]) HSP 1 Score: 230.72 bits (587), Expect = 4.198e-68 Identity = 120/266 (45.11%), Postives = 166/266 (62.41%), Query Frame = 3
Query: 2364 DEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLA-VEIKEPLY-VICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
D+ K Y L +G+G FSEV A++KATG FA K I KK LK KE+ +E EI ILK H NI+ L++ F+ + ++LV + ++ G+LFD I E + E ++ +++ I A Y+HS GIVHRD+KPENLL + + +SDFGL+ +E + CGTP YVAPE+L +Q YG VD W+ GVI YILLCG+PPF + + LF I E+E+ PYWDEIS +KD I +++ +DP R+T L HPWI
Sbjct: 10 DDKITKKYDLREVLGTGAFSEVVLAEEKATGSLFAVKCISKKELKGKEESVENEIAILKKVRHKNIIGLKDIFDNKSHLYLVMDLVQGGELFDRIVEKGNYTEQDASALVQQILEAADYLHSLGIVHRDLKPENLLYYSPDEDSKIMISDFGLSKIEAQGSFMDTACGTPGYVAPEVLKQQPYGKAVDCWSIGVITYILLCGYPPFY--DDSDANLFAQIMRAEYEFDSPYWDEISESAKDFIRHLMELDPKLRYTCRQALSHPWI 273
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Match: EDO49898 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF52]) HSP 1 Score: 208.379 bits (529), Expect = 2.875e-58 Identity = 104/266 (39.10%), Postives = 159/266 (59.77%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKE-DLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEI---KEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWII 3158
F + Y+L +G G FS V++ + T +A KI+D K + ++ +E E RI + HPN+V+L + FLV + + G+LF+ I + E+ ++ ++ + ++ + H GIVHRD+KPENLL + + +KL+DFGLA+E+ + Y GTP Y++PE+L + YG VD+WA GVI YILL G+PPF E Q +L+ IKAG ++Y P WD ++ ++KDLI+ ML VD KR T + L HPWI+
Sbjct: 13 FNEKYELKEELGKGAFSTVRKCCHRETKIEYAVKILDTKNMTQRDVHKVEREARICRHLRHPNVVRLHANIMSDGFYFLVFDLVTGGELFEDIVAREYYSEADASHCIQQVLLSVQHCHENGIVHRDLKPENLLLASRERGAMVKLADFGLAIEVDGERLGWYGFAGTPGYLSPEVLKKDPYGKPVDLWACGVILYILLVGYPPFWDEE--QQKLYSQIKAGTYDYPSPEWDTVTADAKDLIDKMLTVDAPKRITAAEALKHPWIV 276
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Match: EDO44594 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV77]) HSP 1 Score: 205.297 bits (521), Expect = 3.241e-58 Identity = 118/278 (42.45%), Postives = 164/278 (58.99%), Query Frame = 3
Query: 2358 ETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLI---EEEIRILKSCE-HPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIK--EPLYVICGTPTYVAPEILS------EQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
E F Y+ +G G S V++ K TGK FA KIIDK + D+I +E+ IL S + H NI++LE+ FE+ FLV E +G+LFD + E + E Q+ +M IF A+ Y+H +VHRD+KPEN+L + IN+K+SDFG AVE+K E L +CGTP Y+APE+L GY EVD+WA GVI Y LLCGFPPF R Q + I G++ + P W+++S ++K+LI +L+V P +R T D L HPW+
Sbjct: 14 EATGGFIDKYEPKELLGCGVSSIVRKCYDKLTGKAFAVKIIDKLSDQGGSDIIATTRDEVNILLSLQGHKNIIRLEDVFESTAFFFLVFEIAPKGELFDYLTEKVTLSEKQTRRIMLSIFEAVDYMHYHNVVHRDLKPENILLDEE---INVKISDFGFAVELKEGETLRELCGTPGYLAPEVLQCSMFPDAPGYTKEVDMWACGVILYTLLCGFPPFW--HRRQVVMLRNIMNGKYNFSSPEWEDVSNDAKNLIKKLLVVHPKERITASDALKHPWL 286
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Match: dclk3 (doublecortin like kinase 3 [Source:NCBI gene;Acc:101156024]) HSP 1 Score: 285.034 bits (728), Expect = 2.219e-85 Identity = 131/281 (46.62%), Postives = 184/281 (65.48%), Query Frame = 3
Query: 2361 TDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCS-IKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKD 3200
T D E+ Y+ G IG GNF+ V+E +++ G+ A KI+++ +L +E +++ E+ +L S HP IV+L T +LV E + GDLF+AI + KFPE+++ +++ D+ AL YIH K IVHRD+KPENLL + + LKL DFGLA+ + EP++ ICGTPTYVAPEIL E GYG+ VD+WA GVI ++LLCGFPPFRS ER+Q ELF+ IK + + PYWD IS E+K L+ +L VDP R T L HPW+ + S+ + + D
Sbjct: 67 TLSDIERVYEFGRVIGDGNFAVVRECRRRDGGELLALKIVERSKLVGREHMMQNELSLLGSLCHPRIVRLFTHHHTRTHSYLVMELVSGGDLFEAISDRGKFPEAEAGLIVSDVSEALEYIHRKSIVHRDLKPENLLVERVAASVCRLKLGDFGLAMVVTEPVFTICGTPTYVAPEILHETGYGVAVDVWALGVILFVLLCGFPPFRSRERDQEELFKLIKQAQLHFPSPYWDSISEEAKSLVRCLLEVDPTLRLTAEQTLQHPWVKAMA-SVCMQRALTD 346
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Match: ENSORLT00000003850.2 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659]) HSP 1 Score: 293.508 bits (750), Expect = 2.501e-85 Identity = 138/258 (53.49%), Postives = 182/258 (70.54%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++ATG+ FA KIIDK R KE LIE E+ +L+ HP+I++L E ETP+++FLV E +K GDLFDAI + K+ E ++ M+ ++ A+ Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PL+ +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q ELF+ I G+ E+ P WD IS +K LI+ ML V+ RFT + L HPW+
Sbjct: 428 YKVGKVIGDGNFAVVKECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLFTVCGTPTYVAPEIIAETGYGLKVDIWAAGVIAYILLCGFPPFRSENNVQEELFDQILRGKLEFPSPDWDTISLPAKMLISQMLQVNVDARFTAEEVLSHPWV 685 HSP 2 Score: 68.5514 bits (166), Expect = 3.251e-11 Identity = 28/78 (35.90%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
KP+++T+IR+G+KP ++LLNK ++ S++ +L+DI++A +++R+Y +DGK++ DFF F+A G
Sbjct: 217 KPKLVTVIRSGVKPRKAVRVLLNKKTAHSFDQVLADITEAI--KLDSGAVKRLYTLDGKQLTCLQDFFGDDDVFMACG 292 HSP 3 Score: 54.6842 bits (130), Expect = 5.311e-7 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
R+ K+V F NG+R F G + +++ +R+ + LL +LT+ ++ +P GVR IY +G ++ +D+L Y+C + + DY
Sbjct: 72 RRAKKVRFYRNGDRYFKGLVYAVSSDRFRSYDALLMELTRSLADNLHLPQGVRSIYTIDGSKKITSMDELVEGECYVCASNQPFRKVDY 160
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Match: ENSORLT00000031333.1 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659]) HSP 1 Score: 293.508 bits (750), Expect = 2.501e-85 Identity = 138/258 (53.49%), Postives = 182/258 (70.54%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLL-CSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G IG GNF+ VKE ++ATG+ FA KIIDK R KE LIE E+ +L+ HP+I++L E ETP+++FLV E +K GDLFDAI + K+ E ++ M+ ++ A+ Y+H IVHRDIKPENLL C +G +LKL DFGLA ++ PL+ +CGTPTYVAPEI++E GYG++VDIWA+GVI YILLCGFPPFRS Q ELF+ I G+ E+ P WD IS +K LI+ ML V+ RFT + L HPW+
Sbjct: 428 YKVGKVIGDGNFAVVKECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLFTVCGTPTYVAPEIIAETGYGLKVDIWAAGVIAYILLCGFPPFRSENNVQEELFDQILRGKLEFPSPDWDTISLPAKMLISQMLQVNVDARFTAEEVLSHPWV 685 HSP 2 Score: 68.5514 bits (166), Expect = 3.251e-11 Identity = 28/78 (35.90%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG 959
KP+++T+IR+G+KP ++LLNK ++ S++ +L+DI++A +++R+Y +DGK++ DFF F+A G
Sbjct: 217 KPKLVTVIRSGVKPRKAVRVLLNKKTAHSFDQVLADITEAI--KLDSGAVKRLYTLDGKQLTCLQDFFGDDDVFMACG 292 HSP 3 Score: 54.6842 bits (130), Expect = 5.311e-7 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSN--FIPTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
R+ K+V F NG+R F G + +++ +R+ + LL +LT+ ++ +P GVR IY +G ++ +D+L Y+C + + DY
Sbjct: 72 RRAKKVRFYRNGDRYFKGLVYAVSSDRFRSYDALLMELTRSLADNLHLPQGVRSIYTIDGSKKITSMDELVEGECYVCASNQPFRKVDY 160
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Match: dclk1a (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101167140]) HSP 1 Score: 280.026 bits (715), Expect = 1.282e-80 Identity = 132/262 (50.38%), Postives = 183/262 (69.85%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLG 3167
Y++G IG GNF+ V+E + +TG+ +A KII+K + + KE +I+ E+ IL+ +HPNIV L E+ +T N+++LV E +K GDLFDAI ++ E ++ M+ ++ A+ Y+HS IVHRDIKPENLL +G +LKL DFGLA + PLY +CGTPTYVAPEI+SE GYG++VDIWA+GVI YILLCGFPPFR +Q LF+ I G+ E+ PYWD +S +K+LI +ML V+ +R+T L L HPW+ G
Sbjct: 382 YKVGRMIGDGNFAVVRECVELSTGREYALKIINKGKCRGKEHMIQNEVAILRRVKHPNIVLLIEEVDTYNELYLVMELVKGGDLFDAITSANRYTERDASGMLHNLANAIKYLHSLNIVHRDIKPENLLVYEHADGSKSLKLGDFGLATVVDGPLYTVCGTPTYVAPEIISETGYGLKVDIWAAGVITYILLCGFPPFRGCSDDQEALFDQILMGQLEFPFPYWDNVSETAKELIRSMLEVEVDQRYTALQVLEHPWVTNEG 643 HSP 2 Score: 69.3218 bits (168), Expect = 1.856e-11 Identity = 34/96 (35.42%), Postives = 56/96 (58.33%), Query Frame = 3
Query: 726 KPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG------DKDPNLNQEEVR 995
+P+++T++R+G+KP +ILLNK ++ SYE +L+DI+DA +++IY ++GK + DFF FVA G D L++ E R
Sbjct: 178 RPKLVTVVRSGVKPRKAVRILLNKKTAHSYEQVLTDITDAI--KLDSGVVKKIYTLEGKLVSCLQDFFGDEDIFVACGPEKFRYQDDLMLDESECR 271 HSP 3 Score: 57.3806 bits (137), Expect = 7.199e-8 Identity = 31/89 (34.83%), Postives = 51/89 (57.30%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNGG-QVQDIDDLEHRNIYICCGQESLKTYDY 455
++ K+V F NG+R F+G + +I+ +R+ + LL DLT+ S+ + P GVR IY +G ++ +D L Y+C E+ K DY
Sbjct: 47 KRAKKVRFYRNGDRYFNGIVYAISADRFRTFDALLADLTRSLSDNVNLPQGVRTIYALDGSKKISSVDQLVEGGSYVCSSTEAYKKLDY 135
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Match: dclk1b (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101155370]) HSP 1 Score: 278.485 bits (711), Expect = 5.118e-80 Identity = 128/258 (49.61%), Postives = 184/258 (71.32%), Query Frame = 3
Query: 2385 YQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWI 3155
Y++G ++G GNF+ V+E +++TG+ +A KII K++ K KE +I+ E+ IL+ +HPNIV L E+ ++P++++LV E +K GDLF AI + K+ E ++ M+ ++ A+ Y+HS IVHRDIKPENLL ++G +LKL DFGLA + PLY +CGTPTYVAPEI++E GYG++VD+WA+G+I YILLCGFPPFRS E +Q LFE I +F++ PYWD +S +K LI ML V +R+T L HPW+
Sbjct: 386 YKVGRTLGDGNFAVVRECVERSTGREYALKIISKEKCKGKEHMIQSEVAILRRVKHPNIVLLVEEIDSPSELYLVMELVKGGDLFYAITSSNKYTERDASCMLFNLANAIKYLHSLNIVHRDIKPENLLVFEHQDGSKSLKLGDFGLATIVNGPLYAVCGTPTYVAPEIVAETGYGLKVDVWAAGIITYILLCGFPPFRSSEDDQEALFEQILKCQFDFPTPYWDNVSDAAKTLITKMLEVSVDQRYTAAQVLDHPWV 643 HSP 2 Score: 98.9821 bits (245), Expect = 1.256e-20 Identity = 75/275 (27.27%), Postives = 119/275 (43.27%), Query Frame = 3
Query: 198 RKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFI--PTGVRRIYDRNG-GQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIG------DKDPNLNQEEVR 995
+K K++ F NG+R F G + +I+ R+ L+ LL DLT+ S+ + P GVR IY +G ++ +D L Y+C E K DY NP + A + S + P F N+ P E+ +P+++TI+R+G+KP +ILLNK ++ S+E +++DI+DA ++R+Y +DGK + DFF F A G D NL++ E R
Sbjct: 49 KKAKKIRFYRNGDRYFKGIVYAISSERFGSLDALLADLTRCLSDNVNLPQGVRTIYTIDGVTKIASMDQLVEGESYVCASIEPFKKLDYTKNV--------------------NPN-WSLGAKTAASVRDPPFLCSNK----------------------------AGSPETRESKDFIRPKLVTIVRSGVKPRKAVRILLNKKTAHSFEQVMTDITDAI--KLDSGVVKRLYTVDGKMVTCLQDFFGEDDIFFACGPEKFRYQDDFNLDENECR 272
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Match: SMESG000072629.1 (SMESG000072629.1) HSP 1 Score: 2023.82 bits (5242), Expect = 0.000e+0 Identity = 1042/1052 (99.05%), Postives = 1046/1052 (99.43%), Query Frame = 3
Query: 126 MSSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFIPTGVRRIYDRNGGQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELIHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSPDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEHTSPGYPNNDSRKPGPFEMAPEESSARILTPKMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNKIPLSNKKFREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNAXXXXXXXXXXXXXXDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEPGHHKYNIAFEVNLLDTDEEKTVKHSNPSLESAKQSLKEPNIGXXXXXXXXXXXXXXXEIPKKLSLAGLIGPRSETVNLFMKGKSFQKPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS 3281
MSSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFIPTGVRRIYDRNG QVQDIDDLEHRNIYICCGQESLKTYDYDTKYFEL+HKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYPISSTPNTSSSNKPSPRSPMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSS DASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEE+TSPGYPNNDSRKPGPFEMAPEESSARILT KMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNK+PLSNK FREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNANQNVNPYNKKVKKPDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEP HHKYNIAFEVNLLDTDEEKTVK SNPSLESAKQSLKEPNIGKSISDLKVKKDSLKSEIPKKLSLAGLIGPRSETVNLFMKGKSF+KPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS
Sbjct: 1 MSSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNRYFILNDLLDDLTKYFSNFIPTGVRRIYDRNGWQVQDIDDLEHRNIYICCGQESLKTYDYDTKYFELLHKGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYPISSTPNTSSSNKPSPRSPMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSRDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEYTSPGYPNNDSRKPGPFEMAPEESSARILTSKMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNKMPLSNKIFREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNANQNVNPYNKKVKKPDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEPSHHKYNIAFEVNLLDTDEEKTVKRSNPSLESAKQSLKEPNIGKSISDLKVKKDSLKSEIPKKLSLAGLIGPRSETVNLFMKGKSFKKPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS 1052
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Match: SMESG000072629.1 (SMESG000072629.1) HSP 1 Score: 1774.99 bits (4596), Expect = 0.000e+0 Identity = 924/933 (99.04%), Postives = 927/933 (99.36%), Query Frame = 3
Query: 483 KGHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYXXXXXXXXXXXXXXXXXXXMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSPDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEHTSPGYPNNDSRKPGPFEMAPEESSARILTPKMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNKIPLSNKKFREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNAXXXXXXXXXXXXXXDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEPGHHKYNIAFEVNLLDTDEEKTVKHSNPSLESAKQSLKEPNIGXXXXXXXXXXXXXXXEIPKKLSLAGLIGPRSETVNLFMKGKSFQKPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS 3281
K HLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYPISSTPNTSSSNKPSPRSPMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSS DASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEE+TSPGYPNNDSRKPGPFEMAPEESSARILT KMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNK+PLSNK FREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNANQNVNPYNKKVKKPDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEP HHKYNIAFEVNLLDTDEEKTVK SNPSLESAKQSLKEPNIGKSISDLKVKKDSLKSEIPKKLSLAGLIGPRSETVNLFMKGKSF+KPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS
Sbjct: 22 KRHLHNHNSHIKFYYNPQEYFDNADSTISPQTPLFYRGNRLYPISSTPNTSSSNKPSPRSPMNRLEERTYKPPIAETNMKSKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNYEKMSDHSADDSRLVKDNKTKFNNMEMTLPRTDEDHLYSSRDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEYTSPGYPNNDSRKPGPFEMAPEESSARILTSKMRAQIFGLERIRAGLEEREKARFLMAKKLDQRNPSKLSPNKYSFNNNPGNKMPLSNKIFREKYKYSPYLESMDGYRNNKENFQLSKIPKSQRSKKPLDFVDNNANQNVNPYNKKVKKPDSDRNQMRNQKNNQVLLNNRNTDTQDKKKQPWERIPPINLGRFTPRNDFNENFHQLPDIRVSLPTLTEENADSSNKNDQKPLDLVEPSHHKYNIAFEVNLLDTDEEKTVKRSNPSLESAKQSLKEPNIGKSISDLKVKKDSLKSEIPKKLSLAGLIGPRSETVNLFMKGKSFKKPTRTKKQSSVLQNPLSQRIVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNSIRFSKPIKDVVTETRVELQMETAIFKSNNTISKNTS 954
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Match: SMESG000060309.1 (SMESG000060309.1) HSP 1 Score: 293.123 bits (749), Expect = 2.729e-85 Identity = 128/269 (47.58%), Postives = 193/269 (71.75%), Query Frame = 3
Query: 2373 FEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLCSIK-NGLINLKLSDFGLAVEIK-EPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKPYWDEISPESKDLINNMLIVDPLKRFTVLDCLCHPWIITLGNS 3173
F Y++G IG G+++ V+E ATG+ A K+ DKKRL + D++++E+ I ++C HPNI +L +ET +VF+V E +K GDLFD+I NI+F E +++++ D+ AL Y+HS+ +VHRDIKPEN+L S +G I++KL+DFGLA ++ +++CGTPTY+APEIL E+GYG EVD WA GVI YIL+ GFPPFR+P+R QTEL++FI G ++Y +W+ IS E++ LI+++L+ P +R+T D +C W++TLG +
Sbjct: 426 FLSKYRMGKKIGEGHYAVVREGSHLATGREVAIKVYDKKRLTNAMDMVDDEVEIQRACNHPNIARLYAAYETEAEVFMVMELVKGGDLFDSIVRNIRFSEETASMIIHDLASALYYLHSRNVVHRDIKPENILISEDIHGRISIKLTDFGLATILRGNQAHLVCGTPTYIAPEILEEKGYGTEVDNWALGVILYILISGFPPFRTPDRQQTELYDFITKGVYDYPLEFWENISMEARFLIDHLLVTHPRERYTARDVICDSWVLTLGGT 694 HSP 2 Score: 53.5286 bits (127), Expect = 1.001e-6 Identity = 24/101 (23.76%), Postives = 61/101 (60.40%), Query Frame = 3
Query: 723 SKPRILTIIRTGIKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNWKGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAEL-FPN 1022
S +++T++R G P + + L+ + + ++ D+L++IS + + + + +++N+ G+EI D F++ F+A+G + L+++ V+ I+ ++ +PN
Sbjct: 91 SGSKMITVVRAGPPPRAIIRALIKRANHKNMADVLTEISQSLVQSSYKERVTKLFNLLGEEILDAKDLFKAEDVFIAVGKE--GLSRKIVKEILEDIYYPN 189
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1) HSP 1 Score: 286.189 bits (731), Expect = 5.207e-81 Identity = 134/240 (55.83%), Postives = 177/240 (73.75%), Query Frame = 3
Query: 2331 IVPVLAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKP 3047
++ L +QL + + + +++ +G GNF+ VK A + T +A KIIDK R+ KE +IE EI I+K C H NIVKL+E++ET +VFLV E +K GDLFDAI +N+KF E +A M+ D+ AL Y+HS+ IVHRD+KPENL+ K+G I LKL+DFGLA+EIK+ +Y +CGTPTYVAPEILSE+GY +EVD+WA GVI YILLCGFPPFRS RNQ ELF+ I++G FE+I P
Sbjct: 728 VMGKLCRQLSSSDIIYQRFEVTKMLGDGNFAVVKLALHRKTKTEYALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMVLRHKDGSITLKLADFGLAMEIKKLIYTVCGTPTYVAPEILSEKGYSLEVDLWALGVITYILLCGFPPFRSSNRNQEELFQIIQSGNFEFISP 967 HSP 2 Score: 88.1965 bits (217), Expect = 2.142e-17 Identity = 74/286 (25.87%), Postives = 133/286 (46.50%), Query Frame = 3
Query: 714 NMKSKPRILTIIRTG-IKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNW--KGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNY------------EKMSDHSADDSRLVKDNKTKFNNMEMTLPRTD---------EDHLYSS----PDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEHTSPGYPNNDSRKPGPFEMAPEESSARILTPKMRAQIFGLERIRAGLEEREKARFLMAKKL 1487
N + KP+++T++R K F +ILLNK S QSYE LLSDI + + K + I RI+N+ GKEI+ DFFR+ +F+A+GD + L +++++I+ +L+P++ +K+ + A D + + + M++ + D +DH ++S D+S + P +S S+R ++ + +PN + N + ++ S A ++ + Q +RIR EEREKAR + ++L
Sbjct: 130 NQQVKPKVITVVRNSPEKSKGFVRILLNKRSVQSYETLLSDIETSLRDHLHHKMQGIGRIFNLFGKEIKSVGDFFRNDDFFIAVGDNE-TLGVKQIKDILDDLYPDHPQTSHVLKYWTSKKVDELPAKDEQRDHEKLKSLHGSTMSVDKRDSGFEEQNFFKDHDFNSNTNVNDSSETVSLPKPKLSKTKPSVRKKNQKIQQNVPNIQ--------------------------NTTESISSLDLDKSNSRASPISNQKSVQERNSDRIRIAYEEREKARIQIQEQL 388
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1) HSP 1 Score: 285.034 bits (728), Expect = 1.644e-80 Identity = 134/236 (56.78%), Postives = 175/236 (74.15%), Query Frame = 3
Query: 2343 LAQQLETDEDFEKYYQLGNSIGSGNFSEVKEAKKKATGKPFAAKIIDKKRLKDKEDLIEEEIRILKSCEHPNIVKLEEQFETPNKVFLVQEYIKEGDLFDAICENIKFPESQSAVMMRDIFRALLYIHSKGIVHRDIKPENLLC-SIKNGLINLKLSDFGLAVEIKEPLYVICGTPTYVAPEILSEQGYGIEVDIWASGVICYILLCGFPPFRSPERNQTELFEFIKAGEFEYIKP 3047
L +QL + + + +++ +G GNF+ VK A + T +A KIIDK R+ KE +IE EI I+K C H NIVKL+E++ET +VFLV E +K GDLFDAI +N+KF E +A M+ D+ AL Y+HS+ IVHRD+KPENL+ K+G I LKL+DFGLA+EIK+ +Y +CGTPTYVAPEILSE+GY +EVD+WA GVI YILLCGFPPFRS RNQ ELF+ I++G FE+I P
Sbjct: 742 LCRQLSSSDIIYQRFEVTKMLGDGNFAVVKLALHRKTKTEYALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMVLRHKDGSITLKLADFGLAMEIKKLIYTVCGTPTYVAPEILSEKGYSLEVDLWALGVITYILLCGFPPFRSSNRNQEELFQIIQSGNFEFISP 977 HSP 2 Score: 88.1965 bits (217), Expect = 2.307e-17 Identity = 74/286 (25.87%), Postives = 133/286 (46.50%), Query Frame = 3
Query: 714 NMKSKPRILTIIRTG-IKPYSFFKILLNKTSSQSYEDLLSDISDAFGSNW--KGKSIQRIYNIDGKEIQQTSDFFRSGKYFVAIGDKDPNLNQEEVRNIVAELFPNY------------EKMSDHSADDSRLVKDNKTKFNNMEMTLPRTD---------EDHLYSS----PDASYRKTDVHPNMSNHNVSIRPNHKSTRDQIPNKKRNGKKPYQQLLAPYYSEEHTSPGYPNNDSRKPGPFEMAPEESSARILTPKMRAQIFGLERIRAGLEEREKARFLMAKKL 1487
N + KP+++T++R K F +ILLNK S QSYE LLSDI + + K + I RI+N+ GKEI+ DFFR+ +F+A+GD + L +++++I+ +L+P++ +K+ + A D + + + M++ + D +DH ++S D+S + P +S S+R ++ + +PN + N + ++ S A ++ + Q +RIR EEREKAR + ++L
Sbjct: 156 NQQVKPKVITVVRNSPEKSKGFVRILLNKRSVQSYETLLSDIETSLRDHLHHKMQGIGRIFNLFGKEIKSVGDFFRNDDFFIAVGDNE-TLGVKQIKDILDDLYPDHPQTSHVLKYWTSKKVDELPAKDEQRDHEKLKSLHGSTMSVDKRDSGFEEQNFFKDHDFNSNTNVNDSSETVSLPKPKLSKTKPSVRKKNQKIQQNVPNIQ--------------------------NTTESISSLDLDKSNSRASPISNQKSVQERNSDRIRIAYEEREKARIQIQEQL 414 The following BLAST results are available for this feature:
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase DCLK3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032800 ID=SMED30032800|Name=Serine/threonine-protein kinase DCLK3|organism=Schmidtea mediterranea sexual|type=transcript|length=3366bpback to top protein sequence of SMED30032800-orf-1 >SMED30032800-orf-1 ID=SMED30032800-orf-1|Name=SMED30032800-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1053bp MSSYIKYNPSQSFRDITDDYYQELRKPKRVLFIVNGNRTFSGKLLSITPNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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