SMED30032789

Overview
NameSMED30032789
Smed IDSMED30032789
Length (bp)1816
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032789 (SMED30032789) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032789 (SMED30032789) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032789 (SMED30032789) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032789 (SMED30032789) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032789

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
GABAergic neuronSMED30032789SMESG000038917.1 SMESG000001725.1 SMESG000063380.1 dd_Smed_v6_6535_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
ovarySMED30032789SMESG000053363.1 SMESG000053360.1 SMESG000001725.1 Contig2831newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30032789SMESG000053363.1 SMESG000053360.1 SMESG000001725.1 Contig2831uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30032789SMESG000053363.1 SMESG000053360.1 SMESG000001725.1 Contig2831newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30032789SMESG000053363.1 SMESG000053360.1 SMESG000001725.1 Contig2831uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30032789SMESG000011789.1 Contig2831newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30032789SMESG000011789.1 Contig2831uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30032789SMESG000011789.1 Contig2831newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30032789SMESG000011789.1 Contig2831uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032789 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002980:27136..28806 +7066
Homology
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BLAST of SMED30032789 vs. UniProt/SwissProt
Match: sp|P34457|YMD3_CAEEL (Putative uncharacterized transposon-derived protein F54H12.3 OS=Caenorhabditis elegans OX=6239 GN=F54H12.3 PE=5 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.359e-11
Identity = 48/188 (25.53%), Postives = 91/188 (48.40%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEAS----NNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            +R  +   L +K+G+ + + ++ +F+  +      +Y D G+EFYN   ++ L +N + ++S    P    +      I+ER NR LK+ + K  T++ NR + DVL  ++   NN ++R I   P +      + P  +  +             K KF +G+ VRI   + +F+KG    W+ E++
Sbjct:   22 SRRLFVKTLKSKKGQEVAEALQDVFK-EIGVTPMTIYSDDGKEFYNSHVKELLAKNFVNLFS----PKSEIKCA----IVERANRTLKTRLAKYMTQKYNRRYVDVLQQVVNGINNSVNRGIGKKPVDVERGDFSTPMPVDSV-------------KIKFRVGDHVRIAAKRGQFDKGYEQGWTTEVY 187
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BLAST of SMED30032789 vs. TrEMBL
Match: B7S8P0 (Integrase core domain-containing protein OS=Glyptapanteles flavicoxis OX=463051 GN=GFP_L5_0130 PE=4 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 6.446e-29
Identity = 72/186 (38.71%), Postives = 108/186 (58.06%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTE--ASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            ++YAWA+P+ +K G  +   ++ + +    RI   L+ D G+EFYNK F+D ++++ I +YSTF+             I ERFNR LK+ M++ FT RG+  W D+LD L+  YNN  HR IK  P +  A+    L+ +I      A     +K KF +G++VR+ K+KN FEKG T  W+ EIF
Sbjct:   62 SKYAWAIPIKSKSGADVTSAMKSVLQQG--RIPKNLHVDQGKEFYNKEFKDLMKQHNINIYSTFS--------NLKASICERFNRTLKTKMWREFTARGSYKWTDILDDLVNTYNNTKHRTIKMNPADVTAAQEKQLLEKIYQQIQPAP----KKNKFKVGDKVRVSKYKNVFEKGYTPNWTTEIF 233
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BLAST of SMED30032789 vs. TrEMBL
Match: A0A5E4N069 (Ribonuclease H-like domain,Chromo/chromo shadow domain,Integrase, catalytic core,Chromo domain-like OS=Cinara cedri OX=506608 GN=CINCED_3A005480 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 7.650e-27
Identity = 74/184 (40.22%), Postives = 97/184 (52.72%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            T+YAWA+PL +K  + +   +  I    +NR    L  D G+EFYN TF   +++  +  YSTF+        T+   I+ERFNR LK  M++ FT RGN  W  +L SLI  YNN  HR I   P EA NNP  ++ I  GN     N     KF +G+ VR+   K  F KG    WS EIF
Sbjct:   22 TKYAWAIPLKSKTAKEVTHAMSTIL---INRSPQLLQLDNGKEFYNSTFDTLMKKYNVKKYSTFS--------TTKACIVERFNRTLKEKMFREFTARGNHNWLSILSSLINEYNNSKHRTIGMIPLEADNNPGKVK-IKRGNIPTKIN-----KFKVGDSVRVSTQKGVFTKGYLPNWSTEIF 188
[Close]
BLAST of SMED30032789 vs. TrEMBL
Match: A0A2B4RCZ4 (Uncharacterized transposon-derived protein F54H12.3 OS=Stylophora pistillata OX=50429 GN=F54H12.3 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 3.089e-26
Identity = 71/187 (37.97%), Postives = 111/187 (59.36%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEAS--NNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNK-FEKGSTHKWSKEIF 552
            ++Y W VPL NK+GE++ +  EKIF     R+  +L+ D G E+YN   +D ++  G+ MYST N+          + + ER+NR +K+ M+K FT + N  + D+L  ++  YNN+ HR I  TP EAS   N + +     G+ + ++ L E+ KF +G+ VRI K+K K F+KG T  W++EIF
Sbjct:  215 SKYGWIVPLKNKKGESVAESFEKIFTEG--RVPKRLWVDKGSEYYNSHVKDLMKSEGVEMYSTENE--------EKSSVCERWNRTIKTEMWKQFTIQNNTQYLDILPKIVKKYNNRKHRSIGMTPVEASKKKNENAVFLKLYGDLSESERLREQSKFAVGDHVRISKFKRKVFDKGFTPNWTEEIF 391
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BLAST of SMED30032789 vs. TrEMBL
Match: A0A158P4E7 (Uncharacterized protein OS=Tetranychus urticae OX=32264 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 4.830e-26
Identity = 70/184 (38.04%), Postives = 107/184 (58.15%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            TRYAWA+P+ +K   T+    EKIFES+  R   K+  D G+EF N  F+ FL ++G I +++       T       I+ERFNR L+S +Y+      ++ + D LD +++ YN+  HR IK +P EA +NP+  R   N       NL +++ + +G+ VRI + K+ FEKGST  W++EIF
Sbjct:  105 TRYAWAIPIKDKSSSTVISAFEKIFESS-KRKPLKVQTDQGKEFVNHNFKSFLSQHGSIHFTS-------TDDVIKCAIVERFNRTLRSRIYRYLHYTNSQRYMDALDDIVHGYNSSYHRTIKMSPIEALDNPEGAR--VNIRKAQPINLKKQKPYKVGDTVRITRAKSIFEKGSTKNWTEEIF 278
[Close]
BLAST of SMED30032789 vs. TrEMBL
Match: X1WLU8 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 1.435e-25
Identity = 74/184 (40.22%), Postives = 99/184 (53.80%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            T+Y+WAV L  K+GE +     KIF  N     N L+ D G EFYNK F+  +++  I MYSTF+             I+ERFNR LK  M+K FT RG+R W  +L +LI  YNN +HR I  TP +A   P     +T  ++        K KF++G++VRI  +K  F K     WS EIF
Sbjct:  255 TKYSWAVGLKTKKGEEVAAAASKIFAQNS---PNLLHVDRGREFYNKHFEALVKKLNIKMYSTFS--------AVKASIVERFNRTLKQRMFKEFTSRGSRDWITILPALIDDYNNSMHRTIGMTPAQADARPS---RVTLKHDVVK---TRKIKFHVGDKVRISVYKGVFTKAYLPNWSTEIF 421
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BLAST of SMED30032789 vs. Ensembl Cavefish
Match: ENSAMXT00000055500.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001032.1:12601:13692:-1 gene:ENSAMXG00000041341.1 transcript:ENSAMXT00000055500.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 3.322e-18
Identity = 61/187 (32.62%), Postives = 95/187 (50.80%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNP-IIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEA--SNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            ++YAW   L NK G  + D    I +    R+  KL  D G+EF+NK FQ  ++++ I+ ++T         GT     + ERFNR L++ M+K FT    R + DV+  L++ YN+  H  IK  P E   +N+  + + +     +    L  + KF IG+ VRI + K  FEKG    ++ E F
Sbjct:  104 SKYAWVRVLRNKTGVEVTDAFNSILKEG--RVPKKLQTDQGKEFFNKNFQGLMKKHNIVHFAT---------GTGQKASVCERFNRTLRNKMWKYFTAVNTRKYIDVVQELVHSYNHNYHSSIKMKPVEVDETNSFKVFKTLYGLFPSRVKKL--RFKFKIGDVVRISRLKAHFEKGYEQNYTDEYF 277
[Close]
BLAST of SMED30032789 vs. Planmine SMEST
Match: SMESG000067088.1 (SMESG000067088.1)

HSP 1 Score: 235.343 bits (599), Expect = 1.565e-99
Identity = 121/184 (65.76%), Postives = 138/184 (75.00%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            TRYA  +PL  K   +IK+  E +FE   NRI  KLY D  +EFYNK F++F QEN I +YSTFN PD+R+   SHNPIIER NR LK+ MYK FTE GNRIW DVL  LI  YNNKIHR I  +PTEAS NPDLI+EI + NN AN+NL EKQKFNI +RVRIFKWKNKFEKGSTHKW +EIF
Sbjct:  279 TRYATVLPLKTKTANSIKEGFEFLFEETPNRIPKKLYVDNSKEFYNKIFEEFRQENNIEIYSTFNSPDDRSNYGSHNPIIERLNRKLKNWMYKKFTECGNRIWIDVLPELINKYNNKIHRTIGVSPTEASENPDLIKEIIHDNNYANENLQEKQKFNIDDRVRIFKWKNKFEKGSTHKWFREIF 462

HSP 2 Score: 148.288 bits (373), Expect = 1.565e-99
Identity = 84/131 (64.12%), Postives = 91/131 (69.47%), Query Frame = -3 
Query:  582 AAALKHDIVYCQEDICSRHKADIDLIQDLKDIRNLTFSERFSRAIGKIAMKTKIMLGG-------EIPTNSDNLNTTLANELHQREAFATELHKEYRKPKKYLKIKVL-------IDLIETVNPKNNKSYR 932
            A ALKHDI Y Q+DI  R KADIDLIQDL DI+N TFSERFSRAIGKIAM+TKI L G       E+PTNSDN NT L N          ELHK YRKPKKYL+IKV         DLIET+NPKNNK+YR
Sbjct:  149 AVALKHDIAYRQKDIRFRQKADIDLIQDLNDIQNPTFSERFSRAIGKIAMETKIALRGAQATVFEEMPTNSDNQNTNLVN----------ELHKGYRKPKKYLRIKVFNKDDIWSADLIETINPKNNKNYR 269
[Close]
BLAST of SMED30032789 vs. Planmine SMEST
Match: SMESG000068171.1 (SMESG000068171.1)

HSP 1 Score: 245.743 bits (626), Expect = 3.869e-92
Identity = 123/183 (67.21%), Postives = 143/183 (78.14%), Query Frame = -2 
Query:    1 RYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 549
            RYAW +PL  K   +IK+  E +FE   +RI  KLY D G+EFYNK F++F Q+N I +YSTFN PD+R+   SHNPIIERFNR LK+ MYK FTE+GNRIW DVL  LI  YNNKIHR I  + TEAS NPDLI+EIT+ NN AN++L EKQKFNIG+RVRIFKWKNKFEKGSTHKWSKEIF
Sbjct:  391 RYAWVLPLKTKTAHSIKEGFEFLFEETPDRIPKKLYVDNGKEFYNKIFEEFRQQNNIEIYSTFNSPDDRSNYGSHNPIIERFNRTLKNWMYKKFTEQGNRIWIDVLPELIIKYNNKIHRTIGVSTTEASENPDLIKEITHENNYANEDLQEKQKFNIGDRVRIFKWKNKFEKGSTHKWSKEIF 573

HSP 2 Score: 113.235 bits (282), Expect = 3.869e-92
Identity = 77/106 (72.64%), Postives = 81/106 (76.42%), Query Frame = -3 
Query:  582 IQDLKDIRNLTFSERFSRAIGKIAMKTKIMLGG-------EIPTNSDNLNTTLANELHQREAFATELHKEYRKPKKYLKIKVL-------IDLIETVNPKNNKSYR 857
            +Q L DI+N TFSERFSRAIGKIAMKTKI L G       EIPTNSDN NTTLANELH RE  ATELHKEY+KPKKYLKIKV         DLIETVNPKNNK+YR
Sbjct:  275 VQYLNDIQNPTFSERFSRAIGKIAMKTKIALVGAQATAFEEIPTNSDNQNTTLANELHHREVLATELHKEYKKPKKYLKIKVFNKDDIWSADLIETVNPKNNKNYR 380
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BLAST of SMED30032789 vs. Planmine SMEST
Match: SMESG000015236.1 (SMESG000015236.1)

HSP 1 Score: 181.8 bits (460), Expect = 3.357e-91
Identity = 97/131 (74.05%), Postives = 101/131 (77.10%), Query Frame = -3 
Query:  582 AAALKHDIVYCQEDICSRHKADIDLIQDLKDIRNLTFSERFSRAIGKIAMKTKIMLGG-------EIPTNSDNLNTTLANELHQREAFATELHKEYRKPKKYLKIKVL-------IDLIETVNPKNNKSYR 932
            AAALK DI Y QEDI SR KADIDLIQDL DI+N TFSERFSRAIGKIAMKTKI LGG       EIPTNSDN NTTL NELH RE  ATELHKEYRKPKKYLKIKV         DLIE +NPKNNK+Y+
Sbjct:  120 AAALKPDIGYRQEDIRSRQKADIDLIQDLNDIQNPTFSERFSRAIGKIAMKTKIALGGAQATVFEEIPTNSDNQNTTLVNELHHREVLATELHKEYRKPKKYLKIKVFNKDDIWSADLIEIINPKNNKNYK 250

HSP 2 Score: 173.711 bits (439), Expect = 3.357e-91
Identity = 100/184 (54.35%), Postives = 119/184 (64.67%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            TRY W +PL  KQ   +K       +SN++           EEFYNK F++F QEN I                      ERFNR LK+ MYK FTE+GNRIW +VL  LI  YNNKIHR I  +P EAS+NPDLI+EIT+ NN AN++L EKQKFNIG+RVRIFKWKNKF+KGST+KWSKEIF
Sbjct:  260 TRYVWVLPLKKKQEIQLKK------DSNVD----------NEEFYNKIFEEFRQENDI----------------------ERFNRTLKNWMYKKFTEQGNRIWVNVLPELINKYNNKIHRTIGVSPREASDNPDLIKEITHDNNYANEDLQEKQKFNIGDRVRIFKWKNKFDKGSTYKWSKEIF 405
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BLAST of SMED30032789 vs. Planmine SMEST
Match: SMESG000037169.1 (SMESG000037169.1)

HSP 1 Score: 194.512 bits (493), Expect = 2.103e-88
Identity = 105/184 (57.07%), Postives = 123/184 (66.85%), Query Frame = -2 
Query:    1 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTEASNNPDLIREITNGNNNANDNLIEKQKFNIGNRVRIFKWKNKFEKGSTHKWSKEIF 552
            TRYAWA+PL  K   +IK+  E +FE   NRI  KLY D G+E YNK F+DF QEN I +YSTFN PD+R+                         E+GNRIW +VL  LI  +NNKIHR I  +P EAS NPDLIREIT+ NN AN++L EKQ+FNIG+RVRIFKWKNKFEKGSTH WSKE F
Sbjct:  127 TRYAWALPLKTKTTNSIKEGFEFLFEETSNRIPKKLYVDNGKEIYNKIFEDFKQENDIEIYSTFNSPDDRSN-----------------------YEQGNRIWINVLPELINKHNNKIHRTIGVSPREASENPDLIREITHENNYANEDLQEKQRFNIGDRVRIFKWKNKFEKGSTHNWSKENF 287

HSP 2 Score: 151.754 bits (382), Expect = 2.103e-88
Identity = 80/117 (68.38%), Postives = 85/117 (72.65%), Query Frame = -3 
Query:  582 ICSRHKADIDLIQDLKDIRNLTFSERFSRAIGKIAMKTKIMLGG-------EIPTNSDNLNTTLANELHQREAFATELHKEYRKPKKYLKIKVL-------IDLIETVNPKNNKSYR 890
            +CSRH  +IDLIQDL D +N TFSERFSR IGKIA+KTKI L G       EIP NSDN NTTLANELH RE  ATELHKEYRKPKKYLKIKV         D IE VNPKN K+YR
Sbjct:    1 MCSRHNENIDLIQDLNDFQNPTFSERFSRPIGKIAIKTKITLRGAQATVFEEIPANSDNQNTTLANELHNREVLATELHKEYRKPKKYLKIKVFNKDVFWSADFIEIVNPKNKKNYR 117
[Close]
BLAST of SMED30032789 vs. Planmine SMEST
Match: SMESG000064207.1 (SMESG000064207.1)

HSP 1 Score: 196.438 bits (498), Expect = 1.262e-76
Identity = 94/128 (73.44%), Postives = 107/128 (83.59%), Query Frame = -2 
Query:  169 TRYAWAVPLINKQGETIKDPVEKIFESNLNRIHNKLYFDYGEEFYNKTFQDFLQENGIIMYSTFNQPDERTQGTSHNPIIERFNRPLKSLMYK*FTERGNRIWFDVLDSLIYFYNNKIHRLIKTTPTE 552
            TRYAWA+PL+NK+ ETIK+  E +FESN NRI NKLY D G+EFYNK F  FL+EN I MYSTFNQPDERT+G+SHNPIIERFNR LKSLMYK FTERGNRIW D++D LI FYNNKI+R IK +P E
Sbjct:  179 TRYAWAIPLLNKKAETIKEAFETMFESNPNRIPNKLYVDNGKEFYNKLFSQFLKENDIKMYSTFNQPDERTRGSSHNPIIERFNRTLKSLMYKKFTERGNRIWIDLIDDLISFYNNKIYRTIKLSPME 306

HSP 2 Score: 110.538 bits (275), Expect = 1.262e-76
Identity = 68/129 (52.71%), Postives = 78/129 (60.47%), Query Frame = -3 
Query:  582 AALKHDIVYCQEDICSRHKADIDLIQDLKDIRNLTFSERFSRAIGKIAMKTKIMLGG------EIPTNSDNLNTTLANELHQREAFATELHKEYRKPKKYLKIKVLI-------DLIETVNPKNNKSYR 929
            AAL H+I Y  E++  R KADIDLIQDL +I+N TF E FSR I K AMK KI+LGG      E  TN +  N  LAN          ELHKEYR  KKYLKIKV         DLIETVN KNN +++
Sbjct:   51 AALHHNITYRNENLRDRQKADIDLIQDLNEIQNPTFGEIFSREIAKGAMKAKIVLGGGDGRALETSTNEEQENIVLAN----------ELHKEYRNNKKYLKIKVFYKYDIWSADLIETVNSKNNNNFK 169
The following BLAST results are available for this feature:
BLAST of SMED30032789 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P34457|YMD3_CAEEL1.359e-1125.53Putative uncharacterized transposon-derived protei... [more]
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BLAST of SMED30032789 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
B7S8P06.446e-2938.71Integrase core domain-containing protein OS=Glypta... [more]
A0A5E4N0697.650e-2740.22Ribonuclease H-like domain,Chromo/chromo shadow do... [more]
A0A2B4RCZ43.089e-2637.97Uncharacterized transposon-derived protein F54H12.... [more]
A0A158P4E74.830e-2638.04Uncharacterized protein OS=Tetranychus urticae OX=... [more]
X1WLU81.435e-2540.22Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
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BLAST of SMED30032789 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000055500.13.322e-1832.62pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30032789 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032789 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067088.11.565e-9965.76SMESG000067088.1[more]
SMESG000068171.13.869e-9267.21SMESG000068171.1[more]
SMESG000015236.13.357e-9174.05SMESG000015236.1[more]
SMESG000037169.12.103e-8857.07SMESG000037169.1[more]
SMESG000064207.11.262e-7673.44SMESG000064207.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032789 ID=SMED30032789|Name=SMED30032789|organism=Schmidtea mediterranea sexual|type=transcript|length=1816bp
AAAAATTTCTTTAGACCATTTATGTGTTGAACCTTTTTCAAATTTATTTT
TCCATTTGAATATTCTTACTCGATTTCCAATATTGAATTTTTGTTTTTCA
ATTAAATTATCATTTGCATTATTATTTCCATTTGTAATTTCTCTAATTAA
ATCTGGATTATTACTTGCTTCTGTTGGAGTTGTTTTTATCAATCGGTGTA
TTTTATTATTATAAAAATAAATTAGACTATCTAGAACATCAAACCAAATA
CGATTTCCTCTTTCTGTAAATTATTTATACATTAGAGATTTAAGTGGTCT
ATTAAATCGCTCAATAATAGGATTATGTGATGTCCCTTGTGTTCTTTCAT
CTGGCTGATTGAATGTTGAATACATGATAATACCGTTTTCTTGTAAAAAG
TCTTGGAATGTTTTGTTGTAAAATTCTTCACCATAATCAAAATATAATTT
ATTATGGATTCTATTCAGATTTGATTCAAATATTTTTTCAACAGGGTCTT
TAATTGTTTCACCTTGTTTATTTATCAAAGGAACAGCCCAAGCATATCGA
GTATATAAATCAATAATTATTAGAATATATATCTATAACTTTTATTATTT
TTAGGATTTACGGTTTCAATTAAATCTATTAAAACTTTTATTTTTAAATA
TTTCTTAGGTTTTCTATATTCTTTGTGTAACTCTGTTGCAAAAGCTTCTC
GCTGGTGTAACTCATTCGCTAGAGTAGTATTTAGGTTGTCTGAGTTTGTT
GGTATTTCTCCACCTAACATTATCTTTGTTTTCATTGCTATTTTTCCTAT
TGCTCTTGAAAATCTTTCAGAAAATGTTAGATTTCGAATATCCTTTAAAT
CTTGAATCAAATCAATATCTGCTTTATGACGAGAACAAATATCTTCTTGA
CAATACACTATGTCATGTTTTAAAGCCGCTGCTATATTGAGTGTTGAGGA
TATAGATGAAGGAGGGATTACACGTCACTGTCGAATTTTTCGGTATATGC
TGATGATTGTAAAGTGCCAATCTGTGAAATTTTGCAGCAAAATGCGATCA
TCGTGGTTAAAGGTTTTTCCGGATCGTTTTCTTACAGTACCATTTCCAGT
CAGAATTACATCCAGTGGTCCAGACATACCATATCCTAGGAATCTCAAGA
CAGGCTACAAATTTATGCCACTTTGGCAGAAACTAGACATTAAATTAAAG
CCAACGTCCAGCAAAATATCCCAATGCGAGATACGATATGATATGTACTG
TCCAAGTTTAAAACCCAAAATCATTGAATCAAAGGCAAGTCCTGCTAAAT
ATACATGCCATCACATGTATCCATCATGCGACATCGTAATAGTGGTTGGT
CAGGCACTTGTTTTGGGAAACTATTGGATGTTGAAAATGAATTTACAGAA
ACTGAATGTCTGAATACCGATGTTAGCCACAGCAGTTCGTCTGTGTCTCC
AGCTGCCTACAGAAATATCTTTGATATTTTCAAATCGACATGGGAGGACG
AGAATCAAACTTTTAGAAATGACTGTAAAGAATAAAATACAGAATGTCGT
TATCTTTATTAATTATATAGTTTTGAATGGTCTCTTTTATTAACAACATC
CCCTTTCAATTGCAAATCTTTCCAAACTTTATTGAAAGGCCACTATTTTC
CTGATCAGTTTAATACTTCATATTTACTCCTCATAACAAGAATTTTTGAA
TAATTCGTTAAATGCTAATCCTAATGGATTATTGATTTTAAAAAAATTTT
TCTGTTACCTTTTTTATATACTATACATGGTAAGTAAGCGGTATGTCCCC
TATTTTTCCCATTTGA
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protein sequence of SMED30032789-orf-1

>SMED30032789-orf-1 ID=SMED30032789-orf-1|Name=SMED30032789-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp
MSCFKAAAILSVEDIDEGGITRHCRIFRYMLMIVKCQSVKFCSKMRSSWL
KVFPDRFLTVPFPVRITSSGPDIPYPRNLKTGYKFMPLWQKLDIKLKPTS
SKISQCEIRYDMYCPSLKPKIIESKASPAKYTCHHMYPSCDIVIVVGQAL
VLGNYWMLKMNLQKLNV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000464GABAergic neuron
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR001584Integrase_cat-core
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
Vocabulary: biological process
TermDefinition
GO:0015074DNA integration