SMED30032788

Overview


Name	SMED30032788
Smed ID	SMED30032788
Length (bp)	825

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30032788 (SMED30032788) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032788 (SMED30032788) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032788 (SMED30032788) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032788 (SMED30032788) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032788

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
reproductive organ	SMED30032788	SMESG000032301.1	Contig31696	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30032788	SMESG000032301.1	Contig31696	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
neoblast	SMED30032788	SMESG000061809.1 SMESG000061808.1 SMESG000032312.1	SMED_08691_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012
neoblast	SMED30032788	SMESG000061809.1 SMESG000061808.1	SMED_08691_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012

Note: Hover over icons to view figure legend

Alignments

SMED30032788 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000422:131065..131928 +	2748
v31.006314:27010..27914 +	2534

Homology

[Close]

BLAST of SMED30032788 vs. Ensembl Celegans
Match: pig-1 (Maternal embryonic leucine zipper kinase [Source:UniProtKB/Swiss-Prot;Acc:U4PR86])

HSP 1 Score: 47.3654 bits (111), Expect = 1.747e-8
Identity = 42/156 (26.92%), Postives = 80/156 (51.28%), Query Frame = -3

Query:  377 ELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPY--ESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDL------EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  L  LS ++ + +L  YI+  +   +VMEY  G   ++ DY ++     ES  +   + L+  +  +   GY HRD+KP+N+++ E+ L +KLID       E    D++++  C    G+  + +PE+   + Y   ++ V ++G + Y  L
Sbjct:   57 EMDALRNLS-HQNICRLYHYIETEDKFFIVMEYCSGG--EMFDYIVRKERLEESEARHFFRQLVSAIAFVHSQGYAHRDLKPENLLLTED-LHLKLIDFGLCAKTEKGRIDKHNLDTC---CGSPAYAAPELIQGLQYKGNEADVWSMGILLYTLL 205

HSP 2 Score: 29.261 bits (64), Expect = 1.747e-8
Identity = 11/33 (33.33%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S L++  +   +++ P+ RI    LL HDW +H
Sbjct:  235 SPLSKQLLRAMLQVVPERRISVKKLLEHDWLNH 267

[Close]

BLAST of SMED30032788 vs. Ensembl Celegans
Match: pig-1 (Maternal embryonic leucine zipper kinase [Source:UniProtKB/Swiss-Prot;Acc:U4PR86])

HSP 1 Score: 47.3654 bits (111), Expect = 1.761e-8
Identity = 42/156 (26.92%), Postives = 80/156 (51.28%), Query Frame = -3

Query:  377 ELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPY--ESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDL------EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  L  LS ++ + +L  YI+  +   +VMEY  G   ++ DY ++     ES  +   + L+  +  +   GY HRD+KP+N+++ E+ L +KLID       E    D++++  C    G+  + +PE+   + Y   ++ V ++G + Y  L
Sbjct:   57 EMDALRNLS-HQNICRLYHYIETEDKFFIVMEYCSGG--EMFDYIVRKERLEESEARHFFRQLVSAIAFVHSQGYAHRDLKPENLLLTED-LHLKLIDFGLCAKTEKGRIDKHNLDTC---CGSPAYAAPELIQGLQYKGNEADVWSMGILLYTLL 205

HSP 2 Score: 29.261 bits (64), Expect = 1.761e-8
Identity = 11/33 (33.33%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S L++  +   +++ P+ RI    LL HDW +H
Sbjct:  235 SPLSKQLLRAMLQVVPERRISVKKLLEHDWLNH 267

[Close]

BLAST of SMED30032788 vs. Ensembl Celegans
Match: pig-1 (Maternal embryonic leucine zipper kinase [Source:UniProtKB/Swiss-Prot;Acc:U4PR86])

HSP 1 Score: 47.3654 bits (111), Expect = 1.775e-8
Identity = 42/156 (26.92%), Postives = 80/156 (51.28%), Query Frame = -3

Query:  377 ELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPY--ESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDL------EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  L  LS ++ + +L  YI+  +   +VMEY  G   ++ DY ++     ES  +   + L+  +  +   GY HRD+KP+N+++ E+ L +KLID       E    D++++  C    G+  + +PE+   + Y   ++ V ++G + Y  L
Sbjct:   57 EMDALRNLS-HQNICRLYHYIETEDKFFIVMEYCSGG--EMFDYIVRKERLEESEARHFFRQLVSAIAFVHSQGYAHRDLKPENLLLTED-LHLKLIDFGLCAKTEKGRIDKHNLDTC---CGSPAYAAPELIQGLQYKGNEADVWSMGILLYTLL 205

HSP 2 Score: 29.261 bits (64), Expect = 1.775e-8
Identity = 11/33 (33.33%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S L++  +   +++ P+ RI    LL HDW +H
Sbjct:  235 SPLSKQLLRAMLQVVPERRISVKKLLEHDWLNH 267

[Close]

BLAST of SMED30032788 vs. Ensembl Celegans
Match: pig-1 (Maternal embryonic leucine zipper kinase [Source:UniProtKB/Swiss-Prot;Acc:U4PR86])

HSP 1 Score: 47.3654 bits (111), Expect = 1.863e-8
Identity = 42/156 (26.92%), Postives = 80/156 (51.28%), Query Frame = -3

Query:  377 ELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPY--ESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDL------EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  L  LS ++ + +L  YI+  +   +VMEY  G   ++ DY ++     ES  +   + L+  +  +   GY HRD+KP+N+++ E+ L +KLID       E    D++++  C    G+  + +PE+   + Y   ++ V ++G + Y  L
Sbjct:   57 EMDALRNLS-HQNICRLYHYIETEDKFFIVMEYCSGG--EMFDYIVRKERLEESEARHFFRQLVSAIAFVHSQGYAHRDLKPENLLLTED-LHLKLIDFGLCAKTEKGRIDKHNLDTC---CGSPAYAAPELIQGLQYKGNEADVWSMGILLYTLL 205

HSP 2 Score: 29.261 bits (64), Expect = 1.863e-8
Identity = 11/33 (33.33%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S L++  +   +++ P+ RI    LL HDW +H
Sbjct:  235 SPLSKQLLRAMLQVVPERRISVKKLLEHDWLNH 267

[Close]

BLAST of SMED30032788 vs. Ensembl Celegans
Match: nekl-2 (Serine/threonine-protein kinase nekl-2 [Source:UniProtKB/Swiss-Prot;Acc:O01775])

HSP 1 Score: 49.6766 bits (117), Expect = 1.008e-6
Identity = 44/148 (29.73%), Postives = 71/148 (47.97%), Query Frame = -3

Query:  374 PKLITYIDE---NNWSTVVMEYLDG--------DWMDLHDYTMQ--FPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK 778
            P +I YID    +N   +VM+Y +G        D   + D  M+  FP E  +      ++I +  M +    HRD+KP+NI++N     +KL D   +  +       ++ +GT  + SPEI    PYN K  +  ++GCV Y  L+
Sbjct:   63 PLIIGYIDSFIMDNQLGIVMQYAEGGTLERLINDQRAIKDSNMREYFP-EKTVLDYFTQILIALNHMHQKNIVHRDLKPQNILMNRRKTVLKLSDF-GISKELGTKSAASTVIGTPNYLSPEICESRPYNQKSDM-WSLGCVLYELLQ 207

[Close]

BLAST of SMED30032788 vs. Ensembl Fly
Match: fu (gene:FBgn0001079 transcript:FBtr0074602)

HSP 1 Score: 43.5134 bits (101), Expect = 9.490e-6
Identity = 25/87 (28.74%), Postives = 44/87 (50.57%), Query Frame = -3

Query:  362 HRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY--------FCLKSIFH 598
            HRD+KP+N+++ ++ +  KL D            +  S  GT  + +PE+  + PY+   + + ++GC+AY        FC  SI H
Sbjct:  123 HRDLKPQNVLL-DKNMHAKLCDFGLARNMTLGTHVLTSIKGTPLYMAPELLAEQPYD-HHADMWSLGCIAYESMAGQPPFCASSILH 207

HSP 2 Score: 24.2534 bits (51), Expect = 9.490e-6
Identity = 13/33 (39.39%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  273 NLSSELARSFITDCIKLDPDERIEFGDLLSHDW 371
             L+SE  RSF+   ++ DP  RI +  LL H +
Sbjct:  222 TLTSE-CRSFLQGLLEKDPGLRISWTQLLCHPF 253

[Close]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Match: pimr68 (Pim proto-oncogene, serine/threonine kinase, related 68 [Source:ZFIN;Acc:ZDB-GENE-070912-431])

HSP 1 Score: 44.2838 bits (103), Expect = 2.268e-8
Identity = 31/87 (35.63%), Postives = 43/87 (49.43%), Query Frame = -3

Query:  365 GYYHRDIKPKNIMVNEETLEVKLID------LEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLKSIF 607
            G +HRDIK  N++VN ETLEVKLID      L    +D Y         GT  +  PE      Y+ K + V ++G + +  + S F
Sbjct:  117 GVFHRDIKMSNLLVNVETLEVKLIDFGCGDLLRTSSYDTYK--------GTERYCPPEFIEMGEYHGKAATVWSLGVLLFRMITSFF 195

HSP 2 Score: 32.7278 bits (73), Expect = 2.268e-8
Identity = 12/25 (48.00%), Postives = 19/25 (76.00%), Query Frame = -2

Query:  273 SFITDCIKLDPDERIEFGDLLSHDW 347
            SFI  C+K +P++R+   +LLSH+W
Sbjct:  220 SFIRGCLKSNPEKRLPLDELLSHNW 244

[Close]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Match: pimr72 (Pim proto-oncogene, serine/threonine kinase, related 72 [Source:ZFIN;Acc:ZDB-GENE-091118-122])

HSP 1 Score: 44.669 bits (104), Expect = 2.402e-7
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  386 GYYHRDIKPKNIMVNEETLEVKLIDL--EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 607
            G  HRDIK +N+++N+ETLEVKLID    D+L        C    GT  +  PE      Y+ K + V ++G + Y
Sbjct:  135 GVLHRDIKLENLLINKETLEVKLIDFGCGDLLKTSAYTTFC----GTEDYCPPEFRSSGRYHGKAATVWSLGVLLY 206

HSP 2 Score: 28.8758 bits (63), Expect = 2.402e-7
Identity = 13/35 (37.14%), Postives = 19/35 (54.29%), Query Frame = -2

Query:  273 KINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 377
            K  LS+E  +  +  C++  P +RI  G L SH W
Sbjct:  229 KSGLSTECCQ-LVQSCLQRSPRKRIALGKLSSHRW 262

[Close]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Match: pimr74 (Pim proto-oncogene, serine/threonine kinase, related 74 [Source:ZFIN;Acc:ZDB-GENE-120215-19])

HSP 1 Score: 44.669 bits (104), Expect = 4.129e-7
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  386 GYYHRDIKPKNIMVNEETLEVKLIDL--EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 607
            G  HRDIK +N+++N+ETLEVKLID    D+L        C    GT  +  PE      Y+ K + V ++G + Y
Sbjct:  134 GVLHRDIKLENLLINKETLEVKLIDFGCGDLLKTSAYTTFC----GTEDYCPPEFRSSGRYHGKAATVWSLGVLLY 205

HSP 2 Score: 28.1054 bits (61), Expect = 4.129e-7
Identity = 13/35 (37.14%), Postives = 18/35 (51.43%), Query Frame = -2

Query:  273 KINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 377
            K  LS E  +  +  C++  P +RI  G L SH W
Sbjct:  228 KSGLSKECCQ-LVQSCLQRSPRKRIALGKLSSHRW 261

[Close]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Match: pimr73 (Pim proto-oncogene, serine/threonine kinase, related 73 [Source:ZFIN;Acc:ZDB-GENE-120215-17])

HSP 1 Score: 44.669 bits (104), Expect = 4.227e-7
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  386 GYYHRDIKPKNIMVNEETLEVKLIDL--EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 607
            G  HRDIK +N+++N+ETLEVKLID    D+L        C    GT  +  PE      Y+ K + V ++G + Y
Sbjct:  138 GVLHRDIKLENLLINKETLEVKLIDFGCGDLLKTSAYTTFC----GTEDYCPPEFRSSGRYHGKAATVWSLGVLLY 209

HSP 2 Score: 27.7202 bits (60), Expect = 4.227e-7
Identity = 13/35 (37.14%), Postives = 18/35 (51.43%), Query Frame = -2

Query:  273 KINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 377
            K  LS E  +  +  C++  P +RI  G L SH W
Sbjct:  232 KSGLSKECCQ-LVQSCLQRSPRKRIALGKLSSHRW 265

[Close]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Match: pimr70 (Pim proto-oncogene, serine/threonine kinase, related 70 [Source:ZFIN;Acc:ZDB-GENE-120215-28])

HSP 1 Score: 44.669 bits (104), Expect = 4.303e-7
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  386 GYYHRDIKPKNIMVNEETLEVKLIDL--EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 607
            G  HRDIK +N+++N+ETLEVKLID    D+L        C    GT  +  PE      Y+ K + V ++G + Y
Sbjct:  129 GVLHRDIKLENLLINKETLEVKLIDFGCGDLLKTSAYTTFC----GTEDYCPPEFRSSGRYHGKAATVWSLGVLLY 200

HSP 2 Score: 28.1054 bits (61), Expect = 4.303e-7
Identity = 13/35 (37.14%), Postives = 18/35 (51.43%), Query Frame = -2

Query:  273 KINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 377
            K  LS E  +  +  C++  P +RI  G L SH W
Sbjct:  223 KPGLSKECCQ-LVQSCLQRSPRKRIALGKLSSHRW 256

[Close]

BLAST of SMED30032788 vs. UniProt/SwissProt
Match: sp|U4PR86|MELK_CAEEL (Maternal embryonic leucine zipper kinase OS=Caenorhabditis elegans OX=6239 GN=pig-1 PE=1 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 1.767e-7
Identity = 42/156 (26.92%), Postives = 80/156 (51.28%), Query Frame = -3

Query:  377 ELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPY--ESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDL------EDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  L  LS ++ + +L  YI+  +   +VMEY  G   ++ DY ++     ES  +   + L+  +  +   GY HRD+KP+N+++ E+ L +KLID       E    D++++  C    G+  + +PE+   + Y   ++ V ++G + Y  L
Sbjct:   57 EMDALRNLS-HQNICRLYHYIETEDKFFIVMEYCSGG--EMFDYIVRKERLEESEARHFFRQLVSAIAFVHSQGYAHRDLKPENLLLTED-LHLKLIDFGLCAKTEKGRIDKHNLDTC---CGSPAYAAPELIQGLQYKGNEADVWSMGILLYTLL 205

HSP 2 Score: 29.261 bits (64), Expect = 1.767e-7
Identity = 11/33 (33.33%), Postives = 19/33 (57.58%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S L++  +   +++ P+ RI    LL HDW +H
Sbjct:  235 SPLSKQLLRAMLQVVPERRISVKKLLEHDWLNH 267

[Close]

BLAST of SMED30032788 vs. TrEMBL
Match: A0A2P1ERR9 (PIM-1-like protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 9.222e-63
Identity = 110/150 (73.33%), Postives = 130/150 (86.67%), Query Frame = -3

Query:  374 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK 823
            +EL FLE+LSQYEYVPKLI YIDENNWSTV MEY+ GDWMDL  Y ++F  E  +KTI++NLI IVYKMS+IGYYHRDIKP+NIMVN+ TLE+KLIDLED+L+D+ +IP C S LGTLGFQSPE +G VPYNLKQSLV NIGC+AYFC++
Sbjct:   12 KELFFLEELSQYEYVPKLIEYIDENNWSTVAMEYIGGDWMDLSYYLVEFEGEKLVKTIMRNLISIVYKMSDIGYYHRDIKPENIMVNQRTLEIKLIDLEDMLYDENEIPKCISELGTLGFQSPETYGKVPYNLKQSLVFNIGCLAYFCIE 161

HSP 2 Score: 62.3882 bits (150), Expect = 1.074e-7
Identity = 31/52 (59.62%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  273 FKTKLSLQHWMCSIFLSKINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 428
            F TK+  Q  +    L ++ +SS+LARSFIT+CIKLDP+ERIEF DLL HDW
Sbjct:  166 FDTKIETQKCL----LPEMKVSSKLARSFITECIKLDPNERIEFDDLLFHDW 213

[Close]

BLAST of SMED30032788 vs. TrEMBL
Match: A0A507BQU4 (Protein kinase domain-containing protein OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g04701 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.998e-8
Identity = 51/156 (32.69%), Postives = 82/156 (52.56%), Query Frame = -3

Query:  377 ELLFLEQLS-QYEYVPKLITYIDENNWST---VVMEYLDGDWMDLHDYT-MQFP-YESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTL--GTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E+  + QL+ Q    P ++ YI+   ++T   +VMEYL  DW+DL+DY  +  P  E   + I   ++  +  + ++GY H D+K +NIM+N +T EVK+ID          IP   ++L  GT  F +PE    +PY  +   V  +G + Y  L
Sbjct:  478 EIHLMRQLAIQQPPHPAIVKYIEHFEYNTRYIIVMEYLGEDWVDLYDYVEVHGPVREDHSREIFTQIVDAIEFLHKLGYSHNDVKDENIMINTKTREVKIIDFGSAT---PLIPGQTASLFFGTKKFAAPEAVQGLPYYPEAQEVWALGALLYVLL 630

[Close]

BLAST of SMED30032788 vs. TrEMBL
Match: A0A1Y2CTR7 (Kinase-like protein (Fragment) OS=Rhizoclosmatium globosum OX=329046 GN=BCR33DRAFT_656547 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.578e-7
Identity = 44/144 (30.56%), Postives = 74/144 (51.39%), Query Frame = -3

Query:  377 PKLITYIDE---NNWSTVVMEYLDGDWMDLHDYTMQFP--YESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVL----FDQYDIPICNSTLGTLGFQSPE-IFGDVPYNLKQSLVCNIGCVAYFCL 778
            P LI Y +    N    ++MEY+  +W+DL+DY  ++    E     I ++++  +  +  IGYYH DIK +N+M++ +T ++KLID          D     +C +  GT  F +PE + GD PYN +      +G + +  L
Sbjct:   73 PNLINYYEHFELNGKFMIIMEYMGENWIDLYDYIEEYGPVREDVSMEIFRSIVETLVFLHSIGYYHNDIKDENVMIHSKTRQIKLIDFGSATPVPGPDDKTPNLCENFYGTKKFAAPEAVLGD-PYNPEMQESWALGTLLFVLL 215

[Close]

BLAST of SMED30032788 vs. TrEMBL
Match: A0A1Y2BGF1 (Kinase-like protein (Fragment) OS=Rhizoclosmatium globosum OX=329046 GN=BCR33DRAFT_665162 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.709e-7
Identity = 44/144 (30.56%), Postives = 74/144 (51.39%), Query Frame = -3

Query:  377 PKLITYIDE---NNWSTVVMEYLDGDWMDLHDYTMQFP--YESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVL----FDQYDIPICNSTLGTLGFQSPE-IFGDVPYNLKQSLVCNIGCVAYFCL 778
            P LI Y +    N    ++MEY+  +W+DL+DY  ++    E     I ++++  +  +  IGYYH DIK +N+M++ +T ++KLID          D     +C +  GT  F +PE + GD PYN +      +G + +  L
Sbjct:   73 PNLINYYEHFELNGKFMIIMEYMGENWIDLYDYIEEYGPVREDVSMEIFRSIVETLVFLHSIGYYHNDIKDENVMIHSKTRQIKLIDFGSATPVPGPDDKTPNLCENFYGTKKFAAPEAVLGD-PYNPEMQESWALGTLLFVLL 215

[Close]

BLAST of SMED30032788 vs. TrEMBL
Match: A0A060T9H3 (ARAD1C44660p OS=Blastobotrys adeninivorans OX=409370 GN=GNLVRS02_ARAD1C44660g PE=3 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 6.366e-7
Identity = 44/152 (28.95%), Postives = 74/152 (48.68%), Query Frame = -3

Query:  377 ELLFLEQLSQ-YEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNI--MVNEETLEVKLIDLE-DVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL 820
            E++ L++LSQ ++ V  LI Y +  N   +V +Y  G  +    Y     YES    +I+ +   V  +   G  HRD+KP+N+  +  +E  E+ + D     + D+    +  +T GT  + +PEIF    ++ K   V  IG + YF L
Sbjct:   61 EIVVLKRLSQGHKNVLTLIDYFETANNLYLVTDYARGGELFDRIYNKGSFYESNAAAMIRQITAGVAYLHSHGIVHRDLKPENLLFLTPDEDSELMIADFGLSRIIDEEKFRMLTTTCGTPAYMAPEIFQKTGHH-KPVDVWAIGVITYFLL 211

HSP 2 Score: 35.039 bits (79), Expect = 6.366e-7
Identity = 15/33 (45.45%), Postives = 20/33 (60.61%), Query Frame = -2

Query:  264 SELARSFITDCIKLDPDERIEFGDLLSHDWQHH 362
            S+ AR FIT C+K+DP ER+     L H +  H
Sbjct:  246 SDTARDFITRCLKIDPSERMTAQQCLEHPFLAH 278

[Close]

BLAST of SMED30032788 vs. Ensembl Cavefish
Match: ENSAMXT00000009626.2 (serine/threonine-protein kinase pim-1-like [Source:NCBI gene;Acc:111194017])

HSP 1 Score: 47.7506 bits (112), Expect = 7.322e-7
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  386 EIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 613
            E G +HRDIKP N ++N +TLE+KLID      D   +   NS  GT  +  PE      Y+ K + V ++G + Y
Sbjct:  227 ERGVFHRDIKPGNFLINPQTLELKLIDFG--CADWVKLEGNNSYAGTRVYCPPEYLIKRRYHAKPATVWSLGVMLY 300

HSP 2 Score: 23.8682 bits (50), Expect = 7.322e-7
Identity = 7/16 (43.75%), Postives = 12/16 (75.00%), Query Frame = -2

Query:  273 DPDERIEFGDLLSHDW 320
            +P++R  FG +L+H W
Sbjct:  341 NPNKRPCFGQILNHSW 356

[Close]

BLAST of SMED30032788 vs. Ensembl Cavefish
Match: ENSAMXT00000030133.1 (serine/threonine-protein kinase pim-3-like [Source:NCBI gene;Acc:103028120])

HSP 1 Score: 48.521 bits (114), Expect = 2.612e-6
Identity = 47/151 (31.13%), Postives = 72/151 (47.68%), Query Frame = -3

Query:  386 ELLFLEQLSQ--YEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDY---TMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLE-DVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 820
            E+  +E++S+   + + +LI + DE     VVME      MDL D+      F  E   + I+   I  V +  + G  HRDIK +N ++N  T EVKL+D      F + ++   N  +GT  F  PE F    Y+   S V ++G   Y
Sbjct:  114 EVALIEKMSEPPVKSIIQLIQWFDEPERYIVVMELFR-PCMDLLDFMCSKRGFFTEKKARHIMVQAIKAVTECHKRGVLHRDIKLENFLINTNTFEVKLVDFGCGDFFTEREL---NHYIGTEEFCPPEFFLSRSYHAAPSTVWSLGVFLY 260

[Close]

BLAST of SMED30032788 vs. Ensembl Cavefish
Match: ENSAMXT00000043607.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:33833945:33836628:-1 gene:ENSAMXG00000042906.1 transcript:ENSAMXT00000043607.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.669 bits (104), Expect = 4.095e-6
Identity = 28/78 (35.90%), Postives = 42/78 (53.85%), Query Frame = -3

Query:  386 MSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAY 619
            M E    HRD+K  NI+V  +   VK+ D       +    +CNS +GTL + SPE+  +  Y+ K+S V ++G V Y
Sbjct:  152 MHEQDIVHRDLKCDNILVTADG-NVKIADFG---LSRVTKGLCNSMVGTLWYVSPEVIREKKYDAKKSDVWSLGIVLY 225

HSP 2 Score: 24.2534 bits (51), Expect = 4.095e-6
Identity = 10/29 (34.48%), Postives = 14/29 (48.28%), Query Frame = -2

Query:  273 ELARSFITDCIKLDPDERIEFGDLLSHDW 359
            E  R+ I D ++LDP  R     +  H W
Sbjct:  259 ESCRALILDMLQLDPSARPTVKKVSEHPW 287

[Close]

BLAST of SMED30032788 vs. Ensembl Cavefish
Match: ENSAMXT00000040711.1 (serine/threonine-protein kinase pim-1-like [Source:NCBI gene;Acc:103035777])

HSP 1 Score: 43.1282 bits (100), Expect = 5.108e-6
Identity = 27/76 (35.53%), Postives = 40/76 (52.63%), Query Frame = -3

Query:  398 GYYHRDIKPKNIMVNEETLEVKLID------LEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIG 607
            G  HRDIKP+N ++N  TLE+KLID      L+   ++ Y         GT  +  PE+F    Y+ K + V ++G
Sbjct:  178 GVLHRDIKPENFLINPNTLELKLIDFGCGDWLKKYGYNLYS--------GTFEYVPPELFRKRRYHSKTATVWSLG 245

HSP 2 Score: 25.409 bits (54), Expect = 5.108e-6
Identity = 9/26 (34.62%), Postives = 14/26 (53.85%), Query Frame = -2

Query:  267 FITDCIKLDPDERIEFGDLLSHDWQH 344
             I  C++  P +R    ++L HDW H
Sbjct:  282 LIRQCLQYIPAKRPTIREILKHDWLH 307

[Close]

BLAST of SMED30032788 vs. Planmine SMEST
Match: SMESG000061865.1 (SMESG000061865.1)

HSP 1 Score: 278.1 bits (710), Expect = 1.121e-94
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = -3

Query:  374 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK 823
            RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPIKTIIKNLIIIVYKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL+
Sbjct:   66 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPIKTIIKNLIIIVYKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLE 215

[Close]

BLAST of SMED30032788 vs. Planmine SMEST
Match: SMESG000061880.1 (SMESG000061880.1)

HSP 1 Score: 278.87 bits (712), Expect = 9.548e-94
Identity = 151/156 (96.79%), Postives = 152/156 (97.44%), Query Frame = -3

Query:  356 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLKSIFHQN 823
            RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPIKTIIKNLIIIVYKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCL+  F  N
Sbjct:   66 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPIKTIIKNLIIIVYKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLEFQFPFN 221

HSP 2 Score: 69.707 bits (169), Expect = 1.398e-13
Identity = 35/54 (64.81%), Postives = 40/54 (74.07%), Query Frame = -2

Query:  273 LQFKTKLSLQHWMCSIFLSKINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 434
             QF     ++   C+  L KINLSS+LARSFITDCIK DPDERIEFGDLL H+W
Sbjct:  216 FQFPFNSKIETKKCN--LPKINLSSDLARSFITDCIKPDPDERIEFGDLLFHNW 267

[Close]

BLAST of SMED30032788 vs. Planmine SMEST
Match: SMESG000032312.1 (SMESG000032312.1)

HSP 1 Score: 254.988 bits (650), Expect = 6.611e-85
Identity = 136/150 (90.67%), Postives = 141/150 (94.00%), Query Frame = -3

Query:  374 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK 823
            RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYL GDWMDL  +TM FP E PIKTIIKNLIIIVY+MSEIGYYHRDIKP+NIMVN+ETLEVKLIDLED LFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVC  GCVAYFCL+
Sbjct:   66 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLGGDWMDLSKFTMAFPIEKPIKTIIKNLIIIVYEMSEIGYYHRDIKPENIMVNKETLEVKLIDLEDALFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCTFGCVAYFCLE 215

HSP 2 Score: 78.1814 bits (191), Expect = 6.611e-85
Identity = 36/40 (90.00%), Postives = 38/40 (95.00%), Query Frame = -2

Query:  264 LSKINLSSELARSFITDCIKLDPDERIEFGDLLSHDWQHH 383
            L KINLSSELARSFITDCIKLDPDERIEFGDLLSHDW ++
Sbjct:  231 LPKINLSSELARSFITDCIKLDPDERIEFGDLLSHDWLYN 270

[Close]

BLAST of SMED30032788 vs. Planmine SMEST
Match: SMESG000000218.1 (SMESG000000218.1)

HSP 1 Score: 228.409 bits (581), Expect = 8.593e-74
Identity = 119/153 (77.78%), Postives = 138/153 (90.20%), Query Frame = -3

Query:  365 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLKSIF 823
            +ELLFLEQLSQYEYVPKLI YIDENNWSTVVMEYL+GDWM+L ++TM+F  E  IK I+ NLI+IVY+MS++GYYHRDIKP+NIM+N ET EVKLIDLEDVL++  D+PICNSTLGT+G+QSPEIFGDVPYNLKQSLV NIGCVAYFCL+  F
Sbjct:   85 KELLFLEQLSQYEYVPKLIKYIDENNWSTVVMEYLNGDWMNLGEFTMRFADEKLIKIIMINLILIVYEMSDLGYYHRDIKPENIMINRETFEVKLIDLEDVLYNIDDMPICNSTLGTIGYQSPEIFGDVPYNLKQSLVFNIGCVAYFCLEFQF 237

HSP 2 Score: 63.1586 bits (152), Expect = 2.490e-11
Identity = 27/37 (72.97%), Postives = 32/37 (86.49%), Query Frame = -2

Query:  273 LSKINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 383
            L K+ +SSELA+SFI +CIKLDPDERIEFG+ LSH W
Sbjct:  250 LPKMKVSSELAQSFIANCIKLDPDERIEFGEFLSHQW 286

[Close]

BLAST of SMED30032788 vs. Planmine SMEST
Match: SMESG000061809.1 (SMESG000061809.1)

HSP 1 Score: 211.075 bits (536), Expect = 3.893e-67
Identity = 111/150 (74.00%), Postives = 132/150 (88.00%), Query Frame = -3

Query:  374 RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFPYESPXXXXXXXXXXXXXKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLEDVLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK 823
            +EL FLE+LSQYEYVPKLI YIDENNWSTV MEY+ GDWMDL  Y ++F  E  +KTI++NLI IVYKMS+IGYYHRDIKP+NIMVN++TLE+KLIDLED+L+D+ +IPIC S LGTLGFQSPE +G VPYNLKQSLV NIGC+AYFC++
Sbjct:   66 KELFFLEELSQYEYVPKLIEYIDENNWSTVAMEYIGGDWMDLSYYLVEFEGEKLVKTIMRNLISIVYKMSDIGYYHRDIKPENIMVNQKTLEIKLIDLEDMLYDENEIPICISELGTLGFQSPETYGKVPYNLKQSLVFNIGCLAYFCIE 215

HSP 2 Score: 63.929 bits (154), Expect = 1.272e-11
Identity = 32/52 (61.54%), Postives = 39/52 (75.00%), Query Frame = -2

Query:  273 FKTKLSLQHWMCSIFLSKINLSSELARSFITDCIKLDPDERIEFGDLLSHDW 428
            F TK+  Q  +    L ++ +SS+LARSFIT+CIKLDPDERIEF DLL HDW
Sbjct:  220 FDTKIETQKCL----LPEMKVSSKLARSFITECIKLDPDERIEFDDLLFHDW 267

The following BLAST results are available for this feature:

BLAST of SMED30032788 vs. Ensembl Human
BLAST of SMED30032788 vs. Ensembl Celegans
BLAST of SMED30032788 vs. Ensembl Fly
BLAST of SMED30032788 vs. Ensembl Zebrafish
BLAST of SMED30032788 vs. Ensembl Xenopus
BLAST of SMED30032788 vs. Ensembl Mouse
BLAST of SMED30032788 vs. UniProt/SwissProt
BLAST of SMED30032788 vs. TrEMBL
BLAST of SMED30032788 vs. Ensembl Cavefish
BLAST of SMED30032788 vs. Ensembl Sea Lamprey
BLAST of SMED30032788 vs. Ensembl Yeast
BLAST of SMED30032788 vs. Ensembl Nematostella
BLAST of SMED30032788 vs. Ensembl Medaka
BLAST of SMED30032788 vs. Planmine SMEST

BLAST of SMED30032788 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pig-1	1.747e-8	26.92	Maternal embryonic leucine zipper kinase [Source:...	[more]
pig-1	1.761e-8	26.92	Maternal embryonic leucine zipper kinase [Source:...	[more]
pig-1	1.775e-8	26.92	Maternal embryonic leucine zipper kinase [Source:...	[more]
pig-1	1.863e-8	26.92	Maternal embryonic leucine zipper kinase [Source:...	[more]
nekl-2	1.008e-6	29.73	Serine/threonine-protein kinase nekl-2 [Source:Un...	[more]

BLAST of SMED30032788 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
fu	9.490e-6	28.74	gene:FBgn0001079 transcript:FBtr0074602	[more]

BLAST of SMED30032788 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pimr68	2.268e-8	35.63	Pim proto-oncogene, serine/threonine kinase, relat...	[more]
pimr72	2.402e-7	38.16	Pim proto-oncogene, serine/threonine kinase, relat...	[more]
pimr74	4.129e-7	38.16	Pim proto-oncogene, serine/threonine kinase, relat...	[more]
pimr73	4.227e-7	38.16	Pim proto-oncogene, serine/threonine kinase, relat...	[more]
pimr70	4.303e-7	38.16	Pim proto-oncogene, serine/threonine kinase, relat...	[more]

BLAST of SMED30032788 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1


Match Name	E-value	Identity	Description
sp\|U4PR86\|MELK_CAEEL	1.767e-7	26.92	Maternal embryonic leucine zipper kinase OS=Caenor...	[more]

BLAST of SMED30032788 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2P1ERR9	9.222e-63	73.33	PIM-1-like protein OS=Schmidtea mediterranea OX=79...	[more]
A0A507BQU4	2.998e-8	32.69	Protein kinase domain-containing protein OS=Synchy...	[more]
A0A1Y2CTR7	4.578e-7	30.56	Kinase-like protein (Fragment) OS=Rhizoclosmatium ...	[more]
A0A1Y2BGF1	4.709e-7	30.56	Kinase-like protein (Fragment) OS=Rhizoclosmatium ...	[more]
A0A060T9H3	6.366e-7	28.95	ARAD1C44660p OS=Blastobotrys adeninivorans OX=4093...	[more]

BLAST of SMED30032788 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
ENSAMXT00000009626.2	7.322e-7	38.16	serine/threonine-protein kinase pim-1-like [Source...	[more]
ENSAMXT00000030133.1	2.612e-6	31.13	serine/threonine-protein kinase pim-3-like [Source...	[more]
ENSAMXT00000043607.1	4.095e-6	35.90	pep primary_assembly:Astyanax_mexicanus-2.0:20:338...	[more]
ENSAMXT00000040711.1	5.108e-6	35.53	serine/threonine-protein kinase pim-1-like [Source...	[more]

BLAST of SMED30032788 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032788 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000061865.1	1.121e-94	99.33	SMESG000061865.1	[more]
SMESG000061880.1	9.548e-94	96.79	SMESG000061880.1	[more]
SMESG000032312.1	6.611e-85	90.67	SMESG000032312.1	[more]
SMESG000000218.1	8.593e-74	77.78	SMESG000000218.1	[more]
SMESG000061809.1	3.893e-67	74.00	SMESG000061809.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30032788 ID=SMED30032788|Name=SMED30032788|organism=Schmidtea mediterranea sexual|type=transcript|length=825bp
ATTTGATTTAAAAATGTCAATGGCGATGGAAATTGTCGAAGTACCAACTT
ATATTTCTGTCGACGAGATGAATACAAGTAAAACTGTAGATGGCGGAAAC
AAGAAAAAAGTGAAAATGAAACAGCGTAATTGGGAAGAGGAAATTATATT
TAACACAAATGTGATCGATGAAAACCAGTGGAGTTATCATTATACAAACT
CAACAGATGCAAGAAAAAAGAAGTTTTTTCGATGTAATAAAGTCAAAGCT
CGTGGTAAACAATGTGATGCTGCCAATCATGAGAAAGAAGGTCACCAAAT
TCAATCCGTTCATCGGGATCTAGTTTAATGCAATCCGTGATAAATGATCG
AGCTAGTTCTGATGAAAGATTGATTTTAGACAAAAATATGCTACACATCC
AATGTTGCAAACTAAGCTTTGTTTTAAATTGTAAGGAACATCACCAAATA
TTTCAGGGCTTTGGAAACCAAGTGTTCCTAATGTTGAATTACAAATCGGA
ATATCATATTGATCAAAAAGTACATCTTCTAAATCAATAAGTTTCACTTC
AAGCGTTTCTTCATTGACCATAATATTTTTTGGTTTGATATCTCTGTGAT
AATATCCGATTTCTGACATCTTATATACAATTATAATTAAATTTTTTATT
ATAGTTTTGATTGGGCTTTCATATGGAAATTGCATTGTATAATCATGCAA
ATCCATCCAATCACCATCAAGATATTCCATAACAACTGTTGACCAATTAT
TTTCATCAATATATGTTATTAATTTCGGAACATATTCATATTGAGATAAC
TGTTCTAAAAATAACAATTCTCTTG

protein sequence of SMED30032788-orf-1

>SMED30032788-orf-1 ID=SMED30032788-orf-1|Name=SMED30032788-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=157bp
RELLFLEQLSQYEYVPKLITYIDENNWSTVVMEYLDGDWMDLHDYTMQFP
YESPIKTIIKNLIIIVYKMSEIGYYHRDIKPKNIMVNEETLEVKLIDLED
VLFDQYDIPICNSTLGTLGFQSPEIFGDVPYNLKQSLVCNIGCVAYFCLK
SIFHQN*

protein sequence of SMED30032788-orf-2

>SMED30032788-orf-2 ID=SMED30032788-orf-2|Name=SMED30032788-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
FDLKMSMAMEIVEVPTYISVDEMNTSKTVDGGNKKKVKMKQRNWEEEIIF
NTNVIDENQWSYHYTNSTDARKKKFFRCNKVKARGKQCDAANHEKEGHQI
QSVHRDLV*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000429	neoblast
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **biological process**
Term	Definition
GO:0006468	protein phosphorylation

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 87..108

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SMED30032788

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

blazegraph-runner