SMED30032725

Overview
NameSMED30032725
Smed IDSMED30032725
Length (bp)4339
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032725 (SMED30032725) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032725 (SMED30032725) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032725 (SMED30032725) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032725 (SMED30032725) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032725

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30032725SMESG000011874.1 dd_Smed_v4_14669_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032725SMESG000011874.1 dd_Smed_v4_14669_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30032725SMESG000011874.1 dd_Smed_v4_14669_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30032725SMESG000011874.1 dd_Smed_v4_14669_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30032725 vs. Planmine SMEST
Match: SMESG000011874.1 (SMESG000011874.1)

HSP 1 Score: 198.749 bits (504), Expect = 1.259e-86
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 2 
Query:  695 DKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVXXXXXXXXXXXXXXLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN 1042
            DKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVSAHSMHNMHHSWNSLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN
Sbjct:   87 DKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVSAHSMHNMHHSWNSLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN 202

HSP 2 Score: 142.895 bits (359), Expect = 1.259e-86
Identity = 86/88 (97.73%), Postives = 86/88 (97.73%), Query Frame = 3 
Query:  384 MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGXXXXXXXXXXXXXXXPQYGIYTGFSLLVLLNFENFYKCT*SK 647
            MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGNKQMLMNRNLNLNYNPQYGIYTGFSLLVLLNFENFYKCT  K
Sbjct:    1 MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGNKQMLMNRNLNLNYNPQYGIYTGFSLLVLLNFENFYKCTYDK 88
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BLAST of SMED30032725 vs. Planmine SMEST
Match: SMESG000011874.1 (SMESG000011874.1)

HSP 1 Score: 260.381 bits (664), Expect = 4.795e-79
Identity = 147/158 (93.04%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:  569 PIWYLYWIFFVGIIKFREFLQVYLVKRKNFCGKRTLESNAYSDKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVXXXXXXXXXXXXXXLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN 1042
            P + +Y  F   ++KFREFLQVYLVKRKNFCGKRTLESNAYSDKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVSAHSMHNMHHSWNSLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN
Sbjct:   62 PQYGIYTGF--SLLKFREFLQVYLVKRKNFCGKRTLESNAYSDKGLTTSNEAVQLIVSSKPSDLQLIRTSTESNESLELLQKSNSSKYNIDHLKPFTVSAHSMHNMHHSWNSLSVSNYNCTLSSDNGIRPKDFDSLSIRTAVEFPNSNAFSSNVTILN 217

HSP 2 Score: 116.701 bits (291), Expect = 4.463e-29
Identity = 75/82 (91.46%), Postives = 75/82 (91.46%), Query Frame = 3 
Query:  384 MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGXXXXXXXXXXXXXXXPQYGIYTGFSLLVLLNFENFY 629
            MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGNKQMLMNRNLNLNYNPQYGIYTGFSLL    F   Y
Sbjct:    1 MKKPNETSINNNQTQIFSEQYYRELRQKYGVELSGLQQGFYHVIYGNKQMLMNRNLNLNYNPQYGIYTGFSLLKFREFLQVY 82
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BLAST of SMED30032725 vs. Planmine SMEST
Match: SMESG000039041.1 (SMESG000039041.1)

HSP 1 Score: 254.988 bits (650), Expect = 1.317e-75
Identity = 128/185 (69.19%), Postives = 148/185 (80.00%), Query Frame = -1 
Query: 1958 LDNSWIDASRITVRFPAHVANRYRQAMWDAGYTVSQVKESRFGTQQNSEEVDLPFVAIKWEPGRNPAREIRGQAEFLQARLEEHQRQTNAQVERAKRYLAEVKEDIAAGRIPSTD*YAPPYNTSYNLNDHRLDARSTAINVVVSRKILPRDMRKEENRPDSHREHPYAREPPRQDSNRYRREPNN 2512
            LD+SWID+SRITVRFPAH ANR RQA+WDAG+TVSQ KESRFG Q+ SEE+ LPFVAIK EPGRN AREIRG AEFL A LEEHQRQT+A VE A+RYLA V +DIA GRI STD YAP YNTS+NL+D RL+  S  INVVV  +ILPR+MR E+ + D HREHPYAR  PR+D+++    P N
Sbjct:   15 LDDSWIDSSRITVRFPAHAANRCRQALWDAGFTVSQAKESRFGQQRPSEEIKLPFVAIKREPGRNSAREIRGHAEFLIAPLEEHQRQTDAHVESARRYLANVNDDIAKGRILSTDGYAPSYNTSFNLDDRRLNTNSKTINVVVPWRILPRNMRTEKEQIDEHREHPYARINPRRDNHKRYYGPYN 199
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BLAST of SMED30032725 vs. Planmine SMEST
Match: SMESG000032540.1 (SMESG000032540.1)

HSP 1 Score: 225.713 bits (574), Expect = 2.160e-67
Identity = 113/186 (60.75%), Postives = 144/186 (77.42%), Query Frame = -1 
Query: 1958 FLDNSWIDASRITVRFPAHVANRYRQAMWDAGYTVSQVKESRFGTQQNSEEVDLPFVAIKWEPGRNPAREIRGQAEFLQARLEEHQRQTNAQVERAKRYLAEVKEDIAAGRIPSTD*YAPPYNTSYNLNDHRLDARSTAINVVVSRKILPRDMRKEENRPDSHREHPYAREPPRQDSNRYRREPNN 2515
            FL+NSWI ASR+T+RFPAH A R RQAMWDAG+++S V+ESR G++ N+++VDLPFVAIK EPGRNPAREIRGQAE+L ARLEEH+RQT+ +V++A+R+L E+  DIAAG IPSTD YAPPYNT +NL+D RL+  S+A+ VVV R+ L R   +     + H +HPYARE P +  N  R E  N
Sbjct:    4 FLENSWIYASRVTLRFPAHSAVRCRQAMWDAGFSISPVRESRMGSRSNTDDVDLPFVAIKREPGRNPAREIRGQAEYLLARLEEHKRQTDHEVDKARRHLEEINWDIAAGIIPSTDGYAPPYNTPFNLDDPRLNRSSSAMKVVVPRETLIRGWGEANEPANEHHDHPYARERPSRRDNSKRFECLN 189
[Close]
BLAST of SMED30032725 vs. Planmine SMEST
Match: SMESG000042239.1 (SMESG000042239.1)

HSP 1 Score: 219.164 bits (557), Expect = 6.193e-65
Identity = 107/146 (73.29%), Postives = 120/146 (82.19%), Query Frame = -1 
Query: 2066 SWIDASRITVRFPAHVANRYRQAMWDAGYTVSQVKESRFGTQQNSEEVDLPFVAIKWEPGRNPAREIRGQAEFLQARLEEHQRQTNAQVERAKRYLAEVKEDIAAGRIPSTD*YAPPYNTSYNLNDHRLDARSTAINVVVSRKILP 2503
            SWID SR+TVRFPAH ANR  QAM DAG+TVSQV+ESR GT Q  EEV+L FVAIK EPGRNPAREIRGQAEFL A LEEHQRQT+ QV+RA+RYLA V EDIAAGRI  TD YAPPYNTS+NL+D RL+ ++ AINVVV  +  P
Sbjct:   54 SWIDESRVTVRFPAHAANRCLQAMCDAGFTVSQVRESRSGTPQQMEEVELRFVAIKREPGRNPAREIRGQAEFLLASLEEHQRQTDIQVDRARRYLASVNEDIAAGRIACTDCYAPPYNTSFNLDDERLNTKAVAINVVVRGRSFP 199
The following BLAST results are available for this feature:
BLAST of SMED30032725 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032725 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000011874.11.259e-86100.00SMESG000011874.1[more]
SMESG000011874.14.795e-7993.04SMESG000011874.1[more]
SMESG000039041.11.317e-7569.19SMESG000039041.1[more]
SMESG000032540.12.160e-6760.75SMESG000032540.1[more]
SMESG000042239.16.193e-6573.29SMESG000042239.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032725 ID=SMED30032725|Name=SMED30032725|organism=Schmidtea mediterranea sexual|type=transcript|length=4339bp
TTCATTCATTAACATTCAATTTCACGAATTTACTCTTTTTCTTGTTAAAG
TGTAACGAATTTTTTCCCTCTTTAATCTTTAAGTATTTTCGAAAATACAG
CTAAAATCATTATCGAAAAAAATTATCATCTTTCTCAGTTTTTTAAATAT
CAAACTCTTCATGTCCACTTGTAATTCACAAGCGTAAACAGTTAATAGCA
TTTCTCTCCCAATTACACACCGACAATACACACACTGGCAGTGAATTTCC
ATTGACAGAAATAAAATCTAGTCACGTGCAATCACAACTTGCTGAAGAAA
ACGTCATTAAAAATCAAATTTTATGAATGTGTAAATTTACTTCGAAATTT
TATCGATTTTACTAGCCATTTTTTTTACCGATAATGAAAAAGCCGAATGA
AACGTCGATAAATAATAATCAAACCCAGATATTCAGTGAACAATATTATC
GGGAATTACGTCAGAAATATGGAGTCGAATTGTCTGGATTGCAACAAGGA
TTTTATCATGTTATCTATGGAAATAAACAAATGCTGATGAATAGAAATTT
GAATCTCAATTATAATCCCCAATATGGTATTTATACTGGATTTTCTTTGT
TGGTATTATTAAATTTCGAGAATTTCTACAAGTGTACCTAGTCAAAAGAA
AAAATTTTTGCGGAAAAAGAACTTTGGAATCAAATGCTTATAGTGACAAA
GGTTTAACGACATCGAATGAAGCAGTTCAATTGATTGTTTCATCGAAACC
TTCGGATTTACAACTCATACGAACATCTACAGAATCTAACGAATCTTTGG
AATTACTTCAGAAATCTAACAGTTCTAAGTATAACATTGACCATTTGAAA
CCTTTTACAGTTTCTGCTCATTCTATGCACAACATGCATCATTCCTGGAA
TAGTCTATCAGTCAGCAATTACAATTGCACCTTATCATCCGATAACGGTA
TCCGGCCAAAAGATTTTGATTCTCTCTCAATCAGAACTGCTGTCGAATTT
CCTAACTCAAATGCCTTTTCCTCGAATGTAACAATTTTGAATTAAATATT
TTTTACAGATCATCTGCTATTAAATAATTAATTAAAAAAAAATTTAATTA
ATCGATTGAATCATTTCTAATCGGATGTCGATTGAGTCAGTTGTAATTAT
TTTCTACCTCTTTTAACTATTTTCTGTGAATTTTATATTGAGTGAATTTT
TTCTTTTTCTAAAAACAATTTAATTTTATTGAATATAATTGAAAACGAAT
TAATAACAATGCGTCTATGCATTACTATTATTACATTTTCAACCCAATAT
AAAGGTGTTGAATTGTAATTATTGTTCGACAGGATAAAGCAATAAATCTT
ATTCAGCCTGTTCTCGATCAGAATAATAACGTTTCATTTTTATCACAAAT
CACAATCGTTCAAATGTACAACAATCTTTGCTTGCCAATAAGGACTGTCG
ATTCTAAGGAAAATTTAAATAGAAATTTCTCTCACTACTTTCAGCACATT
TTTACTCTTTCTACTACCATTATAGGGAATTGGATAAACTTTAAATTCCT
TTCATAGGACTCTATTCTCACCCTTCTGTGCAGCGGTCATCTTTTGATAT
AGTGCTTTGACTTTTCTCGGTTCCACCCAGGTGACCTTAATCTGCTGCAC
TTCTTTTGTCAGTTGTAAGATTTGATCGTATAATTTCTTGTTCGTTCTCC
GTGCGTTGTCCAACTCGCGCTGTAGAATCCGACATGATTCCATATCTACA
AAAAGGGAATTATCATTAAACCGTACAACTCTTCACAAATATTATTTATT
TCATTTATACTCTTGAGTCAGAGGATTTTAAAGTAGCGATTCACTAGGAT
CTTAAATTATAAATTACAGGAATTGATATCGATTCACTAGGGGCTTGAGT
TTTAAATTATAAGAATTGGTAACGAGACTACATATTTTATTTTCTATAAC
AAACACTATTATTCGGCTCTCGGCGATATCTGTTACTGTCTTGCCTTGGA
GGTTCTCGAGCATAAGGATGCTCGCGGTGGCTATCGGGTCGGTTTTCTTC
TTTTCTCATGTCACGGGGAAGGATCTTCCGTGAGACTACCACATTGATTG
CCGTAGACCTCGCGTCTAGCCTATGGTCGTTGAGGTTATATGAGGTATTG
TACGGGGGAGCGTATTAGTCTGTACTGGGAATTCTTCCTGCCGCAATATC
CTCTTTAACTTCAGCCAGATAACGCTTGGCTCTCTCCACTTGTGCATTGG
TCTGACGCTGATGCTCCTCGAGGCGAGCCTGGAGAAATTCGGCTTGTCCC
CGGATTTCGCGGGCCGGATTGCGTCCAGGTTCCCACTTTATAGCGACGAA
CGGGAGATCGACCTCCTCGGAATTCTGTTGCGTGCCGAATCTAGATTCTT
TTACCTGGGAGACGGTGTATCCTGCGTCCCACATGGCTTGGCGATATCTG
TTAGCCACATGTGCGGGGAATCGCACAGTGATTCTGGATGCATCGATCCA
CGAATTATCTAGAAAATCTTAATGATTAACCAGTTCGTAAACAACTTAAT
TTTATTAATCTGATTAGCTATGGATTGCCTAAACGATTATTGTGCACTTT
GTTGGGTCGGACACGTTATGTTGTTTTGGTCTAGTTCGTTGTGGTGATTA
TTCGAACTTTATCTTACTCTGATAATTATTTAACTTTGTAATTCAAATTT
TCCCGATTTCGCATTCCATTTTACTTAGATTTTGTAATTTGCATCGTCAA
ATTTGCGAAATTTAATTTATCGAACATCTTGTAATTTACTTTGCTTTCTT
ATATGTTCTGGTATAGCAATTCATAAATTACAGGATTGAAACTGGCTCTA
GTTGGCTTCAATTTGTCAGCGTCTAACTTTAGAGACGGGTGGCCTCTATG
ATCGTTTTCGATGTGGCAACTGTTTTAGAACTACGAAGGATCCCCTTTTT
CCTACATAGTTTTGGTTATAACCTCCACTGAGGGTTGTGCTACCGGTTTT
CATTATTGTGACTGTTACCTAGACCTATTGCTTGGGACTACTACATCCGA
TCTCATGATGTGGTTGGCTCATTCGTGTTGAACTCATCCCGTCTCAATTC
GCGGGCAATGAGAACCTTGATGACGAAGTAATTTCTAGTTTGGCCAACGT
TCGTATGCTTTATTATCATCTAGACTAAATAAAAACACGTGAATACTGAC
GCGGATAATTGATTCTAATAGACAACTATTGATTAGTAGGCGACTAGGCG
GTTAAAGATATGCGAGCATTCCATGTAAATAATTCATTGGTGAATGAAAC
ATATTTGTTAAAAACAAGACATATCCATTAATCATTGTATCTGGTTTTGA
GGGGCTTGCGTTCTGATTGGTAAATTTGTGTTGGGAATCCGTATCGTCTA
GTGATCTAGGCCTCGGACTTATGGTAAAACATTTAGAAAAGAAAATGAAA
ATTACCATCTCGGTGGTTAGTTCTTCGTTCCATTGTGATTTCGTCGTTCT
CGGTTTCTCGAAACCTGGCTGGGGCTTCACTTATCAACTGTCTGGAATTG
GATGGCATAATATGATCAGCGACTACGAGAAAAAATTGACGGTGTCCCGT
CGACAAGCGGAATTTATATGTGTATGGGTGCTTTTCTATGTCAGGCCGGT
ATGTGGTATGTACATCAAGATTGGCTGCACCCTGTTTCTGTTACAAGTGA
TGTGCGGTTTCACTTTGGCGGGAACGAGTGGGTGGAATTCGGTCTGCCAA
TGGTTAGATATTCTGTCGATAAATATCAGGGCAAATTATCATCGATCTAG
TTAGTTATCTAAATGTCGGTTTAGTTTCTTTATTAATTATTGTCTGATTT
ATCTGTAACATTATTTAATGGCAACTATTTTGGTGGTTCGTTGGTCATCA
TGGCTACCTTCTCGCAATAGTATTAGTCGATTTGAGTTAGACTATTTTAT
CGATGGTCATACTTCATCTCTTCGGTAAGTCGGGGTGTGCCCTAATCGTA
CTGATTATCCGAATTACCTCAGTTGGACGAACTCCCATTACATATCAGTT
ATGGATCTCTCTTATGGTGGCTTTTAGTGGGCACCAACGATTATATTATG
GAGTGTGAAGATCTGACGTAATATAATTTTCGGACAAATATTCATTACGA
AATTCTTTTATTTTCGTTCTTACTTGATACTCTTTATTCATGTATATTGA
TTTATAAATCTAGTAAATGTATCTGTTCCTGTTGTTTTTGACGTCAGCTT
TTTGGGTAAGAAATTTTAAATTGAGAGCTTGCGTTTCCATATCATGCTAT
CTTATGGTTTTAATTCTAATATTGTTAACTATTCATAAG
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protein sequence of SMED30032725-orf-1

>SMED30032725-orf-1 ID=SMED30032725-orf-1|Name=SMED30032725-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MVKHLEKKMKITISVVSSSFHCDFVVLGFSKPGWGFTYQLSGIGWHNMIS
DYEKKLTVSRRQAEFICVWVLFYVRPVCGMYIKIGCTLFLLQVMCGFTLA
GTSGWNSVCQWLDILSINIRANYHRSS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
Vocabulary: INTERPRO
TermDefinition
IPR011528NERD
IPR005514DUF316