Methylmalonate-semialdehyde dehydrogenase

Overview
NameMethylmalonate-semialdehyde dehydrogenase
Smed IDSMED30032682
Length (bp)1758
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Methylmalonate-semialdehyde dehydrogenase (SMED30032682) t-SNE clustered cells

Violin plots show distribution of expression levels for Methylmalonate-semialdehyde dehydrogenase (SMED30032682) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Methylmalonate-semialdehyde dehydrogenase (SMED30032682) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Methylmalonate-semialdehyde dehydrogenase (SMED30032682) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032682

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032682h1SMcG0013054 Contig47113newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032682h1SMcG0013054 Contig47113uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30032682h1SMcG0013054 dd_Smed_v4_2050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Match: ALDH6A1 (aldehyde dehydrogenase 6 family member A1 [Source:HGNC Symbol;Acc:HGNC:7179])

HSP 1 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 341/499 (68.34%), Postives = 419/499 (83.97%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI G FV+S++ +WID+ NPATN+VI RVP+ TK EM+ A+ SC  AF +W+  SVL+RQQ + + Q+LIK +LK+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L +GETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQH+AVNFICDHP I+AISFVG +KAG +I+ERGS++GKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++  AKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV P+M CYKEEIFGPVL+ LE +TLD+AI+++NNNPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAFPAWADTSVLSRQQVLLRYQQLIKENLKEIAKLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMMGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEYIFERGSRHGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 533          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Match: ALDH6A1 (aldehyde dehydrogenase 6 family member A1 [Source:HGNC Symbol;Acc:HGNC:7179])

HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0
Identity = 336/499 (67.33%), Postives = 410/499 (82.16%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI G FV+S++ +WID+ NPATN+VI RVP+ TK EM+ A+ SC  AF +W+  SVL+RQQ             K+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L +GETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQH+AVNFICDHP I+AISFVG +KAG +I+ERGS++GKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++  AKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV P+M CYKEEIFGPVL+ LE +TLD+AI+++NNNPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAFPAWADTSVLSRQQ-------------KEIAKLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMMGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEYIFERGSRHGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 520          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Match: ALDH6A1 (aldehyde dehydrogenase 6 family member A1 [Source:HGNC Symbol;Acc:HGNC:7179])

HSP 1 Score: 387.111 bits (993), Expect = 5.125e-132
Identity = 180/252 (71.43%), Postives = 215/252 (85.32%), Query Frame = 3
Query:  864 IGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++  AKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV P+M CYKEEIFGPVL+ LE +TLD+AI+++NNNPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:    1 MGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 250          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Match: ALDH1A1 (aldehyde dehydrogenase 1 family member A1 [Source:HGNC Symbol;Acc:HGNC:402])

HSP 1 Score: 231.106 bits (588), Expect = 2.590e-68
Identity = 160/489 (32.72%), Postives = 249/489 (50.92%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN----VPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN ++ DS + +   + NPAT + + +V +  K ++++AV++  +AF+    W       R + ++KL +LI+RD   +A   +   GK   +A  + + G ++ + +C    +   G T+P I  +    +  EP+GV   I P+NFP ++ +W    +L CGNT+++KP+E+ P   + +  L  EAGFPPG VN++ G    A   I  H  I  ++F G  + G+ I E  G  N KRV   +G K+  +++ DA  ++ +       F   GQ C+A S  +FV E+    ++   + RAK   +   + PG   GP I K   ++I  LIESG KEGAK+   G      GY    FV PT+ +NV   M   KEEIFGPV   ++  +LDD IK  NN  YG    +FT     A   +  +  G V VN    V    P   F  +G+ G  LG+  F+       +T++KTVT
Sbjct:   18 IQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIP-IDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASR-IFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGY----FVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGN-GRELGEYGFHE------YTEVKTVT 493          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Match: ALDH1A3 (aldehyde dehydrogenase 1 family member A3 [Source:HGNC Symbol;Acc:HGNC:409])

HSP 1 Score: 230.72 bits (587), Expect = 4.415e-68
Identity = 164/500 (32.80%), Postives = 256/500 (51.20%), Query Frame = 3
Query:   93 ALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES---WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVN-FICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYED-GNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            AL R   +  +  TK+FIN ++ +S++ +     NP+T + I  V +  KP++++AVE+   AF+    W +   L+R + + +L +L++RD   +AA  T + GK  L A    + G ++ + +     +   G+T+P    D ++V +   EP+GV   ITP+NFP ++ +W    +L CGNTM+LKP+E+ P   + L  L  EAGFPPG VN++ G    V   I  HP+I  I+F G  + G+ + E  S+ N KRV   +G KN  ++  DA  +  +       F   GQ C A S  VFV E     ++   +  AK   V +      + GP I +   ++I  LIESG KEGAK+   G     S  ED G F+ PT+ + V  +M   KEEIFGPV   L+  ++++ IK  N+  YG   A+FT +   A K    +  G V +N    +    P   F  S     G+    G+  +  +T++KTVT
Sbjct:   19 ALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIP---TDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASR-VFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGG-----SAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMS-----GNGRELGEYALAEYTEVKTVT 504          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Match: alh-8 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P52713])

HSP 1 Score: 706.827 bits (1823), Expect = 0.000e+0
Identity = 324/495 (65.45%), Postives = 403/495 (81.41%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAE 1580
             S +  + A P  K++I+G  V+S+TT++++L NPATN+VI+ VP  T+ EM+ AV+S   AF +W   S L RQQ MFKLQ LIKRD+KK+A +IT EQGKTL DAEGDV RGLQVVEH CS+ +L +GET+P +++D+D  SY+ PLGVTAGI PFNFPAMIPLWMFP++L  GNTM++KPSE+DPGA  +L+EL  EAG P G VN+IHGQH AVNFICD+P I+AISFVGGD AG+HIYERG+KNGKRVQ+N+GAKNHGV+M DA KE T+NQL  AAFGAAGQRCMAL+TAV VGE R W+P+++ +AKNLKV+ G +P  D+GP+ISK +K R+  LIES  KEGA++ LDG N+ V G+E+GNFVGPTIL  V P+M CY+EEIFGPVL+ +E + L++AI++INNNPYGNGTAIFT++GATARKFT ++ VGQ+G+NVPIPVPLPMFSFTGSRGSFLGD NFYGK G+ F+TQ KTVTQ W E   E
Sbjct:   18 FSTSKSAAAAPTVKLWIDGQAVESKTTDFVELTNPATNEVIAMVPNATQAEMQAAVDSAKNAFNTWKNTSPLTRQQCMFKLQALIKRDMKKLAESITIEQGKTLPDAEGDVSRGLQVVEHACSVPSLMMGETLPNVSRDMDTHSYRIPLGVTAGICPFNFPAMIPLWMFPVALATGNTMVIKPSEQDPGAAQLLVELAKEAGVPDGCVNIIHGQHSAVNFICDNPDIKAISFVGGDAAGKHIYERGAKNGKRVQSNMGAKNHGVIMADANKEQTLNQLTAAAFGAAGQRCMALTTAVLVGEARAWLPELVEKAKNLKVNAGWKPDTDIGPLISKQSKARVLRLIESAKKEGAQVPLDGSNITVPGFENGNFVGPTILAGVKPNMTCYREEIFGPVLVVMEAENLNEAIEIINNNPYGNGTAIFTSNGATARKFTNEVDVGQIGINVPIPVPLPMFSFTGSRGSFLGDLNFYGKAGIQFYTQWKTVTQYWNESLTE 512          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Match: alh-8 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P52713])

HSP 1 Score: 706.827 bits (1823), Expect = 0.000e+0
Identity = 324/495 (65.45%), Postives = 403/495 (81.41%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAE 1580
             S +  + A P  K++I+G  V+S+TT++++L NPATN+VI+ VP  T+ EM+ AV+S   AF +W   S L RQQ MFKLQ LIKRD+KK+A +IT EQGKTL DAEGDV RGLQVVEH CS+ +L +GET+P +++D+D  SY+ PLGVTAGI PFNFPAMIPLWMFP++L  GNTM++KPSE+DPGA  +L+EL  EAG P G VN+IHGQH AVNFICD+P I+AISFVGGD AG+HIYERG+KNGKRVQ+N+GAKNHGV+M DA KE T+NQL  AAFGAAGQRCMAL+TAV VGE R W+P+++ +AKNLKV+ G +P  D+GP+ISK +K R+  LIES  KEGA++ LDG N+ V G+E+GNFVGPTIL  V P+M CY+EEIFGPVL+ +E + L++AI++INNNPYGNGTAIFT++GATARKFT ++ VGQ+G+NVPIPVPLPMFSFTGSRGSFLGD NFYGK G+ F+TQ KTVTQ W E   E
Sbjct:   18 FSTSKSAAAAPTVKLWIDGQAVESKTTDFVELTNPATNEVIAMVPNATQAEMQAAVDSAKNAFNTWKNTSPLTRQQCMFKLQALIKRDMKKLAESITIEQGKTLPDAEGDVSRGLQVVEHACSVPSLMMGETLPNVSRDMDTHSYRIPLGVTAGICPFNFPAMIPLWMFPVALATGNTMVIKPSEQDPGAAQLLVELAKEAGVPDGCVNIIHGQHSAVNFICDNPDIKAISFVGGDAAGKHIYERGAKNGKRVQSNMGAKNHGVIMADANKEQTLNQLTAAAFGAAGQRCMALTTAVLVGEARAWLPELVEKAKNLKVNAGWKPDTDIGPLISKQSKARVLRLIESAKKEGAQVPLDGSNITVPGFENGNFVGPTILAGVKPNMTCYREEIFGPVLVVMEAENLNEAIEIINNNPYGNGTAIFTSNGATARKFTNEVDVGQIGINVPIPVPLPMFSFTGSRGSFLGDLNFYGKAGIQFYTQWKTVTQYWNESLTE 512          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Match: alh-8 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P52713])

HSP 1 Score: 706.827 bits (1823), Expect = 0.000e+0
Identity = 324/495 (65.45%), Postives = 403/495 (81.41%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAE 1580
             S +  + A P  K++I+G  V+S+TT++++L NPATN+VI+ VP  T+ EM+ AV+S   AF +W   S L RQQ MFKLQ LIKRD+KK+A +IT EQGKTL DAEGDV RGLQVVEH CS+ +L +GET+P +++D+D  SY+ PLGVTAGI PFNFPAMIPLWMFP++L  GNTM++KPSE+DPGA  +L+EL  EAG P G VN+IHGQH AVNFICD+P I+AISFVGGD AG+HIYERG+KNGKRVQ+N+GAKNHGV+M DA KE T+NQL  AAFGAAGQRCMAL+TAV VGE R W+P+++ +AKNLKV+ G +P  D+GP+ISK +K R+  LIES  KEGA++ LDG N+ V G+E+GNFVGPTIL  V P+M CY+EEIFGPVL+ +E + L++AI++INNNPYGNGTAIFT++GATARKFT ++ VGQ+G+NVPIPVPLPMFSFTGSRGSFLGD NFYGK G+ F+TQ KTVTQ W E   E
Sbjct:   18 FSTSKSAAAAPTVKLWIDGQAVESKTTDFVELTNPATNEVIAMVPNATQAEMQAAVDSAKNAFNTWKNTSPLTRQQCMFKLQALIKRDMKKLAESITIEQGKTLPDAEGDVSRGLQVVEHACSVPSLMMGETLPNVSRDMDTHSYRIPLGVTAGICPFNFPAMIPLWMFPVALATGNTMVIKPSEQDPGAAQLLVELAKEAGVPDGCVNIIHGQHSAVNFICDNPDIKAISFVGGDAAGKHIYERGAKNGKRVQSNMGAKNHGVIMADANKEQTLNQLTAAAFGAAGQRCMALTTAVLVGEARAWLPELVEKAKNLKVNAGWKPDTDIGPLISKQSKARVLRLIESAKKEGAQVPLDGSNITVPGFENGNFVGPTILAGVKPNMTCYREEIFGPVLVVMEAENLNEAIEIINNNPYGNGTAIFTSNGATARKFTNEVDVGQIGINVPIPVPLPMFSFTGSRGSFLGDLNFYGKAGIQFYTQWKTVTQYWNESLTE 512          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Match: alh-2 (ALdehyde deHydrogenase [Source:UniProtKB/TrEMBL;Acc:Q9TXM0])

HSP 1 Score: 214.927 bits (546), Expect = 1.545e-62
Identity = 148/484 (30.58%), Postives = 246/484 (50.83%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQ-HDAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLK----VSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            T +FIN +FVD+++ +  +  NPA  K++++V +  + +++ AVE+  +AF+    W +    +R   + +L +L++RD   +A+  + + GK   +A   D+   ++   +     +   G+T+P +  D    +  EP+GV   I P+NFP ++  W    +L  GNT+++K + + P + + +  L  EA FP G VN+I G+  DA   I  H  +  ++F G  + G+ I +  ++ N K+V   +G K+  ++  DA  E  + Q   A F   GQ C A S     G+  +   + +A+AK L     + +        GP I +   E I   IESG KEGA++V  G    V   + G FV PTI  NVN  M+  +EEIFGPV+I +  D++++ I+  NN  YG    + TN    A +    I  G V VN    V  P   F G + S +G     G+ G+  +T++KTVT
Sbjct:   34 TSLFINNEFVDAKSGKTFEFVNPANGKLLAKVAEGNRDDVDIAVEAAKKAFKIGSEWRRMDASHRGVLLNRLADLMERDRVILASLESLDNGKPYKEAYNIDLPISIKTFRYYAGYADKNHGKTIP-VGGDYFTYTRHEPVGVCGQIIPWNFPLLMQAWKLAPALAMGNTVVMKVAVKTPLSALHVASLIKEAQFPEGVVNIIPGRGTDAGEAIASHMDVDKVAFTGSTEVGKTIMKAAAESNVKKVTLELGGKSPNIVFADADLEEAVRQSHHALFFNQGQCCSAGSRTFVEGKIYD---EFVAKAKELVEKTVIGDPFDENTTQGPQIDESQVETIMKYIESGKKEGAQLVTGG----VKHGDQGYFVKPTIFANVNDQMKIAQEEIFGPVMIVIRFDSMEELIEKANNTIYGLAAGVVTNDLNKALQVANTIRAGSVWVNC-YDVFDPAAPFGGFKQSGIGRE--LGEYGLAAYTEVKTVT 506          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Match: alh-1 (ALdehyde deHydrogenase [Source:UniProtKB/TrEMBL;Acc:Q20780])

HSP 1 Score: 196.438 bits (498), Expect = 1.025e-55
Identity = 150/511 (29.35%), Postives = 244/511 (47.75%), Query Frame = 3
Query:   72 RLTLRVHALSRNY------LSTAIPK-TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDA-EGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQ-HDAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADL----GPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            R  LR    +RN       LS   P+ T +FIN +FV +++ +  +  NPA  KV+++V +  K ++  AV++   AF     W +     R   + +L +L++RD   +A+  + + GK    A   D+   ++ + +     +   G+T+P I  D    +  EP+GV   I P+NFP ++  W    +L  GNT+++K +E+ P + + +  L  EAGFP G VN+I G  H A   I  H  +  ++F G  + GR + +  ++ N K+V   +G K+  ++  DA    +++Q     F   GQ C A S     G+  +   D +AR+K L     +    DL    GP +     E I   I +G K+GA++V  G        + G+FV PTI  NV   M   +EEIFGPV+  +  DT+++ ++  NN  YG    + T     A         G V VN    V      F G + S +G     G+ G+  +T++KTVT
Sbjct:    3 RSALRATVQARNASGVPPGLSNMKPQYTGIFINNEFVPAKSGKTFETINPANGKVLAQVAEGDKTDVNIAVKAAQNAFRIGSEWRRMDASQRGVLLNRLADLMERDRVILASLESLDNGKPYAVAYNADLPLSIKTLRYYAGWADKNHGKTIP-IEGDYFTYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALAMGNTVVMKVAEQTPLSALHVAALTKEAGFPDGVVNIIPGYGHTAGQAISSHMDVDKVAFTGSTEVGRLVMKAAAESNVKKVTLELGGKSPNIIFADADLNDSVHQANHGLFFNQGQCCCAGSRTFVEGKIYD---DFVARSKELAEKAVIGDPFDLKTTQGPQVDGKQVETILKYIAAGKKDGAQLVTGGAKHG----DQGHFVKPTIFANVKDQMTIAQEEIFGPVMTIIRFDTMEELVEKANNTIYGLAAGVMTKDIDKALHIANATRAGSVWVNC-YDVFDAAAPFGGFKQSGIGRE--LGEYGLEAYTEVKTVT 502          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Match: CG17896 (gene:FBgn0023537 transcript:FBtr0070092)

HSP 1 Score: 727.243 bits (1876), Expect = 0.000e+0
Identity = 344/510 (67.45%), Postives = 421/510 (82.55%), Query Frame = 3
Query:   90 HALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            H   R+Y S+A P TK+FI+G FV+S+T EWID+ +PATN+V++RVPK T+ EM+ A+ES  +AF SWS +S+L RQQ MFKLQ LIK ++ ++A NIT EQGKTL DAEGDV+RGLQVVEHCCSI +LQ+GET+  +A+D+D  S   PLGVTAG+ PFNFPAMIPLWMFP+++  GNTM+LKPSER PGATM+LMEL  EAG PPG VNVIHGQHDAVNFICD P+I+A+SFVG D+AG++IYER  KNGKRVQ+N+GAKNHG+++ DA KE+T+NQL GAAFGAAGQRCMALSTAVFVG+ + WIPD++ RA+ LKV+ G  PG D+GPVIS  +++RI+ LIESG KEGAK++LDGR + V GYEDG FVGPTIL++V PSM+CY EEIFGPVL+ L+ DTLDDAI ++N NPYGNGTA+FT +GA ARKF  +I  GQVGVNVPIPVPLPMFSFTG+RGSF GD +FYGKQG+ F+TQ KTVTQLWR+ D  H    +A   PTM
Sbjct:   13 HLAKRSY-SSAAPTTKLFIDGKFVESKTNEWIDVHDPATNQVVTRVPKATQAEMQAALESNKKAFRSWSNQSILTRQQVMFKLQALIKENMGELAKNITKEQGKTLADAEGDVLRGLQVVEHCCSIPSLQMGETVANVARDMDTYSLVLPLGVTAGVAPFNFPAMIPLWMFPVAITTGNTMLLKPSERVPGATMLLMELLNEAGCPPGVVNVIHGQHDAVNFICDAPEIKAVSFVGSDQAGKYIYERAGKNGKRVQSNMGAKNHGIILGDANKENTLNQLAGAAFGAAGQRCMALSTAVFVGDAQAWIPDLVERAQKLKVNAGHVPGTDVGPVISAASRQRINDLIESGVKEGAKLILDGRKITVPGYEDGYFVGPTILSDVTPSMKCYTEEIFGPVLVILKADTLDDAIGIVNANPYGNGTAVFTTNGAAARKFVNEIDAGQVGVNVPIPVPLPMFSFTGTRGSFRGDHHFYGKQGIKFYTQTKTVTQLWRKTDVTHTQ--AAVAMPTM 519          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Match: CG17896 (gene:FBgn0023537 transcript:FBtr0070093)

HSP 1 Score: 727.243 bits (1876), Expect = 0.000e+0
Identity = 344/510 (67.45%), Postives = 421/510 (82.55%), Query Frame = 3
Query:   90 HALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            H   R+Y S+A P TK+FI+G FV+S+T EWID+ +PATN+V++RVPK T+ EM+ A+ES  +AF SWS +S+L RQQ MFKLQ LIK ++ ++A NIT EQGKTL DAEGDV+RGLQVVEHCCSI +LQ+GET+  +A+D+D  S   PLGVTAG+ PFNFPAMIPLWMFP+++  GNTM+LKPSER PGATM+LMEL  EAG PPG VNVIHGQHDAVNFICD P+I+A+SFVG D+AG++IYER  KNGKRVQ+N+GAKNHG+++ DA KE+T+NQL GAAFGAAGQRCMALSTAVFVG+ + WIPD++ RA+ LKV+ G  PG D+GPVIS  +++RI+ LIESG KEGAK++LDGR + V GYEDG FVGPTIL++V PSM+CY EEIFGPVL+ L+ DTLDDAI ++N NPYGNGTA+FT +GA ARKF  +I  GQVGVNVPIPVPLPMFSFTG+RGSF GD +FYGKQG+ F+TQ KTVTQLWR+ D  H    +A   PTM
Sbjct:    4 HLAKRSY-SSAAPTTKLFIDGKFVESKTNEWIDVHDPATNQVVTRVPKATQAEMQAALESNKKAFRSWSNQSILTRQQVMFKLQALIKENMGELAKNITKEQGKTLADAEGDVLRGLQVVEHCCSIPSLQMGETVANVARDMDTYSLVLPLGVTAGVAPFNFPAMIPLWMFPVAITTGNTMLLKPSERVPGATMLLMELLNEAGCPPGVVNVIHGQHDAVNFICDAPEIKAVSFVGSDQAGKYIYERAGKNGKRVQSNMGAKNHGIILGDANKENTLNQLAGAAFGAAGQRCMALSTAVFVGDAQAWIPDLVERAQKLKVNAGHVPGTDVGPVISAASRQRINDLIESGVKEGAKLILDGRKITVPGYEDGYFVGPTILSDVTPSMKCYTEEIFGPVLVILKADTLDDAIGIVNANPYGNGTAVFTTNGAAARKFVNEIDAGQVGVNVPIPVPLPMFSFTGTRGSFRGDHHFYGKQGIKFYTQTKTVTQLWRKTDVTHTQ--AAVAMPTM 510          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Match: CG31075 (gene:FBgn0051075 transcript:FBtr0085080)

HSP 1 Score: 218.009 bits (554), Expect = 8.754e-64
Identity = 160/485 (32.99%), Postives = 243/485 (50.10%), Query Frame = 3
Query:  120 AIPK-TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALS-TAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            A PK TK+FIN +FVDS + +     NPAT+K I +V +  K +++ AV++  +AF     W + S L R   M KL  L+ RD   +A+  T + GK   +A  DV   +  +++    T+   G+T+P  A     ++ KEP+GV   I P+N+P ++  W +  +L  G T+I+KP+E+ P   + +  L  EAGFP G +NV++G    A   I  HP I  ++F G  + GR + +   + N KRV   +G K+  V+  DA  +  +     A F   GQ C A S T V       ++    A+AK  KV    +     GP I      ++   IESG KEGAK+   G+ +   G+    FV PT+ ++V   M   +EEIFGPV    +  +L++ I   NN  YG    + TN    A KF   +  G V +N    V LP   F G + S +G     GK G++ + + KT+T
Sbjct:    6 AKPKYTKLFINNEFVDSVSGKTFATFNPATSKEIVQVSEGDKADIDLAVKAAKKAFHRDSEWRKLSPLQRTNLMNKLCALMDRDKAFLASLETQDNGKPYAEALFDVTYSILTLQYYAGWTDKFFGDTIP--AGGFVSMTRKEPVGVVGQIIPWNYPLLMLAWKWGPALAVGCTIIMKPAEQTPLTALHMAALAKEAGFPAGVINVVNGFGPTAGAAISAHPDIAKVAFTGSVEIGRIVMQAAATSNLKRVSLELGGKSPVVVFDDADIDFAVETTHEALFSNHGQSCCAGSRTYVHEKIYDEFVAKAAAKAKARKVGNPFEQNVQQGPQIDDDMLTKVLGYIESGKKEGAKLQAGGKRIGNVGF----FVEPTVFSDVKDDMRIAQEEIFGPVQSIFKFSSLEEMIDRANNVQYGLAAGVITNDINKALKFANNVDAGSVWINCYDAV-LPSTPFGGYKHSGIGRE--LGKDGLDNYLETKTIT 481          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Match: CG31075 (gene:FBgn0051075 transcript:FBtr0307506)

HSP 1 Score: 214.157 bits (544), Expect = 4.050e-62
Identity = 155/479 (32.36%), Postives = 239/479 (49.90%), Query Frame = 3
Query:  135 KMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALS-TAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            ++FIN +FVDS + +     NPAT+K I +V +  K +++ AV++  +AF     W + S L R   M KL  L+ RD   +A+  T + GK   +A  DV   +  +++    T+   G+T+P  A     ++ KEP+GV   I P+N+P ++  W +  +L  G T+I+KP+E+ P   + +  L  EAGFP G +NV++G    A   I  HP I  ++F G  + GR + +   + N KRV   +G K+  V+  DA  +  +     A F   GQ C A S T V       ++    A+AK  KV    +     GP I      ++   IESG KEGAK+   G+ +   G+    FV PT+ ++V   M   +EEIFGPV    +  +L++ I   NN  YG    + TN    A KF   +  G V +N    V LP   F G + S +G     GK G++ + + KT+T
Sbjct:   35 QLFINNEFVDSVSGKTFATFNPATSKEIVQVSEGDKADIDLAVKAAKKAFHRDSEWRKLSPLQRTNLMNKLCALMDRDKAFLASLETQDNGKPYAEALFDVTYSILTLQYYAGWTDKFFGDTIP--AGGFVSMTRKEPVGVVGQIIPWNYPLLMLAWKWGPALAVGCTIIMKPAEQTPLTALHMAALAKEAGFPAGVINVVNGFGPTAGAAISAHPDIAKVAFTGSVEIGRIVMQAAATSNLKRVSLELGGKSPVVVFDDADIDFAVETTHEALFSNHGQSCCAGSRTYVHEKIYDEFVAKAAAKAKARKVGNPFEQNVQQGPQIDDDMLTKVLGYIESGKKEGAKLQAGGKRIGNVGF----FVEPTVFSDVKDDMRIAQEEIFGPVQSIFKFSSLEEMIDRANNVQYGLAAGVITNDINKALKFANNVDAGSVWINCYDAV-LPSTPFGGYKHSGIGRE--LGKDGLDNYLETKTIT 504          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Match: Ssadh (gene:FBgn0039349 transcript:FBtr0100569)

HSP 1 Score: 207.994 bits (528), Expect = 8.022e-60
Identity = 134/493 (27.18%), Postives = 248/493 (50.30%), Query Frame = 3
Query:   93 ALSRNYLSTAIPKTKMFINGDFVDSQTTEW-IDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES--WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV--NFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
            +L R    +A+ + K  ++G +VDS   +   ++ NPA   VI +VP  T  + ++A+++  +A+ES  W   +  +R   + K  +LI++  ++IA  +T+E GK + +++G+V  G   VE          GE +P  + + +I+  K+P+GV A ITP+NFP  +       +L  G T+++KPSE  P   + + +L V+AG P G +NV+     A   +  C  P +R ISF G  + G+ ++   +   KR+   +G     ++   A  E  ++  + + F   GQ C++ +   FV ++    ++  +  R + LK+ +G      +GP+I++    ++   +E    + A I+L G+ L   G     F  PTI+T+V PS + Y EE+FGPV+  +     ++A+K  N+   G     ++ +     +  +++ VG VGVN  I +      F G + S +G      K G++ +  +K +
Sbjct:   16 SLCRMRGFSALVQDKALVDGAWVDSSNAKATFEVRNPANGAVIGKVPNMTVADAQKAIDAAKQAYESKEWRSLTAKDRSNLLKKWHKLIEQHSQEIAEIMTAESGKPINESKGEVAYGNAFVEWFAEEARRIYGEIVPSASPNREIIVMKQPIGVAALITPWNFPMAMITRKAGAALAAGCTVVVKPSEDTPLTALAVAKLAVDAGIPKGVINVVTTNKAAPIGDLFCKSPDVRGISFTGSTEVGKLLFRNSADGIKRICLELGGNAPFIVFDSADIEKAVDGAMASKFRNCGQTCVS-ANRFFVQDSVYDKFVGQLKKRVEALKIGDGQGCDVQIGPLINEMQFNKVSGFVEDARSKKANIILGGQPLPDKG---SLFYAPTIVTDVPPSAQLYSEEVFGPVVSIIRFRDEEEAVKKANDTRRGLAGYFYSENLQQVFRVAKRLEVGMVGVNEGI-ISAAEAPFGGVKESGVGREG--SKHGIDDYVDIKYI 501          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Match: aldh6a1 (aldehyde dehydrogenase 6 family, member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-9192])

HSP 1 Score: 758.444 bits (1957), Expect = 0.000e+0
Identity = 346/520 (66.54%), Postives = 434/520 (83.46%), Query Frame = 3
Query:   60 IASKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            I  +R+ L+V    R   S+++P TK+FI+G FV+S++++W+D+ NPAT++VI RVPK T+ EM  AV+SC+ AF++WS+ S+L RQQ  F+ Q+LIK ++K++A  IT EQGKTL DAEGDV RGLQVVEH CSIT+L LGET+P + KD+D  +Y+ P+GV AGI PFNFPAMIPLWMFP+ +VCGNT +LKPSER PG TMML +L  +AG P GT+N+IHGQHDAVNFICDHP IRAISFVG ++AG +IYERGSKNGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTA+ VGE R+W+P+++ RAK+L+V+ G QPGAD+GP+IS  AKER++SLI+SG  EGAK++LDGRN+KV GYE+GNFVGPTI++NV P M CYKEEIFGPVL+ LE D+LD+AIK++N NPYGNGTAIFT +GA ARK++ ++ VGQ+GVNVPIPVPLPMFSFTGSRGSF GDTNFYGKQG+ F+TQ+KT+T  W+ EDA    K  A   PTM
Sbjct:    9 IVKRRVPLQV---GRMCYSSSVPTTKLFIDGKFVESKSSQWLDIHNPATSEVIGRVPKATQEEMLAAVDSCSRAFQTWSETSILARQQIFFRYQQLIKDNIKELAKLITLEQGKTLADAEGDVFRGLQVVEHTCSITSLMLGETLPSLTKDMDTYTYRLPIGVCAGIAPFNFPAMIPLWMFPMGMVCGNTYLLKPSERVPGCTMMLAKLLQDAGAPDGTLNIIHGQHDAVNFICDHPAIRAISFVGSNQAGEYIYERGSKNGKRVQSNMGAKNHGVIMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEARDWLPELVERAKSLRVNAGDQPGADVGPLISPQAKERVNSLIQSGVDEGAKVLLDGRNVKVKGYENGNFVGPTIISNVTPEMTCYKEEIFGPVLVVLEADSLDEAIKIVNKNPYGNGTAIFTTNGAAARKYSHEVDVGQIGVNVPIPVPLPMFSFTGSRGSFRGDTNFYGKQGIQFYTQIKTITSQWKAEDATL--KSPAVTMPTM 523          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Match: aldh9a1a.1 (aldehyde dehydrogenase 9 family, member A1a, tandem duplicate 1 [Source:NCBI gene;Acc:100005587])

HSP 1 Score: 226.098 bits (575), Expect = 1.670e-66
Identity = 140/462 (30.30%), Postives = 228/462 (49.35%), Query Frame = 3
Query:  195 NPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSG--YEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV----PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             PAT +V+  +  C + E+++A++S   A+  WSQ S + R + M +   +I+     IA    +  GK++ +AE D+    Q +E+   I     G+   +PG +      + +EPLGV  GI  +N+P  I  W    +L CGN M+ KPS   P   +ML E+  EAG P G  NV+ G  +  + +C HP +  +SF G    G+ + E  +K+ K+V   +G K+  ++  D   E+ I   + A F   G+ C    T VFV       ++ +++ R K + V + +  G  +G +ISK   E++   I+   ++G K++  G     +    +DG FV P +L N    M C KEEIFGPV+  L  DT ++ ++  NN  +G  + +FT   A A +    +  G   +N     P+ VP     F G + S  G  N  G   + +++QLKTV
Sbjct:   47 EPATGRVLCDMIPCGEEEVDQAIKSAHSAYLKWSQLSGMERSRIMLEAARIIRERRNAIAKAEVANNGKSITEAEVDIDVAWQCIEYYAGIAPTLSGQHIQLPGGSFAY---TRREPLGVCVGIGAWNYPFQIAAWKSAPALACGNAMVFKPSPMTPVTAVMLAEIYKEAGVPDGLFNVVQGGAETGSLLCHHPMVAKVSFTGSVPTGKKVMEMAAKSVKQVTLELGGKSPLIIFKDCELENAIKGALMANFLTQGEVCCN-GTRVFVQREIMPKFLEEVVKRTKAISVGDPLCEGTRMGALISKPHMEKVLGFIKQAKEQGGKVLCGGERFVPNDPKLKDGYFVSPCVLDNCRDDMTCVKEEIFGPVMSVLPFDTEEEVLQRANNTTFGLASGVFTRDIARAHRVAANLQAGTCYINNYNVGPVEVP-----FGGYKMSGFGREN--GTVTIEYYSQLKTV 497          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Match: aldh9a1a.1 (aldehyde dehydrogenase 9 family, member A1a, tandem duplicate 1 [Source:NCBI gene;Acc:100005587])

HSP 1 Score: 226.098 bits (575), Expect = 1.670e-66
Identity = 140/462 (30.30%), Postives = 228/462 (49.35%), Query Frame = 3
Query:  195 NPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSG--YEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV----PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             PAT +V+  +  C + E+++A++S   A+  WSQ S + R + M +   +I+     IA    +  GK++ +AE D+    Q +E+   I     G+   +PG +      + +EPLGV  GI  +N+P  I  W    +L CGN M+ KPS   P   +ML E+  EAG P G  NV+ G  +  + +C HP +  +SF G    G+ + E  +K+ K+V   +G K+  ++  D   E+ I   + A F   G+ C    T VFV       ++ +++ R K + V + +  G  +G +ISK   E++   I+   ++G K++  G     +    +DG FV P +L N    M C KEEIFGPV+  L  DT ++ ++  NN  +G  + +FT   A A +    +  G   +N     P+ VP     F G + S  G  N  G   + +++QLKTV
Sbjct:   47 EPATGRVLCDMIPCGEEEVDQAIKSAHSAYLKWSQLSGMERSRIMLEAARIIRERRNAIAKAEVANNGKSITEAEVDIDVAWQCIEYYAGIAPTLSGQHIQLPGGSFAY---TRREPLGVCVGIGAWNYPFQIAAWKSAPALACGNAMVFKPSPMTPVTAVMLAEIYKEAGVPDGLFNVVQGGAETGSLLCHHPMVAKVSFTGSVPTGKKVMEMAAKSVKQVTLELGGKSPLIIFKDCELENAIKGALMANFLTQGEVCCN-GTRVFVQREIMPKFLEEVVKRTKAISVGDPLCEGTRMGALISKPHMEKVLGFIKQAKEQGGKVLCGGERFVPNDPKLKDGYFVSPCVLDNCRDDMTCVKEEIFGPVMSVLPFDTEEEVLQRANNTTFGLASGVFTRDIARAHRVAANLQAGTCYINNYNVGPVEVP-----FGGYKMSGFGREN--GTVTIEYYSQLKTV 497          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Match: aldh9a1a.1 (aldehyde dehydrogenase 9 family, member A1a, tandem duplicate 1 [Source:NCBI gene;Acc:100005587])

HSP 1 Score: 226.098 bits (575), Expect = 1.670e-66
Identity = 140/462 (30.30%), Postives = 228/462 (49.35%), Query Frame = 3
Query:  195 NPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSG--YEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV----PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             PAT +V+  +  C + E+++A++S   A+  WSQ S + R + M +   +I+     IA    +  GK++ +AE D+    Q +E+   I     G+   +PG +      + +EPLGV  GI  +N+P  I  W    +L CGN M+ KPS   P   +ML E+  EAG P G  NV+ G  +  + +C HP +  +SF G    G+ + E  +K+ K+V   +G K+  ++  D   E+ I   + A F   G+ C    T VFV       ++ +++ R K + V + +  G  +G +ISK   E++   I+   ++G K++  G     +    +DG FV P +L N    M C KEEIFGPV+  L  DT ++ ++  NN  +G  + +FT   A A +    +  G   +N     P+ VP     F G + S  G  N  G   + +++QLKTV
Sbjct:   47 EPATGRVLCDMIPCGEEEVDQAIKSAHSAYLKWSQLSGMERSRIMLEAARIIRERRNAIAKAEVANNGKSITEAEVDIDVAWQCIEYYAGIAPTLSGQHIQLPGGSFAY---TRREPLGVCVGIGAWNYPFQIAAWKSAPALACGNAMVFKPSPMTPVTAVMLAEIYKEAGVPDGLFNVVQGGAETGSLLCHHPMVAKVSFTGSVPTGKKVMEMAAKSVKQVTLELGGKSPLIIFKDCELENAIKGALMANFLTQGEVCCN-GTRVFVQREIMPKFLEEVVKRTKAISVGDPLCEGTRMGALISKPHMEKVLGFIKQAKEQGGKVLCGGERFVPNDPKLKDGYFVSPCVLDNCRDDMTCVKEEIFGPVMSVLPFDTEEEVLQRANNTTFGLASGVFTRDIARAHRVAANLQAGTCYINNYNVGPVEVP-----FGGYKMSGFGREN--GTVTIEYYSQLKTV 497          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Match: aldh9a1a.1 (aldehyde dehydrogenase 9 family, member A1a, tandem duplicate 1 [Source:NCBI gene;Acc:100005587])

HSP 1 Score: 226.098 bits (575), Expect = 1.670e-66
Identity = 140/462 (30.30%), Postives = 228/462 (49.35%), Query Frame = 3
Query:  195 NPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSG--YEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV----PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             PAT +V+  +  C + E+++A++S   A+  WSQ S + R + M +   +I+     IA    +  GK++ +AE D+    Q +E+   I     G+   +PG +      + +EPLGV  GI  +N+P  I  W    +L CGN M+ KPS   P   +ML E+  EAG P G  NV+ G  +  + +C HP +  +SF G    G+ + E  +K+ K+V   +G K+  ++  D   E+ I   + A F   G+ C    T VFV       ++ +++ R K + V + +  G  +G +ISK   E++   I+   ++G K++  G     +    +DG FV P +L N    M C KEEIFGPV+  L  DT ++ ++  NN  +G  + +FT   A A +    +  G   +N     P+ VP     F G + S  G  N  G   + +++QLKTV
Sbjct:   47 EPATGRVLCDMIPCGEEEVDQAIKSAHSAYLKWSQLSGMERSRIMLEAARIIRERRNAIAKAEVANNGKSITEAEVDIDVAWQCIEYYAGIAPTLSGQHIQLPGGSFAY---TRREPLGVCVGIGAWNYPFQIAAWKSAPALACGNAMVFKPSPMTPVTAVMLAEIYKEAGVPDGLFNVVQGGAETGSLLCHHPMVAKVSFTGSVPTGKKVMEMAAKSVKQVTLELGGKSPLIIFKDCELENAIKGALMANFLTQGEVCCN-GTRVFVQREIMPKFLEEVVKRTKAISVGDPLCEGTRMGALISKPHMEKVLGFIKQAKEQGGKVLCGGERFVPNDPKLKDGYFVSPCVLDNCRDDMTCVKEEIFGPVMSVLPFDTEEEVLQRANNTTFGLASGVFTRDIARAHRVAANLQAGTCYINNYNVGPVEVP-----FGGYKMSGFGREN--GTVTIEYYSQLKTV 497          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Match: tlr3 (toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-491049])

HSP 1 Score: 748.429 bits (1931), Expect = 0.000e+0
Identity = 343/502 (68.33%), Postives = 420/502 (83.67%), Query Frame = 3
Query:  114 STAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            S A+P+TK+FING FVDS+T+EWID+ NPATN+VI RVP+ T+ EME AV +C   F +WS+ S+LNRQQ   + Q+LIK +LK++A  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGET+  + KD+D  +++ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM L++L  +AG P GT+N+IHGQH AVNFICDHP IRAISFVG ++AG +IY+RGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ +AK L+V+ G QPGADLGP+IS  AK+R+ SL+ESG +EGA ++LDGR++ V G+E+GNFVGPTIL NV P M CYKEEIFGPVL+ LE DTLDDA+K++N+NPYGNGTAIFT +GATARK+T  + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQGV+F+TQLKTVT  W+EED+   S   A   PTM
Sbjct:   22 SAAVPRTKLFINGQFVDSETSEWIDVHNPATNEVIGRVPQATQSEMEAAVNACQRTFPAWSETSILNRQQIFLRYQQLIKDNLKEVARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETLSSLTKDMDTFTFRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTYLMKPSERVPGATMFLVQLLQDAGVPDGTINIIHGQHAAVNFICDHPAIRAISFVGSNQAGEYIYDRGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKQWLPELVEKAKKLQVNAGDQPGADLGPLISPAAKDRVCSLVESGVREGATLLLDGRDVNVKGFENGNFVGPTILANVQPHMTCYKEEIFGPVLVILEADTLDDALKIVNSNPYGNGTAIFTTNGATARKYTHLVDVGQVGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGVHFYTQLKTVTSQWKEEDSTLTSP--AVVMPTM 521          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Match: tlr3 (toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-491049])

HSP 1 Score: 745.347 bits (1923), Expect = 0.000e+0
Identity = 341/503 (67.79%), Postives = 419/503 (83.30%), Query Frame = 3
Query:  111 LSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            + +  P+TK+FING FVDS+T+EWID+ NPATN+VI RVP+ T+ EME AV +C   F +WS+ S+LNRQQ   + Q+LIK +LK++A  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGET+  + KD+D  +++ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM L++L  +AG P GT+N+IHGQH AVNFICDHP IRAISFVG ++AG +IY+RGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ +AK L+V+ G QPGADLGP+IS  AK+R+ SL+ESG +EGA ++LDGR++ V G+E+GNFVGPTIL NV P M CYKEEIFGPVL+ LE DTLDDA+K++N+NPYGNGTAIFT +GATARK+T  + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQGV+F+TQLKTVT  W+EED+   S   A   PTM
Sbjct:   46 IKSVFPRTKLFINGQFVDSETSEWIDVHNPATNEVIGRVPQATQSEMEAAVNACQRTFPAWSETSILNRQQIFLRYQQLIKDNLKEVARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETLSSLTKDMDTFTFRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTYLMKPSERVPGATMFLVQLLQDAGVPDGTINIIHGQHAAVNFICDHPAIRAISFVGSNQAGEYIYDRGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKQWLPELVEKAKKLQVNAGDQPGADLGPLISPAAKDRVCSLVESGVREGATLLLDGRDVNVKGFENGNFVGPTILANVQPHMTCYKEEIFGPVLVILEADTLDDALKIVNSNPYGNGTAIFTTNGATARKYTHLVDVGQVGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGVHFYTQLKTVTSQWKEEDSTLTSP--AVVMPTM 546          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Match: tlr3 (toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-491049])

HSP 1 Score: 743.036 bits (1917), Expect = 0.000e+0
Identity = 341/498 (68.47%), Postives = 417/498 (83.73%), Query Frame = 3
Query:  126 PKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            P+TK+FING FVDS+T+EWID+ NPATN+VI RVP+ T+ EME AV +C   F +WS+ S+LNRQQ   + Q+LIK +LK++A  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGET+  + KD+D  +++ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM L++L  +AG P GT+N+IHGQH AVNFICDHP IRAISFVG ++AG +IY+RGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ +AK L+V+ G QPGADLGP+IS  AK+R+ SL+ESG +EGA ++LDGR++ V G+E+GNFVGPTIL NV P M CYKEEIFGPVL+ LE DTLDDA+K++N+NPYGNGTAIFT +GATARK+T  + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQGV+F+TQLKTVT  W+EED+   S   A   PTM
Sbjct:   20 PRTKLFINGQFVDSETSEWIDVHNPATNEVIGRVPQATQSEMEAAVNACQRTFPAWSETSILNRQQIFLRYQQLIKDNLKEVARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETLSSLTKDMDTFTFRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTYLMKPSERVPGATMFLVQLLQDAGVPDGTINIIHGQHAAVNFICDHPAIRAISFVGSNQAGEYIYDRGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKQWLPELVEKAKKLQVNAGDQPGADLGPLISPAAKDRVCSLVESGVREGATLLLDGRDVNVKGFENGNFVGPTILANVQPHMTCYKEEIFGPVLVILEADTLDDALKIVNSNPYGNGTAIFTTNGATARKYTHLVDVGQVGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGVHFYTQLKTVTSQWKEEDSTLTSP--AVVMPTM 515          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Match: tlr3 (toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-491049])

HSP 1 Score: 742.651 bits (1916), Expect = 0.000e+0
Identity = 341/498 (68.47%), Postives = 417/498 (83.73%), Query Frame = 3
Query:  126 PKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            P+TK+FING FVDS+T+EWID+ NPATN+VI RVP+ T+ EME AV +C   F +WS+ S+LNRQQ   + Q+LIK +LK++A  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGET+  + KD+D  +++ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM L++L  +AG P GT+N+IHGQH AVNFICDHP IRAISFVG ++AG +IY+RGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ +AK L+V+ G QPGADLGP+IS  AK+R+ SL+ESG +EGA ++LDGR++ V G+E+GNFVGPTIL NV P M CYKEEIFGPVL+ LE DTLDDA+K++N+NPYGNGTAIFT +GATARK+T  + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQGV+F+TQLKTVT  W+EED+   S   A   PTM
Sbjct:   27 PRTKLFINGQFVDSETSEWIDVHNPATNEVIGRVPQATQSEMEAAVNACQRTFPAWSETSILNRQQIFLRYQQLIKDNLKEVARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETLSSLTKDMDTFTFRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTYLMKPSERVPGATMFLVQLLQDAGVPDGTINIIHGQHAAVNFICDHPAIRAISFVGSNQAGEYIYDRGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKQWLPELVEKAKKLQVNAGDQPGADLGPLISPAAKDRVCSLVESGVREGATLLLDGRDVNVKGFENGNFVGPTILANVQPHMTCYKEEIFGPVLVILEADTLDDALKIVNSNPYGNGTAIFTTNGATARKYTHLVDVGQVGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGVHFYTQLKTVTSQWKEEDSTLTSP--AVVMPTM 522          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Match: tlr3 (toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-491049])

HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0
Identity = 341/498 (68.47%), Postives = 417/498 (83.73%), Query Frame = 3
Query:  126 PKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            P+TK+FING FVDS+T+EWID+ NPATN+VI RVP+ T+ EME AV +C   F +WS+ S+LNRQQ   + Q+LIK +LK++A  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGET+  + KD+D  +++ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM L++L  +AG P GT+N+IHGQH AVNFICDHP IRAISFVG ++AG +IY+RGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ +AK L+V+ G QPGADLGP+IS  AK+R+ SL+ESG +EGA ++LDGR++ V G+E+GNFVGPTIL NV P M CYKEEIFGPVL+ LE DTLDDA+K++N+NPYGNGTAIFT +GATARK+T  + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQGV+F+TQLKTVT  W+EED+   S   A   PTM
Sbjct:   31 PRTKLFINGQFVDSETSEWIDVHNPATNEVIGRVPQATQSEMEAAVNACQRTFPAWSETSILNRQQIFLRYQQLIKDNLKEVARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETLSSLTKDMDTFTFRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTYLMKPSERVPGATMFLVQLLQDAGVPDGTINIIHGQHAAVNFICDHPAIRAISFVGSNQAGEYIYDRGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKQWLPELVEKAKKLQVNAGDQPGADLGPLISPAAKDRVCSLVESGVREGATLLLDGRDVNVKGFENGNFVGPTILANVQPHMTCYKEEIFGPVLVILEADTLDDALKIVNSNPYGNGTAIFTTNGATARKYTHLVDVGQVGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGVHFYTQLKTVTSQWKEEDSTLTS--PAVVMPTM 526          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Match: Aldh6a1 (aldehyde dehydrogenase family 6, subfamily A1 [Source:MGI Symbol;Acc:MGI:1915077])

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 344/499 (68.94%), Postives = 422/499 (84.57%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI+G FV+S++ +WID+ NPATN+V+ RVP+ TK EM+ AVESC  AF +W+  S+L+RQQ + + Q+LIK +LK+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQHDAVNFICDHP I+AISFVG ++AG +I+ERGS+NGKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTA+ VGE + W+P+++ RAKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV PSM CYKEEIFGPVL+ LE +TLD+AIK++N+NPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGRVPQSTKAEMDAAVESCKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHDAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVDRAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRRIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFGPVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGATARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 533          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Match: Aldh1a7 (aldehyde dehydrogenase family 1, subfamily A7 [Source:MGI Symbol;Acc:MGI:1347050])

HSP 1 Score: 231.491 bits (589), Expect = 1.355e-68
Identity = 162/489 (33.13%), Postives = 247/489 (50.51%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN--VPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN ++ DS +++   + NPAT +VI  V +  K ++++AV++  +AF+    W       R + + KL +L++RD   +A   +   GK    A   DV   ++ +++     +   G+T+P    D +I +Y  +EP+GV   I P+N P +I  W    +L CGNT+++KP+E+ P   + +  L  EAGFPPG VN++ G    A   I  H  I  +SF G  + G+ I E  G  N KRV   +G K+  ++  DA  +S +       F   GQ C+A S  +FV E+    ++   + RAK   +   +  G + GP I K    +I  LIESG KEGAK+   G      G+    FV PT+ +NV   M   KEEIFGPV   ++  ++DD IK  NN  YG    +FT     A   +  +  G V VN  + +PV  P   F  S     G+    G+ G+  +T+LKTV 
Sbjct:   18 IQHTKIFINNEWHDSVSSKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPWRTMDASERGRLLNKLADLMERDRLLLATMESMNAGKVFAHAYLLDVEISIKALQYFAGWADKIHGQTIP---SDGNIFTYTRREPIGVCGQIIPWNGPLIIFTWKLGPALSCGNTVVVKPAEQTPLTALHMASLIKEAGFPPGVVNIVPGYGPTAGGAISSHMDIDKVSFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDSAVEFAHQGVFFHQGQICVAASR-LFVEESIYDEFVRRSVERAKKYILGNPLNSGINQGPQIDKEQHNKILGLIESGKKEGAKLECGGGRWGNKGF----FVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSMDDVIKRANNTTYGLAAGVFTKDLDKAITVSSALQAGMVWVNCYLAVPVQCPFGGFKMS-----GNGRELGEHGLYEYTELKTVA 493          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Match: Aldh1a1 (aldehyde dehydrogenase family 1, subfamily A1 [Source:MGI Symbol;Acc:MGI:1353450])

HSP 1 Score: 231.491 bits (589), Expect = 1.473e-68
Identity = 160/489 (32.72%), Postives = 248/489 (50.72%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAE-GDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSF--LGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN ++ +S + +   + NPAT +VI  V +  K ++++AV++  +AF+    W       R + + KL +L++RD   +A       GK   +A   D+   ++ +++C    +   G+T+P    D DI +Y  +EP+GV   I P+NFP ++ +W    +L CGNT+++KP+E+ P   + L  L  EAGFPPG VN++ G    A   I  H  +  ++F G  + G+ I E  G  N KRV   +G K+  ++  DA  +  +       F   GQ C+A S  +FV E+    ++   + RAK   +   + PG + GP I K   ++I  LIESG KEGAK+   G      G+    FV PT+ +NV   M   KEEIFGPV   ++  ++DD IK  NN  YG    +FT     A   +  +  G V VN  +     M S     G F   G+    G+ G+  +T+LKTV 
Sbjct:   18 IQHTKIFINNEWHNSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPWRTMDASERGRLLNKLADLMERDRLLLATMEALNGGKVFANAYLSDLGGCIKALKYCAGWADKIHGQTIP---SDGDIFTYTRREPIGVCGQIIPWNFPMLMFIWKIGPALSCGNTVVVKPAEQTPLTALHLASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDIAVEFAHHGVFYHQGQCCVAASR-IFVEESVYDEFVKRSVERAKKYVLGNPLTPGINQGPQIDKEQHDKILDLIESGKKEGAKLECGGGRWGNKGF----FVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSVDDVIKRANNTTYGLAAGLFTKDLDKAITVSSALQAGVVWVNCYM-----MLSAQCPFGGFKMSGNGRELGEHGLYEYTELKTVA 493          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Match: Aldh1a1 (aldehyde dehydrogenase family 1, subfamily A1 [Source:MGI Symbol;Acc:MGI:1353450])

HSP 1 Score: 231.491 bits (589), Expect = 1.473e-68
Identity = 160/489 (32.72%), Postives = 248/489 (50.72%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAE-GDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSF--LGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN ++ +S + +   + NPAT +VI  V +  K ++++AV++  +AF+    W       R + + KL +L++RD   +A       GK   +A   D+   ++ +++C    +   G+T+P    D DI +Y  +EP+GV   I P+NFP ++ +W    +L CGNT+++KP+E+ P   + L  L  EAGFPPG VN++ G    A   I  H  +  ++F G  + G+ I E  G  N KRV   +G K+  ++  DA  +  +       F   GQ C+A S  +FV E+    ++   + RAK   +   + PG + GP I K   ++I  LIESG KEGAK+   G      G+    FV PT+ +NV   M   KEEIFGPV   ++  ++DD IK  NN  YG    +FT     A   +  +  G V VN  +     M S     G F   G+    G+ G+  +T+LKTV 
Sbjct:   18 IQHTKIFINNEWHNSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPWRTMDASERGRLLNKLADLMERDRLLLATMEALNGGKVFANAYLSDLGGCIKALKYCAGWADKIHGQTIP---SDGDIFTYTRREPIGVCGQIIPWNFPMLMFIWKIGPALSCGNTVVVKPAEQTPLTALHLASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDIAVEFAHHGVFYHQGQCCVAASR-IFVEESVYDEFVKRSVERAKKYVLGNPLTPGINQGPQIDKEQHDKILDLIESGKKEGAKLECGGGRWGNKGF----FVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSVDDVIKRANNTTYGLAAGLFTKDLDKAITVSSALQAGVVWVNCYM-----MLSAQCPFGGFKMSGNGRELGEHGLYEYTELKTVA 493          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Match: Aldh1a3 (aldehyde dehydrogenase family 1, subfamily A3 [Source:MGI Symbol;Acc:MGI:1861722])

HSP 1 Score: 226.098 bits (575), Expect = 2.084e-66
Identity = 164/500 (32.80%), Postives = 249/500 (49.80%), Query Frame = 3
Query:   93 ALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES---WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVN-FICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYED-GNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFL--GDTNFYGKQGVNFFTQLKTVT 1553
            AL R   +  +  TK+FIN D+ +S++       NP+T + I  V +  KP++++AVE+   AF+    W +   L+R Q + +L +L++RD   +A   T + GK  L A    + G ++   +     +   G T+P    D ++V +   EP+GV   ITP+NFP ++  W    +L CGNT++LKP+E+ P   + L  L  E GFPPG VN++ G    V   I  HP+I  I+F G  + G+ + E  S+ N KRV   +G KN  ++  DA  +  +       F   GQ C A S  VFV E     ++   +  AK   V +      + GP I +   ++I  LIESG KEGAK+   G     S  ED G F+ PT+ ++V  +M   KEEIFGPV   L+   L++ IK  N+  YG   A+FT +   A K    +  G V +N         F      G F   G+    G+  +  +T++KTVT
Sbjct:   19 ALPRPIRNLEVKFTKIFINNDWHESKSGRKFATYNPSTLEKICEVEEGDKPDVDKAVEAAQAAFQRGSPWRRLDALSRGQLLHQLADLVERDRAILATLETMDTGKPFLHAFFVDLEGCIKTFRYFAGWADKIQGRTIP---TDDNVVCFTRHEPIGVCGAITPWNFPLLMLAWKLAPALCCGNTVVLKPAEQTPLTALYLASLIKEVGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVREAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASR-VFVEEQVYGEFVRRSVEFAKKRPVGDPFDAKTEQGPQIDQKQFDKILELIESGKKEGAKLECGG-----SAMEDRGLFIKPTVFSDVTDNMRIAKEEIFGPVQPILKFKNLEEVIKRANSTDYGLTAAVFTKNLDKALKLAAALESGTVWINC-----YNAFYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVT 504          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Match: sp|Q17M80|MMSA_AEDAE (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Aedes aegypti OX=7159 GN=AAEL001134 PE=3 SV=1)

HSP 1 Score: 772.311 bits (1993), Expect = 0.000e+0
Identity = 364/510 (71.37%), Postives = 437/510 (85.69%), Query Frame = 3
Query:   90 HALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +AL R+Y + ++P TKMFI+G FVDS+TTEWIDL +PATNKV++RVPKCT+ EME AVES  +AF++WSQ S+L RQQ MFKLQ LI+ ++ ++A NIT EQGKTL+DAEGDV+RGLQVVEHCCSIT+LQ+GET+P IAKD+D  SY  PLGVTAGI PFNFPAMIPLWMFP+++ CGNT I+KPSER PGATMMLMEL  EAG PPG VNVIHG HDAVNFICD+P I+A+SFVG D+AG++IYER  +NGKRVQ+N+GAKNHGV+M DA KE+T+NQL GAAFGAAGQRCMALSTAVFVGE + WIPD++ RA+ LKV+ G  PG D+GPVIS  +K+RI+ L+ESG KEGAK+VLDGR++KV  +E+GNFVGPTILT+V+ +M+CY EEIFGPVL+CL VDT+D+A+++INNNPYGNGTAIFT +GATARKF  +I VGQVGVNVPIPVPLPMFSFTGSRGSF+GD +FYGKQGV F+TQ KTVTQLWRE D  H    +A   PTM
Sbjct:   13 NALQRSYSTASVPTTKMFIDGKFVDSKTTEWIDLHDPATNKVVTRVPKCTQDEMESAVESSKKAFKTWSQTSILGRQQVMFKLQHLIRNNMSELAKNITKEQGKTLVDAEGDVLRGLQVVEHCCSITSLQMGETVPNIAKDMDTYSYTLPLGVTAGICPFNFPAMIPLWMFPVAITCGNTSIIKPSERVPGATMMLMELLNEAGCPPGVVNVIHGAHDAVNFICDNPTIKAVSFVGSDQAGKYIYERAGRNGKRVQSNMGAKNHGVIMADANKENTLNQLAGAAFGAAGQRCMALSTAVFVGEAKQWIPDLVERARKLKVNAGHVPGTDVGPVISPQSKQRINELVESGVKEGAKLVLDGRSIKVENFENGNFVGPTILTDVSTNMKCYTEEIFGPVLVCLTVDTVDEAVEMINNNPYGNGTAIFTTNGATARKFVNEIDVGQVGVNVPIPVPLPMFSFTGSRGSFMGDCHFYGKQGVKFYTQTKTVTQLWREGDVSHTK--AAVAMPTM 520          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Match: sp|Q7QC84|MMSA_ANOGA (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Anopheles gambiae OX=7165 GN=AGAP002499 PE=3 SV=2)

HSP 1 Score: 766.148 bits (1977), Expect = 0.000e+0
Identity = 359/510 (70.39%), Postives = 432/510 (84.71%), Query Frame = 3
Query:   90 HALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            + L R Y + ++P TKMFI+G FV+S+T +WIDL +PATN+V++RVPKCT+ EM+ AVES  +A+++W Q S+L+RQQ M KLQ +I+ ++ ++A NIT EQGKTL+DAEGDV+RGLQVVEHCCSIT+LQ+GET+P IAKD+D  SY  PLGVTAGI PFNFPAMIPLWMFP+++ CGNT I+KPSER PGATM+LME+  EAG PPG VNVIHG HDAVNF+CD+P IRA+SFVG D+AG++IYER  +NGKRVQ N+GAKNHGV+M DA KE+T+NQL GAAFGAAGQRCMALSTAVFVGE RNWIPD++ RA+ LKV+ G  PG DLGPVIS  +K+RI+ L+ESG KEGAKIVLDGRN+KV G+E GNFVGPTI+++V P+M+CY EEIFGPVL+CL VDT+D+AI+LINNNPYGNGTAIFT +GATARKF   I VGQVGVNVPIPVPLPMFSFTGSRGSFLGD +FYGKQG+ F+TQ KTVTQLWRE D  H    +A   PTM
Sbjct:   13 NVLQRGYSTASVPTTKMFIDGKFVESKTNDWIDLHDPATNEVVTRVPKCTQDEMQTAVESSKKAYKTWRQSSILSRQQVMLKLQHIIRNNMSELAKNITKEQGKTLIDAEGDVLRGLQVVEHCCSITSLQMGETVPNIAKDMDTYSYHLPLGVTAGIAPFNFPAMIPLWMFPVAITCGNTSIIKPSERVPGATMLLMEMLNEAGCPPGVVNVIHGAHDAVNFVCDNPDIRAVSFVGSDQAGKYIYERAGRNGKRVQCNMGAKNHGVIMADANKENTLNQLAGAAFGAAGQRCMALSTAVFVGEARNWIPDLVERARKLKVNAGHLPGTDLGPVISPQSKQRINELVESGAKEGAKIVLDGRNIKVEGFEKGNFVGPTIISDVTPNMKCYTEEIFGPVLVCLSVDTIDEAIELINNNPYGNGTAIFTTNGATARKFVNDIDVGQVGVNVPIPVPLPMFSFTGSRGSFLGDCHFYGKQGIKFYTQTKTVTQLWREGDVSHTK--AAVAMPTM 520          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Match: sp|Q9EQ20|MMSA_MOUSE (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Mus musculus OX=10090 GN=Aldh6a1 PE=1 SV=1)

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 344/499 (68.94%), Postives = 422/499 (84.57%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI+G FV+S++ +WID+ NPATN+V+ RVP+ TK EM+ AVESC  AF +W+  S+L+RQQ + + Q+LIK +LK+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQHDAVNFICDHP I+AISFVG ++AG +I+ERGS+NGKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTA+ VGE + W+P+++ RAKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV PSM CYKEEIFGPVL+ LE +TLD+AIK++N+NPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGRVPQSTKAEMDAAVESCKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHDAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVDRAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRRIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFGPVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGATARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 533          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Match: sp|Q02253|MMSA_RAT (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Rattus norvegicus OX=10116 GN=Aldh6a1 PE=1 SV=1)

HSP 1 Score: 746.117 bits (1925), Expect = 0.000e+0
Identity = 341/499 (68.34%), Postives = 419/499 (83.97%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI+G FV+S++ +WID+ NPATN+V+ RVP+ TK EME AV +C  AF +W+  S+L+RQQ + + Q+LIK +LK+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L LGETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQH+AVNFICDHP I+AISFVG ++AG +I+ERGS+NGKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++ RAKNL+V+ G QPGADLGP+I+  AKER+ +LI+SG KEGA I+LDGR +KV GYE+GNFVGPTI++NV PSM CYKEEIFGPVL+ LE +TLD+AIK++N+NPYGNGTAIFT +GA ARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGRVPQSTKAEMEAAVAACKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVERAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGAKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFGPVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGAIARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 533          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Match: sp|Q02252|MMSA_HUMAN (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Homo sapiens OX=9606 GN=ALDH6A1 PE=1 SV=2)

HSP 1 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 341/499 (68.34%), Postives = 419/499 (83.97%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            +P  K+FI G FV+S++ +WID+ NPATN+VI RVP+ TK EM+ A+ SC  AF +W+  SVL+RQQ + + Q+LIK +LK+IA  IT EQGKTL DAEGDV RGLQVVEH CS+T+L +GETMP I KD+D+ SY+ PLGV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER PGATM+L +L  ++G P GT+N+IHGQH+AVNFICDHP I+AISFVG +KAG +I+ERGS++GKRVQAN+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE + W+P+++  AKNL+V+ G QPGADLGP+I+  AKER+ +LI+SGTKEGA I+LDGR +KV GYE+GNFVGPTI++NV P+M CYKEEIFGPVL+ LE +TLD+AI+++NNNPYGNGTAIFT +GATARK+   + VGQVGVNVPIPVPLPMFSFTGSR SF GDTNFYGKQG+ F+TQLKT+T  W+EEDA   S   A   PTM
Sbjct:   37 VPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAFPAWADTSVLSRQQVLLRYQQLIKENLKEIAKLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMMGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEYIFERGSRHGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSS--PAVVMPTM 533          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Match: V5IF88 (Putative methylmalonate semialdehyde dehydrogenase (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 803.512 bits (2074), Expect = 0.000e+0
Identity = 374/508 (73.62%), Postives = 431/508 (84.84%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            L+R Y + A+P TKMFING FV+S+TTEW+DL NPATN++I+RVPKCT  EM  AV S  EAF +WSQ S+L RQQ MFKLQ+LIK ++K++AAN+T EQGKTL DAEGDV+RGLQVVEH C +T+LQLGET+P I KD+D +SY+ PLGVTAGITPFNFPAMIPLWMFPL+LVCGNTM+LKPSERDPG TMMLMEL  + G P G VNVIHG HDAV FICDHP IRAISFVG D+AG+ IYERGSKNGKRVQ N+GAKNHGV+MPDA KE T+NQL GAAFGAAGQRCMALSTAVFVGE +NWIP+++ RA+ LKV+ G  PGADLGPVIS  AKERI  L+ESG +EGAK++LDGR + V GYE GNFVGPT+L +V P M CY+EEIFGPVL+ L VD++DDAI LIN+NPYGNGTAIFT +GATARK+TQ+I VGQVGVNVPIPVPLPMFSFTGSRGSFLGD NFYGK GVNF+TQLKTVTQLWR +D+ H    +AT  P M
Sbjct:   14 LTRGY-AAAVPNTKMFINGQFVESKTTEWVDLKNPATNELITRVPKCTNAEMHEAVRSAKEAFRTWSQTSILTRQQSMFKLQQLIKENMKRLAANVTEEQGKTLADAEGDVLRGLQVVEHACGVTSLQLGETLPSITKDMDTMSYRIPLGVTAGITPFNFPAMIPLWMFPLALVCGNTMVLKPSERDPGCTMMLMELLKQTGVPDGVVNVIHGTHDAVKFICDHPDIRAISFVGSDQAGKFIYERGSKNGKRVQCNMGAKNHGVVMPDANKEYTLNQLTGAAFGAAGQRCMALSTAVFVGEAKNWIPELVERARKLKVNAGHVPGADLGPVISPEAKERICDLVESGVQEGAKLLLDGRGIVVPGYEKGNFVGPTVLADVTPQMRCYQEEIFGPVLLTLSVDSMDDAIALINSNPYGNGTAIFTTNGATARKYTQEIDVGQVGVNVPIPVPLPMFSFTGSRGSFLGDANFYGKAGVNFYTQLKTVTQLWRTQDSTHSK--AATAMPVM 518          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Match: A0A4D5RHE7 (Putative methylmalonate-semialdehyde dehydrogenase acylating mitochondrial (Fragment) OS=Ixodes scapularis OX=6945 PE=4 SV=1)

HSP 1 Score: 802.357 bits (2071), Expect = 0.000e+0
Identity = 373/508 (73.43%), Postives = 431/508 (84.84%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            L+R Y + A+P TKMFING FV+S+TTEW+DL NPATN++I+RVPKCT  EM  AV S  EAF +WSQ S+L RQQ MFKLQ+LIK ++K++AAN+T EQGKTL DAEGDV+RGLQVVEH C +T+LQLGET+P I KD+D +SY+ PLGVTAGITPFNFPAMIPLWMFPL+LVCGNTM+LKPSERDPG TMMLMEL  + G P G VNVIHG HDAV FICDHP IRAISFVG D+AG+ IYERGSKNGKRVQ N+GAKNHGV+MPDA KE T+NQL GAAFGAAGQRCMALSTAVFVGE +NWIP+++ RA+ LKV+ G +PGADLGPVIS  AKERI  L+ESG KEGAK++LDGR + + GYE GNFVGPT+L +V P M CY+EEIFGPVL+ L VD++DDAI LIN+NPYGNGTAIFT +GATARK+TQ+I V QVGVNVPIPVPLPMFSFTGSRGSFLGD NFYGK GVNF+TQLKTVTQLWR +D+ H    +AT  P M
Sbjct:   18 LTRGY-AAAVPNTKMFINGQFVESKTTEWVDLKNPATNELITRVPKCTNAEMHEAVRSAKEAFRTWSQTSILTRQQSMFKLQQLIKENMKRLAANVTEEQGKTLADAEGDVLRGLQVVEHACGVTSLQLGETLPSITKDMDTMSYRIPLGVTAGITPFNFPAMIPLWMFPLALVCGNTMVLKPSERDPGCTMMLMELLKQTGVPDGVVNVIHGTHDAVKFICDHPDIRAISFVGSDQAGKFIYERGSKNGKRVQCNMGAKNHGVVMPDANKEYTLNQLTGAAFGAAGQRCMALSTAVFVGEAKNWIPELVERARKLKVNAGHEPGADLGPVISPEAKERICDLVESGVKEGAKLLLDGRGIVMPGYEKGNFVGPTVLADVTPQMRCYQEEIFGPVLLTLSVDSMDDAIALINSNPYGNGTAIFTTNGATARKYTQEIDVRQVGVNVPIPVPLPMFSFTGSRGSFLGDANFYGKAGVNFYTQLKTVTQLWRTQDSTHSK--AATAMPVM 522          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Match: A0A131YMU9 (Methylmalonate-semialdehyde dehydrogenase OS=Rhipicephalus appendiculatus OX=34631 PE=4 SV=1)

HSP 1 Score: 800.431 bits (2066), Expect = 0.000e+0
Identity = 377/516 (73.06%), Postives = 433/516 (83.91%), Query Frame = 3
Query:   72 RLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            R TL      R Y S+++P TKMFING FV+S+TTEWIDL NPATN+ ++RVP CT  EME AV +  EAF++WSQ S+L RQQ MFKLQ LIK ++K+IAANIT EQGKTL DAEGDV+RGLQVVEH C +T+LQLGET+P I+KD+D +SY+ PLGV AGITPFNFPAMIPLWMFPL+LVCGNTM++KPSERDPGATM+LMEL  E G P G VNV HG H++V FICDHP IRAISFVG D AG+ IYERGSKNGKRVQ N+GAKNHGV+MPDA KE T+NQL GAAFGAAGQRCMALSTAVFVGE +NWIP+++ RAK L V+ G  PGADLGPVIS  AK+RI  L+ESG KEGAKI+LDGRN+KV G+E GNFVGPTILT+V PSM CY+EEIFGPVL+ L VDT+D+AI LIN+NPYGNGTAIFT +GATAR++TQ+I VGQVGVNVPIPVPLPMFSFTGSRGSFLGD NFYGK GVNF+TQLKTVTQLWR +D+ H     AT  P M
Sbjct:   11 RTTLLRRFPIRGYASSSVPTTKMFINGQFVESKTTEWIDLMNPATNEPVTRVPICTNDEMEAAVSAAKEAFKTWSQTSILTRQQSMFKLQNLIKENMKRIAANITEEQGKTLADAEGDVLRGLQVVEHACGVTSLQLGETLPSISKDMDTLSYRIPLGVCAGITPFNFPAMIPLWMFPLALVCGNTMVIKPSERDPGATMILMELLQECGVPNGVVNVFHGTHESVRFICDHPDIRAISFVGSDTAGKFIYERGSKNGKRVQCNMGAKNHGVIMPDANKEYTLNQLTGAAFGAAGQRCMALSTAVFVGEAKNWIPELVERAKKLNVNAGHVPGADLGPVISPEAKQRICDLVESGAKEGAKIILDGRNIKVPGFEKGNFVGPTILTDVKPSMRCYREEIFGPVLLTLNVDTMDEAISLINSNPYGNGTAIFTTNGATARRYTQEIDVGQVGVNVPIPVPLPMFSFTGSRGSFLGDNNFYGKAGVNFYTQLKTVTQLWRAQDSTHAK--PATAMPVM 524          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Match: A0A224YTE8 (Methylmalonate-semialdehyde dehydrogenase OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 799.66 bits (2064), Expect = 0.000e+0
Identity = 376/516 (72.87%), Postives = 433/516 (83.91%), Query Frame = 3
Query:   72 RLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            R TL      R Y S+++P TKMFING FV+S+TTEWIDL NPATN+ ++RVP CT  EME AV +  EAF++WSQ S+L RQQ MFKLQ LIK ++K+IAANIT EQGKTL DAEGDV+RGLQVVEH C +T+LQLGET+P ++KD+D +SY+ PLGV AGITPFNFPAMIPLWMFPL+LVCGNTM++KPSERDPGATM+LMEL  E G P G VNV HG H++V FICDHP IRAISFVG D AG+ IYERGSKNGKRVQ N+GAKNHGV+MPDA KE T+NQL GAAFGAAGQRCMALSTAVFVGE +NWIP+++ RAK L V+ G  PGADLGPVIS  AK+RI  L+ESG KEGAKI+LDGRN+KV G+E GNFVGPTILT+V PSM CY+EEIFGPVL+ L VDT+D+AI LIN+NPYGNGTAIFT +GATAR++TQ+I VGQVGVNVPIPVPLPMFSFTGSRGSFLGD NFYGK GVNF+TQLKTVTQLWR +D+ H     AT  P M
Sbjct:   11 RTTLLRRFPIRGYASSSVPTTKMFINGQFVESKTTEWIDLMNPATNEPVTRVPVCTNDEMEAAVSAAKEAFKTWSQTSILTRQQSMFKLQNLIKENMKRIAANITEEQGKTLADAEGDVLRGLQVVEHACGVTSLQLGETLPSVSKDMDTLSYRIPLGVCAGITPFNFPAMIPLWMFPLALVCGNTMVIKPSERDPGATMILMELLQECGVPNGVVNVFHGTHESVRFICDHPDIRAISFVGSDTAGKFIYERGSKNGKRVQCNMGAKNHGVIMPDANKEYTLNQLTGAAFGAAGQRCMALSTAVFVGEAKNWIPELVERAKKLNVNAGHVPGADLGPVISPEAKQRICDLVESGVKEGAKIILDGRNIKVPGFEKGNFVGPTILTDVKPSMRCYREEIFGPVLLTLNVDTMDEAISLINSNPYGNGTAIFTTNGATARRYTQEIDVGQVGVNVPIPVPLPMFSFTGSRGSFLGDNNFYGKAGVNFYTQLKTVTQLWRAQDSTHAK--PATAMPVM 524          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Match: A0A131Y2Q8 (Putative methylmalonate semialdehyde dehydrogenase (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 798.505 bits (2061), Expect = 0.000e+0
Identity = 373/508 (73.43%), Postives = 430/508 (84.65%), Query Frame = 3
Query:   96 LSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            L+R Y + A+P TKMFING FV+S+TTEW+DL NPATN++I+RVPKCT  EM  AV S  EAF +WSQ S+L RQQ MFKLQ+LIK ++K++AAN+T EQGKTL DAEGDV+RGLQVVEH C +T+LQLGET+P I KD+D +SY+ PLGVTA ITPFNFPAMIPLWMFPL+LVCGNTM+LKPSERDPG TMMLMEL  + G P G VNVIHG HDAV FICDHP IRAISFVG D+AG+ IYERGSKNGKRVQ N+GAKNHGV+MPDA KE T+NQL GAAFGAAGQRCMALSTAVFVGE +NWIP+++ RA+ LKV+ G  PGADLGPVIS  AKERI  L+ESG +EGAK++LDGR + V GYE GNFVGPT+L +V P M CY+EEIFGPVL+ L VD++DDAI LIN+NPYGNGTAIFT +GATARK+TQ+I VGQVGVNVPIPVPLPMFSFTGSRGSFLGD NFYGK GVNF+TQLKTVTQLWR +D+ H    +AT  P M
Sbjct:   14 LTRGY-AAAVPNTKMFINGQFVESKTTEWVDLKNPATNELITRVPKCTNAEMHEAVRSAKEAFRTWSQTSILPRQQSMFKLQQLIKENMKRLAANVTEEQGKTLADAEGDVLRGLQVVEHACGVTSLQLGETLPSITKDMDTMSYRIPLGVTACITPFNFPAMIPLWMFPLALVCGNTMVLKPSERDPGCTMMLMELLKQTGVPDGVVNVIHGTHDAVKFICDHPDIRAISFVGSDQAGKFIYERGSKNGKRVQCNMGAKNHGVVMPDANKEYTLNQLTGAAFGAAGQRCMALSTAVFVGEAKNWIPELVERARKLKVNAGHVPGADLGPVISPEAKERICDLVESGVQEGAKLLLDGRGIVVPGYEKGNFVGPTVLADVTPQMRCYQEEIFGPVLLTLSVDSMDDAIALINSNPYGNGTAIFTTNGATARKYTQEIDVGQVGVNVPIPVPLPMFSFTGSRGSFLGDANFYGKAGVNFYTQLKTVTQLWRTQDSTHSK--AATAMPVM 518          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Match: aldh1a2 (aldehyde dehydrogenase 1 family member A2 [Source:NCBI gene;Acc:103042804])

HSP 1 Score: 222.631 bits (566), Expect = 4.039e-65
Identity = 149/487 (30.60%), Postives = 243/487 (49.90%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN ++ DS + +     NPAT + I  V +  K ++++AV++   AF     W +    +R + + KL +L++RD   +A   + + GK  L +    ++G ++ + +     +   G ++P     L    + EP+GV   I P+NFP M+  W    +L CGNT+++KP+E+ P   + +  L  EAGFPPG VN++ G    A   I  H  I  ++F G  K G+ I E  G  N KRV   +G KN  ++  DA  +  + Q     F  +GQ C A  + +FV E+    ++   + RAK   V     P  + GP IS   + R+   +ESG  EGAK+   G+    + +  G ++ PT+ +NV   M   +EEIFGPV   ++  T+++ I+  NN  YG   A+FTN  + A   +  +  G V +N    +    P   F  S     G+    G+ G+  +T++KT+T
Sbjct:   36 IKHTKIFINNEWHDSLSGKTFSTYNPATGEKICDVQEADKADVDKAVKAARSAFSLSSEWRRMDSSDRGRLLSKLADLVERDRLYLATLESLDTGKPFLSSFFVDLQGTIKTLRYFAGWADKIHGTSIPTDGGYLSFTRH-EPIGVCGQIIPWNFPLMMMAWKLGPALCCGNTVVMKPAEQTPLTCLYMGSLIKEAGFPPGVVNILPGFGPTAGAGIASHMGIDKVAFTGSTKVGKLIQEAAGKSNLKRVTLELGGKNPNIIFADADLDLAVEQAHQGVFFNSGQCCTA-GSRIFVEESIYDEFVSRSVERAKRRIVGSPFDPTTEQGPQISAEQQRRVLEFVESGISEGAKLECGGK----AHFSKGFYIQPTVFSNVQDHMRIAREEIFGPVQQIMKFKTMEEVIERANNTEYGLTAAVFTNDISKAMAISTALQAGTVWINCFNALSCQCPFGGFKMS-----GNGRELGEYGLREYTEVKTIT 511          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Match: aldh1a2 (aldehyde dehydrogenase 1 family member A2 [Source:NCBI gene;Acc:103042804])

HSP 1 Score: 216.853 bits (551), Expect = 3.376e-63
Identity = 139/444 (31.31%), Postives = 225/444 (50.68%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
            I  TK+FIN ++ DS + +     NPAT + I  V +  K ++++AV++   AF     W +    +R + + KL +L++RD   +A   + + GK  L +    ++G ++ + +     +   G ++P     L    + EP+GV   I P+NFP M+  W    +L CGNT+++KP+E+ P   + +  L  EAGFPPG VN++ G    A   I  H  I  ++F G  K G+ I E  G  N KRV   +G KN  ++  DA  +  + Q     F  +GQ C A  + +FV E+    ++   + RAK   V     P  + GP IS   + R+   +ESG  EGAK+   G+    + +  G ++ PT+ +NV   M   +EEIFGPV   ++  T+++ I+  NN  YG   A+FTN  + A   +  +  G V +N
Sbjct:   36 IKHTKIFINNEWHDSLSGKTFSTYNPATGEKICDVQEADKADVDKAVKAARSAFSLSSEWRRMDSSDRGRLLSKLADLVERDRLYLATLESLDTGKPFLSSFFVDLQGTIKTLRYFAGWADKIHGTSIPTDGGYLSFTRH-EPIGVCGQIIPWNFPLMMMAWKLGPALCCGNTVVMKPAEQTPLTCLYMGSLIKEAGFPPGVVNILPGFGPTAGAGIASHMGIDKVAFTGSTKVGKLIQEAAGKSNLKRVTLELGGKNPNIIFADADLDLAVEQAHQGVFFNSGQCCTA-GSRIFVEESIYDEFVSRSVERAKRRIVGSPFDPTTEQGPQISAEQQRRVLEFVESGISEGAKLECGGK----AHFSKGFYIQPTVFSNVQDHMRIAREEIFGPVQQIMKFKTMEEVIERANNTEYGLTAAVFTNDISKAMAISTALQAGTVWIN 473          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Match: aldh1a3 (aldehyde dehydrogenase 1 family member A3 [Source:NCBI gene;Acc:103032207])

HSP 1 Score: 212.231 bits (539), Expect = 2.013e-61
Identity = 156/493 (31.64%), Postives = 244/493 (49.49%), Query Frame = 3
Query:  117 TAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLL-----DAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            T I  TK+FIN ++  S   + I + NPAT   I  V +  K +++ AVE+   A +   +W +  V  R + + KL EL++RD   +A   + + GK  L     D EG +    + + +     +   G+TMP +  +    +  EP+GV   I P+NFP ++  W    +L CGNT+++KPSE  P   + +  L  EAGFPPG VN++ G    +   I  H  I  ++F G  + G+ I E  +K N KRV   +G KN  ++  D+  +  + +    AF   GQ C A S  VFV E     ++   +  AK +   + M P    GP I++   E+I  L+ESG +EGAK+   G  L+    + G F+ PTI ++V   M   KEEIFGPV   ++  + ++ I+  N+  YG   A+FT S   A   +  +  G V VN    +    P   F G + S  G+    G+  +  +T++K VT
Sbjct:   27 TDIKHTKLFINNEWHSSAKGKTIPIFNPATGDKICEVEEADKADVDVAVEAARAAMKRGSAWRRMDVSGRGRLLHKLAELLERDRTLLATMESMDSGKPFLQSFFIDLEGSI----KTLRYYAGWADKIHGKTMP-VDDNFMCFTKHEPVGVCGAIIPWNFPLLMFTWKIAPALCCGNTVVIKPSELTPLTALHVGALIKEAGFPPGVVNIVPGFGPTSGAAIASHMGIDKVAFTGSVEVGQLIKEAAAKSNLKRVTLELGGKNPCIVFGDSDLQLAVQEAQKGAFYNQGQCCTAASR-VFVEERVYDEFVRLSVESAKKIVAGDPMDPRTSHGPQINQQQFEKILDLVESGKREGAKLECGGHALQ----DKGLFIQPTIFSDVKDHMRLAKEEIFGPVQCIMKFKSQEEVIERANSTHYGLAAAVFTRSVDRAMSVSSALEAGTVWVNCYNALHAQAP---FGGYKMS--GNGRELGEYALAEYTEVKAVT 504          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Match: aldh1a3 (aldehyde dehydrogenase 1 family member A3 [Source:NCBI gene;Acc:103032207])

HSP 1 Score: 209.149 bits (531), Expect = 2.000e-60
Identity = 153/493 (31.03%), Postives = 243/493 (49.29%), Query Frame = 3
Query:  117 TAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLL-----DAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            T +   ++FIN ++  S   + I + NPAT   I  V +  K +++ AVE+   A +   +W +  V  R + + KL EL++RD   +A   + + GK  L     D EG +    + + +     +   G+TMP +  +    +  EP+GV   I P+NFP ++  W    +L CGNT+++KPSE  P   + +  L  EAGFPPG VN++ G    +   I  H  I  ++F G  + G+ I E  +K N KRV   +G KN  ++  D+  +  + +    AF   GQ C A S  VFV E     ++   +  AK +   + M P    GP I++   E+I  L+ESG +EGAK+   G  L+    + G F+ PTI ++V   M   KEEIFGPV   ++  + ++ I+  N+  YG   A+FT S   A   +  +  G V VN    +    P   F G + S  G+    G+  +  +T++K VT
Sbjct:    2 TFLSNIQLFINNEWHSSAKGKTIPIFNPATGDKICEVEEADKADVDVAVEAARAAMKRGSAWRRMDVSGRGRLLHKLAELLERDRTLLATMESMDSGKPFLQSFFIDLEGSI----KTLRYYAGWADKIHGKTMP-VDDNFMCFTKHEPVGVCGAIIPWNFPLLMFTWKIAPALCCGNTVVIKPSELTPLTALHVGALIKEAGFPPGVVNIVPGFGPTSGAAIASHMGIDKVAFTGSVEVGQLIKEAAAKSNLKRVTLELGGKNPCIVFGDSDLQLAVQEAQKGAFYNQGQCCTAASR-VFVEERVYDEFVRLSVESAKKIVAGDPMDPRTSHGPQINQQQFEKILDLVESGKREGAKLECGGHALQ----DKGLFIQPTIFSDVKDHMRLAKEEIFGPVQCIMKFKSQEEVIERANSTHYGLAAAVFTRSVDRAMSVSSALEAGTVWVNCYNALHAQAP---FGGYKMS--GNGRELGEYALAEYTEVKAVT 479          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Match: aldh9a1b (aldehyde dehydrogenase 9 family member A1 [Source:NCBI gene;Acc:103023076])

HSP 1 Score: 209.534 bits (532), Expect = 2.589e-60
Identity = 127/462 (27.49%), Postives = 230/462 (49.78%), Query Frame = 3
Query:  195 NPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETR--NWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSG--YEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN----VPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             PAT +V+ ++  C   +++ AV+S   A+  W + + + R + M +   LI++  ++IA       GK++ +A  DV      +E+   +     G+   +PG +      + +EPLGV  GI  +N+P  I  W    ++ CGN+M+ KPS   P + ++L E+  +AG P G +NV+ G  +    +C HP +  +SF G    G+ I E  SK  K V   +G K+  ++  D+  E+ +   + A F + GQ C +  T VFV       ++ +++ R K +++ + +     +G ++S+   +++   +E   KEGA+++  G     +    ++G ++ P +L N    M C +EEIFGPV+  L  DT ++ ++  N+   G    +FT     A +  + +  G   +N     P+ VP     F G + S +G  N  G+  + F++QLKTV
Sbjct:   57 EPATGRVLCQLQSCKAGQVDEAVKSACSAYAVWRKTAGMERARVMLEAARLIEKRREEIAEVEVVNNGKSITEARLDVDSARLCIEYFAGLATNLSGQHVQLPGGSFAY---TRREPLGVCVGIGAWNYPFQIAAWKSAPAIACGNSMVFKPSPVTPVSAVLLAEIYAQAGAPEGLLNVVQGAQETGTLLCHHPSVAKVSFTGSVPTGKKIMEMASKGVKPVTLELGGKSPLIIFEDSDLENAVRGALMANFLSQGQVC-SNGTRVFVHRKVLPQFVTEVVRRTKAIQIGDPLLEETRMGALVSRPHLDKVLGYVEQAKKEGARVLCGGELFTPADTKLKNGYYMMPCVLDNCKDDMTCVREEIFGPVMSILTFDTEEEVLQRANDTTMGLAAGVFTKDIQRAHRVIENLQAGSCFINNYNITPVEVP-----FGGFKTSGIGREN--GQVTIEFYSQLKTV 507          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Match: aldh6a1 (aldehyde dehydrogenase 6 family, member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-9192])

HSP 1 Score: 712.22 bits (1837), Expect = 0.000e+0
Identity = 333/498 (66.87%), Postives = 399/498 (80.12%), Query Frame = 3
Query:  126 PKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            P TK+FI+G FVDS T+EW+D+ NPATN+V++ VPK        A ESC  A+  WS+ SVL RQQ   K Q+LIK ++K++A  IT EQGKTL DAEGDV RGLQVVEHCCSIT+L LGET+P IA+D+D  +Y+ PLGV AG+TPFNFPAMIPLWMFP++LVCGNT +LKPSER PG  M+L  L  +AG P GT+NV+HGQHDAV F+CDHP I+AISFVG ++AG +I+ RGS NGKRVQ N+GAKNHGV+M DA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE R W+P+++ RAKNL+V+ G QPGADLGP+IS  AK R+  L+ S  KEGA+I+LDGR + V GYE GNFVGPTI+T V P M+CY EEIFGPVL+ LE D+LD+A+ +IN+NPYGNGTAIFTN+GATARKFT ++ VGQ+G+NVPIPVPLPMFSFTGSRGSF GD NFYGKQG  F+TQLKTVT  W+ EDA         N PTM
Sbjct:    4 PTTKLFIDGKFVDSTTSEWLDVHNPATNEVVTCVPK--------AAESCQRAYGPWSETSVLTRQQVFLKYQQLIKDNIKELAKVITKEQGKTLADAEGDVFRGLQVVEHCCSITSLVLGETLPSIARDMDSYTYRLPLGVCAGVTPFNFPAMIPLWMFPVALVCGNTFLLKPSERVPGTAMLLTRLLQDAGAPDGTLNVVHGQHDAVKFVCDHPVIKAISFVGSNQAGEYIHTRGSANGKRVQCNMGAKNHGVVMADANKENTLNQLVGAAFGAAGQRCMALSTAVMVGEAREWLPELVERAKNLRVNAGDQPGADLGPLISPEAKARVCELVGSAEKEGARIILDGRKVSVKGYESGNFVGPTIITGVKPHMKCYTEEIFGPVLLVLEADSLDEAVSVINSNPYGNGTAIFTNNGATARKFTHEVDVGQIGINVPIPVPLPMFSFTGSRGSFRGDVNFYGKQGFQFYTQLKTVTAQWKAEDATESV--PTVNMPTM 491          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004609.1 (pep scaffold:Pmarinus_7.0:GL479321:13801:23388:1 gene:ENSPMAG00000004155.1 transcript:ENSPMAT00000004609.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 197.208 bits (500), Expect = 1.985e-56
Identity = 129/463 (27.86%), Postives = 222/463 (47.95%), Query Frame = 3
Query:  183 IDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE-SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGE--TMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNL--KVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
             D+ NPAT  ++ R       +++ AV+   EAFE SW   S   R Q++ +   +IK   +++A     + GK + +A  DV  G Q +E+   +     G+   +PG +    + S +EPLGV  GI  +NFP  I  W    +L CGN M+ KPS   P   + L E+  EAG PPG  N++ G  +    +C H  +  +S  G    G  + ++ +   K V   +G K+  ++  D   ++ +   +   F + GQ C + +T VFV  +    ++  ++ R   +++ +  +    +G +IS+   +R+ + ++   +EGAK+V  G+    +    + G F+ P +L      M+  +EEIFGPV+  L  DT ++ ++  N   +G    +FT     A +    +  G   +N    V + M  F G + S  G  N  G   V+++TQLKTV
Sbjct:   59 FDVVNPATGGLLCRGQASGAVDVQAAVKDAREAFEGSWRGLSGSERSQHLLRASAIIKERREELAVMEVKDTGKPVYEARMDVDSGWQCLEYFAGLAPTIAGQHIQLPGGSF---MYSRREPLGVCVGIGAWNFPFQIACWKSAPALACGNAMVFKPSPLTPLTAVALAEIYTEAGLPPGLFNIVQGDGETGQLLCSHNDVAKVSCTGSVGTGVKVLQQTAPRIKPVTLELGGKSPLIVFSDCDLDNAVKAALMGNFLSQGQVC-SNATRVFVQRSVCERFLERLLDRVSAIRIGDPQRDSTTMGALISQSHLDRVLAYVQRAVQEGAKLVCGGKKYVPEDPSLKGGFFMTPCVLAECRDDMQAVREEIFGPVMAVLAFDTEEEVLQRANATDFGLAAGVFTKDLTRAHRVIAALQAGSCYINNYNVVLMEM-PFGGYKMSGFGREN--GMATVDYYTQLKTV 514          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Match: aldh5a1 (aldehyde dehydrogenase 5 family, member A1 (succinate-semialdehyde dehydrogenase) [Source:ZFIN;Acc:ZDB-GENE-070228-2])

HSP 1 Score: 172.17 bits (435), Expect = 4.036e-48
Identity = 109/379 (28.76%), Postives = 184/379 (48.55%), Query Frame = 3
Query:  312 NRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV----NFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARA--KNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
             R   + +  E+I ++ +++A  IT+E GK   ++ G+V  G   +E          G+ +P  A D  + S  +P+GV A ITP+NFP  +       +L  G T ++KP+E  P + + L +L VEAG PPG +NV+      V      +C HP +  ISF G    G+ +        KRV   +G     ++   A  ++ +  L+ + F  +GQ C+  +  +      +     +A A    L+V  G   G  LGP+I++HA E++   +     +GA++V+ G    +     G+F  PT+L +V P+M C +EE FGP+   ++  T ++AI L N+   G     F+   A   + ++ + VG VG N
Sbjct:    2 ERGALLKRWYEVILQNRQELAQLITAESGKPQQESLGEVSYGAAFLEWFGEEARRAYGQIIPSAAPDRRMFSMYQPVGVAAMITPWNFPVAMITRKVGAALAAGCTAVVKPAEDTPLSALALAQLAVEAGLPPGVLNVVPCSRANVAAVGELLCTHPSVSKISFTGSTATGKVLMAHAVGTVKRVSMELGGHAPFIVFESADLDAAVRGLLSSKFRFSGQTCVCPNRMLVQESVHDAFVSRLAHAVSTQLRVGPGADTGNTLGPLINEHALEKVERHVVDAVSQGARVVVGGARHALG----GSFFQPTVLADVTPNMLCAREETFGPLAPIIKFKTEEEAIALANSTAMGLAGYFFSRDVAQVWRVSEALEVGMVGAN 376          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Match: aldh1l2 (aldehyde dehydrogenase 1 family, member L2 [Source:ZFIN;Acc:ZDB-GENE-100426-6])

HSP 1 Score: 173.711 bits (439), Expect = 6.778e-47
Identity = 134/498 (26.91%), Postives = 238/498 (47.79%), Query Frame = 3
Query:  105 NYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES--WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTL-LDAEGDVMRGLQVVEHCCSITNLQLGETMP-GIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV--NFICDHPKIRAISFVGGDKAGRHIYER-GSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEV--DTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            N L+  IP  + FI+G FVD+ T++  D  NP    VI +V   +  +++ AV +   AFES  W + +  +R + ++KL +L++   +++A   T + G    L  +  V   +Q   +     +   G T+P   A+    +++  +EP+GV   + P+N+P M+  W     L  GNT++LKP++  P   +   EL  +AGFP G +N+I G   ++    + +HP IR + F G    G+ I +   + N K+V   +G K+  ++  D   +  +   + A F   G+ C+A +  +FV E+    ++  ++   K +KV + +    D GP   +   E++    E G KEGA +V  GR +         F  P++ T V   M   +EE FGP+++  +     +D  +   NN  +G  + +FT   + A   + ++  G V +N      V  P   F G + S  G     G++ +N + + K VT
Sbjct:  202 NNLTVRIPH-QCFIDGKFVDADTSKTYDTVNPTDGSVICKVAFASVADVDEAVAAAKRAFESGEWGRMNPRDRGRLLYKLADLMEEHQEELATIETLDSGAVYTLALKTHVGMSIQTFRYFAGWCDKIHGTTIPINQARPNRNLTFTKREPIGVCGIVIPWNYPLMMLAWKSAACLAAGNTLVLKPAQVTPLTALKFAELAAKAGFPKGVINIIPGSVGSIVGQRMSEHPHIRKLGFTGSTPIGKQIMKSCATSNLKKVSLELGGKSPLIIFNDCDIDKAVRMGMSAVFFNKGENCIA-AGRLFVEESIHDEYVRRVVEEIKKMKVGDPLDRSTDHGPQNHRAHMEKLVEYCEVGVKEGATLVHGGRQVPRPEV----FANPSVFTGVEDHMYIAQEESFGPIMVISKFPDGDIDGVLARANNTEFGLASGVFTRDISKALYVSDQLEAGTVFINTYNKTDVAAP---FGGFKQSGFGKD--LGEEALNEYLRTKAVT 688          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Match: aldh8a1 (aldehyde dehydrogenase 8 family, member A1 [Source:NCBI gene;Acc:447801])

HSP 1 Score: 153.295 bits (386), Expect = 1.018e-40
Identity = 137/477 (28.72%), Postives = 220/477 (46.12%), Query Frame = 3
Query:  141 FINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQ-HDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIAR-AKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVL-DGRN--LKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            +ING+FV S+T   +D   P+T  V  RVP   + E+  AVE+   AF +W+ RS   R + + +L ELI+  L+++A   + +QGKT+  A   D+ R         S       E            + + P+GV   I+P+N P  +  W    ++  GNT++ KPSE       ML  L  EAG PPG VNV+ G    A   +  HP + AISF G       I  R + + KR+   +G KN  V+  DA  ++ +   V ++F        +  +A+F        P    R  K  KV       +++GP+ISK    ++   +E   ++G +++  +G +  L     + G F+ PT++T V       +EEIFGPV+     +  D+A+   N   YG    +++   A   +  +++  G V  N  +   L +  F G + S +G      K    FFT +KT+T
Sbjct:   13 YINGEFVASETH--VDSFEPSTGNVYCRVPDSGEAEVNAAVEAAKRAFPAWAARSPQERGRILTRLAELIESRLEELAIAESHDQGKTVEHARTVDIPRAAYNFRFFASAVQHYTTECTQADHVGCMHYTVRSPVGVVGLISPWNLPLYLLTWKIAPAIALGNTVVAKPSELTSVTAWMLCMLLREAGVPPGVVNVVLGTGQKAGAALVGHPDVPAISFTGSTATACDITVRSALHCKRLSLELGGKNPAVIFADADLDACVATTVRSSFANQTGHVPSSLSAIFSHSVVVQCPRRFFRTTKAWKVGPPDDFTSNMGPLISKEHLAKVRGYVELAERDGGQVLCGEGVDPLLLPPRCQGGYFLAPTVITGVPDDSPAMQEEIFGPVVCVSPFEDEDEAVTRSNGVRYGLAAVVWSRDVARVHRVAKRLQAGIVWTNCWLIRELNL-PFGGMKASGIGREG--AKDSFEFFTDVKTIT 484          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Match: ALD4 (Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant [Source:SGD;Acc:S000005901])

HSP 1 Score: 209.534 bits (532), Expect = 3.536e-61
Identity = 125/438 (28.54%), Postives = 227/438 (51.83%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE--SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV-NFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
            T +FIN  FV S+  +  ++ NP+T + I  + +  + ++E AV++   AF   SW+    ++R + +++L ELI++D   IA+  T + GK +  + GDV   +  ++      + ++   M    +     + ++PLGV   I P+NFP ++  W    +LV GNT++LK +E  P + + + +   +AG PPG +N++ G    V   I +HPKI+ ++F G    GRHIY+  +   K+V   +G K+  ++  DA  +  +  ++   +  +G+ C A  + V+V E+    +I +  A ++++KV +        G   S+    +I   ++ G  EGA ++  G  L   GY    F+ PT+  +V   M   KEEIFGPV+   +  + D+ I + N++ YG    I T++  TA K   +++ G V +N
Sbjct:   45 TGLFINNKFVPSKQNKTFEVINPSTEEEICHIYEGREDDVEEAVQAADRAFSNGSWNGIDPIDRGKALYRLAELIEQDKDVIASIETLDNGKAISSSRGDVDLVINYLKSSAGFAD-KIDGRMIDTGRTHFSYTKRQPLGVCGQIIPWNFPLLMWAWKIAPALVTGNTVVLKTAESTPLSALYVSKYIPQAGIPPGVINIVSGFGKIVGEAITNHPKIKKVAFTGSTATGRHIYQSAAAGLKKVTLELGGKSPNIVFADAELKKAVQNIILGIYYNSGEVCCA-GSRVYVEESIYDKFIEEFKAASESIKVGDPFDESTFQGAQTSQMQLNKILKYVDIGKNEGATLITGGERLGSKGY----FIKPTVFGDVKEDMRIVKEEIFGPVVTVTKFKSADEVINMANDSEYGLAAGIHTSNINTALKVADRVNAGTVWIN 476          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Match: ALD5 (Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed [Source:SGD;Acc:S000000875])

HSP 1 Score: 207.994 bits (528), Expect = 1.169e-60
Identity = 128/437 (29.29%), Postives = 218/437 (49.89%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES-WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV-NFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
            T +FING+FV S+  +  D+ NP+  + I+ V K  + +++ AV +  +AFE+ WS      R + +F L +L+++  + +AA  + + GK+L  A GDV    + +  C    +   G  +    K+    S KEPLGV   I P+NFP ++  W    +L  GNT++LKP+E  P + +   +LC EAG P G VN++ G    V   +  HP ++ I+F G    GRHI +  +   K+V   +G K+  ++  DA  +  +  +    F  +G+ C A  + +++ +T     +  +    ++LKV +        G   S     +I   ++    EGA++V  G      GY    FV PT+  +V   M   KEE+FGP++   +  T+D+ I + N++ YG    I TN    A   ++++  G V +N
Sbjct:   44 TGLFINGEFVASKQKKTFDVINPSNEEKITTVYKAMEDDVDEAVAAAKKAFETKWSIVEPEVRAKALFNLADLVEKHQETLAAIESMDNGKSLFCARGDVALVSKYLRSCGGWADKIYGNVID-TGKNHFTYSIKEPLGVCGQIIPWNFPLLMWSWKIGPALATGNTVVLKPAETTPLSALFASQLCQEAGIPAGVVNILPGSGRVVGERLSAHPDVKKIAFTGSTATGRHIMKVAADTVKKVTLELGGKSPNIVFADADLDKAVKNIAFGIFYNSGEVCCA-GSRIYIQDTVYEEVLQKLKDYTESLKVGDPFDEEVFQGAQTSDKQLHKILDYVDVAKSEGARLVTGGARHGSKGY----FVKPTVFADVKEDMRIVKEEVFGPIVTVSKFSTVDEVIAMANDSQYGLAAGIHTNDINKAVDVSKRVKAGTVWIN 474          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Match: ALD2 (Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p [Source:SGD;Acc:S000004780])

HSP 1 Score: 200.675 bits (509), Expect = 4.597e-58
Identity = 152/504 (30.16%), Postives = 240/504 (47.62%), Query Frame = 3
Query:  108 YLSTAIPKTK--------MFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES-WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTL-LDAEGDVMRGLQVVEHCC-SITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV-NFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKN-LKVSEGMQPGAD---LGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVP----IPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
            Y    IP+ K        +FIN +F  S   + I+  NPAT + I+      + ++++AV++   AF++ WS+ S   R  Y+  L +LI+ +   +AA  T + GK    +A+GD+ + LQ+  +   S      G T+P +  +    + K P GV A I P+N+P  +  W    +L  GNT+I+KP+E    + +    L  +AGFPPG VN++ G    V   +  H  I  ISF G  K G  + E  G  N K V    G K+  ++  DA  +  I+ +    F  +GQ C A ++ V+V  +    ++      AK    V+    P  +   +GPVIS    +RI S IE G +E    +       + G + G F+ PTI T+V  + +  ++EIFGPV++  +    DDA+KL N+  YG  +A+FT     A  F + I  G V +N      + VP   F  +G            G+ GV+ + Q K V
Sbjct:    5 YTDIEIPQLKISLKQPLGLFINNEFCPSSDGKTIETVNPATGEPITSFQAANEKDVDKAVKAARAAFDNVWSKTSSEQRGIYLSNLLKLIEEEQDTLAALETLDAGKPYHSNAKGDLAQILQLTRYFAGSADKFDKGATIP-LTFNKFAYTLKVPFGVVAQIVPWNYPLAMACWKLQGALAAGNTVIIKPAENTSLSLLYFATLIKKAGFPPGVVNIVPGYGSLVGQALASHMDIDKISFTGSTKVGGFVLEASGQSNLKDVTLECGGKSPALVFEDADLDKAIDWIAAGIFYNSGQNCTA-NSRVYVQSSIYDKFVEKFKETAKKEWDVAGKFDPFDEKCIVGPVISSTQYDRIKSYIERGKREEKLDMFQTSEFPIGGAK-GYFIPPTIFTDVPQTSKLLQDEIFGPVVVVSKFTNYDDALKLANDTCYGLASAVFTKDVKKAHMFARDIKAGTVWINSSNDEDVTVPFGGFKMSGIGREL-------GQSGVDTYLQTKAV 498          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Match: ALD3 (Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose [Source:SGD;Acc:S000004779])

HSP 1 Score: 193.356 bits (490), Expect = 2.875e-55
Identity = 153/504 (30.36%), Postives = 243/504 (48.21%), Query Frame = 3
Query:  108 YLSTAIPKTK--------MFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES-WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTL-LDAEGDVMRGLQVVEHCC-SITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAV-NFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKN-LKVSEGMQPGAD---LGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV----PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV 1550
            Y    IP+ K        +FIN +F  S   + I+  NPAT + I+      + ++++AV++   AF++ WS+ S   R  Y+  L +LI+ +   +AA  T + GK    +A+ D+ + +++  +   ++    +GET+P +  +    + K P GV A I P+N+P  +       +L  GNT+I+KP+E    + +    L  +AGFPPG VNVI G    V   +  H  I  ISF G  K G  + E  G  N K +    G K+  ++  DA  +  I  +    F  +GQ C A ++ V+V  +    ++      AK    V+    P  +   +GPVIS    +RI S IE G KE    +       + G + G F+ PTI T+V  + +  ++EIFGPV++  +    DDA+KL N+  YG  +A+FT     A  F + I  G V +N        VP   F  +G  G   GDT      GV+ + Q+K+V
Sbjct:    5 YTDIEIPQLKISLKQPLGLFINNEFCPSSDGKTIETVNPATGEPITSFQAANEKDVDKAVKAARAAFDNVWSKTSSEQRGIYLSNLLKLIEEEQDTLAALETLDAGKPFHSNAKQDLAQIIELTRYYAGAVDKFNMGETIP-LTFNKFAYTLKVPFGVVAQIVPWNYPLAMACRKMQGALAAGNTVIIKPAENTSLSLLYFATLIKKAGFPPGVVNVIPGYGSVVGKALGTHMDIDKISFTGSTKVGGSVLEASGQSNLKDITLECGGKSPALVFEDADLDKAIEWVANGIFFNSGQICTA-NSRVYVQSSIYDKFVEKFKETAKKEWDVAGKFDPFDEKCIVGPVISSTQYDRIKSYIERGKKEEKLDMFQTSEFPIGGAK-GYFIPPTIFTDVPETSKLLRDEIFGPVVVVSKFTNYDDALKLANDTCYGLASAVFTKDVKKAHMFARDIKAGTVWINQTNQEEAKVPFGGFKMSGI-GRESGDT------GVDNYLQIKSV 498          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Match: ALD6 (Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress [Source:SGD;Acc:S000005982])

HSP 1 Score: 185.652 bits (470), Expect = 1.450e-52
Identity = 123/442 (27.83%), Postives = 214/442 (48.42%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFES--WSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNF-ICDHPKIRAISFVGGDKAGRHI-YERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAK-----NLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
            T +FIN  F+ +Q  +   + +P+T   +  V   T  ++E A+E    AF    W+ +    R + + KL + ++  +  +++    + GKTL  A GDV   +  +    +  +   G T+      ++  +  EP+GV   I P+NFP M+  W    +L  GN  ILKP+   P   +    LC + G P G VN++ G    V   + + P+IR ++F G  + G+ +  +    N K++   +G K+  ++  DA  + T+  LV   F  AGQ C +  + ++V E      +++A  K      +KV          G + ++   + I + I+ G KEGAKI+  G  +   GY    F+ PT+  +VN  M   KEEIFGPV+   +  TL++ +++ N++ +G G+ I T S +T  K  + +  G V +N
Sbjct:   26 TGLFINNKFMKAQDGKTYPVEDPSTENTVCEVSSATTEDVEYAIECADRAFHDTEWATQDPRERGRLLSKLADELESQIDLVSSIEALDNGKTLALARGDVTIAINCLRDAAAYADKVNGRTINTGDGYMNFTTL-EPIGVCGQIIPWNFPIMMLAWKIAPALAMGNVCILKPAAVTPLNALYFASLCKKVGIPAGVVNIVPGPGRTVGAALTNDPRIRKLAFTGSTEVGKSVAVDSSESNLKKITLELGGKSAHLVFDDANIKKTLPNLVNGIFKNAGQICSS-GSRIYVQE--GIYDELLAAFKAYLETEIKVGNPFDKANFQGAITNRQQFDTIMNYIDIGKKEGAKILTGGEKVGDKGY----FIRPTVFYDVNEDMRIVKEEIFGPVVTVAKFKTLEEGVEMANSSEFGLGSGIETESLSTGLKVAKMLKAGTVWIN 459          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Match: EDO49572 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGP1])

HSP 1 Score: 748.429 bits (1931), Expect = 0.000e+0
Identity = 348/506 (68.77%), Postives = 426/506 (84.19%), Query Frame = 3
Query:  102 RNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            + Y S  IP TKMFING F DS++++WIDL NPATN+VI+RVP+CT+ EM+ AV++ A AF SWS+ SVL RQQ MF LQ LIK+++K++A NIT EQGKTL DAEGDV+RGLQVVEH CSIT+LQ+GETMP + KD+D  SY+ PLGV AGITPFNFPAMIPLWMFP+++VCGNT ++KPSERDPGATMML+++  +AG P G VN IHG  +AV FIC++P I+AISFVG D+AG++IYE GSK+GKRVQ+N+GAKNHGV+MPDA KE+TINQLVGAAFGAAGQRCMALSTA+FVGET+ WIP+I+ +AK LKVS G QPGADLGP+IS  AK+R   L++SG ++GA + LDGR++ V GYE+GNFVGPT+L+NV P M CYKEEIFGPVL+ L  DTL+DA+++IN NPYGNGTAIFT SGA ARKF QK +VGQ+G+NVPIPVPLPMFSFTGSRGSFLGD++FYGKQG+NF+T  KT+T LWR+ED+    +    NFP M
Sbjct:   26 KRYSSDGIPTTKMFINGQFSDSKSSKWIDLHNPATNEVITRVPECTQDEMQAAVDAAAAAFLSWSESSVLTRQQIMFNLQHLIKKNMKELAKNITLEQGKTLADAEGDVLRGLQVVEHTCSITSLQMGETMPSVTKDMDTYSYRVPLGVCAGITPFNFPAMIPLWMFPMAMVCGNTYVIKPSERDPGATMMLLKMAQDAGVPDGVVNCIHGAREAVKFICENPTIKAISFVGSDQAGKYIYETGSKHGKRVQSNMGAKNHGVIMPDANKENTINQLVGAAFGAAGQRCMALSTAIFVGETKEWIPEIVEKAKQLKVSAGDQPGADLGPMISPQAKKRACDLVQSGVEQGASLDLDGRDIVVKGYENGNFVGPTVLSNVTPEMTCYKEEIFGPVLVALNADTLEDAVEIINRNPYGNGTAIFTKSGAIARKFAQKANVGQIGINVPIPVPLPMFSFTGSRGSFLGDSHFYGKQGINFYTGTKTITSLWRDEDSV--PQKVQANFPIM 529          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Match: EDO45538 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB4])

HSP 1 Score: 217.624 bits (553), Expect = 5.995e-64
Identity = 154/484 (31.82%), Postives = 237/484 (48.97%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            I  TK+FIN +FVD  + +     NP T + I  + +  K ++++AV++  EAF+   +W       R + ++KL +LI RD+  +A+  T + GK   D+ GD+        +     +   G+T+P       + +  EP+G+   ITP+NFP  +       +L CGN +ILKP+E+ P   +    L  EAGFP G VNVI G    A   I  H  I  +SF G  + GR I E  +K N KR+   +G K+  ++  D+  +  +     A F   GQ C A S   FV +T    ++   + RAK   V +      + GP I +   ++I  LIESG KEGAK+   G      G+    F+ PT+ ++V   M   KEEIFGPV   ++  ++D+ I+  NN  YG    +FT +  TA   +  +  G V +N       P   F G + S  G    +G+ GV  + ++KTVT
Sbjct:   13 IKYTKLFINNEFVDCTSGKVFPTINPTTGEKICDISEGDKEDVDKAVKAAKEAFKLGSAWRTMDASMRGKLLYKLAQLIDRDIAYLASLETIDSGKLFSDSVGDMQSSANCFRYYAGWADKVTGKTIPADGPYF-VYTRHEPVGLVGAITPWNFPLNMASVKIAPALACGNVVILKPAEQTPLTALYFCALVKEAGFPAGVVNVIPGYGPTAGAAITTHLDIDKVSFTGSTEVGRLIQEASAKSNLKRLTLELGGKSPNIVFADSDMDYAVEMAHEALFFNQGQCCSAGSR-TFVQDTIYDEFVKKSVKRAKARTVGDPFD-SVEQGPQIDQEQFDKIMDLIESGKKEGAKMQCGGARHGNKGF----FIQPTVFSDVTDDMRIAKEEIFGPVQQLIKFKSVDEVIERANNTTYGLAAGVFTKNIDTAIAVSSGLRAGTVWINC-YECGAPQAPFGGYKMSGYGRE--WGEYGVLPYCEVKTVT 486          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Match: EDO36183 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJA5])

HSP 1 Score: 196.823 bits (499), Expect = 1.371e-56
Identity = 142/440 (32.27%), Postives = 216/440 (49.09%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVG 1415
            +  T++FIN +FVDS + +     NPAT + I  V +  K +++ AV +  EAF+    W       R  +++KL +L +RD   +A   + + GK + +A+ DV   +    +     +   G+T+P    D    +Y   EP+GV   ITP+NFP ++       +L CG  +ILKP+E+ P   + L  L  EAGFP G VNV+ G    A   I  H  I  ISF G  + GR I E  +K N KRV   +G K+  ++  DA  +  +       F   GQ C A  T +FV E+    ++   +ARAK   V + ++     GP + +    +I  LIESG ++GAK+   G      GY    FV PT+ + V   M   KEEIFGPV+  L+ + +++ I+  N+  YG   A+F+     A      I  G
Sbjct:    6 VKYTQLFINNEFVDSVSGKTFPTLNPATEEKICDVSEADKADVDIAVAAAKEAFKLGSVWRTMDASARGHFLYKLADLCERDADYLARLESYDGGKVINEAKIDVQGMISCFRYYAGWADKVTGKTIPA---DGPFFTYTRHEPVGVVGAITPWNFPLLMEGLKLAPALACGCVVILKPAEQTPLTALYLASLVKEAGFPTGVVNVLPGYGPTAGAAISSHMGIDKISFTGSTEVGRLIQETAAKSNLKRVTLELGGKSPNIVFADADLDWAVEMSCLGLFINQGQCCCA-GTRIFVEESIHDEFVRQCVARAKARVVGDPLEEKTVQGPQVDEEQFNKILGLIESGKQQGAKVECGGGRHGNKGY----FVEPTVFSGVQDDMRIAKEEIFGPVMQILKFNEINEVIRRANDTTYGLAGAVFSKDIDKALMVAHSIQAG 437          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Match: EDO47653 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLS5])

HSP 1 Score: 195.282 bits (495), Expect = 1.813e-55
Identity = 144/487 (29.57%), Postives = 239/487 (49.08%), Query Frame = 3
Query:  123 IPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYER--GSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALS-TAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            I   ++FIN  FV+S + +     NP+T +VI  V +  K +++ AV++   AF+    W      +R + + +L +LI+RD   +A+  T + GK   D+   D+   ++   +     +   G+T+P +       +  EP+GV   + P+NFP ++  W    +L CGNT+++KP+E+ P   + +  L  EAGFP G VN++ G    A   I  H  +  I+F G  + G HI ++  G+ N K V   +G K+  +++ D+  +  ++    A F   GQ C A S T V  G    ++   + RAKN  V          GP +      +I +LIESG KEGAK+ + G      GY    F+ PT+ ++V  +M   +EEIFGPV+       ++D I+  N   YG   ++ T +   A   +  + VG V VN    +    P   F G + S +G     G+ G+  ++++KTVT
Sbjct:   40 IKYNQVFINNQFVNSVSGKTFQTFNPSTGEVICDVAEGDKHDVDIAVKAAQSAFKLGSPWRTMDASDRGRLLNRLADLIERDKAYLASLETLDNGKPYNDSFNVDLEFTIKCYRYYAGWADKIHGKTIP-LDGSFFCYTRHEPVGVVGQVIPWNFPLLMQAWKLGPALACGNTVVMKPAEQTPLTALYVASLIAEAGFPEGVVNIVPGFGPTAGGAIASHMDVDKIAFTGSTEVG-HIIQQSAGASNLKNVTLELGGKSPNIVLADSDVDFAVDMSHFALFFNQGQCCCAGSRTFVQEGIYDEFVEKSVQRAKNRVVGNPFDLKTQQGPQVDGEQMTKILNLIESGRKEGAKLEVGGDRAGDKGY----FIQPTVFSDVQDNMRIAQEEIFGPVMQIFRFKDMNDIIERANKTTYGLAGSVMTKNLENALMLSNSLRVGTVWVNCYDALSAQAP---FGGYKMSGVGRE--LGEYGLQQYSEVKTVT 515          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Match: EDO34362 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPJ4])

HSP 1 Score: 193.356 bits (490), Expect = 2.926e-55
Identity = 128/465 (27.53%), Postives = 216/465 (46.45%), Query Frame = 3
Query:  189 LPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKV-SGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN----VPIPVPLPMFSFTGSRGSFLGDTNFYGKQG----VNFFTQLKTV 1550
            + +P+T  +   V    K E++RAV S  +AF++WS  S   R + +     L+++  + IA     + GK   +A  DV     V+E+   +     G+ +  +       + +EPLGV  GI  +N+P     W    ++ CGNT++ KPS   P   + L ++  EAG PPG VN+I G  +    +   P +  +SF G    G+ I    +   K V   +G K+  ++  DA   + ++  + A F + GQ C +  T VFV ++    ++  ++ R KN++V + M P   +G +IS     ++   ++ G  EGAK++  G  L +      G ++ P ++      M   KEEIFGPV+  L   T ++A+   NN  +G    IFT     A +    +  G   +N     P+ VP   +  +G           +G++G    V  +TQLKTV
Sbjct:    3 ITDPSTGHISCEVKGSGKTEVKRAVLSARKAFKTWSVLSGSERGRILGDASRLVRKRREDIAKVEVHDNGKPFHEALWDVDNVADVLEYYAGVAPTLSGQHVQ-LPNGSFAYTRREPLGVVGGIGAWNYPIQTACWKIAPAIACGNTIVYKPSPLTPMNAVALADILTEAGVPPGVVNIIQGGGETGEMLSKDPNVAKVSFTGSVPTGQKIMASCADGIKHVTLELGGKSPLIIFKDADMANAVSGALMANFLSQGQVC-SNGTRVFVEDSIKDEFLEKVVERTKNIRVGDPMAPDTQMGAMISSAHLNKVLDYVQIGKDEGAKVLCGGERLHLPEPLTSGYYMSPCVMAECTDDMTVVKEEIFGPVMTVLTFQTEEEAVTRANNTTFGLAGGIFTRDLNRAHRVIGSLQAGTCWINSFNITPVEVPFGGYKMSG-----------FGREGGTAAVEHYTQLKTV 454          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Match: aldh6a1 (aldehyde dehydrogenase 6 family member A1 [Source:NCBI gene;Acc:101161931])

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 347/502 (69.12%), Postives = 417/502 (83.07%), Query Frame = 3
Query:  114 STAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            S+++P  K+FI+G FV+S+T+EW+++ NPATN+VI+RVPK T  EM  A ESC+ +F SWS+ S L RQQ   + Q+LIK ++K++A  IT EQGKTL DAEGDV RGLQVVEH CSIT+L LGET+P I KD+D  +Y+ PLGV AGI PFNFPAMIPLWMFP+ +VCGNT +LKPSER P  TM+L +L  +AG P GT+N+IHGQHDAVNFICDHP I+AISFVG ++AG +IYERGS+NGKRVQ+N+GAKNHGV+MPDA KE+T+NQLVGAAFGAAGQRCMALSTAV VGE RNW+P+++ R K L+V+ G QPGADLGP+IS  AKER+ SLI+SG  EGAK+VLDGRN+KV GYE+GNFVGPTI++ V P M+CY EEIFGPVLI LE DTLDDAI+L+N NPYGNGTAIFT +GATARK+T ++ VGQVGVNVPIPVPLPMFSFTGSRGSF GDTNFYGKQG+ F+TQ+KTVT  W+ EDA    K  A   PTM
Sbjct:   24 SSSVPTVKLFIDGKFVESKTSEWLEIHNPATNEVIARVPKATHEEMLAAAESCSRSFHSWSETSALTRQQVFLRYQQLIKDNIKELAKAITVEQGKTLADAEGDVFRGLQVVEHACSITSLMLGETLPSITKDMDTYTYRLPLGVCAGIAPFNFPAMIPLWMFPMGMVCGNTYLLKPSERVPTCTMLLAKLLQDAGAPDGTLNIIHGQHDAVNFICDHPAIKAISFVGSNQAGEYIYERGSRNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEARNWLPELVERTKALRVNAGDQPGADLGPLISPQAKERVCSLIQSGVDEGAKLVLDGRNVKVKGYENGNFVGPTIISGVTPEMKCYTEEIFGPVLIVLEADTLDDAIRLVNKNPYGNGTAIFTTNGATARKYTHEVDVGQVGVNVPIPVPLPMFSFTGSRGSFRGDTNFYGKQGIQFYTQIKTVTSQWKAEDATL--KSPAVTMPTM 523          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Match: ENSORLT00000045168.1 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Source:NCBI gene;Acc:101158320])

HSP 1 Score: 749.199 bits (1933), Expect = 0.000e+0
Identity = 343/502 (68.33%), Postives = 421/502 (83.86%), Query Frame = 3
Query:  114 STAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            S+++P TK+FI+G FV+S ++EW+D+ NPATN+V+ RVPK T+ EM  AV+SC+ +F+SWS+ S+L RQQ   + Q+LIK ++K+++  IT EQGKTL DAEGDV RGLQVVEH CSIT+L LGET+P I KD+D  +Y+ P+GV AGI PFNFPAMIPLWMFP+ +VCGNT ++KPSER PG TM+L +L  +AG P GT+N+IHGQH AVNFICDHP I+AISFVG ++AG +IYERGSKNGKRVQ+N+GAKNHGV+MPDA KE+TINQLVGAAFGAAGQRCMALSTA+FVGE+R+W+P+++ R+K L V+ G QPGAD+GP+IS  AK R+  LI+SG  EGAK++LDGRN++V GYE+GNFVGPT+LTNV+P M CYKEEIFGPVLI LEVDTLD+AI LIN NPYGNGTAIFT +GATARK+T  + VGQ+GVNVPIPVPLPMFSFTGSRGSF GDTNFYGKQG+ F+TQ+KTVT  W+ EDA   S   A   PTM
Sbjct:   26 SSSVPTTKLFIDGKFVESNSSEWLDVHNPATNQVVGRVPKATQEEMLAAVDSCSRSFQSWSETSILTRQQVFLRYQQLIKDNIKELSKIITLEQGKTLADAEGDVFRGLQVVEHACSITSLMLGETLPSITKDMDTYTYRMPIGVCAGIAPFNFPAMIPLWMFPIGMVCGNTYLMKPSERVPGCTMLLAKLLQDAGAPDGTLNIIHGQHAAVNFICDHPAIKAISFVGSNQAGEYIYERGSKNGKRVQSNMGAKNHGVVMPDANKENTINQLVGAAFGAAGQRCMALSTAIFVGESRDWLPELVERSKLLHVNAGDQPGADVGPLISPEAKARVEHLIQSGVDEGAKLLLDGRNVRVKGYENGNFVGPTVLTNVSPEMTCYKEEIFGPVLIVLEVDTLDEAIALINRNPYGNGTAIFTTNGATARKYTHGVDVGQIGVNVPIPVPLPMFSFTGSRGSFRGDTNFYGKQGIQFYTQIKTVTSQWKAEDATVSSP--AVTMPTM 525          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Match: ENSORLT00000011823.2 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Source:NCBI gene;Acc:101158320])

HSP 1 Score: 457.218 bits (1175), Expect = 9.411e-158
Identity = 207/308 (67.21%), Postives = 257/308 (83.44%), Query Frame = 3
Query:  120 AIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNL 1043
            AIP TK+FI+G FV+S ++EW+D+ NPATN+V+ RVPK T+ EM  AV+SC+ +F+SWS+ S+L RQQ   + Q+LIK ++K+++  IT EQGKTL DAEGDV RGLQVVEH CSIT+L LGET+P I KD+D  +Y+ P+GV AGI PFNFPAMIPLWMFP+ +VCGNT ++KPSER PG TM+L +L  +AG P GT+N+IHGQH AVNFICDHP I+AISFVG ++AG +IYERGSKNGKRVQ+N+GAKNHGV+MPDA KE+TINQLVGAAFGAAGQRCMALSTA+FVGE+R+W+PD      NL
Sbjct:   71 AIPTTKLFIDGKFVESNSSEWLDVHNPATNQVVGRVPKATQEEMLAAVDSCSRSFQSWSETSILTRQQVFLRYQQLIKDNIKELSKIITLEQGKTLADAEGDVFRGLQVVEHACSITSLMLGETLPSITKDMDTYTYRMPIGVCAGIAPFNFPAMIPLWMFPIGMVCGNTYLMKPSERVPGCTMLLAKLLQDAGAPDGTLNIIHGQHAAVNFICDHPAIKAISFVGSNQAGEYIYERGSKNGKRVQSNMGAKNHGVVMPDANKENTINQLVGAAFGAAGQRCMALSTAIFVGESRDWLPDSYGSFLNL 378          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Match: aldh1a2 (aldehyde dehydrogenase 1 family member A2 [Source:NCBI gene;Acc:100049450])

HSP 1 Score: 217.624 bits (553), Expect = 2.931e-63
Identity = 152/484 (31.40%), Postives = 242/484 (50.00%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            TK+FIN ++  S + +   + NPAT + I  V +  K ++++AV++   AF     W +     R + + KL +L++RD   +A   +   GK  L      ++G ++ + +     +   G ++P   + L    Y EP+GV   I P+NFP M+  W    +L CGNT++LKPSE  P   + +  L  EAGFPPG VN++ G    A   I  H  I  ++F G  + G+ I E  G  N KRV   +G KN  ++  DA  +  + Q     F  AGQ C A  + ++V E     ++   + RAK   V     P  + GP IS+  + R+   I+SG +EGAK+   G+ L + G+    F+ PT+ ++V   M   KEEIFGPV   ++  T+++AI+  NN+ YG   A+FT     A   +  +  G V VN    +    P   F G + S  G+    G+ G+  + ++KT+T
Sbjct:   38 TKIFINNEWHVSVSGKVFPVYNPATGEQICEVQEADKADVDKAVQAARLAFSLGSVWRRMDASERGKLLAKLADLVERDSVYLATIESLNSGKPFLPTLFVDLQGTIKTLRYFAGYADKIHGTSIPMDGEYLTFTRY-EPIGVCGQIIPWNFPLMMAAWKLAPALACGNTVVLKPSEETPLTCLYVAALVKEAGFPPGVVNILPGFGPTAGAAIASHMGIDKVAFTGSTEVGKLIQEAAGRSNLKRVTLEMGGKNPNIIFADADLDLAVEQAHQGVFFNAGQCCTA-GSRIYVEEPIYDEFVRRSVERAKRRIVGSPFDPTTEQGPQISREHQNRVLEYIQSGIQEGAKLECGGKALGLKGF----FIEPTVFSDVKDDMRIAKEEIFGPVQQIMKFKTVEEAIERANNSDYGLTAAVFTTDINKAMTVSTAMQAGTVWVNCFNALSTQCP---FGGYKMS--GNGRELGESGLKEYLEIKTIT 510          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Match: aldh1a2 (aldehyde dehydrogenase 1 family member A2 [Source:NCBI gene;Acc:100049450])

HSP 1 Score: 213.772 bits (543), Expect = 3.787e-62
Identity = 142/441 (32.20%), Postives = 223/441 (50.57%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRG-LQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHG-QHDAVNFICDHPKIRAISFVGGDKAGRHIYE-RGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGE--TRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVN 1430
            TK+FIN ++  S + +   + NPAT + I  V +  K ++++AV++   AF     W +     R + + KL +L++RD   +A   +   GK  L      ++G ++ + +     +   G ++P   + L    Y EP+GV   I P+NFP M+  W    +L CGNT++LKPSE  P   + +  L  EAGFPPG VN++ G    A   I  H  I  ++F G  + G+ I E  G  N KRV   +G KN  ++  DA  +  + Q     F  AGQ C A  + ++V E     ++   + RAK   V     P  + GP IS+  + R+   I+SG +EGAK+   G+ L + G+    F+ PT+ ++V   M   KEEIFGPV   ++  T+++AI+  NN+ YG   A+FT     A   +  +  G V VN
Sbjct:   38 TKIFINNEWHVSVSGKVFPVYNPATGEQICEVQEADKADVDKAVQAARLAFSLGSVWRRMDASERGKLLAKLADLVERDSVYLATIESLNSGKPFLPTLFVDLQGTIKTLRYFAGYADKIHGTSIPMDGEYLTFTRY-EPIGVCGQIIPWNFPLMMAAWKLAPALACGNTVVLKPSEETPLTCLYVAALVKEAGFPPGVVNILPGFGPTAGAAIASHMGIDKVAFTGSTEVGKLIQEAAGRSNLKRVTLEMGGKNPNIIFADADLDLAVEQAHQGVFFNAGQCCTA-GSRIYVEEPIYDEFVRRSVERAKRRIVGSPFDPTTEQGPQISREHQNRVLEYIQSGIQEGAKLECGGKALGLKGF----FIEPTVFSDVKDDMRIAKEEIFGPVQQIMKFKTVEEAIERANNSDYGLTAAVFTTDINKAMTVSTAMQAGTVWVN 472          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Match: SMESG000039266.1 (SMESG000039266.1)

HSP 1 Score: 1087.4 bits (2811), Expect = 0.000e+0
Identity = 521/523 (99.62%), Postives = 522/523 (99.81%), Query Frame = 3
Query:   51 MFYIASKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 1619
            MFYI SKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQ+IHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM
Sbjct:    1 MFYIVSKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQRIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVTQLWREEDAEHDSKGSATNFPTM 523          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Match: SMESG000016195.1 (SMESG000016195.1)

HSP 1 Score: 215.698 bits (548), Expect = 9.058e-63
Identity = 153/482 (31.74%), Postives = 244/482 (50.62%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNG-KRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            TK+FIN ++ DS + +     NPAT + I +V +  K ++++AV +  +AF+    W +    +R   M+KL +LI+RD   +AA  T + GK +  A   DV   ++  E+     +   G+ +P I  D    +  EP+GV   I P+NFP ++  W    +L  GNT++LK +E+ P     +  L  EAGFPPG VN++ G    A   +  HPK+  I+F G  + G+ I +  ++N  KR+   +G K+  ++  DA  ++ +     A F   GQ C A  + ++V E     ++  ++A AK+ KV        D GP I +    +I   I+SG  EGA++   G+ +   G+    F+ PTI T V  SM   KEEIFGPV+  ++    ++ I   NNN YG   +IFT     +   TQ +  G V VN    V      F G + S +G     G+ G++ +T++K VT
Sbjct:   41 TKIFINNEWHDSVSKKTFPTKNPATGQDICQVAEGGKEDIDKAVTAANKAFQLGSPWRKMDAADRGSLMYKLAQLIRRDADYLAALETLDSGKPVSIARAADVELTIKCYEYYAGWADKNHGKVIP-IRGDYIGFTRHEPVGVCGQIIPWNFPLLMQAWKLAPALSMGNTVVLKTAEQTPLTANYVASLAAEAGFPPGVVNIVPGMGPTAGAALVTHPKVDKIAFTGSTEVGQIISKHCNENNIKRLTLELGGKSPNIVFADADFDNAVKTSYFALFFNMGQCCCA-GSRIYVEEKIYDKFVEAMVAEAKS-KVVGNPFDNVDQGPQIDEEQMNKILGFIDSGKSEGAQMCTGGKQIGSKGF----FIEPTIFTKVEDSMRIAKEEIFGPVMQIMKFKDTNEVIARANNNEYGLAASIFTQDIDKSLHVTQSLRAGTVWVNC-YDVFDAAAPFGGYKSSGVGRE--LGEYGLSNYTEVKMVT 512          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Match: SMESG000016195.1 (SMESG000016195.1)

HSP 1 Score: 215.698 bits (548), Expect = 1.085e-62
Identity = 153/482 (31.74%), Postives = 244/482 (50.62%), Query Frame = 3
Query:  132 TKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE---SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTLLDAEG-DVMRGLQVVEHCCSITNLQLGETMPGIAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQH-DAVNFICDHPKIRAISFVGGDKAGRHIYERGSKNG-KRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGET--RNWIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            TK+FIN ++ DS + +     NPAT + I +V +  K ++++AV +  +AF+    W +    +R   M+KL +LI+RD   +AA  T + GK +  A   DV   ++  E+     +   G+ +P I  D    +  EP+GV   I P+NFP ++  W    +L  GNT++LK +E+ P     +  L  EAGFPPG VN++ G    A   +  HPK+  I+F G  + G+ I +  ++N  KR+   +G K+  ++  DA  ++ +     A F   GQ C A  + ++V E     ++  ++A AK+ KV        D GP I +    +I   I+SG  EGA++   G+ +   G+    F+ PTI T V  SM   KEEIFGPV+  ++    ++ I   NNN YG   +IFT     +   TQ +  G V VN    V      F G + S +G     G+ G++ +T++K VT
Sbjct:   37 TKIFINNEWHDSVSKKTFPTKNPATGQDICQVAEGGKEDIDKAVTAANKAFQLGSPWRKMDAADRGSLMYKLAQLIRRDADYLAALETLDSGKPVSIARAADVELTIKCYEYYAGWADKNHGKVIP-IRGDYIGFTRHEPVGVCGQIIPWNFPLLMQAWKLAPALSMGNTVVLKTAEQTPLTANYVASLAAEAGFPPGVVNIVPGMGPTAGAALVTHPKVDKIAFTGSTEVGQIISKHCNENNIKRLTLELGGKSPNIVFADADFDNAVKTSYFALFFNMGQCCCA-GSRIYVEEKIYDKFVEAMVAEAKS-KVVGNPFDNVDQGPQIDEEQMNKILGFIDSGKSEGAQMCTGGKQIGSKGF----FIEPTIFTKVEDSMRIAKEEIFGPVMQIMKFKDTNEVIARANNNEYGLAASIFTQDIDKSLHVTQSLRAGTVWVNC-YDVFDAAAPFGGYKSSGVGRE--LGEYGLSNYTEVKMVT 508          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Match: SMESG000005275.1 (SMESG000005275.1)

HSP 1 Score: 205.682 bits (522), Expect = 1.158e-56
Identity = 147/509 (28.88%), Postives = 252/509 (49.51%), Query Frame = 3
Query:   63 ASKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE--SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTL-LDAEGDVMRGLQVVEHCCSITNLQLGETMP-GIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHD-AVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRN--WIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            + K+L      ++ N ++  IP  ++FIN +FV+S++ +  D  NP+   VI RV   +K +++ AV++  EAFE   W + S  +R + ++KL +L++   +++A   + + G    L  +  +   ++   +     +   G T+P   A+    +S+  KEP+GV   ITP+N+P M+  W     L  GNT++LKP++  P A++   EL VEAGFP G +NV+ G    A   I +HP IR + F G    G+ I    +K N K+    +G K+  ++  D   +  +     + F   G+ C+A +  +FV    +  ++  ++   K +K+ + +    D GP   +    ++    + G KEGAK+V  G+     GY    F+ PTI T+V   M   +EE FGP++I  +   +D+ I   N   YG  + +FTN  + A +    I  G V +N      V  P   F G + S  G     G++ +N + + KTVT
Sbjct:  413 SQKKLHYDAVIINANKMTFPIPH-QIFINNEFVNSESGKEYDSKNPSDESVICRVSYSSKKDVDIAVKAAKEAFEFGEWGKMSARDRGRILYKLADLMEEHAEELATLESLDSGAVYTLALKTHIGMSIETFRYFAGWCDKIQGSTIPISNARPNKNLSFTKKEPIGVCGIITPWNYPLMMLAWKVAACLAAGNTVVLKPAQVTPLASLKFCELSVEAGFPSGVINVLPGSGAIAGTAISEHPDIRKLGFTGSTAIGQTIMSSCAKSNLKKCSLELGGKSPLIIFSDCDMDKAVKSACSSVFFNKGENCIA-AGRIFVDVRIHDVFLKLVVEDVKKMKIGDPLDRSVDHGPQNHEIHLLKLLEFCDIGVKEGAKLVFGGKRCDRKGY----FMEPTIFTSVTDDMYIAREESFGPIMIISKFYDIDEVIVRANKTEYGLASGVFTNDISKALRVADAIDAGTVFINTYNKTDVAAP---FGGFKQSGFGKD--LGQEALNEYLKTKTVT 910          
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Match: SMESG000005275.1 (SMESG000005275.1)

HSP 1 Score: 205.297 bits (521), Expect = 1.210e-56
Identity = 147/509 (28.88%), Postives = 252/509 (49.51%), Query Frame = 3
Query:   63 ASKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNPATNKVISRVPKCTKPEMERAVESCAEAFE--SWSQRSVLNRQQYMFKLQELIKRDLKKIAANITSEQGKTL-LDAEGDVMRGLQVVEHCCSITNLQLGETMP-GIAKDLDIVSY--KEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGATMMLMELCVEAGFPPGTVNVIHGQHD-AVNFICDHPKIRAISFVGGDKAGRHIYERGSK-NGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGAAGQRCMALSTAVFVGETRN--WIPDIIARAKNLKVSEGMQPGADLGPVISKHAKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSMECYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQKIHVGQVGVNV--PIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTVT 1553
            + K+L      ++ N ++  IP  ++FIN +FV+S++ +  D  NP+   VI RV   +K +++ AV++  EAFE   W + S  +R + ++KL +L++   +++A   + + G    L  +  +   ++   +     +   G T+P   A+    +S+  KEP+GV   ITP+N+P M+  W     L  GNT++LKP++  P A++   EL VEAGFP G +NV+ G    A   I +HP IR + F G    G+ I    +K N K+    +G K+  ++  D   +  +     + F   G+ C+A +  +FV    +  ++  ++   K +K+ + +    D GP   +    ++    + G KEGAK+V  G+     GY    F+ PTI T+V   M   +EE FGP++I  +   +D+ I   N   YG  + +FTN  + A +    I  G V +N      V  P   F G + S  G     G++ +N + + KTVT
Sbjct:  387 SQKKLHYDAVIINANKMTFPIPH-QIFINNEFVNSESGKEYDSKNPSDESVICRVSYSSKKDVDIAVKAAKEAFEFGEWGKMSARDRGRILYKLADLMEEHAEELATLESLDSGAVYTLALKTHIGMSIETFRYFAGWCDKIQGSTIPISNARPNKNLSFTKKEPIGVCGIITPWNYPLMMLAWKVAACLAAGNTVVLKPAQVTPLASLKFCELSVEAGFPSGVINVLPGSGAIAGTAISEHPDIRKLGFTGSTAIGQTIMSSCAKSNLKKCSLELGGKSPLIIFSDCDMDKAVKSACSSVFFNKGENCIA-AGRIFVDVRIHDVFLKLVVEDVKKMKIGDPLDRSVDHGPQNHEIHLLKLLEFCDIGVKEGAKLVFGGKRCDRKGY----FMEPTIFTSVTDDMYIAREESFGPIMIISKFYDIDEVIVRANKTEYGLASGVFTNDISKALRVADAIDAGTVFINTYNKTDVAAP---FGGFKQSGFGKD--LGQEALNEYLKTKTVT 884          
The following BLAST results are available for this feature:
BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ALDH6A10.000e+068.34aldehyde dehydrogenase 6 family member A1 [Source:... [more]
ALDH6A10.000e+067.33aldehyde dehydrogenase 6 family member A1 [Source:... [more]
ALDH6A15.125e-13271.43aldehyde dehydrogenase 6 family member A1 [Source:... [more]
ALDH1A12.590e-6832.72aldehyde dehydrogenase 1 family member A1 [Source:... [more]
ALDH1A34.415e-6832.80aldehyde dehydrogenase 1 family member A3 [Source:... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alh-80.000e+065.45Probable methylmalonate-semialdehyde dehydrogenase... [more]
alh-80.000e+065.45Probable methylmalonate-semialdehyde dehydrogenase... [more]
alh-80.000e+065.45Probable methylmalonate-semialdehyde dehydrogenase... [more]
alh-21.545e-6230.58ALdehyde deHydrogenase [Source:UniProtKB/TrEMBL;A... [more]
alh-11.025e-5529.35ALdehyde deHydrogenase [Source:UniProtKB/TrEMBL;A... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG178960.000e+067.45gene:FBgn0023537 transcript:FBtr0070092[more]
CG178960.000e+067.45gene:FBgn0023537 transcript:FBtr0070093[more]
CG310758.754e-6432.99gene:FBgn0051075 transcript:FBtr0085080[more]
CG310754.050e-6232.36gene:FBgn0051075 transcript:FBtr0307506[more]
Ssadh8.022e-6027.18gene:FBgn0039349 transcript:FBtr0100569[more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aldh6a10.000e+066.54aldehyde dehydrogenase 6 family, member A1 [Source... [more]
aldh9a1a.11.670e-6630.30aldehyde dehydrogenase 9 family, member A1a, tande... [more]
aldh9a1a.11.670e-6630.30aldehyde dehydrogenase 9 family, member A1a, tande... [more]
aldh9a1a.11.670e-6630.30aldehyde dehydrogenase 9 family, member A1a, tande... [more]
aldh9a1a.11.670e-6630.30aldehyde dehydrogenase 9 family, member A1a, tande... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tlr30.000e+068.33toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-4... [more]
tlr30.000e+067.79toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-4... [more]
tlr30.000e+068.47toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-4... [more]
tlr30.000e+068.47toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-4... [more]
tlr30.000e+068.47toll like receptor 3 [Source:Xenbase;Acc:XB-GENE-4... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Aldh6a10.000e+068.94aldehyde dehydrogenase family 6, subfamily A1 [Sou... [more]
Aldh1a71.355e-6833.13aldehyde dehydrogenase family 1, subfamily A7 [Sou... [more]
Aldh1a11.473e-6832.72aldehyde dehydrogenase family 1, subfamily A1 [Sou... [more]
Aldh1a11.473e-6832.72aldehyde dehydrogenase family 1, subfamily A1 [Sou... [more]
Aldh1a32.084e-6632.80aldehyde dehydrogenase family 1, subfamily A3 [Sou... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q17M80|MMSA_AEDAE0.000e+071.37Probable methylmalonate-semialdehyde dehydrogenase... [more]
sp|Q7QC84|MMSA_ANOGA0.000e+070.39Probable methylmalonate-semialdehyde dehydrogenase... [more]
sp|Q9EQ20|MMSA_MOUSE0.000e+068.94Methylmalonate-semialdehyde dehydrogenase [acylati... [more]
sp|Q02253|MMSA_RAT0.000e+068.34Methylmalonate-semialdehyde dehydrogenase [acylati... [more]
sp|Q02252|MMSA_HUMAN0.000e+068.34Methylmalonate-semialdehyde dehydrogenase [acylati... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V5IF880.000e+073.62Putative methylmalonate semialdehyde dehydrogenase... [more]
A0A4D5RHE70.000e+073.43Putative methylmalonate-semialdehyde dehydrogenase... [more]
A0A131YMU90.000e+073.06Methylmalonate-semialdehyde dehydrogenase OS=Rhipi... [more]
A0A224YTE80.000e+072.87Methylmalonate-semialdehyde dehydrogenase OS=Rhipi... [more]
A0A131Y2Q80.000e+073.43Putative methylmalonate semialdehyde dehydrogenase... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aldh1a24.039e-6530.60aldehyde dehydrogenase 1 family member A2 [Source:... [more]
aldh1a23.376e-6331.31aldehyde dehydrogenase 1 family member A2 [Source:... [more]
aldh1a32.013e-6131.64aldehyde dehydrogenase 1 family member A3 [Source:... [more]
aldh1a32.000e-6031.03aldehyde dehydrogenase 1 family member A3 [Source:... [more]
aldh9a1b2.589e-6027.49aldehyde dehydrogenase 9 family member A1 [Source:... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aldh6a10.000e+066.87aldehyde dehydrogenase 6 family, member A1 [Source... [more]
ENSPMAT00000004609.11.985e-5627.86pep scaffold:Pmarinus_7.0:GL479321:13801:23388:1 g... [more]
aldh5a14.036e-4828.76aldehyde dehydrogenase 5 family, member A1 (succin... [more]
aldh1l26.778e-4726.91aldehyde dehydrogenase 1 family, member L2 [Source... [more]
aldh8a11.018e-4028.72aldehyde dehydrogenase 8 family, member A1 [Source... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
ALD43.536e-6128.54Mitochondrial aldehyde dehydrogenase; required for... [more]
ALD51.169e-6029.29Mitochondrial aldehyde dehydrogenase; involved in ... [more]
ALD24.597e-5830.16Cytoplasmic aldehyde dehydrogenase; involved in et... [more]
ALD32.875e-5530.36Cytoplasmic aldehyde dehydrogenase; involved in be... [more]
ALD61.450e-5227.83Cytosolic aldehyde dehydrogenase; activated by Mg2... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO495720.000e+068.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO455385.995e-6431.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO361831.371e-5632.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO476531.813e-5529.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO343622.926e-5527.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aldh6a10.000e+069.12aldehyde dehydrogenase 6 family member A1 [Source:... [more]
ENSORLT00000045168.10.000e+068.33methylmalonate-semialdehyde dehydrogenase [acylati... [more]
ENSORLT00000011823.29.411e-15867.21methylmalonate-semialdehyde dehydrogenase [acylati... [more]
aldh1a22.931e-6331.40aldehyde dehydrogenase 1 family member A2 [Source:... [more]
aldh1a23.787e-6232.20aldehyde dehydrogenase 1 family member A2 [Source:... [more]
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BLAST of Methylmalonate-semialdehyde dehydrogenase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039266.10.000e+099.62SMESG000039266.1[more]
SMESG000016195.19.058e-6331.74SMESG000016195.1[more]
SMESG000016195.11.085e-6231.74SMESG000016195.1[more]
SMESG000005275.11.158e-5628.88SMESG000005275.1[more]
SMESG000005275.11.210e-5628.88SMESG000005275.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032682 ID=SMED30032682|Name=Methylmalonate-semialdehyde dehydrogenase|organism=Schmidtea mediterranea sexual|type=transcript|length=1758bp
AATTCCTTAAAACACTTTAAACAAGCAGTAATTAAATTTAAATATTGAAA
ATGTTTTATATTGCTTCAAAAAGATTAACCTTACGAGTGCATGCCTTGTC
CAGAAATTATCTATCCACTGCTATTCCAAAAACTAAAATGTTCATCAATG
GGGATTTCGTTGATTCTCAAACCACAGAATGGATTGATTTACCGAATCCA
GCAACAAATAAAGTCATCAGTCGTGTACCTAAGTGTACAAAACCTGAAAT
GGAAAGAGCTGTAGAATCATGTGCTGAGGCTTTCGAAAGTTGGTCACAGC
GATCAGTGCTGAATCGACAGCAATATATGTTTAAATTGCAGGAATTAATA
AAGCGTGATTTAAAAAAAATTGCAGCCAATATTACGAGCGAACAGGGTAA
AACACTCCTAGATGCTGAAGGGGATGTCATGCGGGGTTTGCAAGTAGTAG
AGCATTGCTGTAGCATTACTAATCTTCAACTTGGAGAAACAATGCCTGGA
ATTGCTAAAGATTTAGACATTGTTAGTTACAAAGAACCTCTTGGAGTTAC
TGCTGGAATAACACCTTTTAATTTTCCCGCGATGATTCCATTATGGATGT
TTCCATTGTCTCTGGTGTGTGGAAATACCATGATTCTAAAACCAAGTGAA
AGAGATCCAGGTGCTACTATGATGCTGATGGAATTATGTGTGGAAGCTGG
TTTTCCACCTGGAACTGTTAATGTCATTCATGGACAACATGATGCTGTTA
ATTTCATATGTGACCATCCTAAAATTAGGGCGATCTCGTTTGTAGGAGGT
GATAAGGCTGGAAGGCATATCTATGAAAGAGGTTCAAAAAATGGCAAGAG
AGTTCAAGCTAATATAGGAGCAAAGAATCACGGTGTATTAATGCCAGATG
CAAGAAAGGAAAGTACAATTAATCAACTAGTTGGTGCAGCATTTGGAGCT
GCCGGTCAACGCTGTATGGCATTGAGTACAGCCGTATTTGTCGGTGAAAC
TCGTAATTGGATTCCAGACATCATAGCGCGTGCAAAAAATCTAAAAGTAT
CAGAAGGCATGCAACCAGGAGCTGATCTTGGCCCAGTTATTAGCAAGCAC
GCTAAAGAAAGAATTCATTCCCTTATTGAATCTGGTACAAAGGAAGGGGC
TAAAATCGTGTTAGATGGCAGAAATTTAAAAGTATCTGGATATGAAGATG
GAAATTTTGTCGGACCTACAATTCTCACAAATGTCAATCCTAGCATGGAG
TGCTATAAAGAAGAAATATTTGGTCCGGTATTGATTTGTTTAGAAGTTGA
TACACTGGATGATGCTATAAAATTGATTAATAACAATCCATATGGTAATG
GCACAGCAATATTCACAAATAGTGGTGCTACGGCAAGGAAATTCACTCAG
AAAATTCATGTTGGCCAAGTTGGTGTCAATGTTCCTATTCCAGTTCCGCT
TCCAATGTTCAGTTTTACTGGATCTCGTGGTTCATTTCTTGGAGATACCA
ATTTCTATGGGAAACAGGGTGTCAATTTCTTTACACAACTGAAAACCGTG
ACGCAACTGTGGCGTGAGGAAGACGCTGAGCATGATAGTAAAGGCTCGGC
AACTAATTTTCCAACTATGTAAAATGAGATCAGAGCTAAAGTTTTTAACA
TGCGCTAATTATCATGCTAAAAAATTGTCTAGTTGTGTTTAGTAGATGAT
CTTGTTTTTGGAAAATATTTTTCAATTTTAATACAAAATAACTGTGTTTG
CAAAAAAA
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protein sequence of SMED30032682-orf-1

>SMED30032682-orf-1 ID=SMED30032682-orf-1|Name=SMED30032682-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=524bp
MFYIASKRLTLRVHALSRNYLSTAIPKTKMFINGDFVDSQTTEWIDLPNP
ATNKVISRVPKCTKPEMERAVESCAEAFESWSQRSVLNRQQYMFKLQELI
KRDLKKIAANITSEQGKTLLDAEGDVMRGLQVVEHCCSITNLQLGETMPG
IAKDLDIVSYKEPLGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSE
RDPGATMMLMELCVEAGFPPGTVNVIHGQHDAVNFICDHPKIRAISFVGG
DKAGRHIYERGSKNGKRVQANIGAKNHGVLMPDARKESTINQLVGAAFGA
AGQRCMALSTAVFVGETRNWIPDIIARAKNLKVSEGMQPGADLGPVISKH
AKERIHSLIESGTKEGAKIVLDGRNLKVSGYEDGNFVGPTILTNVNPSME
CYKEEIFGPVLICLEVDTLDDAIKLINNNPYGNGTAIFTNSGATARKFTQ
KIHVGQVGVNVPIPVPLPMFSFTGSRGSFLGDTNFYGKQGVNFFTQLKTV
TQLWREEDAEHDSKGSATNFPTM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000419prepharyngeal region
PLANA:0000421tail
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR010061MeMal-semiAld_DH
IPR016162Ald_DH_N
IPR015590Aldehyde_DH_dom
IPR016160Ald_DH_CS_CYS
IPR016161Ald_DH/histidinol_DH
IPR016163Ald_DH_C
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
GO:0016620oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0004491methylmalonate-semialdehyde dehydrogenase (acylating) activity
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
GO:0055114oxidation-reduction process
GO:0006574valine catabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010061Methylmalonate-semialdehyde dehydrogenaseTIGRFAMTIGR01722TIGR01722coord: 29..504
e-value: 7.5E-230
score: 761.5
IPR010061Methylmalonate-semialdehyde dehydrogenasePANTHERPTHR43866FAMILY NOT NAMEDcoord: 16..516
IPR010061Methylmalonate-semialdehyde dehydrogenaseCDDcd07085ALDH_F6_MMSDHcoord: 28..504
e-value: 0.0
score: 895.336
IPR016162Aldehyde dehydrogenase, N-terminalGENE3DG3DSA:3.40.605.10coord: 18..273
e-value: 1.1E-76
score: 259.6
IPR015590Aldehyde dehydrogenase domainPFAMPF00171Aldedhcoord: 36..500
e-value: 1.0E-131
score: 439.7
IPR016163Aldehyde dehydrogenase, C-terminalGENE3DG3DSA:3.40.309.10coord: 275..506
e-value: 2.7E-60
score: 205.7
NoneNo IPR availablePANTHERPTHR43866:SF3METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIALcoord: 16..516
IPR016160Aldehyde dehydrogenase, cysteine active sitePROSITEPS00070ALDEHYDE_DEHYDR_CYScoord: 298..309
IPR016161Aldehyde/histidinol dehydrogenaseSUPERFAMILYSSF53720ALDH-likecoord: 19..502