Spermatogenesis associated 7

Overview
NameSpermatogenesis associated 7
Smed IDSMED30032666
Length (bp)1774
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Spermatogenesis associated 7 (SMED30032666) t-SNE clustered cells

Violin plots show distribution of expression levels for Spermatogenesis associated 7 (SMED30032666) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Spermatogenesis associated 7 (SMED30032666) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Spermatogenesis associated 7 (SMED30032666) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032666

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30032666h1SMcG0001016 SmedASXL_010852SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30032666h1SMcG0001016 dd_Smed_v4_8977_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30032666h1SMcG0001016 dd_Smed_v4_8977_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30032666h1SMcG0001016 dd_Smed_v4_8977_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032666h1SMcG0001016 dd_Smed_v4_8977_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30032666h1SMcG0001016 dd_Smed_v6_8977_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30032666h1SMcG0001016 dd_Smed_v4_8977_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Spermatogenesis associated 7 vs. Ensembl Xenopus
Match: ENSXETT00000020408.1 (spermatogenesis-associated protein 7 homolog [Source:NCBI gene;Acc:100493599])

HSP 1 Score: 55.8398 bits (133), Expect = 1.032e-7
Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = 3 
Query:  591 LP-ARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQGKQG---SFKSDGKMY 842
            LP AR+  +  +EE+I+YL F  DVTND+LI    ++R I  +F  H++R  +++  +      + ++ ELQG      S  +DG  Y
Sbjct:  235 LPGARRSQHLEQEEEIKYLRFLQDVTNDILIRGCGSERTIENVFQEHLQRKEHDIDEENKKHLMEELKAELQGSPKLDFSISNDGTQY 322
[Close]
BLAST of Spermatogenesis associated 7 vs. Ensembl Xenopus
Match: ENSXETT00000029977.1 (spermatogenesis associated 7 [Source:NCBI gene;Acc:100498037])

HSP 1 Score: 51.2174 bits (121), Expect = 3.872e-6
Identity = 25/86 (29.07%), Postives = 50/86 (58.14%), Query Frame = 3 
Query:   57 IIVASIVTVY*FKMSLKIEGHLTSKSSPLAPVSCK-LTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIK 311
            +++    ++  + +S   +GHL++KS+  +  S   L++Q+ ++ H+  HY  I   K++ID   PKS  K++   DQ R+E++ K
Sbjct:    5 VLIGRAPSIPRYGISSPFKGHLSTKSNAFSTGSSSWLSEQYRIRDHMVVHYNKILAAKAAIDCSVPKSMQKSIKYSDQQRREKMKK 90
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BLAST of Spermatogenesis associated 7 vs. Ensembl Mouse
Match: Spata7 (spermatogenesis associated 7 [Source:MGI Symbol;Acc:MGI:2144877])

HSP 1 Score: 56.225 bits (134), Expect = 6.226e-8
Identity = 54/235 (22.98%), Postives = 114/235 (48.51%), Query Frame = 3 
Query:  114 GHLTSKSSPLA--PVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKS-ARDHVRERNSPNIFQNKTFRSEAEDDTIQTNNRMSYEDLKPIGHRI---YKKDVKSKIAQLRCYNPPKIKSAVKTNSSAALISNTPAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNEL 800
            GHL++KS+       S +L+   +V+ H+  HY  I + K+++D   P S   ++   DQ R+E+L K  AR              K F+       +QTN++M+ +       ++    +   + KIA L    P + ++    +    ++  +  +   K  +  +      ++EE++ YL+F  +VT+++L   +F++R + ++F+ HIK+N ++L+  +M      ++ +L
Sbjct:   24 GHLSTKSNAFCTDSSSLRLSTLQLVKNHMAIHYNKILSAKAAVDCSIPVSVNTSIKYADQQRREKLRKELAR------------CEKEFK--LSKSAMQTNSKMNSKFFVNSLQKVPNYTRNGAEDKIAPL----PSQGQNLAWDSIQDGILQQSSERASCKLSTEFSPDSKIYSDEEELLYLSFMENVTDEILKLGLFSNRFLERLFERHIKKNKHHLEEGKMRYLLHGLKVDL 240
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BLAST of Spermatogenesis associated 7 vs. TrEMBL
Match: A0A2I0MAR1 (Spermatogenesis-associated protein 7 homolog (Fragment) OS=Columba livia OX=8932 GN=A306_00008693 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.187e-10
Identity = 32/76 (42.11%), Postives = 54/76 (71.05%), Query Frame = 3 
Query:   90 FKMSLKIEGHLTSKSSPLAP-VSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKS 314
            + M     GH++ KSSP +P  SCKL+ Q+++Q H+ AHYKN+ + K+++D+  PKS + +V  KDQ +++RLI++
Sbjct:    5 YSMKGPFRGHMSLKSSPFSPGPSCKLSNQYLIQGHMAAHYKNLMSAKAAVDSSAPKSLHTSVKYKDQQKRDRLIQA 80
[Close]
BLAST of Spermatogenesis associated 7 vs. TrEMBL
Match: A0A3Q0H4N4 (spermatogenesis-associated protein 7 homolog isoform X1 OS=Alligator sinensis OX=38654 GN=LOC102385443 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.232e-10
Identity = 34/95 (35.79%), Postives = 59/95 (62.11%), Query Frame = 3 
Query:   54 LIIVASIVTVY*FKMSLKIEGHLTSKSSPLAP-VSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRE 335
            ++I + +  V  + M     GH+  KSSP +P  SC L+ Q+I+Q H+  HYK + + K+++D+  PKS + ++  +DQ RK+RL+K+   + RE
Sbjct:   24 ILIRSQLAMVPKYSMMGPFRGHMCIKSSPFSPGSSCNLSSQYIIQDHMATHYKKLLSAKAAVDSSVPKSLHTSIQYRDQQRKDRLVKAVEKYKRE 118
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BLAST of Spermatogenesis associated 7 vs. TrEMBL
Match: A0A498NTZ5 (Spermatogenesis-associated 7-like protein OS=Labeo rohita OX=84645 GN=ROHU_014504 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.975e-10
Identity = 49/154 (31.82%), Postives = 83/154 (53.90%), Query Frame = 3 
Query:  354 FQNKTFRSEAEDDTIQTNNRMSYEDLKPIGHRIYKKDVKSKIAQLRCYNPPKIKSAVKTNSSAALISNT----PAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQ 803
            FQ+ T ++ + D  ++  +R + E  KP   R  K D KS + Q R Y PP+ K+  + +SS  +   T     + E  + P +  RK     EEE++ YL F  DVTN++L   +++DR + ++F+ HI  N + L  ++M    DN++ +LQ
Sbjct:  161 FQDPTQKTYSGDVLLKHAHRFTQE--KPFTPRTLKSDHKSTLLQYRYYTPPRRKAEEERSSSKVVRQETYHGRISPEGMRSPIM--RK--VSAEEEELMYLEFITDVTNEILTQGLYSDRVLKRVFERHIDMNKHRLDENKMRHLLDNLQEDLQ 308
[Close]
BLAST of Spermatogenesis associated 7 vs. TrEMBL
Match: A0A1U7SCU9 (spermatogenesis-associated protein 7 homolog isoform X2 OS=Alligator sinensis OX=38654 GN=LOC102385443 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.273e-10
Identity = 32/83 (38.55%), Postives = 53/83 (63.86%), Query Frame = 3 
Query:   90 FKMSLKIEGHLTSKSSPLAP-VSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRE 335
            + M     GH+  KSSP +P  SC L+ Q+I+Q H+  HYK + + K+++D+  PKS + ++  +DQ RK+RL+K+   + RE
Sbjct:    5 YSMMGPFRGHMCIKSSPFSPGSSCNLSSQYIIQDHMATHYKKLLSAKAAVDSSVPKSLHTSIQYRDQQRKDRLVKAVEKYKRE 87
[Close]
BLAST of Spermatogenesis associated 7 vs. TrEMBL
Match: A0A151P3G5 (Spermatogenesis-associated 7-like protein OS=Alligator mississippiensis OX=8496 GN=Y1Q_0013619 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.057e-9
Identity = 32/83 (38.55%), Postives = 53/83 (63.86%), Query Frame = 3 
Query:   90 FKMSLKIEGHLTSKSSPLAP-VSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRE 335
            + M     GH+  KSSP +P  SC L+ Q+I+Q H+  HYK + + K+++D+  PKS + ++  +DQ RK+RL+K+   + RE
Sbjct:   47 YSMMGPFRGHMCIKSSPFSPGSSCNLSSQYIIQDHMATHYKKLLSAKAAVDSSVPKSLHTSIKYRDQQRKDRLVKAVEKYKRE 129
[Close]
BLAST of Spermatogenesis associated 7 vs. Ensembl Cavefish
Match: spata7 (spermatogenesis associated 7 [Source:NCBI gene;Acc:103029867])

HSP 1 Score: 56.225 bits (134), Expect = 5.990e-8
Identity = 23/60 (38.33%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  624 EEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQ 803
            E++++YL F  DVT+D+L   +++DR +  +F+ HI  N + L  D+M    D++RN+LQ
Sbjct:  323 EQEVKYLEFITDVTDDILARGIYSDRVLELVFERHIDMNRHRLDKDKMRHLLDDLRNDLQ 382
[Close]
BLAST of Spermatogenesis associated 7 vs. Ensembl Nematostella
Match: EDO48533 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ78])

HSP 1 Score: 50.8322 bits (120), Expect = 1.867e-8
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 3 
Query:  114 GHLTSKSSPLAPVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNV 272
            GHLT KSS L+P +     Q +V  H+N+HY+ IS+ K+S+D   PKS  K++
Sbjct:   10 GHLTLKSSVLSPTAISYMGQAMVLNHMNSHYRRISSAKASVDTSAPKSMKKHI 62
[Close]
BLAST of Spermatogenesis associated 7 vs. Planmine SMEST
Match: SMESG000056890.1 (SMESG000056890.1)

HSP 1 Score: 845.884 bits (2184), Expect = 0.000e+0
Identity = 408/412 (99.03%), Postives = 410/412 (99.51%), Query Frame = 3 
Query:   96 MSLKIEGHLTSKSSPLAPVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRERNSPNIFQNKTFRSEAEDDTIQTNNRMSYEDLKPIGHRIYKKDVKSKIAQLRCYNPPKIKSAVKTNSSAALISNTPAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQGKQGSFKSDGKMYAENNITKNFAANILKTDANERNSNDIVQDASQNLNYENVTTAKQYYRSDEYKQENDNFSYNLHDPKKLDNDHDGNKLSENHLETKHYFKDYSTGDHSFSEGLRKPSDENEDSKYLLLTQKEQNNIENEYISPKSKPNEFSTAPSALQWSLTSNEDLHDLHSKF 1331
            MSLKIEGHLTSKSSPLAPVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRERNSPNIFQNKTFRSEAEDDTIQTNNRMSYEDLKPIGHRIYKKDVKSKIAQLRCYNPPKIKSAVKTNSSAALISNTPAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQGKQGSFKSDGKM+AENNITK FAANILKTDANERNSN+IVQDASQNLNYENVTTAKQYYRSDEYKQENDNFSYNLHDPKKLDNDHDGNKLSENHLETKHYFKDYSTGDHSFSEGLRKPSDENEDSKYLLLTQKEQNNIENEYISPKSKPNEFSTAPSALQWSLTSNEDLHDLHSK 
Sbjct:    1 MSLKIEGHLTSKSSPLAPVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNKCPKSWYKNVTVKDQLRKERLIKSARDHVRERNSPNIFQNKTFRSEAEDDTIQTNNRMSYEDLKPIGHRIYKKDVKSKIAQLRCYNPPKIKSAVKTNSSAALISNTPAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFTDRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQGKQGSFKSDGKMFAENNITKIFAANILKTDANERNSNEIVQDASQNLNYENVTTAKQYYRSDEYKQENDNFSYNLHDPKKLDNDHDGNKLSENHLETKHYFKDYSTGDHSFSEGLRKPSDENEDSKYLLLTQKEQNNIENEYISPKSKPNEFSTAPSALQWSLTSNEDLHDLHSKL 412
The following BLAST results are available for this feature:
BLAST of Spermatogenesis associated 7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000020408.11.032e-734.09spermatogenesis-associated protein 7 homolog [Sour... [more]
ENSXETT00000029977.13.872e-629.07spermatogenesis associated 7 [Source:NCBI gene;Acc... [more]
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BLAST of Spermatogenesis associated 7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Spata76.226e-822.98spermatogenesis associated 7 [Source:MGI Symbol;Ac... [more]
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BLAST of Spermatogenesis associated 7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2I0MAR11.187e-1042.11Spermatogenesis-associated protein 7 homolog (Frag... [more]
A0A3Q0H4N41.232e-1035.79spermatogenesis-associated protein 7 homolog isofo... [more]
A0A498NTZ53.975e-1031.82Spermatogenesis-associated 7-like protein OS=Labeo... [more]
A0A1U7SCU95.273e-1038.55spermatogenesis-associated protein 7 homolog isofo... [more]
A0A151P3G51.057e-938.55Spermatogenesis-associated 7-like protein OS=Allig... [more]
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BLAST of Spermatogenesis associated 7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spata75.990e-838.33spermatogenesis associated 7 [Source:NCBI gene;Acc... [more]
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BLAST of Spermatogenesis associated 7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO485331.867e-845.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Spermatogenesis associated 7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000056890.10.000e+099.03SMESG000056890.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032666 ID=SMED30032666|Name=Spermatogenesis associated 7|organism=Schmidtea mediterranea sexual|type=transcript|length=1774bp
AAATTGATAGCATTCTCCCGATGTAAACATTTATTTGTAAATAGTCATTT
GTTCTTATAATTGTCGCGTCTATAGTTACAGTGTATTAATTTAAAATGTC
ATTAAAAATTGAAGGTCATCTAACTTCAAAAAGTTCTCCTTTAGCACCAG
TTTCTTGTAAACTAACTAAACAATTTATTGTTCAACAACATCTTAATGCA
CATTATAAAAATATTTCTAATGTAAAATCTTCTATTGATAATAAATGCCC
AAAGTCGTGGTATAAAAATGTTACCGTCAAAGACCAGTTGAGAAAAGAGC
GACTAATTAAATCAGCAAGAGATCATGTAAGAGAAAGAAATTCGCCAAAT
ATATTTCAAAATAAAACTTTTCGTAGTGAGGCTGAAGATGATACCATTCA
AACTAATAACAGAATGAGTTATGAAGATCTCAAACCAATTGGACATCGAA
TATACAAAAAAGACGTCAAGTCAAAAATAGCACAATTAAGATGCTACAAT
CCCCCTAAAATTAAAAGTGCTGTTAAAACAAATTCTTCTGCGGCTTTGAT
ATCAAATACACCAGCACAAGAAGATTTCAAAGAACCTTCTCTACCAGCTA
GAAAAGATTTCTACAGAAATGAAGAAGAGGATATCAGATATTTGAATTTC
TGTAATGACGTTACGAATGACATGTTGATTAATAATGTCTTTACGGATCG
GGGGATTTATCAAATCTTTGACTACCACATTAAAAGAAATGTTTATAATC
TGAAAATGGACCAAATGCTTATAGCTTATGATAATATTCGAAATGAACTT
CAAGGCAAACAAGGCTCTTTTAAGTCAGACGGGAAAATGTATGCAGAAAA
CAATATTACTAAAAATTTCGCGGCAAATATTCTAAAAACTGACGCAAACG
AAAGAAATTCAAATGATATCGTCCAGGATGCATCACAAAACTTGAATTAT
GAAAACGTTACTACAGCGAAACAGTATTACCGAAGCGATGAATATAAACA
AGAAAATGATAATTTCAGTTACAATTTACACGATCCAAAGAAACTCGATA
ATGATCACGATGGAAATAAATTGAGCGAAAACCACTTGGAAACAAAACAT
TATTTCAAAGACTATTCCACTGGAGATCATTCATTTTCCGAAGGTTTAAG
AAAGCCCTCCGATGAGAATGAAGATAGCAAATATCTGTTGTTAACGCAAA
AAGAACAAAACAATATCGAAAATGAATACATCAGTCCAAAAAGTAAACCG
AATGAATTTTCCACAGCGCCATCGGCATTGCAGTGGTCATTGACATCGAA
TGAGGATTTACACGATTTACACTCGAAATTCGCTACCATGAAACCGACAC
CCGGTGAGCCGAGTATCACTGAGATCTGTGAAAAAATCGATAATGTCCAG
ATTGCAGAATCGGCCTCGAATTCTATCGAACGTGATCAGCGAAAAGAATC
GGACAAAAACTCTCCGGAAATTGAAAACAGCAATTTCAGCAAGAACGTTC
ATTTTGCCAATCAGAATGAAATCTATTCCGCTGCCGACAATGACGACCAT
TCACTGACAGATGTGACATCGATGCGATTGGAAGATGGCGATGAGAGCGA
TGATAACAACGATTCGACTTGTGTTGAATCGAGTTTGGACTTGAATAATA
CATTCAAAAGTGATGGAGTTAAAGACAACTCAAAACTGATCGGATATGAA
GATGATTTTGATGATTAATTATTATTATTATTATTTGTAATTTAAATAAA
CATTGAAAATACTTTAAAAAAAAA
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protein sequence of SMED30032666-orf-1

>SMED30032666-orf-1 ID=SMED30032666-orf-1|Name=SMED30032666-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=541bp
MSLKIEGHLTSKSSPLAPVSCKLTKQFIVQQHLNAHYKNISNVKSSIDNK
CPKSWYKNVTVKDQLRKERLIKSARDHVRERNSPNIFQNKTFRSEAEDDT
IQTNNRMSYEDLKPIGHRIYKKDVKSKIAQLRCYNPPKIKSAVKTNSSAA
LISNTPAQEDFKEPSLPARKDFYRNEEEDIRYLNFCNDVTNDMLINNVFT
DRGIYQIFDYHIKRNVYNLKMDQMLIAYDNIRNELQGKQGSFKSDGKMYA
ENNITKNFAANILKTDANERNSNDIVQDASQNLNYENVTTAKQYYRSDEY
KQENDNFSYNLHDPKKLDNDHDGNKLSENHLETKHYFKDYSTGDHSFSEG
LRKPSDENEDSKYLLLTQKEQNNIENEYISPKSKPNEFSTAPSALQWSLT
SNEDLHDLHSKFATMKPTPGEPSITEICEKIDNVQIAESASNSIERDQRK
ESDKNSPEIENSNFSKNVHFANQNEIYSAADNDDHSLTDVTSMRLEDGDE
SDDNNDSTCVESSLDLNNTFKSDGVKDNSKLIGYEDDFDD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR029357SPATA7