Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2

Overview


Name	Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2
Smed ID	SMED30032663
Length (bp)	1794

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 (SMED30032663) t-SNE clustered cells

Violin plots show distribution of expression levels for Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 (SMED30032663) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 (SMED30032663) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Anatomical Expression

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
tail region	SMED30032663	SmedASXL_012104	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
X2 cell	SMED30032663	SmedASXL_012104	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
Smed sexual biotype	SMED30032663	Contig52385	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032663	Contig52385	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032663	Contig40948	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032663	Contig40948	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032663	Contig54526	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032663	Contig54526	newmark_ests	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30032663	dd_Smed_v6_7_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Match: zgc:158463 (zgc:158463 [Source:ZFIN;Acc:ZDB-GENE-070410-9])

HSP 1 Score: 132.88 bits (333), Expect = 4.069e-36
Identity = 75/125 (60.00%), Postives = 87/125 (69.60%), Query Frame = 2

Query: 1373 DFLEE*IAFS*MKLSNNRSVMPLDVRGRTRATMAVTASFIPS*KWPGNLLNHCRVRNSGLKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNGNISIIG--RRQSNLII 1741
            +FLE  +AFS  + SNNRSVMPLDVRG TRATM  +    P+ +  GN LN  R  + GLKLF +NEEF VSA H LALI SLPFVHTARRYYRL+GLVRS D   R  +    G  RR+SNLI+
Sbjct:   14 NFLEGQVAFSHARWSNNRSVMPLDVRGCTRATMGGSTCAYPAPRGAGNPLNPARDWDWGLKLFPINEEFPVSAGHKLALIKSLPFVHTARRYYRLDGLVRSSDRPRRGSSPGPGGAPRRRSNLIL 138

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Match: AL935186.10 (pep chromosome:GRCz11:4:77561226:77561679:-1 gene:ENSDARG00000115978.1 transcript:ENSDART00000180809.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL935186.10)

HSP 1 Score: 118.627 bits (296), Expect = 6.844e-31
Identity = 64/97 (65.98%), Postives = 70/97 (72.16%), Query Frame = 2

Query: 1391 IAFS*MKLSNNRSVMPLDVRGRTRATMAVTASFIPS*KWPGNLLNHCRVRNSGLKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLD 1681
            +AFS  +LSNNRSVMPLDVRG TRATM  +    P+    GN LN  R  + GLKLF  NEEF VSA H LALI SLPFVHTARRYYRL+G VRS D
Sbjct:   13 VAFSHARLSNNRSVMPLDVRGCTRATMGGSTCAYPAPTGAGNPLNPARDGDRGLKLFPENEEFPVSAGHQLALIKSLPFVHTARRYYRLSGSVRSSD 109

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Match: AL935186.6 (pep chromosome:GRCz11:CHR_ALT_CTG4_1_15:77561226:77561679:-1 gene:ENSDARG00000111118.1 transcript:ENSDART00000189523.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL935186.6)

HSP 1 Score: 118.627 bits (296), Expect = 6.844e-31
Identity = 64/97 (65.98%), Postives = 70/97 (72.16%), Query Frame = 2

Query: 1391 IAFS*MKLSNNRSVMPLDVRGRTRATMAVTASFIPS*KWPGNLLNHCRVRNSGLKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLD 1681
            +AFS  +LSNNRSVMPLDVRG TRATM  +    P+    GN LN  R  + GLKLF  NEEF VSA H LALI SLPFVHTARRYYRL+G VRS D
Sbjct:   13 VAFSHARLSNNRSVMPLDVRGCTRATMGGSTCAYPAPTGAGNPLNPARDGDRGLKLFPENEEFPVSAGHQLALIKSLPFVHTARRYYRLSGSVRSSD 109

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Match: BX548011.1 (pep chromosome:GRCz11:4:68567783:68568233:-1 gene:ENSDARG00000110028.1 transcript:ENSDART00000184284.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX548011.1)

HSP 1 Score: 115.931 bits (289), Expect = 5.519e-30
Identity = 62/97 (63.92%), Postives = 70/97 (72.16%), Query Frame = 2

Query: 1391 IAFS*MKLSNNRSVMPLDVRGRTRATMAVTASFIPS*KWPGNLLNHCRVRNSGLKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLD 1681
            +AFS  +LSNNRSVMPLDVRG TRATM  +    P+ +  GN L   R  + GL+LF LNEEF VSA H  ALI SLPFVHTARRYYRL+G VRS D
Sbjct:   13 VAFSHARLSNNRSVMPLDVRGCTRATMGGSTCAYPAPRGAGNPLKPARDWDRGLQLFPLNEEFPVSAGHQPALIKSLPFVHTARRYYRLSGTVRSSD 109

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Match: CT583728.18 (pep chromosome:GRCz11:4:55794876:55795326:1 gene:ENSDARG00000114100.1 transcript:ENSDART00000189043.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CT583728.18)

HSP 1 Score: 115.931 bits (289), Expect = 5.914e-30
Identity = 62/97 (63.92%), Postives = 70/97 (72.16%), Query Frame = 2

Query: 1391 IAFS*MKLSNNRSVMPLDVRGRTRATMAVTASFIPS*KWPGNLLNHCRVRNSGLKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLD 1681
            +AFS  +LSNNRSVMPLDVRG TRATM  +    P+ +  GN L   R  + GL+LF LNEEF VSA H  ALI SLPFVHTARRYYRL+G VRS D
Sbjct:   13 VAFSHARLSNNRSVMPLDVRGCTRATMGGSTCAYPAPRGAGNPLKPARDWDRGLQLFPLNEEFPVSAGHQPALIKSLPFVHTARRYYRLSGTVRSSD 109

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Xenopus
Match: ENSXETT00000025836.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:88397208:88495673:1 gene:ENSXETG00000014084.1 transcript:ENSXETT00000025836.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 1.924e-10
Identity = 29/34 (85.29%), Postives = 29/34 (85.29%), Query Frame = 1

Query:  388 REPEKRLPHPRKAAGA*ITQYRFGEVVTKNNNMG 489
            REPEKRLPHPRKAAGA IT  R GEVVTKNNN G
Sbjct:   14 REPEKRLPHPRKAAGAQITHSRRGEVVTKNNNTG 47

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Match: A0A078A2G4 (Uncharacterized protein OS=Stylonychia lemnae OX=5949 GN=Contig11071.g11842 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 2.791e-57
Identity = 96/180 (53.33%), Postives = 111/180 (61.67%), Query Frame = -1

Query:  823 LEHKCTI*QVRVSFVNGINQTNRSTN*ERPCTTTHRIKKELSICQSSQCPDRVRFPVLSQIKPQAPLLVVPFRQFL*VSALQPYFPRNP*T*VSR*LLARRFELPSRTVSWHSLWSELGRYLIVFDPLTFVLD**KHSWQMLSQ*TVC**SKNFTSSTSIHMPPAVSFNHYFSFENQQNK 1362
            L +  T  Q +VSFVNGINQTN STN ERPCTTTHRIKKELSICQSS C D V FPVLSQI+PQAPLLVVPFRQFL VSAL+PY P+NP T +S        ++P    +   L S +G   IV+     V +                 S+NFTS   I MPP +  NHYFS  NQQ +
Sbjct: 1171 LNNGSTSQQAKVSFVNGINQTNHSTNQERPCTTTHRIKKELSICQSSLCLDLVSFPVLSQIEPQAPLLVVPFRQFLQVSALRPYSPQNPKTLISH-------KVPKADKA--RLRSLVG---IVYGQDQDVFN----------------KSENFTSDNQILMPPTIPINHYFSPSNQQRR 1322

HSP 2 Score: 63.5438 bits (153), Expect = 2.791e-57
Identity = 33/45 (73.33%), Postives = 36/45 (80.00%), Query Frame = -3

Query: 1523 QSVVATGGVYKGQGRNQRKLVTCAY*EFLVQVE*FQSTIPDTTVI 1657
            +SV ATGGVYKGQG NQRKL+TCAY EFLVQ   FQ +IP TT I
Sbjct: 1074 KSVGATGGVYKGQGLNQRKLMTCAYQEFLVQDLQFQRSIPITTHI 1118

HSP 3 Score: 40.817 bits (94), Expect = 2.791e-57
Identity = 16/22 (72.73%), Postives = 19/22 (86.36%), Query Frame = -1

Query: 1729 IIPQVHLRKPCYDFYFL*MIKF 1794
            I+PQVHLRKPCYDF FL ++ F
Sbjct: 1032 ILPQVHLRKPCYDFSFLQVLWF 1053

HSP 4 Score: 39.6614 bits (91), Expect = 2.791e-57
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -2

Query: 1416 ACGPGHLRASQTCYC 1460
            ACGPGHLRASQTCYC
Sbjct: 1139 ACGPGHLRASQTCYC 1153

HSP 5 Score: 62.3882 bits (150), Expect = 6.909e-7
Identity = 34/49 (69.39%), Postives = 36/49 (73.47%), Query Frame = -2

Query:  345 GPILLFFVTTSPNRYWVIYAPAAFRGCGSRFSGSLSGIEH*FPVTRYNH 491
            GP  LF VTTS ++ WVIYAPAAF  CGSRFSGSLSGIE    VTR  H
Sbjct: 1402 GP--LFLVTTSLSQDWVIYAPAAFLRCGSRFSGSLSGIEPQSSVTRERH 1448

HSP 6 Score: 21.9422 bits (45), Expect = 6.909e-7
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = -3

Query:  506 QLILDKVFKMFSLQ 547
            QLIL K FK +SLQ
Sbjct: 1382 QLILVKGFKFYSLQ 1395

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BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Match: J9HKN0 (Uncharacterized protein OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_14248 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.037e-54
Identity = 74/96 (77.08%), Postives = 79/96 (82.29%), Query Frame = -1

Query: 1096 SKKSAFALEHKCTI*QVRVSFVNGINQTNRSTN*ERPCTTTHRIKKELSICQSSQCPDRVRFPVLSQIKPQAPLLVVPFRQFL*VSALQPYFPRNP 1383
            SKKS   L +  T  Q +VSFVNGINQTN STN ERPCTTTHRIKKELSICQSS C D V FPVLSQI+PQAPLLVVPFRQFL VSAL+PY P+NP
Sbjct: 1242 SKKSC-PLSNGSTSQQAKVSFVNGINQTNHSTNQERPCTTTHRIKKELSICQSSLCLDLVSFPVLSQIEPQAPLLVVPFRQFLQVSALRPYSPQNP 1336

HSP 2 Score: 63.929 bits (154), Expect = 1.037e-54
Identity = 33/45 (73.33%), Postives = 36/45 (80.00%), Query Frame = -3

Query: 1523 QSVVATGGVYKGQGRNQRKLVTCAY*EFLVQVE*FQSTIPDTTVI 1657
            +SV ATGGVYKGQG NQRKL+TCAY EFLVQ   FQ +IP TT I
Sbjct: 1151 KSVGATGGVYKGQGLNQRKLMTCAYQEFLVQDLQFQRSIPITTHI 1195

HSP 3 Score: 41.5874 bits (96), Expect = 1.037e-54
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = -1

Query: 1729 IIPQVHLRKPCYDFYFL*MIKF 1794
            I+PQVHLRKPCYDF FL +I F
Sbjct: 1109 ILPQVHLRKPCYDFSFLQVIWF 1130

HSP 4 Score: 36.965 bits (84), Expect = 1.037e-54
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -2

Query: 1416 ACGPGHLRASQTCYC 1460
            ACGPGHLRASQTCYC
Sbjct: 1216 ACGPGHLRASQTCYC 1230

HSP 5 Score: 26.9498 bits (58), Expect = 1.037e-54
Identity = 16/36 (44.44%), Postives = 20/36 (55.56%), Query Frame = -2

Query: 1002 SPGTHRLRFPGDFSQGDLNYLRELSVGIVYGQN*DG 1109
            SP   +   P +  + D   LR L VGIVYGQ+ DG
Sbjct: 1332 SPQNPKTLNPQNPPKADKARLRSL-VGIVYGQDQDG 1366

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Match: J9I0R4 (Uncharacterized protein OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_13058 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.232e-53
Identity = 76/102 (74.51%), Postives = 82/102 (80.39%), Query Frame = -1

Query: 1078 SKKSAFALEHKCTI*QVRVSFVNGINQTNRSTN*ERPCTTTHRIKKELSICQSSQCPDRVRFPVLSQIKPQAPLLVVPFRQFL*VSALQPYFPRNP*T*VSR 1383
            SKKS   L +  T  Q +VSFVNGINQTN STN ERPCTTTHRIKKELSICQSS C D V FPVLSQI+PQAPLLVVPFRQFL VSAL+PY P+NP T +S 
Sbjct: 1242 SKKSC-PLSNGSTSQQAKVSFVNGINQTNHSTNQERPCTTTHRIKKELSICQSSLCLDLVSFPVLSQIEPQAPLLVVPFRQFLQVSALRPYSPQNPKTLISH 1342

HSP 2 Score: 63.929 bits (154), Expect = 5.232e-53
Identity = 33/45 (73.33%), Postives = 36/45 (80.00%), Query Frame = -3

Query: 1523 QSVVATGGVYKGQGRNQRKLVTCAY*EFLVQVE*FQSTIPDTTVI 1657
            +SV ATGGVYKGQG NQRKL+TCAY EFLVQ   FQ +IP TT I
Sbjct: 1151 KSVGATGGVYKGQGLNQRKLMTCAYQEFLVQDLQFQRSIPITTHI 1195

HSP 3 Score: 41.5874 bits (96), Expect = 5.232e-53
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = -1

Query: 1729 IIPQVHLRKPCYDFYFL*MIKF 1794
            I+PQVHLRKPCYDF FL +I F
Sbjct: 1109 ILPQVHLRKPCYDFSFLQVIWF 1130

HSP 4 Score: 36.965 bits (84), Expect = 5.232e-53
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -2

Query: 1416 ACGPGHLRASQTCYC 1460
            ACGPGHLRASQTCYC
Sbjct: 1216 ACGPGHLRASQTCYC 1230

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Match: A0A0R0GH50 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_13G012500 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.766e-46
Identity = 69/151 (45.70%), Postives = 78/151 (51.66%), Query Frame = 1

Query: 1243 LFLDSVGGGAWPFLVGGAICLVNSVNERDSNLLNSTFVF*RKC*LLRGINSV*LNEIEQ*QVCDALRCPGPHARYNGSNSEFYS*LEMAG*PAKSLSCQE*WIEIIPLERGIPSKRKSLACADYVPALCTHRPSLLPIEWFSEIVGF*FKE 1695
            LFLDS+GGGAWPFLVGGAICLVNSVNERD +LLNS         LLRG  ++                                                  I I+ L+RGIPSKR+S A  DYVPALCTHRPSLLPIEW  E+ G   +E
Sbjct:  235 LFLDSMGGGAWPFLVGGAICLVNSVNERDLSLLNSYVEVTLHGQLLRGTMAI--------------------------------------------------IAIVGLQRGIPSKRESSARVDYVPALCTHRPSLLPIEWSGEVFGLRRRE 335

HSP 2 Score: 88.5817 bits (218), Expect = 2.766e-46
Identity = 60/89 (67.42%), Postives = 67/89 (75.28%), Query Frame = 3

Query:  924 QSTAKAFAKNVFINQERKSEDRRRSDTVLVLTINYAN*QFAKVIQISLREVTGKPKXXXXXXXXVAKLKLKGIDGRAPPGVEPAA*FDS 1190
            ++TAKAFAK+VFINQERK   RRRSDTVLV TIN A+   A V   +      K KS+GSGGSMVA+LKLKGIDGRAPPGVEPA   DS
Sbjct:  151 RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPALFLDS 239

HSP 3 Score: 36.1946 bits (82), Expect = 2.766e-46
Identity = 24/59 (40.68%), Postives = 26/59 (44.07%), Query Frame = 1

Query:  718 WVLRMTDKFTLKKLECLKQAYACILLHGIIK*DFGFILLVFETEVMIKRDCRGHMYAGA 894
            W  R +   TLKKLEC KQ             D   I+L F   VMI RD RG   A A
Sbjct:  111 WPGRASGAVTLKKLECSKQ-------------DSDPIVLAFGIGVMINRDSRGRTTAKA 156

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Match: A0A3Q7YGQ3 (uncharacterized protein LOC113787721 OS=Cicer arietinum OX=3827 GN=LOC113787721 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.531e-46
Identity = 68/147 (46.26%), Postives = 76/147 (51.70%), Query Frame = 1

Query: 1243 LFLDSVGGGAWPFLVGGAICLVNSVNERDSNLLNSTFVF*RKC*LLRGINSV*LNEIEQ*QVCDALRCPGPHARYNGSNSEFYS*LEMAG*PAKSLSCQE*WIEIIPLERGIPSKRKSLACADYVPALCTHRPSLLPIEWFSEIVGF 1683
            LFLDS+GGGAWPFLVGGAICLVNSVNERD +LLNS         LLRG  ++                                                  I I+ L+RGIPSKR+S A  DYVPALCTHRPSLLPIEW  E+ G 
Sbjct:  156 LFLDSMGGGAWPFLVGGAICLVNSVNERDLSLLNSYVEVTLHGQLLRGTMAI--------------------------------------------------IAIVGLKRGIPSKRESSARVDYVPALCTHRPSLLPIEWSGEVFGL 252

HSP 2 Score: 86.6557 bits (213), Expect = 5.531e-46
Identity = 59/89 (66.29%), Postives = 66/89 (74.16%), Query Frame = 3

Query:  924 QSTAKAFAKNVFINQERKSEDRRRSDTVLVLTINYAN*QFAKVIQISLREVTGKPKXXXXXXXXVAKLKLKGIDGRAPPGVEPAA*FDS 1190
            ++TAK FAK+VFINQERK   RRRSDTVLV TIN A+   A V   +      K KS+GSGGSMVA+LKLKGIDGRAPPGVEPA   DS
Sbjct:   72 RTTAKTFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPALFLDS 160

HSP 3 Score: 39.2762 bits (90), Expect = 5.531e-46
Identity = 24/57 (42.11%), Postives = 26/57 (45.61%), Query Frame = 1

Query:  718 WVLRMTDKFTLKKLECLKQAYACILLHGIIK*DFGFILLVFETEVMIKRDCRGHMYA 888
            W  R     TLKKLEC KQ             D+G I+L F   VMI RD RG   A
Sbjct:   32 WPSRTFGAVTLKKLECSKQ-------------DYGPIVLAFGIGVMINRDSRGRTTA 75

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Match: EDO32480 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPZ7])

HSP 1 Score: 75.8702 bits (185), Expect = 2.472e-17
Identity = 41/50 (82.00%), Postives = 42/50 (84.00%), Query Frame = 2

Query: 1550 LKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNG 1699
            L+L  LNEEFLVSASH LALITSLPFVHTARRYYRLNGLVR   SD R G
Sbjct:    3 LQLLILNEEFLVSASHQLALITSLPFVHTARRYYRLNGLVRP--SDWRRG 50

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Match: EDO32586 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPZ7])

HSP 1 Score: 75.8702 bits (185), Expect = 2.472e-17
Identity = 41/50 (82.00%), Postives = 42/50 (84.00%), Query Frame = 2

Query: 1550 LKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNG 1699
            L+L  LNEEFLVSASH LALITSLPFVHTARRYYRLNGLVR   SD R G
Sbjct:    3 LQLLILNEEFLVSASHQLALITSLPFVHTARRYYRLNGLVRP--SDWRRG 50

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Match: EDO34225 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPZ7])

HSP 1 Score: 75.8702 bits (185), Expect = 2.472e-17
Identity = 41/50 (82.00%), Postives = 42/50 (84.00%), Query Frame = 2

Query: 1550 LKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNG 1699
            L+L  LNEEFLVSASH LALITSLPFVHTARRYYRLNGLVR   SD R G
Sbjct:    3 LQLLILNEEFLVSASHQLALITSLPFVHTARRYYRLNGLVRP--SDWRRG 50

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Match: EDO26390 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TC51])

HSP 1 Score: 75.8702 bits (185), Expect = 2.496e-17
Identity = 41/50 (82.00%), Postives = 42/50 (84.00%), Query Frame = 2

Query: 1550 LKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNG 1699
            L+L  LNEEFLVSASH LALITSLPFVHTARRYYRLNGLVR   SD R G
Sbjct:    3 LQLLILNEEFLVSASHQLALITSLPFVHTARRYYRLNGLVRP--SDWRRG 50

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Match: EDO32479 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUY8])

HSP 1 Score: 74.3294 bits (181), Expect = 8.122e-17
Identity = 40/50 (80.00%), Postives = 41/50 (82.00%), Query Frame = 2

Query: 1550 LKLFHLNEEFLVSASH*LALITSLPFVHTARRYYRLNGLVRSLDSDLRNG 1699
            L+L   NEEFLVSASH LALITSLPFVHTARRYYRLNGLVR   SD R G
Sbjct:    3 LQLLIFNEEFLVSASHQLALITSLPFVHTARRYYRLNGLVRP--SDWRRG 50

[Close]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Planmine SMEST
Match: SMESG000078439.1 (SMESG000078439.1)

HSP 1 Score: 54.299 bits (129), Expect = 2.139e-7
Identity = 27/30 (90.00%), Postives = 28/30 (93.33%), Query Frame = 2

Query: 1652 RLNGLVRSLDSDLRNGNISIIGRRQSNLII 1741
            RLNGLVRS DSDLR GNISIIGRRQSNLI+
Sbjct:  491 RLNGLVRSSDSDLREGNISIIGRRQSNLIM 520

The following BLAST results are available for this feature:

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Human
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Celegans
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Fly
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Xenopus
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Mouse
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. UniProt/SwissProt
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Cavefish
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Sea Lamprey
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Yeast
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Medaka
BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Planmine SMEST

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
zgc:158463	4.069e-36	60.00	zgc:158463 [Source:ZFIN;Acc:ZDB-GENE-070410-9]	[more]
AL935186.10	6.844e-31	65.98	pep chromosome:GRCz11:4:77561226:77561679:-1 gene:...	[more]
AL935186.6	6.844e-31	65.98	pep chromosome:GRCz11:CHR_ALT_CTG4_1_15:77561226:7...	[more]
BX548011.1	5.519e-30	63.92	pep chromosome:GRCz11:4:68567783:68568233:-1 gene:...	[more]
CT583728.18	5.914e-30	63.92	pep chromosome:GRCz11:4:55794876:55795326:1 gene:E...	[more]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSXETT00000025836.1	1.924e-10	85.29	pep primary_assembly:Xenopus_tropicalis_v9.1:7:883...	[more]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A078A2G4	2.791e-57	53.33	Uncharacterized protein OS=Stylonychia lemnae OX=5...	[more]
J9HKN0	1.037e-54	77.08	Uncharacterized protein OS=Oxytricha trifallax OX=...	[more]
J9I0R4	5.232e-53	74.51	Uncharacterized protein OS=Oxytricha trifallax OX=...	[more]
A0A0R0GH50	2.766e-46	45.70	Uncharacterized protein OS=Glycine max OX=3847 GN=...	[more]
A0A3Q7YGQ3	5.531e-46	46.26	uncharacterized protein LOC113787721 OS=Cicer arie...	[more]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO32480	2.472e-17	82.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32586	2.472e-17	82.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO34225	2.472e-17	82.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26390	2.496e-17	82.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32479	8.122e-17	80.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000078439.1	2.139e-7	90.00	SMESG000078439.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30032663 ID=SMED30032663|Name=Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2|organism=Schmidtea mediterranea sexual|type=transcript|length=1794bp
CTGGTTGATCCTGCCAGTAGTCATATGCTTATCTCAAAGATTAAGCCATG
CATGTCTTAGTACACAGACTAGTACTCTGAAACCGCGGATGGCTCATTAT
AACAGCTATGATTTGAGAGATTTTATCATTTTGCCACAAGATAACTGTGG
TAATTCTAGAGCTAATATTTTTAAGAATGCCGTGACTCACGAAGCGGCGG
ATTTATTAGATCAAAATCAACCCGCTGAAAGGCGGTATTTTGATGACTCT
GGATAATTTTACTGATCGTACGACCTTTGTGTTGACGACATATCTCTTGA
AGTGGCTGACCTATCAACTTTCGATGGTAAGATAAACGCTTACCATGGTT
GTAACGGGTAACGGGGAATCAGTGTTCGATTCCGGAGAGGGAGCCTGAGA
AACGGCTACCACATCCACGGAAGGCAGCAGGCGCGTAAATTACCCAATAC
CGGTTCGGTGAGGTAGTGACAAAAAATAACAATATGGGCCCTAGTGGTTT
CATAATTGCAATGAGAACATTTTAAATACTTTATCAAGTATCAATTGGAG
GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAAATTGAGGAAAAAGAT
GGTTTGAAATCTATGATTGAGAGCTATCTTGTCCTTCGAAGTCGTGTATA
TCTTTGATGCTCTTTAATGGGTGTTGAGGATGACCGACAAGTTTACTTTG
AAAAAATTAGAGTGCTTAAAGCAGGCTTACGCTTGTATATTGTTGCATGG
AATAATAAAATAGGACTTCGGTTTTATTTTGTTGGTTTTCGAAACTGAAG
TAATGATTAAAAGAGACTGCCGGGGGCATATGTATGCTGGTGCTAGAGGT
GAAATTCTTAGATCATCAGCAGACAGTCTACTGCGAAAGCATTTGCCAAG
AATGTTTTCATTAATCAAGAACGAAAGTCAGAGGATCGAAGACGATCAGA
TACCGTCCTAGTTCTGACCATAAACTATGCCAACTGACAGTTCGCGAAGG
TAATTCAAATCTCCTTGCGAGAAGTCACCGGGAAACCTAAGTCTATGGGT
TCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAG
GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAA
TCTCACCCGGTCCGGACACTGTGAGGATTGACAGATTGATAGCTCTTTCT
TGATTCGGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTT
GTCTGGTTAATTCCGTTAACGAACGAGACTCTAACCTGCTAAATAGTACA
TTTGTGTTCTAACGCAAATGCTGACTTCTTAGAGGAATAAATAGCGTTTA
GCTAAATGAAATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG
GGCCGCACGCGCGCTACAATGGCAGTAACAGCGAGTTTTATTCCTAGCTA
GAAATGGCCGGGTAACCTGCTAAATCACTGTCGTGTCAGGAATAGTGGAT
TGAAATTATTCCACTTGAACGAGGAATTCCTAGTAAGCGCAAGTCACTAG
CTTGCGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA
CCGATTGAATGGTTTAGTGAGATCGTTGGATTCTGATTTAAGGAATGGCA
ACATTTCTATAATTGGGAGAAGACAATCAAACTTGATCATTTAGAGGAAG
TAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGAT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000420	parapharyngeal region
PLANA:0000421	tail
PLANA:0002111	X2 cell

Vocabulary: **molecular function**
Term	Definition
GO:0003674	molecular_function

Vocabulary: **cellular component**
Term	Definition
GO:0005575	cellular_component

Vocabulary: **biological process**
Term	Definition
GO:0008150	biological_process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..17 score: 0.609

Main menu

Macaca fascicularis brain cDNA clone: QflA-20247, similar to human stathmin-like 2 (STMN2), mRNA, RefSeq: NM_007029.2

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Owlery

blazegraph-runner