Alkylated DNA repair protein alkB 1

Overview
NameAlkylated DNA repair protein alkB 1
Smed IDSMED30032654
Length (bp)1673
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Alkylated DNA repair protein alkB 1 (SMED30032654) t-SNE clustered cells

Violin plots show distribution of expression levels for Alkylated DNA repair protein alkB 1 (SMED30032654) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Alkylated DNA repair protein alkB 1 (SMED30032654) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Alkylated DNA repair protein alkB 1 (SMED30032654) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Human
Match: ALKBH1 (alkB homolog 1, histone H2A dioxygenase [Source:HGNC Symbol;Acc:HGNC:17911])

HSP 1 Score: 144.05 bits (362), Expect = 2.042e-37
Identity = 85/277 (30.69%), Postives = 142/277 (51.26%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLD--IEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE----------RLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            AY + G  GF +IPNPF        +   L  +    N  NLD  +  +   ++W+++ E    L +    K+   + L KLRW ++GYHY W  + Y  +  +  P++L  + + + +   +++F  EA I+NYY L+S +  H D +E     PL+S S GQ A++LLG       P  + ++ GD+++MSG SRL  HAVP+++  +PE       G  +  C+E           +VE   S E   +   Y++ +R+N+ +RQV+ T + FP
Sbjct:   90 AYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKE---FLRYKEATKRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDLGFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLP-NPE-------GEGLPHCLEAPLPAVLPRDSMVEPC-SMEDWQVCASYLKTARVNMTVRQVLATDQNFP 354
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Celegans
Match: alkb-1 (ALKylated DNA repair protein AlkB homolog [Source:UniProtKB/TrEMBL;Acc:E0DBL0])

HSP 1 Score: 115.161 bits (287), Expect = 7.594e-28
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 3 
Query:  402 LRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTP-IPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQV 935
            LRWT++G  Y W  + Y  N +  +P  L  +   I   LK  +  P+ATI+NYY   SA+  H D +E +   PL+S+SLGQ A+YL GT D  S P IPI L +GD ++M G  RL YHA+P I  I P+   N+  G  ++E  E      G+++    + +Y+  SR+N  +RQV
Sbjct:  183 LRWTTLGVEYDWNSKEYPPNGRP-VPEELYQLGNLISRSLKLGDMKPDATILNYYPPKSALSPHVDKSERSA-APLISMSLGQTAIYLSGTIDLSSEPPIPIWLRNGDFLIMHGDQRLVYHAIPCIGSI-PKRRGNR--GSMMDEDQEHFEPPRGNDDVDQSIIDYLNSSRVNFTIRQV 356
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Celegans
Match: alkb-1 (ALKylated DNA repair protein AlkB homolog [Source:UniProtKB/TrEMBL;Acc:E0DBL0])

HSP 1 Score: 115.161 bits (287), Expect = 7.594e-28
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 3 
Query:  402 LRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTP-IPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQV 935
            LRWT++G  Y W  + Y  N +  +P  L  +   I   LK  +  P+ATI+NYY   SA+  H D +E +   PL+S+SLGQ A+YL GT D  S P IPI L +GD ++M G  RL YHA+P I  I P+   N+  G  ++E  E      G+++    + +Y+  SR+N  +RQV
Sbjct:  183 LRWTTLGVEYDWNSKEYPPNGRP-VPEELYQLGNLISRSLKLGDMKPDATILNYYPPKSALSPHVDKSERSA-APLISMSLGQTAIYLSGTIDLSSEPPIPIWLRNGDFLIMHGDQRLVYHAIPCIGSI-PKRRGNR--GSMMDEDQEHFEPPRGNDDVDQSIIDYLNSSRVNFTIRQV 356
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Fly
Match: AlkB (gene:FBgn0065035 transcript:FBtr0074168)

HSP 1 Score: 140.198 bits (352), Expect = 6.565e-37
Identity = 92/274 (33.58%), Postives = 133/274 (48.54%), Query Frame = 3 
Query:  153 KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLD---IEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE-RLVEITGSEEKAN-IVWE----YIQKSRININMRQVVDTG 947
            K+   Y +    G   I NPF         A  L  +P   N +NL+    +    ++ WK+       L   S+G +        +RWT+ GYH+ W  ++Y    +S  P +LS +       L + +F PEA IVNYY + S +  H DH+E  K  PL S S GQ A++L+G       P  I L  GDV++MSG SRL YHAVP+I+K       + I+    N  I+ R ++     +  N   WE    Y+  SRININ+RQV++ G
Sbjct:   63 KDWRTYTLTNHPGIIVIRNPFSERGRRYWSARCLRDFPRTPNIVNLNERLFDESVRSDWWKQ-------LNLCSDGVEFQRI-KSAMRWTTFGYHHNWDTKIYDEEMQSPFPEDLSSLCGLFAQALGYADFKPEAAIVNYYPVGSTLSGHTDHSEPNKSAPLFSFSFGQTAIFLIGGRSLEEKPTAIYLQSGDVMIMSGESRLCYHAVPRIIKTQASATLSLIIEDVDNADIKTRTIDKDLFHDVGNPQFWEPFSRYMDDSRININIRQVLNPG 328
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Fly
Match: AlkB (gene:FBgn0065035 transcript:FBtr0445838)

HSP 1 Score: 140.198 bits (352), Expect = 6.565e-37
Identity = 92/274 (33.58%), Postives = 133/274 (48.54%), Query Frame = 3 
Query:  153 KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLD---IEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE-RLVEITGSEEKAN-IVWE----YIQKSRININMRQVVDTG 947
            K+   Y +    G   I NPF         A  L  +P   N +NL+    +    ++ WK+       L   S+G +        +RWT+ GYH+ W  ++Y    +S  P +LS +       L + +F PEA IVNYY + S +  H DH+E  K  PL S S GQ A++L+G       P  I L  GDV++MSG SRL YHAVP+I+K       + I+    N  I+ R ++     +  N   WE    Y+  SRININ+RQV++ G
Sbjct:   63 KDWRTYTLTNHPGIIVIRNPFSERGRRYWSARCLRDFPRTPNIVNLNERLFDESVRSDWWKQ-------LNLCSDGVEFQRI-KSAMRWTTFGYHHNWDTKIYDEEMQSPFPEDLSSLCGLFAQALGYADFKPEAAIVNYYPVGSTLSGHTDHSEPNKSAPLFSFSFGQTAIFLIGGRSLEEKPTAIYLQSGDVMIMSGESRLCYHAVPRIIKTQASATLSLIIEDVDNADIKTRTIDKDLFHDVGNPQFWEPFSRYMDDSRININIRQVLNPG 328
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Zebrafish
Match: alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:ZFIN;Acc:ZDB-GENE-050522-196])

HSP 1 Score: 152.525 bits (384), Expect = 3.379e-41
Identity = 106/332 (31.93%), Postives = 164/332 (49.40%), Query Frame = 3 
Query:   12 ESLLKPSYKKYKNRTYTLSELKELYNFTNPI------------KLNIEDSTLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIE---VDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVE-----ITGSEEKANIVWEYIQKSRININMRQVVDTG 947
            E   +  +K Y+ R     +  E+ NF+ P              L+ +D + +      K+  A+ + G  GF +I NPF        +   L  +P   N  NLD+    VD  N IW+ +I+ +       N K+  +T L KLRW ++GYHY W  + Y     +  P  L  +   + +   + EF  EA I+NYY  +S++  H D +E     PL+S S GQ AV+LLG +     P  + ++ GD++VMSGPSRL YHAVP IV  SP   A  +L   + + +E  V+      + S+E  +I   Y+Q SR+N+ +RQV+ +G
Sbjct:   10 EDAFRKLFKFYRRRN-PPPDFSEVINFSKPCNDKVFPCELNSTSLSEDDVSKVGLQPI-KDWRAFGLHGYPGFIFISNPFLPGSQQFWVKQCLKIYPQKPNVCNLDMHMSSVDTEN-IWERSIDAI---QRKGNRKREPKTLLEKLRWVTLGYHYDWNSKTYSPEHYTPFPKELQSLSHKVAAACGFSEFDAEAGILNYYRSDSSLGIHVDESELDHTRPLLSYSFGQTAVFLLGGTKREDRPTALFMHSGDIMVMSGPSRLHYHAVPCIVP-SP---AGNVLPPGLCQRLETEVQDEDVIQSVSQEDWDICSWYLQTSRVNVTVRQVLASG 331
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Xenopus
Match: slc35d3 (solute carrier family 35 member D3 [Source:Xenbase;Acc:XB-GENE-6067133])

HSP 1 Score: 159.458 bits (402), Expect = 6.285e-43
Identity = 90/288 (31.25%), Postives = 152/288 (52.78%), Query Frame = 3 
Query:  108 LNIEDSTLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKI-SPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFPN 962
            LN +D++        ++   Y + G  GF +I NPF        +   L  +P   N  NLD+ +  ++ +++W  + E+L      S  K+  ++ L KLRW ++GYHY W  + Y  +  S  P +L+++ + + +   +  F PEA I+NYYHL+S++  H D +E     PL+S S GQ  ++LLG  +    P P+ ++ GD++VMSG SRL YHAVP+I+     ++L   +      E  +  +    S     +  +Y+++SRIN+ +RQV+  G  FP 
Sbjct:   73 LNDQDASRAGLQPL-RQWKVYSLDGYPGFLFISNPFLPGWQHHWVGQCLKFYPRKPNVCNLDLHMTLEQTSDLWGSSQEQL---RSKSAEKRNKKSVLEKLRWVTLGYHYNWDTKTYNSDLHSPFPLDLAELSRCVSAACGFHNFKPEAGILNYYHLDSSLGIHVDESELDHQSPLLSFSFGQSCIFLLGGLNREHMPTPMMMHSGDIMVMSGQSRLLYHAVPRILPFPGGDMLPPCLSVPPATETSDPPLIEPCSTLDWEVCAQYLRRSRINMTVRQVLQEGCSFPQ 356
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Mouse
Match: Alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:MGI Symbol;Acc:MGI:2384034])

HSP 1 Score: 142.895 bits (359), Expect = 4.065e-37
Identity = 86/277 (31.05%), Postives = 142/277 (51.26%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE----------RLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            AY + G  GF +IPNPF        +   L  +    N  NLD  +  +    +W+++ E   +L      K+   + L +LRW ++GYHY W  + Y  +  +  P++L+ + + + +   ++ F  EA I+NYY L+S +  H D +E     PL+S S GQ A++LLG       P  + ++ GD++VMSG SRL  HAVP+++   P+       G  +  C+E           LVE   S E   +   Y++ +R+N+ +RQV+ TG++FP
Sbjct:   90 AYGLEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCNLDKHMTKEETQGLWEQSKE---VLRSKEVTKRRPRSLLERLRWVTLGYHYNWDSKKYSADHYTPFPSDLAFLSEQVATACGFQGFQAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVPRVLP-HPD-------GECLPHCLETPLPAVLPSNSLVEPC-SVEDWQVCATYLRTARVNMTVRQVLATGQDFP 354
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Mouse
Match: Alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:MGI Symbol;Acc:MGI:2384034])

HSP 1 Score: 103.605 bits (257), Expect = 3.822e-25
Identity = 61/198 (30.81%), Postives = 95/198 (47.98%), Query Frame = 3 
Query:  192 FYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIV 779
            F +IPNPF        +   L  +    N  NLD  +  +    +W+++ E   +L      K+   + L +LRW ++GYHY W                     K + +   ++ F  EA I+NYY L+S +  H D +E     PL+S S GQ A++LLG       P  + ++ GD++VMSG SRL  HAVP+++
Sbjct:    2 FIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCNLDKHMTKEETQGLWEQSKE---VLRSKEVTKRRPRSLLERLRWVTLGYHYNWDS-------------------KQVATACGFQGFQAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVPRVL 177
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Mouse
Match: Alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:MGI Symbol;Acc:MGI:2384034])

HSP 1 Score: 48.521 bits (114), Expect = 5.654e-6
Identity = 31/110 (28.18%), Postives = 50/110 (45.45%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNL 488
            AY + G  GF +IPNPF        +   L  +    N  NLD  +  +    +W+++ E   +L      K+   + L +LRW ++GYHY W  +      KS +P + 
Sbjct:   90 AYGLEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCNLDKHMTKEETQGLWEQSKE---VLRSKEVTKRRPRSLLERLRWVTLGYHYNWDSK-----SKSPLPVDF 191
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Match: sp|Q13686|ALKB1_HUMAN (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 144.05 bits (362), Expect = 9.808e-37
Identity = 85/277 (30.69%), Postives = 142/277 (51.26%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLD--IEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE----------RLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            AY + G  GF +IPNPF        +   L  +    N  NLD  +  +   ++W+++ E    L +    K+   + L KLRW ++GYHY W  + Y  +  +  P++L  + + + +   +++F  EA I+NYY L+S +  H D +E     PL+S S GQ A++LLG       P  + ++ GD+++MSG SRL  HAVP+++  +PE       G  +  C+E           +VE   S E   +   Y++ +R+N+ +RQV+ T + FP
Sbjct:   90 AYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKE---FLRYKEATKRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDLGFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLP-NPE-------GEGLPHCLEAPLPAVLPRDSMVEPC-SMEDWQVCASYLKTARVNMTVRQVLATDQNFP 354
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Match: sp|P0CB42|ALKB1_MOUSE (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.843e-36
Identity = 86/277 (31.05%), Postives = 142/277 (51.26%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIE----------RLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            AY + G  GF +IPNPF        +   L  +    N  NLD  +  +    +W+++ E   +L      K+   + L +LRW ++GYHY W  + Y  +  +  P++L+ + + + +   ++ F  EA I+NYY L+S +  H D +E     PL+S S GQ A++LLG       P  + ++ GD++VMSG SRL  HAVP+++   P+       G  +  C+E           LVE   S E   +   Y++ +R+N+ +RQV+ TG++FP
Sbjct:   90 AYGLEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCNLDKHMTKEETQGLWEQSKE---VLRSKEVTKRRPRSLLERLRWVTLGYHYNWDSKKYSADHYTPFPSDLAFLSEQVATACGFQGFQAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVPRVLP-HPD-------GECLPHCLETPLPAVLPSNSLVEPC-SVEDWQVCATYLRTARVNMTVRQVLATGQDFP 354
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Match: sp|Q54N08|ALKB_DICDI (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 2.588e-31
Identity = 86/286 (30.07%), Postives = 131/286 (45.80%), Query Frame = 3 
Query:  153 KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL*LLYFWSNGKKLSE--------------------TWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGK 950
            +E   Y + G  GFY+I +PF      + I  +L  +    N  N+ +       +WK   +EL      S GK   +                      L KL W+++GY YQWT R+Y        P +L ++++ I    K+  ++ EA  VN+Y  +S M  H D AE   + P++SIS G  AV+L+G       P+P+ +  GD+V+M G SR  YH V KIV+ S +L       G ++E  ++ +       K  I W   +  R+NIN RQV    K
Sbjct:  112 REWKVYGLQGYPGFYFIKSPFTASQQKKWIKHALEDYADPPNNNNITLFHGPIKNLWKNGEKELINEELKSQGKHDDDEIEQPTRPLDKNGEPLPTYRQLLDKLAWSTLGYQYQWTPRLYSEEFYEEFPDDLQELVQKIAIATKFDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAVFLMGAETRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIVENSFDL-------GLIDENDDQDL-------KYKIQWLKEKNRRVNINTRQVFKVPK 383
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Match: sp|Q9SA98|ALKBH_ARATH (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 119.398 bits (298), Expect = 2.520e-28
Identity = 107/340 (31.47%), Postives = 153/340 (45.00%), Query Frame = 3 
Query:    3 YEPESLLKPSYKKYKNRTYTLSELKEL----YNFTNPIKL--NIEDSTLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLD-------------------IEVDRPNEIWKETIEEL*LLYFWSNGKKLSET-WLYKLRWTSMGYHYQWTDRVYKRNC-KSNIPTNLSDIMKD--IISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQV 935
            YE +S  K S KK   +   LSEL +      NF N   L   I  S + +   FC       I  + GFY+IP+        + I +SLT +P   N+ N +                   ++ D  N  WK   E        S+ K +S +  L KLRW+++G  + W+ R Y  +   +NIP  L  + K    I++   +EF PE  IVNY+ +   +  H D  E     P+VS+SLG  A++LLG       P  + L  GDVV+M+G +R  +H +P+            I  G  N  I  L E   S E  +   EYI+ SRININ+RQV
Sbjct:   27 YEQDS--KFSRKKKLPKPIDLSELLDFNLISQNFNNDGVLPDGIRVSKVDSSPVFC-------IDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNHNAIYGPIDDLFDSAKENKVLVQDDLTNNKWKFYEEVDIEKATRSSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGEEFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYLRSGDVVLMAGEARECFHGIPR------------IFTGEENADIGAL-ESELSHESGHFFAEYIKTSRININIRQV 344
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Match: sp|O60066|ALKBH_SCHPO (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 114.005 bits (284), Expect = 8.458e-27
Identity = 95/332 (28.61%), Postives = 146/332 (43.98%), Query Frame = 3 
Query:    6 EPESLLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDSTLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSL-TKWPTNVNKMNLDIEVDRP---NEIWKETIEEL*LLYFWSNGKK----LSETWLY--------KLRWTSMGYHYQWTDRVYKRNCKS-NIPTNLSD----IMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWE-YIQKSRININMRQV 935
            E  ++ +   K+YK R  T+ ++ E+ +  +P     E    I P  F  +          G   + N    ++ +QL+   + T+     NK NL      P   + IW+         Y+  +G+     L ET           KLRW ++G  Y WT + Y    KS   P +L D    ++K+    L WK    EA IVN+Y     +  H D +E    +PL+S+S+G   +YL+GT      P  + L+ GDVV+M+G SR A+HAVPKI+   P    N +L G                   N  W+ +I + R+N N+RQV
Sbjct:    7 EQANVFRLEEKRYKCRADTIPDMSEVLDPNDPQSFGFEALVEIKPRVFSFQKAP-------GLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFYQLPLGNDSIWRR--------YYNGDGESIIDGLGETKPLTVDRLVHKKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDFLHWKA---EAAIVNFYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTGTSRKAFHAVPKII---PNSTPNYLLTG-------------------NKAWDGWISRKRVNFNVRQV 298
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Match: A0A093CQR5 (Alkylated DNA repair protein alkB 1 (Fragment) OS=Tauraco erythrolophus OX=121530 GN=N340_05156 PE=4 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 5.085e-44
Identity = 99/337 (29.38%), Postives = 177/337 (52.52%), Query Frame = 3 
Query:   12 ESLLKPSYKKYKNRTYTLSELKELYNFTNP----IKLNIEDSTLINPNAF------CKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV--DRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEIT---------GSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            E   +  ++ Y+ R   +S+L+ + +F+ P     +  +  S+L + +A+        +  AY + G  GF +IPNPF        +   L  +P   N  NLD+ +  ++  ++W ++ E+L       + K+   + L KLRW ++GYHY W  + Y  N  +  P++L+ + + + +   ++ F  +A I+NYYH +S++  H D +E     PL+S S GQ +++LLG       P  + ++ GD++VMSG SRL YHAVP++   +PE       G A+  C+E+ + +           S+E   +  +Y+Q SRIN+ +RQV+  G++FP
Sbjct:   11 EDAFRRLFRFYRQRD--ISDLRGVVDFSVPGSQVFRSQLSISSLSDQDAYRAGLQPVSQWKAYGLNGYPGFIFIPNPFLPGCQRHWVKQCLKLYPQKPNVCNLDLHMAPEKTIDLWGQSKEQL---RRKGSSKQEPRSLLEKLRWVTLGYHYNWDTKKYSANHHTPFPSDLAFLSEQVATACGFRGFQAQAGILNYYHFDSSLGIHADESELDHSRPLLSFSFGQSSIFLLGGLKREEAPTAMFMHSGDIMVMSGFSRLRYHAVPRVFP-NPE-------GTALPSCLEQALSLDLPVGSVIEHSSDEDWQVCVKYLQSSRINMTIRQVLAEGQKFP 334
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Match: I3K0T0 (AlkB homolog 1, histone H2A dioxygenase OS=Oreochromis niloticus OX=8128 PE=4 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 6.007e-44
Identity = 96/277 (34.66%), Postives = 145/277 (52.35%), Query Frame = 3 
Query:  153 KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVD--RPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITG------SEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            +E  A+ + G  GF +I NPF        +   L  +P   N  NLD+ +      +IW +++  L        GK+ + T L +LRW ++GYHY W  + Y  +  +  P +L  +   + +   +  FI EA I+NYY  +S++  H D +E     PL+S S GQ A++LLG       P  + ++ GDV+VMSGPSRL YHAVP+I+   PE LA +  G     CI+      G      SEE+  +   YIQ SR+N+ +RQV+  G++FP
Sbjct:   71 REWQAFGLRGHPGFIFISNPFLRGTQPFWLRQCLKIYPQKPNICNLDMHMSPLETQDIWGQSVHSL---RSSQAGKREARTLLERLRWVTLGYHYNWDTKTYSPSSHTPFPRDLHLLSAHVTAACGFPGFISEAGILNYYRSDSSLGIHVDESELDHTRPLLSFSFGQSAIFLLGGPRRQDPPTAMLMHSGDVMVMSGPSRLLYHAVPRILPAPPERLALETGG-----CIQAPPPQEGSVVEPVSEEEWAVCSRYIQSSRVNVTVRQVLAPGQKFP 339
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Match: A0A210QNU8 (Alkylated DNA repair protein alkB-like 1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09433 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 9.850e-44
Identity = 102/334 (30.54%), Postives = 161/334 (48.20%), Query Frame = 3 
Query:   21 LKPSYKKYKNRTYTLS-----ELKELYNFTNPIKLNIED---STLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPN-EIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPE------------LLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
             KP +KKYK R   L      +  +  N     K  +ED   S L      C E  AY +    GF +I NPF        ++  +  +P   N  NLD  +D  +  IW   I          +     E+ + KLRW +MGYHY W  + Y ++ K+    ++S + + + S + + +F PEA ++NYYHL+S++  H DH+EF    PL+S S GQ A++L+G     + P  + L  GD+ VMS  +RL YHAVP+I++  P+            L +      + +    R V         +++ +Y+ ++RIN N+RQV+   +EFP
Sbjct:   17 FKPIFKKYKRRDRPLDVSDVIDFDKGNNLDKVEKFKLEDTGQSLLTGLKPVC-EWKAYCLKSNPGFLFIVNPFDNGYQRYWVSRCVKDYPCRPNVRNLDAYMDVGDVNIWDTAIST------HKDDITDKESLMMKLRWVTMGYHYHWGTKKYYKDSKAPFQEDMSRLGRYVASAIGYPKFSPEAAVLNYYHLDSSLSGHTDHSEFDHSAPLISFSFGQDAIFLIGGETKETEPSAVFLRSGDICVMSASARLVYHAVPRIMETDPQRNHRCFDLSDIKLDSESCTSASCDSAAHRHVVERLHSNDWDLMSKYLSQTRINFNIRQVLVPDREFP 343
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Match: A0A401PFP0 (Fe2OG dioxygenase domain-containing protein OS=Scyliorhinus torazame OX=75743 GN=scyTo_0001759 PE=4 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 1.424e-43
Identity = 93/273 (34.07%), Postives = 148/273 (54.21%), Query Frame = 3 
Query:  162 TAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRP--NEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIV-----KISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            T +   G  GF +I +PF        +   L  +P   N  NLD+ + R    ++W+++ E+L L    + GK+ +++ L KLRW ++GYHY W  + Y  +  +  PT+L+ + + + +   +++F  EA I+NYYH +S++  H D +E     PL+S S GQ AV+LLG       P+P+ ++ GD+V+MS  SRL YHAVP+IV     +  P  L N  +    + C          E+   I  +Y+Q SRIN+ +RQVV  G+ FP
Sbjct:   19 TLHYFPGVGGFMFISDPFLPGWQRYWVKQCLKIYPRKPNVCNLDLHMSREETTDLWEQSREQLRLK---APGKREAKSLLEKLRWVTLGYHYNWETKKYSPDHHTTFPTDLAHLSEQVAAACGFQQFKAEAGILNYYHFDSSLGIHVDESEMDYSKPLLSFSFGQSAVFLLGGLKRQDPPLPMFIHSGDIVIMSDRSRLFYHAVPRIVPNLEGRAVPSCLENTPIR---DNCSADTSYQQSDEDDWEICMKYLQSSRINMTVRQVVRVGQSFP 285
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Match: R7U9X0 (Fe2OG dioxygenase domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_191192 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 2.346e-43
Identity = 101/269 (37.55%), Postives = 148/269 (55.02%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDI---EVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKI-SPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            AY+     GFY+I NPF  D   + I   L  +P   N  NLD+   ++ R   +W         ++  ++     +  L+KLRW+++GYH+ W  + Y  +  +  P +LS + + I   + +  F  EA IVNYYHL+S +  H DH+EF    PL+SIS GQ AV+LLG       PI + L+ GD+ +MSG  RLAYHAVPKI++  + EL  ++     VN    R VE T    ++     Y+Q SRIN+N+RQV+  G+EFP
Sbjct:   60 AYQHRKIPGFYFISNPFTPDGQRKWIKRCLNDFPLKPNITNLDVNYSDLIRERNVWS--------MHLDADSTDEEKQLLHKLRWSTLGYHHNWDTKKYTADRYTPFPDDLSCLSRCIAHGIGFPHFKAEAAIVNYYHLDSTLSGHTDHSEFDHISPLISISFGQTAVFLLGGLTKDIDPIALYLHSGDICIMSGECRLAYHAVPKILRTPTSELPYHEGDDDTVN---GRKVEDTFEPFES-----YLQSSRINMNVRQVLCDGQEFP 312
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Cavefish
Match: alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:NCBI gene;Acc:103037170])

HSP 1 Score: 144.05 bits (362), Expect = 1.093e-37
Identity = 87/273 (31.87%), Postives = 133/273 (48.72%), Query Frame = 3 
Query:  153 KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNE---IWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITG-SEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            K+  A+ +    GF +I NPF        +   L  +P   N  NLD+ +  P E   IW+ +++ +         K   +T L KLRW ++GYHY W  + Y  +  +  P +L  + + + +   +  F  EA I+NYY  +S++  H D +E     PL+S S GQ AV+LLG +     P  + +  GD++VMSG SRL YHAVP I+           L     +       I G SEE   +   Y+  SR+N+ +RQV+  G+ FP
Sbjct:   72 KDWRAFSLQTHPGFLFISNPFLPGSQQHWVKQCLKAYPQKPNVCNLDMHMS-PAETENIWERSVDSI-----RKKEKSRPKTLLEKLRWVTLGYHYNWDTKTYSADHFTPFPQDLHSLSETVAAACGFPGFKSEAGILNYYRSDSSLGIHVDESELNHTFPLLSYSFGQTAVFLLGGTKRQDPPTAMFMRSGDIMVMSGSSRLLYHAVPCIIPAPAAQPLPPCLDQRQEKQPVSGGIIQGVSEEDWEVCSRYLLTSRVNMTVRQVLGAGQTFP 338
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Sea Lamprey
Match: alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:ZFIN;Acc:ZDB-GENE-050522-196])

HSP 1 Score: 48.9062 bits (115), Expect = 5.633e-7
Identity = 31/97 (31.96%), Postives = 43/97 (44.33%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL*LLYFWSNGKKLSE--TWLYKLRWTSMGYHYQWTDRV 449
            AY + G  GF +I NPF        +   L  +P   N  NLD    + +++W   I           GK   E  + L KLRW ++GYHY W  +V
Sbjct:   90 AYGLNGYPGFIFIVNPFLPSGQRFWVRQCLKLYPRKPNVCNLDAHDPQRDDVWASGISSP------RAGKSPREPRSLLEKLRWVTLGYHYNWDTKV 180
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Nematostella
Match: EDO43822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXQ8])

HSP 1 Score: 125.561 bits (314), Expect = 4.704e-32
Identity = 90/327 (27.52%), Postives = 152/327 (46.48%), Query Frame = 3 
Query:   18 LLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDS-----TLINPN---AFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKIS-----PELLANQILGGAVNECIERLVEITGSEEKANIVW----EYIQKSRININMRQVVDTG 947
            L++  YK+YK R     +  E+ +F +P     + S     +L + +      ++  AY +  + G  +I NPF            L  +P   N  NLD  +         +I +   ++  SN + ++   + +LRW  +GY + +    YK       P +L  +M  +   + +  + PEA IVNYY L+++M  H DH E     PL+S+S GQ AV+L+G       P  + +  GD+++MSG +RLA+HAVP+I+++      PE           + C      +T S       W    +Y+ ++RIN+N+RQV   G
Sbjct:   16 LIRQEYKRYK-RKKPPPDFSEVIDFNDPQTFQGKVSEWPLKSLPHSDFGLKCARQWRAYHLDCRPGLIFIVNPFVSGAQHYWARRCLEDFPRKPNISNLDAHM---------SIGDDDCIWALSNSEHVN--LIDRLRWVHLGYQFDYNVVDYKPEKYYGFPKDLGGLMHHLAEAIGYLGYTPEAGIVNYYPLSASMGGHTDHYELDLSWPLISVSFGQSAVFLIGGKTKDVKPTALYIRSGDILIMSGEARLAFHAVPRIIQVQTKDNIPE-----------DSCCNPKAFLTQSPPMTEEEWLPFAKYLSRTRINVNVRQVHSLG 319
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Medaka
Match: alkbh1 (alkB homolog 1, histone H2A dioxygenase [Source:NCBI gene;Acc:101161845])

HSP 1 Score: 150.599 bits (379), Expect = 3.942e-40
Identity = 89/269 (33.09%), Postives = 137/269 (50.93%), Query Frame = 3 
Query:  165 AYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPN--EIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKI--SPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFP 959
            A+ + G  GF +I NPF        +   L  +P   N  NLD+ +   +  +IW ++     +L   S+G+   +T L KL W ++GYHY W  + Y  +  +  P +L  +   + S   +  F  EA I+NYY  +S++  H D +E     PL+S S GQ A++LLG +     P  + ++ GDV+VMSG SRL YHAVP+I+        L   +   +  +    L  +  SEE       YIQKSR+N+ +RQV+  G+ FP
Sbjct:   75 AFSLQGFPGFIFISNPFLRGSQPFWVRQCLKTYPQKPNVCNLDLHMSACDTQDIWAKSAP---VLRSPSSGRNQPKTLLEKLHWVTLGYHYNWDTKTYSADNHTPFPADLHSLSARVASACGFPGFRSEAAILNYYRPDSSLGIHVDESELDHTRPLLSFSFGQSAIFLLGGTRRQDPPTAMLVHSGDVMVMSGQSRLLYHAVPRILPTPRGHGALLEDLRPASPQQAAAMLEPV--SEEDWMACSVYIQKSRLNMTVRQVLGAGQSFP 338
[Close]
BLAST of Alkylated DNA repair protein alkB 1 vs. Planmine SMEST
Match: SMESG000035430.1 (SMESG000035430.1)

HSP 1 Score: 634.795 bits (1636), Expect = 0.000e+0
Identity = 311/320 (97.19%), Postives = 313/320 (97.81%), Query Frame = 3 
Query:    3 YEPESLLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDSTLINPNAFCKEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL*LLYFWSNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFPN 962
            YEPESLLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDSTLINPNAFCKEVTAYEIG KSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL      SNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLG+SDPLSTP+PITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFPN
Sbjct:    3 YEPESLLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDSTLINPNAFCKEVTAYEIGAKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEVDRPNEIWKETIEEL------SNGKKLSETWLYKLRWTSMGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYYHLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGSSDPLSTPLPITLNHGDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGSEEKANIVWEYIQKSRININMRQVVDTGKEFPN 316
The following BLAST results are available for this feature:
BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ALKBH12.042e-3730.69alkB homolog 1, histone H2A dioxygenase [Source:HG... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
alkb-17.594e-2839.11ALKylated DNA repair protein AlkB homolog [Source... [more]
alkb-17.594e-2839.11ALKylated DNA repair protein AlkB homolog [Source... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AlkB6.565e-3733.58gene:FBgn0065035 transcript:FBtr0074168[more]
AlkB6.565e-3733.58gene:FBgn0065035 transcript:FBtr0445838[more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh13.379e-4131.93alkB homolog 1, histone H2A dioxygenase [Source:ZF... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
slc35d36.285e-4331.25solute carrier family 35 member D3 [Source:Xenbase... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Alkbh14.065e-3731.05alkB homolog 1, histone H2A dioxygenase [Source:MG... [more]
Alkbh13.822e-2530.81alkB homolog 1, histone H2A dioxygenase [Source:MG... [more]
Alkbh15.654e-628.18alkB homolog 1, histone H2A dioxygenase [Source:MG... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q13686|ALKB1_HUMAN9.808e-3730.69Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX... [more]
sp|P0CB42|ALKB1_MOUSE2.843e-3631.05Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX... [more]
sp|Q54N08|ALKB_DICDI2.588e-3130.07Alpha-ketoglutarate-dependent dioxygenase alkB OS=... [more]
sp|Q9SA98|ALKBH_ARATH2.520e-2831.47Alpha-ketoglutarate-dependent dioxygenase alkB OS=... [more]
sp|O60066|ALKBH_SCHPO8.458e-2728.61Alpha-ketoglutarate-dependent dioxygenase abh1 OS=... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A093CQR55.085e-4429.38Alkylated DNA repair protein alkB 1 (Fragment) OS=... [more]
I3K0T06.007e-4434.66AlkB homolog 1, histone H2A dioxygenase OS=Oreochr... [more]
A0A210QNU89.850e-4430.54Alkylated DNA repair protein alkB-like 1 OS=Mizuho... [more]
A0A401PFP01.424e-4334.07Fe2OG dioxygenase domain-containing protein OS=Scy... [more]
R7U9X02.346e-4337.55Fe2OG dioxygenase domain-containing protein OS=Cap... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh11.093e-3731.87alkB homolog 1, histone H2A dioxygenase [Source:NC... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh15.633e-731.96alkB homolog 1, histone H2A dioxygenase [Source:ZF... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO438224.704e-3227.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh13.942e-4033.09alkB homolog 1, histone H2A dioxygenase [Source:NC... [more]
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BLAST of Alkylated DNA repair protein alkB 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000035430.10.000e+097.19SMESG000035430.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032654 ID=SMED30032654|Name=Alkylated DNA repair protein alkB 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1673bp
CTTATGAACCTGAATCATTATTGAAGCCATCTTACAAGAAATATAAAAAT
CGAACTTATACTTTATCAGAATTAAAAGAACTTTACAATTTTACAAATCC
TATAAAACTCAACATTGAAGATTCAACTTTAATAAATCCTAATGCCTTTT
GTAAAGAAGTCACTGCTTATGAAATTGGCGGAAAAAGCGGATTCTATTAT
ATTCCAAACCCATTCAAAGGTGACATTTTAATTCAGTTAATTGCCGATTC
TCTCACGAAATGGCCTACAAATGTAAATAAAATGAATTTGGATATCGAGG
TCGATAGACCTAATGAAATTTGGAAAGAAACCATCGAAGAATTGTAATTA
TTATATTTTTGGAGTAATGGTAAAAAATTATCTGAAACTTGGCTTTATAA
ATTACGTTGGACATCTATGGGATATCACTATCAATGGACAGATAGAGTAT
ATAAGAGAAATTGTAAATCTAATATTCCCACCAACTTGTCAGATATTATG
AAAGATATAATTTCAATTTTGAAATGGAAAGAATTTATACCCGAAGCAAC
AATAGTCAATTATTATCATTTAAATTCAGCCATGTGCTTTCATCAGGATC
ATGCTGAATTTGCAAAGGATGTGCCATTGGTATCTATAAGTTTGGGCCAA
CCGGCGGTGTATTTATTGGGCACAAGTGATCCTTTGTCAACTCCAATACC
GATAACTCTCAATCACGGAGATGTTGTCGTCATGTCGGGACCGTCGCGAC
TTGCTTATCACGCTGTACCGAAAATAGTGAAAATAAGCCCAGAACTATTG
GCCAATCAAATATTAGGTGGTGCCGTAAACGAGTGCATAGAACGATTAGT
GGAAATTACCGGATCCGAGGAGAAGGCAAATATCGTTTGGGAGTACATCC
AAAAGAGTCGAATAAACATTAATATGAGACAAGTTGTTGACACTGGAAAA
GAGTTTCCTAATTGAATAAAATGATTTTATTTTTTGGAATTTTCATTGTT
TATCGATTTTCTTACAATTTTCCAACGGATTAACACTACCGATGGTATCG
GGAGTGGAATCTAGTCTAGCGGTTGATTTGTGATGTTGTCCAGCGTTCTT
AATGTCAATATGATCTAAAATGGCAATTTAGTATGGGGAACATTGGCGCA
TTGTTGTACAGGATCACCGATTATGAAATATTAAACGCGGGCTCGTTTAT
GCAGCTTTGATATTTACTCATATGTACTGTTAGCGCTCAAGGGGGTTTCT
AAGGTTTGTTTACGTTGCCTATAGCAACTGCTTCAATGGGCACCGAAACC
ATTGGTTACATAACACCCAATCCATATATGGAGGTGTTACTCTATTAATT
ACTCTCTAACTAGCTACCAATTAATTCTTCCCATCTTAATACTTCTCGCT
CGGTGATGGTCATTTCAATGTCTTTGTTGATTGGGCGGTTATTGCACGGT
CGTTTGGTCGTCCACAGTGAATATTCTGGAAACTGGGAAAACGAACTTGG
ATTTGATCCTAACTAATTATCAGAAGAATCGCGAGATCGAAACAATAGTG
CTATTATGAACAAAATTTAAGCAAGGCTTGAAATCTACCAATAAAACACG
ATGCCGATCCTATAATGCGCAAAATACAATCTACAATAAAATCTACAAAT
AAAAATAAAAAAATACAATTAAA
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protein sequence of SMED30032654-orf-1

>SMED30032654-orf-1 ID=SMED30032654-orf-1|Name=SMED30032654-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
YEPESLLKPSYKKYKNRTYTLSELKELYNFTNPIKLNIEDSTLINPNAFC
KEVTAYEIGGKSGFYYIPNPFKGDILIQLIADSLTKWPTNVNKMNLDIEV
DRPNEIWKETIEEL*
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protein sequence of SMED30032654-orf-2

>SMED30032654-orf-2 ID=SMED30032654-orf-2|Name=SMED30032654-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=183bp
MGYHYQWTDRVYKRNCKSNIPTNLSDIMKDIISILKWKEFIPEATIVNYY
HLNSAMCFHQDHAEFAKDVPLVSISLGQPAVYLLGTSDPLSTPIPITLNH
GDVVVMSGPSRLAYHAVPKIVKISPELLANQILGGAVNECIERLVEITGS
EEKANIVWEYIQKSRININMRQVVDTGKEFPN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR037151AlkB-like_sf
IPR004574Alkb
IPR027450AlkB-like
IPR005123Oxoglu/Fe-dep_dioxygenase
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006281DNA repair
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 2..171
e-value: 1.1E-29
score: 104.0
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3DG3DSA:2.60.120.590coord: 1..175
e-value: 6.7E-37
score: 129.2
IPR004574Alkylated DNA repair protein AlkBPANTHERPTHR16557ALKYLATED DNA REPAIR PROTEIN ALKB-RELATEDcoord: 1..174
NoneNo IPR availablePANTHERPTHR16557:SF2NUCLEIC ACID DIOXYGENASE ALKBH1coord: 1..174
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 2..174
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 41..174
score: 8.406