Fucolectin

Overview
NameFucolectin
Smed IDSMED30032543
Length (bp)344
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Fucolectin (SMED30032543) t-SNE clustered cells

Violin plots show distribution of expression levels for Fucolectin (SMED30032543) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032543

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30032543SMESG000014445.1 SMESG000014428.1 Contig43488newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032543SMESG000014445.1 SMESG000014428.1 Contig43488uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Fucolectin vs. Ensembl Zebrafish
Match: si:ch73-359m17.2 (si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-90])

HSP 1 Score: 46.595 bits (109), Expect = 5.278e-7
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN      K  C+HT+++  PWW VDL G  ++  V I+NR +    RI GA
Sbjct:   50 GNTEADYRKGSCTHTSREFNPWWRVDLGGVSSVNKVTITNRGDCCEERIRGA 101
[Close]
BLAST of Fucolectin vs. Ensembl Zebrafish
Match: si:cabz01040626.2 (si:cabz01040626.2 [Source:ZFIN;Acc:ZDB-GENE-160113-147])

HSP 1 Score: 47.3654 bits (111), Expect = 6.380e-7
Identity = 20/41 (48.78%), Postives = 27/41 (65.85%), Query Frame = 3 
Query:   87 CSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            C+HT   + PWW +DLQ SY++  V I+NR +  S RI GA
Sbjct:   55 CTHTATQTLPWWRLDLQKSYSVNRVSITNRLDCCSERINGA 95
[Close]
BLAST of Fucolectin vs. Ensembl Zebrafish
Match: si:cabz01040626.2 (si:cabz01040626.2 [Source:ZFIN;Acc:ZDB-GENE-160113-147])

HSP 1 Score: 46.9802 bits (110), Expect = 7.041e-7
Identity = 20/41 (48.78%), Postives = 27/41 (65.85%), Query Frame = 3 
Query:   87 CSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            C+HT   + PWW +DLQ SY++  V I+NR +  S RI GA
Sbjct:   58 CTHTATQTLPWWRLDLQKSYSVNRVSITNRLDCCSERINGA 98
[Close]
BLAST of Fucolectin vs. Ensembl Zebrafish
Match: si:ch73-359m17.2 (si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-90])

HSP 1 Score: 46.2098 bits (108), Expect = 1.086e-6
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN      K  C+HT+++  PWW VDL G  ++  V I+NR +    RI GA
Sbjct:   50 GNTEADYRKGSCTHTSREFNPWWRVDLGGVSSVNKVTITNRGDCCEERIRGA 101
[Close]
BLAST of Fucolectin vs. Ensembl Zebrafish
Match: si:ch73-359m17.2 (si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-90])

HSP 1 Score: 46.2098 bits (108), Expect = 1.192e-6
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN      K  C+HT+++  PWW VDL G  ++  V I+NR +    RI GA
Sbjct:   50 GNTEADYRKGSCTHTSREFNPWWRVDLGGVSSVNKVTITNRGDCCEERIRGA 101
[Close]
BLAST of Fucolectin vs. Ensembl Xenopus
Match: ENSXETT00000033868.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:133949885:134039403:-1 gene:ENSXETG00000007052.1 transcript:ENSXETT00000033868.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.9214 bits (141), Expect = 6.959e-11
Identity = 24/46 (52.17%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            LNK  C+HTN+D QPWW +DL   Y + SVI+ NR +  S R++GA
Sbjct:  208 LNKSPCTHTNRDYQPWWQLDLDKRYQVKSVIVVNRRDCCSQRLLGA 253

HSP 2 Score: 45.0542 bits (105), Expect = 5.292e-6
Identity = 19/46 (41.30%), Postives = 29/46 (63.04%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            + K  C  TN++ +PWW +DL+  Y I +V+I NR +  S R+ GA
Sbjct:   60 IYKSPCVLTNRNHEPWWRLDLKKRYKIRTVVIVNRGDCCSERLKGA 105
[Close]
BLAST of Fucolectin vs. Ensembl Xenopus
Match: tpp1 (tripeptidyl peptidase I [Source:Xenbase;Acc:XB-GENE-997325])

HSP 1 Score: 56.9954 bits (136), Expect = 3.532e-10
Identity = 23/52 (44.23%), Postives = 36/52 (69.23%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            G  G ++ K+ C+HT +D QPWW +DLQ  Y + +V+I NR +  S+R++GA
Sbjct:   63 GIKGTNMGKNPCTHTQEDYQPWWRLDLQKRYKVETVVIVNRMDGSSSRLMGA 114

HSP 2 Score: 54.299 bits (129), Expect = 2.933e-9
Identity = 22/52 (42.31%), Postives = 35/52 (67.31%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            G  G ++ ++ CSHT +D QPWW +DLQ  Y + +V+I NR +  S+R++ A
Sbjct:  355 GIKGTNIMRNPCSHTQEDYQPWWRLDLQKRYKVETVVIVNRRDCCSSRLLAA 406

HSP 3 Score: 51.9878 bits (123), Expect = 2.297e-8
Identity = 31/99 (31.31%), Postives = 55/99 (55.56%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER-------DGAKGSKVRIRMT-RPEYL 344
            ++K  C+HT++D+  WW +DL+  Y + SV+I NR ++ S R++GA + V +  +D+  V    CG +    +       +G +G  V + +  R EYL
Sbjct:  509 IHKSPCTHTSQDNPAWWRLDLKKRYKVESVVIVNRMDEHSERLLGAEIRVGIFPVDNNPV----CGTITDVSQAAITLSCNGTEGRYVSVVIPGREEYL 603

HSP 4 Score: 49.6766 bits (117), Expect = 1.139e-7
Identity = 19/46 (41.30%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            ++K  C+HT++D+  WW +DL+  Y + SV+I NR ++ S R++GA
Sbjct:  217 IDKGPCTHTSQDNPAWWRLDLKKRYNVKSVVIVNRRDEHSERLLGA 262
[Close]
BLAST of Fucolectin vs. Ensembl Xenopus
Match: tpp1 (tripeptidyl peptidase I [Source:Xenbase;Acc:XB-GENE-997325])

HSP 1 Score: 54.299 bits (129), Expect = 3.346e-9
Identity = 21/49 (42.86%), Postives = 34/49 (69.39%), Query Frame = 3 
Query:   63 GQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            G ++ ++ CSHT +D QPWW +DLQ  Y + +V+I NR +  S+R++ A
Sbjct:  472 GTNIMRNPCSHTQEDYQPWWRLDLQKRYKVETVVIVNRRDCCSSRLLAA 520

HSP 2 Score: 52.373 bits (124), Expect = 1.435e-8
Identity = 21/46 (45.65%), Postives = 31/46 (67.39%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            L++  C+HTN + QPWW +DL+  Y + SVI+ NR +  S R+ GA
Sbjct:  183 LHQGPCTHTNNEYQPWWQLDLKKRYKVKSVIVVNRGDCCSERLKGA 228

HSP 3 Score: 51.9878 bits (123), Expect = 2.200e-8
Identity = 31/99 (31.31%), Postives = 55/99 (55.56%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER-------DGAKGSKVRIRMT-RPEYL 344
            ++K  C+HT++D+  WW +DL+  Y + SV+I NR ++ S R++GA + V +  +D+  V    CG +    +       +G +G  V + +  R EYL
Sbjct:  623 IHKSPCTHTSQDNPAWWRLDLKKRYKVESVVIVNRMDEHSERLLGAEIRVGIFPVDNNPV----CGTITDVSQAAITLSCNGTEGRYVSVVIPGREEYL 717

HSP 4 Score: 50.8322 bits (120), Expect = 5.695e-8
Identity = 20/52 (38.46%), Postives = 34/52 (65.38%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            G+   +++K  C+HTN D+ PWW + L+  Y I +V+I NR ++   R++GA
Sbjct:   29 GSKETNVSKYPCAHTNNDTAPWWQLQLEKRYKIETVVIVNRMDEHRERLLGA 80

HSP 5 Score: 48.521 bits (114), Expect = 3.428e-7
Identity = 19/46 (41.30%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            ++K  C+HT++D+  WW +DL+  Y + SV+I NR ++ S R+ GA
Sbjct:  331 IHKSPCTHTSQDNPAWWRLDLKKRYKVESVVIVNRMDEHSERLRGA 376
[Close]
BLAST of Fucolectin vs. Ensembl Xenopus
Match: ptx (pentraxin [Source:NCBI gene;Acc:100127855])

HSP 1 Score: 53.9138 bits (128), Expect = 5.212e-9
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKK 281
            G+L  +  +  CSHTNK  QPWW +D++G   + SV I+NR +    RI GA + +     +D  +   RCGVV K
Sbjct:  756 GSLSSNYMEGDCSHTNKQIQPWWQLDMRGKMRVHSVAITNRGDCCRERINGAEIRIGNSK-EDGGINNPRCGVVFK 830

HSP 2 Score: 50.8322 bits (120), Expect = 6.254e-8
Identity = 20/41 (48.78%), Postives = 29/41 (70.73%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNR 176
            G+L  +  + +CS+T K++ PWWMVDLQ  Y I SV ++NR
Sbjct:   64 GSLANNYLRSQCSYTKKETDPWWMVDLQKPYQILSVAVTNR 104

HSP 3 Score: 47.3654 bits (111), Expect = 8.022e-7
Identity = 21/41 (51.22%), Postives = 29/41 (70.73%), Query Frame = 3 
Query:   87 CSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            CSHT+ D +PWW VDL  +Y +T V I+NR +  ++RI GA
Sbjct:  429 CSHTDLDIEPWWRVDLTDTYNVTMVKITNRGDCCNDRINGA 469
[Close]
BLAST of Fucolectin vs. Ensembl Xenopus
Match: fucolectin (x-epilectin [Source:NCBI gene;Acc:100144955])

HSP 1 Score: 50.447 bits (119), Expect = 3.654e-8
Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   +     C+HTNKD  PWW +DL+ S  I +V+I+ R +  S R++GA
Sbjct:   66 GNKETNYYVHPCAHTNKDFAPWWQLDLKKSIKIWTVVITTRRDCCSERLLGA 117
[Close]
BLAST of Fucolectin vs. UniProt/SwissProt
Match: sp|Q9I931|FUCL1_ANGJA (Fucolectin-1 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 2.813e-7
Identity = 24/52 (46.15%), Postives = 27/52 (51.92%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN     +   CSHT     PWW VDL   Y ITSV I+NR +    RI GA
Sbjct:   60 GNRDSDFSHGSCSHTTNSPNPWWRVDLLQLYTITSVTITNRGDCCGERISGA 111
[Close]
BLAST of Fucolectin vs. UniProt/SwissProt
Match: sp|Q9I926|FUCL6_ANGJA (Fucolectin-6 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.546e-7
Identity = 23/52 (44.23%), Postives = 28/52 (53.85%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN         C+HT+  + PWW VDL   Y ITSV I+NR +    RI GA
Sbjct:   71 GNQDSYFYHGSCTHTSGGANPWWRVDLLQEYKITSVTITNRGDCCGERITGA 122
[Close]
BLAST of Fucolectin vs. UniProt/SwissProt
Match: sp|Q7SIC1|FUCL_ANGAN (Fucolectin OS=Anguilla anguilla OX=7936 PE=1 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 1.048e-6
Identity = 22/52 (42.31%), Postives = 29/52 (55.77%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   +     C+H++  + PWW VDL   Y ITSV I+NR +    RI GA
Sbjct:   39 GNRDSNFYHGSCTHSSGQANPWWRVDLLQVYTITSVTITNRGDCCGERISGA 90
[Close]
BLAST of Fucolectin vs. UniProt/SwissProt
Match: sp|Q9I925|FUCL7_ANGJA (Fucolectin-7 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.346e-6
Identity = 24/52 (46.15%), Postives = 28/52 (53.85%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN         C+HT  D +PWW VDL   Y ITSV I+NR +    RI GA
Sbjct:   71 GNRDSVFYHGSCTHTEAD-KPWWRVDLLQVYTITSVTITNRGDCCEERISGA 121
[Close]
BLAST of Fucolectin vs. UniProt/SwissProt
Match: sp|Q9I927|FUCL5_ANGJA (Fucolectin-5 OS=Anguilla japonica OX=7937 PE=2 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 3.634e-6
Identity = 21/42 (50.00%), Postives = 23/42 (54.76%), Query Frame = 3 
Query:   84 KCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
             CSHT     PWW VDL   Y I SV I+NR +    RI GA
Sbjct:   81 SCSHTTNGPNPWWRVDLLQVYTIASVTITNRGDCCGERITGA 122
[Close]
BLAST of Fucolectin vs. TrEMBL
Match: A0A0P7T675 (FTP domain-containing protein (Fragment) OS=Scleropages formosus OX=113540 GN=Z043_125975 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.480e-9
Identity = 35/99 (35.35%), Postives = 51/99 (51.52%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVV--------KKDERDGAKGSKVRIRMTRPEYL 344
             +   C+HT +DS+PWW VDL   YA+TSV I+NR +  + RI GA    I   LDD       C V+        +  + +G  G  V + + RP++L
Sbjct:   33 FSDHSCTHTERDSKPWWKVDLHNVYAVTSVTITNRGDCCAQRINGA-EIRIGNSLDDNGNQNPLCAVITSIPAGNSRTFQCNGMTGRYVNVVLPRPDFL 130
[Close]
BLAST of Fucolectin vs. TrEMBL
Match: A0A5G3HRL1 (Uncharacterized protein OS=Xenopus tropicalis OX=8364 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.093e-7
Identity = 24/46 (52.17%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            LNK  C+HTN+D QPWW +DL   Y + SVI+ NR +  S R++GA
Sbjct:  208 LNKSPCTHTNRDYQPWWQLDLDKRYQVKSVIVVNRRDCCSQRLLGA 253
[Close]
BLAST of Fucolectin vs. TrEMBL
Match: A0A3Q3L4K6 (Uncharacterized protein OS=Labrus bergylta OX=56723 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.350e-7
Identity = 30/74 (40.54%), Postives = 44/74 (59.46%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVV 275
            GN   +  K  CS T+++S PWW VDLQ  Y +T+V+I+NR N+  NR+ GA    I   L+D     +RC ++
Sbjct:  227 GNRDPNFAKGSCSLTHQESNPWWRVDLQNVYTVTAVMITNR-NEFENRLDGA-EIWIGNSLEDNGAKKSRCAII 298
[Close]
BLAST of Fucolectin vs. TrEMBL
Match: A0A1L8FTZ7 (Uncharacterized protein OS=Xenopus laevis OX=8355 GN=fucolectinloc100491211 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.358e-7
Identity = 36/99 (36.36%), Postives = 54/99 (54.55%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER-------DGAKGSKVRIRMT-RPEYL 344
            L++  C+HTN+D QPWW +DL+  Y + SVII NR +  S R+ GA + V     ++ HV    CG +K   +       +G +G  V + +  R EYL
Sbjct:  349 LHEGPCTHTNQDYQPWWQLDLKKRYKVKSVIIVNRGDCCSERLKGAEIRVGDSSENNNHV----CGTIKDVSQTTNILFCNGMEGRYVSVVIPGRKEYL 443

HSP 2 Score: 55.0694 bits (131), Expect = 2.625e-6
Identity = 36/99 (36.36%), Postives = 52/99 (52.53%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER-------DGAKGSKVRIRMT-RPEYL 344
            L+K  CSHTNKD+  WW +DL+  Y + SV+I NR +  S+R+ GA + V    +DD  V    CG +            +G +G  V + +  R EYL
Sbjct:  645 LHKSPCSHTNKDNPAWWRLDLKKRYNVQSVVIVNRMDGHSDRLRGATIRVGNFAVDDNPV----CGTIHDVSEATITLPCNGMEGRYVSVVIPGRAEYL 739

HSP 3 Score: 55.0694 bits (131), Expect = 2.703e-6
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:   72 LNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            L++  C+HTN+D QPWW +DL+  Y + SVII NR +  S R+ GA
Sbjct:  497 LHQGPCTHTNQDYQPWWQLDLKKRYKVKSVIIVNRGDCCSERLKGA 542
[Close]
BLAST of Fucolectin vs. TrEMBL
Match: A0A3Q3FVM4 (Uncharacterized protein OS=Labrus bergylta OX=56723 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.772e-7
Identity = 30/74 (40.54%), Postives = 44/74 (59.46%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVV 275
            GN   +  K  CS T+++S PWW VDLQ  Y +T+V+I+NR N+  NR+ GA    I   L+D     +RC ++
Sbjct:   79 GNRDPNFAKGSCSLTHQESNPWWRVDLQNVYTVTAVMITNR-NEFENRLDGA-EIWIGNSLEDNGAKKSRCAII 150
[Close]
BLAST of Fucolectin vs. Ensembl Cavefish
Match: ENSAMXT00000008082.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000995.1:63101:75869:-1 gene:ENSAMXG00000007863.2 transcript:ENSAMXT00000008082.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 4.891e-10
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   + N   C+HT  +  PWW +DL+G Y ITS+ I+NR +  S RI GA
Sbjct:  197 GNYFSNANYGSCTHTATEENPWWRLDLEGEYEITSITITNRGDCCSERIDGA 248

HSP 2 Score: 51.6026 bits (122), Expect = 1.263e-8
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   +     C+HT  +  PWW +DLQ  Y ITS+ I+NR +  S RI GA
Sbjct:   49 GNYFGTTTYGSCTHTATEQNPWWRLDLQDEYKITSITITNRGDCCSERINGA 100
[Close]
BLAST of Fucolectin vs. Ensembl Cavefish
Match: ENSAMXT00000037268.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000995.1:63101:73310:-1 gene:ENSAMXG00000007863.2 transcript:ENSAMXT00000037268.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 4.987e-10
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   + N   C+HT  +  PWW +DL+G Y ITS+ I+NR +  S RI GA
Sbjct:  207 GNYFSNANYGSCTHTATEENPWWRLDLEGEYEITSITITNRGDCCSERIDGA 258

HSP 2 Score: 51.9878 bits (123), Expect = 1.255e-8
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   +     C+HT  +  PWW +DLQ  Y ITS+ I+NR +  S RI GA
Sbjct:   59 GNYFGTTTYGSCTHTATEQNPWWRLDLQDEYKITSITITNRGDCCSERINGA 110
[Close]
BLAST of Fucolectin vs. Ensembl Cavefish
Match: ENSAMXT00000056118.1 (fucolectin-4-like [Source:NCBI gene;Acc:103037148])

HSP 1 Score: 50.8322 bits (120), Expect = 1.093e-8
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   + +   CSHT+  + PWW VDL   Y ITS+ I+NR +  +NRI GA
Sbjct:   52 GNPNSNYHDGSCSHTSTQNNPWWRVDLLYKYKITSITITNRGDCCANRINGA 103
[Close]
BLAST of Fucolectin vs. Ensembl Cavefish
Match: si:ch73-359m17.2 (si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-90])

HSP 1 Score: 50.8322 bits (120), Expect = 3.017e-8
Identity = 22/52 (42.31%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN      +  C+HT  +  PWW V+L G Y +TSV I+NR +    RI+GA
Sbjct:  196 GNSDSHYFRGSCTHTMHEKDPWWRVELPGVYLVTSVTITNRGDCCGFRIIGA 247

HSP 2 Score: 44.669 bits (104), Expect = 3.562e-6
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 3 
Query:   87 CSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            CSHTN +  PW  V+L   Y ITSV I+NR +    RI GA
Sbjct:   59 CSHTNYEKNPWLRVELPSIYNITSVTITNRGDCCGQRIHGA 99
[Close]
BLAST of Fucolectin vs. Ensembl Cavefish
Match: ENSAMXT00000058044.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000247.1:335638:339087:-1 gene:ENSAMXG00000031034.1 transcript:ENSAMXT00000058044.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 5.078e-8
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGA 209
            GN   +  +  CSHT     PWW VDL   Y ITS+ I+NR+  +S R+ GA
Sbjct:   56 GNTDANFERGSCSHTASQQNPWWRVDLLEEYTITSITITNRDY-LSERLNGA 106
[Close]
BLAST of Fucolectin vs. Ensembl Nematostella
Match: EDO35302 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLU7])

HSP 1 Score: 43.8986 bits (102), Expect = 2.681e-6
Identity = 18/51 (35.29%), Postives = 30/51 (58.82%), Query Frame = 3 
Query:   54 GNLGQSLNKDK-CSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIV 203
            GN     N+ + C+HT+  + PWW VDL  +  ++ V + NR  D++NR+ 
Sbjct:    7 GNTDGDFNRGRSCTHTDAHTNPWWRVDLGTTQPVSEVFLVNRLGDLANRLF 57
[Close]
BLAST of Fucolectin vs. Ensembl Nematostella
Match: EDO46933 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNJ3])

HSP 1 Score: 43.5134 bits (101), Expect = 4.516e-6
Identity = 17/45 (37.78%), Postives = 25/45 (55.56%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDM 188
            G++  SL++  C  T  ++ PWW VDLQ  Y +T V I+     M
Sbjct: 1141 GDMSSSLSEGSCIATQSETDPWWRVDLQREYIVTDVSIAEEERSM 1185
[Close]
BLAST of Fucolectin vs. Planmine SMEST
Match: SMESG000014428.1 (SMESG000014428.1)

HSP 1 Score: 135.576 bits (340), Expect = 5.984e-42
Identity = 74/103 (71.84%), Postives = 83/103 (80.58%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER------DGAKGSKVRIRMTRPEYL 344
            GN+GQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVII NRN+ +SNR+VGA    IVEVLD   +VV+RCG VK D+         ++GSKVRIRMTRPEYL
Sbjct:   43 GNVGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIIFNRNSFVSNRLVGA----IVEVLDSNGLVVSRCGEVKTDDSVHLLHCQKSRGSKVRIRMTRPEYL 141
[Close]
BLAST of Fucolectin vs. Planmine SMEST
Match: SMESG000014445.1 (SMESG000014445.1)

HSP 1 Score: 128.642 bits (322), Expect = 2.929e-39
Identity = 72/103 (69.90%), Postives = 78/103 (75.73%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKDER------DGAKGSKVRIRMTRPEYL 344
            GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSY ITSV I NRN+ +SNR+VGA    IVEVLD   VVV+RCG +K D+           GSKVRIRM RPEYL
Sbjct:   43 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYTITSVTIFNRNSFVSNRLVGA----IVEVLDSNGVVVSRCGKIKTDDSVHILRCKKRIGSKVRIRMARPEYL 141
[Close]
BLAST of Fucolectin vs. Planmine SMEST
Match: SMESG000014425.1 (SMESG000014425.1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.513e-20
Identity = 50/106 (47.17%), Postives = 63/106 (59.43%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCG---------VVKKDERDGAKGSKVRIRMTRPEYL 344
            GN GQ+L KD CS T+ D +PWWMVDL+    I  VI+ NR +   NRIVGA    I+++LD ++  V  CG         VV  +E  G  GSKVRIR+ R EYL
Sbjct:   43 GNKGQNLLKDGCSQTSNDYRPWWMVDLENEIQIGRVIVFNRVDAYQNRIVGA----IIDILDKENKFVGTCGMINAYDPISVVWCNESHGMLGSKVRIRIERQEYL 144
[Close]
BLAST of Fucolectin vs. Planmine SMEST
Match: SMESG000014421.1 (SMESG000014421.1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.139e-20
Identity = 49/106 (46.23%), Postives = 66/106 (62.26%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKK---------DERDGAKGSKVRIRMTRPEYL 344
            GN GQ+L KD CS T+ +++PWWMV+L+    I+ V+I NR +D  +RIVGA    IV+VLD ++  V  CG++ K         +   G  GSKVRIRM R EYL
Sbjct:   64 GNHGQNLLKDGCSQTSNENRPWWMVNLENEIRISMVMIFNRVDDYQDRIVGA----IVDVLDKENKFVGTCGIITKYDPISLMWCNNGTGILGSKVRIRMERQEYL 165
[Close]
BLAST of Fucolectin vs. Planmine SMEST
Match: SMESG000014441.1 (SMESG000014441.1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.713e-19
Identity = 49/106 (46.23%), Postives = 60/106 (56.60%), Query Frame = 3 
Query:   54 GNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAITSVIISNRNNDMSNRIVGAXXXXXXXXLDDKHVVVNRCGVVKKD---------ERDGAKGSKVRIRMTRPEYL 344
            GN GQ L K KCS T+ D++PWWMV+L     IT V I NR +  S RI GA    +VEVLD    V   CG+V  D         +R G  GSKVR+++ R EYL
Sbjct:   43 GNNGQHLEKHKCSQTSNDNRPWWMVNLTVEAKITLVTIFNRQDQFSERIEGA----VVEVLDKNDEVTGLCGIVMADNSVINLWCNQRAGIFGSKVRVKLERQEYL 144
The following BLAST results are available for this feature:
BLAST of Fucolectin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch73-359m17.25.278e-740.38si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-... [more]
si:cabz01040626.26.380e-748.78si:cabz01040626.2 [Source:ZFIN;Acc:ZDB-GENE-160113... [more]
si:cabz01040626.27.041e-748.78si:cabz01040626.2 [Source:ZFIN;Acc:ZDB-GENE-160113... [more]
si:ch73-359m17.21.086e-640.38si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-... [more]
si:ch73-359m17.21.192e-640.38si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-... [more]
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BLAST of Fucolectin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000033868.16.959e-1152.17pep primary_assembly:Xenopus_tropicalis_v9.1:6:133... [more]
tpp13.532e-1044.23tripeptidyl peptidase I [Source:Xenbase;Acc:XB-GEN... [more]
tpp13.346e-942.86tripeptidyl peptidase I [Source:Xenbase;Acc:XB-GEN... [more]
ptx5.212e-939.47pentraxin [Source:NCBI gene;Acc:100127855][more]
fucolectin3.654e-842.31x-epilectin [Source:NCBI gene;Acc:100144955][more]
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BLAST of Fucolectin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9I931|FUCL1_ANGJA2.813e-746.15Fucolectin-1 OS=Anguilla japonica OX=7937 PE=2 SV=... [more]
sp|Q9I926|FUCL6_ANGJA3.546e-744.23Fucolectin-6 OS=Anguilla japonica OX=7937 PE=2 SV=... [more]
sp|Q7SIC1|FUCL_ANGAN1.048e-642.31Fucolectin OS=Anguilla anguilla OX=7936 PE=1 SV=1[more]
sp|Q9I925|FUCL7_ANGJA2.346e-646.15Fucolectin-7 OS=Anguilla japonica OX=7937 PE=2 SV=... [more]
sp|Q9I927|FUCL5_ANGJA3.634e-650.00Fucolectin-5 OS=Anguilla japonica OX=7937 PE=2 SV=... [more]
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BLAST of Fucolectin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0P7T6756.480e-935.35FTP domain-containing protein (Fragment) OS=Sclero... [more]
A0A5G3HRL11.093e-752.17Uncharacterized protein OS=Xenopus tropicalis OX=8... [more]
A0A3Q3L4K61.350e-740.54Uncharacterized protein OS=Labrus bergylta OX=5672... [more]
A0A1L8FTZ71.358e-736.36Uncharacterized protein OS=Xenopus laevis OX=8355 ... [more]
A0A3Q3FVM41.772e-740.54Uncharacterized protein OS=Labrus bergylta OX=5672... [more]
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BLAST of Fucolectin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000008082.24.891e-1046.15pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000037268.14.987e-1046.15pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000056118.11.093e-846.15fucolectin-4-like [Source:NCBI gene;Acc:103037148][more]
si:ch73-359m17.23.017e-842.31si:ch73-359m17.2 [Source:ZFIN;Acc:ZDB-GENE-141222-... [more]
ENSAMXT00000058044.15.078e-844.23pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Fucolectin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO353022.681e-635.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO469334.516e-637.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Fucolectin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fucolectin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014428.15.984e-4271.84SMESG000014428.1[more]
SMESG000014445.12.929e-3969.90SMESG000014445.1[more]
SMESG000014425.12.513e-2047.17SMESG000014425.1[more]
SMESG000014421.14.139e-2046.23SMESG000014421.1[more]
SMESG000014441.11.713e-1946.23SMESG000014441.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032543 ID=SMED30032543|Name=Fucolectin|organism=Schmidtea mediterranea sexual|type=transcript|length=344bp
CAGCCTCTCAATCAAGTGATTATTCCTCCTATGTAGCTAGCTATGCTGTC
GATGGAAATCTTGGCCAATCTCTTAATAAGGATAAATGTAGCCACACAAA
TAAAGACAGCCAACCTTGGTGGATGGTAGATTTACAAGGTTCTTACGCAA
TAACTTCTGTGATTATATCCAACCGAAATAACGACATGAGTAACAGAATA
GTTGGGGCGATAGTTGAGGTGATAGTTGAAGTGTTGGATGATAAACATGT
AGTGGTTAACAGATGTGGAGTGGTGAAAAAAGATGAAAGGGATGGTGCAA
AAGGATCTAAAGTAAGAATAAGAATGACGAGGCCAGAATATCTG
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protein sequence of SMED30032543-orf-1

>SMED30032543-orf-1 ID=SMED30032543-orf-1|Name=SMED30032543-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp
ASQSSDYSSYVASYAVDGNLGQSLNKDKCSHTNKDSQPWWMVDLQGSYAI
TSVIISNRNNDMSNRIVGAIVEVIVEVLDDKHVVVNRCGVVKKDERDGAK
GSKVRIRMTRPEYL
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR006585FTP1
IPR008979Galactose-bd-like_sf
IPR000421FA58C
Vocabulary: biological process
TermDefinition
GO:0001868regulation of complement activation, lectin pathway
GO:0010185regulation of cellular defense response
GO:0045088regulation of innate immune response
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0030246carbohydrate binding
GO:0042806fucose binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
GO:0005615extracellular space
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008979Galactose-binding-like domain superfamilyGENE3DG3DSA:2.60.120.260coord: 1..72
e-value: 4.7E-6
score: 28.5