SMED30032529

Overview


Name	SMED30032529
Smed ID	SMED30032529
Length (bp)	1253

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032529 (SMED30032529) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032529 (SMED30032529) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032529

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30032529	SMESG000077536.1	SmedASXL_015030	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30032529	SMESG000077536.1	dd_Smed_v4_11079_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30032529	SMESG000077536.1	dd_Smed_v4_11079_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30032529 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.007787:18069..19464 -	5580

Homology

[Close]

BLAST of SMED30032529 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 56.6102 bits (135), Expect = 6.545e-8
Identity = 40/114 (35.09%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 56.6102 bits (135), Expect = 6.851e-8
Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDC 334

[Close]

BLAST of SMED30032529 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 55.8398 bits (133), Expect = 6.933e-8
Identity = 40/114 (35.09%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 56.225 bits (134), Expect = 7.212e-8
Identity = 40/114 (35.09%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 56.225 bits (134), Expect = 7.623e-8
Identity = 40/114 (35.09%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:   22 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 130

[Close]

BLAST of SMED30032529 vs. Ensembl Fly
Match: Kank (gene:FBgn0027596 transcript:FBtr0303758)

HSP 1 Score: 51.9878 bits (123), Expect = 6.256e-7
Identity = 38/125 (30.40%), Postives = 65/125 (52.00%), Query Frame = 2

Query:  299 KSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI-LNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFKYE 670
            +SLS  LLEY   ++  D  G  A+H++V     D+V I L+S   D+N  NN G+T++     ++ K     +++ ++ K    +NI   K   + LM ++ H N   V +L+D GA  + + E
Sbjct:  236 ESLSIPLLEYC--VNLSDSNGNTAMHYAVSHGNFDVVSILLDSKVCDVNQMNNAGYTSVMLVSLAKLKQPAHRTVVERLFKMA-DVNIRAKKHCQTALMLAVSHGNGDMVNMLLDAGADINIQDE 357

[Close]

BLAST of SMED30032529 vs. Ensembl Fly
Match: Kank (gene:FBgn0027596 transcript:FBtr0303756)

HSP 1 Score: 52.373 bits (124), Expect = 6.763e-7
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 2

Query:  302 SLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI-LNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            SLS  LLEY   ++  D  G  A+H++V     D+V I L+S   D+N  NN G+T++     ++ K     +++ ++ K    +NI   K   + LM ++ H N   V +L+D GA
Sbjct:  854 SLSIPLLEYC--VNLSDSNGNTAMHYAVSHGNFDVVSILLDSKVCDVNQMNNAGYTSVMLVSLAKLKQPAHRTVVERLFKM-ADVNIRAKKHCQTALMLAVSHGNGDMVNMLLDAGA 967

[Close]

BLAST of SMED30032529 vs. Ensembl Fly
Match: Kank (gene:FBgn0027596 transcript:FBtr0087431)

HSP 1 Score: 51.9878 bits (123), Expect = 7.805e-7
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 2

Query:  302 SLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI-LNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            SLS  LLEY   ++  D  G  A+H++V     D+V I L+S   D+N  NN G+T++     ++ K     +++ ++ K    +NI   K   + LM ++ H N   V +L+D GA
Sbjct:  833 SLSIPLLEYC--VNLSDSNGNTAMHYAVSHGNFDVVSILLDSKVCDVNQMNNAGYTSVMLVSLAKLKQPAHRTVVERLFKM-ADVNIRAKKHCQTALMLAVSHGNGDMVNMLLDAGA 946

[Close]

BLAST of SMED30032529 vs. Ensembl Fly
Match: Kank (gene:FBgn0027596 transcript:FBtr0332856)

HSP 1 Score: 51.9878 bits (123), Expect = 8.146e-7
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 2

Query:  302 SLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI-LNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            SLS  LLEY   ++  D  G  A+H++V     D+V I L+S   D+N  NN G+T++     ++ K     +++ ++ K    +NI   K   + LM ++ H N   V +L+D GA
Sbjct:  830 SLSIPLLEYC--VNLSDSNGNTAMHYAVSHGNFDVVSILLDSKVCDVNQMNNAGYTSVMLVSLAKLKQPAHRTVVERLFKM-ADVNIRAKKHCQTALMLAVSHGNGDMVNMLLDAGA 943

[Close]

BLAST of SMED30032529 vs. Ensembl Fly
Match: Kank (gene:FBgn0027596 transcript:FBtr0087430)

HSP 1 Score: 51.9878 bits (123), Expect = 9.751e-7
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 2

Query:  302 SLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI-LNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            SLS  LLEY   ++  D  G  A+H++V     D+V I L+S   D+N  NN G+T++     ++ K     +++ ++ K    +NI   K   + LM ++ H N   V +L+D GA
Sbjct: 1015 SLSIPLLEYC--VNLSDSNGNTAMHYAVSHGNFDVVSILLDSKVCDVNQMNNAGYTSVMLVSLAKLKQPAHRTVVERLFKM-ADVNIRAKKHCQTALMLAVSHGNGDMVNMLLDAGA 1128

[Close]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Match: CABZ01071939.1 (pep chromosome:GRCz11:19:2018796:2029777:-1 gene:ENSDARG00000086326.3 transcript:ENSDART00000128639.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01071939.1)

HSP 1 Score: 51.9878 bits (123), Expect = 9.475e-7
Identity = 37/118 (31.36%), Postives = 57/118 (48.31%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFKYE 670
            LL  G  I++ + YG  ALH +   N +D+V   L +   D+N  N  GF+ALH+   S      LE LI      + +LN  +K   +PL  +  H  +   + +I NGA  + + E
Sbjct:   71 LLSLGVDINEPNAYGNTALHLACF-NGQDVVVNELIAAGADVNQVNEKGFSALHFTAASRQGALCLELLIAN----RANLNCKSKDGKTPLHMAAIHGRYSRSQAIIQNGAEINCEDE 183

[Close]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Match: ankrd44 (ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE-070912-605])

HSP 1 Score: 51.9878 bits (123), Expect = 1.017e-6
Identity = 35/115 (30.43%), Postives = 53/115 (46.09%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL    +ID+ + +G  ALH +   N +D V   L  +  +++  NN GFT LH+A  S      LE L+         +N+ ++   SPL  +  H  F   + LI NG   D 
Sbjct:  216 LLSLAVEIDEANAFGNTALHVACF-NGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLV----NSGADVNVQSRDGKSPLHLTAVHGRFTRSQTLIQNGGEIDC 325

[Close]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Match: ankrd44 (ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE-070912-605])

HSP 1 Score: 51.9878 bits (123), Expect = 1.060e-6
Identity = 35/115 (30.43%), Postives = 53/115 (46.09%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL    +ID+ + +G  ALH +   N +D V   L  +  +++  NN GFT LH+A  S      LE L+         +N+ ++   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLSLAVEIDEANAFGNTALHVACF-NGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLV----NSGADVNVQSRDGKSPLHLTAVHGRFTRSQTLIQNGGEIDC 334

[Close]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Match: rai14 (retinoic acid induced 14 [Source:ZFIN;Acc:ZDB-GENE-040718-235])

HSP 1 Score: 51.6026 bits (122), Expect = 1.493e-6
Identity = 42/159 (26.42%), Postives = 73/159 (45.91%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            A+  G   +V  LL+K+     K+ S+    L      T         +L +GA +  +D  GF ALH +   N  +  +  L +    ++  ++ G TALH+A    +  EI++ L     + KY +N+ +    +PL+ S RH +    + L+D GA
Sbjct:   26 AVEHGEADKVTSLLSKKGASPTKLDSEGKSAL--HAAATRGQTECLAVILAHGADVSLQDASGFTALHLAAKNNHPECAKKLLQNKCV-VDAPDSSGRTALHHAAACGNN-EIVQLLC----ENKYHVNLKDSDGFTPLLLSARHAHAEVCQSLLDWGA 176

[Close]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Match: rai14 (retinoic acid induced 14 [Source:ZFIN;Acc:ZDB-GENE-040718-235])

HSP 1 Score: 51.6026 bits (122), Expect = 1.527e-6
Identity = 42/159 (26.42%), Postives = 73/159 (45.91%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            A+  G   +V  LL+K+     K+ S+    L      T         +L +GA +  +D  GF ALH +   N  +  +  L +    ++  ++ G TALH+A    +  EI++ L     + KY +N+ +    +PL+ S RH +    + L+D GA
Sbjct:   26 AVEHGEADKVTSLLSKKGASPTKLDSEGKSAL--HAAATRGQTECLAVILAHGADVSLQDASGFTALHLAAKNNHPECAKKLLQNKCV-VDAPDSSGRTALHHAAACGNN-EIVQLLC----ENKYHVNLKDSDGFTPLLLSARHAHAEVCQSLLDWGA 176

[Close]

BLAST of SMED30032529 vs. Ensembl Xenopus
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:549110])

HSP 1 Score: 55.4546 bits (132), Expect = 3.345e-8
Identity = 36/133 (27.07%), Postives = 59/133 (44.36%), Query Frame = 2

Query:  281 TDTFWAKSLSFT-----LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            T   WA S   T     LL  G  +D KD+ G+  LH +    + D+V   +      +N  N  G T LHYA  S++K EI       + +   S +  +   ++PL  +    N   V++L+ + A T+ +
Sbjct:   43 TALHWACSAGRTEVAEYLLRLGVPVDAKDDAGWTPLHIAASAGRDDIVRALIGKG-AQVNAANQIGCTPLHYAA-SKNKHEI----ALMLLENGASPDAKDNLESTPLHRAASKGNLRIVQILLKHQASTNIQ 169

[Close]

BLAST of SMED30032529 vs. Ensembl Xenopus
Match: ankrd52 (ankyrin repeat domain 52 [Source:NCBI gene;Acc:100127696])

HSP 1 Score: 51.2174 bits (121), Expect = 2.629e-6
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL+ G +ID+ + +G  ALH +    + D V   L ++  ++N  N  GFT LH+A  S +    LE L+         +N  +K   SPL  +  H  F   ++LI NG   D
Sbjct:  229 LLKLGVEIDEPNAFGNTALHIACYMGQ-DAVANELVNYGSNVNQPNEKGFTPLHFAAVSTNGALCLELLV----NNGADVNFQSKEGKSPLHMAAIHGRFTRSQILIQNGGEID 337

[Close]

BLAST of SMED30032529 vs. Ensembl Xenopus
Match: ankrd52 (ankyrin repeat domain 52 [Source:NCBI gene;Acc:100127696])

HSP 1 Score: 51.2174 bits (121), Expect = 2.647e-6
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL+ G +ID+ + +G  ALH +    + D V   L ++  ++N  N  GFT LH+A  S +    LE L+         +N  +K   SPL  +  H  F   ++LI NG   D
Sbjct:  225 LLKLGVEIDEPNAFGNTALHIACYMGQ-DAVANELVNYGSNVNQPNEKGFTPLHFAAVSTNGALCLELLV----NNGADVNFQSKEGKSPLHMAAIHGRFTRSQILIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Xenopus
Match: ankrd52 (ankyrin repeat domain 52 [Source:NCBI gene;Acc:100127696])

HSP 1 Score: 51.2174 bits (121), Expect = 3.017e-6
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL+ G +ID+ + +G  ALH +    + D V   L ++  ++N  N  GFT LH+A  S +    LE L+         +N  +K   SPL  +  H  F   ++LI NG   D
Sbjct:  225 LLKLGVEIDEPNAFGNTALHIACYMGQ-DAVANELVNYGSNVNQPNEKGFTPLHFAAVSTNGALCLELLV----NNGADVNFQSKEGKSPLHMAAIHGRFTRSQILIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Xenopus
Match: ankrd52 (ankyrin repeat domain 52 [Source:NCBI gene;Acc:100127696])

HSP 1 Score: 51.2174 bits (121), Expect = 3.078e-6
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL+ G +ID+ + +G  ALH +    + D V   L ++  ++N  N  GFT LH+A  S +    LE L+         +N  +K   SPL  +  H  F   ++LI NG   D
Sbjct:  225 LLKLGVEIDEPNAFGNTALHIACYMGQ-DAVANELVNYGSNVNQPNEKGFTPLHFAAVSTNGALCLELLV----NNGADVNFQSKEGKSPLHMAAIHGRFTRSQILIQNGGEID 333

[Close]

BLAST of SMED30032529 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 56.9954 bits (136), Expect = 3.178e-8
Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDC 334

[Close]

BLAST of SMED30032529 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 56.9954 bits (136), Expect = 3.364e-8
Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDC 334

[Close]

BLAST of SMED30032529 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 56.6102 bits (135), Expect = 4.315e-8
Identity = 40/114 (35.09%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:   22 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 130

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BLAST of SMED30032529 vs. Ensembl Mouse
Match: Ankrd52 (ankyrin repeat domain 52 [Source:MGI Symbol;Acc:MGI:2444029])

HSP 1 Score: 54.6842 bits (130), Expect = 1.636e-7
Identity = 38/114 (33.33%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  GA+ID+ + +G  ALH +    + D V I L +   ++N  N+ GFT LH A  S +    LE L+       Y     +K   SPL  +  H  F   ++LI NG+  D
Sbjct:  225 LLRMGAEIDEPNAFGNTALHIACYLGQ-DAVAIELVNAGANVNQPNDKGFTPLHVAAVSTNGALCLELLVNNGADVNYQ----SKEGKSPLHMAAIHGRFTRSQILIQNGSEID 333

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BLAST of SMED30032529 vs. Ensembl Mouse
Match: A26c3 (ANKRD26-like family C, member 3 [Source:MGI Symbol;Acc:MGI:1914825])

HSP 1 Score: 50.0618 bits (118), Expect = 3.385e-6
Identity = 46/165 (27.88%), Postives = 82/165 (49.70%), Query Frame = 2

Query:  293 WAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNG---------ARTDFKY--EGLS------FLDYFNNLKRKLKET-PEY 733
            W       LL++GA  ++KD+ G  +LH++V ++ + L +  L  +  ++  KN  GFT L  A+  E+KIE+ + L+    K   ++++ +    + L+ +IR  +   V +L+D G           T  +Y  EG S       ++Y   L+RK K+  PEY
Sbjct:  123 WTYECVCILLKHGADPNRKDKNGNTSLHYAVSEDNQTLAKCLL-KYSANMEQKNKDGFTPLLLAL-KENKIEMAKFLV----KMGANIHVFDDMRRNTLLYAIRWDSKDMVNLLLDEGIDFFFRDVFGWTALRYAIEGTSKGSREILMNYDEMLRRKNKDGIPEY 281

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BLAST of SMED30032529 vs. UniProt/SwissProt
Match: sp|B2RXR6|ANR44_MOUSE (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Mus musculus OX=10090 GN=Ankrd44 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.223e-7
Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDC 334

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BLAST of SMED30032529 vs. UniProt/SwissProt
Match: sp|Q8N8A2|ANR44_HUMAN (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Homo sapiens OX=9606 GN=ANKRD44 PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 3.290e-7
Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  G +ID+ + YG  ALH +   N +D V   L  +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D 
Sbjct:  225 LLNLGVEIDEINVYGNTALHIACY-NGQDAVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDC 334

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BLAST of SMED30032529 vs. UniProt/SwissProt
Match: sp|Q5F478|ANR44_CHICK (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Gallus gallus OX=9031 GN=ANKRD44 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.499e-7
Identity = 37/114 (32.46%), Postives = 54/114 (47.37%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  G +ID+ + YG  ALH +    +  +V   ++ +  ++N  NN GFT LH+A  S      LE L+         +NI +K   SPL  +  H  F   + LI NG   D
Sbjct:  225 LLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELID-YGANVNQPNNNGFTPLHFAAASTHGALCLELLV----NNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEID 333

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BLAST of SMED30032529 vs. UniProt/SwissProt
Match: sp|Q4UMH6|Y381_RICFE (Putative ankyrin repeat protein RF_0381 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=RF_0381 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 6.080e-7
Identity = 43/162 (26.54%), Postives = 73/162 (45.06%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            A+  G    V+ L+  +A+   K  +   ++       +F    L + L+ YGA ++ K + G  ALH++V     DLV + + S   D+N K N G T L+ AV+  S       L++ +  Y   +N       + L  ++   N   V +LI NGA  +
Sbjct:  678 AVKSGNLHLVKWLIENQANIHAKTDNGETVL---HYAVSFNNSDLVYLLIAYGADVNAKTDNGLTALHYAVYDGNLDLVSLLI-SHGADVNAKTNSGETILYSAVDYGS-----PDLVYLLIAYGADVNAKTDNGETVLHYAVESGNLDLVSLLIHNGANVN 830

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BLAST of SMED30032529 vs. UniProt/SwissProt
Match: sp|Q8BTI7|ANR52_MOUSE (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C OS=Mus musculus OX=10090 GN=Ankrd52 PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.145e-6
Identity = 38/114 (33.33%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  GA+ID+ + +G  ALH +    + D V I L +   ++N  N+ GFT LH A  S +    LE L+       Y     +K   SPL  +  H  F   ++LI NG+  D
Sbjct:  225 LLRMGAEIDEPNAFGNTALHIACYLGQ-DAVAIELVNAGANVNQPNDKGFTPLHVAAVSTNGALCLELLVNNGADVNYQ----SKEGKSPLHMAAIHGRFTRSQILIQNGSEID 333

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BLAST of SMED30032529 vs. TrEMBL
Match: A0A267DSQ1 (ANK_REP_REGION domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020439g2 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 4.750e-22
Identity = 53/151 (35.10%), Postives = 90/151 (59.60%), Query Frame = 2

Query:  155 ASSPAEAIYRGLFKQVRELLN----KEADPDEKIKS-KTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLM 592
            ++SPAEAI  G F QVR LL       AD  E +   +T ++LC+ + D  W+ SL+  LLE GAK+  KD  G NALH++    +++L E+FL + D+ ++  + +  +A+HYAV    ++ I+E+L   +++ +  ++  N+   +PLM
Sbjct:   56 SASPAEAILAGYFAQVRYLLQTGGRGAADTREPLAGGRTPLILCSLVRDRCWSVSLARLLLEKGAKVGLKDRRGMNALHYACAHGREELAELFLQAIDYRVDAADGYRNSAMHYAVRG-GRLRIVEALAKVLQRLQLPVSPPNREGVTPLM 205

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BLAST of SMED30032529 vs. TrEMBL
Match: A0A267E3L7 (ANK_REP_REGION domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020439g1 PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 5.222e-22
Identity = 53/151 (35.10%), Postives = 90/151 (59.60%), Query Frame = 2

Query:  155 ASSPAEAIYRGLFKQVRELLN----KEADPDEKIKS-KTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLM 592
            ++SPAEAI  G F QVR LL       AD  E +   +T ++LC+ + D  W+ SL+  LLE GAK+  KD  G NALH++    +++L E+FL + D+ ++  + +  +A+HYAV    ++ I+E+L   +++ +  ++  N+   +PLM
Sbjct:   63 SASPAEAILAGYFAQVRYLLQTGGRGAADTREPLAGGRTPLILCSLVRDRCWSVSLARLLLEKGAKVGLKDRRGMNALHYACAHGREELAELFLQAIDYRVDAADGYRNSAMHYAVRG-GRLRIVEALAKVLQRLQLPVSPPNREGVTPLM 212

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BLAST of SMED30032529 vs. TrEMBL
Match: A0A1I8H035 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 5.726e-22
Identity = 53/151 (35.10%), Postives = 90/151 (59.60%), Query Frame = 2

Query:  155 ASSPAEAIYRGLFKQVRELLN----KEADPDEKIKS-KTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLM 592
            ++SPAEAI  G F QVR LL       AD  E +   +T ++LC+ + D  W+ SL+  LLE GAK+  KD  G NALH++    +++L E+FL + D+ ++  + +  +A+HYAV    ++ I+E+L   +++ +  ++  N+   +PLM
Sbjct:   33 SASPAEAILAGYFAQVRYLLQTGGRGAADTREPLAGGRTPLILCSLVRDRCWSVSLARLLLEKGAKVGLKDRRGMNALHYACAHGREELAELFLQAIDYRVDAADGYRNSAMHYAVRG-GRLRIVEALAKVLQRLQLPVSPPNREGVTPLM 182

[Close]

BLAST of SMED30032529 vs. TrEMBL
Match: A0A267GAP7 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013982g1 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 8.183e-22
Identity = 52/151 (34.44%), Postives = 90/151 (59.60%), Query Frame = 2

Query:  155 ASSPAEAIYRGLFKQVRELLN----KEADPDEKIKS-KTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLM 592
            ++SPAEAI  G F QVR LL       AD  E +   +T ++LC+ + D  W+ SL+  LLE GAK+  KD  G NALH++    +++L E+FL + D+ ++  + +  +A+HYAV    ++ ++E+L   +++ +  ++  N+   +PLM
Sbjct:   14 SASPAEAILAGYFAQVRYLLQTGGRGAADTREPLAGGRTPLILCSLVRDRCWSVSLARLLLEKGAKVGLKDRRGMNALHYACAHGREELAELFLQAIDYRVDAADGYRNSAMHYAVRG-GRLRVVEALAKVLQRLQLPVSPPNREGVTPLM 163

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BLAST of SMED30032529 vs. TrEMBL
Match: A0A1S3KG74 (uncharacterized protein LOC106181577 OS=Lingula unguis OX=7574 GN=LOC106181577 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 3.434e-21
Identity = 55/159 (34.59%), Postives = 93/159 (58.49%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSK-TAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNG 646
            AI RG F+QVR L+   +D + K +   T ++LC  + +  W   LS  LLE GA +  +D+ G NALH++ +  + +LV +FL + D+D+N  + FG TALHYAV S   ++I   L+  ++KY  +++  N+   +PL+++         ++L+D G
Sbjct:   15 AIKRGFFQQVRFLIELGSDVNGKDEDGLTPLILCAMIEEEEWGVGLSRLLLEKGASVGFRDKRGLNALHYACLYQRLELVRVFLCAIDYDLNQGDKFGNTALHYAV-SVGNVDITRLLLRTLQKYGMTVDKPNRRGFTPLIQAWSTGRVTCAQILVDEG 172

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BLAST of SMED30032529 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310])

HSP 1 Score: 53.5286 bits (127), Expect = 3.534e-7
Identity = 40/140 (28.57%), Postives = 69/140 (49.29%), Query Frame = 2

Query:  185 GLFKQVRELLNKEADP-DEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESI 601
            G   +VREL+ K  D  D K + KTA+ +          K     LL+  + I+ KDE G  ALH++   N+ ++  + L S   ++N  NN   TALH AVN          ++  + ++   +N+ + + ++PL ++I
Sbjct:  499 GSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVK----VLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLL-SKGANVNLLNNSMCTALHIAVNKG-----FTDVVRVLTEHSADVNLQDSYGDTPLHDAI 628

[Close]

BLAST of SMED30032529 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310])

HSP 1 Score: 53.1434 bits (126), Expect = 3.729e-7
Identity = 40/140 (28.57%), Postives = 69/140 (49.29%), Query Frame = 2

Query:  185 GLFKQVRELLNKEADP-DEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESI 601
            G   +VREL+ K  D  D K + KTA+ +          K     LL+  + I+ KDE G  ALH++   N+ ++  + L S   ++N  NN   TALH AVN          ++  + ++   +N+ + + ++PL ++I
Sbjct:  505 GSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVK----VLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLL-SKGANVNLLNNSMCTALHIAVNKG-----FTDVVRVLTEHSADVNLQDSYGDTPLHDAI 634

[Close]

BLAST of SMED30032529 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310])

HSP 1 Score: 53.1434 bits (126), Expect = 3.963e-7
Identity = 44/160 (27.50%), Postives = 77/160 (48.12%), Query Frame = 2

Query:  125 FKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADP-DEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESI 601
              +S  ++N      EA + G   +VREL+ K  D  D K + KTA+ +          K     LL+  + I+ KDE G  ALH++   N+ ++  + L S   ++N  NN   TALH AVN          ++  + ++   +N+ + + ++PL ++I
Sbjct:  425 LSQSTEQDNPGRLVIEAAH-GSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVK----VLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLL-SKGANVNLLNNSMCTALHIAVNKG-----FTDVVRVLTEHSADVNLQDSYGDTPLHDAI 573

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BLAST of SMED30032529 vs. Ensembl Cavefish
Match: ankrd52a (ankyrin repeat domain 52a [Source:NCBI gene;Acc:553206])

HSP 1 Score: 52.7582 bits (125), Expect = 4.950e-7
Identity = 35/115 (30.43%), Postives = 55/115 (47.83%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDF 661
            LL  GA+ID+ + +G  ALH +    ++ +    +N    ++N  NN G+T LH A  S +    LE L+         +N+ +K   SPL  +  H  F   ++LI NG   D 
Sbjct:  216 LLRNGAEIDEPNAFGNTALHVACYMGQEAVANELVNR-GANVNQPNNRGYTPLHLAAVSTNGALCLELLV----NNGADVNMQSKEGKSPLHMAAIHGRFTRSQILIQNGGEIDC 325

[Close]

BLAST of SMED30032529 vs. Ensembl Cavefish
Match: ENSAMXT00000031513.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002164.1:626:64909:-1 gene:ENSAMXG00000032083.1 transcript:ENSAMXT00000031513.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 5.313e-7
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTD 658
            LL  GA+ID+ + +G  ALH +    ++ +    +N    ++N  NN G+T LH A  S +    LE L+         +N+ +K   SPL  +  H  F   ++LI NG   D
Sbjct:  219 LLRNGAEIDEPNAFGNTALHVACYTGQEAVANELVNR-GANVNQPNNRGYTPLHLAAVSTNGALCLELLV----NNGADVNMQSKEGKSPLHMAAIHGRFTRSQILIQNGGEID 327

[Close]

BLAST of SMED30032529 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 4.705e-8
Identity = 33/120 (27.50%), Postives = 55/120 (45.83%), Query Frame = 2

Query:  305 LSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            ++ TLLEYGA+ +     G   LH    +   D+  + L  +  D+N     G + LH A   E K+ + E L+    K+   +N   K   +PL+ +  + N   V  L+ +GA  + K
Sbjct:  261 IATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYA-DVNHATKSGLSPLHLAAQ-EDKVNVAEILV----KHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSK 374

[Close]

BLAST of SMED30032529 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 6.001e-8
Identity = 37/116 (31.90%), Postives = 53/116 (45.69%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            LL+ G  ID  + +G  ALH +   N +D+V   L     ++N  N  GFT LH+A  S      LE L+         +N+ +K   SPL  +  H  F   + LI NG   D +
Sbjct:  223 LLDLGVDIDDPNSFGNTALHVACY-NGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLV----NNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCE 333

[Close]

BLAST of SMED30032529 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 2.160e-6
Identity = 33/120 (27.50%), Postives = 55/120 (45.83%), Query Frame = 2

Query:  305 LSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            ++ TLLEYGA+ +     G   LH    +   D+  + L  +  D+N     G + LH A   E K+ + E L+    K+   +N   K   +PL+ +  + N   V  L+ +GA  + K
Sbjct:  351 IATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYA-DVNHATKSGLSPLHLAAQ-EDKVNVAEILV----KHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSK 464

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BLAST of SMED30032529 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 48.521 bits (114), Expect = 1.698e-6
Identity = 33/103 (32.04%), Postives = 55/103 (53.40%), Query Frame = 2

Query:  347 KDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNI--LNKFNNSPLMESIRHLNFHAVEVLIDNGA 649
            +D  G   L  +  K K D+V+  +    +DIN ++N G TALH A   +  IEI+E LI    +    +NI  +  F ++PL+++  + +   V+ L+ NGA
Sbjct:  326 RDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAA-LQGHIEIVELLI----ENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGA 423

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BLAST of SMED30032529 vs. Ensembl Nematostella
Match: EDO38263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDI0])

HSP 1 Score: 66.2402 bits (160), Expect = 9.164e-12
Identity = 45/162 (27.78%), Postives = 82/162 (50.62%), Query Frame = 2

Query:  173 AIYRGLFKQVR---ELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSF-DFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNG 646
            A+    F+Q +   E+ N + +  ++ K +TA+M   FL +   A  L+  LL +GA++  +D  G  AL ++    ++ LV   +     FD N  +  G TALHYA  S + + +L  ++  +K++K S +  N    +PL+ + +  NF    +L+  G
Sbjct:   50 AVKSKRFRQAKILIEMGNIDVNARDETK-RTALMELCFLEEENKAAKLARMLLSHGARVGIRDTDGMTALSYAAKLGREQLVSAMMEEVHSFDPNAADKHGNTALHYASMSGNFV-VLNLILKTLKRFKLSADKPNNAGETPLICASKTGNFLCARILVSEG 209

[Close]

BLAST of SMED30032529 vs. Ensembl Nematostella
Match: EDO41179 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S546])

HSP 1 Score: 62.3882 bits (150), Expect = 1.076e-10
Identity = 43/159 (27.04%), Postives = 88/159 (55.35%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKI-KSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNG 646
            AI +  ++Q R L+   AD + +  + +T +M   F+ +   A S++  LLE G  +  KD+ G  AL ++ I  KK L++      D+D+N  +  G TAL+YA+ +  +  ++E L+ ++K Y  +++ +N+   +PL+ +++       ++L++ G
Sbjct:    7 AIIQRRWRQARLLVEVGADVNRQGDEGRTPLMELCFVDNEDKACSMAKLLLENGGNVGIKDKQGKTALSYACILKKKKLLKWLTRFVDYDLNSTDLDGNTALNYAILAGDETVVME-LVRKLKAYGLTVDTINRKGETPLINALKSERTDIADILLEIG 164

[Close]

BLAST of SMED30032529 vs. Ensembl Nematostella
Match: EDO42585 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S176])

HSP 1 Score: 54.299 bits (129), Expect = 1.513e-9
Identity = 34/95 (35.79%), Postives = 58/95 (61.05%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSF-DFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMES 598
            L++ GAKI  KD++G NA+  +V+  K+ L+ +FL     F+IN ++  G TAL YA  S     IL  L+   +K++ S+++ NK   +PL+++
Sbjct:    8 LVDNGAKIALKDKHGRNAVMKAVLSEKEKLLTLFLTRVSSFNINDRDKKGNTALFYAA-SIGNFHILRKLVVVYRKFRLSVDVTNKDGMTPLIQA 101

[Close]

BLAST of SMED30032529 vs. Ensembl Nematostella
Match: EDO33642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRL7])

HSP 1 Score: 56.6102 bits (135), Expect = 1.047e-8
Identity = 40/166 (24.10%), Postives = 85/166 (51.20%), Query Frame = 2

Query:  158 SSPAEAIYRGLFKQVRELLNKEADPDEKIK--SKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYK-YSLNILNKFNNSPLMESIRHLNFHAVEVLIDNG 646
            SS A+A+    F+Q + L+    D + + K   ++ ++  + + D   +  +   LL+ GA I  +D++G NA      + +  +  +FL   DFD+N ++  G TAL YAV +   I    +++ +++K +    +  NK   +PL+ + +  +    ++L+++G
Sbjct:    2 SSLAKALEERRFRQAKLLVQLGDDLNSRSKETGRSPLVQASLIEDEDLSYKVCHWLLQEGADIAVRDDHGMNAFMHVCRQGRTRIAALFLKHRDFDLNTQDFEGNTALSYAVIA-GDISTTSAIVAELRKRRIRGADKQNKNGETPLIIAAKLGHKGCADILLNDG 166

[Close]

BLAST of SMED30032529 vs. Ensembl Nematostella
Match: EDO32718 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU82])

HSP 1 Score: 51.9878 bits (123), Expect = 4.536e-8
Identity = 45/169 (26.63%), Postives = 88/169 (52.07%), Query Frame = 2

Query:  170 EAIYRGLFKQVRELLNKEADPDEKI-KSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLN--SFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKY--SLNILNKFNN---SPLMESIRHLNFHAVEVLIDNGAR 652
             A+  G   QVR LL    + + +  + +TA++ C FL +    +++   LL  GAK++K+D  G +AL ++ ++   D+V   +N    + D+N  +N G T+L  AV S   +E++  L+  +++     + + +++ NN   SPL+ +    +     VL+  GA+
Sbjct:    5 SAVRFGRTHQVRLLLKAGINTENRDDRGRTALVYCCFLKNPQTRQTILKLLLASGAKVNKRDHLGRSALLWACLQGLVDVVTSLVNDSQSEVDLNLTDNDGNTSLLLAVKS-GNLEMVRLLLSLLRRSLSNNTTSFIHRCNNRGISPLLAAFLRGDKDCARVLVQGGAQ 172

[Close]

BLAST of SMED30032529 vs. Ensembl Medaka
Match: ENSORLT00000005069.2 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A [Source:NCBI gene;Acc:101168382])

HSP 1 Score: 53.5286 bits (127), Expect = 3.547e-7
Identity = 44/164 (26.83%), Postives = 71/164 (43.29%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            A Y G  + V+ L+   A+ D K K   + +       +    S    LL  G  +++ + YG   LH +   N +D+V   L     ++N  N  GF+ALH+A +S       E L+     +   +N  +K   +PL  +  H  F   + LI NGA  D +
Sbjct:  223 AAYMGHLEVVKLLVESGAEVDCKDKKGYSPL---HAAASSGMSSTVHYLLGLGVHVNEANSYGNTPLHLACY-NGQDVVVGELIQAGANVNQVNERGFSALHFASSSRQGALCQELLL----AHGACINSRSKDGKTPLHMAATHGRFSCSQALIQNGAEVDCE 378

[Close]

BLAST of SMED30032529 vs. Ensembl Medaka
Match: ENSORLT00000028696.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A [Source:NCBI gene;Acc:101168382])

HSP 1 Score: 53.1434 bits (126), Expect = 3.763e-7
Identity = 44/164 (26.83%), Postives = 71/164 (43.29%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            A Y G  + V+ L+   A+ D K K   + +       +    S    LL  G  +++ + YG   LH +   N +D+V   L     ++N  N  GF+ALH+A +S       E L+     +   +N  +K   +PL  +  H  F   + LI NGA  D +
Sbjct:  180 AAYMGHLEVVKLLVESGAEVDCKDKKGYSPL---HAAASSGMSSTVHYLLGLGVHVNEANSYGNTPLHLACY-NGQDVVVGELIQAGANVNQVNERGFSALHFASSSRQGALCQELLL----AHGACINSRSKDGKTPLHMAATHGRFSCSQALIQNGAEVDCE 335

[Close]

BLAST of SMED30032529 vs. Ensembl Medaka
Match: ENSORLT00000037670.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A [Source:NCBI gene;Acc:101168382])

HSP 1 Score: 53.1434 bits (126), Expect = 4.006e-7
Identity = 44/164 (26.83%), Postives = 71/164 (43.29%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            A Y G  + V+ L+   A+ D K K   + +       +    S    LL  G  +++ + YG   LH +   N +D+V   L     ++N  N  GF+ALH+A +S       E L+     +   +N  +K   +PL  +  H  F   + LI NGA  D +
Sbjct:  180 AAYMGHLEVVKLLVESGAEVDCKDKKGYSPL---HAAASSGMSSTVHYLLGLGVHVNEANSYGNTPLHLACY-NGQDVVVGELIQAGANVNQVNERGFSALHFASSSRQGALCQELLL----AHGACINSRSKDGKTPLHMAATHGRFSCSQALIQNGAEVDCE 335

[Close]

BLAST of SMED30032529 vs. Ensembl Medaka
Match: ENSORLT00000045754.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A [Source:NCBI gene;Acc:101168382])

HSP 1 Score: 53.1434 bits (126), Expect = 4.006e-7
Identity = 44/164 (26.83%), Postives = 71/164 (43.29%), Query Frame = 2

Query:  173 AIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK 664
            A Y G  + V+ L+   A+ D K K   + +       +    S    LL  G  +++ + YG   LH +   N +D+V   L     ++N  N  GF+ALH+A +S       E L+     +   +N  +K   +PL  +  H  F   + LI NGA  D +
Sbjct:  180 AAYMGHLEVVKLLVESGAEVDCKDKKGYSPL---HAAASSGMSSTVHYLLGLGVHVNEANSYGNTPLHLACY-NGQDVVVGELIQAGANVNQVNERGFSALHFASSSRQGALCQELLL----AHGACINSRSKDGKTPLHMAATHGRFSCSQALIQNGAEVDCE 335

[Close]

BLAST of SMED30032529 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479])

HSP 1 Score: 52.373 bits (124), Expect = 8.017e-7
Identity = 43/160 (26.88%), Postives = 76/160 (47.50%), Query Frame = 2

Query:  125 FKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADP-DEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESI 601
              +S  ++N      EA +    K VREL+ K  D  D K + KTA+ +          K+L    L+    ++ KDE G  ALH++   N+ ++  + L S   ++N  NN   TALH AVN          ++  + ++   +N+ + + ++PL ++I
Sbjct:  416 LSQSTEQDNPCRLVIEAAHGSAVK-VRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKAL----LQANCSVEVKDEDGDTALHYAAFGNQAEIARLLL-SKGANVNLLNNSMCTALHIAVNKG-----FTDVVRVLTEHSADVNLQDSYGDTPLHDAI 564

[Close]

BLAST of SMED30032529 vs. Planmine SMEST
Match: SMESG000077536.1 (SMESG000077536.1)

HSP 1 Score: 633.639 bits (1633), Expect = 0.000e+0
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 2

Query:  119 MNFKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFKYEGLSFLDYFNNLKRKLKETPEYMXXXXXXXXXXXXXEELAMHDSIIRSVEQISIEEAPDFDIRNNSKWVVERPKSCITLHSFNENEESSWKTIMPIVLNQLEERISKSYRKNFSVEKIISNLKQFNHEAGDTTXXXXXXXXXXXXXXXXXXXXXXXXXXQDKLSKRSTNDGIKINKF 1198
            MNFKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDIN KNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKF+NSPLMESIRHLNFHA EVLIDNGARTDFKYEGLSFLDYFNNLKRKLKETPEYMINKIKKSIIINKNEELAMHDSIIRSVEQISIEEAPDFDIRNNSKWVVERPKSCITLHSFNENEESSWKTIMPIVLNQLEERISKSYRKNFSVEKIISNLKQFNHEAGDTTGKGRNGSSKKSNAKSKLNKKGSASSSQDKLSKRSTNDGIKINKF
Sbjct:    1 MNFKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADPDEKIKSKTAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINSKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFSNSPLMESIRHLNFHAAEVLIDNGARTDFKYEGLSFLDYFNNLKRKLKETPEYMINKIKKSIIINKNEELAMHDSIIRSVEQISIEEAPDFDIRNNSKWVVERPKSCITLHSFNENEESSWKTIMPIVLNQLEERISKSYRKNFSVEKIISNLKQFNHEAGDTTGKGRNGSSKKSNAKSKLNKKGSASSSQDKLSKRSTNDGIKINKF 360

[Close]

BLAST of SMED30032529 vs. Planmine SMEST
Match: SMESG000034080.1 (SMESG000034080.1)

HSP 1 Score: 62.003 bits (149), Expect = 2.904e-10
Identity = 41/116 (35.34%), Postives = 68/116 (58.62%), Query Frame = 2

Query:  155 ASSPAEAIYRGLFKQVRELL-NKEADPDEKIKSK-TAIMLCTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSE 496
            +S   + I +G F Q ++ + N   D +E  ++K T ++LC+ + D  W  SL+  LLE GA I++ D+   N LH+  I  +K+L+++ L S DF+IN  +  G + L YA+ SE
Sbjct:    7 SSDICKLILKGYFAQAKDKIKNTNVDVNETNENKQTPLILCSLIEDPSWGISLAQLLLELGADIERADDNKMNVLHYCAIYQQKELLKLLLESVDFNINKGDILGNSPLIYAIVSE 122

[Close]

BLAST of SMED30032529 vs. Planmine SMEST
Match: SMESG000058588.1 (SMESG000058588.1)

HSP 1 Score: 56.225 bits (134), Expect = 3.834e-8
Identity = 44/132 (33.33%), Postives = 68/132 (51.52%), Query Frame = 2

Query:  317 LLEYGAKIDKK-DEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGAR-TDFKYEGLSFLDYFNNLK 706
            L  YGA  + K ++     LH + I     ++ +FL++ + +IN  +    T L YA   +S I+ +ESLI    +    +N  N   N+PL E++RH N   +E+LI +GA  T     GLS LD   +LK
Sbjct:  882 LFHYGADPNTKTNKLQLTPLHLAAIYGSIKVLNLFLHNANVNINAADKKLNTPLAYACAKDS-IDTVESLI----QAGALINTNNYKGNTPLHEAVRHNNASIIELLIKHGANITTLNANGLSPLDLSKDLK 1008

[Close]

BLAST of SMED30032529 vs. Planmine SMEST
Match: SMESG000058588.1 (SMESG000058588.1)

HSP 1 Score: 56.225 bits (134), Expect = 3.848e-8
Identity = 44/132 (33.33%), Postives = 68/132 (51.52%), Query Frame = 2

Query:  317 LLEYGAKIDKK-DEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGAR-TDFKYEGLSFLDYFNNLK 706
            L  YGA  + K ++     LH + I     ++ +FL++ + +IN  +    T L YA   +S I+ +ESLI    +    +N  N   N+PL E++RH N   +E+LI +GA  T     GLS LD   +LK
Sbjct:  902 LFHYGADPNTKTNKLQLTPLHLAAIYGSIKVLNLFLHNANVNINAADKKLNTPLAYACAKDS-IDTVESLI----QAGALINTNNYKGNTPLHEAVRHNNASIIELLIKHGANITTLNANGLSPLDLSKDLK 1028

[Close]

BLAST of SMED30032529 vs. Planmine SMEST
Match: SMESG000026655.1 (SMESG000026655.1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.592e-6
Identity = 41/149 (27.52%), Postives = 74/149 (49.66%), Query Frame = 2

Query:  317 LLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIFLNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILNKFNNSPLMESIRHLNFHAVEVLIDNGARTDFK-YEGLSFLD------YFNNLK--RKLKETPEYM 736
            LL+  A ++ + E G  ALH +  KN+ D+VE  L + + DI+ K   G+TALH AV+   +I+ ++ L+    +  + +N       + L  + +  +   V +L+DN    + +  +GL+ L+      Y N  +   K+   PE M
Sbjct:  717 LLDANADVNHQAENGLTALHLAAEKNRTDIVEQLLKT-EADISTKTQMGYTALHTAVHF-GQIQTVKMLL----EKNFDVNAQTNLGCTSLHLAAQQGHIAIVRLLLDNEIDVNHQNKQGLTALEVAQKQHYLNIAETLSKVTTVPEMM 859

The following BLAST results are available for this feature:

BLAST of SMED30032529 vs. Ensembl Human
BLAST of SMED30032529 vs. Ensembl Celegans
BLAST of SMED30032529 vs. Ensembl Fly
BLAST of SMED30032529 vs. Ensembl Zebrafish
BLAST of SMED30032529 vs. Ensembl Xenopus
BLAST of SMED30032529 vs. Ensembl Mouse
BLAST of SMED30032529 vs. UniProt/SwissProt
BLAST of SMED30032529 vs. TrEMBL
BLAST of SMED30032529 vs. Ensembl Cavefish
BLAST of SMED30032529 vs. Ensembl Sea Lamprey
BLAST of SMED30032529 vs. Ensembl Yeast
BLAST of SMED30032529 vs. Ensembl Nematostella
BLAST of SMED30032529 vs. Ensembl Medaka
BLAST of SMED30032529 vs. Planmine SMEST

BLAST of SMED30032529 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ANKRD44	6.545e-8	35.09	ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD44	6.851e-8	34.78	ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD44	6.933e-8	35.09	ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD44	7.212e-8	35.09	ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD44	7.623e-8	35.09	ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H...	[more]

BLAST of SMED30032529 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032529 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Kank	6.256e-7	30.40	gene:FBgn0027596 transcript:FBtr0303758	[more]
Kank	6.763e-7	31.62	gene:FBgn0027596 transcript:FBtr0303756	[more]
Kank	7.805e-7	31.62	gene:FBgn0027596 transcript:FBtr0087431	[more]
Kank	8.146e-7	31.62	gene:FBgn0027596 transcript:FBtr0332856	[more]
Kank	9.751e-7	31.62	gene:FBgn0027596 transcript:FBtr0087430	[more]

BLAST of SMED30032529 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CABZ01071939.1	9.475e-7	31.36	pep chromosome:GRCz11:19:2018796:2029777:-1 gene:E...	[more]
ankrd44	1.017e-6	30.43	ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE...	[more]
ankrd44	1.060e-6	30.43	ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE...	[more]
rai14	1.493e-6	26.42	retinoic acid induced 14 [Source:ZFIN;Acc:ZDB-GENE...	[more]
rai14	1.527e-6	26.42	retinoic acid induced 14 [Source:ZFIN;Acc:ZDB-GENE...	[more]

BLAST of SMED30032529 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
psmd10	3.345e-8	27.07	proteasome 26S subunit, non-ATPase 10 [Source:NCBI...	[more]
ankrd52	2.629e-6	31.58	ankyrin repeat domain 52 [Source:NCBI gene;Acc:100...	[more]
ankrd52	2.647e-6	31.58	ankyrin repeat domain 52 [Source:NCBI gene;Acc:100...	[more]
ankrd52	3.017e-6	31.58	ankyrin repeat domain 52 [Source:NCBI gene;Acc:100...	[more]
ankrd52	3.078e-6	31.58	ankyrin repeat domain 52 [Source:NCBI gene;Acc:100...	[more]

BLAST of SMED30032529 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ankrd44	3.178e-8	34.78	ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd44	3.364e-8	34.78	ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd44	4.315e-8	35.09	ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG...	[more]
Ankrd52	1.636e-7	33.33	ankyrin repeat domain 52 [Source:MGI Symbol;Acc:MG...	[more]
A26c3	3.385e-6	27.88	ANKRD26-like family C, member 3 [Source:MGI Symbol...	[more]

BLAST of SMED30032529 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|B2RXR6\|ANR44_MOUSE	2.223e-7	34.78	Serine/threonine-protein phosphatase 6 regulatory ...	[more]
sp\|Q8N8A2\|ANR44_HUMAN	3.290e-7	34.78	Serine/threonine-protein phosphatase 6 regulatory ...	[more]
sp\|Q5F478\|ANR44_CHICK	4.499e-7	32.46	Serine/threonine-protein phosphatase 6 regulatory ...	[more]
sp\|Q4UMH6\|Y381_RICFE	6.080e-7	26.54	Putative ankyrin repeat protein RF_0381 OS=Rickett...	[more]
sp\|Q8BTI7\|ANR52_MOUSE	1.145e-6	33.33	Serine/threonine-protein phosphatase 6 regulatory ...	[more]

BLAST of SMED30032529 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267DSQ1	4.750e-22	35.10	ANK_REP_REGION domain-containing protein (Fragment...	[more]
A0A267E3L7	5.222e-22	35.10	ANK_REP_REGION domain-containing protein (Fragment...	[more]
A0A1I8H035	5.726e-22	35.10	ANK_REP_REGION domain-containing protein OS=Macros...	[more]
A0A267GAP7	8.183e-22	34.44	ANK_REP_REGION domain-containing protein OS=Macros...	[more]
A0A1S3KG74	3.434e-21	34.59	uncharacterized protein LOC106181577 OS=Lingula un...	[more]

BLAST of SMED30032529 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mib2	3.534e-7	28.57	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]
mib2	3.729e-7	28.57	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]
mib2	3.963e-7	27.50	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]
ankrd52a	4.950e-7	30.43	ankyrin repeat domain 52a [Source:NCBI gene;Acc:55...	[more]
ENSAMXT00000031513.1	5.313e-7	30.70	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of SMED30032529 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSPMAT00000007449.1	4.705e-8	27.50	pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1...	[more]
ENSPMAT00000003991.1	6.001e-8	31.90	pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1...	[more]
ENSPMAT00000007446.1	2.160e-6	27.50	pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1...	[more]

BLAST of SMED30032529 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
HOS4	1.698e-6	32.04	Subunit of the Set3 complex; complex is a meiotic-...	[more]

BLAST of SMED30032529 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO38263	9.164e-12	27.78	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41179	1.076e-10	27.04	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42585	1.513e-9	35.79	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33642	1.047e-8	24.10	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32718	4.536e-8	26.63	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30032529 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000005069.2	3.547e-7	26.83	serine/threonine-protein phosphatase 6 regulatory ...	[more]
ENSORLT00000028696.1	3.763e-7	26.83	serine/threonine-protein phosphatase 6 regulatory ...	[more]
ENSORLT00000037670.1	4.006e-7	26.83	serine/threonine-protein phosphatase 6 regulatory ...	[more]
ENSORLT00000045754.1	4.006e-7	26.83	serine/threonine-protein phosphatase 6 regulatory ...	[more]
mib2	8.017e-7	26.88	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]

BLAST of SMED30032529 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000077536.1	0.000e+0	99.17	SMESG000077536.1	[more]
SMESG000034080.1	2.904e-10	35.34	SMESG000034080.1	[more]
SMESG000058588.1	3.834e-8	33.33	SMESG000058588.1	[more]
SMESG000058588.1	3.848e-8	33.33	SMESG000058588.1	[more]
SMESG000026655.1	3.592e-6	27.52	SMESG000026655.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30032529 ID=SMED30032529|Name=SMED30032529|organism=Schmidtea mediterranea sexual|type=transcript|length=1253bp
TTTATTGAAATTATTGATTAAATAGCAGAATATGTAGAAATTCAGGTAGT
AATACAAATATCAAATATCTGATAAAAATTAATTCGTAAGCCAATTTTAA
CGAAAATGATTTAGTGAAATGAATTTTAAAAGATCCATCTTTCGAAATAA
TCAGGCTTCTAGCCCAGCTGAAGCCATTTACAGAGGACTATTTAAACAGG
TCAGAGAGTTGTTGAACAAAGAAGCTGACCCAGATGAAAAAATCAAAAGC
AAGACCGCTATTATGCTATGTACTTTTCTCACTGATACTTTTTGGGCAAA
AAGTCTTTCATTTACACTATTAGAATATGGAGCTAAAATTGACAAAAAAG
ATGAATACGGATTCAACGCGTTGCACTTTTCCGTCATTAAAAATAAAAAA
GACTTAGTGGAAATATTTCTTAATTCATTTGATTTTGATATTAATGGTAA
AAACAATTTTGGTTTTACTGCCTTACATTATGCCGTGAATTCTGAAAGTA
AAATAGAGATTCTAGAAAGTCTCATTTTCCAAATGAAAAAATATAAATAT
TCTTTAAATATTTTAAATAAATTCAATAATTCACCACTAATGGAATCGAT
TCGGCATTTGAACTTTCACGCAGTGGAAGTTTTAATTGATAATGGTGCCA
GAACTGATTTCAAATATGAAGGTCTATCGTTTTTAGATTATTTCAATAAT
TTGAAAAGAAAATTAAAAGAAACCCCAGAATATATGATAAATAAAATAAA
AAAATCAATCATCATTAATAAAAATGAAGAATTAGCAATGCATGATTCTA
TAATCCGGTCAGTTGAACAAATATCCATCGAGGAAGCTCCAGACTTCGAT
ATCAGGAACAATTCTAAATGGGTTGTGGAACGACCTAAATCATGCATAAC
GTTACATTCTTTTAATGAAAACGAGGAAAGTTCTTGGAAAACTATAATGC
CAATTGTTCTTAATCAGCTAGAAGAAAGAATTTCCAAATCTTACAGAAAG
AATTTTAGCGTAGAAAAAATAATTTCGAATTTAAAACAATTTAATCACGA
AGCAGGAGATACGACAGGTAAAGGAAGAAATGGATCCTCAAAAAAGTCCA
ATGCAAAATCTAAATTAAACAAAAAAGGTTCGGCTTCCTCCAGTCAAGAT
AAATTATCCAAAAGGTCAACAAATGATGGAATAAAGATAAATAAATTTTA
ATACCATTCTGCTAAAATTGAATTTTTATCATTGTATAATAATAAATTAA
TAG

protein sequence of SMED30032529-orf-1

>SMED30032529-orf-1 ID=SMED30032529-orf-1|Name=SMED30032529-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=361bp
MNFKRSIFRNNQASSPAEAIYRGLFKQVRELLNKEADPDEKIKSKTAIML
CTFLTDTFWAKSLSFTLLEYGAKIDKKDEYGFNALHFSVIKNKKDLVEIF
LNSFDFDINGKNNFGFTALHYAVNSESKIEILESLIFQMKKYKYSLNILN
KFNNSPLMESIRHLNFHAVEVLIDNGARTDFKYEGLSFLDYFNNLKRKLK
ETPEYMINKIKKSIIINKNEELAMHDSIIRSVEQISIEEAPDFDIRNNSK
WVVERPKSCITLHSFNENEESSWKTIMPIVLNQLEERISKSYRKNFSVEK
IISNLKQFNHEAGDTTGKGRNGSSKKSNAKSKLNKKGSASSSQDKLSKRS
TNDGIKINKF*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000420	parapharyngeal region
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036770	Ankyrin_rpt-contain_sf
IPR002110	Ankyrin_rpt
IPR020683	Ankyrin_rpt-contain_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0016301	kinase activity

Vocabulary: **biological process**
Term	Definition
GO:0016310	phosphorylation

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 114..148 e-value: 92.0 score: 11.9 coord: 80..110 e-value: 0.33 score: 20.0 coord: 152..183 e-value: 49.0 score: 12.8
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	G3DSA:1.25.40.20		coord: 9..236 e-value: 4.6E-23 score: 83.4
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	SSF48403	Ankyrin repeat	coord: 19..182
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 67..137 e-value: 1.4E-7 score: 32.0
IPR020683	Ankyrin repeat-containing domain	PROSITE	PS50297	ANK_REP_REGION	coord: 67..184 score: 18.855
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 312..360
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 312..348
None	No IPR available	PANTHER	PTHR24134	FAMILY NOT NAMED	coord: 17..124 coord: 66..157

Main menu

SMED30032529

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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