SMED30032528
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032528 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Alignments
SMED30032528 aligns in the following genomic locations:
Homology
BLAST of SMED30032528 vs. TrEMBL
Match: A0A1S3J6U5 (sodium-dependent glucose transporter 1-like OS=Lingula unguis OX=7574 GN=LOC106170691 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 1.824e-11 Identity = 51/137 (37.23%), Postives = 75/137 (54.74%), Query Frame = -3 Query: 250 EIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGV 660 E+ PTL +L +K++ SYE + R + RS+G + V G++ D FP +V ISVA F+ + T+ IPWC + + +L Q + +N G L + EK M H G+GIGGL S I++PFLS V Sbjct: 11 ELPGPTLKDLMKKVDSSYEEISRALIARSVGFFISSFVGGFLCDRFPKHVDIFISVAMFVNAVTTVSIPWCHELIPLAILMHFQGLSEGVLNTGGMTTCLNLWGEKVSAPMHSTHFGWGIGGLFSTQIARPFLSPDV 147
BLAST of SMED30032528 vs. TrEMBL
Match: A0A4Z2CR48 (Sodium-dependent glucose transporter 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_008203 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 2.873e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = -3 Query: 193 VLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGVSRNAKITKY--CRKVLNETEL 699 VL + I+ GL+ E+ PTLP L N Y+ +G +S R++G + ++ G++ D + A I ++ +G + TLIIPWC ++ ++ FI + ++ ++EKA G + H GYG+G ++ L+++PF +S N+ I + N TEL Sbjct: 26 VLCYAWISLGLYSEVLGPTLPTLMHNTNSDYDQIGMALSTRAIGLLFGSLIGGFLSDRYKALRAIFIMISLGVGAITTLIIPWCTNVIILTIVLFIAGLSHGFLTTNGNPLLASIWEEKAAGPFSLMHSGYGMGAAIAPLLAKPF---TISFNSSINNHSIVSNGTNSTEL 193
BLAST of SMED30032528 vs. TrEMBL
Match: Q5DF05 (SJCHGC08251 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 3.274e-10 Identity = 34/109 (31.19%), Postives = 58/109 (53.21%), Query Frame = -3 Query: 382 VLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFI---QSGFQNSMNN 699 VL + I+ GL+ E+ PTLP L N Y+ +G +S R++G + ++ G++ D + A I ++ +G + TLIIPWC ++ ++ FI GF + N Sbjct: 33 VLCYAWISLGLYSEVLGPTLPTLMHNTNSDYDQIGMALSTRAIGLLFGSLIGGFLSDRYKALRAIFIMISLGVGAITTLIIPWCTNVIILTIVLFIAGLSHGFLTTNGN 141
BLAST of SMED30032528 vs. TrEMBL
Match: K1QDJ2 (Sodium-dependent glucose transporter 1 OS=Crassostrea gigas OX=29159 GN=CGI_10009068 PE=4 SV=1) HSP 1 Score: 71.2478 bits (173), Expect = 3.483e-10 Identity = 47/148 (31.76%), Postives = 81/148 (54.73%), Query Frame = -3 Query: 259 LVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLS 702 L L+ + I GL+ EI PT +LK + ++ YE + R MS RS+G + G++ D Y M++V +G T++ P ++ L+ LF +Q F+ +N Q ++L+ ++EKA + + H G+GIG + I+ PFL+ Sbjct: 26 LFLVSVWICLGLYLEITGPTQKDLKLRAHLDYEEVSRAMSGRSIGFFIGAAIGGFLVDKLDPYCDLMVAVCLDLGATATIVAPHAQEIALLWFLFVMQGTFEGIINIAGQKLVLEIWQEKASSPLFLLHFGFGIGSFIVPQIANPFLA 173
BLAST of SMED30032528 vs. TrEMBL
Match: R7UAH2 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_222889 PE=4 SV=1) HSP 1 Score: 67.0106 bits (162), Expect = 3.689e-9 Identity = 40/155 (25.81%), Postives = 85/155 (54.84%), Query Frame = -3 Query: 238 IFIGIAP---GLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGVSRNA 693 +F+ +AP G++ E+ PT+ L +++ +YE + + + RS+G + +++G++ D F ++ ++S+A +G T++ PWC L+ V+F IQ Q ++ + L + A + H G+GIG ++ L+++PF+ + N+ Sbjct: 21 VFMVLAPTVMGIYLELIGPTMQELMNRVDANYEEISQALVARSVGYLLGSLISGFLADRFKGHLELLLSIAMLLGATGTMLSPWCTVLGLLAVMFLIQGLGQGMIDTSNNTLCLLMWGAVASAPIHALHFGFGIGAFIAPLLARPFIGDNNTSNS 175
BLAST of SMED30032528 vs. Planmine SMEST
Match: SMESG000076465.1 (SMESG000076465.1) HSP 1 Score: 413.69 bits (1062), Expect = 4.049e-144 Identity = 249/251 (99.20%), Postives = 250/251 (99.60%), Query Frame = -3 Query: 1 MSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGVSRNAKITKYCRKVLNETELGQXXXXXXXXXXXXXXIENKRLSFEYIYXXXXXXXXXXXXVNFAYSFLPNPDAEKYQIKKREPW 753 MSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHLGYGIGGLLSGLISQPFLSK VSRNAKITKYCRKVLNETELGQIVPSIIGSNISSIQIEN+RLSFEYIYVIAGVVAILIAIVNFAYSFLPNPDAEKYQIKKREPW Sbjct: 1 MSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHLGYGIGGLLSGLISQPFLSKSVSRNAKITKYCRKVLNETELGQIVPSIIGSNISSIQIENRRLSFEYIYVIAGVVAILIAIVNFAYSFLPNPDAEKYQIKKREPW 251
BLAST of SMED30032528 vs. Planmine SMEST
Match: SMESG000076464.1 (SMESG000076464.1) HSP 1 Score: 308.145 bits (788), Expect = 9.757e-103 Identity = 176/251 (70.12%), Postives = 207/251 (82.47%), Query Frame = -3 Query: 1 MSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGVSRNAKITKYCRKVLNETELGQXXXXXXXXXXXXXXIENKRLSFEYIYXXXXXXXXXXXXVNFAYSFLPNPDAEKYQIKKREPW 753 MSI++ K +YQNGLLL+L+ +GI+PGLW EI TPTLPNLKEKLNVSYE+MG+MMS+RS GNI ALI TGYVYD+FP+YV + S ACF GG CTL+IPWCKQFW+VVVLFFIQS QN M NGAQ+ I++ FK+KAV KMQIYHLGYGIGGLLSGLISQPFL+K +S+ AKIT YCRK+LNET Q S +NISS QIENK LSFEYIY+I GV+A+L AI N +YSF+P P+ +K QIKKREPW Sbjct: 1 MSIKVPSYKTFYQNGLLLILVCLGISPGLWDEIVTPTLPNLKEKLNVSYEIMGKMMSMRSFGNIVALITTGYVYDTFPSYVAYITSAACFTGGCCTLLIPWCKQFWVVVVLFFIQSAAQNFMTNGAQVTIMRIFKDKAVAKMQIYHLGYGIGGLLSGLISQPFLTKEISKKAKITNYCRKLLNETGFNQFGSSSPRNNISSSQIENKNLSFEYIYLITGVIAMLFAITNISYSFVPKPNKDKCQIKKREPW 251
BLAST of SMED30032528 vs. Planmine SMEST
Match: SMESG000076463.1 (SMESG000076463.1) HSP 1 Score: 145.976 bits (367), Expect = 2.613e-40 Identity = 74/174 (42.53%), Postives = 116/174 (66.67%), Query Frame = -3 Query: 187 LLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYHXXXXXXXXXXXXXSQPFLSKGVSRNAKITKYCRKVLNETELGQ 708 L+ +L+ +G+ G+ QE+ TPTLPN++ + NVSYE +G ++SLR +GNI + Y+YDS+P + + C I G+CT++IPWC+ F LV + + QSGF ++++N QL +L+ FK K++ +MQ+YH G+GIG LLSG I+ PF++ +K+TKYC + L L Sbjct: 16 LIAILVMLGLVIGICQEMMTPTLPNIRSQFNVSYETLGTVLSLRGLGNIIFTLSASYLYDSYPQSLNHFMISECIISGVCTILIPWCRYFSLVTFVIYFQSGFLSALHNACQLTVLREFKTKSISRMQLYHTGFGIGRLLSGFIAAPFVANKSGGLSKLTKYCNEKLQLERLND 189
BLAST of SMED30032528 vs. Planmine SMEST
Match: SMESG000015183.1 (SMESG000015183.1) HSP 1 Score: 53.9138 bits (128), Expect = 5.251e-8 Identity = 41/159 (25.79%), Postives = 70/159 (44.03%), Query Frame = -3 Query: 316 EQVD*NYNEILGQMSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEVMGRMMSLRSMGNIAALIVTGYVYDSFPAYVGSMISVACFIGGLCTLIIPWCKQFWLVVVLFFIQSGFQNSMNNGAQLIILKTFKEKAVGKMQIYH 792 +++ +NEI S RIT LK Y+ VL I+ GL+ EI P LP L+ ++E + R +S R+ G + ++ G + D F + +I + + G+ IP C+ ++ FI + + +KEK+ G H Sbjct: 21 NKIENGFNEI--SQSKRITHLK--YRLIKTFVLFLSWISLGLFSEIIGPALPALRIISGTNHEEISRALSSRAAGLLIGSLIGGVLADQFIKFRNLLIFIFLSLAGITVATIPLCQSLLILAFNLFISGFSHGAFTTSGNPSLGALWKEKSTGPFNFMH 175 The following BLAST results are available for this feature:
BLAST of SMED30032528 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032528 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032528 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30032528 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032528 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032528 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032528 ID=SMED30032528|Name=SMED30032528|organism=Schmidtea mediterranea sexual|type=transcript|length=854bpback to top protein sequence of SMED30032528-orf-1 >SMED30032528-orf-1 ID=SMED30032528-orf-1|Name=SMED30032528-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp MSIRITFLKNWYQNGLLLVLIFIGIAPGLWQEIFTPTLPNLKEKLNVSYEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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