Coiled-coil domain-containing protein 174

Overview
NameCoiled-coil domain-containing protein 174
Smed IDSMED30032505
Length (bp)941
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Coiled-coil domain-containing protein 174 (SMED30032505) t-SNE clustered cells

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein 174 (SMED30032505) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Coiled-coil domain-containing protein 174 (SMED30032505) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein 174 (SMED30032505) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032505

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30032505SMESG000018130.1 dd_Smed_v4_7657_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30032505SMESG000018130.1 dd_Smed_v4_7657_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Human
Match: CCDC174 (coiled-coil domain containing 174 [Source:HGNC Symbol;Acc:HGNC:28033])

HSP 1 Score: 62.3882 bits (150), Expect = 2.953e-10
Identity = 49/169 (28.99%), Postives = 76/169 (44.97%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTK------------YNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID---DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D   R +QM+ L   +++   +++    ++E     LE+RL K++ K             N D D    LP        E  ++ T  P       +++ + R++TK      REWDRGK+  F    K+   LR +RD  FAPP+ Y
Sbjct:  235 YEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRTKRENIKEKRKAILEARLAKLRQKKMKKSKEGGTEEENRDGDVIGPLP-------PEPEAVPTPRPAAQSSKVEVIVQERKDTKPGVPHIREWDRGKEFSFGYWSKRQSDLRAERDPEFAPPSDY 396
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Celegans
Match: C37A2.8 (pep chromosome:WBcel235:I:6798232:6799674:-1 gene:WBGene00016494.1 transcript:C37A2.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C37A2.8)

HSP 1 Score: 51.6026 bits (122), Expect = 3.047e-7
Identity = 42/143 (29.37%), Postives = 74/143 (51.75%), Query Frame = 3 
Query:  450 EIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSID-TSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            E R +GT + + S + +KRKE++E+L +   +   +K+   + ++  ++    +L K++ K+ +++  + + P   PA     + ID  S+P+       E R     KS REWDRGK      I+K+ R +RD  F PP  Y
Sbjct:  198 EQRVYGTSHMRLSLNENKRKEEIEKLLEMSKKTDMDKAKKKETEKHRDILRREKLNKLRAKHGLELLPEIVEPVAVPA-----SQIDLNSIPLPGQKETPEERHARLMKSDREWDRGKGHYTTWIEKQ-RDERDDEFRPPDSY 334
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Zebrafish
Match: ccdc174 (coiled-coil domain containing 174 [Source:ZFIN;Acc:ZDB-GENE-050320-54])

HSP 1 Score: 71.2478 bits (173), Expect = 2.017e-13
Identity = 45/162 (27.78%), Postives = 81/162 (50.00%), Query Frame = 3 
Query:  426 FYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTS--------IDTSVPVVIDDLLFEI--RRNTKSKSK--REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
             Y  + + E R+ G GYF F+ D  +R++Q E L   +D+  +++S   +++E     LE+RL K++ +   +   +    ++ PA D  +          +    P  +  +  EI  R++TK      REWDRGK+  +     + R++RD+ FAPP+ Y
Sbjct:  224 HYENIRDQEARELGVGYFAFAHDEDQRRKQRETLDMLRDQTTEQRSKREQLKEKRKALLEARLAKVRMRRMKNGKMEPGDEQVAPAADVAEIDEDDKDEDLVGPPAPAKVPKVEVEIQERKDTKPGVPHVREWDRGKEFSWGQWTSRRREERDSEFAPPSAY 385
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Xenopus
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:100379829])

HSP 1 Score: 62.7734 bits (151), Expect = 1.720e-10
Identity = 45/158 (28.48%), Postives = 75/158 (47.47%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEI----RRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D + R++QME L   +D+   ++    +++      L++RL K+K +   ++  +    +  P + +    +      V  +   E+    RR+TK      REWDRGK+  F +  K     R +RD  FAPP+FY
Sbjct:  227 YEDIRENEARQLGVGYFGFARDQTDRQKQMETLTMLRDQTEDQRLKRERLKAKRKSMLQARLSKLKQRKLKNLKGEGAEGETVPGEWAHPLFVGQPEEPVGPEKKLEVVVQERRDTKPGVPHVREWDRGKEFTFGHWSKPRPDPRDERDPEFAPPSFY 384
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Xenopus
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:100379829])

HSP 1 Score: 59.6918 bits (143), Expect = 2.040e-9
Identity = 47/163 (28.83%), Postives = 77/163 (47.24%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKD---------SEDTSIDTSVPVVIDDLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D + R++QME L   +D+   ++    +++      L++RL K+K +   ++  +    +  P +D          E    +  + VV+ +     RR+TK      REWDRGK+  F +  K     R +RD  FAPP+FY
Sbjct:  179 YEDIRENEARQLGVGYFGFARDQTDRQKQMETLTMLRDQTEDQRLKRERLKAKRKSMLQARLSKLKQRKLKNLKGEGAEGETVPEEDFIGPELPEPEEPVGPEKKLEVVVQE-----RRDTKPGVPHVREWDRGKEFTFGHWSKPRPDPRDERDPEFAPPSFY 336
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Xenopus
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:100379829])

HSP 1 Score: 58.9214 bits (141), Expect = 3.860e-9
Identity = 44/160 (27.50%), Postives = 77/160 (48.12%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID------DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D + R++QME L   +D+   ++    +++      L++RL K+K +   ++  +    +  P ++ +    +   P          +++ + RR+TK      REWDRGK+  F +  K     R +RD  FAPP+FY
Sbjct:  229 YEDIRENEARQLGVGYFGFARDQTDRQKQMETLTMLRDQTEDQRLKRERLKAKRKSMLQARLSKLKQRKLKNLKGEGAEGETVPGEEEDFIGPELPEPEEPVGPEKKLEVVVQERRDTKPGVPHVREWDRGKEFTFGHWSKPRPDPRDERDPEFAPPSFY 388
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Mouse
Match: Ccdc174 (coiled-coil domain containing 174 [Source:MGI Symbol;Acc:MGI:2444652])

HSP 1 Score: 56.225 bits (134), Expect = 1.893e-8
Identity = 48/170 (28.24%), Postives = 73/170 (42.94%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID----------------DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D   R +QM+ L   +++   ++     ++E     LE+RL K++ K  +    +D         + E    D  VP   +                +++ + RR+TK      REWDRGK   F    KK   LR +RD  FAPP+ Y
Sbjct:  236 YEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRIKRENIKEKRKAMLEARLAKLRQK-KMKKSKED-------GTEEEGREADGVVPEPSEPKPVPAPAPVAQNSKVEVIIQERRDTKPGVPHIREWDRGKDFSFGFWSKKQSELRAERDPEFAPPSNY 397
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. UniProt/SwissProt
Match: sp|Q6PII3|CC174_HUMAN (Coiled-coil domain-containing protein 174 OS=Homo sapiens OX=9606 GN=CCDC174 PE=1 SV=3)

HSP 1 Score: 62.3882 bits (150), Expect = 1.418e-9
Identity = 49/169 (28.99%), Postives = 76/169 (44.97%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTK------------YNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID---DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D   R +QM+ L   +++   +++    ++E     LE+RL K++ K             N D D    LP        E  ++ T  P       +++ + R++TK      REWDRGK+  F    K+   LR +RD  FAPP+ Y
Sbjct:  235 YEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRTKRENIKEKRKAILEARLAKLRQKKMKKSKEGGTEEENRDGDVIGPLP-------PEPEAVPTPRPAAQSSKVEVIVQERKDTKPGVPHIREWDRGKEFSFGYWSKRQSDLRAERDPEFAPPSDY 396
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. UniProt/SwissProt
Match: sp|Q5PQS7|CC174_RAT (Coiled-coil domain-containing protein 174 OS=Rattus norvegicus OX=10116 GN=Ccdc174 PE=2 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 2.657e-9
Identity = 48/161 (29.81%), Postives = 75/161 (46.58%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKT----KYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID---DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D   R +QM+ L   +++   ++     ++E     LE+RL K++     K  VD   ++        + SE  S+    PV      +++ + R+++K      REWDRGK   F    KK   LR +RD  FAPP+ Y
Sbjct:  244 YEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRIKRENIKEKRKAILEARLAKLRQKKMKKSKVDGTEEESRADGVVTEPSEPKSVPAPPPVAQSSKVEVIIQERKDSKPGVPHIREWDRGKDFSFGFWSKKQSELRAERDPEFAPPSNY 404
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. UniProt/SwissProt
Match: sp|Q5ZIH9|CC174_CHICK (Coiled-coil domain-containing protein 174 OS=Gallus gallus OX=9031 GN=CCDC174 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.835e-9
Identity = 48/165 (29.09%), Postives = 81/165 (49.09%), Query Frame = 3 
Query:  426 FYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID----------DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
             Y  +   E R  G GYF FS D   R +Q   L   +++ + +++   +++E     LESRL K++ +    +  + L  + +  ++ E    DT+ PV  +          +++ + RR+TK      REWD+GK+ +F    KK   LR +RD  FAPP+ Y
Sbjct:  233 HYEDIRENEARQLGVGYFAFSRDQDLRHKQRATLDMLREQTLDQRTKREQLKEKRKAALESRLSKLRARKVKKLREEGLEEEAERLENGE--VKDTTSPVEAEPEVPRPSRKVEVVIQERRDTKPGVPYVREWDKGKELMFGQWSKKQEELRDERDPEFAPPSDY 395
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. UniProt/SwissProt
Match: sp|Q3U155|CC174_MOUSE (Coiled-coil domain-containing protein 174 OS=Mus musculus OX=10090 GN=Ccdc174 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.232e-7
Identity = 48/170 (28.24%), Postives = 73/170 (42.94%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVID----------------DLLFEIRRNTKSKSK--REWDRGKKTIFENIQKK---LRKDRDTNFAPPAFY 875
            Y  +   E R  G GYF F+ D   R +QM+ L   +++   ++     ++E     LE+RL K++ K  +    +D         + E    D  VP   +                +++ + RR+TK      REWDRGK   F    KK   LR +RD  FAPP+ Y
Sbjct:  236 YEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRIKRENIKEKRKAMLEARLAKLRQK-KMKKSKED-------GTEEEGREADGVVPEPSEPKPVPAPAPVAQNSKVEVIIQERRDTKPGVPHIREWDRGKDFSFGFWSKKQSELRAERDPEFAPPSNY 397
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Match: A0A2P6L3A1 (Coiled-coil domain-containing protein OS=Nephila clavipes OX=2585209 GN=NCL1_18572 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.779e-15
Identity = 82/284 (28.87%), Postives = 126/284 (44.37%), Query Frame = 3 
Query:  102 NVSSLITLKSDLVK-----RKAEARSNAESKKSLDLYSFNKISRKSDK----VPITCKKTIDITQKSNDLEFKNSITNLERKSQLYDKLIDNTSYDNDFLVDFSXXXXXXXXXXXXNFYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVD---VDHQDLLPKLQPAKDSEDTSID-TSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTN-------------FAPPAFY 875
            N SS+I LK++L K     R+A+   +    K     S NK  +  DK    VP+  +K  D      +L  K S   LE K++LY+K++ N        ++FS                     E+RDHG  YF FS + +KRKEQMEEL   + E   ++    K+Q      L++RL KI  + N+D   +D   ++P+ +     E  ++D +S+P+       E     K    R WD GK ++   + +K RK +  N             FAPP+ Y
Sbjct:    9 NPSSIIDLKAELHKKYDSFRRAKLDKSTNLSKRTPFISKNKSVQNKDKKQKPVPVVKQKVED---SEEELALKKSREALEAKAKLYEKIVSNKILIAILTIEFS---------------------EVRDHGVAYFAFSENEAKRKEQMEELNTLRQETESQRIARQKLQMKRKTMLQTRLAKICERKNIDPSALDEYKIVPETENVNTCEAPTLDLSSIPLP----EHEQEVPKKQPKIRPWDIGKDSLQHFVPEKKRKIKSQNDYVEERREERLSEFAPPSMY 264
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Match: A0A2C9LCF6 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106064561 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.487e-12
Identity = 51/162 (31.48%), Postives = 83/162 (51.23%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKL-------------QPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y+ +   EIR+HG G+F+FS D  +R+ ++  L+K   E I E++  + ++     ++E RL K++ + N+    QD+  K              QP      TS +  VP V  D+       TK K  REWDRGK    ++  ++ RK+R+++FAPP  Y
Sbjct:  117 YSNIQFDEIRNHGVGFFQFSKDHEQRENELASLKKLHKETIDEQARKDSIKAKRKTQMEERLAKVRQRRNLKEVKQDIDAKANTQLIISDSDSCKQPVSAELSTSKELDVPSVHQDV--STSEQTK-KPIREWDRGKVFPVKSYVEERRKERESDFAPPDSY 275
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Match: A0A2C9LBZ6 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106064561 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.792e-12
Identity = 51/162 (31.48%), Postives = 83/162 (51.23%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKL-------------QPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y+ +   EIR+HG G+F+FS D  +R+ ++  L+K   E I E++  + ++     ++E RL K++ + N+    QD+  K              QP      TS +  VP V  D+       TK K  REWDRGK    ++  ++ RK+R+++FAPP  Y
Sbjct:  199 YSNIQFDEIRNHGVGFFQFSKDHEQRENELASLKKLHKETIDEQARKDSIKAKRKTQMEERLAKVRQRRNLKEVKQDIDAKANTQLIISDSDSCKQPVSAELSTSKELDVPSVHQDV--STSEQTK-KPIREWDRGKVFPVKSYVEERRKERESDFAPPDSY 357
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Match: A0A4W6EKW7 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=ccdc174 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.758e-11
Identity = 51/169 (30.18%), Postives = 84/169 (49.70%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKT----KYNVDVDHQ--------------DLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSK--REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y  +   E R+ G GYF FS D  +R++Q + L   +D+  +++S   +++E     L++RL K++     K  +D   +              DL+    P +D+ + SI   V V I +     RR+TK      REWDRGK+ +F   + + R +RD+ FAPP+ Y
Sbjct:  227 YEDIRGQEARELGVGYFAFSHDEEQRRKQRDTLDMLRDQTTEQRSKRERLKEKRQAMLQARLAKVRQRKMKKSKLDGTEEEQREEENEGQEEEDDLIGSPPPPEDAPEVSIVKKVEVEIQE-----RRDTKPGVPHVREWDRGKEFMFSEWKTRCRDERDSEFAPPSAY 390
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Match: A0A3Q3XCV1 (Coiled-coil domain containing 174 OS=Mola mola OX=94237 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.453e-11
Identity = 50/157 (31.85%), Postives = 80/157 (50.96%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKI------KTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSK--REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y  +   E RD G GYF FS D  +R++Q E L   +D+  ++++   +++      L++RL K+      K K +      +L+    P +D  + SI   V V I +     RR+TK      REWDRGK+ +F   + + R++RD+ FAPP  Y
Sbjct:  224 YEDIRGQEARDLGVGYFAFSQDQEQRRKQRETLDMLRDQTTEQRTKREQLKGKRQAILKARLAKVRQRKMKKAKLDGAEGEDELIGHPPPLEDIPEVSIVRKVEVEIQE-----RRDTKPGVPHVREWDRGKEFMFSEWKTRRREERDSEFAPPTQY 375
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Cavefish
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:103028582])

HSP 1 Score: 60.077 bits (144), Expect = 9.632e-10
Identity = 44/162 (27.16%), Postives = 77/162 (47.53%), Query Frame = 3 
Query:  426 FYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKI------KTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVP--VVIDDLLFEIRRNTKSKSK----REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
             Y  + + E R+ G GYF F+ D  +RK+Q E L   +D+  ++++   +++E     LE+RL K+      K+K     +      +     + E   +    P   V+  +  EI+    S+      REWDRGK+  +     + R++RD++FAPP  Y
Sbjct:  226 HYENIRDQEARELGVGYFAFAHDEQQRKKQRETLDMLRDQTTEQRNKREQLKEKRKALLEARLAKVRMRKMKKSKLESGDESAAAPEEDVDKDEDEAEDVIGPSPSETVVKKVEVEIQERKDSRPGVPHVREWDRGKEFSWGQWTSRRREERDSDFAPPTSY 387
[Close]
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Sea Lamprey
Match: ccdc174 (coiled-coil domain containing 174 [Source:ZFIN;Acc:ZDB-GENE-050320-54])

HSP 1 Score: 73.9442 bits (180), Expect = 5.087e-15
Identity = 53/170 (31.18%), Postives = 84/170 (49.41%), Query Frame = 3 
Query:  426 FYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKT----KYNVD------VDHQDLLPKLQPAKDSEDTSIDTS-VPVVIDDLLFEIRRNTKSKSK---REWDRGKKTIFENIQK------KLRKDRDTNFAPPAFY 875
             Y  +   E+RD G GYF FS D  KRK QM+ L   +D+ + ++S   K++E     LE+RL++++     K   D      +D + +    +PA+D          V VV+     ++RR+TK       R+WD+GK  + ++ QK      +L +DR   FAPP  Y
Sbjct:  248 HYEHLREHEVRDLGVGYFAFSKDELKRKRQMDTLDMLRDQTLDQRSKREKLKEKRQAVLEARLERVRQRKGKKPGTDGPEGETLDGEHVRAGSEPAEDVLQPGPSAGKVEVVV-----QVRRDTKKGEMPRVRDWDKGKDFMLQDWQKGHGSQQRLPRDRPAEFAPPTTY 412
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Nematostella
Match: EDO42063 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2L9])

HSP 1 Score: 48.1358 bits (113), Expect = 2.291e-7
Identity = 18/35 (51.43%), Postives = 28/35 (80.00%), Query Frame = 3 
Query:  444 NTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEI 548
            + E+R HG GY++FS+D ++R +QME LRK +DE+
Sbjct:   76 DAEVRAHGVGYYQFSTDETERSKQMETLRKLRDEV 110
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Medaka
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:101171903])

HSP 1 Score: 67.3958 bits (163), Expect = 4.312e-12
Identity = 48/164 (29.27%), Postives = 79/164 (48.17%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKT----KYNVD---------VDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSK--REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y  +   E R+ G GYF FS D  +R++Q E L   +D+   +++   +++E     L++RL K++     K  +D         V+ ++         D  + S    V V I +     RR+TK      REWDRGK+ +F   + + R +R+T FAPP+ Y
Sbjct:  228 YEDIRAQEARELGVGYFAFSQDQEQRRKQRETLDMLRDQTTDQRTKRERLKEKRQAILKARLAKVRQRKMKKAKLDGTEEESKDTVEEEETFGPFPQPDDPPEVSTLKRVEVEIQE-----RRDTKPGVPHIREWDRGKEFMFSEWKSRRRDERETEFAPPSAY 386
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Medaka
Match: ccdc174 (coiled-coil domain containing 174 [Source:NCBI gene;Acc:101171903])

HSP 1 Score: 67.3958 bits (163), Expect = 4.391e-12
Identity = 48/164 (29.27%), Postives = 79/164 (48.17%), Query Frame = 3 
Query:  429 YTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKT----KYNVD---------VDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSK--REWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            Y  +   E R+ G GYF FS D  +R++Q E L   +D+   +++   +++E     L++RL K++     K  +D         V+ ++         D  + S    V V I +     RR+TK      REWDRGK+ +F   + + R +R+T FAPP+ Y
Sbjct:  228 YEDIRAQEARELGVGYFAFSQDQEQRRKQRETLDMLRDQTTDQRTKRERLKEKRQAILKARLAKVRQRKMKKAKLDGTEEESKDTVEEEETFGPFPQPDDPPEVSTLKRVEVEIQE-----RRDTKPGVPHIREWDRGKEFMFSEWKSRRRDERETEFAPPSAY 386
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BLAST of Coiled-coil domain-containing protein 174 vs. Planmine SMEST
Match: SMESG000018130.1 (SMESG000018130.1)

HSP 1 Score: 485.723 bits (1249), Expect = 2.985e-175
Identity = 258/260 (99.23%), Postives = 258/260 (99.23%), Query Frame = 3 
Query:   96 MANVSSLITLKSDLVKRKAEARSNAESKKSLDLYSFNKISRKSDKVPITCKKTIDITQKSNDLEFKNSITNLERKSQLYDKLIDNTSYDNDFLVDFSXXXXXXXXXXXXNFYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 875
            MANVSSLITLKSDLVKRKAEARSNAESKKSLDLYSFNKI RKSDKVPITCKKTIDIT KSNDLEFKNSITNLERKSQLYDKLIDNTSYDNDFLVDFSKKDKLLIEEDEENFYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY
Sbjct:    1 MANVSSLITLKSDLVKRKAEARSNAESKKSLDLYSFNKIPRKSDKVPITCKKTIDITHKSNDLEFKNSITNLERKSQLYDKLIDNTSYDNDFLVDFSKKDKLLIEEDEENFYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDEIIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSEDTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDRDTNFAPPAFY 260
The following BLAST results are available for this feature:
BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CCDC1742.953e-1028.99coiled-coil domain containing 174 [Source:HGNC Sym... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C37A2.83.047e-729.37pep chromosome:WBcel235:I:6798232:6799674:-1 gene:... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1742.017e-1327.78coiled-coil domain containing 174 [Source:ZFIN;Acc... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ccdc1741.720e-1028.48coiled-coil domain containing 174 [Source:NCBI gen... [more]
ccdc1742.040e-928.83coiled-coil domain containing 174 [Source:NCBI gen... [more]
ccdc1743.860e-927.50coiled-coil domain containing 174 [Source:NCBI gen... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ccdc1741.893e-828.24coiled-coil domain containing 174 [Source:MGI Symb... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6PII3|CC174_HUMAN1.418e-928.99Coiled-coil domain-containing protein 174 OS=Homo ... [more]
sp|Q5PQS7|CC174_RAT2.657e-929.81Coiled-coil domain-containing protein 174 OS=Rattu... [more]
sp|Q5ZIH9|CC174_CHICK8.835e-929.09Coiled-coil domain-containing protein 174 OS=Gallu... [more]
sp|Q3U155|CC174_MOUSE1.232e-728.24Coiled-coil domain-containing protein 174 OS=Mus m... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2P6L3A12.779e-1528.87Coiled-coil domain-containing protein OS=Nephila c... [more]
A0A2C9LCF66.487e-1231.48Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9LBZ66.792e-1231.48Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A4W6EKW72.758e-1130.18Uncharacterized protein OS=Lates calcarifer OX=818... [more]
A0A3Q3XCV13.453e-1131.85Coiled-coil domain containing 174 OS=Mola mola OX=... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1749.632e-1027.16coiled-coil domain containing 174 [Source:NCBI gen... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1745.087e-1531.18coiled-coil domain containing 174 [Source:ZFIN;Acc... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO420632.291e-751.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ccdc1744.312e-1229.27coiled-coil domain containing 174 [Source:NCBI gen... [more]
ccdc1744.391e-1229.27coiled-coil domain containing 174 [Source:NCBI gen... [more]
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BLAST of Coiled-coil domain-containing protein 174 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000018130.12.985e-17599.23SMESG000018130.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032505 ID=SMED30032505|Name=Coiled-coil domain-containing protein 174|organism=Schmidtea mediterranea sexual|type=transcript|length=941bp
TTTAAATTGTAATTTTAGGCATTATTAACAGTATTAATTGTTGAAATATT
CTCTCAGTTAATATTTATTTACAATTAAAGCAATTATTCTAACAAATGGC
TAATGTATCAAGTCTTATTACTCTCAAATCAGATTTAGTTAAGAGGAAAG
CTGAGGCTCGTTCTAATGCTGAATCCAAAAAATCTTTAGACCTATATTCA
TTTAACAAAATATCTCGAAAATCCGATAAAGTTCCAATTACATGTAAAAA
GACAATTGATATAACTCAAAAATCAAATGATTTGGAATTTAAGAATTCAA
TTACGAATTTAGAAAGGAAGTCTCAATTATACGATAAATTAATTGATAAT
ACTTCATATGATAATGATTTTTTGGTAGACTTTTCTAAAAAGGATAAATT
ATTGATTGAAGAAGATGAAGAAAATTTTTATACTAAAGTTCTTAACACGG
AAATTCGTGATCATGGTACTGGATATTTTAAATTTTCTTCTGATGTTTCC
AAAAGAAAAGAACAGATGGAAGAATTACGAAAATTTCAAGATGAAATTAT
CAAAGAAAAATCAAATCTTAATAAAGTCCAAGAAATAGAAAATCTGAAAC
TTGAATCCAGATTACAAAAAATTAAAACTAAGTATAATGTTGATGTTGAT
CATCAAGATCTTTTACCAAAACTTCAACCCGCTAAAGATTCAGAAGATAC
TTCAATTGATACTAGTGTACCTGTTGTTATTGACGATTTATTATTTGAAA
TAAGAAGAAACACTAAAAGTAAATCTAAAAGAGAATGGGATCGTGGGAAA
AAAACAATTTTCGAAAATATACAGAAAAAATTAAGGAAAGATCGTGATAC
TAATTTTGCTCCTCCCGCATTTTATTGAAAATATACCGTTTTGTTTATGA
ATTATCATTGAGTAATAATTATATAAAAATCTAAGTTATTT
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protein sequence of SMED30032505-orf-1

>SMED30032505-orf-1 ID=SMED30032505-orf-1|Name=SMED30032505-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=261bp
MANVSSLITLKSDLVKRKAEARSNAESKKSLDLYSFNKISRKSDKVPITC
KKTIDITQKSNDLEFKNSITNLERKSQLYDKLIDNTSYDNDFLVDFSKKD
KLLIEEDEENFYTKVLNTEIRDHGTGYFKFSSDVSKRKEQMEELRKFQDE
IIKEKSNLNKVQEIENLKLESRLQKIKTKYNVDVDHQDLLPKLQPAKDSE
DTSIDTSVPVVIDDLLFEIRRNTKSKSKREWDRGKKTIFENIQKKLRKDR
DTNFAPPAFY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR025066DUF4078
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 137..179
NoneNo IPR availablePANTHERPTHR15885UNCHARACTERIZEDcoord: 3..101
coord: 108..258
IPR025066Protein of unknown function DUF4078PFAMPF13300DUF4078coord: 104..179
e-value: 3.2E-14
score: 53.0