C2H2-type domain-containing protein

Overview
NameC2H2-type domain-containing protein
Smed IDSMED30032499
Length (bp)2380
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of C2H2-type domain-containing protein (SMED30032499) t-SNE clustered cells

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30032499) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of C2H2-type domain-containing protein (SMED30032499) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30032499) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032499

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30032499h1SMcG0014805 SmedASXL_011981SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Category 2 cellSMED30032499h1SMcG0014805 SMED30032499smed_20140614PMID:29100657
Cheng et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
epidermisSMED30032499h1SMcG0014805 SMED30032499smed_20140614PMID:29100657
Cheng et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Category 3 cellSMED30032499h1SMcG0014805 SMED30032499smed_20140614PMID:29100657
Cheng et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
neoblastSMED30032499h1SMcG0014805 SMED30032499smed_20140614PMID:29100657
Cheng et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
X1 cellSMED30032499h1SMcG0014805 SMED30032499smed_20140614PMID:29100657
Cheng et al., 2018		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30032499h1SMcG0014805 dd_Smed_v4_7464_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30032499h1SMcG0014805 dd_Smed_v4_5938_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A158QHT9 (C2H2-type domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS8049 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 4.324e-38
Identity = 120/348 (34.48%), Postives = 177/348 (50.86%), Query Frame = 3 
Query:  690 IALPYSTRVLSKL--SSGFDERLREDLTCKMAQYSKFKVRLEIVDGQK--QFVCSTCQYRSVHS-----------LADVRKHILGCHCSVSTSYTRLCLRASRL-DTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDT-SAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLER--------------NEK----IDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            + LPYS  VL KL  ++G   +   ++  KM  YS  +    I  G+K   F CS C+   V S           LAD R+H++G H  +S  +  +C +ASR+   N F LF+D  L   A   + R       DA+ D  S+ Q    +     +       P++ +A  S      ++  +  R S    +   S V   G   E               NE+    ++R+I L YS   LE LV   CPE Y  +L++KM +Y  +++ + RRN +RYF CS C + SPHGM DIRKHILGVHA++PER+KAAAM+CSR++R++  LLSDD LL+  +  ++W+
Sbjct:  862 LPLPYSKNVLRKLLENAGATPKQILEINEKMEVYS-LRTMKRICAGEKTLAFRCS-CKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEMLFRLA---MDRPIKLNTTDASSDKLSSHQTPRTASSRSSTSRLGSLPPLRPLADISAENSEEEKTAVNNRFSLPTVLHDSSTVSNGGLDDEDSGESIDMSRVLSCLNEQDPGSVERIIDLPYSRTALESLVHDYCPENYFLVLKKKMQIYTKYKVFVQRRNKRRYFCCSGCSSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNSLLSDDNLLQLAK--MKWK 1202

HSP 2 Score: 130.568 bits (327), Expect = 3.937e-27
Identity = 117/351 (33.33%), Postives = 171/351 (48.72%), Query Frame = 3 
Query:  690 IALPYSTRVLSKLSSGFDE---RLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANE---KLKRAESGTGWDANGDTSAMQLLD------DSEPEQESPIDT-ISR----------PIK---RVAISSG-AVGS---IKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKI---DRVIH--LNYSWHVLERLV--AGQCPERYLPILQQKMDVYAL---------HRILLTRRNGKRYFLCSHCPNCS--PHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELL 1598
            I LPYS  VL +L    DE     R  +  KM  YS  K   E  DG ++  C  C+Y S H+LADVRKHI+G HC +ST + R CL+ASRLD + F L SD +LI  A++   K     +      N ++ A  L D         PE   P +  ISR          P+K   R  I  G AV S   + ++ +    S S +    S   ++ + ++ N  +   DR++   L YS +VL +L+  AG  P++ L I  +KM+VY+L          + L  R + KR F+ S  P+    P  + D R+H++GVHA++   F       SR+ R+N   L +DE+L
Sbjct:  628 IPLPYSITVLERLLVQDDEVTPEQRVSIMEKMKIYSTMKAITETKDGVRRHKCCQCKYLSPHALADVRKHIMGSHCGISTKHFRHCLQASRLDNSDFGLLSDDRLIRMAHDSRSKKHENNTNNVNPENDESEAGSLADVRLNHSTPSPESREPDENGISRFTTMIPGSKTPVKVRIRGPIPPGEAVASSTPLTQSSIFINPSTSNEFIDGSLTIDLAELIKENPALAGADRIVDLPLPYSKNVLRKLLENAGATPKQILEI-NEKMEVYSLRTMKRICAGEKTLAFRCSCKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEML 977

HSP 3 Score: 70.8626 bits (172), Expect = 1.220e-8
Identity = 44/119 (36.97%), Postives = 58/119 (48.74%), Query Frame = 3 
Query:  669 PGDDCQMIALPYSTRVLSKLSSGF-DERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLK 1022
            PG   ++I LPYS   L  L   +  E     L  KM  Y+K+KV ++  + ++ F CS C   S H + D+RKHILG H  V   Y    +  SRL     SL SD  L+  A  K K
Sbjct: 1084 PGSVERIIDLPYSRTALESLVHDYCPENYFLVLKKKMQIYTKYKVFVQRRNKRRYFCCSGCSSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNSLLSDDNLLQLAKMKWK 1202
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A564Z2X3 (C2H2-type domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS11445 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 5.987e-38
Identity = 116/347 (33.43%), Postives = 176/347 (50.72%), Query Frame = 3 
Query:  690 IALPYSTRVLSKL--SSGFDERLREDLTCKMAQYSKFKVRLEIVDGQK--QFVCSTCQYRSVHS-----------LADVRKHILGCHCSVSTSYTRLCLRASRL-DTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEK------------------IDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            + LPYS  VL KL  ++G   +   ++  KM  YS  +    I  G K   F CS C+   V S           LAD R+H++G H  +S  +  +C +ASR+   N F LF+D  L   A ++  +  +     ++  +S       S     S + ++  P++ +A  S      +R+    R S    +         G   E +E+                  I+R+I L YS   L+ LV   CPE Y   L++KM +Y  +++ + RRN +RYF CS CP+ SPHGM DIRKHILGVHA++PER+KAAAM+CSR++R++  LLSDD LL+  +  ++W+
Sbjct:  847 LPLPYSKNVLRKLLENAGATPKQIVEINEKMEVYS-LRTMKRICAGDKTLAFRCS-CKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEMLFRLAMDRPIKLNTTDASPSDRQSSHQAPRTASSRSSNSRLGSLP-PLRPLADISADNSEEERSMANNRFSLPNVLHDSVTASNGGLDDEDSEEPIDMSRVLSCLNEQDPGSIERIIDLPYSKTALQSLVHDYCPENYFLELKKKMQIYTKYKVFVQRRNKRRYFCCSGCPSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLSDDNLLQLAK--MKWK 1188

HSP 2 Score: 127.102 bits (318), Expect = 5.381e-26
Identity = 106/352 (30.11%), Postives = 167/352 (47.44%), Query Frame = 3 
Query:  690 IALPYSTRVLSKLSSGFDE---RLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKR---------------AESGTGWDA------------NGDTSAMQLLDDSEPEQESPIDT-ISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKI---DRVIH--LNYSWHVLERLV--AGQCPERYLPILQQKMDVYALH---------RILLTRRNGKRYFLCSHCPNCS--PHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELL 1598
            I LPYS  VL +L    DE     R  +  KM  Y   K   E  DG ++  C  C+Y S H+LADVRKHI+G HC +ST + R CL+ASRLD + F L SD +LI  A++   +               +E+G+  D               D + +       P  ++P+   I  PI      + +  +I+ +      S S ++   S   ++ + ++ N  +   DR++   L YS +VL +L+  AG  P++ + I  +KM+VY+L          + L  R + KR F+ S  P+    P  + D R+H++GVHA++   F       SR+ R+N   L +DE+L
Sbjct:  613 IPLPYSIAVLERLLVQDDEVTPEQRVSIMEKMKIYCTMKAITETRDGVRRHKCCQCKYISPHALADVRKHIMGSHCGISTKHFRHCLQASRLDNSDFGLLSDDRLIRMAHDSRSKKHDNNVNNANLENDESEAGSLADVRLNHSTPSPESREPDENGISRFTTLIPGSKTPVKVRIRGPIPPGEAVTNSTPTIQSSTFLN-VSTSNEVNDSSSTIDLAELVKENPALIGADRIVDLPLPYSKNVLRKLLENAGATPKQIVEI-NEKMEVYSLRTMKRICAGDKTLAFRCSCKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEML 962

HSP 3 Score: 73.9442 bits (180), Expect = 1.478e-9
Identity = 40/112 (35.71%), Postives = 61/112 (54.46%), Query Frame = 3 
Query: 1287 IHLNYSWHVLERLVAGQ---CPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQD 1613
            I L YS  VLERL+       PE+ + I++ KM +Y   + +   R+G R   C  C   SPH + D+RKHI+G H  +  +     +  SR++  ++GLLSDD L++   D
Sbjct:  613 IPLPYSIAVLERLLVQDDEVTPEQRVSIME-KMKIYCTMKAITETRDGVRRHKCCQCKYISPHALADVRKHIMGSHCGISTKHFRHCLQASRLDNSDFGLLSDDRLIRMAHD 723

HSP 4 Score: 72.0182 bits (175), Expect = 6.073e-9
Identity = 43/119 (36.13%), Postives = 59/119 (49.58%), Query Frame = 3 
Query:  669 PGDDCQMIALPYSTRVLSKLSSGF-DERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLK 1022
            PG   ++I LPYS   L  L   +  E    +L  KM  Y+K+KV ++  + ++ F CS C   S H + D+RKHILG H  V   Y    +  SRL     +L SD  L+  A  K K
Sbjct: 1070 PGSIERIIDLPYSKTALQSLVHDYCPENYFLELKKKMQIYTKYKVFVQRRNKRRYFCCSGCPSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLSDDNLLQLAKMKWK 1188
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A158QCI3 (C2H2-type domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS1543 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 8.406e-38
Identity = 116/347 (33.43%), Postives = 176/347 (50.72%), Query Frame = 3 
Query:  690 IALPYSTRVLSKL--SSGFDERLREDLTCKMAQYSKFKVRLEIVDGQK--QFVCSTCQYRSVHS-----------LADVRKHILGCHCSVSTSYTRLCLRASRL-DTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEK------------------IDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            + LPYS  VL KL  ++G   +   ++  KM  YS  +    I  G K   F CS C+   V S           LAD R+H++G H  +S  +  +C +ASR+   N F LF+D  L   A ++  +  +     ++  +S       S     S + ++  P++ +A  S      +R+    R S    +         G   E +E+                  I+R+I L YS   L+ LV   CPE Y   L++KM +Y  +++ + RRN +RYF CS CP+ SPHGM DIRKHILGVHA++PER+KAAAM+CSR++R++  LLSDD LL+  +  ++W+
Sbjct:  978 LPLPYSKNVLRKLLENAGATPKQIVEINEKMEVYS-LRTMKRICAGDKTLAFRCS-CKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEMLFRLAMDRPIKLNTTDASPSDRQSSHQAPRTASSRSSNSRLGSLP-PLRPLADISADNSEEERSMANNRFSLPNVLHDSVTASNGGLDDEDSEEPIDMSRVLSCLNEQDPGSIERIIDLPYSKTALQSLVHDYCPENYFLELKKKMQIYTKYKVFVQRRNKRRYFCCSGCPSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLSDDNLLQLAK--MKWK 1319

HSP 2 Score: 126.716 bits (317), Expect = 6.340e-26
Identity = 106/352 (30.11%), Postives = 167/352 (47.44%), Query Frame = 3 
Query:  690 IALPYSTRVLSKLSSGFDE---RLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKR---------------AESGTGWDA------------NGDTSAMQLLDDSEPEQESPIDT-ISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKI---DRVIH--LNYSWHVLERLV--AGQCPERYLPILQQKMDVYALH---------RILLTRRNGKRYFLCSHCPNCS--PHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELL 1598
            I LPYS  VL +L    DE     R  +  KM  Y   K   E  DG ++  C  C+Y S H+LADVRKHI+G HC +ST + R CL+ASRLD + F L SD +LI  A++   +               +E+G+  D               D + +       P  ++P+   I  PI      + +  +I+ +      S S ++   S   ++ + ++ N  +   DR++   L YS +VL +L+  AG  P++ + I  +KM+VY+L          + L  R + KR F+ S  P+    P  + D R+H++GVHA++   F       SR+ R+N   L +DE+L
Sbjct:  744 IPLPYSIAVLERLLVQDDEVTPEQRVSIMEKMKIYCTMKAITETRDGVRRHKCCQCKYISPHALADVRKHIMGSHCGISTKHFRHCLQASRLDNSDFGLLSDDRLIRMAHDSRSKKHDNNVNNANLENDESEAGSLADVRLNHSTPSPESREPDENGISRFTTLIPGSKTPVKVRIRGPIPPGEAVTNSTPTIQSSTFLN-VSTSNEVNDSSSTIDLAELVKENPALIGADRIVDLPLPYSKNVLRKLLENAGATPKQIVEI-NEKMEVYSLRTMKRICAGDKTLAFRCSCKRLFVTSRQPDAKMRPATLADSRRHVMGVHAKISHEFITICCQASRICRENGFELFNDEML 1093

HSP 3 Score: 73.559 bits (179), Expect = 1.882e-9
Identity = 40/112 (35.71%), Postives = 61/112 (54.46%), Query Frame = 3 
Query: 1287 IHLNYSWHVLERLVAGQ---CPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQD 1613
            I L YS  VLERL+       PE+ + I++ KM +Y   + +   R+G R   C  C   SPH + D+RKHI+G H  +  +     +  SR++  ++GLLSDD L++   D
Sbjct:  744 IPLPYSIAVLERLLVQDDEVTPEQRVSIME-KMKIYCTMKAITETRDGVRRHKCCQCKYISPHALADVRKHIMGSHCGISTKHFRHCLQASRLDNSDFGLLSDDRLIRMAHD 854

HSP 4 Score: 71.633 bits (174), Expect = 6.802e-9
Identity = 43/119 (36.13%), Postives = 59/119 (49.58%), Query Frame = 3 
Query:  669 PGDDCQMIALPYSTRVLSKLSSGF-DERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLK 1022
            PG   ++I LPYS   L  L   +  E    +L  KM  Y+K+KV ++  + ++ F CS C   S H + D+RKHILG H  V   Y    +  SRL     +L SD  L+  A  K K
Sbjct: 1201 PGSIERIIDLPYSKTALQSLVHDYCPENYFLELKKKMQIYTKYKVFVQRRNKRRYFCCSGCPSISPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLSDDNLLQLAKMKWK 1319
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A2H1CRV7 (Zinc finger, C2H2 type OS=Fasciola hepatica OX=6192 GN=D915_03117 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 5.388e-36
Identity = 127/381 (33.33%), Postives = 184/381 (48.29%), Query Frame = 3 
Query:  672 GDDCQMIALPYSTRVLSKL--SSGFDERLREDLTCKMAQYSKFKV-RLEIVDGQKQFVC-------STCQYRS---VHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTN-TFSLFSDRKLISFANEKLKRAESGTGWDANGDTS-AMQLLDDSEPEQESPIDTIS------RPIKRVA-----------ISSGAVGSIKRAKLTQRQSHSA-----------------KMRPDS-----PVPEVGQPLER--------NEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
             D    + LP+S  VL KL  S+G  E    D+  KM  YS + + R+   D    F C       +T Q  S     +LAD R+H++G H  +S     +C +ASR+  +  F L+SD  L+  A E+  +  S     AN   S A   L  +      P +  +      RP+K +A            S  A   I+    T+   H+                     P+S     P   +  PL           E+++RVI+L YS   L  LV G CP  Y P+L++KMDVY+  ++ +TRR G+RYF CS C + SPHGM DIRKHILGVHA++PER+KAAAM+CSR++R++  LL +  LL   +  ++W+
Sbjct: 1357 ADRVVDLPLPFSEAVLRKLLDSAGATESQTRDILSKMQVYSTYTMTRICRGDRTLAFRCPCGRLFVTTRQPDSGIRAATLADSRRHVMGVHARISHDVITICCQASRITRDYDFELYSDEMLLRLAMERPIKLNSTALSSANNSASVASSTLGTTSASSSGPNNKGTSFHHTHRPLKELAPYPPILPNLVRKSVSADTGIESGTDTRDSVHTPNSVAHRDQNDDSNEDRDASDPESSGARKPQKGLEDPLSHLTKAQASTTEEVERVINLPYSSEALYSLVQGYCPASYFPVLEEKMDVYSSLKVFITRRRGRRYFCCSGCSSSSPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLPNHVLLHLAK--MKWK 1735

HSP 2 Score: 105.531 bits (262), Expect = 2.956e-19
Identity = 82/245 (33.47%), Postives = 112/245 (45.71%), Query Frame = 3 
Query:  363 DHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKR---AESGTG----------WDANG 1058
            +H  +P  CP C   F      L  FK+H L + H      ++K                   C++   L    E+  F+     LA  Q  P+        PG   + + LPYS  +L +L+       RE L  KM  YS+  V ++   G +++ C+ C Y S H+LADVRKHILG HC +ST + R CL+ASRLD   F+LFSD KL   A E L R    E+G G          +D NG
Sbjct: 1024 NHRRIPV-CPECGKVFPYG---LGDFKRHLLTV-HLEVPREYLK------------------ECLRFTYLPKSEEK--FQEVQEQLAMRQMRPRM-----LEPGR--RRVPLPYSIAILRRLTGQLPVATREFLEQKMQLYSRLSVIVDTRGGYRRYKCNHCTYSSPHALADVRKHILGSHCGISTKHFRHCLQASRLDPVEFTLFSDDKLARLAKEFLHRRQGLETGKGESRSPSPTGSYDQNG 1236

HSP 3 Score: 78.1814 bits (191), Expect = 8.427e-11
Identity = 40/116 (34.48%), Postives = 63/116 (54.31%), Query Frame = 3 
Query: 1281 RVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            R + L YS  +L RL  GQ P      L+QKM +Y+   +++  R G R + C+HC   SPH + D+RKHILG H  +  +     +  SR++   + L SDD+L +  ++ +  R
Sbjct: 1099 RRVPLPYSIAILRRLT-GQLPVATREFLEQKMQLYSRLSVIVDTRGGYRRYKCNHCTYSSPHALADVRKHILGSHCGISTKHFRHCLQASRLDPVEFTLFSDDKLARLAKEFLHRR 1213
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A4E0RHE7 (Zinc finger C2H2 OS=Fasciola hepatica OX=6192 GN=D915_001903 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 6.984e-36
Identity = 126/375 (33.60%), Postives = 183/375 (48.80%), Query Frame = 3 
Query:  690 IALPYSTRVLSKL--SSGFDERLREDLTCKMAQYSKFKV-RLEIVDGQKQFVC-------STCQYRS---VHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTN-TFSLFSDRKLISFANEKLKRAESGTGWDANGDTS-AMQLLDDSEPEQESPIDTIS------RPIKRVA-----------ISSGAVGSIKRAKLTQRQSHSA-----------------KMRPDS-----PVPEVGQPLER--------NEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            + LP+S  VL KL  S+G  E    D+  KM  YS + + R+   D    F C       +T Q  S     +LAD R+H++G H  +S     +C +ASR+  +  F L+SD  L+  A E+  +  S     AN   S A   L  +      P +  +      RP+K +A            S  A   I+    T+   H+                     P+S     P   +  PL           E+++RVI+L YS   L  LV G CP  Y P+L++KMDVY+  ++ +TRR G+RYF CS C + SPHGM DIRKHILGVHA++PER+KAAAM+CSR++R++  LL +  LL   +  ++W+
Sbjct: 1290 LPLPFSEAVLRKLLDSAGATESQTRDILSKMQVYSTYTMTRICRGDRTLAFRCPCGRLFVTTRQPDSGIRAATLADSRRHVMGVHARISHDVITICCQASRITRDYDFELYSDEMLLRLAMERPIKLNSTALSSANNSASVASSTLGTTSASSSGPNNKGTSFHHTHRPLKELAPYPPILPNLVRKSVSADTGIESGTDTRDSVHTPNSVAHRDQNDDSNEDRDASDPESSGARKPQKGLEDPLSHLTKAQASTTEEVERVINLPYSSEALYSLVQGYCPASYFPVLEEKMDVYSSLKVFITRRRGRRYFCCSGCSSSSPHGMGDIRKHILGVHAKVPERYKAAAMHCSRLSREDNTLLPNHVLLHLAK--MKWK 1662

HSP 2 Score: 105.531 bits (262), Expect = 3.032e-19
Identity = 82/245 (33.47%), Postives = 112/245 (45.71%), Query Frame = 3 
Query:  363 DHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKR---AESGTG----------WDANG 1058
            +H  +P  CP C   F      L  FK+H L + H      ++K                   C++   L    E+  F+     LA  Q  P+        PG   + + LPYS  +L +L+       RE L  KM  YS+  V ++   G +++ C+ C Y S H+LADVRKHILG HC +ST + R CL+ASRLD   F+LFSD KL   A E L R    E+G G          +D NG
Sbjct:  951 NHRRIPV-CPECGKVFPYG---LGDFKRHLLTV-HLEVPREYLK------------------ECLRFTYLPKSEEK--FQEVQEQLAMRQMRPRM-----LEPGR--RRVPLPYSIAILRRLTGQLPVATREFLEQKMQLYSRLSVIVDTRGGYRRYKCNHCTYSSPHALADVRKHILGSHCGISTKHFRHCLQASRLDPVEFTLFSDDKLARLAKEFLHRRQGLETGKGESRSPSPTGSYDQNG 1163

HSP 3 Score: 77.7962 bits (190), Expect = 1.018e-10
Identity = 40/116 (34.48%), Postives = 63/116 (54.31%), Query Frame = 3 
Query: 1281 RVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWR 1628
            R + L YS  +L RL  GQ P      L+QKM +Y+   +++  R G R + C+HC   SPH + D+RKHILG H  +  +     +  SR++   + L SDD+L +  ++ +  R
Sbjct: 1026 RRVPLPYSIAILRRLT-GQLPVATREFLEQKMQLYSRLSVIVDTRGGYRRYKCNHCTYSSPHALADVRKHILGSHCGISTKHFRHCLQASRLDPVEFTLFSDDKLARLAKEFLHRR 1140
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000075452.1 (SMESG000075452.1)

HSP 1 Score: 1369.37 bits (3543), Expect = 0.000e+0
Identity = 711/713 (99.72%), Postives = 711/713 (99.72%), Query Frame = 3 
Query:   39 MANSHKISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININSTIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWRSVPPSNGSDDNLMEPLLPLQSAATTTAVGYEDNHNSNSKSPLSQCHXXXXXXXXXXXXXXXIFLSGDNNGGETSPYSSSLATDMFPSQFLAQYDDNNNHGLQTFFSTIFHPNINNTIGNSDSNYTNSIPHKFXXXXXXXXXXXXXXXXXXXXPKLIDDNSPVAXXXXXXXXXXXGTKLLSNGR 2177
            MANSHKISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININSTIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSP  DDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWRSVPPSNGSDDNLMEPLLPLQSAATTTAVGYEDNHNSNSKSPLSQCHNVSSINNNNKSSSHNIFLSGDNNGGETSPYSSSLATDMFPSQFLAQYDDNNNHGLQTFFSTIFHPNINNTIGNSDSNYTNSIPHKFSGTTTNNISSNNNDASSESSPKLIDDNSPVASMMMMINMNSSGTKLLSNGR
Sbjct:    1 MANSHKISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININSTIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSPSVDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWRSVPPSNGSDDNLMEPLLPLQSAATTTAVGYEDNHNSNSKSPLSQCHNVSSINNNNKSSSHNIFLSGDNNGGETSPYSSSLATDMFPSQFLAQYDDNNNHGLQTFFSTIFHPNINNTIGNSDSNYTNSIPHKFSGTTTNNISSNNNDASSESSPKLIDDNSPVASMMMMINMNSSGTKLLSNGR 713
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000033448.1 (SMESG000033448.1)

HSP 1 Score: 158.303 bits (399), Expect = 1.847e-39
Identity = 105/355 (29.58%), Postives = 166/355 (46.76%), Query Frame = 3 
Query:  672 GDDCQMIALPYSTRVLSKLSSGFDERLRED----LTCKMAQYSKFKV-RLEIVDGQKQFVCSTCQY----------RSVHSLADVRKHILGCHCSVSTSYTRLCLRASRL-DTNTFSLFSDRKLISFANEKLKRAE------------SGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKR----------------------------AKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDN 1568
             D    + LP+S  VL  L     + + ED    L  KM  YS++ + RL   D    F C   +               +LAD R+H++G H  +  S   +C +ASR+   N F ++SD  L+  A+E+  + +            S    D   D +    +D  +         +S P+  +  +S +   + +                            + L   Q   + +  +S V  +      + KI+R+IHL YS   L+ L  G C   + P+LQ+KM++Y+ ++I + RR G+R F CS CP+ SPHGM D++KHILGVHAR+PERFKAAAM+CSR++R++
Sbjct:  671 ADSNHSLTLPFSESVLQSL---LQQLIPEDQIPGLIQKMKLYSEYSLKRLCKSDRTVAFQCPCGRVFHTARIPDLKMRAATLADARRHVMGVHAKIPQSLITVCCQASRICRENDFQIYSDDMLLQLASERQGKIQDSQSRSNQVADSSSKSQDLQADQTGTSSVDLGDENSAINDQDLSSPMPSICENSVSENCVSKTNCFLTANFSEEDLISTNKEETPSFNFVSDLAVEQQKYSAIAEESAVSRLAS---ESSKIERIIHLPYSLEALKCLTDGYCEPSFFPVLQEKMNIYSKYKIFIVRREGRRIFCCSGCPSSSPHGMGDVKKHILGVHARIPERFKAAAMHCSRLSRED 1019

HSP 2 Score: 131.724 bits (330), Expect = 4.338e-31
Identity = 114/422 (27.01%), Postives = 187/422 (44.31%), Query Frame = 3 
Query:  384 DCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGT-GWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGS---IKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDV---YALHRILLTRRNGK------RYFLCSHCPNCSPHG--MNDIRKHILGVHARLPERFKAAAMYCSRMNRDN-YGLLSDDELLK 1601
            +CP C   FS        FK+HFL + H   E  F+K                   C+K   L    ++     +   + +L+       KI  P         LPYS   L +L+    +  ++ L+ KM  YS   + ++  D  ++F+C+ C Y S ++LADVRKHI+G HC +ST + R CL+ASRLD N F+L SD K+++ A +  +R +  + G  ++   S    L   +     P ++   P+ +  +    V     +    L  R+  +A              L  N   +  + L +S  VL+ L+    PE  +P L QKM +   Y+L R+  + R         R F  +  P+       + D R+H++GVHA++P+         SR+ R+N + + SDD LL+
Sbjct:  418 ECPECHKVFSYG---FGDFKRHFLSV-HLDIEKEFIK------------------ECLKFTRLPKSNDKFQELQEQIIMRNLRPKSIDKDKIKVP---------LPYSIVALRQLTKFLPQSTQQYLSEKMRAYSTMSIIIQNYDNSRRFLCNQCTYSSPYALADVRKHIMGSHCGISTKHFRFCLQASRLDPNEFTLLSDDKILNLAEDLSRRTKHKSFGVSSHNRRSQRHALAKKKRAYSVP-NSKEPPVNKSPVKFEIVTQTPPVSNHSLGGRKGVNA--------------LYLNADSNHSLTLPFSESVLQSLLQQLIPEDQIPGLIQKMKLYSEYSLKRLCKSDRTVAFQCPCGRVFHTARIPDLKMRAATLADARRHVMGVHAKIPQSLITVCCQASRICRENDFQIYSDDMLLQ 793

HSP 3 Score: 66.2402 bits (160), Expect = 9.094e-11
Identity = 35/112 (31.25%), Postives = 59/112 (52.68%), Query Frame = 3 
Query: 1287 IHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVR 1622
            + L YS   L +L     P+     L +KM  Y+   I++   +  R FLC+ C   SP+ + D+RKHI+G H  +  +     +  SR++ + + LLSDD++L   +D+ R
Sbjct:  489 VPLPYSIVALRQLTKF-LPQSTQQYLSEKMRAYSTMSIIIQNYDNSRRFLCNQCTYSSPYALADVRKHIMGSHCGISTKHFRFCLQASRLDPNEFTLLSDDKILNLAEDLSR 599

HSP 4 Score: 59.3066 bits (142), Expect = 1.213e-8
Identity = 47/182 (25.82%), Postives = 79/182 (43.41%), Query Frame = 3 
Query:   57 ISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININSTIDHMELDHPDL----PFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMH-NGSRCMKVECLITCTE 587
            ++ +E+    F+C  C +N S  N + F         E H C       FEC+YC   + + N  +HI  HF       Y C  C     + +   + H++L H DL     + C  C+  F      L+H K + + +F+C  +NC  KS +   +  H ++ H   S   ++   +TC+E
Sbjct:   18 VTRIEESKGCFICVDCPNNFSFWNWEDF---------ESHSCVCEKKPYFECIYCGLLVLNKNGERHINMHFQHQCYNLYNCSLCTFSTNVEMQ-YLKHIKL-HNDLNSTLKYKCNSCQLDFDTVVAFLSHIKFYLMVMFNCCVKNCIAKSRSKQQMCEHYRKCHPTKSHNFEITQFLTCSE 188
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000062598.1 (SMESG000062598.1)

HSP 1 Score: 113.235 bits (282), Expect = 1.170e-25
Identity = 129/561 (22.99%), Postives = 229/561 (40.82%), Query Frame = 3 
Query:  210 CVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKIN-INSTIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLA------HMKRMH-----NGSRCMKVECL--------------------ITCTE-RISFKSQCNCL---------------------------ASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIK-RVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDR-VIHLNYSWHVLERLVAGQCPERYLPI--LQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELLKKNQDMVRWRSVPPSNGSDDNLMEPLLPLQSAAT 1700
            C  C   +P+ +++QH   H +        C  C   I  N I   ++HM   H ++  +CP C++++    DL  H +K +  I HC +++C  K+  M  +L       HMK++       G + +KV+ L                    I   E  +  KS  N L                           + L++S      +NS   +D + I   YS  V  K  +  +  + ED   K+++ S + + +   +  K F+C+ C+Y S HS  D+++H+   HC +S     L ++ +R D+ +  L  DR LI F N  +K            + SA +L+  S+ E    I T  +  K +  IS G +                K+  +SPV         N  I + + + +YS+ VL + +A   P+   PI    +K++  +   + +  RN  + + C +C   S H   DI++HI   H  +     ++ +  SR +  N  LLSD EL++   + +  + +       D+  E +L   +  T
Sbjct:   19 CSICSLDIPTSSMIQHSSVHLSHKGLSADNCLFCNEVISTNNIEDILEHMIYAH-EMKSNCPLCKSEYLTMDDLYHHLRKSYKVIHHCCYQDCTFKTDKMRELLKHKKQKRHMKKLTFTESVYGCKYVKVDVLSSDELDFPELPVEIKESKDVINEVELHLELKSVENQLNPRNSNSRLKRKSKIFDPSPEPIKRFKSDLRKSINLDFTLNS--NEDIKSIVFTYSETVFEKYVAVSNPTITEDFKTKLSKLSLYPMLIISDEKGKNFICNFCKYESRHSHTDIKRHVFRNHCDISNKNIPLMMKIARYDS-SMKLLPDRDLIRFINSDIKM----------NNLSAHRLISCSDDENVEKISTPQKNFKSKSRISKGDM---------------IKLINESPVT-------NNTTIYKDLFYFSYSYRVLMKYIAIAKPK---PIDNFVEKLEGLSKFPMSVCTRNKVKNYTCENCEYLSVHSHTDIKRHIFRNHCDILNANMSSILKVSRTS-SNMQLLSDSELIRIINNEISMKKIDADELLTDSDDESMLDWPAQKT 539

HSP 2 Score: 82.0333 bits (201), Expect = 7.577e-16
Identity = 82/326 (25.15%), Postives = 135/326 (41.41%), Query Frame = 3 
Query:  642 QSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEK--IDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCS---RMNRDNYGLLSDDELLKK 1604
            +SP+  N+    D  +    YS RVL K  +    +  ++   K+   SKF + +   +  K + C  C+Y SVHS  D+++HI   HC +  +     L+ SR  +N   L SD +LI   N ++    S    DA+      +LL DS+ E                                          D P  +  +  E++    I R I   YS ++L+ +   +    +   L+ KMDV++   +    RN  + ++C +C   S   ++++ +H L  HA L    K          +MN+D Y LLSDDE+ KK
Sbjct:  390 ESPVTNNTTIYKD--LFYFSYSYRVLMKYIAIAKPKPIDNFVEKLEGLSKFPMSVCTRNKVKNYTCENCEYLSVHSHTDIKRHIFRNHCDILNANMSSILKVSRTSSN-MQLLSDSELIRIINNEI----SMKKIDAD------ELLTDSDDES---------------------------------------MLDWPAQKTEKSFEKSRLNLISRKI-FKYSINILKAICYKENFLEHFERLKSKMDVFSTCGVETYYRNNSKIYMCLNCNWYSKISVDEVLRHALNYHAHLENETKFVKKIVETSYKMNKD-YCLLSDDEISKK 661
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000016820.1 (SMESG000016820.1)

HSP 1 Score: 100.523 bits (249), Expect = 1.738e-21
Identity = 91/341 (26.69%), Postives = 152/341 (44.57%), Query Frame = 3 
Query:  645 SPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDT-----ISRPIKRVAISSGAVGSIKR--AKLTQRQSHSAKMRP-DSPVPE----VGQPLERN-------EKID----RVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELL 1598
            S  ++N+P   D Q   L YS RV  KL     E+   +L  KM  ++KF +  + V  +  F+C  C Y++ HSL   RKHI   HC +   Y    ++AS       SL S++K+I   N   ++    +G       + ++LL DSE E+ S I           ++++ +S+G  G  K   A +         ++P DS  P     V Q +E N        ++D     V+ L YS  V  +L+     E +  I   KM +Y    +       K++++C+ C   + H  + IRKHI  +H  +P  +  A M  + M  + +  LS+ +++
Sbjct:  401 SESELNTPNASDIQ---LKYSERVFRKLLRNLPEKDFSNLNNKMKIFAKFTLTTKWVGEKMLFMCPKCSYKTTHSLDHTRKHIFRIHCKIPKIYLINSMQAS-------SLLSEKKMIRHINFYKQKMGKKSG-------AHVELLSDSEDEENSKISKSIVYGTPAKLRKIDLSNGISGISKNSIADIGTDVEPKVVLKPIDSCTPSKPSIVNQHVETNSLPSCSESELDTSNASVLQLTYSERVFRKLLRKLSEEDFSEI-HNKMKIYKECTLSTACVGDKKFYICNQCKYKTNHSPDRIRKHIFRMHCGIPSIYLTACMQANCMQSNFF--LSEKKMI 721

HSP 2 Score: 89.7373 bits (221), Expect = 3.637e-18
Identity = 75/307 (24.43%), Postives = 130/307 (42.35%), Query Frame = 3 
Query:  678 DCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDDSEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPVPEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYALHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAAMYCSRMNRDNYGLLSDDELL 1598
            +  ++ L YS RV  KL     E    ++  KM  Y +  +    V  +K ++C+ C+Y++ HS   +RKHI   HC + + Y   C++A+ + +N F   S++K+I   N + K+    +G +       ++LL DSE E++                    G +K                    P+  + +  +E     I L YS + L      +        L  KM  YA  + +   +N + Y +CS C   +   +    +H++  HA +P   +  A  CSR  +  Y LLSD+E+L
Sbjct:  617 NASVLQLTYSERVFRKLLRKLSEEDFSEIHNKMKIYKECTLSTACVGDKKFYICNQCKYKTNHSPDRIRKHIFRMHCGIPSIYLTACMQANCMQSNFF--LSEKKMIHNINFQRKKIAKKSGIN-------LELLSDSENEKD-------------------CGVLK--------------------PKNTRAVNLDE-----IKLGYSENALWYFCRKENQSTIFEDLNLKMQTYASIKGIFEYKNVRTYVICSACGYRTDKVIY-FSEHVMSNHAFVPVPLRELAKSCSRYKKFTYQLLSDEEML 869

HSP 3 Score: 68.5514 bits (166), Expect = 1.381e-11
Identity = 48/182 (26.37%), Postives = 77/182 (42.31%), Query Frame = 3 
Query:   54 KISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININSTID-HMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHN--GSRCMKVECLITCTER 590
            K+ +LE  +  +VC+ C           F+T+ P  FL+H    H  I    CVYCC ++     + H+  H +     ++ C  C +  K+    +I  H  + H D    CP C  +F+   D   H +     ++HC  + C V SHN    L H K  +       +  E +I+C  R
Sbjct:   10 KVQLLEN-SLKYVCTFC---------SKFSTNRPEEFLKHCAEFHEEIRMINCVYCCQNVIITQYVSHVDQHVSFMGRSKFFCLICNNSFKLTKTESITAHNSIFH-DHKNQCPVCLAEFAGTADFCNHIEGSVETLYHCGNKKCLVMSHNKDNFLQHFKNNYQICNKDSITEELVISCLNR 180
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000081028.1 (SMESG000081028.1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.264e-21
Identity = 89/323 (27.55%), Postives = 151/323 (46.75%), Query Frame = 3 
Query:   54 KISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCHPSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKINI-NSTIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCFVKSHNMIGVLAHMKRMHNGSRCMKV--ECLITCTE-RISFKSQCNCLASLQQSPQSPMKINSPPGDDCQMIALPYSTRVLSK-------LSSGFDERLREDLTCKMAQYSKFKVRLEIVDGQKQFV-CSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLCLRASRLDTNTFSLFSD 986
            +ISILE+    ++C  C           F T + ++F +H    H   ++  C+ C + L       H + H         +C  C    K++   S ++H+ L H ++   CP C  QF +  DL+ H    F+ IFHCSFENCFV S N+  ++ H K  H+     ++   CL    E R+ F      L +L    ++ + ++   G+      L Y  R+  +       ++SG D  L E L  K+    K+ + L   DG + F  CS C+Y +  S ++++KHIL  HC +S ++  +CL+ + L+   F++ S+
Sbjct:   14 RISILEQNTGKYICPTCV---------TFCTDSKAIFEDHLETNHKEFDKIVCICCSAVLDYSKYCSHFELHCQKDLMFHNICNFCNLYTKMDCRESVLEHLLLCHNNI-LCCPLCLIQFENELDLIKHVNNSFITIFHCSFENCFVVSTNINEIIFHKKTTHDYDLFDEIVFNCLPKIDENRLEFDQN---LENL-NCERNLLFLSGNSGNLSNKKYL-YVRRLKCRIKLFKEIINSGID--LYETLKKKLFNIDKYDI-LVCADGLEIFYKCSLCKYLTKKSHSNIKKHILLHHCEISIAHIEMCLKVTYLNNKKFNVNSE 318
The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A158QHT94.324e-3834.48C2H2-type domain-containing protein OS=Rodentolepi... [more]
A0A564Z2X35.987e-3833.43C2H2-type domain-containing protein OS=Hymenolepis... [more]
A0A158QCI38.406e-3833.43C2H2-type domain-containing protein OS=Hymenolepis... [more]
A0A2H1CRV75.388e-3633.33Zinc finger, C2H2 type OS=Fasciola hepatica OX=619... [more]
A0A4E0RHE76.984e-3633.60Zinc finger C2H2 OS=Fasciola hepatica OX=6192 GN=D... [more]
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BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000075452.10.000e+099.72SMESG000075452.1[more]
SMESG000033448.11.847e-3929.58SMESG000033448.1[more]
SMESG000062598.11.170e-2522.99SMESG000062598.1[more]
SMESG000016820.11.738e-2126.69SMESG000016820.1[more]
SMESG000081028.14.264e-2127.55SMESG000081028.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032499 ID=SMED30032499|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2380bp
CTATATAAATCTTATATGGTAGTTTAAAAATTGTGATAATGGCGAATTCT
CACAAAATTAGCATCCTTGAAAAAGTCAATCAAATGTTTGTCTGTTCAAT
TTGTTGTGATAATAAATCCGGATCCAATCCAAAACCATTTGCCACTTCCA
ATCCTTCGCTGTTTCTTGAGCACCATCGTTGTTGTCATCCATCAATCAAC
GAATTCGAGTGTGTTTATTGCTGTAGTCATTTGCCGAGTGTCAATTTAAT
GCAGCACATCCAAAACCATTTTACAGATTCGGCCAGTCCTCGATATGTAT
GTCCTCATTGCTGTTCACGAATCAAAATCAACATCAACAGCACGATAGAC
CACATGGAATTGGACCATCCAGATTTACCATTCGATTGCCCTCATTGTAG
AAATCAGTTTTCAGATCGAACAGATTTACTCACGCATTTCAAAAAGCATT
TTCTGTGTATTTTCCATTGTTCTTTCGAGAATTGTTTCGTCAAAAGTCAT
AACATGATTGGGGTGCTAGCACACATGAAACGTATGCATAACGGATCTCG
ATGCATGAAAGTGGAATGTCTGATTACATGTACGGAACGGATTTCATTCA
AATCTCAATGCAATTGCTTGGCAAGCTTGCAGCAGTCTCCCCAGTCACCA
ATGAAAATCAATAGTCCACCTGGGGACGATTGCCAAATGATAGCGTTGCC
TTATTCAACTCGGGTACTGAGCAAATTGTCATCGGGATTCGATGAGAGAT
TACGAGAAGATCTCACCTGTAAAATGGCTCAGTATTCCAAATTCAAAGTT
CGCCTGGAAATCGTCGATGGACAGAAACAGTTTGTCTGTTCCACATGCCA
GTATCGATCGGTGCATTCATTGGCAGATGTCCGCAAACACATCCTCGGAT
GTCACTGCAGTGTCAGCACGAGTTACACACGGCTTTGTTTGCGGGCCAGT
CGATTGGACACCAACACTTTCTCTCTGTTCTCTGATAGGAAATTGATTTC
ATTTGCCAATGAAAAACTGAAGCGAGCCGAATCCGGAACTGGCTGGGATG
CTAATGGAGACACGAGTGCAATGCAACTGCTTGATGACAGTGAGCCGGAG
CAGGAAAGCCCAATCGACACAATCAGTCGACCAATCAAACGGGTGGCAAT
TTCCAGTGGAGCTGTAGGCAGTATTAAGCGGGCAAAGCTTACTCAGCGAC
AGAGCCACTCGGCGAAAATGCGACCGGATTCCCCAGTTCCAGAGGTCGGT
CAGCCGCTGGAACGAAACGAAAAAATCGATCGTGTCATCCATTTGAACTA
CTCCTGGCATGTTCTAGAGCGCCTGGTAGCGGGCCAGTGTCCGGAACGGT
ACCTACCGATTTTGCAACAAAAGATGGATGTGTATGCGCTCCACCGGATC
CTGCTGACCCGTCGCAACGGCAAACGATATTTCCTGTGCTCTCATTGCCC
AAACTGCTCACCGCATGGAATGAATGATATCCGCAAACACATCCTCGGTG
TCCATGCCCGTCTCCCGGAACGGTTCAAGGCCGCTGCCATGTATTGCTCC
CGGATGAATCGGGACAACTATGGGCTGCTGTCAGATGATGAGCTGCTGAA
GAAAAACCAGGATATGGTTCGGTGGCGGTCTGTGCCCCCATCCAACGGTA
GTGATGACAATTTAATGGAACCGTTGCTACCCCTTCAATCAGCAGCCACA
ACAACGGCTGTGGGTTACGAGGACAACCACAACAGCAACAGTAAATCCCC
TCTTTCACAATGTCACAATGTTAGCAGTATTAATAACAATAATAAATCGT
CATCACACAACATCTTTCTGTCTGGTGATAACAACGGTGGTGAAACCAGT
CCCTATTCGTCGTCATTAGCAACCGATATGTTTCCCAGTCAATTCCTGGC
CCAGTATGATGACAACAACAATCATGGATTACAGACTTTCTTTTCCACCA
TTTTCCATCCAAACATTAATAACACCATTGGCAACAGTGATAGCAATTAC
ACCAATTCAATCCCACACAAATTCTCAGGTACGACGACGAATAACATCAG
CAGCAACAACAATGATGCTTCCAGTGAATCATCTCCGAAACTAATCGATG
ACAACTCGCCGGTTGCGTCGATGATGATGATGATCAATATGAACTCCAGC
GGTACAAAGCTGCTTAGCAACGGTCGCAGCAGAAGATCTCTAGCACTCAA
ATCTAAGAGCGCCGCTAGCACTGCAAATAACAACAGCAGTTAGAACAATT
ACACTCACTACGTTGAGCAGCAGCAGCAATACGACTATCTAACAACTTAC
AAGCGTTGATTGAGTCTGTGCCTGTATATTTTCATTATTTTTATTATTTT
CATTATAATATATTATTATTATTATTATTA
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protein sequence of SMED30032499-orf-1

>SMED30032499-orf-1 ID=SMED30032499-orf-1|Name=SMED30032499-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=735bp
MANSHKISILEKVNQMFVCSICCDNKSGSNPKPFATSNPSLFLEHHRCCH
PSINEFECVYCCSHLPSVNLMQHIQNHFTDSASPRYVCPHCCSRIKININ
STIDHMELDHPDLPFDCPHCRNQFSDRTDLLTHFKKHFLCIFHCSFENCF
VKSHNMIGVLAHMKRMHNGSRCMKVECLITCTERISFKSQCNCLASLQQS
PQSPMKINSPPGDDCQMIALPYSTRVLSKLSSGFDERLREDLTCKMAQYS
KFKVRLEIVDGQKQFVCSTCQYRSVHSLADVRKHILGCHCSVSTSYTRLC
LRASRLDTNTFSLFSDRKLISFANEKLKRAESGTGWDANGDTSAMQLLDD
SEPEQESPIDTISRPIKRVAISSGAVGSIKRAKLTQRQSHSAKMRPDSPV
PEVGQPLERNEKIDRVIHLNYSWHVLERLVAGQCPERYLPILQQKMDVYA
LHRILLTRRNGKRYFLCSHCPNCSPHGMNDIRKHILGVHARLPERFKAAA
MYCSRMNRDNYGLLSDDELLKKNQDMVRWRSVPPSNGSDDNLMEPLLPLQ
SAATTTAVGYEDNHNSNSKSPLSQCHNVSSINNNNKSSSHNIFLSGDNNG
GETSPYSSSLATDMFPSQFLAQYDDNNNHGLQTFFSTIFHPNINNTIGNS
DSNYTNSIPHKFSGTTTNNISSNNNDASSESSPKLIDDNSPVASMMMMIN
MNSSGTKLLSNGRSRRSLALKSKSAASTANNNSS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000028Category 2 cell
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR015880Znf_C2H2-like
IPR013087Znf_C2H2_type
IPR007087Znf_C2H2
IPR014023Mononeg_RNA_pol_cat
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
GO:0005524ATP binding
GO:0004482mRNA (guanine-N7-)-methyltransferase activity
GO:0003968RNA-directed 5'-3' RNA polymerase activity
Vocabulary: biological process
TermDefinition
GO:00063707-methylguanosine mRNA capping
Vocabulary: cellular component
TermDefinition
GO:0019012virion
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 23..56
e-value: 60.0
score: 6.4
coord: 271..295
e-value: 15.0
score: 11.8
coord: 471..495
e-value: 72.0
score: 5.7
coord: 121..143
e-value: 0.03
score: 23.5
coord: 148..173
e-value: 9.2
score: 13.6
coord: 92..116
e-value: 200.0
score: 1.8
coord: 62..83
e-value: 44.0
score: 7.6
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 123..143
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 121..143
score: 9.806
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 81..145
e-value: 3.1E-6
score: 29.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 717..740
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 388..409
NoneNo IPR availablePANTHERPTHR24409FAMILY NOT NAMEDcoord: 35..295