E3 ubiquitin-protein ligase

Overview


Name	E3 ubiquitin-protein ligase
Smed ID	SMED30032411
Length (bp)	2849

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of E3 ubiquitin-protein ligase (SMED30032411) t-SNE clustered cells

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase (SMED30032411) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of E3 ubiquitin-protein ligase (SMED30032411) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase (SMED30032411) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032411

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
protonephridia	SMED30032411	SMESG000030063.1	dd_Smed_v4_9145_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
posterior side	SMED30032411	SMESG000030063.1	dd_Smed_v4_9145_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
posterior muscle cell	SMED30032411	SMESG000030063.1	dd_Smed_v4_9145_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30032411	SMESG000030063.1	HO007253.1	ncbi_smed_ests	PMID:25956527 Henderson et al., 2015
parenchymal cell	SMED30032411	SMESG000030063.1	HO007253.1	ncbi_smed_ests	PMID:25956527 Henderson et al., 2015

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: SMURF1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:16807])

HSP 1 Score: 424.476 bits (1090), Expect = 5.172e-135
Identity = 276/820 (33.66%), Postives = 424/820 (51.71%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGS---SRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSG-----AIHWYHPAVPKCL-----RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLR-ALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLK-ELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +   D             I   GS    RGL  EN       SG         E           P P   S G            + GG   FV++ S      A    +P V   L     R  G  S    LP G++   +  G+VYF+   +  + + DPR+   +   P G A  +               Y   ++   S+ R  N    +          L    E+ +  +     +  N R+    Q        I++ +  +   S+   +     L    L  +R  R           ++  KL+ LR +L  +  Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   +L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDR------------IGTGGSVVDCRGLL-ENEGTVYEDSGPGRPLSCFME----------EPAPYTDSTG----------AAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQS--PELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFL---------------YEFLLQGHTSEPRDLNSVNCDE------LGPLPPGWEVRSTVSGRIYFVDHNNRTT---QFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYER-----------DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 756

[Close]

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGS---SRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSG-----AIHWYHPAVPKCL-----RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLR-ALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLK-ELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +   D             I   GS    RGL  EN       SG         E           P P   S G            + GG   FV++ S      A    +P V   L     R  G  S    LP G++   +  G+VYF+   +  + + DPR+   +   P G A  +               Y   ++   S+ R  N    +          L    E+ +  +     +  N R+    Q        I++ +  +   S+   +     L    L  +R  R           ++  KL+ LR +L  +  Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   +L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDR------------IGTGGSVVDCRGLL-ENEGTVYEDSGPGRPLSCFME----------EPAPYTDSTG----------AAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQS--PELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFL---------------YEFLLQGHTSEPRDLNSVNCDE------LGPLPPGWEVRSTVSGRIYFVDHNNRTT---QFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYER-----------DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 756

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: SMURF1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:16807])

HSP 1 Score: 384.8 bits (987), Expect = 3.082e-120
Identity = 181/384 (47.14%), Postives = 266/384 (69.27%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   +L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  350 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730

HSP 2 Score: 124.79 bits (312), Expect = 1.327e-28
Identity = 93/309 (30.10%), Postives = 146/309 (47.25%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSM----------PQIIHINGSSRGLTA--------ENSQQSLRYSGN-------QSSQRNSKEHSFSNDENNSRPGPSQISHGDLL--LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +   D   I T  S+            +   +G  R L+          +S  +    GN          QR   +   + D   S   P    HG     LP  +E++ +  G+VYF+   +G   W+ P +P   R +   +C  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDR--IGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP---RDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL 313

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: SMURF1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:16807])

HSP 1 Score: 384.8 bits (987), Expect = 3.082e-120
Identity = 181/384 (47.14%), Postives = 266/384 (69.27%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   +L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  350 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730

HSP 2 Score: 124.79 bits (312), Expect = 1.327e-28
Identity = 93/309 (30.10%), Postives = 146/309 (47.25%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSM----------PQIIHINGSSRGLTA--------ENSQQSLRYSGN-------QSSQRNSKEHSFSNDENNSRPGPSQISHGDLL--LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +   D   I T  S+            +   +G  R L+          +S  +    GN          QR   +   + D   S   P    HG     LP  +E++ +  G+VYF+   +G   W+ P +P   R +   +C  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDR--IGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP---RDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL 313

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Match: SMURF2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:16809])

HSP 1 Score: 383.645 bits (984), Expect = 1.282e-119
Identity = 229/614 (37.30%), Postives = 334/614 (54.40%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQT-----NRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L  S  V +N     T I  +      +  T  +SS   +    + S    N  S  H  T         +R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPV + +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPL--SCFVDEN-----TPISGT------NGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747

HSP 2 Score: 110.538 bits (275), Expect = 3.850e-24
Identity = 87/325 (26.77%), Postives = 145/325 (44.62%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 322.013 bits (824), Expect = 1.330e-99
Identity = 160/358 (44.69%), Postives = 231/358 (64.53%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR+N+ E+SF+ I R    DLR+RL ++F  EEGLDYGGVAREW +LLS  + +  + LF   G+N+ +L +++P   VN DHL YF +IGR I MA+FHG +I + F   FYK +LNK+  L+D E++D  IYNSL+W++DNNID+ D+ L F  + E+ G+L+  +L E G  I VT+ +K +YIE  V++  +    +Q +    G   V P + +   +E E E+LL G+ ++ ++DWQ+NT  +H       + WFW+ V S  +++R +LLQFVTG+ R+P+ GF  L GS G   P+LF I+ +   N   LP++HTCFNRLDLPPY SY  L +KL+ AIE T GF 
Sbjct:  154 ITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSL-QINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTG---PQLFCIERVGKEN--WLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFG 505

[Close]

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR+N+ E+SF+ I R    DLR+RL ++F  EEGLDYGGVAREW +LLS  + +  + LF   G+N+ +L +++P   VN DHL YF +IGR I MA+FHG +I + F   FYK +LNK+  L+D E++D  IYNSL+W++DNNID+ D+ L F  + E+ G+L+  +L E G  I VT+ +K +YIE  V++  +    +Q +    G   V P + +   +E E E+LL G+ ++ ++DWQ+NT  +H       + WFW+ V S  +++R +LLQFVTG+ R+P+ GF  L GS G   P+LF I+ +   N   LP++HTCFNRLDLPPY SY  L +KL+ AIE T GF 
Sbjct:  154 ITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSL-QINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTG---PQLFCIERVGKEN--WLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFG 505

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 321.627 bits (823), Expect = 1.498e-99
Identity = 160/358 (44.69%), Postives = 231/358 (64.53%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR+N+ E+SF+ I R    DLR+RL ++F  EEGLDYGGVAREW +LLS  + +  + LF   G+N+ +L +++P   VN DHL YF +IGR I MA+FHG +I + F   FYK +LNK+  L+D E++D  IYNSL+W++DNNID+ D+ L F  + E+ G+L+  +L E G  I VT+ +K +YIE  V++  +    +Q +    G   V P + +   +E E E+LL G+ ++ ++DWQ+NT  +H       + WFW+ V S  +++R +LLQFVTG+ R+P+ GF  L GS G   P+LF I+ +   N   LP++HTCFNRLDLPPY SY  L +KL+ AIE T GF 
Sbjct:  152 ITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSL-QINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTG---PQLFCIERVGKEN--WLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFG 503

[Close]

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR+N+ E+SF+ I R    DLR+RL ++F  EEGLDYGGVAREW +LLS  + +  + LF   G+N+ +L +++P   VN DHL YF +IGR I MA+FHG +I + F   FYK +LNK+  L+D E++D  IYNSL+W++DNNID+ D+ L F  + E+ G+L+  +L E G  I VT+ +K +YIE  V++  +    +Q +    G   V P + +   +E E E+LL G+ ++ ++DWQ+NT  +H       + WFW+ V S  +++R +LLQFVTG+ R+P+ GF  L GS G   P+LF I+ +   N   LP++HTCFNRLDLPPY SY  L +KL+ AIE T GF 
Sbjct:  152 ITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSL-QINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTG---PQLFCIERVGKEN--WLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFG 503

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Match: wwp-1 (E3 ubiquitin-protein ligase [Source:UniProtKB/TrEMBL;Acc:V6CJJ8])

HSP 1 Score: 325.865 bits (834), Expect = 6.959e-98
Identity = 160/358 (44.69%), Postives = 231/358 (64.53%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR+N+ E+SF+ I R    DLR+RL ++F  EEGLDYGGVAREW +LLS  + +  + LF   G+N+ +L +++P   VN DHL YF +IGR I MA+FHG +I + F   FYK +LNK+  L+D E++D  IYNSL+W++DNNID+ D+ L F  + E+ G+L+  +L E G  I VT+ +K +YIE  V++  +    +Q +    G   V P + +   +E E E+LL G+ ++ ++DWQ+NT  +H       + WFW+ V S  +++R +LLQFVTG+ R+P+ GF  L GS G   P+LF I+ +   N   LP++HTCFNRLDLPPY SY  L +KL+ AIE T GF 
Sbjct:  439 ITVSRNNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREWFFLLSHEVLNPMYCLFMYAGNNNYSL-QINPASFVNPDHLKYFEYIGRFIAMALFHGKFIYSGFTMPFYKKMLNKKIVLKDIEQVDSEIYNSLMWIKDNNIDECDMELYFVADYELLGELKTYELKEGGTEIAVTEENKLEYIELLVEWRFNRGVEQQTKAFFTGFNSVFPLEWMQYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVTWFWQWVRSLDQEKRARLLQFVTGTCRVPVGGFSELMGSTG---PQLFCIERVGKEN--WLPRSHTCFNRLDLPPYRSYDQLVEKLSMAIEMTEGFG 790

HSP 2 Score: 56.9954 bits (136), Expect = 7.121e-8
Identity = 35/116 (30.17%), Postives = 55/116 (47.41%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAV---------------------PKCLRKIGDFSC---LGLLPPGWDSLQS-DSGRVYFIDHISRTTQYSDPRLLRALEA--PDGY 931
            LP  +E +    G+VY+VD+N+    W  P                        + L +  +FS    LG LP GW+  Q  ++ R+YF++H++RTTQ+ DPR     +   PDG+
Sbjct:  255 LPQGWEMRRDPRGRVYYVDHNTRTTTWQRPTADMLEAHEQWQSGRDQAMLQWEQRFLLQQNNFSADDPLGPLPEGWEKRQDPNTSRMYFVNHVNRTTQWEDPRTQGGSDQPLPDGW 370

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Smurf (gene:FBgn0029006 transcript:FBtr0086833)

HSP 1 Score: 386.341 bits (991), Expect = 8.358e-118
Identity = 183/379 (48.28%), Postives = 257/379 (67.81%), Query Frame = 2

Query: 1343 KLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            KLR LR++L+ +  Q   C +E SR+ I EES+R I +++  D+RKRL++KF  EEGLDYGGVAREWL+LLS  + + ++GLF+ + D D   L+++P   VN DHL YFHF+GR +G+A+FHG+ +D  F   FYK +LNK   L D E +D  ++ SL WM ++NI    +  TFS+E   FG L + +L   G  IPVT+ +K +Y++ YV+Y       +Q+  ++KG  E++P  +L   +E E E+++ GIS I + DW+ NTRLKHC      + WFW+VV SY+ + R +LLQFVTGSSR+PL GF  L+GS G+  P+LFTI +  +    +LPKAHTCFNR+DLPPY +Y+ L DKLT A+EETCGFAV
Sbjct:  684 KLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRD-DHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGI-IESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAV 1060

HSP 2 Score: 71.633 bits (174), Expect = 2.906e-12
Identity = 33/82 (40.24%), Postives = 50/82 (60.98%), Query Frame = 2

Query:  659 LLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL-RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            L LP  +E + +  G+VYF    +G   W+ P +P+    +      +G LP GW+  ++ SGRVYF+DH +RTTQ++DPRL
Sbjct:  513 LDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594

HSP 3 Score: 64.6994 bits (156), Expect = 3.375e-10
Identity = 58/233 (24.89%), Postives = 96/233 (41.20%), Query Frame = 2

Query:   92 VKLKINSAHKLIKRSLLKNPDPFA--QIIYNNHSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTA----DFIDRGERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL 772
            V++ I  A  L ++ L + PDPFA  Q+      + T   + + +P WN  + + L  I D + I +WNQR     K S FLG   + A         G +RL L + +    + VRG + + ++  D  S    L+   I+  +G  RG                 S+ +S E S                     LP  +E + +D G+VY+V++ + +  W  P  P  +
Sbjct:   15 VRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFL-GIGDAITITVWNQRKI--HKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSSGNPLA---IVGPSGDVRG----------------PSEDDSSEDS---------------------LPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVV 204

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Smurf (gene:FBgn0029006 transcript:FBtr0333168)

HSP 1 Score: 386.341 bits (991), Expect = 8.358e-118
Identity = 183/379 (48.28%), Postives = 257/379 (67.81%), Query Frame = 2

Query: 1343 KLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            KLR LR++L+ +  Q   C +E SR+ I EES+R I +++  D+RKRL++KF  EEGLDYGGVAREWL+LLS  + + ++GLF+ + D D   L+++P   VN DHL YFHF+GR +G+A+FHG+ +D  F   FYK +LNK   L D E +D  ++ SL WM ++NI    +  TFS+E   FG L + +L   G  IPVT+ +K +Y++ YV+Y       +Q+  ++KG  E++P  +L   +E E E+++ GIS I + DW+ NTRLKHC      + WFW+VV SY+ + R +LLQFVTGSSR+PL GF  L+GS G+  P+LFTI +  +    +LPKAHTCFNR+DLPPY +Y+ L DKLT A+EETCGFAV
Sbjct:  684 KLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRD-DHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGI-IESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAV 1060

HSP 2 Score: 71.633 bits (174), Expect = 2.906e-12
Identity = 33/82 (40.24%), Postives = 50/82 (60.98%), Query Frame = 2

Query:  659 LLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL-RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            L LP  +E + +  G+VYF    +G   W+ P +P+    +      +G LP GW+  ++ SGRVYF+DH +RTTQ++DPRL
Sbjct:  513 LDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594

HSP 3 Score: 64.6994 bits (156), Expect = 3.375e-10
Identity = 58/233 (24.89%), Postives = 96/233 (41.20%), Query Frame = 2

Query:   92 VKLKINSAHKLIKRSLLKNPDPFA--QIIYNNHSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTA----DFIDRGERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL 772
            V++ I  A  L ++ L + PDPFA  Q+      + T   + + +P WN  + + L  I D + I +WNQR     K S FLG   + A         G +RL L + +    + VRG + + ++  D  S    L+   I+  +G  RG                 S+ +S E S                     LP  +E + +D G+VY+V++ + +  W  P  P  +
Sbjct:   15 VRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFL-GIGDAITITVWNQRKI--HKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSSGNPLA---IVGPSGDVRG----------------PSEDDSSEDS---------------------LPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVV 204

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0077823)

HSP 1 Score: 337.806 bits (865), Expect = 1.003e-100
Identity = 210/617 (34.04%), Postives = 315/617 (51.05%), Query Frame = 2

Query:  656 DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETI-QTNRRSQ-----SSGQIPSFNNQAIL--DMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCI----IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            D  LP  +E ++   G+ Y+VD+N+ + +W  P                 LPPGW+  +   GRVY++DH +R T +  P   R +          +  Q   A + +Q     +   S+  ++ +  T   +         L    E   QS N    +   NR +Q     + GQ  S  N+  L    E+  +   E   +D N         R    +     +       E   R   S+ + L Q         I  +R  + E+S+  I RL   +LR+RL + F  EEGLDYGGV+REW +LLS  + +  + LFE    N+ +L +++P   VN DHL YF FIGR I MA++HG +I + F   FYK +LNK+  ++D E +D   YNSL+W++DNNID+  L L FS++ EV GQ+   +L ENG    VT+ +K +YI    ++ ++    +Q +   +G  EV+P + L   +E E E++L G+ ++ +EDWQ+NT  +H   N   + WFW+ V     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY SY  L +KLT AIEET GF
Sbjct:  362 DEPLPAGWEIRLDQYGRRYYVDHNTRSTYWEKPTP---------------LPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMH--------FQHWQGQRAHVVSQGN--QRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDNRVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKG-VYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSL-QINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNG---PQRFCIEKV--GKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGF 946

HSP 2 Score: 55.4546 bits (132), Expect = 2.292e-7
Identity = 40/146 (27.40%), Postives = 62/146 (42.47%), Query Frame = 2

Query:  470 PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCL---GLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR 898
            P +  +    H  G    + ++ +Q+ L YS  Q            +DE+   P           LP  +E+KI    +VYFV++ +    W  P      R  G    L   G LPPGW+   + +G  +F+DH +R T + DPR
Sbjct:  426 PNSERLMHFQHWQGQRAHVVSQGNQRYL-YSQQQQQPTAVTAQVTQDDEDALGP-----------LPDGWEKKIQSDNRVYFVNHKNRTTQWEDP------RTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPR 553

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0077824)

HSP 1 Score: 337.806 bits (865), Expect = 1.003e-100
Identity = 210/617 (34.04%), Postives = 315/617 (51.05%), Query Frame = 2

Query:  656 DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETI-QTNRRSQ-----SSGQIPSFNNQAIL--DMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCI----IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            D  LP  +E ++   G+ Y+VD+N+ + +W  P                 LPPGW+  +   GRVY++DH +R T +  P   R +          +  Q   A + +Q     +   S+  ++ +  T   +         L    E   QS N    +   NR +Q     + GQ  S  N+  L    E+  +   E   +D N         R    +     +       E   R   S+ + L Q         I  +R  + E+S+  I RL   +LR+RL + F  EEGLDYGGV+REW +LLS  + +  + LFE    N+ +L +++P   VN DHL YF FIGR I MA++HG +I + F   FYK +LNK+  ++D E +D   YNSL+W++DNNID+  L L FS++ EV GQ+   +L ENG    VT+ +K +YI    ++ ++    +Q +   +G  EV+P + L   +E E E++L G+ ++ +EDWQ+NT  +H   N   + WFW+ V     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY SY  L +KLT AIEET GF
Sbjct:  362 DEPLPAGWEIRLDQYGRRYYVDHNTRSTYWEKPTP---------------LPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMH--------FQHWQGQRAHVVSQGN--QRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDNRVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKG-VYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSL-QINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNG---PQRFCIEKV--GKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGF 946

HSP 2 Score: 55.4546 bits (132), Expect = 2.292e-7
Identity = 40/146 (27.40%), Postives = 62/146 (42.47%), Query Frame = 2

Query:  470 PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCL---GLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR 898
            P +  +    H  G    + ++ +Q+ L YS  Q            +DE+   P           LP  +E+KI    +VYFV++ +    W  P      R  G    L   G LPPGW+   + +G  +F+DH +R T + DPR
Sbjct:  426 PNSERLMHFQHWQGQRAHVVSQGNQRYL-YSQQQQQPTAVTAQVTQDDEDALGP-----------LPDGWEKKIQSDNRVYFVNHKNRTTQWEDP------RTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPR 553

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Su(dx) (gene:FBgn0003557 transcript:FBtr0339529)

HSP 1 Score: 337.806 bits (865), Expect = 1.003e-100
Identity = 210/617 (34.04%), Postives = 315/617 (51.05%), Query Frame = 2

Query:  656 DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETI-QTNRRSQ-----SSGQIPSFNNQAIL--DMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCI----IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            D  LP  +E ++   G+ Y+VD+N+ + +W  P                 LPPGW+  +   GRVY++DH +R T +  P   R +          +  Q   A + +Q     +   S+  ++ +  T   +         L    E   QS N    +   NR +Q     + GQ  S  N+  L    E+  +   E   +D N         R    +     +       E   R   S+ + L Q         I  +R  + E+S+  I RL   +LR+RL + F  EEGLDYGGV+REW +LLS  + +  + LFE    N+ +L +++P   VN DHL YF FIGR I MA++HG +I + F   FYK +LNK+  ++D E +D   YNSL+W++DNNID+  L L FS++ EV GQ+   +L ENG    VT+ +K +YI    ++ ++    +Q +   +G  EV+P + L   +E E E++L G+ ++ +EDWQ+NT  +H   N   + WFW+ V     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY SY  L +KLT AIEET GF
Sbjct:  362 DEPLPAGWEIRLDQYGRRYYVDHNTRSTYWEKPTP---------------LPPGWEIRKDGRGRVYYVDHNTRKTTWQRPNSERLMH--------FQHWQGQRAHVVSQGN--QRYLYSQQQQQPTAVTAQVTQDDEDALGPLPDGWEKKIQSDNRVYFVNHKNRTTQWEDPRTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPRPGAPKGAKG-VYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSL-QINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVKDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRHYNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMGSNG---PQRFCIEKV--GKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGF 946

HSP 2 Score: 55.4546 bits (132), Expect = 2.292e-7
Identity = 40/146 (27.40%), Postives = 62/146 (42.47%), Query Frame = 2

Query:  470 PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCL---GLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR 898
            P +  +    H  G    + ++ +Q+ L YS  Q            +DE+   P           LP  +E+KI    +VYFV++ +    W  P      R  G    L   G LPPGW+   + +G  +F+DH +R T + DPR
Sbjct:  426 PNSERLMHFQHWQGQRAHVVSQGNQRYL-YSQQQQQPTAVTAQVTQDDEDALGP-----------LPDGWEKKIQSDNRVYFVNHKNRTTQWEDP------RTQGQEVSLINEGPLPPGWEIRYTAAGERFFVDHNTRRTTFEDPR 553

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:563633])

HSP 1 Score: 426.017 bits (1094), Expect = 1.270e-135
Identity = 278/829 (33.53%), Postives = 423/829 (51.03%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVP--------KCLRKIGDFSCLGLLPPGWDSLQSDSGRVY--------FIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRIS---------RPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+    T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     S  VRG + V +   D                   S G   + S+                   F ND                 LP  +E + +  G++ ++++ + +  W  P  P        + L  + D +   + P G   + +D  RV           +++SRT  ++ P L      P+GY     + Q     +  Q+G  T     R  R  SN    E          L    EI N +T     +  N R+    Q       A L + L+ S       ++  +   P  L+ +  S          P    +   K ++  KL+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ DN+I    L+ TF +E   +G++   +L  NG+ IPVT ++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW  V SY E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A+  +LPKAHTCFNR+D+PPY SY  L+DKL  AIEETCGFAV
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGK-SDSITISVWNHKKIHKKQGAGFLGCVRLLSNSINRLKDTGYQRLDLNKLGPNDSDTVRGQIVVSLQSRD----------------RIGSGGPVVDCSRL------------------FDND-----------------LPDGWEERRTASGRIQYLNHITRSTQWERPTRPASEYSSPGRPLSCLVDENTPIMTPNGAAGVPADDPRVQERRVRSQRHRNYMSRTHLHTPPDL------PEGYEQRTTQ-QGQVYFLHTQTGVST-WHDPRVPRDLSNVNCEE-------LGPLPPGWEIRNTATGRVYFVDHNNRTT---QFTDPRLSANLHLVLNPSPNGSRAAVEAQSSSRPGQLKEQAQSVVSPGNLPEDPECLTVPKYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETCGFAV 764

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2744])

HSP 1 Score: 383.645 bits (984), Expect = 5.149e-120
Identity = 187/404 (46.29%), Postives = 271/404 (67.08%), Query Frame = 2

Query: 1298 PTSNYILMNKNNIEIKLR---DLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            P    + M + + E+ +R   DL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN    L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+    +L  NG+ IPVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   +  E E+++ G+ +I + DW+ NTRLKHC+ +  ++KWFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+GS GS  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  330 PVQMEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSTGSAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730

HSP 2 Score: 128.642 bits (322), Expect = 5.791e-30
Identity = 88/314 (28.03%), Postives = 144/314 (45.86%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSI-----------------------PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   S  VRG + V +            +D   +                       P   + P      G+ R L + N +Q L+    ++       H+  N     RP      H    LP  +E++ +  G+VYF+   +G   W+ P +P+ L  +   SC  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKT-DSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLVENEGPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQN-----RPH----GHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSV---SCEELGPLPPGWEIRSTVSGRIYFVDHNNRTTQFTDPRL 312

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: itcha (itchy E3 ubiquitin protein ligase a [Source:ZFIN;Acc:ZDB-GENE-120919-4])

HSP 1 Score: 352.829 bits (904), Expect = 4.450e-107
Identity = 219/627 (34.93%), Postives = 321/627 (51.20%), Query Frame = 2

Query:  629 PGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTA-VTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAIL---------DMELSMSNISE--DKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            P    I++G L  P  +E++    G+VY+VD+      W  P                 LPPGW+      GRVY++DHI+RTT +  P     LE+   Y     + QN  + ++            R ++R   G + + + TA   F+ L        + T+    +        S Q      Q +L         +M  ++  I    D N      + P   +    + P   Y+     + + K+   R   ++L   +   I  SR  + E+SF+ I    P DLR+RL + F  EEGLDYGGVAREW +LLS  + +  + LFE  G  D   L+++P   +N DHL YF FIGR I MA+FHG +ID  F   FYK ILNK   L+D E +D   YNSL+W++DNNI++  L + FS+++E+ G++   +L  +G  I VT+ +K +YI    ++ LS    EQ +   +G  +VLPQQ L   +  E EV+L G+ EI + DWQ+N+  +H   +   I WFW+ V     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +   N   LP++HTCFNRLDLPPY SY+ L +KL  AIEET GF 
Sbjct:  266 PRAPAITNGPL--PPGWEQRADQNGRVYYVDHIEKRTTWERPEP---------------LPPGWERRLDPMGRVYYVDHINRTTTWQRP----TLESVRNY----EEWQNQRSQLQGAM--------QRFNQRFIYGLQEQLAPTANKEFDPLGPLPSGWEKRTDSNRRMYFVHHPTRSTQWEDPRTQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSSLENGPQITYV----RDFKAKVHYFRFWCQQLSMPQHIKIHVSRKTLFEDSFQQIMGCHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAG-KDNYCLQINPASSINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLALKDLESIDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEENKEEYIRLVAEWRLSRGVEEQTQAFLEGFNDVLPQQYLQYFDAKELEVMLCGMQEIDLGDWQRNSIYRHYARSSKQIVWFWQFVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNG---PQKFCIEKVGKEN--WLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFG 849

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-131120-84])

HSP 1 Score: 330.872 bits (847), Expect = 2.098e-99
Identity = 202/590 (34.24%), Postives = 300/590 (50.85%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSF------NNQAILDMELSMSNISEDKNIDCNNELAPLP-------------------LQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLC---------IIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            LPPGW+  +   GR Y++DH +RTT +  P+ L     P G+    R++ N G +      Y     R+ + +R +  +     Q     + L+ +    NQ   ++ ++ +          P +      N++       + +   ED          PLP                    +    S P +    + K       R  R KL     + LC          I  SR  + E+SF+ I   KP DLR+RL + F  EEGLDYGG+AREW +LLS  + +  + LFE  G ++   L+++P   +N DHL YF FIGR I MA+FHG +ID  F   FYK +L+K+  L+D E +D   YNSL+W+RDNNI++  L + FS++ E+ G++   DL E+G  + VT+ +K +YI    ++  S     Q +    G  EV+P Q L   +E E EV+L G+ E+ ++DWQ+NT  +H   N   I WFW++V     + R +L+QFVTG+ R+PL GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY S++ L +KL  AIEET GF 
Sbjct:  235 LPPGWEQRKDPHGRTYYVDHNTRTTTWERPQPL-----PLGWE---RRVDNRGRIY-----YVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYSASMMSAENDPLGPLPPGWERRVDTNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSAVTKGPQIAYERSFRWKLAHF--RYLCQSNALASHVKITVSRQTLFEDSFQQIMNFKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSN-YCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKISSHDLKEDGANVQVTEENKEEYIGLMAEWRFSRGVESQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNG---PQKFCIEKV--GKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFG 803

HSP 2 Score: 55.4546 bits (132), Expect = 3.110e-7
Identity = 41/130 (31.54%), Postives = 61/130 (46.92%), Query Frame = 2

Query:  512 SSRGLTAENSQQS-LRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR 898
            S R      SQ+S L+ + +Q +QR     S  + EN+   GP         LP  +ER++    +VYFV++N+    W  P       +         LP GW+   +  G  YF+DH +RTT +SDPR
Sbjct:  300 SVRNFEQWQSQRSQLQGAMHQFNQRYLYSASMMSAENDPL-GP---------LPPGWERRVDTNDRVYFVNHNTKTTQWEDP-------RTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPR 412

HSP 3 Score: 51.9878 bits (123), Expect = 3.557e-6
Identity = 26/95 (27.37%), Postives = 44/95 (46.32%), Query Frame = 2

Query:  617 NNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            +  +P   Q + G   LP  +E++    G+ Y+VD+N+    W  P                 LP GW+    + GR+Y++DH +RTT +  P +
Sbjct:  219 DGGKPAQQQPTGGVEPLPPGWEQRKDPHGRTYYVDHNTRTTTWERPQP---------------LPLGWERRVDNRGRIYYVDHNTRTTTWQRPTM 298

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: wwp1 (WW domain containing E3 ubiquitin protein ligase 1 [Source:ZFIN;Acc:ZDB-GENE-131120-84])

HSP 1 Score: 317.775 bits (813), Expect = 7.227e-97
Identity = 161/366 (43.99%), Postives = 227/366 (62.02%), Query Frame = 2

Query: 1376 LMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            L Q  +  I  SR  + E+SF+ I   KP DLR+RL + F  EEGLDYGG+AREW +LLS  + +  + LFE  G ++   L+++P   +N DHL YF FIGR I MA+FHG +ID  F   FYK +L+K+  L+D E +D   YNSL+W+RDNNI++  L + FS++ E+ G++   DL E+G  + VT+ +K +YI    ++  S     Q +    G  EV+P Q L   +E E EV+L G+ E+ ++DWQ+NT  +H   N   I WFW++V     + R +L+QFVTG+ R+PL GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY S++ L +KL  AIEET GF 
Sbjct:  222 LAQHFILCITVSRQTLFEDSFQQIMNFKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSN-YCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMFFSVDMEILGKISSHDLKEDGANVQVTEENKEEYIGLMAEWRFSRGVESQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNG---PQKFCIEKV--GKDTWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFG 581

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500])

HSP 1 Score: 374.015 bits (959), Expect = 7.918e-120
Identity = 182/398 (45.73%), Postives = 272/398 (68.34%), Query Frame = 2

Query: 1286 RISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + + P  ++I+  + ++  KL+ LR +L  L  Q   C +E SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+L   +L  NG+ + VT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L    + E E+++ G+ +I I DW+ NTRLKHCL N  +++WFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+G+ G   P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EET GFAV
Sbjct:   52 QFTDPRLHHIIKYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAG---PRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAV 443

HSP 2 Score: 63.1586 bits (152), Expect = 1.083e-9
Identity = 30/87 (34.48%), Postives = 54/87 (62.07%), Query Frame = 2

Query:  767 CLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSD 1021
            C R +   +C  LG LP GW+   + SGR+YF+DH +RTTQ++DPRL   ++        ++ L++  ++++ Q+G + ++E SR +
Sbjct:   12 CFRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIIKYERDLVQKLKVLRHELSLLQPQAG-HCRVEVSREE 97

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500])

HSP 1 Score: 380.948 bits (977), Expect = 5.607e-119
Identity = 184/394 (46.70%), Postives = 269/394 (68.27%), Query Frame = 2

Query: 1298 PTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            PT  Y    + ++  KL+ LR +L  L  Q   C +E SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+L   +L  NG+ + VT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L    + E E+++ G+ +I I DW+ NTRLKHCL N  +++WFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EET GFAV
Sbjct:  329 PTQRY----ERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAV 715

HSP 2 Score: 106.301 bits (264), Expect = 6.616e-23
Identity = 81/292 (27.74%), Postives = 130/292 (44.52%), Query Frame = 2

Query:  149 PDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSIPTNLSMPQIIHINGSSRGLT-----------------------AENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHG--DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            PDPFA+I+ +    C  T+  + T +P WN+ + + +  + D + I IWN +   +K+ + FLG   L ++ I R    G +RL L + N   S  VRG + V +            +D   +  N     +    G  R L+                        E+  Q  R    +      +EH  +         P   SHG     LP  +E++ +  G+VYF+   +G   W+ P +P+ L  + +   LG LP GW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   19 PDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKM-DSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGA--LYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQT---------PLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSV-NCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRL 297

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500])

HSP 1 Score: 380.178 bits (975), Expect = 1.780e-118
Identity = 184/394 (46.70%), Postives = 269/394 (68.27%), Query Frame = 2

Query: 1298 PTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            PT  Y    + ++  KL+ LR +L  L  Q   C +E SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+L   +L  NG+ + VT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L    + E E+++ G+ +I I DW+ NTRLKHCL N  +++WFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EET GFAV
Sbjct:  344 PTQRY----ERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAV 730

HSP 2 Score: 119.013 bits (297), Expect = 9.135e-27
Identity = 88/312 (28.21%), Postives = 145/312 (46.47%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDS-HSIPTNLSMPQIIHINGS-------SRGLT-----------------------AENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHG--DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI V+L +  A  L KR   + PDPFA+I+ +    C  T+  + T +P WN+ + + +  + D + I IWN +   +K+ + FLG   L ++ I R    G +RL L + N   S  VRG + V +   D   ++ + +    ++  +G+        R L+                        E+  Q  R    +      +EH  +         P   SHG     LP  +E++ +  G+VYF+   +G   W+ P +P+ L  + +   LG LP GW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIRVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKM-DSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQT---------PLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSV-NCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRL 312

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500])

HSP 1 Score: 373.244 bits (957), Expect = 6.180e-116
Identity = 183/394 (46.45%), Postives = 268/394 (68.02%), Query Frame = 2

Query: 1298 PTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            PT  Y    + ++  KL+ LR +L  L  Q   C +E SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+L   +L  NG+ + VT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L    + E E+++ G+ +I I DW+ NTRLKHCL N  +++WFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+G+ G   P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EET GFAV
Sbjct:  344 PTQRY----ERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAG---PRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAV 727

HSP 2 Score: 106.686 bits (265), Expect = 6.293e-23
Identity = 79/303 (26.07%), Postives = 135/303 (44.55%), Query Frame = 2

Query:  122 LIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDS-HSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQ--------------------------------SSQRNSKEHSFSNDENNSRPGPSQISHG--DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            L+    +  PDPFA+I+ +    C  T+  + T +P WN+ + + +  + D + I IWN +   +K+ + FLG   L ++ I R    G +RL L + N   S  VRG + V +   D   ++ + +    ++  +G+ R +      ++   +G                                    QR   +     +       P   SHG     LP  +E++ +  G+VYF+   +G   W+ P +P   R +   +C  LG LP GW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   14 LLVTCFIGLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKM-DSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGTLRHMCQTGGPRATSDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP---RDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRL 312

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: crybb2 (crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-5866500])

HSP 1 Score: 373.244 bits (957), Expect = 1.268e-115
Identity = 183/394 (46.45%), Postives = 268/394 (68.02%), Query Frame = 2

Query: 1298 PTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            PT  Y    + ++  KL+ LR +L  L  Q   C +E SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+L   +L  NG+ + VT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L    + E E+++ G+ +I I DW+ NTRLKHCL N  +++WFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+G+ G   P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EET GFAV
Sbjct:  364 PTQRY----ERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAG---PRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAV 747

HSP 2 Score: 105.916 bits (263), Expect = 1.173e-22
Identity = 84/313 (26.84%), Postives = 135/313 (43.13%), Query Frame = 2

Query:  122 LIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSIPTNLSMPQIIHIN----------GSSRGLT-----------------------AENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHG--DLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            L+    +  PDPFA+I+ +    C  T+  + T +P WN+ + + +  + D + I IWN +   +K+ + FLG   L ++ I R    G +RL L + N   S  VRG + V +            +D   +      P +  I+          G  R L+                        E+  Q  R    +      +EH  +         P   SHG     LP  +E++ +  G+VYF+   +G   W+ P +P   R +   +C  LG LP GW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   33 LLVTCFIGLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKM-DSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLMFPCLYPVMYCISYIFRALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQT---------PLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP---RDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRL 332

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:1913563])

HSP 1 Score: 385.185 bits (988), Expect = 1.830e-120
Identity = 227/620 (36.61%), Postives = 332/620 (53.55%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQ-----------NSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L             +GA   + S       R RS R R+    +  +      +L E   +                R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPVT+ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  146 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRN---YMSRTHLHTPPDLPEGYEQ----------------RTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 734

HSP 2 Score: 101.679 bits (252), Expect = 1.651e-21
Identity = 85/312 (27.24%), Postives = 137/312 (43.91%), Query Frame = 2

Query:  125 IKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            +K  L   PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTGLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 317

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:1913563])

HSP 1 Score: 385.185 bits (988), Expect = 3.020e-120
Identity = 227/620 (36.61%), Postives = 332/620 (53.55%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQ-----------NSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L             +GA   + S       R RS R R+    +  +      +L E   +                R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPVT+ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRN---YMSRTHLHTPPDLPEGYEQ----------------RTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747

HSP 2 Score: 110.153 bits (274), Expect = 3.706e-24
Identity = 87/325 (26.77%), Postives = 145/325 (44.62%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:1913563])

HSP 1 Score: 385.185 bits (988), Expect = 3.020e-120
Identity = 227/620 (36.61%), Postives = 332/620 (53.55%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQ-----------NSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L             +GA   + S       R RS R R+    +  +      +L E   +                R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPVT+ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRN---YMSRTHLHTPPDLPEGYEQ----------------RTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747

HSP 2 Score: 110.153 bits (274), Expect = 3.706e-24
Identity = 87/325 (26.77%), Postives = 145/325 (44.62%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:1923038])

HSP 1 Score: 382.874 bits (982), Expect = 1.076e-119
Identity = 181/384 (47.14%), Postives = 265/384 (69.01%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  350 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730

HSP 2 Score: 120.553 bits (301), Expect = 2.095e-27
Identity = 89/312 (28.53%), Postives = 143/312 (45.83%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSIPTNLSMPQIIHINGSSRGLTA--------------ENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQI------SHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +            +D   +  N     +   +G  R L+                    + R+  + S  +        N E     GP Q        H    LP  +E++ +  G+VYF+   +G   W+ P +P   R +   +C  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGT--VYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVR---GPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP---RDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL 313

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:1923038])

HSP 1 Score: 382.874 bits (982), Expect = 2.089e-119
Identity = 181/384 (47.14%), Postives = 265/384 (69.01%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   L +++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  376 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTL-QINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 756

HSP 2 Score: 113.235 bits (282), Expect = 3.771e-25
Identity = 90/335 (26.87%), Postives = 145/335 (43.28%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSIPTNLSMPQIIHINGSSRGLTAENSQQS--------------LRYSGNQSSQRNSKEHSFSNDENNSRPGPSQI------SHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL-----------------------RKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +            +D   +  N     +   +G  R L+    + +               R+  + S  +        N E     GP Q        H    LP  +E++ +  G+VYF+   +G   W+ P +P  L                       R +   +C  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGT--VYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVR---GPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL 339

[Close]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|A9JRZ0|SMUF2_DANRE (E3 ubiquitin-protein ligase SMURF2 OS=Danio rerio OX=7955 GN=smurf2 PE=2 SV=1)

HSP 1 Score: 426.017 bits (1094), Expect = 9.584e-135
Identity = 278/829 (33.53%), Postives = 423/829 (51.03%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVP--------KCLRKIGDFSCLGLLPPGWDSLQSDSGRVY--------FIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRIS---------RPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+    T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     S  VRG + V +   D                   S G   + S+                   F ND                 LP  +E + +  G++ ++++ + +  W  P  P        + L  + D +   + P G   + +D  RV           +++SRT  ++ P L      P+GY     + Q     +  Q+G  T     R  R  SN    E          L    EI N +T     +  N R+    Q       A L + L+ S       ++  +   P  L+ +  S          P    +   K ++  KL+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ DN+I    L+ TF +E   +G++   +L  NG+ IPVT ++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW  V SY E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A+  +LPKAHTCFNR+D+PPY SY  L+DKL  AIEETCGFAV
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGK-SDSITISVWNHKKIHKKQGAGFLGCVRLLSNSINRLKDTGYQRLDLNKLGPNDSDTVRGQIVVSLQSRD----------------RIGSGGPVVDCSRL------------------FDND-----------------LPDGWEERRTASGRIQYLNHITRSTQWERPTRPASEYSSPGRPLSCLVDENTPIMTPNGAAGVPADDPRVQERRVRSQRHRNYMSRTHLHTPPDL------PEGYEQRTTQ-QGQVYFLHTQTGVST-WHDPRVPRDLSNVNCEE-------LGPLPPGWEIRNTATGRVYFVDHNNRTT---QFTDPRLSANLHLVLNPSPNGSRAAVEAQSSSRPGQLKEQAQSVVSPGNLPEDPECLTVPKYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETCGFAV 764

[Close]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9HCE7|SMUF1_HUMAN (E3 ubiquitin-protein ligase SMURF1 OS=Homo sapiens OX=9606 GN=SMURF1 PE=1 SV=2)

HSP 1 Score: 424.476 bits (1090), Expect = 2.484e-134
Identity = 276/820 (33.66%), Postives = 424/820 (51.71%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGS---SRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSG-----AIHWYHPAVPKCL-----RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLR-ALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLK-ELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            SI ++L +  A  L K+   + PDPFA+I+ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + N   +  VRG + V +   D             I   GS    RGL  EN       SG         E           P P   S G            + GG   FV++ S      A    +P V   L     R  G  S    LP G++   +  G+VYF+   +  + + DPR+   +   P G A  +               Y   ++   S+ R  N    +          L    E+ +  +     +  N R+    Q        I++ +  +   S+   +     L    L  +R  R           ++  KL+ LR +L  +  Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN   +L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG++   +L  NGR +PVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   ++ E E+++ G+ +I + DW+ NTRLKHC+ +  +++WFW+ V ++ E++R +LLQFVTGS+R+PL GF+ L+GS G+  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQ--HYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDR------------IGTGGSVVDCRGLL-ENEGTVYEDSGPGRPLSCFME----------EPAPYTDSTG----------AAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQS--PELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFL---------------YEFLLQGHTSEPRDLNSVNCDE------LGPLPPGWEVRSTVSGRIYFVDHNNRTT---QFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYER-----------DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 756

[Close]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q2TAS2|SMUF2_XENLA (E3 ubiquitin-protein ligase SMURF2 OS=Xenopus laevis OX=8355 GN=smurf2 PE=2 SV=1)

HSP 1 Score: 390.963 bits (1003), Expect = 1.268e-121
Identity = 232/618 (37.54%), Postives = 348/618 (56.31%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKL-ESSQTAVTFNLLESSAEINNQSTNHTETIQT-----NRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLP-------LQRRRI--------------SRPTSN-YILMNKNNIEIK-------------------------LRDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L  S  V +N     T I  +       NG    ++S   ++   + S    N  S  H  T         +R+   GQ+   + Q  +          +  N++C  EL PLP           R+               R ++N ++++N+ N ++K                          RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G+L   +L  +G+ IPVT+++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V S+ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A+  +LPKAHTCFNR+D+PPY +Y+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPL--SCFVDEN-----TPITGT-------NGASCGQTSDPRISERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLGNVNCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQQLKEQQPPQVVSLCQLPDEVECLTVPRYKRDLVHKLKSLRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHDMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGELIQHELKPSGKSIPVTEDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIICGLGKIDVSDWKSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DASTNNLPKAHTCFNRIDIPPYETYEKLYEKLLTAIEETCGFAV 750

HSP 2 Score: 114.39 bits (285), Expect = 1.441e-24
Identity = 88/328 (26.83%), Postives = 146/328 (44.51%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI---------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLL----------------------------LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PD FA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D        S  Q++           +G     TA    Q L    R +  +   R + E+S          DEN    G +  S G                               LP  +E++ +  G+VYF+   +G   W+ P VP   R +G+ +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDSFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSITISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIG-----SGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGASCGQTSDPRISERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLGNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|A2A5Z6|SMUF2_MOUSE (E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus OX=10090 GN=Smurf2 PE=1 SV=1)

HSP 1 Score: 385.185 bits (988), Expect = 2.113e-119
Identity = 227/620 (36.61%), Postives = 332/620 (53.55%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQ-----------NSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L             +GA   + S       R RS R R+    +  +      +L E   +                R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPVT+ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRN---YMSRTHLHTPPDLPEGYEQ----------------RTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747

HSP 2 Score: 110.153 bits (274), Expect = 2.592e-23
Identity = 87/325 (26.77%), Postives = 145/325 (44.62%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9HAU4|SMUF2_HUMAN (E3 ubiquitin-protein ligase SMURF2 OS=Homo sapiens OX=9606 GN=SMURF2 PE=1 SV=1)

HSP 1 Score: 383.645 bits (984), Expect = 6.157e-119
Identity = 229/614 (37.30%), Postives = 334/614 (54.40%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQT-----NRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL--------------------------------------------RDLRSKLKELMQK--------KLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+      R       Y++  R L  S  V +N     T I  +      +  T  +SS   +    + S    N  S  H  T         +R+   GQ+   + Q  +          +  NI+C  EL PLP      +  T     ++ NN   +                                             RDL  KLK L Q+          C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ +N+I    L+ TF +E   +G++   +L  NG+ IPV + +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I + DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A   +LPKAHTCFNR+D+PPY SY+ L++KL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWE-----RPTRPASEYSSPGRPL--SCFVDEN-----TPISGT------NGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCE-ELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747

HSP 2 Score: 110.538 bits (275), Expect = 1.849e-23
Identity = 87/325 (26.77%), Postives = 145/325 (44.62%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENNSRPGPSQISHGDLLLPINFERKI----------------------------SDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            + ++L +  A  L+K+   + PDPFA+++ +    C  T+  + T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN    G +  + G    P   ER++                            +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGK-SDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD--RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330

[Close]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A087TNR4 (E3 ubiquitin-protein ligase SMURF2 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_05256 PE=4 SV=1)

HSP 1 Score: 453.366 bits (1165), Expect = 5.407e-145
Identity = 253/611 (41.41%), Postives = 369/611 (60.39%), Query Frame = 2

Query:  650 HGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQ-IPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            H  + LP  +E + +  G+VYF   ++G   W+ P VP+ L  I +   LG LP GW+   + +GRVY++DH +RTTQ++DPRL  +L         ++ + N+                    RR S+   +E +   +   + +SS  ++  S NHT +  T   S      +P   N A+  +E+S +               P   QRR + +               KL  LR +L  L  Q   C +E SR++I EES+R I +++P DLRKRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+   D D   L+++    VN +HL YFHF+GRVIG+A+FHG+YID  F   FYK +LNK   L+D E +D  +  SL WM +NNI    ++ +FS+E + FGQ+++ +L  NGR IPVTD +K +Y+  YV+Y       +Q+  ++KG  E++PQ +L + +E E E+++ G+ +I +EDW+ NTRLKHC  +  ++KWFW+ V SY+E+QR +LLQFVTGSSR+PL GF+ L+GS G+  P+LFTI +I +A+  +LPKAHTCFNR+D+PPY SY+ L++KLT AIEETCGFAV
Sbjct:   52 HQAVELPDGYEIRTTQQGQVYFYHVSTGVSTWHDPRVPRDLAGI-NVGDLGPLPNGWEMRHTANGRVYYVDHNNRTTQFTDPRLSSSL--------AVQNMLNT--------------------RRNSS---IEDNTNILNGRVPDSSQAVS--SVNHTPSAITPSNSAPPLHPLPPTINPAV-PLEISDT---------------PYSKQRRDLVQ---------------KLAALRQELHALQPQSGHCRLEVSREDIFEESYRLIMKMRPKDLRKRLMVKFRSEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYTRD-DIYTLQINHDSSVNPEHLSYFHFVGRVIGLAVFHGHYIDGGFTLPFYKMLLNKPITLDDIEAVDPELQRSLKWMLENNITSV-IDTSFSVEHDAFGQIQVHELKPNGREIPVTDENKREYVRLYVNYRFMRGIEQQFLALQKGFNELIPQHLLKSFDEKELELVIGGLGKIDLEDWKMNTRLKHCNPDTNIVKWFWKAVDSYSEEQRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHLI-DASTDNLPKAHTCFNRIDIPPYESYEKLYEKLTQAIEETCGFAV 594

[Close]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A088A7D3 (E3 ubiquitin-protein ligase OS=Apis mellifera OX=7460 GN=lack PE=4 SV=1)

HSP 1 Score: 455.677 bits (1171), Expect = 4.604e-142
Identity = 245/608 (40.30%), Postives = 371/608 (61.02%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLES-SQTAVTFNLLESSAEINNQSTNHTETIQ-TNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLP-PYPSYKCLHDKLTCAIEETCGFAV 2476
            LP  +E + +  G+VYF    +G+  W+ P +P+ L      + LG LP GW+  Q+ SGRVYF+DH +RTTQ++DPRL   +      + ++ + QNS     + S     I  S         T++E+ +  ++  N+++  +   N S N++ T++ T + +Q+  ++P    + ++D EL                   LP  +R               ++  KL+ LR++L  L  Q   C +E SR+ I EES+R I +++P D+RKRL++KF  EEGLDYGGVAREWLYLLS  + + ++GLF+ + D D   L+++P   +N +HL YFHF GR+IG+A+FHG++ID  F   FYK +LNK   L D E +D  ++ SL WM +N+ID   L+ TFS+E   FG L+  +L   G+ IPVT+ +K +Y+  YV+Y       +Q+  ++KG +E++P Q+L   +E E E+++ G+  I I DW+ +TRLKHC  + P++KWFW++V SY E+ R +LLQFVTGSSR+PL GF+ L+GS G+  P+LFTI  + +A   +LPKAHTCFNR+D+P  YPSY+ + DKLT A+EETCGFAV
Sbjct:  352 LPRGYEMRKTQQGQVYFYHVPTGSSTWHDPRIPRDLPANELANELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQI-----ISNLLNRRQNSTNQTTSNSNNSATINES---------TEIETPASPSIQCNIVQQRSRTENGSNNNSPTLENTAQNAQTVSELP----KELMDNEL-------------------LPKYKR---------------DLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRD-DNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGV-LDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAV-DAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAV 904

[Close]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A0M9A0C8 (E3 ubiquitin-protein ligase SMURF2 (Fragment) OS=Melipona quadrifasciata OX=166423 GN=WN51_01160 PE=4 SV=1)

HSP 1 Score: 452.981 bits (1164), Expect = 4.433e-141
Identity = 244/614 (39.74%), Postives = 371/614 (60.42%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQ------TAVTFNLLESSAEINNQSTNHTETIQ--TNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLP-PYPSYKCLHDKLTCAIEETCGFAV 2476
            LP  +E + +  G+VYF    +G+  W+ P +P+ L      + LG LP GW+  Q+ SGRVYF+DH +RTTQ++DPRL   +      + ++ + QNS             I ++ S+   S     ES++      +++  N+++      N S N++ T++  + + +Q+  ++P    + ++D EL                   LP  +R               ++  KL+ LR++L  L  Q   C +E SR+ I EES+R I +++P D+RKRL++KF  EEGLDYGGVAREWLYLLS  + + ++GLF+ + D D   L+++P   +N +HL YFHF GR+IG+A+FHG++ID  F   FYK +LNK   L D E +D  ++ SL WM +N+ID   L+ TFS+E   FG L+  +L   G+ IPVT+ +K +Y+  YV+Y       +Q+  ++KG +E++P Q+L   +E E E+++ G+  I I DW+ +TRLKHC  + P++KWFW++V SY E+ R +LLQFVTGSSR+PL GF+ L+GS G+  P+LFTI  + +A   +LPKAHTCFNR+D+P  YPSY+ + DKLT A+EETCGFAV
Sbjct:  337 LPRGYEMRKTQQGQVYFYHVPTGSSTWHDPRIPRDLPANELANELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQI-----ISNLLNRRQNS------------TINQTTSNSNNSTAAANESAEIETPVSSSIQSNIVQQRQRTENGSNNNSPTLENTSTQNAQTVSELP----KELMDNEL-------------------LPKYKR---------------DLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRD-DNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGV-LDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAV-DAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAV 892

[Close]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: W8BRF4 (E3 ubiquitin-protein ligase Smurf1 OS=Ceratitis capitata OX=7213 GN=SMUF1 PE=2 SV=1)

HSP 1 Score: 441.425 bits (1134), Expect = 6.647e-141
Identity = 244/608 (40.13%), Postives = 358/608 (58.88%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCL-RKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRK--LQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP  +E + +  G+VYF    +G   W+ P +P+    +      +G LP GW+  ++ SGRVYF+DH +RTTQ++DPR    +        +IR+  L N+G    N SG    +  +       NG+ ++    A +  L   S+ + N +   + T Q  R S +S   P+   Q +L+                  EL  LP  RR               ++  K+R LR++L+ L  Q   C +E SR+ I EES+R I +++P D+RKRL++KF  EEGLDYGGVAREWL+LLS  + + ++GLF+ + D D   L+++P   VN DHL YFHF+GR++G+A+FHG+ +D  F   FYK +LNK   L D E +D  ++ SL WM +NNI    ++ TFS+E   FG L + +L  +G  I VT+++K +Y++ YV+Y       +Q+  ++KG  E++P Q+L   +E E E+++ GIS I + DW+ NTRLKHC      + WFW+VV SY+ + R +LLQFVTGSSR+PL GF  L+GS G+  P+LFTI +  +    +LPKAHTCFNR+DLPPY +Y+ L DKLT A+EETCGFAV
Sbjct:    3 LPAGYEMRTTQQGQVYFFHTPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRFNCNI------LQMIRRGTLSNTG----NSSGTVAAMVST------GNGSIVQQLSPAASHPLSNGSSGVANSTNGGSSTPQ--RVSNNSAAPPADLPQGLLE----------------GAEL--LPKYRR---------------DLVSKMRALRTELQTLQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRD-DHYTLQINPDSGVNPDHLSYFHFVGRILGIAVFHGHCLDGGFTTPFYKQLLNKPITLSDIEGVDPELHRSLTWMLENNITGI-IDSTFSVENNSFGALVVHELKPSGSSILVTEDNKREYVKLYVNYRFMRGIEQQFLALQKGFCELVPSQLLRPFDERELELVIGGISSIDVNDWRINTRLKHCTPETSQVVWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTTDVPTQNLPKAHTCFNRIDLPPYENYQLLCDKLTQAVEETCGFAV 557

[Close]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Match: E9GIQ7 (E3 ubiquitin-protein ligase OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_318414 PE=4 SV=1)

HSP 1 Score: 449.129 bits (1154), Expect = 1.103e-140
Identity = 281/859 (32.71%), Postives = 436/859 (50.76%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSH---SIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWY----------------------------------------HPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIER-----------SRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            +I ++L I  A  L K+ L + PDPFA++       C  T+  + + +P WN+ F + +    D + I +WN +   +K+   FLG   + ++ I R    G +RL L R NA  +  V+G + + ++  + H   S   N + P +               Q  +   GN +S  +      +   + + P           LP  +E + +  G+ Y+V+  S +  W                                         HPAVP+  ++         LP G++  ++  G+VYF    +  + + DPR+ R L          R L   GA+       +T   R             +D R S G  L+        NLL      N   ++H         SQ    +P  N  A  +     + ++       N+  AP P+            +   K ++  K++ LR++L+ +  Q   C +E SR  I EES+R+I +L+P DLRKRL++KF  EEGLDYGGVAREWL+LLS+ + +  +GLF      D   L V+P   VN +HL YFHF+GR++G+A+FHG+++D  F   FYK +LNK   L+D   +D  ++ SL WM +++I    ++ TFS+E   +G +++ DL  +GR + V++ +K +Y+  YV Y       +Q+  ++KG +E++P  +L   +E E E+L+ GI +I   DWQ NTRLKHC    P+++WFW++V SY+E+ R +LLQFVTGSSR+PL GF+ L+GS G+  P+LFTI +I +A   +LPKAHTCFNRLDLPPYPSY+ +H+KLT A+EETCGFAV
Sbjct:   12 AIKIRLTILCAKNLAKKDLFRLPDPFAKVSVEGSGQCHSTDTCKSSLDPKWNQHFDLYI-GKSDAITISVWNHKKIHKKEGGGFLGCVRIMSNAIQRLKDTGYQRLDLTRANANDTDQVKGQIVISLLSREQHNSSSCHENSATPVV---------------QTVVDALGNVASPASPAGIQETATASVTSP-----------LPQGWEERRTSSGRRYYVNPESRSSQWTRPIVESSSGAQASSSGGSSVSASNGRESRRSHSSRERDKRHPAVPQQQQQQSRRPLANDLPDGYEMRKTGQGQVYFYHVTTGISTWYDPRIPRDL----------RDLPMPGALPPGWEMRHTPSGRIYFVDHNNRTTQFTDPRLSTGLALQ--------NLL------NLHRSDHRAA-----PSQPQPTLPPPNAVATSENGACAAPVA-------NSTSAPPPMATPLPDEKQEEALPRYKRDLVAKIKILRTELQTMQPQSGHCRLEVSRTEIFEESYRSIMKLRPKDLRKRLMVKFRGEEGLDYGGVAREWLHLLSQQMLNPYYGLF-CYSREDNYTLHVNPDSSVNPEHLSYFHFVGRIMGLAVFHGHHLDGGFTLPFYKMLLNKAIVLDDITHVDPELHRSLTWMLESDITGV-IDTTFSVEHNAYGAIQVHDLKPDGRDLIVSEENKREYVRLYVSYRFMRGIEQQFLSLQKGFHELIPANLLRPFDERELELLIGGICKIDTADWQANTRLKHCTAETPVVQWFWQIVESYSEELRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHLI-DAPCENLPKAHTCFNRLDLPPYPSYEKMHEKLTQAVEETCGFAV 804

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: SMURF2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103046280])

HSP 1 Score: 390.963 bits (1003), Expect = 1.974e-122
Identity = 228/619 (36.83%), Postives = 340/619 (54.93%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQ-----------NSGAVIKNQSGYYTKIERSRSDRRRS--------------NGTKLESSQTAVTFNL----------------------------LESSAEINNQSTNHTETI-QTNRRSQSSGQIPSFNNQAILD-------MELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ +GR+ +++HI+RTTQ+  P           Y++ +R L             +GA     S    +  R RS R R+               G +  ++Q    + L                            L    EI N +T     +   NR +Q +    S N   +L+         +   NIS    +   + L  +P +   ++ P        K ++  KL+ LR +L +   Q   C IE  R+ I EES+R + +++  DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   LED E +D  ++NSLVW+ +N+I    L+ TF +E   +GQ+   +L  NG+ IPV++ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V ++ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+ FTI  I +A+  +LPKAHTCFNR+D+PPY SY  L+DKL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTATGRIQYLNHITRTTQWERP-----TRPASEYSSPVRPLSCVLDENTPVLTPNGATSVQPSDPRAQERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNPSPNGCRAGVEGQNISRHPQLKDPSPLCNVPEEAECLTVPKY------KRDLVHKLKVLRQELSQAQPQAGHCRIEVCREEIFEESYRQVMKMRQKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLEDMESVDPDLHNSLVWILENDITGV-LDHTFCVEHSAYGQIIQHELKPNGKTIPVSEETKKEYVRLYVNWRFLHGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIHDWRANTRLKHCTADSNVVKWFWKAVETFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRQFTIHQI-DASTNNLPKAHTCFNRVDIPPYESYDKLYDKLLTAIEETCGFAV 760

HSP 2 Score: 61.2326 bits (147), Expect = 4.959e-9
Identity = 33/126 (26.19%), Postives = 65/126 (51.59%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEM 445
            + ++L +  A  L K+   + PDPFA+++ +    C  T+    T +P WN+ + + +  + D + I +WN +   +K+ + FLG   L ++ I+R    G ++L L R     +  +RG + V +
Sbjct:   12 VKLRLTVLCAKNLSKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGKL-DSITISVWNHKKIHKKQGAGFLGCVRLLSNVINRLKDTGYQKLDLNRLGPNDNDTIRGQIVVSL 136

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103027089])

HSP 1 Score: 386.341 bits (991), Expect = 5.127e-121
Identity = 185/384 (48.18%), Postives = 263/384 (68.49%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN    L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+    +L  NGR IPVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   +  E E+++ G+ +I + DW+ NTRLKHC+ +  ++KWFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+GS GS  P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  350 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLSDLETVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGKFLQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSTGSAGPRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730

HSP 2 Score: 134.42 bits (337), Expect = 6.244e-32
Identity = 95/315 (30.16%), Postives = 149/315 (47.30%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSI-----------------------PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            SI ++L +  A  L K+   + PDPFA+++ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + NA  S  VRG + V +            +D   +                       P   S P      GS R L + N +Q L+    ++ +  S  H+  N     RP      H    LP  +E++ +  G+VYF+   +G   W+ P +P+ L  +   SC  LG LPPGW+   + SGR+YF+DH +RTTQ++DPRL
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQ--HYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNASDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLLENEGPVFEDSGPGRPLSCFMEEPLPYSDPTGAAGGGSCRALESPNQEQRLQAQRIRNQESRSHAHTPQN-----RPH----GHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSV---SCEELGPLPPGWEIRSTVSGRIYFVDHNNRTTQFTDPRL 312

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: smurf1 (SMAD specific E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103027089])

HSP 1 Score: 380.563 bits (976), Expect = 1.873e-119
Identity = 184/384 (47.92%), Postives = 262/384 (68.23%), Query Frame = 2

Query: 1349 RDLRSKLKELM--------QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            RDL  KLK L         Q   C IE SR+ I EES+R I +++P DL+KRL++KF  EEGLDYGGVAREWLYLL   + +  +GLF+ + DN    L+++P   +N DHL YFHF+GR++G+A+FHG+YI+  F   FYK +L K  QL D E +D  ++ SLVW+ +N+I    L+ TF +E   FG+    +L  NGR IPVT+ +K +Y+  YV++        Q+  ++KG  E++PQ +L   +  E E+++ G+ +I + DW+ NTRLKHC+ +  ++KWFW+ V S+ E++R +LLQFVTGS+R+PL GF+ L+GS G   P+LFTI +I +AN  +LPKAHTCFNR+D+PPY SY+ L++KL  A+EETCGFAV
Sbjct:  301 RDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNI-YTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLSDLETVDPELHKSLVWILENDITSV-LDHTFCVEHNAFGKFLQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSAG---PRLFTIHLI-DANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 678

HSP 2 Score: 88.1965 bits (217), Expect = 1.985e-17
Identity = 70/268 (26.12%), Postives = 116/268 (43.28%), Query Frame = 2

Query:   83 SITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSI-----------------------PTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPK 766
            SI ++L +  A  L K+   + PDPFA+++ +    C  T+  + T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I R    G +RL L + NA  S  VRG + V +            +D   +                       P   S P      GS R L + N +Q L+    ++ +  S  H+  N     RP      H    LP  +E++ +  G+VYF+   +G   W+ P +P+
Sbjct:   12 SIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQ--HYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNASDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLLENEGPVFEDSGPGRPLSCFMEEPLPYSDPTGAAGGGSCRALESPNQEQRLQAQRIRNQESRSHAHTPQN-----RPH----GHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPR 268

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: SMURF2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103046280])

HSP 1 Score: 377.867 bits (969), Expect = 8.217e-118
Identity = 199/467 (42.61%), Postives = 288/467 (61.67%), Query Frame = 2

Query: 1082 LESSAEINNQSTNHTETI-QTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            L    EI N +T     +   NR +Q +    S N   +L+    + + S   N+    E   +P  +R +                 KL+ LR +L +   Q   C IE  R+ I EES+R + +++  DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   LED E +D  ++NSLVW+ +N+I    L+ TF +E   +GQ+   +L  NG+ IPV++ +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V ++ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+ FTI  I +A+  +LPKAHTCFNR+D+PPY SY  L+DKL  AIEETCGFAV
Sbjct:  288 LPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRHPQLKDPSPLCNVPEEAECLTVPKYKRDLVH---------------KLKVLRQELSQAQPQAGHCRIEVCREEIFEESYRQVMKMRQKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLEDMESVDPDLHNSLVWILENDITGV-LDHTFCVEHSAYGQIIQHELKPNGKTIPVSEETKKEYVRLYVNWRFLHGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIHDWRANTRLKHCTADSNVVKWFWKAVETFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRQFTIHQI-DASTNNLPKAHTCFNRVDIPPYESYDKLYDKLLTAIEETCGFAV 733

HSP 2 Score: 56.6102 bits (135), Expect = 1.129e-7
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 2

Query:  773 RKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:  275 RDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 319

HSP 3 Score: 53.9138 bits (128), Expect = 8.835e-7
Identity = 29/109 (26.61%), Postives = 56/109 (51.38%), Query Frame = 2

Query:  137 LLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEM 445
              + PDPFA+++ +    C  T+    T +P WN+ + + +  + D + I +WN +   +K+ + FLG   L ++ I+R    G ++L L R     +  +RG + V +
Sbjct:    2 FFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGKL-DSITISVWNHKKIHKKQGAGFLGCVRLLSNVINRLKDTGYQKLDLNRLGPNDNDTIRGQIVVSL 109

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: itcha (E3 ubiquitin-protein ligase Itchy-like [Source:NCBI gene;Acc:103032591])

HSP 1 Score: 342.428 bits (877), Expect = 4.180e-104
Identity = 218/621 (35.10%), Postives = 311/621 (50.08%), Query Frame = 2

Query:  800 GLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISE-------------DKNIDCNNEL-----------------------APLP-----------------LQRRR---ISRPTSNYILMNKNNIEIKLRDLRSKL-------KELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            G LPPGW+     +GR+YF+DHI + T +  P  L     P G+    R+L   G V      Y   I R+ + +R +                         +S  + E  Q N+RSQ  G +  FN + I  ++  ++  +              +K  D N  +                        PLP                   RRR   I   T    L N   I   +RD ++K+       ++L   +   I  +R  + E+SF+ I    P DLR+RL + F  EEGLDYGGVAREW +LLS  + +  + LFE  G  D   L+++P   +N DHL YF FIGR I MA+FHG +ID  F   FYK ILNK   L+D E +D   YNSL+W+++NN+ + +L + FS+++E+ G++   +L  +G  I VT+ +K +YI    ++ LS    EQ +   +G  EVLPQQ L   +  E EV+L G+ EI + DWQ+NT  +H   +   I WFW+ V     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +   N   LP++HTCFNRLDLPPY SY+ L +KLT AIEET GF 
Sbjct:  195 GPLPPGWEQRVDQNGRLYFVDHIEKRTTWERPEPL-----PPGWE---RRLDPMGRVY-----YVDHISRTTTWQRPTA------------------------ESVRNYEEWQ-NQRSQLQGAMHQFNQRFIYGLQDQLAPAANKEFDPLGPLPQGWEKRTDSNGRMYFVHHPTRTTQWEDPRTQGLLNEKPLPEGWEMRFTVDGIPYFVDHNRRRTTYIDPRTGKSSLENGPQI-TYVRDFKAKVHYFRFWCQQLSMPQHIKIHVNRKTLFEDSFQQIMSFHPQDLRRRLWIIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAG-KDNYCLQINPASSINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPLALKDLESIDPEFYNSLIWIKENNLQECELEMYFSVDKEILGEVTTHELKPDGGNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEVLPQQYLQYFDAKELEVMLCGMQEIDLSDWQRNTIYRHYARSSKQIIWFWQFVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNG---PQKFCIEKVGKEN--WLPRSHTCFNRLDLPPYKSYEQLKEKLTLAIEETEGFG 770

HSP 2 Score: 56.225 bits (134), Expect = 1.380e-7
Identity = 31/89 (34.83%), Postives = 44/89 (49.44%), Query Frame = 2

Query:  629 PGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDP 895
            P  S I++G L  P  +E+++   G++YFVD+      W  P                 LPPGW+      GRVY++DHISRTT +  P
Sbjct:  187 PRVSPINNGPL--PPGWEQRVDQNGRLYFVDHIEKRTTWERPEP---------------LPPGWERRLDPMGRVYYVDHISRTTTWQRP 258

HSP 3 Score: 55.8398 bits (133), Expect = 1.860e-7
Identity = 36/125 (28.80%), Postives = 53/125 (42.40%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVP------------------------KCLRKIGD---------FSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR---LLRALEAPDGY 931
            LP  +ER++   G+VY+VD+ S    W  P                           + +  + D         F  LG LP GW+     +GR+YF+ H +RTTQ+ DPR   LL     P+G+
Sbjct:  229 LPPGWERRLDPMGRVYYVDHISRTTTWQRPTAESVRNYEEWQNQRSQLQGAMHQFNQRFIYGLQDQLAPAANKEFDPLGPLPQGWEKRTDSNGRMYFVHHPTRTTQWEDPRTQGLLNEKPLPEGW 353

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: smurf2 (SMAD specific E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:563633])

HSP 1 Score: 318.161 bits (814), Expect = 6.927e-96
Identity = 203/642 (31.62%), Postives = 308/642 (47.98%), Query Frame = 2

Query:  794 CLGL----LPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTN-------------RRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKL----------------------------------------------RDLRSKLKELMQKKL--------CIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGR--FIPVTDNSKSQYIER------YVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLP--PYPSYKCLHDKLTCAIEETCGFAV 2476
            C GL    LP GW+  +S  GR  +++H++RT Q+  P    A            +    G V   Q G   +  +    R  + G   E S           S E+  +S  H   +                +R+   GQI   + Q  +          +  +++C  +L PLP      S  T     ++ NN   +                                               RDL  KLK L Q+          C +E SR  I EES+R + +++P DL+KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   +N +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L D E +D  ++ SLVW+    ++++D+N+              +  C  GR  F   T N     + R      YV +        Q+  ++KG  E++PQ +L + +E E E+++ G+ +I + DW+ NTRLKHC     +++WFW  V S+ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI +I +A   +LPKAHTCFNR+D+   PY +   ++D++ C  +   G  +
Sbjct:  151 CRGLFPNDLPEGWEERRSTMGRTQYVNHVTRTAQWERPTRPAA------------ECGGPGCVAPGQEGTPLRAGQGADGRATTAG---EPSAAPAA----PGSPELGTRSQRHRNFLSRTHLHQPPDLPEGYEQRTTQQGQIYFLHTQTGVSTWHDPRVPRDLSHVNCE-DLGPLPAGWETRSTATGRVYFVDHNNRTTQFTDPRLSANLHHLLKNRQTQQGPCPPGLPPGPPPPPADEECPVPRYKRDLVQKLKCLRQELALQQSQAGHCRLEVSRTEIFEESYRQVMKMRPRDLKKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAINPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLADMETVDPELHKSLVWI----LNNSDVNILHE---------HTVHSCWGGRVQFCFRTVNKGVPAMIRLAPRRLYVSWRFLRGIEAQFLALQKGFNELIPQPLLKSFDEKELELIIGGLGKIDVSDWKSNTRLKHCTPESSVVRWFWRAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHLI-DAKTDNLPKAHTCFNRIDITRNPYETNNNIYDRVICCADFYGGLII 754

HSP 2 Score: 113.235 bits (282), Expect = 6.298e-26
Identity = 88/343 (25.66%), Postives = 154/343 (44.90%), Query Frame = 2

Query:   74 MSDSIT-------VKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEM----------IKIDSHSI-PTNL---------SMPQIIHINGSSR-----------------GLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQIS-----HGDLL----------LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            MS+++T       ++L +  A  L K+   + PDPFA+I+      C  T+    T +P WN+ + + + S  D + I +WN++   +K+ + FLG   L ++ I R    G +RL L R     +  VRG + + +            +D   + P +L         +M +  ++N  +R                 G  A   + +   +G  +  R +     S     + PG  ++      H + L          LP  +E++ +  G++YF+   +G   W+ P VP   R +   +C  LG LP GW++  + +GRVYF+DH +RTTQ++DPRL
Sbjct:    1 MSNAVTRRNGATKLRLTVLCAKNLAKKDFFRLPDPFAKIMVEGSGECHSTDTVRNTLDPKWNQHYDLYI-SKSDSITISVWNRKKIHKKQGAGFLGCVRLLSNLISRIKDTGYQRLDLCRLGQSSNDTVRGQIVLSLQSRDRIGTGGAVVDCRGLFPNDLPEGWEERRSTMGRTQYVNHVTRTAQWERPTRPAAECGGPGCVAPGQEGTPLRAGQGADGRATTAGEPSAAP--AAPGSPELGTRSQRHRNFLSRTHLHQPPDLPEGYEQRTTQQGQIYFLHTQTGVSTWHDPRVP---RDLSHVNCEDLGPLPAGWETRSTATGRVYFVDHNNRTTQFTDPRL 337

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: nedd4a (NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;Acc:ZDB-GENE-051005-2])

HSP 1 Score: 304.679 bits (779), Expect = 8.965e-94
Identity = 160/362 (44.20%), Postives = 238/362 (65.75%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRK-RLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLC-IVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFG-QLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLK--HCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            ++  R +ILE+S+R I  +K  DL K RL ++F  E+GLDYGGVAREW +L+S+ +F+  +GLFE +   D   L+++P+  + NE+HL YF FIGRV GMA+FHG  +D  F + FYK +L K   L D E +D   YNSL+W+ +N  D ADL+L F I++E+FG Q  L++L  NG  I VT+ +K +YIE  + +   E+  +Q     +G  E++P  ++   +E+E E+L+ G+ ++ + DW+++T  K  +C  N P+I+WFW+ V     ++R +LLQFVTG+SR+P++GF  L   YGS+ P+LFTI++   +    LP+AHTCFNRLDLPPY S+  L +KL  AIE   GF
Sbjct:  138 MKLHRASILEDSYRRIVAVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSA-TDNYTLQINPISGLCNEEHLSYFTFIGRVAGMAVFHGKLLDGFFIRPFYKMMLQKPITLNDMESVDSEYYNSLMWILEN--DPADLDLRFCIDEEMFGQQTHLVELKPNGSEILVTNENKKEYIELVIQWRFVERVQKQINAFMEGFSELIPMDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNSYC-ANHPVIQWFWKAVLLMDGEKRIRLLQFVTGTSRVPMNGFAEL---YGSNGPQLFTIEMWSTSE--KLPRAHTCFNRLDLPPYESFDDLREKLNIAIENAQGF 490

HSP 2 Score: 60.077 bits (144), Expect = 1.676e-9
Identity = 28/83 (33.73%), Postives = 44/83 (53.01%), Query Frame = 2

Query:  653 GDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            G   LP  +E + +  G+ +F+D+N+    W H ++   L     FS   L  PGW+      GR ++IDH ++ TQ+ DPRL
Sbjct:   19 GFSFLPAGWEMRFAPNGRPFFIDHNNRTTTWSHISLSTMLHP--RFSLFPLPHPGWEERTHSDGRTFYIDHNTKNTQWEDPRL 99

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: wwp2 (WW domain containing E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-000607-82])

HSP 1 Score: 269.626 bits (688), Expect = 5.993e-77
Identity = 137/316 (43.35%), Postives = 195/316 (61.71%), Query Frame = 2

Query: 1526 VAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
             +REW +LLS  + +  + LFE  G N+   L+++P   +N DHL YF FIGR I MA++HG +ID  F   FYK +LNK+  ++D E +D   YNSL+W+RDNNI++  L + FS++ E+ G++   DL  +G  I VT+ +K +YI    ++  S    EQ +    G  EV+P Q L   +E E EV+L G+ EI + DWQ++T  +H   +   I WFW+VV     ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +       LP++HTCFNRLDLPPY SY+ L +KL  AIEET GF 
Sbjct:  621 ASREWFFLLSHEVLNPMYCLFEYAGKNN-YCLQINPASSINPDHLAYFRFIGRFIAMALYHGKFIDTGFSLPFYKRMLNKKLTIKDLESIDPEFYNSLIWLRDNNIEECGLEMFFSVDMELLGKVSSHDLKPDGSDILVTEENKEEYIGLMTEWRFSRGVEEQTKAFLDGFSEVVPLQWLLYFDERELEVMLCGMQEIDLADWQRSTIYRHYNRSSKQISWFWQVVKEMDNEKRLRLLQFVTGTCRLPVGGFAELIGSNG---PQKFCIEKVGKET--WLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFG 930

HSP 2 Score: 60.8474 bits (146), Expect = 1.296e-9
Identity = 28/78 (35.90%), Postives = 43/78 (55.13%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR 898
            LP  +E++I  GG++YFV++N+    W  P    C+           LP GW++  ++ G  YF+DH  RTT + DPR
Sbjct:  467 LPSGWEKRIEQGGRMYFVNHNTRTTQWEDPRTQGCVLSEEP------LPDGWETRFTNEGVRYFVDHTMRTTTFQDPR 538

HSP 3 Score: 60.077 bits (144), Expect = 2.178e-9
Identity = 37/123 (30.08%), Postives = 54/123 (43.90%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVP------------------------KCLRKIGDFSC----LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPR----LLRALEAPDGYAT 937
            LP  +ER++   G+VY+VD+N+    W  P                           + L + G        LG LP GW+      GR+YF++H +RTTQ+ DPR    +L     PDG+ T
Sbjct:  392 LPPGWERRVDVRGRVYYVDHNTRTTTWQRPTTESVRNFEQWQSQRNQLQGAMQLFNQRFLFQTGGLPTENDPLGALPSGWEKRIEQGGRMYFVNHNTRTTQWEDPRTQGCVLSEEPLPDGWET 514

HSP 4 Score: 50.0618 bits (118), Expect = 2.599e-6
Identity = 24/73 (32.88%), Postives = 36/73 (49.32%), Query Frame = 2

Query:  677 FERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDP 895
            +E++    G++YFVD+N+    W  P                 LPPGW+      GRVY++DH +RTT +  P
Sbjct:  364 WEQRADPHGRLYFVDHNTKTTTWERPQP---------------LPPGWERRVDVRGRVYYVDHNTRTTTWQRP 421

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: ube3c (ubiquitin protein ligase E3C [Source:ZFIN;Acc:ZDB-GENE-040426-1211])

HSP 1 Score: 171.785 bits (434), Expect = 5.381e-44
Identity = 113/370 (30.54%), Postives = 187/370 (50.54%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFT-----DEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLC--IVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLE--DFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGIS-EIKIEDWQKNTRLKHCL-ENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRN--ANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            +   R  I E+++  +S    PDLR+R+ +        DE G+D GG+ RE+L  L +T F+   G F T   N G LL  +P    +V +    +++F+GR++G A++    ++  F   F   +L     ++      LD  +Y +L++++    D  +L L F++     G+ ++++L   G+ IPVT  ++  YI    DY L+ +  +     ++G+  V+  + L   +E E +VLL+G    I ++D +  T        + P+IK FW VV  +T++Q+ KLL+FVT  SR PL GF+           +LF    + N  A+P  LP A TC N L LP +   + +H KL  AIE   GF
Sbjct:  733 VTIRRPYIYEDAYDKLSPENEPDLRRRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKTGFNPHQGFFRTT--NQG-LLYPNPEARLLVGDGFTRHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTTGDVDIHHLASLDPEVYRNLLFLKSYQGDVEELGLNFTVVNNELGEAQVVELKAGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLATVINLEWLRMFDEQELQVLLSGAQVPIDLQDLKNFTNYSGGYTADHPVIKVFWHVVEGFTDEQKRKLLKFVTSCSRPPLLGFK-----------ELFPAFCVHNGGADPERLPTASTCMNLLKLPEFLEEETMHGKLLYAIESAAGF 1088

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: ube3b (ubiquitin protein ligase E3B [Source:ZFIN;Acc:ZDB-GENE-060526-231])

HSP 1 Score: 164.466 bits (415), Expect = 1.006e-41
Identity = 118/389 (30.33%), Postives = 183/389 (47.04%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKF-----TDEEGLDYGGVAREWLYLLSETLFSCRHGLFE-TNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIF---YKSILNKQP--QLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTG-ISEIKIEDWQKNTRLKHCLE-NEPLIKWFWEVVTS-YTEQQRTKLLQFVTGSSRIPLSGFEGLRGSY-----------------GSDEPKLFTIKVIRNANPLS-LPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            I   R  +LE+ +  +SRL P  L+  + +KF      DE G+D  GV +E+L  + + +F     LF+ T GD     L  SP   V+E+HL    F+G+++G A++ G  +D  F   F    + +    P   +++   LD   Y +L  ++  + D ++L LTFS E+++ G+L   +LC  G+   VT+ +K  YI     + +  K  +Q      G   ++  + +   +  E + L+ G   EI  ED +K+T        N  +I W W+++T  +T Q+R   L+FVT  SR PL GF  L+ S+                 GS     FT   IR   P   LP + TCFN L LP Y     L DKL  A+    GF
Sbjct:  695 ITVRRSRMLEDGYEQLSRLPPNSLKGTIRVKFINDLGMDEAGIDQDGVFKEFLEEIIKKVFDPELNLFKITIGDER---LYPSPTSYVHENHLQLMEFVGKMLGKAVYEGIVVDVPFAPFFLSQVRGVHTSMPYSSIDELPSLDAEFYKNLTSIKRYDGDVSELGLTFSYEEDIMGKLVCYELCPGGKTTLVTNENKISYIHMLAHHRMYVKIKKQTGAFIHGFKSLISPEWMGMFSTPEMQRLIAGDTGEIDFEDLRKHTVYYGGFHNNHRVIIWLWDILTKEFTAQERAMFLKFVTSCSRPPLLGFAHLKPSFSIRCVEVADDQDTGDTLGSVLRGFFT---IRKKEPAGRLPTSSTCFNLLKLPNYSKKSVLRDKLRYAVSMNTGF 1077

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: RSP5 (NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome [Source:SGD;Acc:S000000927])

HSP 1 Score: 331.643 bits (849), Expect = 1.460e-100
Identity = 159/360 (44.17%), Postives = 241/360 (66.94%), Query Frame = 2

Query: 1394 CIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            C I+  R NI E++++ I R  P DL+KRL++KF  EEGLDYGGV+RE+ +LLS  +F+  + LFE +   D   ++++P   +N +HL+YF FIGRV+G+ +FH  ++DA F    YK +L K+  L+D E +D  +YNSL WM +N+ID   L+LTFS + E FG++  +DL  +GR I VTD +K +Y+E Y  + + ++  EQ++    G  E++P+ ++   +E E E+L+ GI+EI IEDW+K+T  +   E++ +I+WFW+ V+ +  +QR +LLQF TG+SRIP++GF+ L+   GSD P+ FTI+  +      LPK+HTCFNR+DLP Y  Y  +  KLT A+EET GF 
Sbjct:  455 CHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSA-YDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGV-LDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQ---GSDGPRRFTIE--KAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFG 807

HSP 2 Score: 68.5514 bits (166), Expect = 3.075e-12
Identity = 33/93 (35.48%), Postives = 52/93 (55.91%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGD---------FSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALE 916
            LP  +E++ +  G+ YFVD+N+    W  P   + +R  G           S LG LP GW+   +++ RVYF+DH ++TT + DPRL  +L+
Sbjct:  333 LPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLD 425

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: TOM1 (E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase [Source:SGD;Acc:S000002865])

HSP 1 Score: 225.713 bits (574), Expect = 5.023e-61
Identity = 123/355 (34.65%), Postives = 202/355 (56.90%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLR-KRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRN-ANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEE 2458
            I   R+ +  +S+R +      +++  +L + F  E G+D GGV REW  +LS  +F+  + LF     +D      +    +N +HL +F FIG +IG AI    ++D  F +  YK+IL +   L+D E LD   Y SLVW+ +N+I D  +  TFS+E + +G+ ++I+L E G+ I VT+ +K  Y+++ V+Y L     EQ +    G Y ++ + ++   +E E E+L++G+ +I ++DW+ NT   +       + +FW  V S+  ++R KLLQFVTG+S++PL+GF+ L G  G     +    + R+  +   LP +HTCFN+L+LPPY SY+ L   L  AI E
Sbjct: 2913 ITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALF-LPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDI-IEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNG-----VCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINE 3260

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: HUL5 (Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock [Source:SGD;Acc:S000003109])

HSP 1 Score: 150.599 bits (379), Expect = 1.067e-37
Identity = 110/376 (29.26%), Postives = 180/376 (47.87%), Query Frame = 2

Query: 1379 MQKKLCIIETSRDNILEESFR---NISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFS-CRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQ----PQLEDFEELDKSIYNSLVWMRDNNIDD-ADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISE-IKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNAN--PLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            M+K+  II  SRDN+LE++F    +I       L    + +F +E G+D GG+ +E+L  +S+  F   +H LF TN     +   + P  + +   L Y  F+G+V+G  ++    ID  F   F K +LN          D    D  +YN+L+ + +   D+   L+LTF I+ E     +++DL  NG    VT ++   Y+ +  DY L+++C +       G+  ++    ++  N  E ++L++G  + I ++D + NT      E +  I  FWEV+  +  +++   L+FVT   + PL GF+ L   +G           IRNA      LP A TC N L LP Y +   L +KL  AI     F
Sbjct:  551 MRKQSAII--SRDNVLEDAFNAFNSIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRTN-----DRYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLSSFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEID-EPESSAKVVDLIPNGSKTYVTKDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDNIDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKALDPKFG-----------IRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARF 907

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: HUL4 (Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex [Source:SGD;Acc:S000003797])

HSP 1 Score: 149.828 bits (377), Expect = 2.175e-37
Identity = 110/380 (28.95%), Postives = 187/380 (49.21%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCI----VNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWM---RDNNIDDADLNLTFSI----------EQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQ-QILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCL----ENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I+  RD I  +S R I   +  DL K L ++F +E G+D GG+ +EW +LL+++LF+  +GLF    ++  +   + P            L  ++  G V+G+AIF+   +D  F K  YK + ++    ED+ EL      +L+ M    ++N +D   +LTF            + +   +   ++LCENGR +P+T ++K +++ ++V++ L +    QY +   G   V  +   +   N +E E L+ G  E    D++    +   +    ++   + WFWE++ S+    + KLLQFVT S RIP +G   +           F I ++ + +   LP AHTCFN + L  Y S K L  KL  AI E+ G+ 
Sbjct:  523 IKVRRDVISHDSLRCIKEHQG-DLLKSLRIEFVNEPGIDAGGLRKEWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGLAIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNLIKMLNYTEDNFEDV-FSLTFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIEPQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSDDSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIP----------FKISLLGSHDSDDLPLAHTCFNEICLWNYSSKKKLELKLLWAINESEGYG 890

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Match: UFD4 (Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000001493])

HSP 1 Score: 75.8702 bits (185), Expect = 2.555e-14
Identity = 102/418 (24.40%), Postives = 180/418 (43.06%), Query Frame = 2

Query: 1349 RDLRS-----KLKELMQKKLCIIETSRDNILEESFRNISRL-KPPDLRKRLVLKFTDEEG------LDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGN------LLRVSPLCIV--NEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYK-----SILNKQPQLEDFEE-------LDKSIYNSLVWM---RDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQ-----KNTRLKHCLE-NEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLS----LPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCG 2467
            +DLR+     +L  + ++KL I   SR  I     + +S+    PD+   L +++ +E G      L++  V  ++    S  ++ C    + +  D D        LL   PL     NE  +  F ++G  +  ++     +D  F K+F++     S  N      D E        +D  +  SL ++   +D+N+    L+LTF++        + I+L   G    +  ++  +YI   +D  L +   +Q +   +G  +V   + +  L  DE  V + G  E   EDW       N   +H    +  +I  F  +++++ + +R   LQF+TGS ++P+ GF+ L        PK FT+ +    + L+    LP   TC N L LP Y S   +  +L  AIEE  G
Sbjct: 1084 KDLRNDEALQQLGRITRRKLRI---SRKTIFATGLKILSKYGSSPDV---LEIEYQEEAGTGLGPTLEFYSVVSKYFARKSLNMWRCNSYSYRSEMDVDTTDDYITTLLFPEPLNPFSNNEKVIELFGYLGTFVARSLLDNRILDFRFSKVFFELLHRMSTPNVTTVPSDVETCLLMIELVDPLLAKSLKYIVANKDDNMTLESLSLTFTVPGN-----DDIELIPGGCNKSLNSSNVEEYIHGVIDQILGKGIEKQLKAFIEGFSKVFSYERMLILFPDEL-VDIFGRVE---EDWSMATLYTNLNAEHGYTMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLN-------PK-FTVVLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAG 1478

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49327 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD5])

HSP 1 Score: 321.242 bits (822), Expect = 1.424e-101
Identity = 161/358 (44.97%), Postives = 229/358 (63.97%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I  SR N+ EESF+ + R +P DLR+RL + F  EEGLDYGGVAREW +LLS  + +  + LFE    N+ +L +++    VN DHL YF FIGR I MA++HG +ID  F   FYK +LNK+  ++D E +D   YNSLVW+++NNI++  L + F+++ E+ G++   DL   G  I VT+ +K +YI    ++ L+    EQ     +G++EVLP   +   +E E E++L G+ EI +EDWQ+NT  +H   N   + WFW+ V +Y  ++R +LLQFVTG+ R+P+ GF  L G  G   P+ F I+ +       LP++HTCFNRLDLPPY SY+ L +KLT AIEET GFA
Sbjct:   11 ITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSL-QINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVTEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGDNG---PQKFCIEKV--GKETWLPRSHTCFNRLDLPPYKSYEQLVEKLTFAIEETEGFA 362

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49328 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD7])

HSP 1 Score: 303.523 bits (776), Expect = 2.979e-91
Identity = 189/590 (32.03%), Postives = 292/590 (49.49%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILD------MELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLC---------IIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFW-EVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCF 2386
            LP  +E+++   G++Y+VD+NS    W  P                 LP GW+      GR Y++DH +RTT +  P            A  IR  +N     +NQ+      ER +  +R     + ++ +       L +  E   ++      +  N R+ +  + P     A  D       E+  +N      +D N             + P  N     K       R  R K+ +   + LC          I  SR N+ EESF+ + R +P DLR+RL + F  EEGLDYGGVAREW +LLS  + +  + LFE    N+ +L +++    VN DHL YF FIGR I MA++HG +ID  F   FYK +LNK+  ++D E +D   YNSLVW+++NNI++  L + F+++ E+ G++   DL   G  I V + +K +YI    ++ L+    EQ     +G++EVLP   +   +E E E++L G+ EI +EDWQ+NT  +H   N   + WFW + V +Y  ++R +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ +       LP++HT +
Sbjct:  112 LPPGWEQRVDPHGRIYYVDHNSRTTAWERPQP---------------LPHGWERRTDSRGRTYYVDHNTRTTTWQRPT-----------AETIRTFEN----WQNQNRAMLGAERQQFQQRFLLPNQQDAERDNGPLGPLPAGWEKRVETNGRVYFVNHNTRT-TQWEDPRTQTTASADKPLPQGWEMRYTNEGIPYFVDHNTRTTTF-------TDPRGNGAPGVKGAAPAFERSFRWKVGQF--RHLCQANTLPSHIKITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSL-QINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWVKENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVIEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYRHYTRNSKQVMWFWQQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGSNG---PQKFCIEKV--GKETWLPRSHTWW 655

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO49383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG94])

HSP 1 Score: 277.715 bits (709), Expect = 1.661e-85
Identity = 139/336 (41.37%), Postives = 216/336 (64.29%), Query Frame = 2

Query: 1472 RKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCL-ENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            R RL ++F  EEGLDYGG ARE+ +L+S  +F+  +GLFE +  ND   ++VSP+ +  ++   +F F GR++G+ I H + +DA F + FYK++L     L D E LD   + S+ W+ +N+I+D  L+LTFS+ +E+FGQ+   +L   G+ I VT+ +K  Y+   V + +    +EQ E I +G  EV+   +++  +  E E++++G ++I I+DW++NT  +    +N  ++KWFW+ V+S+  +QR +LLQFVTG+S IP  GF  LRGS G   P+ F+I+  R  +   LP+AHTCFNRLD+P Y SY  L +KLT A+EET  FA+
Sbjct:   10 RNRLEIQFAGEEGLDYGGPAREFFFLISRQIFNPYYGLFEYSA-NDTYTVQVSPVSLYVDNSHEWFRFCGRIVGLVIIHQHLLDAFFTRTFYKALLRSLCDLSDVEALDALFHQSMTWVIENDIEDV-LDLTFSVSEEIFGQVTERELIPGGKDIAVTEQNKGDYVAEMVKWRVERGVSEQMESIVRGFNEVIDPALVNIFDARELELVISGTADIDIKDWRRNTEYRSGYHDNHKVVKWFWKAVSSFDNEQRLRLLQFVTGTSSIPYEGFAALRGSTG---PRKFSIE--RWGDYTKLPRAHTCFNRLDMPIYRSYDELLEKLTYAVEETGSFAM 338

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO46071 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR93])

HSP 1 Score: 289.271 bits (739), Expect = 2.416e-83
Identity = 155/363 (42.70%), Postives = 227/363 (62.53%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNI--SRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSP---LCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPL-IKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            I   R N++E+S+R +  S +KP  L+ RL + F  E GLDYGG+ REW YLLS+ +F+  +GLFE +  +D   L+++P   LC  NE+HL YF FIGRV GMA++HG  +DA F + FYK +L +   L D E +D   YNSL W+ +N  D  DL+L F +++E+FG L + DL  NG    VT+ +K +YI   + +    +  +Q     +G  +++P  ++   +E E E+L+ G+ EI   DW+KN+  +    ++ + I+WFW+ V S+  + R +LLQFVTG+SR+P++GF  L   YGS+ P+ FTI+      P SLP+AHTCFNRLDLP Y SY  L ++L  AIE T GF
Sbjct:  633 IHIRRRNLMEDSYRAVQLSIVKPEILKSRLWIVFDGETGLDYGGLQREWFYLLSKEVFNPYYGLFEYSA-SDNYTLQINPNSGLC--NEEHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLGRPITLIDMESVDSEYYNSLNWILEN--DPEDLDLHFCVDEELFGILSVKDLKPNGSQTNVTNENKREYINLVIKWRFVSRVEDQMRAFMEGFCDLIPHNLIQIFDERELELLMCGLGEIDTVDWRKNSNYRGEYHDKHIVIQWFWKAVNSFDIETRARLLQFVTGTSRVPMNGFSEL---YGSNGPQRFTIEPW--GTPHSLPRAHTCFNRLDLPRYRSYYELRERLRIAIENTQGF 985

HSP 2 Score: 56.9954 bits (136), Expect = 3.616e-8
Identity = 28/87 (32.18%), Postives = 45/87 (51.72%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--------LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            LP  +  + +  G+++F+D+NS    W+ P +    +               LG LP GW+      GRV++IDH +R+TQ+ DPRL
Sbjct:  509 LPPGWAVQRAPNGRLFFIDHNSRTTTWHDPRLTHLSQSPPSALPPSAPPAQDLGPLPDGWEERIHSDGRVFYIDHETRSTQWEDPRL 595

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO34592 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNX9])

HSP 1 Score: 194.897 bits (494), Expect = 4.197e-51
Identity = 119/369 (32.25%), Postives = 187/369 (50.68%), Query Frame = 2

Query: 1397 IIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLF------ETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDAD--LNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLK-HCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            +I+  RD+I++++   I+   P DL+K L + F +EE +D GGV +E+  LL   +   ++G+F      +T   ND      SP+          F  IG + G+AI++    D  F  + YK +L K+P L+DF     S+  +L ++ D + DD +    L F I +E +G LE IDL   G  +PVT +++ QY+E Y+DY LS+   +Q+E    G + V    +L+  +  E   ++ G  E   E  +K T  K       P+I  FW V   +    + K L F+TGS R+P+ G E +         K+    V    +   LP AHTCFN LDLP YP+ + +  KL+ A+E + GF +
Sbjct:  707 VIKVRRDSIVQDTLTQITTQSPYDLKKPLKVIFANEEAVDAGGVKKEFFLLLLTEILDPKYGMFTYLEDSQTIWFNDLTF-EESPM----------FLLIGVICGLAIYNSTIFDLRFPPVLYKKLLRKKPNLDDFRGFQPSVARNLQYLLDYSDDDLEDTFGLNFQIMREKYGALEYIDLVPGGGEVPVTQDNREQYVELYIDYYLSKAVDKQFEAFFTGFHRVCGGHVLEFFHPQELMEMVVGCQEYDFEVLEKITEYKGEYYRKHPVIANFWTVFYEFPLSMKKKFLAFLTGSDRVPILGMESM---------KMIIQPVAGGEHGDHLPVAHTCFNLLDLPRYPTLEMMRTKLSQAVEFSKGFGL 1055

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: smurf2 (E3 ubiquitin-protein ligase SMURF2 [Source:NCBI gene;Acc:101160323])

HSP 1 Score: 412.535 bits (1059), Expect = 1.587e-130
Identity = 277/835 (33.17%), Postives = 428/835 (51.26%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHI------NGSSRGLTAENSQQSL----RYSGNQSSQRNSKEHSFSN-------DENN--SRPGPSQISHGDLLLPINFERKISDGG-KVYFVDNNSGAIHWY-HPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALE---------APDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + ++L +  A  L K+   + PDPFA+++ +    C  T+    T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L + +   S  VRG + + +   D   I T   +     +      +G     TA    Q L    R +  +   R + E+S          DEN   S  G +  +   +L P N ++++ +   +     N     H + HP +P+                G++   +  G+VYF+   +  + + DPR+ R L           P G+   IR          + +   T+    R            S+   +  N   + +  NN+S N +   Q   ++Q +   PS                              LP +   ++ P     L+ K      L+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ DN+I    L+ TF +E   +G++   +L  NG+ IPVT  +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A+  +LPKAHTCFNR+D+PPY +Y  L+DKL  AIEETCGFAV
Sbjct:   12 VKLRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQ--HYDLYIGKSDSITISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLNKLSPNDSDTVRGQIVLSLQSRD--RIGTGGPVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCIVDENTPVSTNGATPTT---VLPPSNGDQRVQERRVRSQRHRNYMSRTHLHTHPDLPE----------------GYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWE--IRNTATGRVYFVDHNNRTTQFTDPRL-----------SANLHLVLNPSSNGSRGNNESQNVSRQNQMKDQAQQALVSPS-----------------------------QLPEEAECLTVPKYKRDLVQK------LKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTQETKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIHDWKGNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DASTNNLPKAHTCFNRIDIPPYETYDKLYDKLLTAIEETCGFAV 769

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: SMURF2 (E3 ubiquitin-protein ligase SMURF2 [Source:NCBI gene;Acc:101159489])

HSP 1 Score: 412.149 bits (1058), Expect = 3.823e-130
Identity = 280/826 (33.90%), Postives = 426/826 (51.57%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVY-FIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETI-QTNRRSQSSGQIPSFNNQAILD----------MELSMSNISEDKN--------IDCNNELAP--LPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + V+L +  A  L K+   + PDPFA+++ +    C  T+    T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D       +     +  N    G   E    S R        R ++    +      R G  +  +GD   P +     S       VD N+  +   + AV  C             P G   +Q    R     +++SRT  ++ P L      P+GY     + Q     +  Q+G  T     R  R  SN    E          L    EI N +T     +   NR +Q +    S N   +L+          M+   +N+S +++        +     L+P  LP +   ++ P     L+ K      L+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++G+A+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+  N+I    L+ TF +E   +G++   +L  NG+ I VT+++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V S+ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +AN  +LPKAHTCFNR+D+PPY SY+ L+DKL  AIEETCGFAV
Sbjct:   12 VKVRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGK-ADSITISVWNHKKIHKKQGAGFLGCVRLLSNTINRLKDTGYQRLDLNKLGPNDNDTVRGQIVVSLQSRDRIGTGGPVVDCSRLFDNDLPDGW-EERRTTSGRIQYLNHITRTTQWERPTRCPEAVRDG-EETGNGDKYRPASEYSSPSGRPSSCVVDENTPVMTPVNGAVASC-------------PLGEQRVQERRVRSQRHRNYMSRTHLHTPPDL------PEGYEQRTTQ-QGQVYFLHTQTGVST-WHDPRVPRDLSNVNCEE-------LGPLLPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNSPSPNGSRVAMDSQNTNLSSNQSQLKDQGGPVGPPQALSPAQLPEEAECLTVPKYKRDLVQK------LKILRQELSQHQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGLAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILGNDITGV-LDHTFCVEHSAYGEIIPHELKPNGKSISVTEDTKKEYVRLYVNWRFLHGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIVDWRSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DANANNLPKAHTCFNRIDIPPYESYEKLYDKLLTAIEETCGFAV 794

[Close]

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVY-FIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETI-QTNRRSQSSGQIPSFNNQAILD----------MELSMSNISEDKN--------IDCNNELAP--LPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            + V+L +  A  L K+   + PDPFA+++ +    C  T+    T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +   D       +     +  N    G   E    S R        R ++    +      R G  +  +GD   P +     S       VD N+  +   + AV  C             P G   +Q    R     +++SRT  ++ P L      P+GY     + Q     +  Q+G  T     R  R  SN    E          L    EI N +T     +   NR +Q +    S N   +L+          M+   +N+S +++        +     L+P  LP +   ++ P     L+ K      L+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++G+A+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+  N+I    L+ TF +E   +G++   +L  NG+ I VT+++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V S+ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +AN  +LPKAHTCFNR+D+PPY SY+ L+DKL  AIEETCGFAV
Sbjct:   12 VKVRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGK-ADSITISVWNHKKIHKKQGAGFLGCVRLLSNTINRLKDTGYQRLDLNKLGPNDNDTVRGQIVVSLQSRDRIGTGGPVVDCSRLFDNDLPDGW-EERRTTSGRIQYLNHITRTTQWERPTRCPEAVRDG-EETGNGDKYRPASEYSSPSGRPSSCVVDENTPVMTPVNGAVASC-------------PLGEQRVQERRVRSQRHRNYMSRTHLHTPPDL------PEGYEQRTTQ-QGQVYFLHTQTGVST-WHDPRVPRDLSNVNCEE-------LGPLLPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNSPSPNGSRVAMDSQNTNLSSNQSQLKDQGGPVGPPQALSPAQLPEEAECLTVPKYKRDLVQK------LKILRQELSQHQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGLAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILGNDITGV-LDHTFCVEHSAYGEIIPHELKPNGKSISVTEDTKKEYVRLYVNWRFLHGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIVDWRSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DANANNLPKAHTCFNRIDIPPYESYEKLYDKLLTAIEETCGFAV 794

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: SMURF2 (E3 ubiquitin-protein ligase SMURF2 [Source:NCBI gene;Acc:101159489])

HSP 1 Score: 392.504 bits (1007), Expect = 7.006e-123
Identity = 235/628 (37.42%), Postives = 347/628 (55.25%), Query Frame = 2

Query:  806 LPPGWDSLQSDSGRVYFIDHISRTTQYSDP-RLLRALEAPDGYATVIRKLQNS-------GAVIKNQSGYYTKIERS-RSDRRRS--------------NGTKLESSQTAVTFNL----------------------------LESSAEINNQSTNHTETI-QTNRRSQSSGQIPSFNNQAILD----------MELSMSNISEDKN--------IDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            LP GW+  ++ SGR+ +++HI+RTTQ+  P R      +P G  +     +N+       GAV     G     ER  RS R R+               G +  ++Q    + L                            L    EI N +T     +   NR +Q +    S N   +L+          M+   +N+S +++        +     L+P  L           Y    K ++  KL+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++G+A+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+  N+I    L+ TF +E   +G++   +L  NG+ I VT+++K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V S+ E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +AN  +LPKAHTCFNR+D+PPY SY+ L+DKL  AIEETCGFAV
Sbjct:  159 LPDGWEERRTTSGRIQYLNHITRTTQWERPTRPASEYSSPSGRPSSCVVDENTPVMTPVNGAVASCPLGEQRVQERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLLPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNSPSPNGSRVAMDSQNTNLSSNQSQLKDQGGPVGPPQALSPAQLPEEAECLTVPKY----KRDLVQKLKILRQELSQHQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGLAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILGNDITGV-LDHTFCVEHSAYGEIIPHELKPNGKSISVTEDTKKEYVRLYVNWRFLHGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIVDWRSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DANANNLPKAHTCFNRIDIPPYESYEKLYDKLLTAIEETCGFAV 776

HSP 2 Score: 76.2554 bits (186), Expect = 1.046e-13
Identity = 35/81 (43.21%), Postives = 53/81 (65.43%), Query Frame = 2

Query:  665 LPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            LP  +E++ +  G+VYF+   +G   W+ P VP   R + + +C  LG L PGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:  256 LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNVNCEELGPLLPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 333

HSP 3 Score: 62.003 bits (149), Expect = 2.328e-9
Identity = 35/126 (27.78%), Postives = 64/126 (50.79%), Query Frame = 2

Query:   86 ITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEM 445
            + V+L +  A  L K+   + PDPFA+++ +    C  T+    T +P WN+ + + +    D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L +     +  VRG + V +
Sbjct:   12 VKVRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGK-ADSITISVWNHKKIHKKQGAGFLGCVRLLSNTINRLKDTGYQRLDLNKLGPNDNDTVRGQIVVSL 136

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: smurf2 (E3 ubiquitin-protein ligase SMURF2 [Source:NCBI gene;Acc:101160323])

HSP 1 Score: 381.333 bits (978), Expect = 2.262e-119
Identity = 186/385 (48.31%), Postives = 264/385 (68.57%), Query Frame = 2

Query: 1325 KNNIEIKLRDLRSKLKELM-QKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            K ++  KL+ LR +L +   Q   C IE SR+ I EES+R + +++P DL KRL++KF  EEGLDYGGVAREWLYLLS  + +  +GLF+ + D D   L+++P   VN +HL YFHF+GR++GMA+FHG+YID  F   FYK +L K   L+D E +D  ++NSLVW+ DN+I    L+ TF +E   +G++   +L  NG+ IPVT  +K +Y+  YV++        Q+  ++KG  EV+PQ +L   +E E E+++ G+ +I I DW+ NTRLKHC  +  ++KWFW+ V  + E++R +LLQFVTGSSR+PL GF+ L+G+ G   P+LFTI  I +A+  +LPKAHTCFNR+D+PPY +Y  L+DKL  AIEETCGFAV
Sbjct:  331 KRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRD-DIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTQETKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDIHDWKGNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAG---PRLFTIHQI-DASTNNLPKAHTCFNRIDIPPYETYDKLYDKLLTAIEETCGFAV 709

HSP 2 Score: 112.849 bits (281), Expect = 3.718e-25
Identity = 87/294 (29.59%), Postives = 140/294 (47.62%), Query Frame = 2

Query:  113 AHKLIKRSLLKNPDPFAQIIYNNHSFC--TNAQEKTTNPAWNKSFHIELHSIK-DKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDR----GERRLQLARNNAFPSKPVRGFVKVEMIK----------IDSHSIPTNLSMPQIIHINGSSRG-----LTAENSQQSLRYSG-------NQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSC--LGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRL 901
            A  L K+   + PDPFA+++ +    C  T+    T +P WN+  H +L+  K D + I +WN +   +K+ + FLG   L ++ I+R    G +RL L + +   S  VRG + + +            +D   +  N  +P       SS G     +  EN+  S   +        +   QR  +    S    N        +H DL  P  +E++ +  G+VYF+   +G   W+ P VP   R + + +C  LG LPPGW+   + +GRVYF+DH +RTTQ++DPRL
Sbjct:   22 AKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQ--HYDLYIGKSDSITISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLNKLSPNDSDTVRGQIVLSLQSRDRIGTGGPVVDCSRLFDN-DLPDGPASEYSSPGRPLSCIVDENTPVSTNGATPTTVLPPSNGDQRVQERRVRSQRHRNYMSRTHLHTHPDL--PEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVP---RDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 307

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000030063.1 (SMESG000030063.1)

HSP 1 Score: 1660.2 bits (4298), Expect = 0.000e+0
Identity = 802/802 (100.00%), Postives = 802/802 (100.00%), Query Frame = 2

Query:   74 MSDSITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDRGERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAVM 2479
            MSDSITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDRGERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAVM
Sbjct:    1 MSDSITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDRGERRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAVM 802

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000008708.1 (SMESG000008708.1)

HSP 1 Score: 317.775 bits (813), Expect = 2.832e-95
Identity = 158/358 (44.13%), Postives = 232/358 (64.80%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            I   R+ ++E+SF  I R+KP DL+KRL ++F DE+GLDYGG++REW + LS  L +  + LFE   DN+ +L +++P   +N +HL YF F+GR + MA+FH  +ID+ F   FYK +L K+  L+D E +D   YNSL ++RDNNID+ D+ L FS E E+FG++E  +L  NGR I VT+ +K++YI+  V++  S    EQ +    G  E++P   L   +E + E+LL G+ +I ++DW+ N+  +   ++ P IKWFWE V      ++ +LLQFVTG+ R+P+ GF  L GS G   P+ F I+ I   +   LP++HTCFNRLDLPPY SY+ L +KLT A+ ET GF 
Sbjct:  392 IIVRRNMLMEDSFNEIMRVKPKDLQKRLFIQFKDEDGLDYGGISREWFFQLSHELLNPMYCLFEYASDNNYSL-QINPASFINPEHLQYFRFVGRFVAMAMFHRKFIDSGFTIPFYKKMLGKRITLQDIETVDVEYYNSLKFIRDNNIDECDMGLVFSAEYELFGKIEEQELKTNGRDIEVTEENKNEYIDLMVNWRFSRGVVEQTKAFMDGFNEIVPLHWLQVYDERQIELLLCGMQQIDVKDWEANSIYRVYTKDSPQIKWFWEFVGKLPNDEKIRLLQFVTGTCRLPVGGFRELMGSNG---PQKFCIEKIGKES--WLPRSHTCFNRLDLPPYASYEQLKEKLTYAVMETEGFG 743

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000039114.1 (SMESG000039114.1)

HSP 1 Score: 306.99 bits (785), Expect = 1.954e-91
Identity = 159/359 (44.29%), Postives = 231/359 (64.35%), Query Frame = 2

Query: 1400 IETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFAV 2476
            I  SR ++L +SF  I +++P  LRKRL + F  EEGLDYGGVAREW + LS+ L +    LFE   DN+ +L +++P   VN +HL YF F+GR I MA+FHG +ID+ F   FYK +LNK+P LED E +D   YNSL ++++ ++++ DL L FS + E+ G+LE  +L   G  I VT+++K +YI+  V++  +     Q E   KG  EVLP   L   +E E E+LL G+ +I ++DW+K+T  +H  +N   I  FW+ V + T + R +LLQFVTG+ R+P+ GF+ L GS G   P+ F I+ +       LP++HTCFNRLDLPPY S++ L +KL  AI+ET GF++
Sbjct:  385 INVSRKDLLTDSFNQIMQIQPTHLRKRLYITFKGEEGLDYGGVAREWFFRLSQELLNPMICLFEYACDNNYSL-KINPASFVNPEHLQYFRFVGRFIAMALFHGKFIDSGFTLSFYKRMLNKKPILEDIEAVDYLYYNSLKFIKEMDLNECDLELYFSADFEILGKLETQELKPGGHSILVTEDNKEEYIDLMVNWRFNRGIENQAESFIKGFNEVLPLNWLYYFDEKEIELLLCGMQDINVDDWEKHTVYRHYTQNSKQIIHFWKYVRNLTNEMRVRLLQFVTGTCRLPIGGFKNLMGSNG---PQKFCIEKV--GKETWLPRSHTCFNRLDLPPYKSFQQLSEKLNYAIKETEGFSM 737

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000038772.1 (SMESG000038772.1)

HSP 1 Score: 291.967 bits (746), Expect = 3.468e-86
Identity = 153/377 (40.58%), Postives = 230/377 (61.01%), Query Frame = 2

Query: 1343 KLRDLRSKLKELMQKKLCIIETSRDNILEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA 2473
            +L   + + K +M      I   RD++L+ SF  I  L   +L++RL ++F  EEGLDYGGVA+EW   LS  + +  + LFE     + +L +++P   VN +HL YF FIGR IGMA FHG  ID  F   FYK ILNK   L+D E +D   Y SL+++++N I+D DL L FS   EV G+++L +L   G  + VTD +K +YI+  + + L++   +Q +   +G+YE++P  +L   +E E E+LL G+ +I ++DW+++T  +    +   + WFW+ V S   +Q+ +LLQFVTG+ R+PL GF  L+G  G    +LF ++  R  +   LP++HTCFNRLDLPPY +Y+   +KLT A EET GF 
Sbjct:  335 RLEAFQDECKSMMPTGTVKINFKRDDLLDSSFNQIINLSTTELKQRLSIQFLGEEGLDYGGVAKEWFLRLSHEILNPMYCLFEYANTYNYSL-KINPGSFVNPNHLSYFKFIGRFIGMAFFHGRCIDNGFTIPFYKQILNKPLTLQDIESIDLEFYQSLLFIKENPINDCDLELYFSASYEVLGEVKLHELIPGGNEVKVTDENKKEYIQLMIPWWLTKGVEKQMKNFLEGLYELVPLSLLQRFDERELELLLCGLQKIDVDDWERHTVYRQYSPSNIQVIWFWKFVRSLDHEQQARLLQFVTGTCRLPLGGFSELQGGNGR---QLFCLE--RVGDETWLPRSHTCFNRLDLPPYKTYEQFIEKLTLAFEETEGFG 705

[Close]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000013095.1 (SMESG000013095.1)

HSP 1 Score: 288.5 bits (737), Expect = 1.465e-83
Identity = 250/864 (28.94%), Postives = 413/864 (47.80%), Query Frame = 2

Query:   92 VKLKINSAHKLIKRSLLKNPDPFAQI-IYNN--------HSFCTNAQEKTTNPAWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSA--FLG------------SAVLTADFIDRGER-RLQLARNNAFPSKPVRG----FVKVEMIKID-----------SHSIPTNLSMPQI-IHINGSSRGLTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPINFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWDSLQSDSGRVYFIDHISRTTQYSDPRLL---RALEAPDGYATVIRKLQNSGAVIKNQSGYYTKIERSRSDRRRSNGTKLESS-----------QTAVTFNLLESSAEINNQSTNHTETIQTNRRSQSSGQIP--------SFNNQAILDMELSMSNISEDKNID-----CNNELAPLPLQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCI-IETSRDNILEESFRNISRLKPPDLRK-RLVLKFTDEEGLDYGGVAREWLYLLSETLFSCRHGLFETNGDNDGNLLRVSPLC-IVNEDHLHYFHFIGRVIGMAIFHGYYIDADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFSIEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQIKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLE-NEPLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKLFTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGF 2470
            ++++I  A  L+K+ +    DP+ ++ +Y +         S  T   +KT NP WN+ F   ++  +++L I      +YD+ + +   FLG            +  +T+    +  + R + +R+N      V+G    ++    I ID            +SIP  ++  +I IH   +S   TAE          +Q+ +    +H  S      +P  +Q       LP  +E +    G+VY+VD+ +    W HPA     +K+   + +  +P   + L   S R         ++      +    R +   D  A+ IR +         Q+     I  S       N TK  +S           Q A+T N        N+++T   +      RS+S+   P        S  +  ++D+    +   E ++ D      N+       +  R+++ +   +  ++N  + K    RSKL+   +      +  +R NI E+S+R I  +K  ++ K +L ++F  E+GLDYGGV REW +LLS  +F+  +GLFE +   D   L+++PL  + NE+HL YF FIGRV GMA++HG  +D  F +  YK +L K+  L+D E +D   Y SL ++ +N  D A L+LTFS+++E FGQ   +DL  +GR   VT  +K QYIE  +++   ++   Q     +G  +V+    L   + +E E+LL G+ +I + DW+ NT  K     N P+I  FW+ V ++  + R++LLQFVTG+SR+P++GF  L GS G   P+ FTI+  +   P  LP+AHTCFNRLDLPPY +++ L  KL  AIE   GF
Sbjct:   35 LRVRIVGATNLMKKDIFGTSDPYCKLSLYKSIRSSTVVGSSVQTRTMKKTLNPRWNEEFLFRVNVNENRLVI-----EMYDENRLTRDDFLGVISIHLPQLEICTESVTSHLAPKSFKLRPRSSRSN------VKGNLILYLAYLPIDIDPASWTPGPTDEGNSIPGTVADSEITIHTEENS---TAEVLANGWEERVDQNGRIYYVDHK-SKRTQWEKPTNAQ-------LPEGWEERADLSGRVYYVDHANQKTQWEHPASKSAPQKVNKLAKIN-MPAANNPL---SVRAVSPTRPGPSSDVDAATMFLRRRQISHEDTVASRIRPVATESLPTVEQTTEPPPITASVE-----NITKELASITVDEELPPGWQMAMTTNGRRFFINHNDKTTTWEDPRAIRNRSKSTSGTPAPTAINRTSSRDDLLVDLGPLPAGWEEREHTDGRIFFVNHNTKTTQWEDPRLNKFSGPAVPYSRN-YKQKYDYFRSKLRAPREPNAKFEMRINRKNIFEDSYRLIYNVKKTEMLKYKLWIEFIGEKGLDYGGVQREWFFLLSREMFNPYYGLFEYSA-ADNYTLQINPLSGVANENHLRYFKFIGRVAGMAVYHGRLLDGFFIRPLYKMMLGKKISLKDMESVDTEYYRSLKFILEN--DPAVLDLTFSVDEEHFGQKVEVDLIPDGRSKAVTQENKRQYIECIINWRFVKRVESQMTAFLEGFQDVVTLNTLQIFDANELELLLCGLQDINVTDWKGNTNYKGEYNPNHPVIVNFWKTVYTFNNELRSRLLQFVTGTSRVPMNGFVELWGSTG---PQKFTIE--KWGTPDQLPRAHTCFNRLDLPPYVTFEELRRKLLIAIENAEGF 858

The following BLAST results are available for this feature:

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SMURF1	5.172e-135	33.66	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
SMURF1	5.172e-135	33.66	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
SMURF1	3.082e-120	47.14	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
SMURF1	3.082e-120	47.14	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
SMURF2	1.282e-119	37.30	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
wwp-1	1.330e-99	44.69	E3 ubiquitin-protein ligase [Source:UniProtKB/TrE...	[more]
wwp-1	1.330e-99	44.69	E3 ubiquitin-protein ligase [Source:UniProtKB/TrE...	[more]
wwp-1	1.498e-99	44.69	E3 ubiquitin-protein ligase [Source:UniProtKB/TrE...	[more]
wwp-1	1.498e-99	44.69	E3 ubiquitin-protein ligase [Source:UniProtKB/TrE...	[more]
wwp-1	6.959e-98	44.69	E3 ubiquitin-protein ligase [Source:UniProtKB/TrE...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Smurf	8.358e-118	48.28	gene:FBgn0029006 transcript:FBtr0086833	[more]
Smurf	8.358e-118	48.28	gene:FBgn0029006 transcript:FBtr0333168	[more]
Su(dx)	1.003e-100	34.04	gene:FBgn0003557 transcript:FBtr0077823	[more]
Su(dx)	1.003e-100	34.04	gene:FBgn0003557 transcript:FBtr0077824	[more]
Su(dx)	1.003e-100	34.04	gene:FBgn0003557 transcript:FBtr0339529	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
smurf2	1.270e-135	33.53	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
smurf1	5.149e-120	46.29	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
itcha	4.450e-107	34.93	itchy E3 ubiquitin protein ligase a [Source:ZFIN;A...	[more]
wwp1	2.098e-99	34.24	WW domain containing E3 ubiquitin protein ligase 1...	[more]
wwp1	7.227e-97	43.99	WW domain containing E3 ubiquitin protein ligase 1...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
crybb2	7.918e-120	45.73	crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-586...	[more]
crybb2	5.607e-119	46.70	crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-586...	[more]
crybb2	1.780e-118	46.70	crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-586...	[more]
crybb2	6.180e-116	46.45	crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-586...	[more]
crybb2	1.268e-115	46.45	crystallin beta B2 [Source:Xenbase;Acc:XB-GENE-586...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Smurf2	1.830e-120	36.61	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
Smurf2	3.020e-120	36.61	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
Smurf2	3.020e-120	36.61	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
Smurf1	1.076e-119	47.14	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
Smurf1	2.089e-119	47.14	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]

BLAST of E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|A9JRZ0\|SMUF2_DANRE	9.584e-135	33.53	E3 ubiquitin-protein ligase SMURF2 OS=Danio rerio ...	[more]
sp\|Q9HCE7\|SMUF1_HUMAN	2.484e-134	33.66	E3 ubiquitin-protein ligase SMURF1 OS=Homo sapiens...	[more]
sp\|Q2TAS2\|SMUF2_XENLA	1.268e-121	37.54	E3 ubiquitin-protein ligase SMURF2 OS=Xenopus laev...	[more]
sp\|A2A5Z6\|SMUF2_MOUSE	2.113e-119	36.61	E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus...	[more]
sp\|Q9HAU4\|SMUF2_HUMAN	6.157e-119	37.30	E3 ubiquitin-protein ligase SMURF2 OS=Homo sapiens...	[more]

BLAST of E3 ubiquitin-protein ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A087TNR4	5.407e-145	41.41	E3 ubiquitin-protein ligase SMURF2 (Fragment) OS=S...	[more]
A0A088A7D3	4.604e-142	40.30	E3 ubiquitin-protein ligase OS=Apis mellifera OX=7...	[more]
A0A0M9A0C8	4.433e-141	39.74	E3 ubiquitin-protein ligase SMURF2 (Fragment) OS=M...	[more]
W8BRF4	6.647e-141	40.13	E3 ubiquitin-protein ligase Smurf1 OS=Ceratitis ca...	[more]
E9GIQ7	1.103e-140	32.71	E3 ubiquitin-protein ligase OS=Daphnia pulex OX=66...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SMURF2	1.974e-122	36.83	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
smurf1	5.127e-121	48.18	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
smurf1	1.873e-119	47.92	SMAD specific E3 ubiquitin protein ligase 1 [Sourc...	[more]
SMURF2	8.217e-118	42.61	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
itcha	4.180e-104	35.10	E3 ubiquitin-protein ligase Itchy-like [Source:NCB...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
smurf2	6.927e-96	31.62	SMAD specific E3 ubiquitin protein ligase 2 [Sourc...	[more]
nedd4a	8.965e-94	44.20	NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;A...	[more]
wwp2	5.993e-77	43.35	WW domain containing E3 ubiquitin protein ligase 2...	[more]
ube3c	5.381e-44	30.54	ubiquitin protein ligase E3C [Source:ZFIN;Acc:ZDB-...	[more]
ube3b	1.006e-41	30.33	ubiquitin protein ligase E3B [Source:ZFIN;Acc:ZDB-...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
RSP5	1.460e-100	44.17	NEDD4 family E3 ubiquitin ligase; regulates proces...	[more]
TOM1	5.023e-61	34.65	E3 ubiquitin ligase of the hect-domain class; has ...	[more]
HUL5	1.067e-37	29.26	Multiubiquitin chain assembly factor (E4); proteas...	[more]
HUL4	2.175e-37	28.95	Protein with similarity to hect domain E3 ubiquiti...	[more]
UFD4	2.555e-14	24.40	Ubiquitin-protein ligase (E3); interacts with Rpt4...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO49327	1.424e-101	44.97	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49328	2.979e-91	32.03	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49383	1.661e-85	41.37	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46071	2.416e-83	42.70	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO34592	4.197e-51	32.25	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
smurf2	1.587e-130	33.17	E3 ubiquitin-protein ligase SMURF2 [Source:NCBI ge...	[more]
SMURF2	3.823e-130	33.90	E3 ubiquitin-protein ligase SMURF2 [Source:NCBI ge...	[more]
SMURF2	3.823e-130	33.90	E3 ubiquitin-protein ligase SMURF2 [Source:NCBI ge...	[more]
SMURF2	7.006e-123	37.42	E3 ubiquitin-protein ligase SMURF2 [Source:NCBI ge...	[more]
smurf2	2.262e-119	48.31	E3 ubiquitin-protein ligase SMURF2 [Source:NCBI ge...	[more]

BLAST of E3 ubiquitin-protein ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000030063.1	0.000e+0	100.00	SMESG000030063.1	[more]
SMESG000008708.1	2.832e-95	44.13	SMESG000008708.1	[more]
SMESG000039114.1	1.954e-91	44.29	SMESG000039114.1	[more]
SMESG000038772.1	3.468e-86	40.58	SMESG000038772.1	[more]
SMESG000013095.1	1.465e-83	28.94	SMESG000013095.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30032411 ID=SMED30032411|Name=E3 ubiquitin-protein ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=2849bp
ATTTAAAAAAGATAAAATTACAAATTAATAATTAATTATATTATGATAAA
ATTTGGTAATATTTCTTAGTATTATGTCAGACAGCATAACTGTTAAACTT
AAGATTAATTCGGCTCATAAGTTAATTAAAAGGTCACTTTTAAAAAATCC
TGATCCATTCGCTCAAATTATTTATAATAATCATAGCTTCTGTACAAATG
CTCAAGAAAAAACCACTAATCCAGCTTGGAATAAATCATTTCATATTGAA
TTGCATTCAATTAAAGATAAATTGGAAATAATCATCTGGAATCAAAGGGT
ATATGACCAGAAAAAATCATCCGCTTTTCTTGGCAGCGCTGTACTCACTG
CAGACTTTATTGATCGAGGAGAGCGTCGTTTGCAGTTAGCAAGAAACAAC
GCTTTTCCGTCAAAACCGGTTCGAGGTTTTGTAAAAGTGGAAATGATTAA
AATTGATAGTCATTCCATCCCTACAAATTTAAGTATGCCTCAAATTATCC
ATATTAACGGGAGTTCAAGAGGTTTAACCGCTGAAAATTCACAACAATCG
TTACGATATTCAGGAAATCAGTCTAGTCAAAGAAACAGCAAAGAGCATTC
TTTCTCGAATGACGAAAATAACTCACGCCCAGGACCGTCTCAAATAAGCC
ACGGAGACTTGTTGCTACCAATCAATTTCGAACGAAAAATTTCAGATGGA
GGCAAAGTGTATTTTGTGGATAATAATAGCGGTGCTATCCATTGGTATCA
TCCAGCTGTGCCAAAATGTCTTCGGAAAATAGGAGATTTTTCCTGCCTAG
GGTTGCTTCCTCCTGGCTGGGATTCCTTGCAAAGCGACTCCGGCCGAGTC
TATTTCATCGACCATATTTCACGAACCACTCAATATTCCGACCCGCGCCT
TCTAAGAGCTCTCGAGGCGCCCGATGGCTATGCAACAGTAATTAGAAAAC
TGCAAAATTCCGGAGCCGTAATCAAAAACCAAAGTGGGTATTACACAAAA
ATAGAAAGATCACGCAGCGACCGACGCAGATCCAACGGTACGAAACTTGA
GTCCAGTCAAACTGCCGTAACTTTTAATTTGTTGGAGAGCAGTGCGGAAA
TCAATAATCAGTCGACCAATCACACTGAGACAATTCAGACCAACAGACGA
TCGCAATCTTCCGGACAAATCCCATCGTTTAATAATCAAGCCATTTTAGA
CATGGAACTCAGTATGAGCAATATTAGTGAAGACAAAAATATTGATTGCA
ATAATGAATTAGCCCCCTTGCCTTTACAGAGACGTAGAATCAGCCGTCCC
ACATCTAATTATATTCTCATGAACAAAAATAACATTGAAATTAAACTACG
GGACTTACGATCGAAATTAAAGGAATTGATGCAAAAAAAGTTGTGTATAA
TCGAAACGAGTCGTGATAATATTTTAGAAGAGTCATTCCGAAATATTTCA
AGATTGAAACCTCCTGATTTGAGGAAAAGATTGGTTTTGAAATTCACCGA
CGAAGAAGGCCTTGACTATGGCGGAGTAGCGAGAGAGTGGTTATATTTAT
TATCAGAAACCTTATTCAGTTGTCGGCACGGTTTGTTTGAAACCAACGGA
GATAACGATGGGAATTTGCTTCGAGTCAGCCCTTTGTGCATAGTCAATGA
AGATCATTTGCACTATTTCCATTTCATAGGCCGTGTCATTGGAATGGCAA
TTTTTCACGGATATTATATCGATGCAGATTTCTGTAAAATATTTTACAAA
AGCATTCTTAACAAGCAACCACAGTTAGAGGATTTCGAAGAACTCGATAA
ATCAATTTATAATAGTTTGGTGTGGATGCGAGATAATAACATCGATGATG
CTGATCTCAATCTCACATTCTCTATCGAGCAGGAGGTTTTTGGCCAGCTC
GAACTCATTGACCTTTGTGAAAATGGCAGGTTCATCCCGGTCACGGACAA
TTCAAAAAGTCAATATATAGAACGCTATGTCGATTACAACCTGTCAGAGA
AATGTGCTGAGCAATATGAACAGATCAAGAAAGGAATTTATGAAGTGCTT
CCTCAACAAATCTTAGACAATCTCAATGAAGATGAATTTGAAGTTTTATT
AACGGGAATAAGTGAAATTAAAATTGAAGACTGGCAAAAAAATACCCGTT
TGAAGCATTGTTTAGAAAATGAACCGCTAATTAAATGGTTTTGGGAAGTT
GTTACTTCTTATACCGAACAACAGCGGACCAAATTACTACAATTCGTCAC
CGGTTCTTCACGAATTCCGTTATCTGGATTTGAAGGTCTTCGAGGTAGTT
ATGGCAGTGATGAACCGAAATTGTTTACAATAAAAGTCATTCGGAACGCT
AATCCACTGAGTCTTCCAAAAGCGCACACTTGTTTCAATCGTCTCGATCT
GCCACCATATCCATCATACAAATGTCTCCATGACAAATTGACATGTGCTA
TCGAAGAGACTTGTGGATTTGCTGTCATGTGAAATGACGATTTGACATTG
ACATATTTCTGACTTGCTCTGTGCTGCCTTGGATTTTAAACAGAATCTCC
TAATTTGTGAATATAGATATTTTTATTAAGATATTATTACAGTCAACCTT
GTTGCCGAGACTCCTGGAATCATAGAATCGAGTATTTCTGGGCATATATG
ATGTTACAATATATATTTATACATAAAATTCGTAGCAGTTTTTCATATTT
TTTTCTATCGACAGTTGGAAATATGTTTTTGATAATAGCGGATTTGACGA
TTTCATAAAATTTTCTATTTGAGAATATTTTCGGTTTTTAGAACGTCTAT
TGGTTTATTTCTACAGTTTTGTAGAGTTTATGTACATTGATGTTTTTAT

protein sequence of SMED30032411-orf-1

>SMED30032411-orf-1 ID=SMED30032411-orf-1|Name=SMED30032411-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=803bp
MSDSITVKLKINSAHKLIKRSLLKNPDPFAQIIYNNHSFCTNAQEKTTNP
AWNKSFHIELHSIKDKLEIIIWNQRVYDQKKSSAFLGSAVLTADFIDRGE
RRLQLARNNAFPSKPVRGFVKVEMIKIDSHSIPTNLSMPQIIHINGSSRG
LTAENSQQSLRYSGNQSSQRNSKEHSFSNDENNSRPGPSQISHGDLLLPI
NFERKISDGGKVYFVDNNSGAIHWYHPAVPKCLRKIGDFSCLGLLPPGWD
SLQSDSGRVYFIDHISRTTQYSDPRLLRALEAPDGYATVIRKLQNSGAVI
KNQSGYYTKIERSRSDRRRSNGTKLESSQTAVTFNLLESSAEINNQSTNH
TETIQTNRRSQSSGQIPSFNNQAILDMELSMSNISEDKNIDCNNELAPLP
LQRRRISRPTSNYILMNKNNIEIKLRDLRSKLKELMQKKLCIIETSRDNI
LEESFRNISRLKPPDLRKRLVLKFTDEEGLDYGGVAREWLYLLSETLFSC
RHGLFETNGDNDGNLLRVSPLCIVNEDHLHYFHFIGRVIGMAIFHGYYID
ADFCKIFYKSILNKQPQLEDFEELDKSIYNSLVWMRDNNIDDADLNLTFS
IEQEVFGQLELIDLCENGRFIPVTDNSKSQYIERYVDYNLSEKCAEQYEQ
IKKGIYEVLPQQILDNLNEDEFEVLLTGISEIKIEDWQKNTRLKHCLENE
PLIKWFWEVVTSYTEQQRTKLLQFVTGSSRIPLSGFEGLRGSYGSDEPKL
FTIKVIRNANPLSLPKAHTCFNRLDLPPYPSYKCLHDKLTCAIEETCGFA
VM*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0016567	protein ubiquitination

Vocabulary: **molecular function**
Term	Definition
GO:0061630	ubiquitin protein ligase activity
GO:0005515	protein binding
GO:0004842	ubiquitin-protein transferase activity
GO:0016740	transferase activity
GO:0016874	ligase activity

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000101	muscle cell
PLANA:0000142	posterior region of the whole animal

Vocabulary: **INTERPRO**
Term	Definition
IPR024928	E3_ub_ligase_SMURF1
IPR035892	C2_domain_sf
IPR036020	WW_dom_sf
IPR035983	Hect_E3_ubiquitin_ligase
IPR001202	WW_dom
IPR000008	C2_dom
IPR000569	HECT_dom

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 418..438
None	No IPR available	GENE3D	G3DSA:3.30.2410.10		coord: 679..802 e-value: 2.0E-41 score: 142.6
None	No IPR available	GENE3D	G3DSA:3.30.2160.10		coord: 565..643 e-value: 3.0E-87 score: 294.4
None	No IPR available	GENE3D	G3DSA:2.20.70.10		coord: 239..276 e-value: 2.2E-12 score: 48.7
None	No IPR available	GENE3D	G3DSA:3.90.1750.10		coord: 404..676 e-value: 3.0E-87 score: 294.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..190
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 345..367
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..173
None	No IPR available	PANTHER	PTHR11254:SF395	E3 UBIQUITIN-PROTEIN LIGASE SMURF1	coord: 4..136 coord: 145..801
None	No IPR available	PANTHER	PTHR11254	HECT DOMAIN UBIQUITIN-PROTEIN LIGASE	coord: 4..136 coord: 145..801
None	No IPR available	SUPERFAMILY	SSF49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 6..112
IPR000569	HECT domain	SMART	SM00119	hect_3	coord: 460..800 e-value: 5.0E-114 score: 394.8
IPR000569	HECT domain	PFAM	PF00632	HECT	coord: 491..801 e-value: 7.8E-89 score: 298.2
IPR000569	HECT domain	PROSITE	PS50237	HECT	coord: 462..802 score: 90.638
IPR000569	HECT domain	CDD	cd00078	HECTc	coord: 446..799 e-value: 2.64576E-129 score: 388.076
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 6..105 e-value: 1.5E-7 score: 41.1
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 7..100 e-value: 1.2E-10 score: 41.6
IPR000008	C2 domain	PROSITE	PS50004	C2	coord: 1..90 score: 10.175
IPR001202	WW domain	SMART	SM00456	ww_5	coord: 244..276 e-value: 3.6E-8 score: 43.1 coord: 197..229 e-value: 1.3 score: 15.5
IPR001202	WW domain	PFAM	PF00397	WW	coord: 245..274 e-value: 2.8E-6 score: 27.3
IPR001202	WW domain	PROSITE	PS01159	WW_DOMAIN_1	coord: 249..274
IPR001202	WW domain	PROSITE	PS50020	WW_DOMAIN_2	coord: 243..276 score: 15.072
IPR001202	WW domain	CDD	cd00201	WW	coord: 203..227 e-value: 0.00513206 score: 33.2702
IPR001202	WW domain	CDD	cd00201	WW	coord: 246..276 e-value: 5.47795E-8 score: 47.1374
IPR024928	E3 ubiquitin-protein ligase, SMURF1 type	PIRSF	PIRSF001569	E3_ub_ligase_SMURF1	coord: 405..801 e-value: 2.2E-132 score: 440.7 coord: 281..405 e-value: 190.0 score: -2.3 coord: 134..293 e-value: 1.1E-14 score: 51.4 coord: 1..110 e-value: 2.6E-9 score: 33.6
IPR035892	C2 domain superfamily	GENE3D	G3DSA:2.60.40.150		coord: 2..126 e-value: 7.4E-16 score: 60.3
IPR036020	WW domain superfamily	SUPERFAMILY	SSF51045	WW domain	coord: 243..278
IPR036020	WW domain superfamily	SUPERFAMILY	SSF51045	WW domain	coord: 194..231
IPR035983	HECT, E3 ligase catalytic domain	SUPERFAMILY	SSF56204	Hect, E3 ligase catalytic domain	coord: 431..795