SMED30032382
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032382 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Alignments
SMED30032382 aligns in the following genomic locations:
Homology
BLAST of SMED30032382 vs. Planmine SMEST
Match: SMESG000062659.1 (SMESG000062659.1) HSP 1 Score: 454.521 bits (1168), Expect = 1.113e-163 Identity = 236/241 (97.93%), Postives = 238/241 (98.76%), Query Frame = 1 Query: 58 MQIKSFLSIFLLHLFTSAFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLTVNHRQVPAIKQPRKTNSKVENWLSFEILNILREIRTXXXXXXXXXXXXXXXSIGIFVVWKRRSRLTKQRQYFKTLYNGSLMIPGTV 780 MQIKSFLSIFLLHLFTSAFATWSPDMKLHSANCQTATIETSCPNNT+DYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLTVNHRQVPAIKQPR+TNSKVENWLSFEILNILREIRTLLIVLFVMIILMIVFSIGIFVVWKRRSR TKQRQY K LYNGSLMIPGTV Sbjct: 1 MQIKSFLSIFLLHLFTSAFATWSPDMKLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLTVNHRQVPAIKQPRETNSKVENWLSFEILNILREIRTLLIVLFVMIILMIVFSIGIFVVWKRRSRSTKQRQYSKALYNGSLMIPGTV 241
BLAST of SMED30032382 vs. Planmine SMEST
Match: SMESG000035957.1 (SMESG000035957.1) HSP 1 Score: 122.094 bits (305), Expect = 3.304e-33 Identity = 85/238 (35.71%), Postives = 137/238 (57.56%), Query Frame = 1 Query: 82 IFLLHLFTSAFAT---WSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPA---SSIRLTVNHRQVPAIKQPRKTNSKVENWLSFEILNILREIRTXXXXXXXXXXXXXXXSIGIFVV---WKRRSRLTKQRQYFKTLYNGSLMI 768 I +L +F S T W+P+ KL ANC IET+CPN +++I L+T +N QE+ L MR C+ K++ T S+ CK LY+V V L+ +NL +C KDC K+ +SS WYLG+ CD+ SN+ Q +++K ++N C A +S L + R ++ +N+ + N L L LRE++ L+I +F +I+ I+ + IF++ +KRRS Q++ +K Y+ +I Sbjct: 7 ILMLAMFGSLSPTPLEWNPNTKLIKANCHELLIETTCPNVKTHDVMEIDLLTNINMQEVVLSVTMRKCKTPNQKMLTT-LSITCKRLYKVNVGLISENLISCIKDCPKSFQSSSWYLGKSKCDQELSNEMQNDQFKVSENECSATTKTSEILPSSRRSTTRVRARANSNADILNVL----LIYLREVKYLIIKIFTAVIIFIIL-VSIFIIKSCFKRRSHSQIQQRSWKDYYDMKSLI 238
BLAST of SMED30032382 vs. Planmine SMEST
Match: SMESG000058883.1 (SMESG000058883.1) HSP 1 Score: 78.1814 bits (191), Expect = 5.059e-16 Identity = 43/138 (31.16%), Postives = 71/138 (51.45%), Query Frame = 1 Query: 100 FTSAFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASS 510 T+ + WSP++K+ A C T+ CP ++I+LI N+E R C+L+ S+ ++ F++KC +Y V++ P + C+ CS L S + LG C + +D +Y+ YKT N+CP SS Sbjct: 328 LTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEEFS----TRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSS 461 HSP 2 Score: 67.781 bits (164), Expect = 1.550e-12 Identity = 48/182 (26.37%), Postives = 88/182 (48.35%), Query Frame = 1 Query: 82 IFLLHLF--------TSAFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLTVNH------RQVPAIKQPRKTNSKVEN 585 I+L++LF S W+PD++L + C T+ CP + ++ L + + ++ + CR+++S+ + T FS++CK LY+V ++L P + +C + CS S +W LG C E + D + + +N C SSI+ +++ R P PR N+ +N Sbjct: 28 IYLINLFIFYICCNWVSLVPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQTLQ--CRINQSQPLTTTFSIQCKNLYRVNISLSP--IFSCTEYCSDIHTSRIWLLGGNTCGEI-ALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNPRLVNAYCKN 204 HSP 3 Score: 57.3806 bits (137), Expect = 4.109e-9 Identity = 39/130 (30.00%), Postives = 74/130 (56.92%), Query Frame = 1 Query: 136 KLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQY-EKYKTTKNICPASSIRLT 522 +L +A C+ T+E SCP + +D I I ++NQ+ LE++ + C+ + +KL+Q F + CK Y V+V+L+ +L N +K + +S+ W LG++ C ++ +++K +N C S++ T Sbjct: 196 RLVNAYCKNVTVEASCPQSPEDQY--IEFILSLSNQK-HLESISKLCQSNATKLVQVTFKISCKNFYYVEVSLLSSSLKN-SKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCT 321 HSP 4 Score: 50.447 bits (119), Expect = 7.459e-7 Identity = 36/141 (25.53%), Postives = 63/141 (44.68%), Query Frame = 1 Query: 103 TSAFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLT 522 +S+ W +K+ C T++ SCP+ D ++ L N+Q ++ +V C S+ + HF ++C+ LYQ NL+ N + K ++++ S W G C E + N Q KY+ C R + Sbjct: 471 SSSHKIWESQLKIVQDFCSNVTVKASCPDLKD--RINFLLFEKGNSQPIKGRSV--PCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRCS 607
BLAST of SMED30032382 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1) HSP 1 Score: 72.4034 bits (176), Expect = 4.004e-14 Identity = 46/139 (33.09%), Postives = 70/139 (50.36%), Query Frame = 1 Query: 121 WSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQ--SNDYQYEKYKTTKNICPASSIRLTVN 528 W P++ L SANC IE +CP + ++ SL F Q +Q + C S +K + + C LYQV ++L P + NC + C + S +W G++ C Q SN+ KY T KNICPAS+ + + + Sbjct: 21 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFP-TQNVQSVKTYK-CPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDI-CQNKQFLSNNGSLNKYMTPKNICPASTQKCSAD 156 HSP 2 Score: 71.633 bits (174), Expect = 6.919e-14 Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 1 Query: 136 KLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIR 516 KL A+C+ A + CP ++ ++ I + N +M+ C+ + S +I FS+ CK LY + ++ P N S C+ S +W LG C E ND QYE+YK+ +N C SS R Sbjct: 177 KLTDADCENAIFQMECP--LFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRR 301 HSP 3 Score: 59.3066 bits (142), Expect = 9.318e-10 Identity = 36/132 (27.27%), Postives = 65/132 (49.24%), Query Frame = 1 Query: 109 AFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSN-CNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICP 501 +F WSP++KL +A C T++ +CP L+ SL+ + ++ + V + C ++ F +C+ +Y V + + P S C++ CSK S +W LG C + + + KY+ +N CP Sbjct: 307 SFTQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLR-MGDRSIS-ATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHCP 436
BLAST of SMED30032382 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1) HSP 1 Score: 72.4034 bits (176), Expect = 4.172e-14 Identity = 46/139 (33.09%), Postives = 70/139 (50.36%), Query Frame = 1 Query: 121 WSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQ--SNDYQYEKYKTTKNICPASSIRLTVN 528 W P++ L SANC IE +CP + ++ SL F Q +Q + C S +K + + C LYQV ++L P + NC + C + S +W G++ C Q SN+ KY T KNICPAS+ + + + Sbjct: 24 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFP-TQNVQSVKTYK-CPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDI-CQNKQFLSNNGSLNKYMTPKNICPASTQKCSAD 159 HSP 2 Score: 71.633 bits (174), Expect = 6.946e-14 Identity = 41/127 (32.28%), Postives = 63/127 (49.61%), Query Frame = 1 Query: 136 KLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIR 516 KL A+C+ A + CP ++ ++ I + N +M+ C+ + S +I FS+ CK LY + ++ P N S C+ S +W LG C E ND QYE+YK+ +N C SS R Sbjct: 180 KLTDADCENAIFQMECP--LFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRR 304 HSP 3 Score: 59.3066 bits (142), Expect = 1.024e-9 Identity = 36/132 (27.27%), Postives = 65/132 (49.24%), Query Frame = 1 Query: 109 AFATWSPDMKLHSANCQTATIETSCPNNTDDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSN-CNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICP 501 +F WSP++KL +A C T++ +CP L+ SL+ + ++ + V + C ++ F +C+ +Y V + + P S C++ CSK S +W LG C + + + KY+ +N CP Sbjct: 310 SFTQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLR-MGDRSIS-ATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHCP 439 The following BLAST results are available for this feature:
BLAST of SMED30032382 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032382 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032382 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032382 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032382 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032382 ID=SMED30032382|Name=SMED30032382|organism=Schmidtea mediterranea sexual|type=transcript|length=869bpback to top protein sequence of SMED30032382-orf-1 >SMED30032382-orf-1 ID=SMED30032382-orf-1|Name=SMED30032382-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=242bp MQIKSFLSIFLLHLFTSAFATWSPDMKLHSANCQTATIETSCPNNTDDYMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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