PIGA protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032377 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of PIGA protein vs. TrEMBL
Match: A0A1I8HA02 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 93.2041 bits (230), Expect = 4.166e-20 Identity = 38/76 (50.00%), Postives = 61/76 (80.26%), Query Frame = 3 Query: 291 KQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQ 518 +++RFS+VA+++LR ++ D+HP+GRGG+F GVLY ++++K ++DH+K WI+F GF L S IIILAE++R+Q Sbjct: 43 QENRFSAVAIEMLREQEDRDLHPYGRGGRFEGVLY-SRRVKVQVDHAKFLAWIVFAGFILLTSGAIIILAEMERRQ 117
BLAST of PIGA protein vs. TrEMBL
Match: A0A1I8IHX0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 93.5893 bits (231), Expect = 3.756e-18 Identity = 38/76 (50.00%), Postives = 61/76 (80.26%), Query Frame = 3 Query: 291 KQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQ 518 +++RFS+VA+++LR ++ D+HP+GRGG+F GVLY ++++K ++DH+K WI+F GF L S IIILAE++R+Q Sbjct: 43 QENRFSAVAIEMLREQEDRDLHPYGRGGRFEGVLY-SRRVKVQVDHAKFLAWIVFAGFILLTSGAIIILAEMERRQ 117
BLAST of PIGA protein vs. TrEMBL
Match: A0A1I8HJ73 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 70.4774 bits (171), Expect = 1.829e-11 Identity = 28/57 (49.12%), Postives = 44/57 (77.19%), Query Frame = 3 Query: 282 NKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWIL 452 NK RFS+VA+++LR ++ D+HPFGRGG+F GVLY ++++K ++DH+K WI+ Sbjct: 68 NKELDKRFSAVAMEMLREQEDRDLHPFGRGGRFEGVLY-SRRVKVQVDHTKFLAWIV 123
BLAST of PIGA protein vs. TrEMBL
Match: A0A1I8HLH4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 3.911e-8 Identity = 27/52 (51.92%), Postives = 38/52 (73.08%), Query Frame = 3 Query: 282 NKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQK---IKNRIDH 428 NK RFS+VA+++LR ++ D+HPFGRGG+F GVLY +K +KNR +H Sbjct: 130 NKELDKRFSAVAMEMLREQEDRDLHPFGRGGRFEGVLYSRRKWNAVKNRNNH 181
BLAST of PIGA protein vs. TrEMBL
Match: A0A183WGT4 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS11334 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 6.011e-8 Identity = 27/79 (34.18%), Postives = 48/79 (60.76%), Query Frame = 3 Query: 270 STNP--NKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILA 500 +T P + +Q R SSV L++L + DIHP+GRGG + G+LY + + + ++D + FVW +F+ + S I+ ++ Sbjct: 65 TTRPVLTEEQQMRLSSVTLEMLMEREEIDIHPYGRGGIYEGILYKS-RAEEQMDFTAAFVWFIFVSSVIMMSGSIVFVS 142
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000081746.1 (SMESG000081746.1) HSP 1 Score: 251.906 bits (642), Expect = 3.526e-86 Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 3 Query: 177 MSSKNENCVNGNCETLVEMIENDTLNFMLSMSTNPNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQTKSFGSIT 542 MSSKNENCVNGNCETLVEMIENDTLNFMLSMSTNPNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQTKSFGSIT Sbjct: 1 MSSKNENCVNGNCETLVEMIENDTLNFMLSMSTNPNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQTKSFGSIT 122
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000079358.1 (SMESG000079358.1) HSP 1 Score: 74.3294 bits (181), Expect = 1.301e-16 Identity = 43/103 (41.75%), Postives = 64/103 (62.14%), Query Frame = 3 Query: 213 CETLVEMIENDTLNFMLSMSTN---PNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDR 512 + L++ +E +T F+ S + + N K RFS+VA+D+LR ++ DIHP+GRGG+F GVLY +KI+ R++ +K W+ IG L S II LAE R Sbjct: 31 SDKLIQSVEENTNAFISSYNKDKLYSNVPKVPRFSTVAMDILRSTEDHDIHPYGRGGRFEGVLYTGRKIRIRLESNKWLAWLTLIGLLLMLSLTIIFLAEQHR 133
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000048041.1 (SMESG000048041.1) HSP 1 Score: 62.3882 bits (150), Expect = 2.792e-12 Identity = 31/75 (41.33%), Postives = 42/75 (56.00%), Query Frame = 3 Query: 297 HRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQT 521 RFS +A+ +L+ D D HP+GRG G + K+ D S I VW +F+GF L +IIILAE+ R Q Sbjct: 53 RRFSDIAITMLKDKDETDEHPYGRGKTVDGCFNRSSSGKDS-DQSMIIVWFIFVGFLLGSCVMIIILAELYRSQA 126
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000047330.1 (SMESG000047330.1) HSP 1 Score: 60.8474 bits (146), Expect = 1.021e-11 Identity = 23/59 (38.98%), Postives = 40/59 (67.80%), Query Frame = 3 Query: 342 NEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDRKQ 518 + DIHP+G ++ +++G +++K ++DH+K WI F+ F L SA II+LAE +R + Sbjct: 47 DRDIHPYGETRCYSDIIFGQREVKVKLDHTKFLAWISFVVFLLIISATIIVLAEFERTE 105 The following BLAST results are available for this feature:
BLAST of PIGA protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of PIGA protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of PIGA protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of PIGA protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of PIGA protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of PIGA protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of PIGA protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032377 ID=SMED30032377|Name=PIGA protein|organism=Schmidtea mediterranea sexual|type=transcript|length=719bpback to top protein sequence of SMED30032377-orf-1 >SMED30032377-orf-1 ID=SMED30032377-orf-1|Name=SMED30032377-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp MSSKNENCVNGNCETLVEMIENDTLNFMLSMSTNPNKSKQHRFSSVALDLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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