SMED30032366
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032366 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 34
Alignments
SMED30032366 aligns in the following genomic locations:
Homology
BLAST of SMED30032366 vs. UniProt/SwissProt
Match: sp|Q84177|PRO_ADEP3 (Protease OS=Porcine adenovirus A serotype 3 OX=35265 GN=L3 PE=3 SV=1) HSP 1 Score: 49.6766 bits (117), Expect = 5.531e-7 Identity = 35/113 (30.97%), Postives = 50/113 (44.25%), Query Frame = 1 Query: 88 EFLGVFPLDKIPYLRNNQ--AIIVNTQSSHLGGEHWLAIYMKPSK--IFVFDPMGF------------YYPALMVSKLQTMGK---PIVYNKTMYQKPWTLTCGQHCLAWLLS 369 FLG F +L+ +Q IVNT + H GG HWLA+ +P+ + FDP GF Y L S +Q+ +V + Q P + CG CL +L + Sbjct: 19 RFLGTFDKSFPGFLQESQRCCAIVNTAARHTGGRHWLAVAWEPASRTFYFFDPFGFSDRELAQVYDFEYQRLLRKSAIQSTPDRCLTLVKSTQSVQGPHSAACGLFCLLFLAA 131
BLAST of SMED30032366 vs. TrEMBL
Match: A0A3C1S1Q5 (Uncharacterized protein OS=Tenacibaculum sp. OX=1906242 GN=DDE71_02150 PE=4 SV=1) HSP 1 Score: 67.3958 bits (163), Expect = 2.136e-11 Identity = 34/111 (30.63%), Postives = 59/111 (53.15%), Query Frame = 1 Query: 40 MDSVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQA--IIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMG--FYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAW 360 MDS ++ + KQ +FLGVF +D++ + N+ +I NT +S G+HW+A+ + ++IF FD + F Y ++ K +++N Q + CG HCL + Sbjct: 1 MDSNEINKFAKQFGLKKFLGVFAVDELSLIPKNRLGLVIFNTDTSQSVGQHWVALCITKTRIFYFDSLNIKFQYSTHFTDFMKYTKKELIWNTIQIQSNISEKCGIHCLVF 111
BLAST of SMED30032366 vs. TrEMBL
Match: A0A443S1W5 (Uncharacterized protein (Fragment) OS=Leptotrombidium deliense OX=299467 GN=B4U80_06898 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 1.394e-9 Identity = 37/96 (38.54%), Postives = 50/96 (52.08%), Query Frame = 1 Query: 88 EFLGVFPLDKIPYL-RNNQAIIVNTQSSHLGGEHWLAIYM-KPSKIFVFDPMGF--YYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWL 363 EF+G FP D IP R + IVNT SS+ GEHW+AIY+ K +K FD G + L + + YN Q P + TCG+ CL ++ Sbjct: 3 EFVGTFPRDNIPTTTRRPASFIVNTDSSNEPGEHWVAIYLTKNNKAEYFDSFGLPPLHRDLTEFIHEHAKNGVKYNNICIQHPLSTTCGKFCLKYV 98
BLAST of SMED30032366 vs. TrEMBL
Match: A0A4Y2MES2 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_200179_1 PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 2.602e-9 Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 1 Query: 91 FLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMG-----FYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWLLSK 372 F GVFP D IP L AIIVNT S G+HW+A++M+ K+ FD G F + S YN +Q +L CG + + ++L + Sbjct: 20 FQGVFPADNIPLLTGKAAIIVNTDVSGGHGKHWVALFMESDKLDFFDSFGKSPDEFLNGSFHTSCFAEKFPNTTYNPIKFQSRESLVCGYYSIYFILQR 118
BLAST of SMED30032366 vs. TrEMBL
Match: A0A1A8V644 (Uncharacterized protein (Fragment) OS=Nothobranchius furzeri OX=105023 GN=LOTGIDRAFT_175928 PE=4 SV=1) HSP 1 Score: 60.4622 bits (145), Expect = 5.283e-9 Identity = 39/121 (32.23%), Postives = 60/121 (49.59%), Query Frame = 1 Query: 40 MDSVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQAI----IVNTQSSHLGGEHWLAIYMKPSKIFV-FDPMGF-----YYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWLLSK 372 MD+ +++ I +LG F GVF DK+ + + +VNT +HL GEHWLA+ ++ + FD GF YYP +++ L+ I Y Q + CG HC+ +L K Sbjct: 1 MDNHELHGILSSLLGDLFSGVFASDKLSTIPKKIQLLAYFVVNTHPAHLPGEHWLALPVEQDGLGTFFDSYGFSPEFEYYPKTIMNFLKERCSEIHYQDDHLQSLTSDRCGHHCVLFLCHK 121
BLAST of SMED30032366 vs. TrEMBL
Match: A0A2B4RXN3 (Pulmonary surfactant-associated protein D OS=Stylophora pistillata OX=50429 GN=Sftpd PE=4 SV=1) HSP 1 Score: 62.7734 bits (151), Expect = 1.065e-8 Identity = 30/91 (32.97%), Postives = 45/91 (49.45%), Query Frame = 1 Query: 91 FLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWL 363 F GV D++ + + ++N SH G HW+A++ I FD G + P +VS T G YN T YQ ++ CG +CL +L Sbjct: 952 FGGVISRDQVKDCKPKRCYVINLNESHESGSHWVAMFFGSDLILYFDSFGLHPPQEIVSLSNTEGINYAYNNTHYQALLSVLCGYYCLYFL 1042
BLAST of SMED30032366 vs. Planmine SMEST
Match: SMESG000006220.1 (SMESG000006220.1) HSP 1 Score: 220.32 bits (560), Expect = 2.156e-67 Identity = 102/108 (94.44%), Postives = 103/108 (95.37%), Query Frame = 1 Query: 52 QVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWLLSKR 375 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLA + R Sbjct: 353 NVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLALVYFNR 460
BLAST of SMED30032366 vs. Planmine SMEST
Match: SMESG000050353.1 (SMESG000050353.1) HSP 1 Score: 216.083 bits (549), Expect = 6.085e-66 Identity = 99/105 (94.29%), Postives = 101/105 (96.19%), Query Frame = 1 Query: 46 SVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAW 360 +V VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKP KIFVFDPMGFYYPAL+VSKLQ MGKPIVYNKTMYQK WTLTCGQHCLAW Sbjct: 270 TVLVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPCKIFVFDPMGFYYPALVVSKLQAMGKPIVYNKTMYQKQWTLTCGQHCLAW 374
BLAST of SMED30032366 vs. Planmine SMEST
Match: SMESG000043200.1 (SMESG000043200.1) HSP 1 Score: 187.963 bits (476), Expect = 1.842e-59 Identity = 85/112 (75.89%), Postives = 95/112 (84.82%), Query Frame = 1 Query: 46 SVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMGKPIVYNKTMYQKPWTLTCGQHCLAWLLSKRFD 381 S+QV R+G +LG+EF+GV+ LDKIPYLRNNQAIIV TQS HL GEHW+AIYMKP KI VFDP+GFY PALMVSKLQTM KPI+YNKTMYQ P T T GQHCL WL+ KRFD Sbjct: 272 SIQVNRLGNLLLGNEFVGVYRLDKIPYLRNNQAIIVITQSFHLSGEHWIAIYMKPDKIHVFDPLGFYSPALMVSKLQTMNKPIMYNKTMYQNPMTRTYGQHCLTWLMYKRFD 383
BLAST of SMED30032366 vs. Planmine SMEST
Match: SMESG000022236.1 (SMESG000022236.1) HSP 1 Score: 168.703 bits (426), Expect = 8.653e-51 Identity = 75/76 (98.68%), Postives = 75/76 (98.68%), Query Frame = 1 Query: 55 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTM 282 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYM PSKIFVFDPMGFYYPALMVSKLQTM Sbjct: 59 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMNPSKIFVFDPMGFYYPALMVSKLQTM 134
BLAST of SMED30032366 vs. Planmine SMEST
Match: SMESG000022236.1 (SMESG000022236.1) HSP 1 Score: 169.474 bits (428), Expect = 2.291e-50 Identity = 76/79 (96.20%), Postives = 77/79 (97.47%), Query Frame = 1 Query: 46 SVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTM 282 +V VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYM PSKIFVFDPMGFYYPALMVSKLQTM Sbjct: 85 TVLVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMNPSKIFVFDPMGFYYPALMVSKLQTM 163 The following BLAST results are available for this feature:
BLAST of SMED30032366 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032366 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30032366 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30032366 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032366 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032366 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032366 ID=SMED30032366|Name=SMED30032366|organism=Schmidtea mediterranea sexual|type=transcript|length=385bpback to top protein sequence of SMED30032366-orf-1 >SMED30032366-orf-1 ID=SMED30032366-orf-1|Name=SMED30032366-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp MDSVQVYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWback to top Annotated Terms
The following terms have been associated with this transcript:
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