VASt domain-containing protein

Overview
NameVASt domain-containing protein
Smed IDSMED30032364
Length (bp)2521
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of VASt domain-containing protein (SMED30032364) t-SNE clustered cells

Violin plots show distribution of expression levels for VASt domain-containing protein (SMED30032364) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of VASt domain-containing protein (SMED30032364) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for VASt domain-containing protein (SMED30032364) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032364

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30032364h1SMcG0012306 PL08008B2B02smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
epidermisSMED30032364h1SMcG0012306 dd_Smed_v4_9355_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30032364h1SMcG0012306 dd_Smed_v4_9355_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30032364h1SMcG0012306 dd_Smed_v4_9355_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of VASt domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 206.838 bits (525), Expect = 5.153e-56
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   51 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 509          
BLAST of VASt domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 206.838 bits (525), Expect = 5.205e-56
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   51 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 509          
BLAST of VASt domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 206.838 bits (525), Expect = 6.553e-56
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   55 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKK--EENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 513          
BLAST of VASt domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 206.838 bits (525), Expect = 1.064e-55
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 549          
BLAST of VASt domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 206.838 bits (525), Expect = 1.085e-55
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 549          
BLAST of VASt domain-containing protein vs. Ensembl Celegans
Match: ZC328.3 (pep chromosome:WBcel235:I:6409050:6417010:-1 gene:WBGene00022593.1 transcript:ZC328.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC328.3)

HSP 1 Score: 143.28 bits (360), Expect = 2.034e-35
Identity = 127/461 (27.55%), Postives = 196/461 (42.52%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSD--ASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSG-KYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEAD-----IYISEECN------------INQKNNLSENEIMKN-------SEVLLRTEAPSFVVGDNSDSD-TDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLA 1724
            +Y  + + FKKIF D      NDQ L  YSCA  +    IL QGR+++++  +CFY+ I+ +E  +++PM  IS V K K   + PN+IQI   +  KYFF S              +N+ K+ + L    +K    D      +     +Y SE+ N             + KN  +EN  M N       S +  RTEA +  + +N+D D T QS  SS+  H ++   H                  +E    ED  +        +P                    C   EHF RL +D+E  + V++ +  LFT++ F     +  KT + + A W    +  G   RT  YT+++   + PK     EKQI+     P    ++    +N G P SD F    RYCI        ++KV   V+Y KS +   K+ IE     GL +++  L+
Sbjct:   68 SYHERNLQFKKIFVDKGLIEENDQFLASYSCAYQRE---ILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTKEKYFFAS-------------FINRDKSYQVLTTAHQKVAGEDEKAMTREEVWDMVYNSEDKNSQNQTPPDGSTPASTKNTSTEN--MTNLSSRSFNSSISPRTEAST--LKNNNDKDNTSQSSTSSDF-HDEDSATHF-----------------SEQFEQEDEIIEE------VP--------------------CPCSEHFGRLIMDKEFKVSVEKFYELLFTDNEFLTMLNQKTKTAEYVAATWVR--DHQGDNTRTCTYTVSLAHAMAPKAIIVNEKQILTHYPNPKQGIMMQKETQNSGVPYSDNFTVNCRYCISRTGPTSCRIKVHGGVMYKKSTWAVVKTFIEKGTHQGLDEHYQLLS 462          
BLAST of VASt domain-containing protein vs. Ensembl Celegans
Match: ZC328.3 (pep chromosome:WBcel235:I:6409050:6412249:-1 gene:WBGene00022593.1 transcript:ZC328.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC328.3)

HSP 1 Score: 90.5077 bits (223), Expect = 4.589e-19
Identity = 51/169 (30.18%), Postives = 80/169 (47.34%), Query Frame = 3
Query: 1218 CNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLA 1724
            C   EHF RL +D+E  + V++ +  LFT++ F     +  KT + + A W    +  G   RT  YT+++   + PK     EKQI+     P    ++    +N G P SD F    RYCI        ++KV   V+Y KS +   K+ IE     GL +++  L+
Sbjct:   65 CPCSEHFGRLIMDKEFKVSVEKFYELLFTDNEFLTMLNQKTKTAEYVAATWVR--DHQGDNTRTCTYTVSLAHAMAPKAIIVNEKQILTHYPNPKQGIMMQKETQNSGVPYSDNFTVNCRYCISRTGPTSCRIKVHGGVMYKKSTWAVVKTFIEKGTHQGLDEHYQLLS 231          
BLAST of VASt domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112638)

HSP 1 Score: 115.931 bits (289), Expect = 5.756e-26
Identity = 60/157 (38.22%), Postives = 98/157 (62.42%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVL-----QTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNIN 881
            NY+++   FKK+F D   ++++L+ DYSCAL +    IL+QGRLYV++ ++CF++ I S+E  + +    ++ ++KEKT  VIPNAI I++   KYFF +F SR+KSF++L      TL+NK  + + +        +H CYG E  +   +E  I+
Sbjct:  405 NYKSRAEDFKKLFKDV-PNDERLIVDYSCALQRD---ILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQH-----VHTCYGDELGLTTDDEDYID 552          

HSP 2 Score: 102.064 bits (253), Expect = 1.027e-21
Identity = 60/188 (31.91%), Postives = 92/188 (48.94%), Query Frame = 3
Query: 1224 THEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE-----AKGLGQHLRQ 1772
            T  H  R  V   + ++VD LF+ LF+ S F   F   RK+ D++   W +  NE G + RT+  T+ + A +GPK S   E Q     S PG  Y +D    N G P ++ F  +  +C+       + L + +Q+ Y KSI+   K  IE    +GL D+F    ++L+ E     AKG G+  R+
Sbjct:  808 TSTHEGRQIVHTILPINVDTLFNLLFSKSKFITDFHAMRKSTDLVLGEWTK--NEEGLQVRTVNVTVQLAASVGPKTSKVTEYQTQRECSKPGELYSIDVNSVNAGIPYAESFSVLIHFCLARTVDDHTMLSIHTQIKYKKSIWGVVKGFIEKNTWAGLEDFFGAQLHALQSETCIPPAKGKGRRPRR 993          
BLAST of VASt domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0332409)

HSP 1 Score: 115.931 bits (289), Expect = 6.145e-26
Identity = 65/176 (36.93%), Postives = 105/176 (59.66%), Query Frame = 3
Query:  369 TISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVL-----QTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNIN 881
            T  L+   +KKS       NY+++   FKK+F D   ++++L+ DYSCAL +    IL+QGRLYV++ ++CF++ I S+E  + +    ++ ++KEKT  VIPNAI I++   KYFF +F SR+KSF++L      TL+NK  + + +        +H CYG E  +   +E  I+
Sbjct:  288 TSRLSERAKKKSWYNVIYPNYKSRAEDFKKLFKDV-PNDERLIVDYSCALQRD---ILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQH-----VHTCYGDELGLTTDDEDYID 454          

HSP 2 Score: 102.064 bits (253), Expect = 1.145e-21
Identity = 60/188 (31.91%), Postives = 92/188 (48.94%), Query Frame = 3
Query: 1224 THEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE-----AKGLGQHLRQ 1772
            T  H  R  V   + ++VD LF+ LF+ S F   F   RK+ D++   W +  NE G + RT+  T+ + A +GPK S   E Q     S PG  Y +D    N G P ++ F  +  +C+       + L + +Q+ Y KSI+   K  IE    +GL D+F    ++L+ E     AKG G+  R+
Sbjct:  710 TSTHEGRQIVHTILPINVDTLFNLLFSKSKFITDFHAMRKSTDLVLGEWTK--NEEGLQVRTVNVTVQLAASVGPKTSKVTEYQTQRECSKPGELYSIDVNSVNAGIPYAESFSVLIHFCLARTVDDHTMLSIHTQIKYKKSIWGVVKGFIEKNTWAGLEDFFGAQLHALQSETCIPPAKGKGRRPRR 895          
BLAST of VASt domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112641)

HSP 1 Score: 115.546 bits (288), Expect = 6.474e-26
Identity = 60/157 (38.22%), Postives = 98/157 (62.42%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVL-----QTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNIN 881
            NY+++   FKK+F D   ++++L+ DYSCAL +    IL+QGRLYV++ ++CF++ I S+E  + +    ++ ++KEKT  VIPNAI I++   KYFF +F SR+KSF++L      TL+NK  + + +        +H CYG E  +   +E  I+
Sbjct:  331 NYKSRAEDFKKLFKDV-PNDERLIVDYSCALQRD---ILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQH-----VHTCYGDELGLTTDDEDYID 478          

HSP 2 Score: 102.449 bits (254), Expect = 8.987e-22
Identity = 60/188 (31.91%), Postives = 92/188 (48.94%), Query Frame = 3
Query: 1224 THEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE-----AKGLGQHLRQ 1772
            T  H  R  V   + ++VD LF+ LF+ S F   F   RK+ D++   W +  NE G + RT+  T+ + A +GPK S   E Q     S PG  Y +D    N G P ++ F  +  +C+       + L + +Q+ Y KSI+   K  IE    +GL D+F    ++L+ E     AKG G+  R+
Sbjct:  734 TSTHEGRQIVHTILPINVDTLFNLLFSKSKFITDFHAMRKSTDLVLGEWTK--NEEGLQVRTVNVTVQLAASVGPKTSKVTEYQTQRECSKPGELYSIDVNSVNAGIPYAESFSVLIHFCLARTVDDHTMLSIHTQIKYKKSIWGVVKGFIEKNTWAGLEDFFGAQLHALQSETCIPPAKGKGRRPRR 919          
BLAST of VASt domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112639)

HSP 1 Score: 115.546 bits (288), Expect = 6.711e-26
Identity = 60/157 (38.22%), Postives = 98/157 (62.42%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVL-----QTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNIN 881
            NY+++   FKK+F D   ++++L+ DYSCAL +    IL+QGRLYV++ ++CF++ I S+E  + +    ++ ++KEKT  VIPNAI I++   KYFF +F SR+KSF++L      TL+NK  + + +        +H CYG E  +   +E  I+
Sbjct:  331 NYKSRAEDFKKLFKDV-PNDERLIVDYSCALQRD---ILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQH-----VHTCYGDELGLTTDDEDYID 478          

HSP 2 Score: 102.064 bits (253), Expect = 9.331e-22
Identity = 60/188 (31.91%), Postives = 92/188 (48.94%), Query Frame = 3
Query: 1224 THEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE-----AKGLGQHLRQ 1772
            T  H  R  V   + ++VD LF+ LF+ S F   F   RK+ D++   W +  NE G + RT+  T+ + A +GPK S   E Q     S PG  Y +D    N G P ++ F  +  +C+       + L + +Q+ Y KSI+   K  IE    +GL D+F    ++L+ E     AKG G+  R+
Sbjct:  734 TSTHEGRQIVHTILPINVDTLFNLLFSKSKFITDFHAMRKSTDLVLGEWTK--NEEGLQVRTVNVTVQLAASVGPKTSKVTEYQTQRECSKPGELYSIDVNSVNAGIPYAESFSVLIHFCLARTVDDHTMLSIHTQIKYKKSIWGVVKGFIEKNTWAGLEDFFGAQLHALQSETCIPPAKGKGRRPRR 919          
BLAST of VASt domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0306333)

HSP 1 Score: 115.546 bits (288), Expect = 7.276e-26
Identity = 60/157 (38.22%), Postives = 98/157 (62.42%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVL-----QTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNIN 881
            NY+++   FKK+F D   ++++L+ DYSCAL +    IL+QGRLYV++ ++CF++ I S+E  + +    ++ ++KEKT  VIPNAI I++   KYFF +F SR+KSF++L      TL+NK  + + +        +H CYG E  +   +E  I+
Sbjct:  341 NYKSRAEDFKKLFKDV-PNDERLIVDYSCALQRD---ILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQH-----VHTCYGDELGLTTDDEDYID 488          

HSP 2 Score: 102.449 bits (254), Expect = 9.025e-22
Identity = 60/188 (31.91%), Postives = 92/188 (48.94%), Query Frame = 3
Query: 1224 THEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE-----AKGLGQHLRQ 1772
            T  H  R  V   + ++VD LF+ LF+ S F   F   RK+ D++   W +  NE G + RT+  T+ + A +GPK S   E Q     S PG  Y +D    N G P ++ F  +  +C+       + L + +Q+ Y KSI+   K  IE    +GL D+F    ++L+ E     AKG G+  R+
Sbjct:  744 TSTHEGRQIVHTILPINVDTLFNLLFSKSKFITDFHAMRKSTDLVLGEWTK--NEEGLQVRTVNVTVQLAASVGPKTSKVTEYQTQRECSKPGELYSIDVNSVNAGIPYAESFSVLIHFCLARTVDDHTMLSIHTQIKYKKSIWGVVKGFIEKNTWAGLEDFFGAQLHALQSETCIPPAKGKGRRPRR 929          
BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 192.586 bits (488), Expect = 3.553e-51
Identity = 167/642 (26.01%), Postives = 288/642 (44.86%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNH-PDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPS-TNESNSMYR-TSLLHNVAQICNTHEHFH----------RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXX----------XXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKA--NLENLDDEIKSNSFHF 2279
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++ +    M   E     E P+    DN  +D        N N  PD   D     I  ++ R  F + +  S  + D+ +N   +  S  + T   N  +    LL N      TH+             +L+++E     VD+L   LFT S F   F E R+  D++  PW     + G + R I YTI++   + PK ++  E Q +H  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L      L +   +      AT     +P+    P       S +    ++ ++    +    S      PD A   L + S+             L +   Y     LE       + Q   S +     +  +W  +++ QQ+    E+ +W+ +++SS+ L+D+MR  L+ ++    L + D + +    H+
Sbjct:  108 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFN---TMGYCE-----EIPA---EDNEAND--------NCNKTPDAKMD-----ISPQLHRKVFPNSSLNSTDSTDAPLNVAISGVSFRNMTYTQNGGFECVQLLVNSNDSSETHDEGEVQAFQEDLNGKLYINEVYKFSVDKLHDILFTESQFMREFLEQRRFSDVVYHPW--RKEDTGNQTREIMYTISLSNPLAPKTATVTETQTLHKASQVSECYIIDAEVIAHDVPYHDYFYTLNRYMLTRVARNKCRLRVSAELRYRKQPWGLVKGFIERNFWSGLDEYFRYLELELNKVQLVLSEQSPKSKALRTATVRRRKRPLTHMRPQHMDEALSPVTTPEDEKSVIQRIKHVAGSTQTRHMPDHASEGLALYSVSKLLLIISSVCLLLVVLGKYYLLWMLEYTAQSLASWQGLRSHESKLPQTQIEWAQLLESQQRYHESELEKWREIIKSSVLLLDEMRESLMKLQKGIGLRDYDSDSEERRSHY 719          
BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 191.43 bits (485), Expect = 9.039e-51
Identity = 130/464 (28.02%), Postives = 220/464 (47.41%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQS-------LYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAES-----------LSTEDSNVNSNFNSTSLPSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++   N +    E+       +       D+  D S         +S+LN  D     L+           FD P  E            L+  D+ ++    S  +P+ + S            +  +S  H+  ++    E  + +L+++E     VD+L   LFT S F   F E R+  D++  PW     + G + R I YTI++   + PK ++  E Q +H  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:   80 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDF--NTMGYCEEIPAEDNEANDNCNKTPDAKMDISPQLHRKVFPNSSLNSTDSTDAPLN-----------FDVPPVEDFTNCIPEDLLPLTLPDNKLSETGGSAHVPAPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFQEDLNGKLYINEVYKFSVDKLHDILFTESQFMREFLEQRRFSDVVYHPW--RKEDTGNQTREIMYTISLSNPLAPKTATVTETQTLHKASQVSECYIIDAEVIAHDVPYHDYFYTLNRYMLTRVARNKCRLRVSAELRYRKQPWGLVKGFIERNFWSGLDEYFRYLELEL 524          
BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 192.2 bits (487), Expect = 1.029e-50
Identity = 130/464 (28.02%), Postives = 220/464 (47.41%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQS-------LYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAES-----------LSTEDSNVNSNFNSTSLPSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++   N +    E+       +       D+  D S         +S+LN  D     L+           FD P  E            L+  D+ ++    S  +P+ + S            +  +S  H+  ++    E  + +L+++E     VD+L   LFT S F   F E R+  D++  PW     + G + R I YTI++   + PK ++  E Q +H  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:  142 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDF--NTMGYCEEIPAEDNEANDNCNKTPDAKMDISPQLHRKVFPNSSLNSTDSTDAPLN-----------FDVPPVEDFTNCIPEDLLPLTLPDNKLSETGGSAHVPAPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFQEDLNGKLYINEVYKFSVDKLHDILFTESQFMREFLEQRRFSDVVYHPW--RKEDTGNQTREIMYTISLSNPLAPKTATVTETQTLHKASQVSECYIIDAEVIAHDVPYHDYFYTLNRYMLTRVARNKCRLRVSAELRYRKQPWGLVKGFIERNFWSGLDEYFRYLELEL 586          
BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 191.43 bits (485), Expect = 1.519e-50
Identity = 130/464 (28.02%), Postives = 220/464 (47.41%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQS-------LYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAES-----------LSTEDSNVNSNFNSTSLPSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++   N +    E+       +       D+  D S         +S+LN  D     L+           FD P  E            L+  D+ ++    S  +P+ + S            +  +S  H+  ++    E  + +L+++E     VD+L   LFT S F   F E R+  D++  PW     + G + R I YTI++   + PK ++  E Q +H  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:  112 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDF--NTMGYCEEIPAEDNEANDNCNKTPDAKMDISPQLHRKVFPNSSLNSTDSTDAPLN-----------FDVPPVEDFTNCIPEDLLPLTLPDNKLSETGGSAHVPAPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFQEDLNGKLYINEVYKFSVDKLHDILFTESQFMREFLEQRRFSDVVYHPW--RKEDTGNQTREIMYTISLSNPLAPKTATVTETQTLHKASQVSECYIIDAEVIAHDVPYHDYFYTLNRYMLTRVARNKCRLRVSAELRYRKQPWGLVKGFIERNFWSGLDEYFRYLELEL 556          
BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 189.889 bits (481), Expect = 4.534e-50
Identity = 169/665 (25.41%), Postives = 300/665 (45.11%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YI----------------SEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHP------DEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-----STSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRA------WXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKA--NLENLDDEIKSNSFHF 2279
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+                +E+   N   N + +  M  S  L R   P+  +  NS   TD  L   N + P      + I + L P+     K  +          +   + N N +     S S  + +E  S+ R + L    ++    E  + +L+++E     VD+L   LFT S F   F E R+  D++  PW     + G + R I YTI++   + PK ++  E Q +H  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L      L +   +      AT     +P+    P       S +    ++ ++    +    S      PD A      + +  +L +I  + +  ++       ++  +E   Q+  S   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+D+MR  L+ ++    L + D + +    H+
Sbjct:   92 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDNCNKTPDAKMDISPQLHRKVFPNSSL--NSTDSTDAPL---NFDVPPVEDFTNCIPEDLLPLTLPDNKLSETGGSAHVPAPSPSLDFNDNEDLPTELSDSSETHDEGESVSRLTTLCASGEVQAFQEDLNGKLYINEVYKFSVDKLHDILFTESQFMREFLEQRRFSDVVYHPW--RKEDTGNQTREIMYTISLSNPLAPKTATVTETQTLHKASQVSECYIIDAEVIAHDVPYHDYFYTLNRYMLTRVARNKCRLRVSAELRYRKQPWGLVKGFIERNFWSGLDEYFRYLELELNKVQLVLSEQSPKSKALRTATVRRRKRPLTHMRPQHMDEALSPVTTPEDEKSVIQRIKHVAGSTQTRHMPDHASEGLALYSVSKLLLIISSVLVLLVFLNLMLFYKLWMLEYTAQSLASWQGLRSHESKLPQTQIEWAQLLESQQRYHESELEKWREIIKSSVLLLDEMRESLMKLQKGIGLRDYDSDSEERRSHY 745          
BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 209.92 bits (533), Expect = 2.749e-57
Identity = 176/667 (26.39%), Postives = 309/667 (46.33%), Query Frame = 3
Query:  405 TLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI-----------------------NQKNNLSENEIMKNSEVLLR--------------TEAPSFVVG--DNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS----LPKP-VHRKIPN--------------EPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIK 2228
            +LF     Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T++ K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +                       N  ++ S NE+   +   L+              ++AP+F  G  D  DS  D+ +  +NL       +    V+   V     D  + E L TE S+                     +S  H+  ++   +E  + R +++E    +VD+LFS LFT S F   F E R+  ++I  PW +   ENG + R I YTI +   + PK ++  E Q ++  S    CY+VD  V+    P  D F+ INRY +  +   KS+L++++++ Y K  +   K+ IE    SGL +YF+ L N L          L Q +    ++K++      +P  H +IP+              E  HH  +        ++  +S+   L +  +L             +++ +  + L+ +   LE  T      Q    +D     S  +W  +++ QQ+    E+ +WK +++SS+ L+DQM++ L+ ++
Sbjct:   31 SLFVLSPTYKQRNEDFRKLFKQLPDS-ERLIVDYSCALQR---DILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANL-------EEQKVVLLPPVTSPSLDFNDNEDLPTELSD---------------------SSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKK--EENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAEEKPSHLQSISK-LLLVISCVL-------------VLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQ 649          
BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 209.149 bits (531), Expect = 9.398e-57
Identity = 174/659 (26.40%), Postives = 306/659 (46.43%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI-----------------------NQKNNLSENEIMKNSEVLLR--------------TEAPSFVVG--DNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS----LPKP-VHRKIPN--------------EPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIK 2228
            Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T++ K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +                       N  ++ S NE+   +   L+              ++AP+F  G  D  DS  D+ +  +NL       +    V+   V     D  + E L TE S+                     +S  H+  ++   +E  + R +++E    +VD+LFS LFT S F   F E R+  ++I  PW +   ENG + R I YTI +   + PK ++  E Q ++  S    CY+VD  V+    P  D F+ INRY +  +   KS+L++++++ Y K  +   K+ IE    SGL +YF+ L N L          L Q +    ++K++      +P  H +IP+              E  HH  +        ++  +S+   L +  +L             +++ +  + L+ +   LE  T      Q    +D     S  +W  +++ QQ+    E+ +WK +++SS+ L+DQM++ L+ ++
Sbjct:   87 YKQRNEDFRKLFKQLPDS-ERLIVDYSCALQR---DILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANL-------EEQKVVLLPPVTSPSLDFNDNEDLPTELSD---------------------SSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKK--EENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAEEKPSHLQSISK-LLLVISCVL-------------VLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQ 697          
BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 208.764 bits (530), Expect = 1.216e-56
Identity = 174/659 (26.40%), Postives = 306/659 (46.43%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI-----------------------NQKNNLSENEIMKNSEVLLR--------------TEAPSFVVG--DNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS----LPKP-VHRKIPN--------------EPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIK 2228
            Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T++ K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +                       N  ++ S NE+   +   L+              ++AP+F  G  D  DS  D+ +  +NL       +    V+   V     D  + E L TE S+                     +S  H+  ++   +E  + R +++E    +VD+LFS LFT S F   F E R+  ++I  PW +   ENG + R I YTI +   + PK ++  E Q ++  S    CY+VD  V+    P  D F+ INRY +  +   KS+L++++++ Y K  +   K+ IE    SGL +YF+ L N L          L Q +    ++K++      +P  H +IP+              E  HH  +        ++  +S+   L +  +L             +++ +  + L+ +   LE  T      Q    +D     S  +W  +++ QQ+    E+ +WK +++SS+ L+DQM++ L+ ++
Sbjct:   84 YKQRNEDFRKLFKQLPDS-ERLIVDYSCALQR---DILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANL-------EEQKVVLLPPVTSPSLDFNDNEDLPTELSD---------------------SSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKK--EENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAEEKPSHLQSISK-LLLVISCVL-------------VLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQ 694          
BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 208.764 bits (530), Expect = 1.524e-56
Identity = 174/659 (26.40%), Postives = 306/659 (46.43%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI-----------------------NQKNNLSENEIMKNSEVLLR--------------TEAPSFVVG--DNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS----LPKP-VHRKIPN--------------EPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIK 2228
            Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T++ K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +                       N  ++ S NE+   +   L+              ++AP+F  G  D  DS  D+ +  +NL       +    V+   V     D  + E L TE S+                     +S  H+  ++   +E  + R +++E    +VD+LFS LFT S F   F E R+  ++I  PW +   ENG + R I YTI +   + PK ++  E Q ++  S    CY+VD  V+    P  D F+ INRY +  +   KS+L++++++ Y K  +   K+ IE    SGL +YF+ L N L          L Q +    ++K++      +P  H +IP+              E  HH  +        ++  +S+   L +  +L             +++ +  + L+ +   LE  T      Q    +D     S  +W  +++ QQ+    E+ +WK +++SS+ L+DQM++ L+ ++
Sbjct:   85 YKQRNEDFRKLFKQLPDS-ERLIVDYSCALQR---DILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANL-------EEQKVVLLPPVTSPSLDFNDNEDLPTELSD---------------------SSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKK--EENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAEEKPSHLQSISK-LLLVISCVL-------------VLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQ 695          
BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 209.149 bits (531), Expect = 4.427e-56
Identity = 174/659 (26.40%), Postives = 305/659 (46.28%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI-----------------------NQKNNLSENEIMKNSEVLLR--------------TEAPSFVVG--DNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS----LPKP-VHRKIPN--------------EPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIK 2228
            Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T++ K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +                       N  ++ S NE+   +   L+              ++AP+F  G  D  DS  D+ +  +NL       +    V+   V     D  + E L TE S+                     +S  H+  ++   +E  + R +++E    +VD+LFS LFT S F   F E R+  ++I  PW     ENG + R I YTI +   + PK ++  E Q ++  S    CY+VD  V+    P  D F+ INRY +  +   KS+L++++++ Y K  +   K+ IE    SGL +YF+ L N L          L Q +    ++K++      +P  H +IP+              E  HH  +        ++  +S+   L +  +L             +++ +  + L+ +   LE  T      Q    +D     S  +W  +++ QQ+    E+ +WK +++SS+ L+DQM++ L+ ++
Sbjct:  211 YKQRNEDFRKLFKQLPDS-ERLIVDYSCALQR---DILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANL-------EEQKVVLLPPVTSPSLDFNDNEDLPTELSD---------------------SSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPW--KKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAEEKPSHLQSISK-LLLVISCVL-------------VLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQ 821          
BLAST of VASt domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 207.223 bits (526), Expect = 5.580e-56
Identity = 148/494 (29.96%), Postives = 235/494 (47.57%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRK 1829
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW     ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L    K    +L +I+      K S    V R+
Sbjct:  118 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLT---STGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPW--KKEENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETEL---TKTESTYLAEIHRQSPKEKASKSSAVRRR 591          
BLAST of VASt domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 206.453 bits (524), Expect = 5.958e-56
Identity = 148/494 (29.96%), Postives = 236/494 (47.77%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRK 1829
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L    K    +L +I+      K S    V R+
Sbjct:   51 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLT---STGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETEL---TKTESTYLAEIHRQSPKEKASKSSAVRRR 524          
BLAST of VASt domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 206.453 bits (524), Expect = 8.092e-56
Identity = 148/494 (29.96%), Postives = 235/494 (47.57%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRK 1829
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW     ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L    K    +L +I+      K S    V R+
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLT---STGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPW--KKEENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETEL---TKTESTYLAEIHRQSPKEKASKSSAVRRR 564          
BLAST of VASt domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 206.068 bits (523), Expect = 1.059e-55
Identity = 148/494 (29.96%), Postives = 236/494 (47.77%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRK 1829
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L    K    +L +I+      K S    V R+
Sbjct:   87 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLT---STGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETEL---TKTESTYLAEIHRQSPKEKASKSSAVRRR 560          
BLAST of VASt domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 207.608 bits (527), Expect = 1.657e-55
Identity = 146/476 (30.67%), Postives = 231/476 (48.53%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW     ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L K E+  L +  RQ
Sbjct:  235 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTS---TGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPW--KKEENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETELTKTESTYLAEIHRQ 693          
BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Match: sp|Q3KR37|ASTRB_HUMAN (Protein Aster-B OS=Homo sapiens OX=9606 GN=GRAMD1B PE=1 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 5.210e-55
Identity = 150/471 (31.85%), Postives = 239/471 (50.74%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNIN-----QKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRV-------KRHKFDSPNAESLSTEDSNVNS---NFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL-KHEAKGLGQHLRQ 1772
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N     ++  + ENE+  +S        P         S T+ +L S+  +      D L P+ +  +       K    D+   E +    + VNS   +FN      T  S+S    S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L + L K E+  L +  RQ
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGL-PLEEEALEGDGSLEKELAIDNIMGEKIEM-IAPVNSPSLDFNDNEDIPTELSDS----SDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQ 549          
BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Match: sp|Q80TI0|ASTRB_MOUSE (Protein Aster-B OS=Mus musculus OX=10090 GN=Gramd1b PE=1 SV=2)

HSP 1 Score: 206.068 bits (523), Expect = 8.416e-55
Identity = 148/494 (29.96%), Postives = 236/494 (47.77%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDS-PNAESLSTEDSNVNSNFNSTSL---------PSTNES----------NSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRK 1829
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T S K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E  I +N      +++      D S     +S+ +S L      G    PV         FD  P  E +   D ++       ++         P T+ S            +  +S  H+  ++   +E    R +V+E  +  VD+L+  LFTNS F   F E R+  DII  PW +   ENG + R I YTIT+   + PK ++  E Q ++  S    CY++D  V     P  D F+ INRY +  +   KS+L+V++++ Y K  +   K+ IE    SGL DYF  L   L    K    +L +I+      K S    V R+
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLT---STGSSEAPV--------SFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPWKKE--ENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETEL---TKTESTYLAEIHRQSPKEKASKSSAVRRR 564          
BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Match: sp|Q3KR56|ASTRA_RAT (Protein Aster-A OS=Rattus norvegicus OX=10116 GN=Gramd1a PE=2 SV=2)

HSP 1 Score: 205.297 bits (521), Expect = 1.130e-54
Identity = 153/519 (29.48%), Postives = 245/519 (47.21%), Query Frame = 3
Query:  378 LNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEE---CNINQKNNLSENEI--------MKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDS---NVNSNFNSTSLPSTNE------------SNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFN----DLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEP 1844
            + N K+ +S        Y+ +   F+K+FS    + ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  I + +  ++ + KEKT K+IPNAIQI T+S K+FFTSFG+R++ F+++  L       + L+  +   L+H CYGSE  +   +E   C +      S  E+        +  S    R++ PS V         + S  SS+ +H  E         +      +  +P AE LS E +      SN     L S  E                   +LL +++          RL ++    +  +RL   LF++S F   F + RK  D+  +PW   ++    + R + YTI +  ++GPK +S  E Q +          +VD+ V   G P  D F+  +RYCI+ L   K++L+V+S++ Y K  +   KSLIE ++ +G+ DYF+    +LA + K   +  G+  R +       K  L    HR  P  P
Sbjct:   71 IRNSKKMQSWYSMLCPTYKQRNEDFRKLFSKLPEA-ERLIVDYSCALQRE---ILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDVIALSDISPSGAADRSQEPSPVGSRCGRVTPNLSRASSDADHGAEEDKEDQTDSQLDASSSQTVTPVAEPLSAEPAPPDGPTSNLGPLDLLSREELLTDTSNSSSSTGEEGDLAALLPDLS---------GRLLINSVFHVGAERLQQMLFSDSPFLQGFLQQRKFTDVTLSPW--SSDSKCHQRRVLTYTIPISNQLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKSSWTGIEDYFHHLDRELAKAEKVSLEEGGKDARGLLSGLRRRKRPLSWRGHRDGPQHP 574          
BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8VEF1|ASTRA_MOUSE (Protein Aster-A OS=Mus musculus OX=10090 GN=Gramd1a PE=1 SV=2)

HSP 1 Score: 204.527 bits (519), Expect = 2.749e-54
Identity = 156/524 (29.77%), Postives = 246/524 (46.95%), Query Frame = 3
Query:  378 LNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEE---CNINQKNNLSENEI--------MKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNH-----PDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDS---NVNSNFNSTSLPSTNE------------SNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFN----DLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEP 1844
            + N K+ +S        Y+ +   F+K+FS    + ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  I + +  ++ + KEKT K+IPNAIQI T+S K+FFTSFG+R++ F+++  L       + L+  +   L+H CYGSE  +   +E   C +      S  E+        +  S     ++ PS V         + S  SS+ +H      +E  D L       V      +P AE LS+E +      S+     L S  E                   +LL +++          RL ++    M  +RL   LF++S F   F + RK  D+  +PW   ++    + R + YTI +  ++GPK +S  E Q +          +VD+ V   G P  D F+  +RYCI+ L   K++L+V+S++ Y K  +   KSLIE  + SG+ DYF+    +LA + K   +  G+  R +       K  L    HR  P  P
Sbjct:   71 IRNSKKMQSWYSMLCPTYKQRNEDFRKLFSKLPEA-ERLIVDYSCALQRE---ILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDVIALSDISPSGAADHSQEPSPVGSRRGRVTPNLSRASSDADHGAEEDKEEQTDGLDASSSQTV------TPVAEPLSSEPTPPDGPTSSLGPLDLLSREELLTDTSNSSSSTGEEGDLAALLPDLS---------GRLLINSVFHMGAERLQQMLFSDSPFLQGFLQQRKFTDVTLSPW--SSDSKCHQRRVLTYTIPISNQLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLDRELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRGHRDGPQHP 573          
BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8IYS0|ASTRC_HUMAN (Protein Aster-C OS=Homo sapiens OX=9606 GN=GRAMD1C PE=1 SV=2)

HSP 1 Score: 201.06 bits (510), Expect = 1.605e-53
Identity = 132/450 (29.33%), Postives = 216/450 (48.00%), Query Frame = 3
Query:  486 DQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNI--------------------NQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKR---HKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHL 1766
            ++L+ DY+CAL +    IL+QGRLY++E W+CFYS I  +E  I + +  I+ ++KEKT ++IPNAIQI T+S K+FFTSFG+R++S++ +  L       ++L   +   L+   YG+E  +   E  N+                     +++      I   SE + R        G++S       L      +  +    L P ++ ++ R      D    E LS + S+ + + +  ++P  +          LH             RLF++    +  DR+F  LFT+S F  +F   R   D++  PW   A   G + RT+ YTI + + +  K +++ EKQ ++  S     Y+VD+ V     P  D F+ +NRYCII  +  K +L+V++ + Y K  +   KSLIE  + S L DYF  L + L  E   L Q +
Sbjct:   82 ERLIADYACALQRD---ILLQGRLYLSENWLCFYSNIFRWETTISIALKNITFMTKEKTARLIPNAIQIVTESEKFFFTSFGARDRSYLSIFRLWQNVLLDKSLTRQEFWQLLQQNYGTELGLNAEEMENLSLSIEDVQPRSPGRSSLDDSGERDEKLSKSISFTSESISRVSETESFDGNSSKG----GLGKEESQNEKQTKKSLLPTLEKKLTRVPSKSLDLNKNEYLSLDKSSTSDSVDEENVPEKD----------LHG------------RLFINRIFHISADRMFELLFTSSRFMQKFASSRNIIDVVSTPWT--AELGGDQLRTMTYTIVLNSPLTGKCTAATEKQTLYKESREARFYLVDSEVLTHDVPYHDYFYTVNRYCIIRSSKQKCRLRVSTDLKYRKQPWGLVKSLIEKNSWSSLEDYFKQLESDLLIEESVLNQAI 500          
BLAST of VASt domain-containing protein vs. TrEMBL
Match: V4AF14 (VASt domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_104744 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 4.937e-84
Identity = 203/629 (32.27%), Postives = 319/629 (50.72%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEI-GDHL--HPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEA----KGLGQHL---RQINVNHGATKVSLP----KPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITN---------IEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLEN 2243
             Y+++   + KIF D    +++L+ DYSCAL K    IL+QGR+Y+++KWICFY+ I  +E  + +P S I+ ++KEKT +VIPNAIQITT+  KYFFTSFG+R+K++++L  L       E +N  ++   IH  YG E  +  S+E  +       ++ IMK+   L   E P  +  D+SD      + +   N  D I  D +   P +         +SP   +++TE   + ++F  T+  S  E            VA  C+ H+H  +L+VDE  +M++D++F+ LFT++ F+  F E RKTFD+    W E  +E+G + RTI+YT+++   IGPK S S E+Q  +  S PG  Y+VD    N G P +D F  + +YC+  +   K +L+VT ++ Y KS+    KS+I+ +A  GLTDYF DLA  L+ E     K LG  +   +        T  S P    +   R+I + P+ H+SK  K          S  F +    +L                       Y +   LE  T+         I  N   +P       +S  +W  ++K+QQ +   EI +W+ ++ +SI L+DQM+  LV +  +LE 
Sbjct:    9 TYKSRSEDYHKIFKDV-PKDERLVVDYSCALQKD---ILVQGRMYISQKWICFYANIFRWETVLCIPCSEITAITKEKTARVIPNAIQITTEKEKYFFTSFGARDKTYMMLFRLWQNALLSEPMNPKEQWQWIHYSYGEELGLTSSDEDYVAPPG--MDDLIMKDK--LSSPEMPPELT-DSSDLHGTSEMLTMASNDEDPIPSDEVFSQPGLS-------LESPLPLNVTTE---IPTDFGDTTEDSEGE------------VA--CSGHDHLEKLYVDEVFNMNIDKMFTLLFTDTPFYRIFVESRKTFDLDIPSWGEEVDEDGNRVRTIHYTLSLNHSIGPKTSPSSERQTCYKQSKPGIIYVVDCQCENNGIPYADSFFVVTQYCLTRVTKEKCRLRVTGEIKYRKSVMGIVKSMIDRSAVGGLTDYFRDLAVHLRREVEENEKLLGHTIAKKKLRKRRKPTTTTSEPLVTSRQTDRQISSPPSPHRSKAQK---------FSLLFLVLCNAVL-----------------------YYKLWSLEHYTSNIYSSNSAQILDNLAKYP-------ESREEWFKLLKQQQLLHDSEIDKWREVLSASIVLVDQMKGTLVHLHDSLEG 565          
BLAST of VASt domain-containing protein vs. TrEMBL
Match: H2KT67 (GRAM domain-containing protein 1B OS=Clonorchis sinensis OX=79923 GN=CLF_112093 PE=4 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 7.708e-72
Identity = 174/565 (30.80%), Postives = 290/565 (51.33%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQI--TTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGD------------NSDSDTDQSLYSSNLNHPDEIG---DHLHP--VIKHRVK----------RHKFDSPN-----AESLSTE-DSNVNSNFNSTSL-------------PSTNESNSMYRTSLL--------------HNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWP------EHANENGTK-----ERTIYYTITMKAKIGPKVSSSEEKQ-IIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS---LPKPVHRKIPNEPTHHQSKINKTMNDLN 1889
            +Y++K+  F+++F  +    ++LL DYSCAL+K+ HG+L QGR+Y+TE W+CFYSKI+S E KI L +  I  ++KEKT +VIPNAIQI  +    ++FFTSF SRE++  +L+ +    +N ++++  +    + + YG ++   + +E + +   +  EN           +  P+    D             SD+  +QS   S     DE G   D LH   V+   V+          R +  +P+     A+S +TE D  ++   ++  L             PS  + +S    S L               +V      H+H  +L+ D  I ++VD LF+C+FT+S FF R +  R TF++ Q+ WP         + + T+     ERTI YT+ +  K+GP+  ++ E+Q ++ + S PG CY+VD  V N   PL +CF  ++RYC++ ++   S+L+V+S+VVY+K +FF +KS+IE+T +SGLTD+F  L   L   A  L +  R       A   +   +   V  + P +    +S++N  + D+N
Sbjct:   63 SYKSKLEQFRRVFRGTPVDTERLLVDYSCALSKNNHGLLQQGRMYITENWVCFYSKIIS-EQKIYLAVKEIIAITKEKTARVIPNAIQIIYSKNHQRFFFTSFSSRERTHAILRKVWENCRNNQSMSIDEIVQQVREIYGDDSLAMLEDE-DTDGDPDTRENPFPSRIRTTSDSSDPNLTSADTANNANVDATMWGSDNGANQSGGLSGFATTDEEGFNEDGLHRDRVLDFDVQVLPSSMEVGIRRRCHTPDLRHNAADSAATETDPALHETHSAGQLSMKSERKRRSKKRPSRTQPSSGIPPSQLTQFTLSPSVSAEGPDSVVTCFPGHDHPGQLYADALIPLNVDALFTCIFTDSVFFDRLSTARGTFNLAQSSWPTVNWSKSERDMSATEPIVNPERTIGYTLKLNQKLGPRTCAAIEQQTLLVSESQPGRCYVVDAKVTNQAVPLCNCFCVVSRYCLLRIDRTNSRLRVSSRVVYEKPVFFGAKSIIENTCRSGLTDFFTALVTHLTEMASALTEEERLTGACLSAQSTNSAVIHDGVRSRNPAKIVAQRSQLNGELGDIN 625          
BLAST of VASt domain-containing protein vs. TrEMBL
Match: A0A4S2LYV8 (VASt domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_003941 PE=4 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 1.767e-71
Identity = 211/728 (28.98%), Postives = 349/728 (47.94%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQI--TTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGD------------NSDSDTDQSLYSSNLNHPDEIG---DHLH------------PVIKHRVKRHKFDSPN-----AESLSTE-DSNVNSNFNSTSL-------------PSTNESNSMYRTSLL--------------HNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWP------EHANENGTK-----ERTIYYTITMKAKIGPKVSSSEEKQ-IIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLR---------------------------------QINVNHGAT-KVSLPKPVHR---KIPNEPT---HHQSKINKTMNDLNMSPMSEDFTLSVGNI----LRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSR--DPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLD 2249
            +Y++K+  F+++F  +    ++LL DYSCAL+K+ HG+L QGR+Y+TE W+CFYSKI+S E KI L +  I  ++KEKT +VIPNAIQI  +    ++FFTSF SRE++  +L+ +    +N ++++  +    + + YG ++ + + E+ + +   +  EN           +  P+    D             SD+  + S   S     DE G   D LH            P       R +  +P+     A+S +TE D  ++   ++  L             PS  + +S    S L               +V      H+H  +L+ D  I ++VD LF+C+FT+S FF R +  R TF++ Q+ WP           + T+     ERTI YT+ +  K+GP+  ++ E+Q ++   S PG CY++D  V N   PL +CF  ++RYC++ ++   S+L+V+S+VVY+K +FF +KS+IE+T +SGLTD+F  L   L   A  L +  R                                 Q+N   G T  V + +   R   K+P  P     H+ +   ++N  + +P+    + S+  I    LRPDRAWL+L+++SL++CLCLS +Y+R   LE +   +++    P++   P   D                   +   K L  S    + Q++NVL  +   +E LD
Sbjct:   63 SYKSKLEQFRRVFRGTPVDTERLLVDYSCALSKNNHGLLQQGRMYITENWVCFYSKIIS-EQKIYLAVKEIIAITKEKTARVIPNAIQIIYSKNHQRFFFTSFSSRERTHAILRKVWENCRNNQSMSIDEIVQQVREIYGDDS-LAMLEDEDTDGDPDTRENPFPSRVRTTSDSSDPNLTSADTANNANVDANMWGSDNGANNSGGLSGFATTDEEGFNEDGLHHDRVLDFDVQVLPSSMELGTRRRCHTPDLRHNAADSGATETDPALHETHSAGQLSMKSERKRRSKKRPSRTQPSSGIPPSQLTQFTISPSVSAEGPDSVVTCFPGHDHPGQLYADALIPLNVDALFTCIFTDSIFFDRLSTARGTFNLTQSSWPTVNWSKPEREMSATEPIVNPERTIGYTLKLNQKLGPRTCAAIEQQTLLVGESQPGRCYVIDAKVTNQAVPLCNCFCVVSRYCLLRIDRTNSRLRVSSRVVYEKPVFFGAKSIIENTCRSGLTDFFTALVTHLTEMASALTEEERLTGACLSAQSTNSSVIHDGVRSRNPAKLVAQRGQLNGELGDTNPVDVARKRGRSDAKLPTSPASSPSHKRQFKSSVNPPSNAPVFIAQSASLTEIGYFFLRPDRAWLLLVVVSLLLCLCLSMVYNRLVALEHLAE-QLSEAQSPTKFVGPGFMDQLSSSFTRSPTSFDASQSRLRVMKELTISMSQTLAQIQNVLSQVHHTIELLD 787          
BLAST of VASt domain-containing protein vs. TrEMBL
Match: A0A4S2LYR0 (VASt domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_003941 PE=4 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 2.261e-71
Identity = 215/748 (28.74%), Postives = 357/748 (47.73%), Query Frame = 3
Query:  366 NTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQI--TTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGD------------NSDSDTDQSLYSSNLNHPDEIG---DHLH------------PVIKHRVKRHKFDSPN-----AESLSTE-DSNVNSNFNSTSL-------------PSTNESNSMYRTSLL--------------HNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWP------EHANENGTK-----ERTIYYTITMKAKIGPKVSSSEEKQ-IIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLR---------------------------------QINVNHGAT-KVSLPKPVHR---KIPNEPT---HHQSKINKTMNDLNMSPMSEDFTLSVGNI----LRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSR--DPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLD 2249
            ++ S  + KR     +S   +Y++K+  F+++F  +    ++LL DYSCAL+K+ HG+L QGR+Y+TE W+CFYSKI+S E KI L +  I  ++KEKT +VIPNAIQI  +    ++FFTSF SRE++  +L+ +    +N ++++  +    + + YG ++ + + E+ + +   +  EN           +  P+    D             SD+  + S   S     DE G   D LH            P       R +  +P+     A+S +TE D  ++   ++  L             PS  + +S    S L               +V      H+H  +L+ D  I ++VD LF+C+FT+S FF R +  R TF++ Q+ WP           + T+     ERTI YT+ +  K+GP+  ++ E+Q ++   S PG CY++D  V N   PL +CF  ++RYC++ ++   S+L+V+S+VVY+K +FF +KS+IE+T +SGLTD+F  L   L   A  L +  R                                 Q+N   G T  V + +   R   K+P  P     H+ +   ++N  + +P+    + S+  I    LRPDRAWL+L+++SL++CLCLS +Y+R   LE +   +++    P++   P   D                   +   K L  S    + Q++NVL  +   +E LD
Sbjct:  180 SSFSRQSSKRLTGWYYSLAPSYKSKLEQFRRVFRGTPVDTERLLVDYSCALSKNNHGLLQQGRMYITENWVCFYSKIIS-EQKIYLAVKEIIAITKEKTARVIPNAIQIIYSKNHQRFFFTSFSSRERTHAILRKVWENCRNNQSMSIDEIVQQVREIYGDDS-LAMLEDEDTDGDPDTRENPFPSRVRTTSDSSDPNLTSADTANNANVDANMWGSDNGANNSGGLSGFATTDEEGFNEDGLHHDRVLDFDVQVLPSSMELGTRRRCHTPDLRHNAADSGATETDPALHETHSAGQLSMKSERKRRSKKRPSRTQPSSGIPPSQLTQFTISPSVSAEGPDSVVTCFPGHDHPGQLYADALIPLNVDALFTCIFTDSIFFDRLSTARGTFNLTQSSWPTVNWSKPEREMSATEPIVNPERTIGYTLKLNQKLGPRTCAAIEQQTLLVGESQPGRCYVIDAKVTNQAVPLCNCFCVVSRYCLLRIDRTNSRLRVSSRVVYEKPVFFGAKSIIENTCRSGLTDFFTALVTHLTEMASALTEEERLTGACLSAQSTNSSVIHDGVRSRNPAKLVAQRGQLNGELGDTNPVDVARKRGRSDAKLPTSPASSPSHKRQFKSSVNPPSNAPVFIAQSASLTEIGYFFLRPDRAWLLLVVVSLLLCLCLSMVYNRLVALEHLAE-QLSEAQSPTKFVGPGFMDQLSSSFTRSPTSFDASQSRLRVMKELTISMSQTLAQIQNVLSQVHHTIELLD 924          
BLAST of VASt domain-containing protein vs. TrEMBL
Match: A0A419PLM0 (GRAM domain-containing protein 1B OS=Clonorchis sinensis OX=79923 GN=Gramd1b PE=4 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.050e-70
Identity = 174/565 (30.80%), Postives = 290/565 (51.33%), Query Frame = 3
Query:  426 NYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQI--TTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGD------------NSDSDTDQSLYSSNLNHPDEIG---DHLHP--VIKHRVK----------RHKFDSPN-----AESLSTE-DSNVNSNFNSTSL-------------PSTNESNSMYRTSLL--------------HNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWP------EHANENGTK-----ERTIYYTITMKAKIGPKVSSSEEKQ-IIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVS---LPKPVHRKIPNEPTHHQSKINKTMNDLN 1889
            +Y++K+  F+++F  +    ++LL DYSCAL+K+ HG+L QGR+Y+TE W+CFYSKI+S E KI L +  I  ++KEKT +VIPNAIQI  +    ++FFTSF SRE++  +L+ +    +N ++++  +    + + YG ++   + +E + +   +  EN           +  P+    D             SD+  +QS   S     DE G   D LH   V+   V+          R +  +P+     A+S +TE D  ++   ++  L             PS  + +S    S L               +V      H+H  +L+ D  I ++VD LF+C+FT+S FF R +  R TF++ Q+ WP         + + T+     ERTI YT+ +  K+GP+  ++ E+Q ++ + S PG CY+VD  V N   PL +CF  ++RYC++ ++   S+L+V+S+VVY+K +FF +KS+IE+T +SGLTD+F  L   L   A  L +  R       A   +   +   V  + P +    +S++N  + D+N
Sbjct:  266 SYKSKLEQFRRVFRGTPVDTERLLVDYSCALSKNNHGLLQQGRMYITENWVCFYSKIIS-EQKIYLAVKEIIAITKEKTARVIPNAIQIIYSKNHQRFFFTSFSSRERTHAILRKVWENCRNNQSMSIDEIVQQVREIYGDDSLAMLEDE-DTDGDPDTRENPFPSRIRTTSDSSDPNLTSADTANNANVDATMWGSDNGANQSGGLSGFATTDEEGFNEDGLHRDRVLDFDVQVLPSSMEVGIRRRCHTPDLRHNAADSAATETDPALHETHSAGQLSMKSERKRRSKKRPSRTQPSSGIPPSQLTQFTLSPSVSAEGPDSVVTCFPGHDHPGQLYADALIPLNVDALFTCIFTDSVFFDRLSTARGTFNLAQSSWPTVNWSKSERDMSATEPIVNPERTIGYTLKLNQKLGPRTCAAIEQQTLLVSESQPGRCYVVDAKVTNQAVPLCNCFCVVSRYCLLRIDRTNSRLRVSSRVVYEKPVFFGAKSIIENTCRSGLTDFFTALVTHLTEMASALTEEERLTGACLSAQSTNSAVIHDGVRSRNPAKIVAQRSQLNGELGDIN 828          

HSP 2 Score: 83.1889 bits (204), Expect = 1.768e-12
Identity = 36/79 (45.57%), Postives = 55/79 (69.62%), Query Frame = 3
Query:  390 KRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILP 626
            KR     +S   +Y++K+  F+++F  +    ++LL DYSCAL+K+ HG+L QGR+Y+TE W+CFYSKI+S E K + P
Sbjct:  188 KRLTGWYYSLAPSYKSKLEQFRRVFRGTPVDTERLLVDYSCALSKNNHGLLQQGRMYITENWVCFYSKIIS-EQKSLAP 265          
BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Match: gramd1ba (GRAM domain-containing protein 1B-like [Source:NCBI gene;Acc:103036148])

HSP 1 Score: 195.282 bits (495), Expect = 3.504e-52
Identity = 132/451 (29.27%), Postives = 220/451 (48.78%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLST---EDSNVNSNFNSTSLPST----NE--SNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E     N      T++P   V  ++     +   S+NL+     G    PV        +F     E L T    D+ ++ +      PS     NE     +  +S  H+  ++   HE  + + +++E     VD+L+  LFT S F   F E R+  D++  PW     E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:   61 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDACTKSPEVKV--DASPQLPKIFTSNNLS---STGSTDTPVHYDLAPEEEFTGCLPEDLLTLPLPDNKLSESSAPVPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFHEDLNGKQYINEIYKFSVDKLYDLLFTESQFMRDFLELRRFSDVVYRPW--KKEETGDQSREIMYTISLTNPLAPKTATVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYVLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIERNFWSGLDEYFRHLELEL 500          
BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Match: gramd1ba (GRAM domain-containing protein 1B-like [Source:NCBI gene;Acc:103036148])

HSP 1 Score: 195.282 bits (495), Expect = 3.504e-52
Identity = 132/451 (29.27%), Postives = 220/451 (48.78%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLST---EDSNVNSNFNSTSLPST----NE--SNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E     N      T++P   V  ++     +   S+NL+     G    PV        +F     E L T    D+ ++ +      PS     NE     +  +S  H+  ++   HE  + + +++E     VD+L+  LFT S F   F E R+  D++  PW     E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:   61 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDACTKSPEVKV--DASPQLPKIFTSNNLS---STGSTDTPVHYDLAPEEEFTGCLPEDLLTLPLPDNKLSESSAPVPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFHEDLNGKQYINEIYKFSVDKLYDLLFTESQFMRDFLELRRFSDVVYRPW--KKEETGDQSREIMYTISLTNPLAPKTATVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYVLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIERNFWSGLDEYFRHLELEL 500          
BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Match: gramd1ba (GRAM domain-containing protein 1B-like [Source:NCBI gene;Acc:103036148])

HSP 1 Score: 195.282 bits (495), Expect = 4.919e-52
Identity = 132/451 (29.27%), Postives = 220/451 (48.78%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLST---EDSNVNSNFNSTSLPST----NE--SNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E     N      T++P   V  ++     +   S+NL+     G    PV        +F     E L T    D+ ++ +      PS     NE     +  +S  H+  ++   HE  + + +++E     VD+L+  LFT S F   F E R+  D++  PW     E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:   97 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDACTKSPEVKV--DASPQLPKIFTSNNLS---STGSTDTPVHYDLAPEEEFTGCLPEDLLTLPLPDNKLSESSAPVPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFHEDLNGKQYINEIYKFSVDKLYDLLFTESQFMRDFLELRRFSDVVYRPW--KKEETGDQSREIMYTISLTNPLAPKTATVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYVLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIERNFWSGLDEYFRHLELEL 536          
BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Match: gramd1ba (GRAM domain-containing protein 1B-like [Source:NCBI gene;Acc:103036148])

HSP 1 Score: 194.897 bits (494), Expect = 6.020e-52
Identity = 130/451 (28.82%), Postives = 221/451 (49.00%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLST---EDSNVNSNFNSTSLPSTNESN------SMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E     N      T++P   V  ++     +   S+NL+     G    PV        +F     E L T    D+ ++ +      PS + ++       +  +S  H+  ++   HE  + + +++E     VD+L+  LFT S F   F E R+  D++  PW     E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:   47 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDACTKSPEVKV--DASPQLPKIFTSNNLS---STGSTDTPVHYDLAPEEEFTGCLPEDLLTLPLPDNKLSESSAPVPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFHEDLNGKQYINEIYKFSVDKLYDLLFTESQFMRDFLELRRFSDVVYRPW--KKEETGDQSREIMYTISLTNPLAPKTATVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYVLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIERNFWSGLDEYFRHLELEL 486          
BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Match: gramd1ba (GRAM domain-containing protein 1B-like [Source:NCBI gene;Acc:103036148])

HSP 1 Score: 194.897 bits (494), Expect = 8.088e-52
Identity = 132/451 (29.27%), Postives = 220/451 (48.78%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADI------YISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLST---EDSNVNSNFNSTSLPST----NE--SNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSL 1733
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  + +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +      Y+  + + N      E     N      T++P   V  ++     +   S+NL+     G    PV        +F     E L T    D+ ++ +      PS     NE     +  +S  H+  ++   HE  + + +++E     VD+L+  LFT S F   F E R+  D++  PW     E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L+V++++ Y K  +   K  IE    SGL +YF  L   L
Sbjct:  130 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEDNEANDACTKSPEVKV--DASPQLPKIFTSNNLS---STGSTDTPVHYDLAPEEEFTGCLPEDLLTLPLPDNKLSESSAPVPSPSLDFNDNEDLPTELSDSSETHDEGEVQAFHEDLNGKQYINEIYKFSVDKLYDLLFTESQFMRDFLELRRFSDVVYRPW--KKEETGDQSREIMYTISLTNPLAPKTATVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYVLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIERNFWSGLDEYFRHLELEL 569          
BLAST of VASt domain-containing protein vs. Ensembl Sea Lamprey
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 167.933 bits (424), Expect = 2.160e-45
Identity = 125/460 (27.17%), Postives = 216/460 (46.96%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYG------SEADIYISEECNINQKNNL--SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPD----------EIGDHLHPVIKHRVKRHKFDSPN---AESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITM-KAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHE 1742
            YR +   F++IF      N+ L+ DYSCAL +    IL+ GRLY++E W+CF+S I  +E  + + M  I+ V+KE+T +VIPNAIQ+ T++ KYFF+S G+R+++F+++  L       + L++ +    +   YG      SE D + S         N   S       +    R   P   +G      T  +  S + +  D           +G  + P      +R  + SP      + ST+DS+ +          T   +   R        Q+C          +D E+ +  ++L   LF++S F  RF   RK  D++  PW +   + GT++R++ YTI +      PK SS+ E QI++  S      +V + V     P S+ F   N + +  L++  ++L+++ +V + K  +   K+LI+ ++ +GL +YF  L + L  E
Sbjct:    4 YRQRNDDFRRIFKQL-PENEHLVVDYSCALQRD---ILVHGRLYLSEGWLCFHSNIFRWETLLTIQMKEITAVTKERTARVIPNAIQVCTQNEKYFFSSLGARDRAFLMIFRLWQNSLLDKTLDSREFLQYVQQAYGSDSTLHSEGDEHASRPVRAAVTPNTPSSSAHAPHGTRTPDRAATPDISMGSPRAPPTHLASTSPHGSRGDLSQVDGLTMSMLGREMTP----EPQRRDYPSPELGPGTNPSTDDSDASEG--------TPAGDGADRKPHTLPPGQVC----------IDREVCVGTEKLEKLLFSDSLFMRRFLLARKITDVVCKPWEQ--TKPGTEQRSLTYTINLNGGPFTPKSSSATEVQIMNKES--SDWLVVVSEVATHDVPYSEYFFNRNHFLLQRLSSHTTRLRISWEVCFRKQPWGPVKALIDKSSSTGLAEYFALLDSELLRE 433          
BLAST of VASt domain-containing protein vs. Ensembl Sea Lamprey
Match: gramd1bb (GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GENE-140106-106])

HSP 1 Score: 166.007 bits (419), Expect = 2.335e-43
Identity = 128/481 (26.61%), Postives = 216/481 (44.91%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTE-DSNVNS----------------NFNSTSLPSTNESNSM---------------YRTSLLHNVAQI-CNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRN----GGF--PLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGL 1754
            Y+ +   F+K+F     S ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I+ ++KEKT ++IPNAIQI T++ K+FFTS G+R+++F+++  L       + L   +    +H CYG+E  +   +E  +   ++++    + +S  L      +FVV D           S +LNH      +  P+  +  ++    SP A  +S + D+  N                       +P T    S+                 TS  H   ++ C   +   R+ V+    +  D+LF  LF +  F   F E RK  D    PW + A   G   R + Y + M   + PK ++  E Q+          Y +D+ +      G F  PL +    ++R+ I+        L+V+S++ Y K  +   K+ IE    +G+ DYF  L + +    K L
Sbjct:   14 YKQRNEDFRKLFKQLPES-ERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTIRLKEITAMTKEKTARLIPNAIQICTENDKHFFTSLGARDRTFMMMFRLWQNALLDKPLCPKELWQFVHQCYGNELGLTSEDEDYVPPDDDIATMGGIDSSSSL------AFVVSDLK--------ASPSLNH------NSKPMTVYNARQRSALSPTAARISFDFDTLANGDGDDLGSGDGHSERGLGGGDVPMPQTPSPPSLDMNTNEGIPTELSDSSETSDEHAPCEVDCGMGDLSGRVVVNAVFHLGADKLFDLLFHDPEFGRNFAESRKLLDFCVQPWRKEA--TGHMTRVLTYGVAMSNPLAPKSANGTETQVWQQRPKQQQTYAIDSQILKLDILGPFLPPLWE----VSRFGIVGFAVHGVSLRVSSEIRYRKQPWGLVKTFIERNTWNGIDDYFRHLESEVGRAEKLL 464          
BLAST of VASt domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003678.1 (pep scaffold:Pmarinus_7.0:GL477104:103280:105081:-1 gene:ENSPMAG00000003360.1 transcript:ENSPMAT00000003678.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.4698 bits (158), Expect = 3.896e-13
Identity = 27/88 (30.68%), Postives = 56/88 (63.64%), Query Frame = 3
Query:  504 YSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTL 767
            ++CAL +    ++ QGR++V+E WICF++     + K+ +P+  +  + K+++  ++PNAI IT+   K+ F SF +R+ ++  L ++
Sbjct:   26 FNCALQRD---LIFQGRMFVSENWICFFANFFGKDVKVAIPVLTVKAIKKQRSALLLPNAIGITSACEKHMFISFLARDAAYKALLSV 110          
BLAST of VASt domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006389.1 (pep scaffold:Pmarinus_7.0:GL481951:143257:161898:-1 gene:ENSPMAG00000005741.1 transcript:ENSPMAT00000006389.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 1.189e-9
Identity = 31/115 (26.96%), Postives = 66/115 (57.39%), Query Frame = 3
Query:  423 NNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTL 767
            + +   V  F+K+F   +   ++L+  YSC+  K    +  QG LY++   +CFYS ++  E K+++  + ++ + +  TV V+P ++++ T+ G  FF+ F S  ++F +++ L
Sbjct:   64 DKFMEAVAKFRKLFGMPE--EERLVNYYSCSYWKGR--VPRQGWLYLSVNHVCFYSLLLGKEAKLVVRWADVTCLERTATV-VLPESVRVATRDGDLFFSMFLSVGETFRLMERL 173          
BLAST of VASt domain-containing protein vs. Ensembl Yeast
Match: LAM4 (Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies [Source:SGD;Acc:S000001122])

HSP 1 Score: 81.6481 bits (200), Expect = 2.904e-16
Identity = 41/110 (37.27%), Postives = 67/110 (60.91%), Query Frame = 3
Query:  450 FKKIFSDSDAS-NDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNK 776
            F  IF DS  S N++L+ D+SCAL++    IL+QGR+Y++++ I FYS I+ + + + +P   I  + K  T  + PN I I T   KY F SF SR+ ++ ++  + N+
Sbjct:  550 FHAIFKDSGVSPNERLILDHSCALSRD---ILLQGRMYISDQHIGFYSNILGWVSTVFIPFKTIVQIEKRATAGIFPNGIVIDTLHTKYTFASFTSRDATYDLITEVWNQ 656          
BLAST of VASt domain-containing protein vs. Ensembl Yeast
Match: YSP2 (Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes [Source:SGD;Acc:S000002734])

HSP 1 Score: 78.5666 bits (192), Expect = 2.472e-15
Identity = 39/110 (35.45%), Postives = 67/110 (60.91%), Query Frame = 3
Query:  450 FKKIFSDSDAS-NDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNK 776
            F  +F D D + N++L+ D+SCAL++    IL+QGR+Y+++  I F+S I+ + + + +P   I  + K+ T  + PN I I T   KY F SF SR+ +F ++  + N+
Sbjct:  649 FHTLFKDCDINPNEKLIVDHSCALSRD---ILLQGRMYISDAHIGFFSNILGWVSTVFIPFKEIVQIEKKTTAGIFPNGIVIDTLHTKYIFASFMSRDATFDLITDVWNQ 755          
BLAST of VASt domain-containing protein vs. Ensembl Yeast
Match: YFL042C_mRNA (Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites [Source:SGD;Acc:S000001852])

HSP 1 Score: 74.3294 bits (181), Expect = 3.776e-14
Identity = 47/148 (31.76%), Postives = 78/148 (52.70%), Query Frame = 3
Query:  390 KRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQ-----TLLNKHKNCEALNNTQKKS 818
            +R   T +   +  RNK   F + F  S   +D+LL D++C L +    +L QG+LY+TE  +CF S ++ +  K+++    ++ + K     + P+AI I TK GK  F  F SR+ +F +++     TLL K    E +N   +KS
Sbjct:  182 ERYMDTPYHYASEQRNK--DFHETFK-SVPKDDRLLDDFNCGLNRE---LLYQGKLYITETHLCFNSNVLGWIAKVLIAFEDVTFMEKTSAAGLFPSAISIETKMGKTLFNGFISRDAAFGLMKEVWSRTLLQKDMASENINTKAEKS 323          
BLAST of VASt domain-containing protein vs. Ensembl Yeast
Match: LAM6 (Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes [Source:SGD;Acc:S000004062])

HSP 1 Score: 47.7506 bits (112), Expect = 6.330e-6
Identity = 29/110 (26.36%), Postives = 60/110 (54.55%), Query Frame = 3
Query:  450 FKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKH 779
            F+++F    A N +L+ DY C   +       QGR+Y++   +CF S ++++  K+ +P++ I  + K   V    +AI + T + +Y F+ F +R++ F ++  + +K 
Sbjct:  166 FRQMFKPL-APNTRLITDYFCYFHRE---FPYQGRIYLSNTHLCFNSTVLNWMAKLQIPLNEIKYLDK---VTTNSSAISVETVTNRYTFSGFIARDEVFQLITRVWSKE 268          
BLAST of VASt domain-containing protein vs. Ensembl Nematostella
Match: EDO45283 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTK5])

HSP 1 Score: 58.151 bits (139), Expect = 5.938e-11
Identity = 26/62 (41.94%), Postives = 43/62 (69.35%), Query Frame = 3
Query:  423 NNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFE 608
            ++Y++K   F+++F D   S +QL+ DYSCAL +    IL+ GRLY+++ W+CFY+ I  +E
Sbjct:    3 SSYKSKSGDFRRLFKDLPDS-EQLIVDYSCALQRD---ILVHGRLYISQNWLCFYANIFGWE 60          
BLAST of VASt domain-containing protein vs. Ensembl Nematostella
Match: EDO26770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TB47])

HSP 1 Score: 55.8398 bits (133), Expect = 1.523e-8
Identity = 46/131 (35.11%), Postives = 67/131 (51.15%), Query Frame = 3
Query:  609 NKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIY---ISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQ 992
            N++ +  S + ++ KEKT  VIPNAIQI+T+  KYFF SF SR+ +F VL  +       + L+  +   LIH   G  +D      +EE + N+K+ LS   +   S   L+TE    V      S  DQ
Sbjct:   17 NQVTINCSEVISIRKEKTALVIPNAIQISTEGEKYFFASFISRDTAFTVLFRIWQNALLDQPLSPNE---LIH-MVGKYSDPRHECSTEEDSENEKDKLS---VTPPSPTALKTEQDEGVSATGDSSSLDQ 140          
BLAST of VASt domain-containing protein vs. Ensembl Nematostella
Match: EDO49828 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7REP1])

HSP 1 Score: 54.299 bits (129), Expect = 1.992e-7
Identity = 31/129 (24.03%), Postives = 73/129 (56.59%), Query Frame = 3
Query:  387 PKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLN 773
            P    ST+    + +++    F ++F+  +   D+L+  YSC+  K    +  QG LY++   +CFYS +M  E ++++  + ++++ +  +V ++P+ I++TT+ G++ F+      ++F +++ L N
Sbjct:  124 PDEDTSTIEQETSKFKSASNRFHRLFNMPE--QDKLVNYYSCSYWKGR--VPRQGWLYLSVNHLCFYSFLMGKEARLVIRWTDVTSLERTNSV-LLPDGIKVTTRDGEFCFSLLLHPTETFGLMEQLAN 247          
BLAST of VASt domain-containing protein vs. Ensembl Medaka
Match: gramd1bb (GRAM domain containing 1B [Source:NCBI gene;Acc:101169553])

HSP 1 Score: 186.422 bits (472), Expect = 4.193e-49
Identity = 157/667 (23.54%), Postives = 308/667 (46.18%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNL-------------------------SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-STSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNIL------RPDR--------AWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLE----NLDDEIKSNSFH 2276
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++                          +E +   +   LL+   P+  +    ++DT  +         D + D     +    +  K  S     + +   ++N N +  T L  ++E+         H+  ++   H+  + R  ++E   + VD+L+  LF  S F   F E R+  D++  PW    +E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L++++++ + K  +   K LIE    SGL + F  L   L    + + +   Q++      K S  +   R +P+  + H   +++ ++ +  +P  ED    +  +        PD          + +  +L +I  + +  ++       ++  +E + Q+  +   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+DQM++ L+ ++  +     N + E K + +H
Sbjct:   66 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFSEEIPNEENEINNDNLTKSSAEAKPEGSPPPLLKKVIPNSAIPSPGNNDTPITFDLPVEEFTDCLADGELLTVPLLEEEKKETSGPEGPVPSPSLDLNDNEDIPTELSDSSET---------HDEGEVQAFHDDLNGRQHINEVYRISVDKLYDILFNKSQFMSDFMEQRRFSDVVYHPW--KKDEGGHQTREIMYTISLSNPLAPKTAAVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYMLTRVAKNKCRLRISTELRFRKQPWGLVKGLIEKNFWSGLEENFRHLEMELAKLEEVMNEA-SQLSPKAKGIKNSTVRRKKRPLPHMRSQH---LDEALSPVT-TPTDEDVIQRIKQVAGSTQTRSPDHHHMLGGLALYSVSKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLTTWQSLRLQESKLPQTQMEWAQLLEAQQRYHDAELQKWREIIKSSVVLLDQMKDSLLNLQRGIGLRDYNSETEEKKSRYH 712          
BLAST of VASt domain-containing protein vs. Ensembl Medaka
Match: gramd1bb (GRAM domain containing 1B [Source:NCBI gene;Acc:101169553])

HSP 1 Score: 185.652 bits (470), Expect = 5.541e-49
Identity = 157/666 (23.57%), Postives = 306/666 (45.95%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNL-------------------------SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-STSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNIL------RPDR--------AWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLE----NLDDEIKSNSFH 2276
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++                          +E +   +   LL+   P+  +    ++DT  +         D + D     +    +  K  S     + +   ++N N +  T L  ++E++        H+     N  +H     ++E   + VD+L+  LF  S F   F E R+  D++  PW    +E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L++++++ + K  +   K LIE    SGL + F  L   L    + + +   Q++      K S  +   R +P+  + H   +++ ++ +  +P  ED    +  +        PD          + +  +L +I  + +  ++       ++  +E + Q+  +   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+DQM++ L+ ++  +     N + E K + +H
Sbjct:   59 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFSEEIPNEENEINNDNLTKSSAEAKPEGSPPPLLKKVIPNSAIPSPGNNDTPITFDLPVEEFTDCLADGELLTVPLLEEEKKETSGPEGPVPSPSLDLNDNEDIPTELSDSSETHDEGEVQAFHD---DLNGRQH-----INEVYRISVDKLYDILFNKSQFMSDFMEQRRFSDVVYHPW--KKDEGGHQTREIMYTISLSNPLAPKTAAVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYMLTRVAKNKCRLRISTELRFRKQPWGLVKGLIEKNFWSGLEENFRHLEMELAKLEEVMNE-ASQLSPKAKGIKNSTVRRKKRPLPHMRSQH---LDEALSPVT-TPTDEDVIQRIKQVAGSTQTRSPDHHHMLGGLALYSVSKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLTTWQSLRLQESKLPQTQMEWAQLLEAQQRYHDAELQKWREIIKSSVVLLDQMKDSLLNLQRGIGLRDYNSETEEKKSRYH 705          
BLAST of VASt domain-containing protein vs. Ensembl Medaka
Match: gramd1bb (GRAM domain containing 1B [Source:NCBI gene;Acc:101169553])

HSP 1 Score: 186.422 bits (472), Expect = 6.447e-49
Identity = 157/667 (23.54%), Postives = 308/667 (46.18%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNL-------------------------SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-STSLPSTNESNSMYRTSLLHNVAQICNTHEHFH-RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNIL------RPDR--------AWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLE----NLDDEIKSNSFH 2276
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++                          +E +   +   LL+   P+  +    ++DT  +         D + D     +    +  K  S     + +   ++N N +  T L  ++E+         H+  ++   H+  + R  ++E   + VD+L+  LF  S F   F E R+  D++  PW    +E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L++++++ + K  +   K LIE    SGL + F  L   L    + + +   Q++      K S  +   R +P+  + H   +++ ++ +  +P  ED    +  +        PD          + +  +L +I  + +  ++       ++  +E + Q+  +   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+DQM++ L+ ++  +     N + E K + +H
Sbjct:  129 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFSEEIPNEENEINNDNLTKSSAEAKPEGSPPPLLKKVIPNSAIPSPGNNDTPITFDLPVEEFTDCLADGELLTVPLLEEEKKETSGPEGPVPSPSLDLNDNEDIPTELSDSSET---------HDEGEVQAFHDDLNGRQHINEVYRISVDKLYDILFNKSQFMSDFMEQRRFSDVVYHPW--KKDEGGHQTREIMYTISLSNPLAPKTAAVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYMLTRVAKNKCRLRISTELRFRKQPWGLVKGLIEKNFWSGLEENFRHLEMELAKLEEVMNE-ASQLSPKAKGIKNSTVRRKKRPLPHMRSQH---LDEALSPVT-TPTDEDVIQRIKQVAGSTQTRSPDHHHMLGGLALYSVSKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLTTWQSLRLQESKLPQTQMEWAQLLEAQQRYHDAELQKWREIIKSSVVLLDQMKDSLLNLQRGIGLRDYNSETEEKKSRYH 775          
BLAST of VASt domain-containing protein vs. Ensembl Medaka
Match: gramd1bb (GRAM domain containing 1B [Source:NCBI gene;Acc:101169553])

HSP 1 Score: 185.652 bits (470), Expect = 8.487e-49
Identity = 157/666 (23.57%), Postives = 306/666 (45.95%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNL-------------------------SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-STSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNIL------RPDR--------AWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLE----NLDDEIKSNSFH 2276
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++                          +E +   +   LL+   P+  +    ++DT  +         D + D     +    +  K  S     + +   ++N N +  T L  ++E++        H+     N  +H     ++E   + VD+L+  LF  S F   F E R+  D++  PW    +E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L++++++ + K  +   K LIE    SGL + F  L   L    + + +   Q++      K S  +   R +P+  + H   +++ ++ +  +P  ED    +  +        PD          + +  +L +I  + +  ++       ++  +E + Q+  +   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+DQM++ L+ ++  +     N + E K + +H
Sbjct:   91 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFSEEIPNEENEINNDNLTKSSAEAKPEGSPPPLLKKVIPNSAIPSPGNNDTPITFDLPVEEFTDCLADGELLTVPLLEEEKKETSGPEGPVPSPSLDLNDNEDIPTELSDSSETHDEGEVQAFHD---DLNGRQH-----INEVYRISVDKLYDILFNKSQFMSDFMEQRRFSDVVYHPW--KKDEGGHQTREIMYTISLSNPLAPKTAAVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYMLTRVAKNKCRLRISTELRFRKQPWGLVKGLIEKNFWSGLEENFRHLEMELAKLEEVMNE-ASQLSPKAKGIKNSTVRRKKRPLPHMRSQH---LDEALSPVT-TPTDEDVIQRIKQVAGSTQTRSPDHHHMLGGLALYSVSKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLTTWQSLRLQESKLPQTQMEWAQLLEAQQRYHDAELQKWREIIKSSVVLLDQMKDSLLNLQRGIGLRDYNSETEEKKSRYH 737          
BLAST of VASt domain-containing protein vs. Ensembl Medaka
Match: gramd1bb (GRAM domain containing 1B [Source:NCBI gene;Acc:101169553])

HSP 1 Score: 185.267 bits (469), Expect = 1.396e-48
Identity = 157/666 (23.57%), Postives = 306/666 (45.95%), Query Frame = 3
Query:  429 YRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNL-------------------------SENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFN-STSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNIL------RPDR--------AWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSS------YDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLE----NLDDEIKSNSFH 2276
            Y+ +   F+K+F       ++L+ DYSCAL +    IL+QGRLY++E WICFYS I  +E  + + +  I +++KEKT ++IPNAIQ+ T + K+FFTSFG+R+++++++  L       + L   +    +H CYG+E  +   +E  +   ++                          +E +   +   LL+   P+  +    ++DT  +         D + D     +    +  K  S     + +   ++N N +  T L  ++E++        H+     N  +H     ++E   + VD+L+  LF  S F   F E R+  D++  PW    +E G + R I YTI++   + PK ++  E Q ++  S    CYI+D  V     P  D F+ +NRY +  +   K +L++++++ + K  +   K LIE    SGL + F  L   L    + + +   Q++      K S  +   R +P+  + H   +++ ++ +  +P  ED    +  +        PD          + +  +L +I  + +  ++       ++  +E + Q+  +   LR   S       +W  +++ QQ+    E+ +W+ +++SS+ L+DQM++ L+ ++  +     N + E K + +H
Sbjct:   95 YKQRNEDFRKLFKQL-PDTERLIVDYSCALQRD---ILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLDKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFSEEIPNEENEINNDNLTKSSAEAKPEGSPPPLLKKVIPNSAIPSPGNNDTPITFDLPVEEFTDCLADGELLTVPLLEEEKKETSGPEGPVPSPSLDLNDNEDIPTELSDSSETHDEGEVQAFHD---DLNGRQH-----INEVYRISVDKLYDILFNKSQFMSDFMEQRRFSDVVYHPW--KKDEGGHQTREIMYTISLSNPLAPKTAAVTETQTLYKASQESECYIIDAEVITHDVPYHDYFYTLNRYMLTRVAKNKCRLRISTELRFRKQPWGLVKGLIEKNFWSGLEENFRHLEMELAKLEEVMNE-ASQLSPKAKGIKNSTVRRKKRPLPHMRSQH---LDEALSPVT-TPTDEDVIQRIKQVAGSTQTRSPDHHHMLGGLALYSVSKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLTTWQSLRLQESKLPQTQMEWAQLLEAQQRYHDAELQKWREIIKSSVVLLDQMKDSLLNLQRGIGLRDYNSETEEKKSRYH 741          
BLAST of VASt domain-containing protein vs. Planmine SMEST
Match: SMESG000004391.1 (SMESG000004391.1)

HSP 1 Score: 1415.21 bits (3662), Expect = 0.000e+0
Identity = 755/755 (100.00%), Postives = 755/755 (100.00%), Query Frame = 3
Query:   42 MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTIXXXXXXXXXXXXXXMDTIVHEPDAAEIEFXXXXXXXXXXXXXXXXXXFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS 2306
            MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTINNLLNSQDNSLSNSMDTIVHEPDAAEIEFLSSNLDNSESVSSKSQSSFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWLILIMLSLIICLCLSFLYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWKIMMKKQQQIQIDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS
Sbjct:    1 MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTINNLLNSQDNSLSNSMDTIVHEPDAAEIEFLSSNLDNSESVSSKSQSSFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWLILIMLSLIICLCLSFLYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWKIMMKKQQQIQIDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS 755          
BLAST of VASt domain-containing protein vs. Planmine SMEST
Match: SMESG000004391.1 (SMESG000004391.1)

HSP 1 Score: 1370.14 bits (3545), Expect = 0.000e+0
Identity = 738/755 (97.75%), Postives = 738/755 (97.75%), Query Frame = 3
Query:   42 MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTIXXXXXXXXXXXXXXMDTIVHEPDAAEIEFXXXXXXXXXXXXXXXXXXFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWXXXXXXXXXXXXXXXXXYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWXXXXXXXXXXXXDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS 2306
            MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTINNLLNSQDNSLSNSMDTIVHEPDAAEIEFLSSNLDNSESVSSKSQSSFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWLILIMLSLIICLCLSFLYSRFRQ                 DPLRFDSSYDWKIMMKKQQQIQIDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS
Sbjct:    1 MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPNSPSRDNVSTINNLLNSQDNSLSNSMDTIVHEPDAAEIEFLSSNLDNSESVSSKSQSSFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNEPTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWLILIMLSLIICLCLSFLYSRFRQ-----------------DPLRFDSSYDWKIMMKKQQQIQIDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKGKYTKS 738          
BLAST of VASt domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 258.455 bits (659), Expect = 3.986e-76
Identity = 155/459 (33.77%), Postives = 255/459 (55.56%), Query Frame = 3
Query:  420 INNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSG-KYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMK----NSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHP--DEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNF-NSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQ 1772
            +  Y+ +   FKK F D++A +  L+ DYSCA+   T  IL QGR+Y+T +++CFYS+ ++FE KI +    I  ++KEKT  V  NAIQI T +  K+FF+SF +R++++ V+  L  ++K+   L+N      + + YG++   ++ E+   ++ N        K     SE++  T +P            D SLY S    P  D + D+      H +K   FD     S+  E+   + +F NST +P +N         +    A IC  HEH   + +DEE  ++V  L++ +FT+++FF      R   +I Q+ W +  +    K RTIYYT+ +K +IGPK S + EKQ++   S     Y V+++VR  G P+SD FH + RYC++++N  KS++K++ ++VYDK++FFA+KS+I+S+ KSG  DY  DL  +L  E++ +   L +
Sbjct:    2 LKTYKQRRSDFKKSFKDTNAESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKDGIKLSNAAFWDRVRENYGNDLG-FLLEDVEYDESNATQFTARYKIPRRPSEIMSNTSSP------------DDSLYESLEESPVSDNVIDY-SLCNGHNLKSENFDK-KPPSIPEEEIKFDLSFRNSTQIPESNNIEIPVEDIISSRPAAICADHEHLPNMLLDEEFSINVHTLWTFIFTDNAFFRALCAMRFITEITQSNWSDDVDRE--KCRTIYYTVNLKNRIGPKKSPTVEKQVLLEGSCKDYIYYVESIVRTVGIPMSDSFHIVLRYCLLYINPNKSRIKISMKIVYDKTVFFATKSIIDSSVKSGCIDYHKDLKQALLIESQKINDVLTE 443          
BLAST of VASt domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 258.84 bits (660), Expect = 4.198e-76
Identity = 155/459 (33.77%), Postives = 255/459 (55.56%), Query Frame = 3
Query:  420 INNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSG-KYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMK----NSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHP--DEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNF-NSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQ 1772
            +  Y+ +   FKK F D++A +  L+ DYSCA+   T  IL QGR+Y+T +++CFYS+ ++FE KI +    I  ++KEKT  V  NAIQI T +  K+FF+SF +R++++ V+  L  ++K+   L+N      + + YG++   ++ E+   ++ N        K     SE++  T +P            D SLY S    P  D + D+      H +K   FD     S+  E+   + +F NST +P +N         +    A IC  HEH   + +DEE  ++V  L++ +FT+++FF      R   +I Q+ W +  +    K RTIYYT+ +K +IGPK S + EKQ++   S     Y V+++VR  G P+SD FH + RYC++++N  KS++K++ ++VYDK++FFA+KS+I+S+ KSG  DY  DL  +L  E++ +   L +
Sbjct:    8 LKTYKQRRSDFKKSFKDTNAESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKDGIKLSNAAFWDRVRENYGNDLG-FLLEDVEYDESNATQFTARYKIPRRPSEIMSNTSSP------------DDSLYESLEESPVSDNVIDY-SLCNGHNLKSENFDK-KPPSIPEEEIKFDLSFRNSTQIPESNNIEIPVEDIISSRPAAICADHEHLPNMLLDEEFSINVHTLWTFIFTDNAFFRALCAMRFITEITQSNWSDDVDRE--KCRTIYYTVNLKNRIGPKKSPTVEKQVLLEGSCKDYIYYVESIVRTVGIPMSDSFHIVLRYCLLYINPNKSRIKISMKIVYDKTVFFATKSIIDSSVKSGCIDYHKDLKQALLIESQKINDVLTE 449          
BLAST of VASt domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 258.455 bits (659), Expect = 6.707e-76
Identity = 155/459 (33.77%), Postives = 255/459 (55.56%), Query Frame = 3
Query:  420 INNYRNKVISFKKIFSDSDASNDQLLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGISNVSKEKTVKVIPNAIQITTKSG-KYFFTSFGSREKSFIVLQTLLNKHKNCEALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMK----NSEVLLRTEAPSFVVGDNSDSDTDQSLYSSNLNHP--DEIGDHLHPVIKHRVKRHKFDSPNAESLSTEDSNVNSNF-NSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFHRLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANENGTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGGFPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAKSGLTDYFNDLANSLKHEAKGLGQHLRQ 1772
            +  Y+ +   FKK F D++A +  L+ DYSCA+   T  IL QGR+Y+T +++CFYS+ ++FE KI +    I  ++KEKT  V  NAIQI T +  K+FF+SF +R++++ V+  L  ++K+   L+N      + + YG++   ++ E+   ++ N        K     SE++  T +P            D SLY S    P  D + D+      H +K   FD     S+  E+   + +F NST +P +N         +    A IC  HEH   + +DEE  ++V  L++ +FT+++FF      R   +I Q+ W +  +    K RTIYYT+ +K +IGPK S + EKQ++   S     Y V+++VR  G P+SD FH + RYC++++N  KS++K++ ++VYDK++FFA+KS+I+S+ KSG  DY  DL  +L  E++ +   L +
Sbjct:   17 LKTYKQRRSDFKKSFKDTNAESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKDGIKLSNAAFWDRVRENYGNDLG-FLLEDVEYDESNATQFTARYKIPRRPSEIMSNTSSP------------DDSLYESLEESPVSDNVIDY-SLCNGHNLKSENFDK-KPPSIPEEEIKFDLSFRNSTQIPESNNIEIPVEDIISSRPAAICADHEHLPNMLLDEEFSINVHTLWTFIFTDNAFFRALCAMRFITEITQSNWSDDVDRE--KCRTIYYTVNLKNRIGPKKSPTVEKQVLLEGSCKDYIYYVESIVRTVGIPMSDSFHIVLRYCLLYINPNKSRIKISMKIVYDKTVFFATKSIIDSSVKSGCIDYHKDLKQALLIESQKINDVLTE 458          
The following BLAST results are available for this feature:
BLAST of VASt domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRAMD1B5.153e-5631.85GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
GRAMD1B5.205e-5631.85GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
GRAMD1B6.553e-5631.85GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
GRAMD1B1.064e-5531.85GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
GRAMD1B1.085e-5531.85GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZC328.32.034e-3527.55pep chromosome:WBcel235:I:6409050:6417010:-1 gene:... [more]
ZC328.34.589e-1930.18pep chromosome:WBcel235:I:6409050:6412249:-1 gene:... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GramD1B5.756e-2638.22gene:FBgn0085423 transcript:FBtr0112638[more]
GramD1B6.145e-2636.93gene:FBgn0085423 transcript:FBtr0332409[more]
GramD1B6.474e-2638.22gene:FBgn0085423 transcript:FBtr0112641[more]
GramD1B6.711e-2638.22gene:FBgn0085423 transcript:FBtr0112639[more]
GramD1B7.276e-2638.22gene:FBgn0085423 transcript:FBtr0306333[more]
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BLAST of VASt domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
AL953903.43.553e-5126.01GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
AL953903.49.039e-5128.02GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
AL953903.41.029e-5028.02GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
AL953903.41.519e-5028.02GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
AL953903.44.534e-5025.41GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRAMD1B2.749e-5726.39GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B9.398e-5726.40GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.216e-5626.40GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.524e-5626.40GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B4.427e-5626.40GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gramd1b5.580e-5629.96GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
Gramd1b5.958e-5629.96GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
Gramd1b8.092e-5629.96GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
Gramd1b1.059e-5529.96GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
Gramd1b1.657e-5530.67GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
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BLAST of VASt domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3KR37|ASTRB_HUMAN5.210e-5531.85Protein Aster-B OS=Homo sapiens OX=9606 GN=GRAMD1B... [more]
sp|Q80TI0|ASTRB_MOUSE8.416e-5529.96Protein Aster-B OS=Mus musculus OX=10090 GN=Gramd1... [more]
sp|Q3KR56|ASTRA_RAT1.130e-5429.48Protein Aster-A OS=Rattus norvegicus OX=10116 GN=G... [more]
sp|Q8VEF1|ASTRA_MOUSE2.749e-5429.77Protein Aster-A OS=Mus musculus OX=10090 GN=Gramd1... [more]
sp|Q8IYS0|ASTRC_HUMAN1.605e-5329.33Protein Aster-C OS=Homo sapiens OX=9606 GN=GRAMD1C... [more]
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BLAST of VASt domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V4AF144.937e-8432.27VASt domain-containing protein (Fragment) OS=Lotti... [more]
H2KT677.708e-7230.80GRAM domain-containing protein 1B OS=Clonorchis si... [more]
A0A4S2LYV81.767e-7128.98VASt domain-containing protein OS=Opisthorchis fel... [more]
A0A4S2LYR02.261e-7128.74VASt domain-containing protein OS=Opisthorchis fel... [more]
A0A419PLM01.050e-7030.80GRAM domain-containing protein 1B OS=Clonorchis si... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gramd1ba3.504e-5229.27GRAM domain-containing protein 1B-like [Source:NCB... [more]
gramd1ba3.504e-5229.27GRAM domain-containing protein 1B-like [Source:NCB... [more]
gramd1ba4.919e-5229.27GRAM domain-containing protein 1B-like [Source:NCB... [more]
gramd1ba6.020e-5228.82GRAM domain-containing protein 1B-like [Source:NCB... [more]
gramd1ba8.088e-5229.27GRAM domain-containing protein 1B-like [Source:NCB... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
GRAMD1A2.160e-4527.17GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
gramd1bb2.335e-4326.61GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GE... [more]
ENSPMAT00000003678.13.896e-1330.68pep scaffold:Pmarinus_7.0:GL477104:103280:105081:-... [more]
ENSPMAT00000006389.11.189e-926.96pep scaffold:Pmarinus_7.0:GL481951:143257:161898:-... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
LAM42.904e-1637.27Sterol-binding protein that localizes to puncta in... [more]
YSP22.472e-1535.45Sterol-binding protein; has a probable role in ret... [more]
YFL042C_mRNA3.776e-1431.76Putative sterol transfer protein; one of six StART... [more]
LAM66.330e-626.36Sterol transporter that transfers sterols between ... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO452835.938e-1141.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO267701.523e-835.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498281.992e-724.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of VASt domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gramd1bb4.193e-4923.54GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
gramd1bb5.541e-4923.57GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
gramd1bb6.447e-4923.54GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
gramd1bb8.487e-4923.57GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
gramd1bb1.396e-4823.57GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
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BLAST of VASt domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004391.10.000e+0100.00SMESG000004391.1[more]
SMESG000004391.10.000e+097.75SMESG000004391.1[more]
SMESG000033356.13.986e-7633.77SMESG000033356.1[more]
SMESG000033356.14.198e-7633.77SMESG000033356.1[more]
SMESG000033356.16.707e-7633.77SMESG000033356.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032364 ID=SMED30032364|Name=VASt domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2521bp
TATAAATTTGCCAATTTTACAAAACCCGATCTTTTGATTGGATGGCTGAT
GTATTTCAATGCAATTCATACTCTGACAATACCCAAGATCCATGCCAAAT
CAGTTTTTCAAGCAAGGCTTTTAAACATCAAAAGAGCTGCACAGATTTAA
ATTTTACTACAGACATTATGAATTCGACTTCTCTTCCTAACAGTCCGAGT
AGAGATAATGTTAGCACAATAAATAATTTACTAAATTCACAAGATAATTC
GCTTTCGAATTCAATGGATACAATTGTCCACGAACCGGATGCTGCTGAAA
TTGAATTTTTGTCATCGAATTTAGATAATAGCGAAAGCGTTTCTTCAAAA
TCTCAGTCTAGCTTCAATACTATTTCATTAAACAATCCAAAACGAAAAAA
ATCTACCTTGTTTTCAGGAATCAACAATTATCGCAATAAAGTGATTAGTT
TTAAAAAGATTTTTAGTGACAGTGACGCTTCAAACGACCAATTATTGGGA
GACTATTCATGTGCACTAGCTAAAAGCACCCATGGCATTCTTATTCAAGG
CCGTCTCTATGTTACTGAAAAATGGATTTGTTTTTATTCGAAAATCATGT
CTTTTGAAAACAAAATTATTTTACCAATGTCGGGAATCAGCAACGTAAGT
AAAGAGAAAACCGTAAAAGTAATTCCAAATGCAATACAAATCACTACGAA
ATCCGGAAAATATTTTTTCACATCTTTCGGATCCCGTGAAAAATCATTCA
TAGTCCTTCAGACATTGTTAAACAAGCACAAAAATTGCGAAGCTTTGAAT
AATACTCAAAAGAAAAGTTTGATTCATGACTGTTACGGCAGTGAAGCTGA
TATTTATATTAGTGAGGAGTGCAATATCAATCAGAAAAATAACTTATCTG
AGAATGAAATCATGAAAAATTCCGAAGTACTTTTAAGAACCGAAGCGCCC
AGTTTTGTAGTTGGAGATAACTCAGATTCTGATACCGATCAGTCATTATA
TTCCAGTAATCTCAATCATCCTGATGAAATAGGCGACCATCTCCATCCAG
TCATAAAACATAGAGTTAAAAGGCATAAGTTCGATTCTCCCAATGCCGAA
TCATTATCCACTGAAGATAGCAATGTCAACTCAAATTTCAATTCTACAAG
CCTACCATCCACAAACGAATCAAATAGTATGTATAGGACATCTCTTTTGC
ACAACGTAGCTCAAATTTGCAATACTCATGAGCATTTCCATCGACTTTTT
GTGGACGAAGAAATTGACATGCACGTCGATCGATTGTTTTCATGTCTCTT
TACCAACAGCTCTTTTTTTCATCGGTTCACAGAATATCGAAAGACATTTG
ATATAATTCAGGCACCTTGGCCAGAACATGCTAACGAAAATGGAACCAAA
GAGCGAACGATTTACTATACAATTACTATGAAAGCTAAAATCGGACCAAA
AGTTTCGTCTTCCGAAGAAAAACAAATCATCCATGCAAATAGTGTACCGG
GTTGCTGCTATATCGTGGACACAGTTGTTCGCAATGGGGGTTTCCCATTG
TCTGATTGTTTTCATTGTATTAATAGATATTGTATTATTCATTTAAATGC
ATTTAAATCCAAACTAAAGGTCACTTCTCAAGTGGTTTATGACAAATCTA
TTTTTTTTGCTTCAAAATCTCTCATAGAATCGACAGCAAAATCTGGTCTC
ACCGATTATTTCAACGATCTCGCCAATTCATTAAAACATGAAGCCAAAGG
ATTAGGTCAGCATTTACGTCAGATAAATGTCAATCACGGTGCCACAAAAG
TCAGTTTACCAAAGCCAGTGCACCGTAAGATACCAAACGAGCCAACGCAT
CATCAGTCAAAAATTAATAAAACAATGAATGATTTGAATATGTCCCCGAT
GTCCGAAGACTTTACATTGTCAGTGGGAAATATTCTACGGCCGGACAGAG
CATGGTTGATACTAATAATGCTGAGTTTGATCATTTGTTTGTGTCTTTCA
TTTCTGTATTCAAGATTTCGACAGTTAGAAGAAATTACTAATATAGAAAT
TAATCGTCAAAATCATCCTTCCAGAGATCCATTGAGATTTGATTCATCTT
ACGACTGGAAAATAATGATGAAAAAACAACAACAAATTCAGATCGATGAA
ATATCTCAATGGAAAACACTTGTTGAATCTTCAATAGATTTAATTGATCA
GATGAGAAATGTCCTTGTCACTATTAAAGCCAACCTCGAAAATTTAGATG
ATGAAATCAAGTCGAATTCATTTCATTTCGACAACAAAGGCAAATATACC
AAATCATAATAATGTTGTAACACATGAAATTTGTAAAATGTTCACTTTTT
AAAGCCACCATTCCAGTCCAACTTTAATTTGGTGTTTGGTAGTTATTATT
CTACTTTCGGTTTATTAAAAGAACAAGTTATCACACTCATTAAAAAACCA
GCAATGATATTTATTTCCCTTTAAATTTATTTTTCTCAGTTTCAATAATT
TATTGTTTGACAGATTATCAG
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protein sequence of SMED30032364-orf-1

>SMED30032364-orf-1 ID=SMED30032364-orf-1|Name=SMED30032364-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=756bp
MADVFQCNSYSDNTQDPCQISFSSKAFKHQKSCTDLNFTTDIMNSTSLPN
SPSRDNVSTINNLLNSQDNSLSNSMDTIVHEPDAAEIEFLSSNLDNSESV
SSKSQSSFNTISLNNPKRKKSTLFSGINNYRNKVISFKKIFSDSDASNDQ
LLGDYSCALAKSTHGILIQGRLYVTEKWICFYSKIMSFENKIILPMSGIS
NVSKEKTVKVIPNAIQITTKSGKYFFTSFGSREKSFIVLQTLLNKHKNCE
ALNNTQKKSLIHDCYGSEADIYISEECNINQKNNLSENEIMKNSEVLLRT
EAPSFVVGDNSDSDTDQSLYSSNLNHPDEIGDHLHPVIKHRVKRHKFDSP
NAESLSTEDSNVNSNFNSTSLPSTNESNSMYRTSLLHNVAQICNTHEHFH
RLFVDEEIDMHVDRLFSCLFTNSSFFHRFTEYRKTFDIIQAPWPEHANEN
GTKERTIYYTITMKAKIGPKVSSSEEKQIIHANSVPGCCYIVDTVVRNGG
FPLSDCFHCINRYCIIHLNAFKSKLKVTSQVVYDKSIFFASKSLIESTAK
SGLTDYFNDLANSLKHEAKGLGQHLRQINVNHGATKVSLPKPVHRKIPNE
PTHHQSKINKTMNDLNMSPMSEDFTLSVGNILRPDRAWLILIMLSLIICL
CLSFLYSRFRQLEEITNIEINRQNHPSRDPLRFDSSYDWKIMMKKQQQIQ
IDEISQWKTLVESSIDLIDQMRNVLVTIKANLENLDDEIKSNSFHFDNKG
KYTKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR011993PH-like_dom_sf
IPR004182GRAM
IPR031968VASt
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 718..738
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 345..374
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 353..374
NoneNo IPR availablePANTHERPTHR23319UNCHARACTERIZEDcoord: 40..740
NoneNo IPR availableCDDcd13220PH-GRAM_GRAMDCcoord: 147..242
e-value: 2.17409E-38
score: 135.711
NoneNo IPR availableTMHMMTMhelixcoord: 637..656
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 135..206
e-value: 1.3E-12
score: 57.9
IPR004182GRAM domainPFAMPF02893GRAMcoord: 137..247
e-value: 4.2E-19
score: 68.5
IPR031968VASt domainPFAMPF16016VAStcoord: 404..552
e-value: 7.4E-29
score: 101.1
IPR031968VASt domainPROSITEPS51778VASTcoord: 398..572
score: 35.69
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 132..246
e-value: 1.6E-30
score: 107.2