SMED30032329

Overview


Name	SMED30032329
Smed ID	SMED30032329
Length (bp)	184

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032329

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30032329	SMESG000042387.1	Contig1419	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30032329	SMESG000042387.1	Contig1419	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30032329	SMESG000072323.1	Contig1419	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30032329	SMESG000072323.1	Contig1419	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
head region	SMED30032329	SMESG000042387.1	SmedASXL_006018	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
protonephridia	SMED30032329	SMESG000042387.1	dd_Smed_v4_6329_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30032329	SMESG000042387.1	dd_Smed_v4_6329_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30032329	SMESG000042387.1	dd_Smed_v4_6329_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032329	SMESG000042387.1	Contig1419	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032329	SMESG000042387.1	Contig1419	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032329	SMESG000072323.1	Contig1419	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30032329	SMESG000072323.1	Contig1419	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30032329	SMESG000042387.1	dd_Smed_v6_6329_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30032329 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001940:60693..63404 -	879

Homology

[Close]

BLAST of SMED30032329 vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 64.3142 bits (155), Expect = 1.535e-15
Identity = 28/42 (66.67%), Postives = 35/42 (83.33%), Query Frame = -1

Query:   50 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL 175
            ++LISTQ+R+  GPT VGDE+SDPELM +L A K +ALGNNL
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNL 44

[Close]

BLAST of SMED30032329 vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 63.1586 bits (152), Expect = 1.082e-14
Identity = 28/44 (63.64%), Postives = 35/44 (79.55%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE+SDPELM +L A K +ALGNN  E
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYE 46

[Close]

BLAST of SMED30032329 vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 43.1282 bits (100), Expect = 5.628e-7
Identity = 19/28 (67.86%), Postives = 22/28 (78.57%), Query Frame = -1

Query:   44 VGDEESDPELMTYLNAEKIKALGNNL*E 127
            VGDE+SDPELM +L A K +ALGNN  E
Sbjct:    2 VGDEQSDPELMQHLGASKRRALGNNFYE 29

[Close]

Query:   44 VGDEESDPELMTYLNAEKIKALGNNL*E 127
            VGDE+SDPELM +L A K +ALGNN  E
Sbjct:    2 VGDEQSDPELMQHLGASKRRALGNNFYE 29

[Close]

BLAST of SMED30032329 vs. Ensembl Celegans
Match: gfrp-1 (GTP cyclohydrolase I Feedback Regulatory Protein homolog [Source:UniProtKB/TrEMBL;Acc:D6RYD9])

HSP 1 Score: 52.7582 bits (125), Expect = 6.182e-11
Identity = 25/46 (54.35%), Postives = 32/46 (69.57%), Query Frame = -1

Query:   38 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*ELL 175
            ++LISTQ+R+  GPT VGD ESDP+LM  L A+  K LGN   E +
Sbjct:    3 YILISTQIRMECGPTMVGDGESDPKLMGTLEAKLTKQLGNEFQEYI 48

[Close]

BLAST of SMED30032329 vs. Ensembl Zebrafish
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:393735])

HSP 1 Score: 62.3882 bits (150), Expect = 1.502e-14
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+RL TGPT VGDE SDP +M YL A KI  LGNN  E
Sbjct:    3 YILISTQIRLETGPTMVGDEYSDPSIMNYLGARKITVLGNNFSE 46

[Close]

BLAST of SMED30032329 vs. Ensembl Xenopus
Match: eci2 (enoyl-CoA delta isomerase 2 [Source:Xenbase;Acc:XB-GENE-961246])

HSP 1 Score: 54.6842 bits (130), Expect = 2.200e-11
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            +VLISTQ+R+ TGPT VGDE SD +LM  L A+K   LGNN  E
Sbjct:    3 YVLISTQIRMETGPTIVGDEFSDTQLMAQLEADKRTVLGNNFSE 46

[Close]

BLAST of SMED30032329 vs. Ensembl Mouse
Match: Gchfr (GTP cyclohydrolase I feedback regulator [Source:MGI Symbol;Acc:MGI:2443977])

HSP 1 Score: 59.3066 bits (142), Expect = 2.568e-13
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE SDPELM +L A K   LGNN  E
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQHLGASKRSVLGNNFYE 46

[Close]

BLAST of SMED30032329 vs. UniProt/SwissProt
Match: sp|P30047|GFRP_HUMAN (GTP cyclohydrolase 1 feedback regulatory protein OS=Homo sapiens OX=9606 GN=GCHFR PE=1 SV=3)

HSP 1 Score: 63.1586 bits (152), Expect = 5.198e-14
Identity = 28/44 (63.64%), Postives = 35/44 (79.55%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE+SDPELM +L A K +ALGNN  E
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYE 46

[Close]

BLAST of SMED30032329 vs. UniProt/SwissProt
Match: sp|Q6PBT6|GFRP_DANRE (GTP cyclohydrolase 1 feedback regulatory protein OS=Danio rerio OX=7955 GN=gchfr PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.134e-13
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+RL TGPT VGDE SDP +M YL A KI  LGNN  E
Sbjct:    3 YILISTQIRLETGPTMVGDEYSDPSIMNYLGARKITVLGNNFSE 46

[Close]

BLAST of SMED30032329 vs. UniProt/SwissProt
Match: sp|Q32L41|GFRP_BOVIN (GTP cyclohydrolase 1 feedback regulatory protein OS=Bos taurus OX=9913 GN=GCHFR PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.558e-12
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE SDPELM +L A K   LGNN  E
Sbjct:    3 YLLISTQIRMEVGPTVVGDEHSDPELMQHLGASKRSVLGNNFSE 46

[Close]

BLAST of SMED30032329 vs. UniProt/SwissProt
Match: sp|P99025|GFRP_MOUSE (GTP cyclohydrolase 1 feedback regulatory protein OS=Mus musculus OX=10090 GN=Gchfr PE=1 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 1.796e-12
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE SDPELM +L A K   LGNN  E
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQHLGASKRSVLGNNFYE 46

[Close]

BLAST of SMED30032329 vs. UniProt/SwissProt
Match: sp|P70552|GFRP_RAT (GTP cyclohydrolase 1 feedback regulatory protein OS=Rattus norvegicus OX=10116 GN=Gchfr PE=1 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 2.310e-12
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+R+  GPT VGDE SDPELM  L A K + LGNN  E
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQQLGASKRRVLGNNFYE 46

[Close]

BLAST of SMED30032329 vs. TrEMBL
Match: A0A2J8QDM2 (GCHFR isoform 2 OS=Pan troglodytes OX=9598 GN=CK820_G0033505 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.185e-12
Identity = 28/42 (66.67%), Postives = 35/42 (83.33%), Query Frame = -1

Query:   50 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL 175
            ++LISTQ+R+  GPT VGDE+SDPELM +L A K +ALGNNL
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNL 44

[Close]

BLAST of SMED30032329 vs. TrEMBL
Match: H0YNM1 (GTP cyclohydrolase 1 feedback regulatory protein OS=Homo sapiens OX=9606 GN=GCHFR PE=1 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.185e-12
Identity = 28/42 (66.67%), Postives = 35/42 (83.33%), Query Frame = -1

Query:   50 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL 175
            ++LISTQ+R+  GPT VGDE+SDPELM +L A K +ALGNNL
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNL 44

[Close]

BLAST of SMED30032329 vs. TrEMBL
Match: A0A401P3Z4 (Uncharacterized protein OS=Scyliorhinus torazame OX=75743 GN=scyTo_0013745 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.771e-12
Identity = 32/44 (72.73%), Postives = 34/44 (77.27%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            +VLISTQ+RL TGPT VGDE SD ELM YL AEK  ALGNN  E
Sbjct:    3 YVLISTQIRLETGPTIVGDEHSDEELMRYLGAEKTTALGNNFSE 46

[Close]

BLAST of SMED30032329 vs. TrEMBL
Match: M1ERP8 (GTP cyclohydrolase I feedback regulator (Fragment) OS=Mustela putorius furo OX=9669 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.032e-12
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            +VLISTQ+R+  GPT VGDE SDPELM YL A K  ALGNN  E
Sbjct:    3 YVLISTQIRMEVGPTMVGDEHSDPELMQYLGASKRSALGNNFYE 46

[Close]

BLAST of SMED30032329 vs. TrEMBL
Match: M3YBF3 (GTP cyclohydrolase I feedback regulator OS=Mustela putorius furo OX=9669 GN=GCHFR PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.949e-12
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            +VLISTQ+R+  GPT VGDE SDPELM YL A K  ALGNN  E
Sbjct:    3 YVLISTQIRMEVGPTMVGDEHSDPELMQYLGASKRSALGNNFYE 46

[Close]

BLAST of SMED30032329 vs. Ensembl Nematostella
Match: EDO32067 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW52])

HSP 1 Score: 55.0694 bits (131), Expect = 3.542e-12
Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = -1

Query:   38 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*ELL 175
            ++LIST++RL  GPT  GDE SDPELM  L+AE +   GNN  E L
Sbjct:    3 YILISTRIRLELGPTQCGDEWSDPELMALLDAELVHQFGNNFKEYL 48

[Close]

BLAST of SMED30032329 vs. Ensembl Medaka
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:101171044])

HSP 1 Score: 58.9214 bits (141), Expect = 3.186e-13
Identity = 28/44 (63.64%), Postives = 32/44 (72.73%), Query Frame = -1

Query:   44 FVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 175
            ++LISTQ+RL TGPT VGDE SDP +M YL A K   LGNN  E
Sbjct:    3 YILISTQIRLETGPTNVGDEFSDPAVMNYLGARKTTVLGNNFSE 46

[Close]

BLAST of SMED30032329 vs. Ensembl Medaka
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:101171044])

HSP 1 Score: 44.669 bits (104), Expect = 1.242e-7
Identity = 21/33 (63.64%), Postives = 22/33 (66.67%), Query Frame = -1

Query:   44 TGPTYVGDEESDPELMTYLNAEKIKALGNNL*E 142
            TGPT VGDE SDP +M YL A K   LGNN  E
Sbjct:   27 TGPTNVGDEFSDPAVMNYLGARKTTVLGNNFSE 59

[Close]

BLAST of SMED30032329 vs. Planmine SMEST
Match: SMESG000042387.1 (SMESG000042387.1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.388e-24
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -1

Query:   50 MFVLISTQVRLVTGPTYVGDEESDPELMTYLNAEKIKALGNNL 178
            MFVLISTQVRL +GPTYVGDEESDPELMTYLNAEKIKALGNN 
Sbjct:    1 MFVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNF 43

The following BLAST results are available for this feature:

BLAST of SMED30032329 vs. Ensembl Human
BLAST of SMED30032329 vs. Ensembl Celegans
BLAST of SMED30032329 vs. Ensembl Fly
BLAST of SMED30032329 vs. Ensembl Zebrafish
BLAST of SMED30032329 vs. Ensembl Xenopus
BLAST of SMED30032329 vs. Ensembl Mouse
BLAST of SMED30032329 vs. UniProt/SwissProt
BLAST of SMED30032329 vs. TrEMBL
BLAST of SMED30032329 vs. Ensembl Cavefish
BLAST of SMED30032329 vs. Ensembl Sea Lamprey
BLAST of SMED30032329 vs. Ensembl Yeast
BLAST of SMED30032329 vs. Ensembl Nematostella
BLAST of SMED30032329 vs. Ensembl Medaka
BLAST of SMED30032329 vs. Planmine SMEST

BLAST of SMED30032329 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
GCHFR	1.535e-15	66.67	GTP cyclohydrolase I feedback regulator [Source:HG...	[more]
GCHFR	1.082e-14	63.64	GTP cyclohydrolase I feedback regulator [Source:HG...	[more]
GCHFR	5.628e-7	67.86	GTP cyclohydrolase I feedback regulator [Source:HG...	[more]
GCHFR	5.628e-7	67.86	GTP cyclohydrolase I feedback regulator [Source:HG...	[more]

BLAST of SMED30032329 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gfrp-1	6.182e-11	54.35	GTP cyclohydrolase I Feedback Regulatory Protein h...	[more]

BLAST of SMED30032329 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032329 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gchfr	1.502e-14	65.91	GTP cyclohydrolase I feedback regulator [Source:NC...	[more]

BLAST of SMED30032329 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
eci2	2.200e-11	61.36	enoyl-CoA delta isomerase 2 [Source:Xenbase;Acc:XB...	[more]

BLAST of SMED30032329 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Gchfr	2.568e-13	61.36	GTP cyclohydrolase I feedback regulator [Source:MG...	[more]

BLAST of SMED30032329 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P30047\|GFRP_HUMAN	5.198e-14	63.64	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]
sp\|Q6PBT6\|GFRP_DANRE	1.134e-13	65.91	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]
sp\|Q32L41\|GFRP_BOVIN	1.558e-12	61.36	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]
sp\|P99025\|GFRP_MOUSE	1.796e-12	61.36	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]
sp\|P70552\|GFRP_RAT	2.310e-12	61.36	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]

BLAST of SMED30032329 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2J8QDM2	2.185e-12	66.67	GCHFR isoform 2 OS=Pan troglodytes OX=9598 GN=CK82...	[more]
H0YNM1	2.185e-12	66.67	GTP cyclohydrolase 1 feedback regulatory protein O...	[more]
A0A401P3Z4	2.771e-12	72.73	Uncharacterized protein OS=Scyliorhinus torazame O...	[more]
M1ERP8	5.032e-12	68.18	GTP cyclohydrolase I feedback regulator (Fragment)...	[more]
M3YBF3	5.949e-12	68.18	GTP cyclohydrolase I feedback regulator OS=Mustela...	[more]

BLAST of SMED30032329 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032329 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032329 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032329 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO32067	3.542e-12	56.52	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30032329 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gchfr	3.186e-13	63.64	GTP cyclohydrolase I feedback regulator [Source:NC...	[more]
gchfr	1.242e-7	63.64	GTP cyclohydrolase I feedback regulator [Source:NC...	[more]

BLAST of SMED30032329 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000042387.1	5.388e-24	93.02	SMESG000042387.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30032329 ID=SMED30032329|Name=SMED30032329|organism=Schmidtea mediterranea sexual|type=transcript|length=184bp
CAAGTTTCGAGCTATACGTAAAATTATATGTAAATAATAATAATTCTTAC
AAATTATTTCCTAAAGCTTTAATTTTTTCTGCATTTAGATATGTCATCAA
TTCCGGGTCGCTTTCCTCGTCTCCTACATATGTTGGGCCAGTTACTAATC
TCACTTGCGTACTAATTAGAACAAACATGCATAT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000070	intestinal phagocyte
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR036717	GFRP_sf
IPR009112	GTP_CycHdrlase_I_reg

Vocabulary: **biological process**
Term	Definition
GO:0009890	negative regulation of biosynthetic process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009112	GTP cyclohydrolase I, feedback regulatory protein	PFAM	PF06399	GFRP	coord: 4..44 e-value: 3.6E-15 score: 55.6
IPR036717	GFRP superfamily	GENE3D	G3DSA:3.30.1410.10		coord: 3..45 e-value: 5.3E-12 score: 47.3
IPR036717	GFRP superfamily	SUPERFAMILY	SSF69761	GTP cyclohydrolase I feedback regulatory protein, GFRP	coord: 4..44
None	No IPR available	PRODOM	PD028559		coord: 10..45 e-value: 3.0E-8 score: 133.0

Main menu

SMED30032329

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

blazegraph-runner