SMED30032324

Overview


Name	SMED30032324
Smed ID	SMED30032324
Length (bp)	1645

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30032324 (SMED30032324) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032324 (SMED30032324) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032324 (SMED30032324) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032324 (SMED30032324) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032324

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30032324	SMESG000079516.1	dd_Smed_v4_8794_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30032324	SMESG000079516.1	dd_Smed_v4_8794_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30032324	SMESG000079516.1	dd_Smed_v4_8794_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30032324	SMESG000079516.1	dd_Smed_v4_8794_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30032324	SMESG000079516.1	dd_Smed_v4_8794_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30032324 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.002203:3797..5441 -	8135

Homology

[Close]

BLAST of SMED30032324 vs. TrEMBL
Match: G4V7W3 (Putative monocarboxylate transporter OS=Schistosoma mansoni OX=6183 GN=Smp_079640 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.507e-23
Identity = 114/460 (24.78%), Postives = 193/460 (41.96%), Query Frame = 1

Query:  178 FNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIE-MNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSI--GFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFI 1515
            F++I+ E       +  W+   RL    +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I  +   YR  + ++GL+  I+C+T++ V  P +  LT + I G GS +      L  ++ F + +RF V ATA   G   G L    L++ L +  GW +  QL         CLAG+  I   +         +    ++   +K L   SL L     P+ +  +      +E +  +P VF W   R    ++   ++ L++  +  ++    + L  Q        L +  GES+ + LGA  G  I GYL +  +       LFLV +      L +         V A  TG  +G   T L A SEE  M+ G   FP  K+I G+    +P     +        + ++ ++L +  A LL FI
Sbjct:   15 FHTIYVEFSRARRKLYGWR---RLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRWNRPYRCTMVLAGLLIFISCMTSAVVPSPGYLFLTHTLIHGLGSTLILCGTSLVTEEYFDRSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQSYGWQVTFQLSGLLFLFVTCLAGVVFISRDIFEYNQAMLEISDTFEENTMNKALQKDSLLL--LNSPKASDGRGWAFCSVEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVS----EALNDQLVLGARVNLYFGIGESVVFTLGALIGDRIRGYLAYAYLIGALFAALFLVIMQNTYEDLNVVY-------VLAGFTGATVGVGNTFLYATSEEIMMVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAILVSIRAILLGFI 454

[Close]

BLAST of SMED30032324 vs. TrEMBL
Match: A0A183A5S6 (MFS domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2311 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.649e-23
Identity = 119/453 (26.27%), Postives = 185/453 (40.84%), Query Frame = 1

Query:  262 IFACALS-ITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILT-CPEHGLKVR--VKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNY---SPSVFPWLVYRFFMTIIHQGV------FTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL-LHVSIG-FLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVF---ILHRLYTKYLENSKDSKM 1566
            +F  AL+ I +  K R   +   AL   +N     +++ WIG  YA +   ++P  T +I      YRF + ++G+   ++C+T++TV  P +  LT + + G GS +      L   D F K +RF V ATA   G   G L    LF++  +H  W    +    LF     +  +T  P H +  +  V +     G  +    L     P  +  +  G  F  M +   +P VF W   RF   I+  G+      +     D+ LI    ++             L +  GESL + +GA  G  I GYL L   IG     LFL+ V      + +         V A  TG  +G   T L A +EE  ++ G   FP  K+I G+    +P L   +        + ++ +VL      LL     ILHR   K++      KM
Sbjct:   40 LFVSALNVICIGGKRRAFGIFVAALHTAYNET-SMSELNWIGDSYASLGFFLMPFATTIIVQSNRPYRFTMFLAGVTIFVSCMTSATVPDPGYLFLTHTLLHGIGSTLVLCGTSLVTGDYFDKTHRFHVLATAFVSGGPYGVLVFGPLFSHWIQHYSWQQAFIYSGILFLFVTWLGAITFLPRHMVDYQQAVTENQNKTGEQNSQAPLLLRNAPRASDGR--GWAFCSMAHLRANPQVFLWGFERFLHNIVIYGLLMNLTAYVSQALDNELIRGAKVN-------------LYFGIGESLIFTIGALIGDRIRGYLPLTYLIGAAFAALFLLIVQKSYTDINVVY-------VLAGFTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMIAGIGMLVAPALSGSIIDGYGYRGFFISMAVLVCLRVALLTLICIILHRKKQKFMLEEMQRKM 469

[Close]

BLAST of SMED30032324 vs. TrEMBL
Match: A0A183REE3 (MFS domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.838e-23
Identity = 105/424 (24.76%), Postives = 178/424 (41.98%), Query Frame = 1

Query:  283 ITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWM----------MLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIE-MNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSI--GFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFI 1515
            + +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I  +   YR  + ++GL+  I+C+T++ V  P +  LT + I G GS +      L  ++ F + +RF V ATA   G   G L    L++ L +  GW           +L  CLAG+  I   +         +    ++   +K L   SL L +   P+ +  +      +E +  +P VF W   R    ++   ++ L++  +  ++    + L  Q        L +  GES+ + LGA  G  I GYL +  +       LFLV +      L +         V A  TG  +G   T L A SEE  M+ G   FP  K+I G+    +P     +        + ++ ++L +  A LL FI
Sbjct:   48 VCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRWNRPYRCTMVLAGLLIFISCMTSAVVPSPGYLFLTHTLIHGLGSTLILCGTSLVTEEYFDRSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQSYGWQVTFQLSGLLFLLVTCLAGVVFISRDIFEYNQAMLEISDTFEENTMNKALRKDSLLLHN--SPKASDGRRWAFCSVEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVS----EALNDQLVMGARVNLYFGIGESVVFTLGALIGDRIRGYLAYAYLIGALFAALFLVIMQNTYEDLNVVY-------VLAGFTGATVGVGNTFLYATSEEIMMVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAILVSIRAILLGFI 454

[Close]

BLAST of SMED30032324 vs. TrEMBL
Match: C1LE48 (Major facilitator superfamily OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.234e-22
Identity = 116/492 (23.58%), Postives = 205/492 (41.67%), Query Frame = 1

Query:  199 EKVFNTDVVNWQIRVRLVLFS--------IFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESF---QKPEITPNKLLGL-TFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL---LHVSIGFLCILFLV-TVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLL---VFILHRLYTKYLENSKDSKMTIQVSM 1584
            + +F+T  V++  R RL L+         +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I      YRF + ++G++  ++C+T++ V  P +  LT + I G GS +      L   + F K +RF V ATA   G   G L    L++ L ++ GW +  QL         CLAG+  I   M      E+   +        +   DKSL  +S      P+ +  +     +F  +  +P VF W   R    ++   ++ L++  +  ++      L  Q        L +  GES+ + +GA  G  I GYL     +   F  +  L+   T++ + +           V A   G  +G   T L A SEE  ++ G   FP  K+I G+    +P     +        + ++ +VL +    LL    +ILHR     ++  K+   T + ++
Sbjct:   12 QSIFHTIYVHFS-RARLKLYGWRHLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRLNRPYRFTMLLAGILIFVSCMTSAAVPSPGYLFLTHTLIHGIGSTLILCGTSLVTGEYFDKSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQNYGWQVAFQLSGLLFLFVTCLAGIVFISRDMF-----EYHQAISETFEASEENTLDKSLRRDSLLLRNSPKASDGRGWAFCSFEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVSQA----LNDQLVLGARVNLYFGIGESVVFTVGALIGDRIRGYLAFAYFIGASFAALFLLIMQNTYEDINVVY---------VLAGFAGATVGVGNTFLYATSEEIMLVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAVLVSIRVILLGVICYILHRKKKLIIDTEKEHASTNENNL 480

[Close]

BLAST of SMED30032324 vs. TrEMBL
Match: A0A4Z2DS50 (Major facilitator superfamily OS=Schistosoma japonicum OX=6182 GN=EWB00_009543 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.403e-22
Identity = 116/492 (23.58%), Postives = 205/492 (41.67%), Query Frame = 1

Query:  199 EKVFNTDVVNWQIRVRLVLFS--------IFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESF---QKPEITPNKLLGL-TFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL---LHVSIGFLCILFLV-TVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLL---VFILHRLYTKYLENSKDSKMTIQVSM 1584
            + +F+T  V++  R RL L+         +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I      YRF + ++G++  ++C+T++ V  P +  LT + I G GS +      L   + F K +RF V ATA   G   G L    L++ L ++ GW +  QL         CLAG+  I   M      E+   +        +   DKSL  +S      P+ +  +     +F  +  +P VF W   R    ++   ++ L++  +  ++      L  Q        L +  GES+ + +GA  G  I GYL     +   F  +  L+   T++ + +           V A   G  +G   T L A SEE  ++ G   FP  K+I G+    +P     +        + ++ +VL +    LL    +ILHR     ++  K+   T + ++
Sbjct:   12 QSIFHTIYVHFS-RARLKLYGWRHLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRLNRPYRFTMLLAGILIFVSCMTSAAVPSPGYLFLTHTLIHGIGSTLILCGTSLVTGEYFDKSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQNYGWQVAFQLSGLLFLFVTCLAGIVFISRDMF-----EYHQAISETFEASEENTLDKSLRRDSLLLRNSPKASDGRGWAFCSFEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVSQA----LNDQLVLGARVNLYFGIGESVVFTVGALIGDRIRGYLAFAYFIGASFAALFLLIMQNTYEDINVVY---------VLAGFAGATVGVGNTFLYATSEEIMLVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAVLVSIRVILLGVICYILHRKKKLIIDTEKEHASTNENNL 480

[Close]

BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000079516.1 (SMESG000079516.1)

HSP 1 Score: 997.268 bits (2577), Expect = 0.000e+0
Identity = 500/501 (99.80%), Postives = 501/501 (100.00%), Query Frame = 1

Query:  118 MQDENEIPXXXXXXXXXXXXFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS 1620
            MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDIL+PQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS
Sbjct:    1 MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILLPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS 501

[Close]

BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026518.1 (SMESG000026518.1)

HSP 1 Score: 83.9593 bits (206), Expect = 4.936e-17
Identity = 87/374 (23.26%), Postives = 151/374 (40.37%), Query Frame = 1

Query:  340 HFNNKFPQ---AQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVK---QRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFI-------LGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFL 1422
             FN ++P     ++ WIG  Y+        +   +I Y+   Y     +  L  +I+CIT+S V +P +  LT +   G GS++  +   L V++ F K +R+ + AT L  G ++ S+  + LF++L +   W        G   I   +  + C    +  R     +   S G S  S +                 T+I     P +  W V R   +++  G+   + T S+      +D        +++S +L+ASGE+  YL+GA             L Y+L V+   +C   ++   ++  +  +   + V         G  +G   T L  A EE T +PG   +PF     G     SP L
Sbjct:   44 QFNKEYPNISLTELNWIGDSYSAFGYMSTSLTVTIIIYFNRRYGLFQFLGALWVLISCITSSFVPNPHWLFLTHTLFHGIGSSLIISTAGLVVNEYFDKKHRYHILATTLVSGGSVASILFVMLFSFLIQKYTW-------EGALQILGVLYFIVCGCAAIFFRKNPDLKEVSSTGKSKYSCQ-----------------TYIRWKNIPLLILWFVDRVLTSVVTYGLLLNLTTHSYEKHYSLVD--------SSISTVLFASGEASTYLIGAIIMAITKDLFKERLRYVLLVTSFTMCASLILIYFFEANKTVLRSLLFVA--------GFSLGPSITFLFPAGEELTRLPGHVAYPFSLGGMGFGMMISPLL 377

[Close]

BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026692.1 (SMESG000026692.1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.942e-17
Identity = 96/385 (24.94%), Postives = 167/385 (43.38%), Query Frame = 1

Query:  349 NKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKS-----LELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGA-------AFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLL 1467
            N    A++ WIG  YA +      + T +I      YR    I   + ++AC+T++ V +  +  LT + + GFGS++  +   L V++ F K +   + AT L  G ++ S+  +  +AYL    GW    + L  ++ +   + +  C     K   K     +GLS KS      +++S Q P +                  +F W + R   +I+  G+  L L D    +   +        +T+L+ +L+A+GE+  YL+G         F +  L Y+L VS   + +     V W+ V      S ++     A ++G C+G   T L  A EE T +PG   +PF     G+    SP L  ++ +I + N + L
Sbjct:   51 NSTSLAELNWIGDSYAALGYISTTLSTALILRSNRRYRLFQFIGAFLILLACLTSAVVPNAHWLFLTHTVLHGFGSSLILSTVGLIVNEYFDKNHPRHILATTLVSGGSVASIVFVQFYAYLIVDYGWRKAFVILGLIYFV---VNLSACFVFARKFSNKSE---EGLSKKSSVNVCRDIKSSQIPLL------------------IF-WTIDRIMTSIVTYGML-LNLADYMRRSESKLT------NSTSLT-VLFAAGEASTYLIGTIITGLTKKFFHNRLKYILLVSTFLMAVSL---VYWEFVSSNKHLSRLL-----AFVSGFCLGPSITFLFPAGEEMTTLPGHMAYPFSLFGMGIGMAVSPILTALIAQIFDYNWFFL 394

[Close]

BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026516.1 (SMESG000026516.1)

HSP 1 Score: 77.7962 bits (190), Expect = 5.174e-15
Identity = 85/394 (21.57%), Postives = 165/394 (41.88%), Query Frame = 1

Query:  340 HFNNKFPQ---AQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVF 1512
             F+ ++P     ++ WIG  Y+ I      +   +I Y+   Y     +  L  +I+CIT+S V +P +  LT +   G GS++  +   L V++ F K +R+ + AT L  G ++ S+  + LF+ L +H GW    + L  ++ +  ++      ++     +  +G  K L      ++S Q P I                  +F W   R   +++  G+  L LTD       D+         +++  +++ASGE++ YL+G+     I        + ++ ++  ++++   + L+    ++    +     G  +G   T L  A EE T +PG   +P      G     SP L       + + I LL +        C ++F
Sbjct:   45 QFHKEYPNISLTELNWIGDSYSAIGYMSTSLSISIIIYFNRRYGLFQFLGALWVLISCITSSFVPNPHWLFLTHTLFHGIGSSLIISTAGLVVNEYFDKKHRYHILATTLVSGGSVASILFVTLFSKLIEHYGWRKTFIILGIIYFVVCSLAAFFFKKNKDLKEISSKG--KMLCQGQWNIKSIQIPLI------------------IF-WFTDRIMTSVVTYGLL-LNLTDYVYQGHKDL-------MKSSILTIIFASGEAVTYLIGSVITA-ITKDFFKEKLRYILLVTSLSMSICLIILEYTSHIVSIRRLLCFFAGFSLGPSITFLFPAGEEFTRLPGHLAYPVSLGGMGFGMIISPLL-------TAKIIELLDYRWFFFVQGCFILF 401

[Close]

BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026519.1 (SMESG000026519.1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.349e-14
Identity = 88/364 (24.18%), Postives = 149/364 (40.93%), Query Frame = 1

Query:  367 QMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAA------------FGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFL 1422
            ++ WIG  YA +      I   +I ++K  Y     +  +  +++C+T+S V +P +  LT +   G GS++  +   L V+D F   + + + AT L  G ++ S+  + LFA L +  GW    + L  L+ I  A   L   +   +V    +        K++        +I    LL L             W + R   +++  G+  L LTD    T  ++        +TTL+  L+ASGE++ YL+GA               Y +LG    +SI   C+          + L+  + ++    +   L G  +G   T L  A EE T +PG   +P      G     SP L
Sbjct:   56 ELNWIGDSYAAVGYMTSGISIAIILFFKRRYGMFQFMGAIWVLVSCVTSSFVPNPHWLFLTHTLFHGIGSSLIISTVGLVVNDHFDSKHPYHILATTLVSGGSVASIIFVTLFASLIQSYGWRYAFIILGCLYFIVNASAALFFRKSNNQVEDTNK--------KTVSCRCEGHIKIIHIPLLCL-------------WFIDRVMTSVVTYGLL-LNLTDYMYRTEKNL------VRSTTLTT-LFASGEAITYLIGATITAITRDFFKDKLRYILLGTSFAMSI---CL----------IALEYFKGIISVCYILCFLAGFTLGPSITFLFPAGEELTRLPGHMAYPISLAGMGFGMLVSPLL 377

The following BLAST results are available for this feature:

BLAST of SMED30032324 vs. Ensembl Human
BLAST of SMED30032324 vs. Ensembl Celegans
BLAST of SMED30032324 vs. Ensembl Fly
BLAST of SMED30032324 vs. Ensembl Zebrafish
BLAST of SMED30032324 vs. Ensembl Xenopus
BLAST of SMED30032324 vs. Ensembl Mouse
BLAST of SMED30032324 vs. UniProt/SwissProt
BLAST of SMED30032324 vs. TrEMBL
BLAST of SMED30032324 vs. Ensembl Cavefish
BLAST of SMED30032324 vs. Ensembl Sea Lamprey
BLAST of SMED30032324 vs. Ensembl Yeast
BLAST of SMED30032324 vs. Ensembl Nematostella
BLAST of SMED30032324 vs. Ensembl Medaka
BLAST of SMED30032324 vs. Planmine SMEST

BLAST of SMED30032324 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
G4V7W3	3.507e-23	24.78	Putative monocarboxylate transporter OS=Schistosom...	[more]
A0A183A5S6	3.649e-23	26.27	MFS domain-containing protein OS=Echinostoma capro...	[more]
A0A183REE3	4.838e-23	24.76	MFS domain-containing protein OS=Schistosoma rodha...	[more]
C1LE48	1.234e-22	23.58	Major facilitator superfamily OS=Schistosoma japon...	[more]
A0A4Z2DS50	1.403e-22	23.58	Major facilitator superfamily OS=Schistosoma japon...	[more]

BLAST of SMED30032324 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30032324 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000079516.1	0.000e+0	99.80	SMESG000079516.1	[more]
SMESG000026518.1	4.936e-17	23.26	SMESG000026518.1	[more]
SMESG000026692.1	9.942e-17	24.94	SMESG000026692.1	[more]
SMESG000026516.1	5.174e-15	21.57	SMESG000026516.1	[more]
SMESG000026519.1	8.349e-14	24.18	SMESG000026519.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30032324 ID=SMED30032324|Name=SMED30032324|organism=Schmidtea mediterranea sexual|type=transcript|length=1645bp
GCAGATTGGTTTGCTATGTTCACATTTTGTTTTAATTGTTATTTACCGAA
AGTGTGTTACTCACAATACTTTTGTCAGCCTACACCAAAAACAGAATTTT
AAATCGATAAAATAGAAATGCAAGATGAAAATGAAATTCCGAAACGAAAA
TTGAGTTTGAAGAAATCAAGAAAGAAGTTCAATTCAATACACGAAGAGGA
GAAAGTGTTCAACACGGATGTCGTCAACTGGCAGATCCGCGTCAGATTGG
TCTTGTTCTCAATATTTGCATGTGCGCTATCAATCACGGTCGCAGCTAAG
ATCCGATTTACCCCGGTCATTTCCCATGCACTTTCTGTGCATTTTAACAA
CAAATTCCCTCAAGCTCAAATGGAATGGATTGGGAGGTTATACGCGATTA
TTGTGCTCTGTATGCTGCCAATAGTTACTGGAGTAATAGATTATTATAAA
ATCGGCTATCGGTTTGCACTCTGTATATCGGGACTCATGCACATGATTGC
TTGTATTACAAACTCAACTGTTGTCCATCCATTGTTTTTTGTTTTAACTG
ACTCTTTCATATGCGGCTTTGGATCAGCTATATCATTTGCGGTTTTCCTC
CTAGCTGTCGATGACATATTTCCAAAAGGAAACCGGTTCAGAGTTCCTGC
AACGGCCCTTTGTCAGGGTTCTACAATTGGATCATTATTCATTTTGGACC
TTTTCGCTTATCTTGCGAAACATTTGGGTTGGATGATGTTACAGTTGTGT
TTAGCGGGCCTATTTTCGATCGAAGCCGCCATGTTAATATTAACATGTCC
AGAACACGGGTTGAAAGTTCGTGTGAAACAAAGAGGAGGTAGTAAAGGTC
TAAGTGACAAATCGCTTGAATTGGAAAGTTTCCAAAAACCGGAAATAACT
CCTAATAAATTATTAGGCTTAACATTTATAGAAATGAATTACAGTCCATC
AGTGTTTCCTTGGCTAGTCTACAGATTCTTTATGACGATCATTCATCAAG
GAGTTTTTACTCTCGTTTTAACCGACAGTCACCTCATCACAACGCCAGAC
ATTGACATTCTGATGCCTCAGTATTACGCAACCACTTTGAGCGCCTTGTT
ATATGCTTCAGGCGAATCATTATTTTATCTTCTCGGAGCTGCATTTGGAT
ATTTTATTCTTGGTTACCTTTTGCATGTAAGCATTGGGTTTTTGTGCATC
TTATTTCTTGTAACTGTCACGTGGCAACCGGTGAGACTGCAAATGGCTCA
GTCAATGATGGTTGGAAATGCAGTCTGTGCTTGCTTGACTGGTGTTTGCA
TTGGAGCGTTGGGAACCTGCCTCATCGCTGCTTCTGAGGAGGCAACAATG
ATTCCTGGGAGAAAAATCTTTCCTTTCGTCAAATTAATCGAAGGATTAGC
ATTTACGTTTAGTCCTTTTCTTTTTGACATTTTAAACAAAATCTCTGAAC
AAAACATTTGGTTGTTGACGTTTTCTGTCCTAACAGCAGCAAATGCTTGC
TTATTAGTATTTATTCTGCATAGGTTATACACAAAGTATCTGGAAAACTC
TAAGGACAGTAAGATGACAATACAAGTCTCAATGAGGCCGGTAACACTAT
TATCCAAATTTGATTCTTCATAACTTATAGCTATGTATTTAATCC

protein sequence of SMED30032324-orf-1

>SMED30032324-orf-1 ID=SMED30032324-orf-1|Name=SMED30032324-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
IKYIAISYEESNLDNSVTGLIETCIVILLSLEFSRYFVYNLCRINTNKQA
FAAVRTENVNNQMFCSEILFKMSKRKGLNVNANPSINLTKGKIFLPGIIV
ASSEAAMRQVPNAPMQTPVKQAQTAFPTIID*

protein sequence of SMED30032324-orf-2

>SMED30032324-orf-2 ID=SMED30032324-orf-2|Name=SMED30032324-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=502bp
MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIF
ACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCML
PIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICG
FGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLA
KHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSL
ELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLV
LTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGY
LLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGT
CLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLL
TFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDS
S*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036259	MFS_trans_sf
IPR011701	MFS
IPR020846	MFS_dom

Vocabulary: **molecular function**
Term	Definition
GO:0022857	transmembrane transporter activity

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane
GO:0016020	membrane

Vocabulary: **biological process**
Term	Definition
GO:0055085	transmembrane transport

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 49..407 e-value: 1.2E-8 score: 34.4
None	No IPR available	GENE3D	G3DSA:1.20.1250.20		coord: 35..236 e-value: 4.5E-7 score: 31.4
None	No IPR available	CDD	cd17352	MFS_MCT_SLC16	coord: 44..216 e-value: 1.61621E-4 score: 41.7693
None	No IPR available	TMHMM	TMhelix		coord: 45..67
None	No IPR available	TMHMM	TMhelix		coord: 114..136
None	No IPR available	TMHMM	TMhelix		coord: 279..301
None	No IPR available	TMHMM	TMhelix		coord: 203..225
None	No IPR available	TMHMM	TMhelix		coord: 382..404
None	No IPR available	TMHMM	TMhelix		coord: 449..471
None	No IPR available	TMHMM	TMhelix		coord: 417..439
None	No IPR available	TMHMM	TMhelix		coord: 345..367
None	No IPR available	TMHMM	TMhelix		coord: 176..198
None	No IPR available	TMHMM	TMhelix		coord: 87..107
None	No IPR available	TMHMM	TMhelix		coord: 141..163
None	No IPR available	TMHMM	TMhelix		coord: 316..338
IPR036259	MFS transporter superfamily	SUPERFAMILY	SSF103473	MFS general substrate transporter	coord: 36..469

Main menu

SMED30032324

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

blazegraph-runner