SMED30032324

Overview
NameSMED30032324
Smed IDSMED30032324
Length (bp)1645
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032324 (SMED30032324) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032324 (SMED30032324) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032324 (SMED30032324) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032324 (SMED30032324) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032324

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30032324h1SMcG0009371 dd_Smed_v4_8794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30032324h1SMcG0009371 dd_Smed_v4_8794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032324h1SMcG0009371 dd_Smed_v4_8794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30032324h1SMcG0009371 dd_Smed_v4_8794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30032324h1SMcG0009371 dd_Smed_v4_8794_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032324 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002203:3797..5441 -8135
Homology
BLAST of SMED30032324 vs. TrEMBL
Match: G4V7W3 (Putative monocarboxylate transporter OS=Schistosoma mansoni OX=6183 GN=Smp_079640 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.507e-23
Identity = 114/460 (24.78%), Postives = 193/460 (41.96%), Query Frame = 1
Query:  178 FNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIE-MNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSI--GFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFI 1515
            F++I+ E       +  W+   RL    +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I  +   YR  + ++GL+  I+C+T++ V  P +  LT + I G GS +      L  ++ F + +RF V ATA   G   G L    L++ L +  GW +  QL         CLAG+  I   +         +    ++   +K L   SL L     P+ +  +      +E +  +P VF W   R    ++   ++ L++  +  ++    + L  Q        L +  GES+ + LGA  G  I GYL +  +       LFLV +      L +         V A  TG  +G   T L A SEE  M+ G   FP  K+I G+    +P     +        + ++ ++L +  A LL FI
Sbjct:   15 FHTIYVEFSRARRKLYGWR---RLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRWNRPYRCTMVLAGLLIFISCMTSAVVPSPGYLFLTHTLIHGLGSTLILCGTSLVTEEYFDRSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQSYGWQVTFQLSGLLFLFVTCLAGVVFISRDIFEYNQAMLEISDTFEENTMNKALQKDSLLL--LNSPKASDGRGWAFCSVEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVS----EALNDQLVLGARVNLYFGIGESVVFTLGALIGDRIRGYLAYAYLIGALFAALFLVIMQNTYEDLNVVY-------VLAGFTGATVGVGNTFLYATSEEIMMVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAILVSIRAILLGFI 454          
BLAST of SMED30032324 vs. TrEMBL
Match: A0A183A5S6 (MFS domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2311 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.649e-23
Identity = 119/453 (26.27%), Postives = 185/453 (40.84%), Query Frame = 1
Query:  262 IFACALS-ITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILT-CPEHGLKVR--VKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNY---SPSVFPWLVYRFFMTIIHQGV------FTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL-LHVSIG-FLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVF---ILHRLYTKYLENSKDSKM 1566
            +F  AL+ I +  K R   +   AL   +N     +++ WIG  YA +   ++P  T +I      YRF + ++G+   ++C+T++TV  P +  LT + + G GS +      L   D F K +RF V ATA   G   G L    LF++  +H  W    +    LF     +  +T  P H +  +  V +     G  +    L     P  +  +  G  F  M +   +P VF W   RF   I+  G+      +     D+ LI    ++             L +  GESL + +GA  G  I GYL L   IG     LFL+ V      + +         V A  TG  +G   T L A +EE  ++ G   FP  K+I G+    +P L   +        + ++ +VL      LL     ILHR   K++      KM
Sbjct:   40 LFVSALNVICIGGKRRAFGIFVAALHTAYNET-SMSELNWIGDSYASLGFFLMPFATTIIVQSNRPYRFTMFLAGVTIFVSCMTSATVPDPGYLFLTHTLLHGIGSTLVLCGTSLVTGDYFDKTHRFHVLATAFVSGGPYGVLVFGPLFSHWIQHYSWQQAFIYSGILFLFVTWLGAITFLPRHMVDYQQAVTENQNKTGEQNSQAPLLLRNAPRASDGR--GWAFCSMAHLRANPQVFLWGFERFLHNIVIYGLLMNLTAYVSQALDNELIRGAKVN-------------LYFGIGESLIFTIGALIGDRIRGYLPLTYLIGAAFAALFLLIVQKSYTDINVVY-------VLAGFTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMIAGIGMLVAPALSGSIIDGYGYRGFFISMAVLVCLRVALLTLICIILHRKKQKFMLEEMQRKM 469          
BLAST of SMED30032324 vs. TrEMBL
Match: A0A183REE3 (MFS domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.838e-23
Identity = 105/424 (24.76%), Postives = 178/424 (41.98%), Query Frame = 1
Query:  283 ITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWM----------MLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIE-MNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSI--GFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFI 1515
            + +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I  +   YR  + ++GL+  I+C+T++ V  P +  LT + I G GS +      L  ++ F + +RF V ATA   G   G L    L++ L +  GW           +L  CLAG+  I   +         +    ++   +K L   SL L +   P+ +  +      +E +  +P VF W   R    ++   ++ L++  +  ++    + L  Q        L +  GES+ + LGA  G  I GYL +  +       LFLV +      L +         V A  TG  +G   T L A SEE  M+ G   FP  K+I G+    +P     +        + ++ ++L +  A LL FI
Sbjct:   48 VCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRWNRPYRCTMVLAGLLIFISCMTSAVVPSPGYLFLTHTLIHGLGSTLILCGTSLVTEEYFDRSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQSYGWQVTFQLSGLLFLLVTCLAGVVFISRDIFEYNQAMLEISDTFEENTMNKALRKDSLLLHN--SPKASDGRRWAFCSVEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVS----EALNDQLVMGARVNLYFGIGESVVFTLGALIGDRIRGYLAYAYLIGALFAALFLVIMQNTYEDLNVVY-------VLAGFTGATVGVGNTFLYATSEEIMMVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAILVSIRAILLGFI 454          
BLAST of SMED30032324 vs. TrEMBL
Match: C1LE48 (Major facilitator superfamily OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.234e-22
Identity = 116/492 (23.58%), Postives = 205/492 (41.67%), Query Frame = 1
Query:  199 EKVFNTDVVNWQIRVRLVLFS--------IFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESF---QKPEITPNKLLGL-TFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL---LHVSIGFLCILFLV-TVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLL---VFILHRLYTKYLENSKDSKMTIQVSM 1584
            + +F+T  V++  R RL L+         +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I      YRF + ++G++  ++C+T++ V  P +  LT + I G GS +      L   + F K +RF V ATA   G   G L    L++ L ++ GW +  QL         CLAG+  I   M      E+   +        +   DKSL  +S      P+ +  +     +F  +  +P VF W   R    ++   ++ L++  +  ++      L  Q        L +  GES+ + +GA  G  I GYL     +   F  +  L+   T++ + +           V A   G  +G   T L A SEE  ++ G   FP  K+I G+    +P     +        + ++ +VL +    LL    +ILHR     ++  K+   T + ++
Sbjct:   12 QSIFHTIYVHFS-RARLKLYGWRHLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRLNRPYRFTMLLAGILIFVSCMTSAAVPSPGYLFLTHTLIHGIGSTLILCGTSLVTGEYFDKSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQNYGWQVAFQLSGLLFLFVTCLAGIVFISRDMF-----EYHQAISETFEASEENTLDKSLRRDSLLLRNSPKASDGRGWAFCSFEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVSQA----LNDQLVLGARVNLYFGIGESVVFTVGALIGDRIRGYLAFAYFIGASFAALFLLIMQNTYEDINVVY---------VLAGFAGATVGVGNTFLYATSEEIMLVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAVLVSIRVILLGVICYILHRKKKLIIDTEKEHASTNENNL 480          
BLAST of SMED30032324 vs. TrEMBL
Match: A0A4Z2DS50 (Major facilitator superfamily OS=Schistosoma japonicum OX=6182 GN=EWB00_009543 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.403e-22
Identity = 116/492 (23.58%), Postives = 205/492 (41.67%), Query Frame = 1
Query:  199 EKVFNTDVVNWQIRVRLVLFS--------IFACALSIT-VAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMM-LQL---------CLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESF---QKPEITPNKLLGL-TFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYL---LHVSIGFLCILFLV-TVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLL---VFILHRLYTKYLENSKDSKMTIQVSM 1584
            + +F+T  V++  R RL L+         +   AL++  +  K R   +   AL   +N+     ++ W+G  YA +   ++P  T  I      YRF + ++G++  ++C+T++ V  P +  LT + I G GS +      L   + F K +RF V ATA   G   G L    L++ L ++ GW +  QL         CLAG+  I   M      E+   +        +   DKSL  +S      P+ +  +     +F  +  +P VF W   R    ++   ++ L++  +  ++      L  Q        L +  GES+ + +GA  G  I GYL     +   F  +  L+   T++ + +           V A   G  +G   T L A SEE  ++ G   FP  K+I G+    +P     +        + ++ +VL +    LL    +ILHR     ++  K+   T + ++
Sbjct:   12 QSIFHTIYVHFS-RARLKLYGWRHLGWLVLLVAALNVVCIGGKRRAFGIFVAALHKAYNDT-SMTELNWVGDSYAAVGFFLMPFATTAIIRLNRPYRFTMLLAGILIFVSCMTSAAVPSPGYLFLTHTLIHGIGSTLILCGTSLVTGEYFDKSHRFHVLATAFVSGGPYGVLIFGPLYSNLIQNYGWQVAFQLSGLLFLFVTCLAGIVFISRDMF-----EYHQAISETFEASEENTLDKSLRRDSLLLRNSPKASDGRGWAFCSFEHLKNNPQVFVWGFERLLHNLV---IYGLLMNMTAYVSQA----LNDQLVLGARVNLYFGIGESVVFTVGALIGDRIRGYLAFAYFIGASFAALFLLIMQNTYEDINVVY---------VLAGFAGATVGVGNTFLYATSEEIMLVHGSIAFPMTKMIAGIGMLLAPLFSGAIIDGFGYGGFFISMAVLVSIRVILLGVICYILHRKKKLIIDTEKEHASTNENNL 480          
BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000079516.1 (SMESG000079516.1)

HSP 1 Score: 997.268 bits (2577), Expect = 0.000e+0
Identity = 500/501 (99.80%), Postives = 501/501 (100.00%), Query Frame = 1
Query:  118 MQDENEIPXXXXXXXXXXXXFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS 1620
            MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDIL+PQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS
Sbjct:    1 MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIFACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILLPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDSS 501          
BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026518.1 (SMESG000026518.1)

HSP 1 Score: 83.9593 bits (206), Expect = 4.936e-17
Identity = 87/374 (23.26%), Postives = 151/374 (40.37%), Query Frame = 1
Query:  340 HFNNKFPQ---AQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVK---QRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFI-------LGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFL 1422
             FN ++P     ++ WIG  Y+        +   +I Y+   Y     +  L  +I+CIT+S V +P +  LT +   G GS++  +   L V++ F K +R+ + AT L  G ++ S+  + LF++L +   W        G   I   +  + C    +  R     +   S G S  S +                 T+I     P +  W V R   +++  G+   + T S+      +D        +++S +L+ASGE+  YL+GA             L Y+L V+   +C   ++   ++  +  +   + V         G  +G   T L  A EE T +PG   +PF     G     SP L
Sbjct:   44 QFNKEYPNISLTELNWIGDSYSAFGYMSTSLTVTIIIYFNRRYGLFQFLGALWVLISCITSSFVPNPHWLFLTHTLFHGIGSSLIISTAGLVVNEYFDKKHRYHILATTLVSGGSVASILFVMLFSFLIQKYTW-------EGALQILGVLYFIVCGCAAIFFRKNPDLKEVSSTGKSKYSCQ-----------------TYIRWKNIPLLILWFVDRVLTSVVTYGLLLNLTTHSYEKHYSLVD--------SSISTVLFASGEASTYLIGAIIMAITKDLFKERLRYVLLVTSFTMCASLILIYFFEANKTVLRSLLFVA--------GFSLGPSITFLFPAGEELTRLPGHVAYPFSLGGMGFGMMISPLL 377          
BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026692.1 (SMESG000026692.1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.942e-17
Identity = 96/385 (24.94%), Postives = 167/385 (43.38%), Query Frame = 1
Query:  349 NKFPQAQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKS-----LELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGA-------AFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLL 1467
            N    A++ WIG  YA +      + T +I      YR    I   + ++AC+T++ V +  +  LT + + GFGS++  +   L V++ F K +   + AT L  G ++ S+  +  +AYL    GW    + L  ++ +   + +  C     K   K     +GLS KS      +++S Q P +                  +F W + R   +I+  G+  L L D    +   +        +T+L+ +L+A+GE+  YL+G         F +  L Y+L VS   + +     V W+ V      S ++     A ++G C+G   T L  A EE T +PG   +PF     G+    SP L  ++ +I + N + L
Sbjct:   51 NSTSLAELNWIGDSYAALGYISTTLSTALILRSNRRYRLFQFIGAFLILLACLTSAVVPNAHWLFLTHTVLHGFGSSLILSTVGLIVNEYFDKNHPRHILATTLVSGGSVASIVFVQFYAYLIVDYGWRKAFVILGLIYFV---VNLSACFVFARKFSNKSE---EGLSKKSSVNVCRDIKSSQIPLL------------------IF-WTIDRIMTSIVTYGML-LNLADYMRRSESKLT------NSTSLT-VLFAAGEASTYLIGTIITGLTKKFFHNRLKYILLVSTFLMAVSL---VYWEFVSSNKHLSRLL-----AFVSGFCLGPSITFLFPAGEEMTTLPGHMAYPFSLFGMGIGMAVSPILTALIAQIFDYNWFFL 394          
BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026516.1 (SMESG000026516.1)

HSP 1 Score: 77.7962 bits (190), Expect = 5.174e-15
Identity = 85/394 (21.57%), Postives = 165/394 (41.88%), Query Frame = 1
Query:  340 HFNNKFPQ---AQMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLLTFSVLTAANACLLVF 1512
             F+ ++P     ++ WIG  Y+ I      +   +I Y+   Y     +  L  +I+CIT+S V +P +  LT +   G GS++  +   L V++ F K +R+ + AT L  G ++ S+  + LF+ L +H GW    + L  ++ +  ++      ++     +  +G  K L      ++S Q P I                  +F W   R   +++  G+  L LTD       D+         +++  +++ASGE++ YL+G+     I        + ++ ++  ++++   + L+    ++    +     G  +G   T L  A EE T +PG   +P      G     SP L       + + I LL +        C ++F
Sbjct:   45 QFHKEYPNISLTELNWIGDSYSAIGYMSTSLSISIIIYFNRRYGLFQFLGALWVLISCITSSFVPNPHWLFLTHTLFHGIGSSLIISTAGLVVNEYFDKKHRYHILATTLVSGGSVASILFVTLFSKLIEHYGWRKTFIILGIIYFVVCSLAAFFFKKNKDLKEISSKG--KMLCQGQWNIKSIQIPLI------------------IF-WFTDRIMTSVVTYGLL-LNLTDYVYQGHKDL-------MKSSILTIIFASGEAVTYLIGSVITA-ITKDFFKEKLRYILLVTSLSMSICLIILEYTSHIVSIRRLLCFFAGFSLGPSITFLFPAGEEFTRLPGHLAYPVSLGGMGFGMIISPLL-------TAKIIELLDYRWFFFVQGCFILF 401          
BLAST of SMED30032324 vs. Planmine SMEST
Match: SMESG000026519.1 (SMESG000026519.1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.349e-14
Identity = 88/364 (24.18%), Postives = 149/364 (40.93%), Query Frame = 1
Query:  367 QMEWIGRLYAIIVLCMLPIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICGFGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLAKHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSLELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLVLTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAA------------FGYFILGYLLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGTCLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFL 1422
            ++ WIG  YA +      I   +I ++K  Y     +  +  +++C+T+S V +P +  LT +   G GS++  +   L V+D F   + + + AT L  G ++ S+  + LFA L +  GW    + L  L+ I  A   L   +   +V    +        K++        +I    LL L             W + R   +++  G+  L LTD    T  ++        +TTL+  L+ASGE++ YL+GA               Y +LG    +SI   C+          + L+  + ++    +   L G  +G   T L  A EE T +PG   +P      G     SP L
Sbjct:   56 ELNWIGDSYAAVGYMTSGISIAIILFFKRRYGMFQFMGAIWVLVSCVTSSFVPNPHWLFLTHTLFHGIGSSLIISTVGLVVNDHFDSKHPYHILATTLVSGGSVASIIFVTLFASLIQSYGWRYAFIILGCLYFIVNASAALFFRKSNNQVEDTNK--------KTVSCRCEGHIKIIHIPLLCL-------------WFIDRVMTSVVTYGLL-LNLTDYMYRTEKNL------VRSTTLTT-LFASGEAITYLIGATITAITRDFFKDKLRYILLGTSFAMSI---CL----------IALEYFKGIISVCYILCFLAGFTLGPSITFLFPAGEELTRLPGHMAYPISLAGMGFGMLVSPLL 377          
The following BLAST results are available for this feature:
BLAST of SMED30032324 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4V7W33.507e-2324.78Putative monocarboxylate transporter OS=Schistosom... [more]
A0A183A5S63.649e-2326.27MFS domain-containing protein OS=Echinostoma capro... [more]
A0A183REE34.838e-2324.76MFS domain-containing protein OS=Schistosoma rodha... [more]
C1LE481.234e-2223.58Major facilitator superfamily OS=Schistosoma japon... [more]
A0A4Z2DS501.403e-2223.58Major facilitator superfamily OS=Schistosoma japon... [more]
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BLAST of SMED30032324 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032324 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079516.10.000e+099.80SMESG000079516.1[more]
SMESG000026518.14.936e-1723.26SMESG000026518.1[more]
SMESG000026692.19.942e-1724.94SMESG000026692.1[more]
SMESG000026516.15.174e-1521.57SMESG000026516.1[more]
SMESG000026519.18.349e-1424.18SMESG000026519.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032324 ID=SMED30032324|Name=SMED30032324|organism=Schmidtea mediterranea sexual|type=transcript|length=1645bp
GCAGATTGGTTTGCTATGTTCACATTTTGTTTTAATTGTTATTTACCGAA
AGTGTGTTACTCACAATACTTTTGTCAGCCTACACCAAAAACAGAATTTT
AAATCGATAAAATAGAAATGCAAGATGAAAATGAAATTCCGAAACGAAAA
TTGAGTTTGAAGAAATCAAGAAAGAAGTTCAATTCAATACACGAAGAGGA
GAAAGTGTTCAACACGGATGTCGTCAACTGGCAGATCCGCGTCAGATTGG
TCTTGTTCTCAATATTTGCATGTGCGCTATCAATCACGGTCGCAGCTAAG
ATCCGATTTACCCCGGTCATTTCCCATGCACTTTCTGTGCATTTTAACAA
CAAATTCCCTCAAGCTCAAATGGAATGGATTGGGAGGTTATACGCGATTA
TTGTGCTCTGTATGCTGCCAATAGTTACTGGAGTAATAGATTATTATAAA
ATCGGCTATCGGTTTGCACTCTGTATATCGGGACTCATGCACATGATTGC
TTGTATTACAAACTCAACTGTTGTCCATCCATTGTTTTTTGTTTTAACTG
ACTCTTTCATATGCGGCTTTGGATCAGCTATATCATTTGCGGTTTTCCTC
CTAGCTGTCGATGACATATTTCCAAAAGGAAACCGGTTCAGAGTTCCTGC
AACGGCCCTTTGTCAGGGTTCTACAATTGGATCATTATTCATTTTGGACC
TTTTCGCTTATCTTGCGAAACATTTGGGTTGGATGATGTTACAGTTGTGT
TTAGCGGGCCTATTTTCGATCGAAGCCGCCATGTTAATATTAACATGTCC
AGAACACGGGTTGAAAGTTCGTGTGAAACAAAGAGGAGGTAGTAAAGGTC
TAAGTGACAAATCGCTTGAATTGGAAAGTTTCCAAAAACCGGAAATAACT
CCTAATAAATTATTAGGCTTAACATTTATAGAAATGAATTACAGTCCATC
AGTGTTTCCTTGGCTAGTCTACAGATTCTTTATGACGATCATTCATCAAG
GAGTTTTTACTCTCGTTTTAACCGACAGTCACCTCATCACAACGCCAGAC
ATTGACATTCTGATGCCTCAGTATTACGCAACCACTTTGAGCGCCTTGTT
ATATGCTTCAGGCGAATCATTATTTTATCTTCTCGGAGCTGCATTTGGAT
ATTTTATTCTTGGTTACCTTTTGCATGTAAGCATTGGGTTTTTGTGCATC
TTATTTCTTGTAACTGTCACGTGGCAACCGGTGAGACTGCAAATGGCTCA
GTCAATGATGGTTGGAAATGCAGTCTGTGCTTGCTTGACTGGTGTTTGCA
TTGGAGCGTTGGGAACCTGCCTCATCGCTGCTTCTGAGGAGGCAACAATG
ATTCCTGGGAGAAAAATCTTTCCTTTCGTCAAATTAATCGAAGGATTAGC
ATTTACGTTTAGTCCTTTTCTTTTTGACATTTTAAACAAAATCTCTGAAC
AAAACATTTGGTTGTTGACGTTTTCTGTCCTAACAGCAGCAAATGCTTGC
TTATTAGTATTTATTCTGCATAGGTTATACACAAAGTATCTGGAAAACTC
TAAGGACAGTAAGATGACAATACAAGTCTCAATGAGGCCGGTAACACTAT
TATCCAAATTTGATTCTTCATAACTTATAGCTATGTATTTAATCC
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protein sequence of SMED30032324-orf-1

>SMED30032324-orf-1 ID=SMED30032324-orf-1|Name=SMED30032324-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
IKYIAISYEESNLDNSVTGLIETCIVILLSLEFSRYFVYNLCRINTNKQA
FAAVRTENVNNQMFCSEILFKMSKRKGLNVNANPSINLTKGKIFLPGIIV
ASSEAAMRQVPNAPMQTPVKQAQTAFPTIID*
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protein sequence of SMED30032324-orf-2

>SMED30032324-orf-2 ID=SMED30032324-orf-2|Name=SMED30032324-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=502bp
MQDENEIPKRKLSLKKSRKKFNSIHEEEKVFNTDVVNWQIRVRLVLFSIF
ACALSITVAAKIRFTPVISHALSVHFNNKFPQAQMEWIGRLYAIIVLCML
PIVTGVIDYYKIGYRFALCISGLMHMIACITNSTVVHPLFFVLTDSFICG
FGSAISFAVFLLAVDDIFPKGNRFRVPATALCQGSTIGSLFILDLFAYLA
KHLGWMMLQLCLAGLFSIEAAMLILTCPEHGLKVRVKQRGGSKGLSDKSL
ELESFQKPEITPNKLLGLTFIEMNYSPSVFPWLVYRFFMTIIHQGVFTLV
LTDSHLITTPDIDILMPQYYATTLSALLYASGESLFYLLGAAFGYFILGY
LLHVSIGFLCILFLVTVTWQPVRLQMAQSMMVGNAVCACLTGVCIGALGT
CLIAASEEATMIPGRKIFPFVKLIEGLAFTFSPFLFDILNKISEQNIWLL
TFSVLTAANACLLVFILHRLYTKYLENSKDSKMTIQVSMRPVTLLSKFDS
S*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 49..407
e-value: 1.2E-8
score: 34.4
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 35..236
e-value: 4.5E-7
score: 31.4
NoneNo IPR availableCDDcd17352MFS_MCT_SLC16coord: 44..216
e-value: 1.61621E-4
score: 41.7693
NoneNo IPR availableTMHMMTMhelixcoord: 45..67
NoneNo IPR availableTMHMMTMhelixcoord: 114..136
NoneNo IPR availableTMHMMTMhelixcoord: 279..301
NoneNo IPR availableTMHMMTMhelixcoord: 203..225
NoneNo IPR availableTMHMMTMhelixcoord: 382..404
NoneNo IPR availableTMHMMTMhelixcoord: 449..471
NoneNo IPR availableTMHMMTMhelixcoord: 417..439
NoneNo IPR availableTMHMMTMhelixcoord: 345..367
NoneNo IPR availableTMHMMTMhelixcoord: 176..198
NoneNo IPR availableTMHMMTMhelixcoord: 87..107
NoneNo IPR availableTMHMMTMhelixcoord: 141..163
NoneNo IPR availableTMHMMTMhelixcoord: 316..338
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 36..469