SMED30032317

Overview
NameSMED30032317
Smed IDSMED30032317
Length (bp)1352
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032317 (SMED30032317) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032317 (SMED30032317) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032317

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30032317h1SMcG0001681 dd_Smed_v4_10106_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30032317 vs. Ensembl Human
Match: NOTCH3 (notch receptor 3 [Source:HGNC Symbol;Acc:HGNC:7883])

HSP 1 Score: 52.373 bits (124), Expect = 1.629e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +    C CG  Y+G  C+  +N C    C N+  C  +IG+ TCI +
Sbjct:  366 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICM 458
[Close]
BLAST of SMED30032317 vs. Ensembl Human
Match: NOTCH1 (notch receptor 1 [Source:HGNC Symbol;Acc:HGNC:7881])

HSP 1 Score: 52.373 bits (124), Expect = 1.869e-6
Identity = 33/104 (31.73%), Postives = 46/104 (44.23%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLDQKELVSNQGN 875
            CD+N   G  IC     +     + D D C+     C + G C+N     EC C   Y+G  C+I +N C  + C N   C  QIG+  CI +   E V  + N
Sbjct:  387 CDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVN 490
[Close]
BLAST of SMED30032317 vs. Ensembl Xenopus
Match: NOTCH3 (notch 3 [Source:NCBI gene;Acc:100494244])

HSP 1 Score: 52.7582 bits (125), Expect = 1.375e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +   +C CG  Y+G  C+  +N C    C N   C  +IG+ TCI +
Sbjct:  305 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHFGRCVNTQGSFQCQCGRGYTGPRCETDVNECLSTPCQNDATCLDRIGEFTCICM 397
[Close]
BLAST of SMED30032317 vs. Ensembl Xenopus
Match: NOTCH3 (notch 3 [Source:NCBI gene;Acc:100494244])

HSP 1 Score: 52.373 bits (124), Expect = 1.528e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +   +C CG  Y+G  C+  +N C    C N   C  +IG+ TCI +
Sbjct:  238 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHFGRCVNTQGSFQCQCGRGYTGPRCETDVNECLSTPCQNDATCLDRIGEFTCICM 330
[Close]
BLAST of SMED30032317 vs. Ensembl Xenopus
Match: NOTCH3 (notch 3 [Source:NCBI gene;Acc:100494244])

HSP 1 Score: 52.373 bits (124), Expect = 1.605e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +   +C CG  Y+G  C+  +N C    C N   C  +IG+ TCI +
Sbjct:  318 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHFGRCVNTQGSFQCQCGRGYTGPRCETDVNECLSTPCQNDATCLDRIGEFTCICM 410
[Close]
BLAST of SMED30032317 vs. Ensembl Xenopus
Match: NOTCH3 (notch 3 [Source:NCBI gene;Acc:100494244])

HSP 1 Score: 52.373 bits (124), Expect = 1.838e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +   +C CG  Y+G  C+  +N C    C N   C  +IG+ TCI +
Sbjct:  350 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHFGRCVNTQGSFQCQCGRGYTGPRCETDVNECLSTPCQNDATCLDRIGEFTCICM 442
[Close]
BLAST of SMED30032317 vs. Ensembl Mouse
Match: Notch3 (notch 3 [Source:MGI Symbol;Acc:MGI:99460])

HSP 1 Score: 52.373 bits (124), Expect = 1.081e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +    C CG  Y+G  C+  +N C    C N+  C  +IG+ TCI +
Sbjct:  367 CDTNPVSGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICM 459
[Close]
BLAST of SMED30032317 vs. UniProt/SwissProt
Match: sp|Q9R172|NOTC3_RAT (Neurogenic locus notch homolog protein 3 OS=Rattus norvegicus OX=10116 GN=Notch3 PE=2 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 7.366e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +    C CG  Y+G  C+  +N C    C N+  C  +IG+ TCI +
Sbjct:  368 CDTNPVSGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICM 460
[Close]
BLAST of SMED30032317 vs. UniProt/SwissProt
Match: sp|Q61982|NOTC3_MOUSE (Neurogenic locus notch homolog protein 3 OS=Mus musculus OX=10090 GN=Notch3 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.559e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +    C CG  Y+G  C+  +N C    C N+  C  +IG+ TCI +
Sbjct:  367 CDTNPVSGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICM 459
[Close]
BLAST of SMED30032317 vs. UniProt/SwissProt
Match: sp|Q9UM47|NOTC3_HUMAN (Neurogenic locus notch homolog protein 3 OS=Homo sapiens OX=9606 GN=NOTCH3 PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 7.825e-6
Identity = 31/93 (33.33%), Postives = 42/93 (45.16%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISL 842
            CD+N   G  IC     F       D D C+     C + G CVN +    C CG  Y+G  C+  +N C    C N+  C  +IG+ TCI +
Sbjct:  366 CDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICM 458
[Close]
BLAST of SMED30032317 vs. UniProt/SwissProt
Match: sp|P46531|NOTC1_HUMAN (Neurogenic locus notch homolog protein 1 OS=Homo sapiens OX=9606 GN=NOTCH1 PE=1 SV=4)

HSP 1 Score: 52.373 bits (124), Expect = 8.977e-6
Identity = 33/104 (31.73%), Postives = 46/104 (44.23%), Query Frame = 3 
Query:  573 CDSN---GNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLDQKELVSNQGN 875
            CD+N   G  IC     +     + D D C+     C + G C+N     EC C   Y+G  C+I +N C  + C N   C  QIG+  CI +   E V  + N
Sbjct:  387 CDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVN 490
[Close]
BLAST of SMED30032317 vs. TrEMBL
Match: A0A1Z1R174 (Jagged-like (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.168e-15
Identity = 70/258 (27.13%), Postives = 117/258 (45.35%), Query Frame = 3 
Query:   69 FVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNV------TLDSTVNTTKMRRE------LYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKFSTKKSFLLNNVRYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYC 806
            F+ +++   +   A  +APGT+T+ I+       Y +D V       L S V + +   E      L   ++    +     E  I F+V N+ +   +N  +   QN    +T  F          K+ ++ N+ Y  +  +S+++  L V   +KCK  F+P+  C V C +  ++ KCD NG KIC  G+ F+  T+ C+ D C     IC N+G CV+     +C C   +SG  C+I  + C +  C N   C
Sbjct:    5 FILLSINFLNNISAIKRAPGTLTLKIQTP----AYVEDCVEKYCKYVLKSCVGSGRSENERCLWSPLTVNSNYFHKMPKYLAE--ISFEVDNVKDPFYFNANLSYFQNEILVKTENF----------KNIIIQNL-YGASQIHSQDFATLSVDIVIKCKPGFFPKNRCLVSCFEEASVYKCDENGRKICLSGYVFDPLTNKCNLDNCNSENIICFNNGTCVSGAHNPKCFCVDGFSGPSCEI-WDPCTKPRCKNGGKC 244
[Close]
BLAST of SMED30032317 vs. Ensembl Cavefish
Match: crb1 (crumbs cell polarity complex component 1 [Source:NCBI gene;Acc:103040357])

HSP 1 Score: 50.447 bits (119), Expect = 3.916e-6
Identity = 26/85 (30.59%), Postives = 41/85 (48.24%), Query Frame = 3 
Query:  615 FNDYTHTCDTDW----CTQSKPICVND----GHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLD 845
            F+ +  TC + W    C  S  IC +     G+C     + ECVC   Y+G  C++ +++C QH C N   C   +G+  C+  D
Sbjct: 1163 FDLFNCTCPSGWVGQRCEISTDICASSPCHHGNCTAHGLQYECVCELGYTGVNCELVVDVCAQHQCANGGTCLHGVGQYACLCAD 1247
[Close]
BLAST of SMED30032317 vs. Planmine SMEST
Match: SMESG000059836.1 (SMESG000059836.1)

HSP 1 Score: 798.119 bits (2060), Expect = 0.000e+0
Identity = 410/412 (99.51%), Postives = 411/412 (99.76%), Query Frame = 3 
Query:   54 MLKIYFVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDSTVNTTKMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKFSTKKSFLLNNVRYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLDQKELVSNQGNNYFKVMSAYTILSMFGXXXXXXXXXXXXXWACKRQRFERYRSMDASIHSSHTYYPNNSLRKASKCCLHCDHENNPALNMYNSIYGNRAFYSLRSPNRIHNSLNRAFSDILIPYDDKRLSMKSVXXXXXXXXXXXXIED 1289
            MLKIYFVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDSTVNTTKMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKFSTKKSFLLNN+RYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYT TCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLDQKELVSNQGNNYFKVMSAYTILSMFGFVIVFIITILLTIWACKRQRFERYRSMDASIHSSHTYYPNNSLRKASKCCLHCDHENNPALNMYNSIYGNRAFYSLRSPNRIHNSLNRAFSDILIPYDDKRLSMKSVSMPEESLPLPPPIED
Sbjct:    1 MLKIYFVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDSTVNTTKMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKFSTKKSFLLNNMRYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTQTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQIGKVTCISLDQKELVSNQGNNYFKVMSAYTILSMFGFVIVFIITILLTIWACKRQRFERYRSMDASIHSSHTYYPNNSLRKASKCCLHCDHENNPALNMYNSIYGNRAFYSLRSPNRIHNSLNRAFSDILIPYDDKRLSMKSVSMPEESLPLPPPIED 412
[Close]
BLAST of SMED30032317 vs. Planmine SMEST
Match: SMESG000005484.1 (SMESG000005484.1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.102e-19
Identity = 70/258 (27.13%), Postives = 116/258 (44.96%), Query Frame = 3 
Query:   69 FVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNV------TLDSTVNTTKMRRE------LYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKFSTKKSFLLNNVRYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYC 806
            F+ +++   +   A  +APGT+T+ I+       Y +D V       L S V + +   E      L   ++    +     E  I F+V N+ +   +N  +   QN    +T  F          K+ ++ N+ Y     +S+++  L V   +KCK  F+P+  C V C +  ++ KCD NG KIC  G+ F+  T+ C+ D C     IC N+G CV+     +C C   +SG  C+I  + C +  C N   C
Sbjct:   11 FILLSINFLNNISAIKRAPGTLTLKIQTP----AYVEDCVEKYCKYVLKSCVGSGRSENERCLWSPLTVNSNYFHKMPKYLAE--ISFEVDNVKDPFYFNANLSYFQNEILVKTENF----------KNIIIQNL-YGATQIHSQDFATLSVDIVIKCKPGFFPKNRCLVSCFEEASVYKCDENGRKICLSGYVFDPLTNKCNLDNCNSENIICFNNGTCVSGAHNPKCFCVDGFSGPSCEI-WDPCAKPRCKNGGKC 250
[Close]
BLAST of SMED30032317 vs. Planmine SMEST
Match: SMESG000025147.1 (SMESG000025147.1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.792e-15
Identity = 63/254 (24.80%), Postives = 113/254 (44.49%), Query Frame = 3 
Query:   63 IYFVFV-ALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDSTVNTTKMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKI-MQNGRKTETYEFRLISFK-FSTKKSFLLNNVRYVTATAYSKEYKELEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCSVQ 815
            ++F F  +L I+   F   Q+P  + +         L   + V     ++     +  +     ++L  +   E+  +F + N+    D N  + I ++NG  T  +  + I+ K  S+K +F L+++        + E++         C+  +YP   C+  C  ++    CD  GNKIC++G YF+   + C  + C  S   C N+G CVN+    EC C   YSGK+C+   N C  + C N   C ++
Sbjct:   16 LFFGFSYSLHITQANFNSQQSPAKVNLRFLISKFSQLLTSEPVKFSLCIDKNH-EKCFWTTNKNVELHEELFQEVQFEFYINNM----DTNLSISIKLENGGSTINFSHKTINLKKISSKDTFELHSITLENFVKVTVEHE---------CENGYYPAKQCNKFCFPSENEYTCDGFGNKICKEGLYFDADENKCLPNNCIASSIQCYNNGFCVNKGSTFECKCMESYSGKFCEFA-NPCFPNPCQNMGKCFIK 254
[Close]
BLAST of SMED30032317 vs. Planmine SMEST
Match: SMESG000059880.1 (SMESG000059880.1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.691e-15
Identity = 68/251 (27.09%), Postives = 116/251 (46.22%), Query Frame = 3 
Query:   72 VFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDSTVNTT--KMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQNGRKTETYEFRLISFKF--STKKSFLLNNVRYVTATAYSKEYKE-LEVMTSVKCKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWCTQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTYCS 809
            +F  + +  +        GTI M   +  D   +G + + L S + +      + LY + ++M + +  A + TIKFD+ +IS    + G + I +N      Y+F   SF++  S K +   N+V YV+ + YS+  K+ L+V+ + KCK N++P+F C+  C K  +   CD NG  IC  G+Y +  +  C+     QS  +       V  R+           G  C +   +C+   C N T CS
Sbjct:    7 IFFVVELLCILLPENDTLGTIEMEFSFQTD-ESFGIE-IFLSSFIFSLVHNEHKTLYNEINKM-IFNGEASKRTIKFDIDDISTIFYHEGTLIISKNTENKSLYQFNTQSFQYVLSNKLNVKKNSVIYVSKSIYSRNLKKNLDVLLTGKCKPNYFPKFICTQFC-KRNSHSVCDINGKIICHSGYYLSKNSQKCEKIVNPQSLTL-------VKIRRMKNQKSHEKNKGIKCNVEKFICELDICHNVTRCS 246
The following BLAST results are available for this feature:
BLAST of SMED30032317 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
NOTCH31.629e-633.33notch receptor 3 [Source:HGNC Symbol;Acc:HGNC:7883... [more]
NOTCH11.869e-631.73notch receptor 1 [Source:HGNC Symbol;Acc:HGNC:7881... [more]
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BLAST of SMED30032317 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
NOTCH31.375e-633.33notch 3 [Source:NCBI gene;Acc:100494244][more]
NOTCH31.528e-633.33notch 3 [Source:NCBI gene;Acc:100494244][more]
NOTCH31.605e-633.33notch 3 [Source:NCBI gene;Acc:100494244][more]
NOTCH31.838e-633.33notch 3 [Source:NCBI gene;Acc:100494244][more]
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BLAST of SMED30032317 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Notch31.081e-633.33notch 3 [Source:MGI Symbol;Acc:MGI:99460][more]
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BLAST of SMED30032317 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9R172|NOTC3_RAT7.366e-633.33Neurogenic locus notch homolog protein 3 OS=Rattus... [more]
sp|Q61982|NOTC3_MOUSE7.559e-633.33Neurogenic locus notch homolog protein 3 OS=Mus mu... [more]
sp|Q9UM47|NOTC3_HUMAN7.825e-633.33Neurogenic locus notch homolog protein 3 OS=Homo s... [more]
sp|P46531|NOTC1_HUMAN8.977e-631.73Neurogenic locus notch homolog protein 1 OS=Homo s... [more]
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BLAST of SMED30032317 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A1Z1R1741.168e-1527.13Jagged-like (Fragment) OS=Schmidtea mediterranea O... [more]
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BLAST of SMED30032317 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
crb13.916e-630.59crumbs cell polarity complex component 1 [Source:N... [more]
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BLAST of SMED30032317 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032317 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000059836.10.000e+099.51SMESG000059836.1[more]
SMESG000005484.13.102e-1927.13SMESG000005484.1[more]
SMESG000025147.12.792e-1524.80SMESG000025147.1[more]
SMESG000059880.17.691e-1527.09SMESG000059880.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032317 ID=SMED30032317|Name=SMED30032317|organism=Schmidtea mediterranea sexual|type=transcript|length=1352bp
ATTCAACATTATATTCCATTTGTTTATAAATTTTTGTCGTTGATCAATAG
CAAATGTTGAAAATTTATTTCGTGTTTGTAGCTTTAGGAATTTCATCTGT
TGGGTTCGCAAGATTTCAGGCTCCCGGAACTATCACAATGAACATAAAAT
ACCACCCGGATATCTATTTGTATGGCGATGACAATGTGACTCTGGATTCG
ACTGTAAATACAACTAAAATGAGAAGAGAATTATACAAAAAAGCTGACCG
AATGAAGTTATTAAGTGATGGTGCTCTGGAACTCACAATTAAATTTGATG
TATATAACATATCAGAACATATTGACTATAATGGACTTATGAAAATAATG
CAAAATGGCAGAAAGACCGAAACGTATGAATTCCGATTGATCAGTTTTAA
ATTCTCCACAAAGAAATCATTTTTATTAAATAATGTGCGTTACGTAACCG
CAACTGCGTACAGTAAGGAATACAAGGAGCTCGAAGTAATGACATCTGTC
AAATGTAAGCAAAACTTTTATCCCGAATTTAATTGCTCTGTATTATGCGT
CAAAACACAAACCATTGAGAAATGTGACTCAAATGGCAATAAGATTTGCC
GCAAAGGCTTTTATTTCAATGATTATACTCATACGTGTGATACCGATTGG
TGTACACAATCAAAACCCATTTGCGTCAATGATGGTCATTGCGTCAACCG
TAGAAAGGAATCTGAGTGTGTTTGTGGCCCATTGTACTCCGGTAAATATT
GTCAGATCCCCCTCAATTTATGCCAGCAACATAATTGTCCCAATAAAACA
TATTGTTCTGTACAAATTGGCAAAGTGACTTGCATAAGTTTGGATCAAAA
GGAATTAGTTTCAAATCAAGGAAATAATTATTTTAAGGTAATGTCTGCTT
ACACAATACTCTCAATGTTTGGATTTGTGATCGTGTTCATCATAACAATT
TTGTTAACCATATGGGCTTGCAAAAGACAGAGATTCGAAAGATACAGGTC
AATGGACGCCAGTATTCATTCGAGTCACACTTACTATCCTAATAATTCTT
TGAGAAAAGCATCGAAATGTTGTCTTCATTGCGACCATGAAAATAACCCA
GCGTTGAACATGTATAATTCTATCTACGGAAACAGAGCCTTTTATTCATT
AAGAAGTCCAAATCGTATACACAATTCATTAAACCGAGCTTTTAGTGATA
TATTGATACCATATGATGACAAGCGTTTGAGTATGAAGTCAGTTTCCATG
CCCGAGGAATCCCTACCTTTACCCCCTCCAATAGAGGACTAACGGATATT
AATCAAGATTGGATAGTGTTGGAAAATTCTCTATAAAAATGTTGTGGACA
GA
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protein sequence of SMED30032317-orf-1

>SMED30032317-orf-1 ID=SMED30032317-orf-1|Name=SMED30032317-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=413bp
MLKIYFVFVALGISSVGFARFQAPGTITMNIKYHPDIYLYGDDNVTLDST
VNTTKMRRELYKKADRMKLLSDGALELTIKFDVYNISEHIDYNGLMKIMQ
NGRKTETYEFRLISFKFSTKKSFLLNNVRYVTATAYSKEYKELEVMTSVK
CKQNFYPEFNCSVLCVKTQTIEKCDSNGNKICRKGFYFNDYTHTCDTDWC
TQSKPICVNDGHCVNRRKESECVCGPLYSGKYCQIPLNLCQQHNCPNKTY
CSVQIGKVTCISLDQKELVSNQGNNYFKVMSAYTILSMFGFVIVFIITIL
LTIWACKRQRFERYRSMDASIHSSHTYYPNNSLRKASKCCLHCDHENNPA
LNMYNSIYGNRAFYSLRSPNRIHNSLNRAFSDILIPYDDKRLSMKSVSMP
EESLPLPPPIED*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013032EGF-like_CS
IPR000742EGF-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0004872receptor activity
GO:0005509calcium ion binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0000122negative regulation of transcription from RNA polymerase II promoter
GO:0001525angiogenesis
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO:0007219Notch signaling pathway
GO:0007275multicellular organism development
GO:0030154cell differentiation
GO:0050793regulation of developmental process
GO:0060271cilium morphogenesis
GO:0061314Notch signaling involved in heart development
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 198..267
e-value: 8.1E-8
score: 34.7
NoneNo IPR availableCDDcd00054EGF_CAcoord: 196..234
e-value: 0.0020427
score: 33.7642
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.626
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 196..235
NoneNo IPR availableTMHMMTMhelixcoord: 283..305
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 222..233
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 196..234
score: 14.768