FMN_red domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032272 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of FMN_red domain-containing protein vs. TrEMBL
Match: A0A5J4N8B1 (FMN_red domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0011101 PE=4 SV=1) HSP 1 Score: 141.354 bits (355), Expect = 9.003e-38 Identity = 86/198 (43.43%), Postives = 126/198 (63.64%), Query Frame = 1 Query: 43 KLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQS-KAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHT-EDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNN 630 + L+F SSVR R +RV K+A R KG SV V+DPL LPL+ NP HFY + + +AP+QL L + V AD + T EYN +PPAL NLIDH P ++AFKP GI++YS G GG+ S++ L L+ +GCLV P V IS V T++A+G+ G T + +++DQ+E++G A+K + S + P++++ Sbjct: 9 RTLLFASSVRPGRMNDRVLKLAERRLELKGHSVTVVDPLHWTLPLIVNPWHFYANPAEQAPKQLKDLFELVSSADRLIFTTAEYNRSIPPALSNLIDHLPHSAYAFKPTGIIAYSVGSGGQLSAAQLRLMLSDLGCLVVPHHVVISNVDRTISADGEPSGRKTIIGEVDLMIDQVEYIGNAIKCNRTSTCAEPPKVHS 206
BLAST of FMN_red domain-containing protein vs. TrEMBL
Match: A0A1S3KEB9 (uncharacterized protein LOC106181199 OS=Lingula unguis OX=7574 GN=LOC106181199 PE=4 SV=1) HSP 1 Score: 128.642 bits (322), Expect = 6.570e-33 Identity = 74/187 (39.57%), Postives = 111/187 (59.36%), Query Frame = 1 Query: 40 LKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQ-GGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQ 597 LK+++FL SVRD+R ERV + E V++MDP+K P+LK PLH Y D+SKAPQ L N K+ AD ++++T EYN+ +PPAL N++DHF +++KP+GI+ YS GQ GG ++ L L +GCL I I V + ++ EG+ H LL Q+++ A+K ++ Sbjct: 6 LKVVLFLGSVRDNRLGERVATFMKNILVETNHEVEIMDPMKTEFPMLKKPLHHYQDKSKAPQFLQDCNAKIQAADAFVVVTAEYNHSIPPALSNMMDHFGSSGYSYKPSGIVCYSPGQYGGMRAAMQLRSFLGELGCLSVSNIFGIPQVMEAISPEGEPQNEHMRKGALRLLAQLDWHAQAMKNHRE 192
BLAST of FMN_red domain-containing protein vs. TrEMBL
Match: K1PHZ2 (Uncharacterized protein yieF OS=Crassostrea gigas OX=29159 GN=CGI_10012025 PE=4 SV=1) HSP 1 Score: 126.331 bits (316), Expect = 4.796e-32 Identity = 72/188 (38.30%), Postives = 109/188 (57.98%), Query Frame = 1 Query: 40 LKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQK-VMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTG-IGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQ 597 LK+L+ L S+RD R RV K R E+ F V DP++ PL+ P+HFY + K + V N+K V AD Y++++ EYN+ VPPAL NL+D FP ++++KP+G++ YS GQ G ++ +TG +GCL I I VQ+ EG+ H E K +LDQ+E++ A+K ++ Sbjct: 7 LKVLLILGSIRDGRQGLRVAKFMRRKLEERNFDVKFFDPVEMKFPLITQPVHFYGPERKGAPAILVENEKFVKDADAYVIVSAEYNHNVPPALTNLLDAFPCSAYSYKPSGLVCYSMGQFGGMRAAMALRAITGELGCLSVSNIFGIPTVQNAFDEEGNPKNDHMESGAKKMLDQLEWMATAMKNHRE 194
BLAST of FMN_red domain-containing protein vs. TrEMBL
Match: H9LIY4 (NAD(P)H:quinone oxidoreductase-like protein OS=Crassostrea ariakensis OX=94323 PE=2 SV=1) HSP 1 Score: 123.25 bits (308), Expect = 7.248e-31 Identity = 71/188 (37.77%), Postives = 108/188 (57.45%), Query Frame = 1 Query: 40 LKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFY-PDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTG-IGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQ 597 LK+L+ L S+RD R RV K R E+ F V DP++ PL+ P+HFY P++ AP L + V AD Y++++ EYN+ VPPAL NL+D FP ++++KP+G++ YS G G ++ +TG +GCL I I VQ+ EG+ H E K +LDQ+E++ A+K ++ Sbjct: 7 LKVLLVLGSIRDGRQGLRVAKFMKRKLEERNFDVKFFDPVEMKFPLITQPVHFYGPERKGAPAILAENEKFVKEADAYVIVSAEYNHNVPPALTNLLDAFPCSAYSYKPSGLVCYSMGPFGGMRAAMALRAITGELGCLSVSNIFGIPTVQNAFDEEGNPQNDHMESGAKKMLDQLEWMATAMKNHRE 194
BLAST of FMN_red domain-containing protein vs. TrEMBL
Match: A0A1D1UJ34 (FMN_red domain-containing protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_00441-1 PE=4 SV=1) HSP 1 Score: 122.094 bits (305), Expect = 2.449e-30 Identity = 69/197 (35.03%), Postives = 120/197 (60.91%), Query Frame = 1 Query: 34 SGLKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQ-GGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGD--AHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQ 615 SGL +++FL SVR++R A RV K +L ++ +V+++DP +L + PLHFY D ++AP LH LN K+ AD ++++ EYN + PAL +++D+FPP S+A++P+ I++YS GQ GG ++ L L+ + + P + I V + +T +G+ A G T+ + + + Q E+ ALK ++ S + + Sbjct: 5 SGLNIMVFLGSVRENRMATRVGKFIQKLLEKRSHTVEIVDPKALDLGEVIQPLHFYKDPAEAPAILHELNDKIKKADGFVVVAAEYNSAISPALCSIMDNFPPLSYAYRPSAIVTYSMGQFGGIRAAMQLRQFLSELTTVHMPSMTVIPKVHEAITEDGEVTAEGPLTKAS-EAMCKQFEWYARALKNERNSSGIPK 200
BLAST of FMN_red domain-containing protein vs. Planmine SMEST
Match: SMESG000023871.1 (SMESG000023871.1) HSP 1 Score: 385.956 bits (990), Expect = 1.399e-137 Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 1 Query: 31 MSGLKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF 636 MSGLKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDPLKENLPLLKNPLHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF Sbjct: 1 MSGLKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDPLKENLPLLKNPLHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF 202
BLAST of FMN_red domain-containing protein vs. Planmine SMEST
Match: SMESG000023871.1 (SMESG000023871.1) HSP 1 Score: 336.65 bits (862), Expect = 1.603e-118 Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 1 Query: 103 MALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF 636 MALRLATEKGFSVDVMDPLKENLPLLKNPLHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF Sbjct: 1 MALRLATEKGFSVDVMDPLKENLPLLKNPLHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILLDQIEFVGLALKQQKQSGKVKQPRLNNVF 178
BLAST of FMN_red domain-containing protein vs. Planmine SMEST
Match: SMESG000060640.1 (SMESG000060640.1) HSP 1 Score: 120.168 bits (300), Expect = 3.991e-33 Identity = 66/190 (34.74%), Postives = 109/190 (57.37%), Query Frame = 1 Query: 40 LKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDXXXXXXXXXXXXXHFYPDQSKAPQQLHVLNQKVMFADCYLMITGEYNYGVPPALLNLIDHFPPPSFAFKPAGILSYSAGQGGRHSSSHLEHTLTGIGCLVQPFIVNISVVQDTVTAEGDAHGGHTEDNIKILL-DQIEFVGLALKQQKQSGK 606 + + + LSSVR RNA+R+ M L V+V+DPL +PLL P H+YPD+ P+ L+ LN+ + +D + +T EYN VPP L+NLID P S+ +KPA I++YS G GG + L L+ +GC++ P +I+ + + +T+ G+ E IL+ Q++++ A++ ++ K Sbjct: 13 ISITLILSSVRPHRNADRICAMMKSLLQSFNCRVEVIDPLIIGIPLLDVPYHYYPDKKDIPEWLNNLNKSIQESDALIFLTTEYNRAVPPPLINLIDFIPFSSYEYKPASIITYSVGAGGGSALDQLRSILSYLGCIITPLCPSITRMDEKITSNGEIDPKCIEKRSLILMCQQLQYITHAIRSWRRKNK 202 The following BLAST results are available for this feature:
BLAST of FMN_red domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of FMN_red domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of FMN_red domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of FMN_red domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032272 ID=SMED30032272|Name=FMN_red domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=788bpback to top protein sequence of SMED30032272-orf-1 >SMED30032272-orf-1 ID=SMED30032272-orf-1|Name=SMED30032272-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=203bp MSGLKLLIFLSSVRDSRNAERVYKMALRLATEKGFSVDVMDPLKENLPLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|